WO2019022538A3 - 소수성 상호작용을 이용한 세포밖 소포체의 분리방법 - Google Patents

소수성 상호작용을 이용한 세포밖 소포체의 분리방법 Download PDF

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Publication number
WO2019022538A3
WO2019022538A3 PCT/KR2018/008479 KR2018008479W WO2019022538A3 WO 2019022538 A3 WO2019022538 A3 WO 2019022538A3 KR 2018008479 W KR2018008479 W KR 2018008479W WO 2019022538 A3 WO2019022538 A3 WO 2019022538A3
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extracellular vesicles
isolating
various
research
tissues
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PCT/KR2018/008479
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English (en)
French (fr)
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WO2019022538A2 (ko
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이창진
고용송
박현택
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(주)로제타엑소좀
포항공과대학교 산학협력단
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Priority to CN201880058719.7A priority Critical patent/CN111065730A/zh
Priority to US16/634,076 priority patent/US20240027312A1/en
Priority to JP2020527718A priority patent/JP7364240B2/ja
Priority to EP18838627.0A priority patent/EP3660143A4/en
Publication of WO2019022538A2 publication Critical patent/WO2019022538A2/ko
Publication of WO2019022538A3 publication Critical patent/WO2019022538A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B01J20/267—Cross-linked polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

본 발명은 세포밖 소포체의 분리 방법에 관한 것으로서, 보다 상세하게 본 발명은 세포밖 소포체의 소수성을 이용하여 세포밖 소포체를 분리하는 방법, 상기 방법을 이용하여 분리된 세포밖 소포체 등에 관한 것이다. 본 발명의 세포밖 소포체 분리 방법을 이용하면 혈액과 소변을 포함한 다양한 동물의 체액 또는 암조직을 포함한 다양한 조직에서 기존의 방법으로는 어려웠던 지질단백질의 오염이 제거된 세포밖 소포체를 분리할 수 있게 되어, 종래 지질단백질 오염으로 야기된 문제를 해결하고, 동물의 다양한 체액 또는 조직에서 분리한 세포밖 소포체를 이용해 특성과 기능 연구, 다중 오믹스 연구, 신규 바이오 마커의 발굴, 진단과 치료 등 다양한 연구에 파급효과가 큰 필수 기술이 될 것으로 기대된다.
PCT/KR2018/008479 2017-07-26 2018-07-26 소수성 상호작용을 이용한 세포밖 소포체의 분리방법 WO2019022538A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN201880058719.7A CN111065730A (zh) 2017-07-26 2018-07-26 利用疏水相互作用分离细胞外囊泡的方法
US16/634,076 US20240027312A1 (en) 2017-07-26 2018-07-26 Method for isolating extracellular vesicle using hydrophobic interaction
JP2020527718A JP7364240B2 (ja) 2017-07-26 2018-07-26 疎水性相互作用を利用した細胞外小胞体の分離方法
EP18838627.0A EP3660143A4 (en) 2017-07-26 2018-07-26 METHOD OF ISOLATION OF EXTRACELLULAR VESICLES USING HYDROPHOBIC INTERACTION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020170094874A KR101999818B1 (ko) 2017-07-26 2017-07-26 소수성 상호작용을 이용한 세포밖 소포체의 분리방법
KR10-2017-0094874 2017-07-26

Publications (2)

Publication Number Publication Date
WO2019022538A2 WO2019022538A2 (ko) 2019-01-31
WO2019022538A3 true WO2019022538A3 (ko) 2019-04-11

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PCT/KR2018/008479 WO2019022538A2 (ko) 2017-07-26 2018-07-26 소수성 상호작용을 이용한 세포밖 소포체의 분리방법

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US (1) US20240027312A1 (ko)
EP (1) EP3660143A4 (ko)
JP (1) JP7364240B2 (ko)
KR (1) KR101999818B1 (ko)
CN (1) CN111065730A (ko)
WO (1) WO2019022538A2 (ko)

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CN111621471B (zh) * 2020-06-12 2022-03-11 成都世联康健生物科技有限公司 一种软组织胞外囊泡的提取方法及应用
CN111961637A (zh) * 2020-07-08 2020-11-20 暨南大学 一种基于尺寸排阻层析与超滤结合的细胞外囊泡分离方法
WO2022150818A1 (en) * 2021-01-06 2022-07-14 Yuvan Research, Inc. Anti-aging compositions and uses thereof
CN113092642B (zh) * 2021-03-30 2022-11-01 苏州爱宝德生物科技有限公司 一种用于细胞外囊泡的快速提取装置
CN118103495A (zh) * 2021-06-04 2024-05-28 基础科学研究院 使用盐分级沉淀分离细胞外囊泡的方法
CN115521894A (zh) * 2021-06-24 2022-12-27 上海思路迪生物医学科技有限公司 用于提取细胞外囊泡的试剂盒与方法
CN114570152B (zh) * 2022-03-28 2023-12-19 南京诺令生物科技有限公司 一种分离与提纯低颗粒物气体的集成装置及其分离提纯方法
CN116687966A (zh) * 2023-07-05 2023-09-05 青岛青薄荷生物科技有限公司 一种治疗脚气的药剂、分离方法及盛纳装置

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Also Published As

Publication number Publication date
JP7364240B2 (ja) 2023-10-18
EP3660143A2 (en) 2020-06-03
JP2020530780A (ja) 2020-10-29
EP3660143A4 (en) 2021-04-28
CN111065730A (zh) 2020-04-24
WO2019022538A2 (ko) 2019-01-31
US20240027312A1 (en) 2024-01-25
KR101999818B1 (ko) 2019-07-12
KR20190012022A (ko) 2019-02-08

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