WO2019098811A2 - Chp(시클로-히스프로)를 포함하는 골 손실 질환의 예방, 개선 또는 치료용 조성물 - Google Patents

Chp(시클로-히스프로)를 포함하는 골 손실 질환의 예방, 개선 또는 치료용 조성물 Download PDF

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WO2019098811A2
WO2019098811A2 PCT/KR2018/014275 KR2018014275W WO2019098811A2 WO 2019098811 A2 WO2019098811 A2 WO 2019098811A2 KR 2018014275 W KR2018014275 W KR 2018014275W WO 2019098811 A2 WO2019098811 A2 WO 2019098811A2
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Prior art keywords
chp
bone loss
bone
composition
present
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Ceased
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PCT/KR2018/014275
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English (en)
French (fr)
Korean (ko)
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WO2019098811A3 (ko
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정회윤
이헌종
이도현
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Novmetapharma Co Ltd
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Novmetapharma Co Ltd
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Priority to JP2020527807A priority Critical patent/JP6944221B2/ja
Priority to EP18879826.8A priority patent/EP3714885B1/en
Priority to CN201880074979.3A priority patent/CN111372586B/zh
Priority to US16/764,913 priority patent/US11975041B2/en
Publication of WO2019098811A2 publication Critical patent/WO2019098811A2/ko
Publication of WO2019098811A3 publication Critical patent/WO2019098811A3/ko
Anticipated expiration legal-status Critical
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4985—Pyrazines or piperazines ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/30—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/12—Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/306—Foods, ingredients or supplements having a functional effect on health having an effect on bone mass, e.g. osteoporosis prevention

Definitions

  • the present invention relates to a composition for preventing, ameliorating or treating bone loss diseases including CHP (cyclohexane), and more particularly to a composition for preventing, improving or treating a bone loss disease including CHP or a prostate extract containing the same, ≪ / RTI >
  • Bone modeling and remodeling plays an important role in bone development, growth, and metabolism.
  • the bone formation starts from the birth and continues until the young adult years, when the skeleton matures and the growth ends, forming the maximum bone mass from the 20s to the early 30s. After about 30 years, the bone is removed and the bone is replenished again.
  • the bone formation and the bone absorption are balanced with each other. After this period, bone loss due to bone resorption can not be fully followed by bone formation, resulting in bone loss of about 0.3 ⁇ 0.5% per year. Especially in women, Loss.
  • Bone tissue constitutes the cartilage and skeletal system. It plays a role of support and muscle attachment by mechanical function, protects organism and bone marrow, and preserve calcium and phosphorus to maintain homeostasis. Bone tissue consists of several types of cells, such as collagen, glycoprotein, and osteoblast, osteoclast, and osteocyte.
  • bone tissue is a dynamic tissue formed by osteoblasts, which is continuously repeatedly absorbed and destroyed by osteoclasts.
  • Osteoporosis is a disease caused by the collapse of the balance between osteoblasts and osteoclasts, The lime of the bone is thinned and the density of the bone is thinned, thereby expanding the bone marrow cavity. As the condition progresses, the bones become weak, so that even a small impact is liable to fracture.
  • Osteoporosis is a cause of 15% of the deaths of the elderly as a result of various fractures, especially femoral fractures or vertebral fractures, which are easily caused by the weakening of the bone rather than the symptom itself, .
  • the bone mass is affected by various factors such as genetic factors, nutritional intake, hormonal changes, exercise and lifestyle differences, and the causes of osteoporosis include age, lack of exercise, low birth weight, smoking, low calcium diet, Ovariectomy and the like are known.
  • age lack of exercise
  • low birth weight low birth weight
  • smoking, low calcium diet, Ovariectomy and the like are known.
  • bone reduction continues after 30 years of age, and bone turnover is rapidly progressed by hormonal changes in menopause.
  • osteoporosis is an unavoidable symptom for elderly people, especially postmenopausal women.
  • interest in osteoporosis and its therapeutic agents is gradually increasing.
  • osteoporosis is a disease that can not be treated only by short-term administration of a drug, and long-term administration of a drug is essential. Therefore, there is a desperate need to develop new materials effective for prevention and treatment of osteoporosis with new action and skeleton structure and less toxicity and side effects.
  • Another object of the present invention is to provide a pharmaceutical composition for preventing or treating bone loss diseases including prostate extract.
  • the present invention provides a pharmaceutical composition for preventing or treating a bone loss disease comprising a cyclo-Hispro or a pharmaceutically acceptable salt thereof.
  • the present invention also provides a health functional food composition for prevention or amelioration of bone loss diseases including cyclo-hydroprol.
  • the present invention also provides a pharmaceutical composition for preventing or treating bone loss diseases comprising prostate extract.
  • the present invention also provides a health functional food composition for prevention or improvement of bone loss diseases comprising prostate extract.
  • the bone loss disease may be any one selected from the group consisting of osteoporosis, Paget's disease, alveolar bone loss, osteomalacia, and renal osteodystrophy.
  • the osteoporosis may be caused by female hormone reduction.
  • the composition for preventing, ameliorating or treating bone loss diseases including the cyclo-hepto or prostate extract of the present invention, is excellent in the promotion of osteoblast differentiation and bone mineral density enhancement, and thus is effective for the treatment of bone loss diseases.
  • FIG. 1 is a graph showing changes in bone density of the femur by administration of CHP (cyclosporine).
  • Fig. 2 is a graph showing changes in bone volume ratio due to the administration of CHP (cyclosporine).
  • FIG. 3 shows the effect of promoting the differentiation of osteoblasts by CHP (cyclo-hepro) treatment.
  • cyclo-heaps are effective in preventing, ameliorating or treating bone loss diseases through osteoporosis improvement effect and osteoblast differentiation promoting effect in an animal model of ovariectomy, and completed the present invention .
  • the present invention provides a pharmaceutical composition for the prevention or treatment of bone loss diseases, including cyclo-heath or prostate extract.
  • the present invention also provides a health functional food composition for preventing or ameliorating a bone loss disease including a cyclo-heath or prostate extract.
  • Cyclo-HisPro is a naturally occurring circular dipeptide consisting of Histidine-Proline, a metabolite of thyrotropin-releasing hormone (TRH) It is a physiologically active dipeptide that is synthesized in the body by TRN metabolism and de novo. It is widely distributed in the brain, spinal cord and gastrointestinal tract.
  • CHP may be synthesized or commercially available. Further, it can be used after purification from a substance containing CHP, for example, a prostate extract.
  • &quot purified " it is meant that CHP is in a concentrated form compared to the form obtainable from natural sources such as prostate extract.
  • the purified components can be obtained from their natural source or obtained by chemical synthesis methods.
  • the main constituents of the above-mentioned “prostate extract” are zinc, CHP, prostaglandin precursor and arachidonic acid. Since the CHP is contained at a high concentration, the effect of increasing CHP-induced bone mineral density and bone volume ratio, The effect can be reasonably expected.
  • the " prostate extract” may be a bovine or porcine prostate powder and the like, preferably in the form of removing fat to increase the content of CHP, but is not limited thereto.
  • the term " bone loss " means a symptom of loss of bone caused by imbalance of osteoclast and osteoblast, and " bone loss disease " Therefore, the present invention includes all diseases which are caused by the fact that the activity of osteoclast is excessively increased, bone loss is lowered, bone density is lowered, osteoblast activity is lowered, and bone formation does not occur smoothly.
  • Specific examples of the bone loss diseases include, but are not limited to, osteoporosis, Paget's disease, alveolar bone loss, osteomalacia and renal osteodystrophy.
  • osteoporosis is one of the symptoms of menopausal or menopausal symptoms, which may be caused by reduction of female hormones, or by destruction of osteoblast or inhibition of activity.
  • menopausal period &quot generally refers to a period of transition from a period of reproductive ability to a period of reproductive failure.
  • the menopausal period is mainly used to refer to women's menopause, but women's menopause is not limited to menopause (menopause), including the period before and after the perimenopause (perimenopause) is generally included in the age range of 40 to 60 years.
  • composition comprising CHP or prostate extract of the present invention can prevent, ameliorate or treat bone loss diseases by promoting osteoblast differentiation and promoting bone formation.
  • the term " prevention " means any action that inhibits or delays the onset of a disease or condition. In the present invention, it means that osteoclast differentiation is promoted to delay the onset of osteoporotic disease or suppress the onset.
  • the term " improvement " means any action that improves or alleviates a disease or pathological condition.
  • a disease or pathological condition which is a symptom of osteoporosis or alveolar bone loss.
  • the term " treatment " means all actions that delay, stop, or reverse the progress of disease or pathology.
  • CHP alone was administered to an ovariectomized animal model, and as a result, the bone mineral density and bone volume ratio of the femur were significantly increased as shown in FIGS. 1 and 2.
  • the effect of CHP was remarkably better than that of mixed administration group of zinc salt administration group and zinc salt and CHP combination, thereby increasing the bone density through the effect of increasing the absorption rate of zinc in the body known as CHP It can be inferred that it is not.
  • CHP may be administered at a dose of 0.1 to 5 mg / L, preferably 0.5 to 3 mg / L.
  • &quot pharmaceutically acceptable " refers to those which are physiologically acceptable and which, when administered to humans, do not normally cause an allergic reaction or a similar reaction, wherein the salt includes a pharmaceutically acceptable free acid acid is preferred.
  • the pharmaceutically acceptable salt may be an acid addition salt formed using an organic acid or an inorganic acid.
  • the organic acid may be, for example, formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, malic acid, But are not limited to, malonic acid, fumaric acid, succinic acid, succinic monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, dichloroacetic acid, aminooxyacetic acid, Toluenesulfonic acid or methanesulfonic acid.
  • the inorganic acid includes, for example, hydrochloric acid, bromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid or boric acid.
  • the acid addition salt may preferably be in the form of a hydrochloride or an acetate, more preferably in the form of a hydrochloride.
  • forms which may additionally be salified include, but are not limited to, the salts of gabapentin, gabapentin, pregabalin, nicotinate, adipate, hemimarate, cysteine salt, acetylcysteine salt, methionine salt, arginine salt, lysine salt, ornithine salt or aspartate .
  • the pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier.
  • the pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration.
  • Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like.
  • the carrier for parenteral administration may contain water, a suitable oil, saline, aqueous glucose and glycol, and the like.
  • stabilizers and preservatives may be further included. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid.
  • Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol.
  • Other pharmaceutically acceptable carriers can be found in Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, Pa., 1995).
  • the pharmaceutical composition of the present invention can be administered to mammals including humans by any method.
  • it can be administered orally or parenterally, and parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, , Intranasal, enteral, topical, sublingual or rectal administration.
  • the pharmaceutical composition of the present invention can be formulated into oral preparations or parenteral administration preparations according to the administration route as described above.
  • one or more buffers e. G., Saline or PBS
  • carbohydrates e. G., Glucose, mannose, sucrose or dextran
  • antioxidants e. G., Glucose, mannose, sucrose or dextran
  • bacteriostats e.g., chelating agents Or glutathione
  • fillers e. G., Glucose, mannose, sucrose or dextran
  • bacteriostats e.g., chelating agents Or glutathione
  • fillers e. G., Glucose, mannose, sucrose or dextran
  • bacteriostats e. G., Glucose, mannose, sucrose or dextran
  • bacteriostats e.g., chelating agents Or glutathione
  • fillers e. G., Glu
  • Solid preparations for oral administration include tablets, pills, powders, granules, solutions, gels, syrups, slurries, suspensions or capsules, etc. These solid preparations can be prepared by incorporating into the pharmaceutical composition of the present invention at least one excipient, , Starch (including corn starch, wheat starch, rice starch and potato starch), calcium carbonate, sucrose, lactose, dextrose, sorbitol, mannitol, xylitol, erythritol maltitol, , Methyl cellulose, sodium carboxymethyl cellulose and hydroxypropylmethyl-cellulose or gelatin.
  • tablets or tablets may be obtained by combining the active ingredient with a solid excipient, then milling it, adding suitable auxiliaries, and processing the mixture into granules.
  • lubricants such as magnesium stearate talc are also used.
  • liquid preparation for oral use include suspensions, solutions, emulsions or syrups.
  • various excipients such as wetting agents, sweeteners, fragrances or preservatives may be included .
  • crosslinked polyvinylpyrrolidone, agar, alginic acid, or sodium alginate may optionally be added as a disintegrant, and may further include an anticoagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent .
  • the pharmaceutical compositions of the present invention may be formulated in accordance with methods known in the art in the form of injectable, transdermal and nasal inhalers, together with suitable non-oral carriers.
  • suitable non-oral carriers include, but are not limited to, solvents or dispersion media containing water, ethanol, polyols (e.g., glycerol, propylene glycol and liquid polyethylene glycol, etc.), mixtures thereof and / or vegetable oils .
  • suitable carriers include isotonic solutions such as Hanks' solution, Ringer's solution, phosphate buffered saline (PBS) containing triethanolamine, or sterile water for injection, 10% ethanol, 40% propylene glycol and 5% dextrose Etc. may be used.
  • isotonic solutions such as Hanks' solution, Ringer's solution, phosphate buffered saline (PBS) containing triethanolamine, or sterile water for injection, 10% ethanol, 40% propylene glycol and 5% dextrose Etc.
  • PBS phosphate buffered saline
  • sterile water for injection 10% ethanol
  • propylene glycol 5% dextrose Etc.
  • various antibacterial and antifungal agents such as parabens, chlorobutanol, phenol, sorbic acid, thimerosal and the like may be further included.
  • the injections may in most cases additionally include isotonic agents, such as sugars or sodium chloride
  • transdermal dosage forms include ointments, creams, lotions, gels, solutions for external use, pastes, liniments, and air lozenges.
  • transdermal administration &quot it is meant that the pharmaceutical composition is locally administered to the skin, whereby an effective amount of the active ingredient contained in the pharmaceutical composition is delivered into the skin.
  • the compounds used according to the invention can be formulated into a pressurized pack or a pressurized pack using a suitable propellant, for example dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gases. It can be conveniently delivered in the form of an aerosol spray from a nebulizer.
  • the dosage unit may be determined by providing a valve that delivers a metered amount.
  • gelatin capsules and cartridges used in inhalers or insufflators may be formulated to contain the compound and a powder mixture of suitable powder base such as lactose or starch. Formulations for parenteral administration are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, commonly known in all pharmaceutical chemistries.
  • the pharmaceutical composition of the present invention can provide a preventive, ameliorative or therapeutic effect of a bone loss disease which is preferable when the effective amount of the cyclo-heaps or prostate extract is contained.
  • the term " effective amount &quot refers to an amount that exhibits a greater response than a negative control, preferably an amount sufficient to prevent, ameliorate, or treat a bone loss disease.
  • the pharmaceutical composition of the present invention may contain 0.01 to 99.9% of cyclohexane or prostate extract, and the remaining amount may be occupied by a pharmaceutically acceptable carrier.
  • the effective amount of the cyclo-hepto or prostate extract contained in the pharmaceutical composition of the present invention will vary depending on the form and the like of the composition.
  • the total effective amount of the pharmaceutical composition of the present invention may be administered to a patient in a single dose and may be administered by a fractionated treatment protocol administered over a prolonged period of time in multiple doses .
  • the pharmaceutical composition of the present invention may vary in the content of the active ingredient depending on the degree of the disease. For example, it may be administered in one to several divided doses so as to be preferably administered in an amount of 0.001 to 100 mg, more preferably 0.01 to 10 mg per kg of body weight per day on the basis of cyclo-heaps.
  • the dose of the cyclohexane or prostatic extract is not limited to the administration route and the number of treatments of the pharmaceutical composition, but also to various factors such as the patient's age, weight, health, sex, severity of disease, A person skilled in the art will recognize that the cyclohexane or prostate extract can be used for the prevention, treatment or amelioration of a bone loss disease by appropriate effective administration according to the specific use It will be possible to determine the amount.
  • the pharmaceutical composition according to the present invention is not particularly limited to the formulation, administration route and administration method as long as the effect of the present invention is exhibited.
  • the pharmaceutical composition for preventing or treating bone loss diseases of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy or biological response modifiers.
  • the pharmaceutical composition for preventing or treating bone loss diseases of the present invention may also be provided as a formulation of a topical composition containing a cyclo-heptauro or prostate extract.
  • the composition of the present invention may be a quasi-drug composition for prevention or improvement of bone loss diseases and a quasi-drug comprising the composition.
  • the external agent may be applied directly to the skin or oral cavity.
  • the pharmaceutical composition for preventing or treating a bone loss disease of the present invention may further contain a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic emulsifiers, nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic active agents, lipophilic active agents Or any other ingredient conventionally used in skin topical agents such as lipid vesicles.
  • the components can be introduced in amounts commonly used in the dermatology field.
  • the composition of the present invention when provided as a external preparation, it may be a formulation such as, but not limited to, a liquid, an ointment, a patch, a gel, a cream or a spray.
  • the external medicine of the present invention may include an oral care product including toothpaste, a mouthwash and a mouse spray, an ointment, a mask, a poultice, a patch, and a transdermal absorbent.
  • the quasi-drug composition may be a composition for oral care for preventing or improving bone loss.
  • the cyclohexane or prostate extract may be directly added, or may be appropriately used according to a conventional method together with other quasi-drugs.
  • the amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).
  • the contents of the pharmaceutical composition and the health functional food composition of the present invention can be applied mutatis mutandis to the quasi-drug composition and quasi-drug of the present invention.
  • the term " health functional food &quot includes both the meaning of " functional food " and " health food ".
  • the term " functional food &quot is the same term as a food for special health use (FoSHU).
  • the term " functional food " It means high food.
  • the term " health food " refers to a food having an active health promotion or promotion effect as compared with a general food, and a health supplement food means a food for health assistance.
  • a health supplement food means a food for health assistance.
  • terms of functional foods, health foods, and health supplements are mixed.
  • the food may be prepared in various forms such as tablets, capsules, powders, granules, liquids, rings and the like in order to obtain a beneficial effect for improvement or recovery of bone loss diseases.
  • the health functional food composition of the present invention may also be manufactured in the form of nutritional supplement, food additives, feed, etc., and animals, including human beings or livestock, are taken for food.
  • Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art.
  • Common foods include but are not limited to beverages (including alcoholic beverages), fruits and processed foods (eg, canned fruits, jam, maamalade, etc.), fish, meat and processed foods (Eg butter, chewing), edible vegetable oil, margarine (such as corn oil, etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), juice, various drinks, cookies, , A vegetable protein, a retort food, a frozen food, various kinds of seasonings (for example, soybean paste, soy sauce, sauce, etc.).
  • the nutritional supplement may be prepared by adding a cyclo-hethrope or prostate extract to a capsule, a tablet or a ring.
  • the health functional foods include, but are not limited to, for example, liquefying, granulating, encapsulating and pulverizing such that the above-mentioned cyclo-heaps or prostate extract is prepared in the form of tea, juice and drink, And can be ingested.
  • the above-mentioned cyclo-heath or prostate extract in the form of a food additive, it may be used in the form of a powder or a concentrate.
  • the composition may be prepared by mixing the above-mentioned cyclo-hippro or prostate extract with known active ingredients known to be effective for preventing or improving bone loss diseases.
  • the health beverage composition may contain various flavors or natural carbohydrates as additional components such as ordinary beverages.
  • the above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose, sucrose; Polysaccharides such as dextrin, cyclodextrin; Xylitol, sorbitol, erythritol, and the like.
  • Sweeteners include natural sweeteners such as tau Martin and stevia extract; Synthetic sweetening agents such as saccharin and aspartame, and the like can be used.
  • the ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.
  • the cyclo-hspro or prostate extract may be contained as an active ingredient of a food composition for preventing or ameliorating bone loss diseases, the amount of which is effective for obtaining the above-mentioned preventive or improving effect, for example, It is preferably 0.01 to 100% by weight, but is not particularly limited thereto.
  • the food composition of the present invention may be prepared by mixing together with other active ingredients known to be effective in preventing or ameliorating bone loss diseases along with cyclo-hepta or prostate extract.
  • the health functional food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acid, protective colloid thickener, pH adjuster, , Glycerin, an alcohol or a carbonating agent, and the like.
  • the health food of the present invention may contain natural fruit juice, fruit juice drink, or pulp for the production of vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
  • the zinc salts used in the following examples were purchased from Captek Softgel International, and CHP (cyclo-heptane) was purchased from Bachem.
  • the hPTH 1 -34 as positive control group (PTH 1-34) was purchased from less torque.
  • Gibco's MEM ⁇ , no ascorbic acid medium and hybrone FBS were purchased and ⁇ -glycerophosphate and ascorbic acid used for differentiation were purchased from Sigma-Aldrich Respectively.
  • ALP alkaline phosphatase
  • Thermo's 1-STEP TM NBCT / BCIP solution was used.
  • Example 1 Measuring effect of improving osteoporosis using ovariectomized animal model by CHP or zinc salt and mixed administration
  • Bone mineral density (BMD) of the ovariectomized animals was measured by micro CT (QuantumFX, Perkin Elmer, Massachusetts, USA). Bone volume fraction (BV / TV,%) was also measured. The results of measuring the bone mineral density of the femur are shown in Fig. 1, and the bone volume ratio is shown in Fig.
  • MC3T3-E1 cells were cultured in an incubator under the conditions of 37 ° C and 5% CO 2 using MEM ⁇ and ascorbic acid-free medium containing 10% FBS and 1% penicillin / streptomycin.
  • 10 mM ⁇ -glycerophosphate and 50 ⁇ g / ⁇ l ascorbic acid were added to the basic medium composition.
  • the medium On the 14th day of differentiation, the medium was removed, washed once with PBS, and fixed with 4% PFA (paraformaldehyde) for 5 minutes at room temperature. 500 ⁇ l of 1-STEP TM NBCT / BCIP solution was added and reacted at room temperature for 1 hour. After washing with distilled water, the cells were dried at room temperature and the degree of staining of the cells was observed under a microscope. DMSO was then added to each well, and the absorbance at 525 nm was measured after shaking.
  • PFA paraformaldehyde

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PCT/KR2018/014275 2017-11-20 2018-11-20 Chp(시클로-히스프로)를 포함하는 골 손실 질환의 예방, 개선 또는 치료용 조성물 Ceased WO2019098811A2 (ko)

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EP18879826.8A EP3714885B1 (en) 2017-11-20 2018-11-20 Composition for preventing, alleviating, or treating bone loss diseases, comprising cyclo(his-pro) (chp)
CN201880074979.3A CN111372586B (zh) 2017-11-20 2018-11-20 包含chp的骨丢失疾病的预防、改善或治疗用组合物
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KR102575908B1 (ko) * 2020-12-11 2023-09-07 전남대학교산학협력단 락토바실러스 사케이 배양액 유래 사이클로디펩티드계 화합물의 분리방법 및 이를 포함하는 골 질환 예방 또는 치료용 조성물
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