WO2019022538A3 - 소수성 상호작용을 이용한 세포밖 소포체의 분리방법 - Google Patents
소수성 상호작용을 이용한 세포밖 소포체의 분리방법 Download PDFInfo
- Publication number
- WO2019022538A3 WO2019022538A3 PCT/KR2018/008479 KR2018008479W WO2019022538A3 WO 2019022538 A3 WO2019022538 A3 WO 2019022538A3 KR 2018008479 W KR2018008479 W KR 2018008479W WO 2019022538 A3 WO2019022538 A3 WO 2019022538A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extracellular vesicles
- isolating
- various
- research
- tissues
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000002209 hydrophobic effect Effects 0.000 title 1
- 230000003993 interaction Effects 0.000 title 1
- 102000004895 Lipoproteins Human genes 0.000 abstract 2
- 108090001030 Lipoproteins Proteins 0.000 abstract 2
- 210000001124 body fluid Anatomy 0.000 abstract 2
- 239000010839 body fluid Substances 0.000 abstract 2
- 238000011109 contamination Methods 0.000 abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 238000009412 basement excavation Methods 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 239000000101 novel biomarker Substances 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/287—Non-polar phases; Reversed phases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
본 발명은 세포밖 소포체의 분리 방법에 관한 것으로서, 보다 상세하게 본 발명은 세포밖 소포체의 소수성을 이용하여 세포밖 소포체를 분리하는 방법, 상기 방법을 이용하여 분리된 세포밖 소포체 등에 관한 것이다. 본 발명의 세포밖 소포체 분리 방법을 이용하면 혈액과 소변을 포함한 다양한 동물의 체액 또는 암조직을 포함한 다양한 조직에서 기존의 방법으로는 어려웠던 지질단백질의 오염이 제거된 세포밖 소포체를 분리할 수 있게 되어, 종래 지질단백질 오염으로 야기된 문제를 해결하고, 동물의 다양한 체액 또는 조직에서 분리한 세포밖 소포체를 이용해 특성과 기능 연구, 다중 오믹스 연구, 신규 바이오 마커의 발굴, 진단과 치료 등 다양한 연구에 파급효과가 큰 필수 기술이 될 것으로 기대된다.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201880058719.7A CN111065730A (zh) | 2017-07-26 | 2018-07-26 | 利用疏水相互作用分离细胞外囊泡的方法 |
US16/634,076 US20240027312A1 (en) | 2017-07-26 | 2018-07-26 | Method for isolating extracellular vesicle using hydrophobic interaction |
JP2020527718A JP7364240B2 (ja) | 2017-07-26 | 2018-07-26 | 疎水性相互作用を利用した細胞外小胞体の分離方法 |
EP18838627.0A EP3660143A4 (en) | 2017-07-26 | 2018-07-26 | METHOD OF ISOLATION OF EXTRACELLULAR VESICLES USING HYDROPHOBIC INTERACTION |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020170094874A KR101999818B1 (ko) | 2017-07-26 | 2017-07-26 | 소수성 상호작용을 이용한 세포밖 소포체의 분리방법 |
KR10-2017-0094874 | 2017-07-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2019022538A2 WO2019022538A2 (ko) | 2019-01-31 |
WO2019022538A3 true WO2019022538A3 (ko) | 2019-04-11 |
Family
ID=65040826
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2018/008479 WO2019022538A2 (ko) | 2017-07-26 | 2018-07-26 | 소수성 상호작용을 이용한 세포밖 소포체의 분리방법 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20240027312A1 (ko) |
EP (1) | EP3660143A4 (ko) |
JP (1) | JP7364240B2 (ko) |
KR (1) | KR101999818B1 (ko) |
CN (1) | CN111065730A (ko) |
WO (1) | WO2019022538A2 (ko) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111621471B (zh) * | 2020-06-12 | 2022-03-11 | 成都世联康健生物科技有限公司 | 一种软组织胞外囊泡的提取方法及应用 |
CN111961637A (zh) * | 2020-07-08 | 2020-11-20 | 暨南大学 | 一种基于尺寸排阻层析与超滤结合的细胞外囊泡分离方法 |
WO2022150818A1 (en) * | 2021-01-06 | 2022-07-14 | Yuvan Research, Inc. | Anti-aging compositions and uses thereof |
CN113092642B (zh) * | 2021-03-30 | 2022-11-01 | 苏州爱宝德生物科技有限公司 | 一种用于细胞外囊泡的快速提取装置 |
CN118103495A (zh) * | 2021-06-04 | 2024-05-28 | 基础科学研究院 | 使用盐分级沉淀分离细胞外囊泡的方法 |
CN115521894A (zh) * | 2021-06-24 | 2022-12-27 | 上海思路迪生物医学科技有限公司 | 用于提取细胞外囊泡的试剂盒与方法 |
CN114570152B (zh) * | 2022-03-28 | 2023-12-19 | 南京诺令生物科技有限公司 | 一种分离与提纯低颗粒物气体的集成装置及其分离提纯方法 |
CN116687966A (zh) * | 2023-07-05 | 2023-09-05 | 青岛青薄荷生物科技有限公司 | 一种治疗脚气的药剂、分离方法及盛纳装置 |
Citations (4)
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WO2012087241A1 (en) * | 2010-12-20 | 2012-06-28 | Agency For Science, Technology And Research | Method of purifying exosomes |
KR20130020789A (ko) * | 2010-05-19 | 2013-02-28 | 에프. 호프만-라 로슈 아게 | 소수성 상호작용 크로마토그래피 방법 |
KR20130069515A (ko) * | 2011-12-15 | 2013-06-26 | 한화케미칼 주식회사 | 항체의 정제 방법 |
KR20160116802A (ko) * | 2015-03-31 | 2016-10-10 | 포항공과대학교 산학협력단 | 수용액 이상계를 이용한 세포 밖 소포체의 분리방법 |
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SE413986B (sv) * | 1973-03-23 | 1980-07-07 | Exploaterings Ab Tbf | Sett att separera amfipatiska emnen innehallande bade hydrofila och hydrofoba grupper samt gelprodukt for genomforande av separationen |
JPS62186940A (ja) * | 1986-02-10 | 1987-08-15 | Kanegafuchi Chem Ind Co Ltd | リポ蛋白用吸着体 |
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2017
- 2017-07-26 KR KR1020170094874A patent/KR101999818B1/ko active IP Right Grant
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2018
- 2018-07-26 CN CN201880058719.7A patent/CN111065730A/zh active Pending
- 2018-07-26 JP JP2020527718A patent/JP7364240B2/ja active Active
- 2018-07-26 EP EP18838627.0A patent/EP3660143A4/en active Pending
- 2018-07-26 WO PCT/KR2018/008479 patent/WO2019022538A2/ko unknown
- 2018-07-26 US US16/634,076 patent/US20240027312A1/en active Pending
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WO2012087241A1 (en) * | 2010-12-20 | 2012-06-28 | Agency For Science, Technology And Research | Method of purifying exosomes |
KR20130069515A (ko) * | 2011-12-15 | 2013-06-26 | 한화케미칼 주식회사 | 항체의 정제 방법 |
KR20160116802A (ko) * | 2015-03-31 | 2016-10-10 | 포항공과대학교 산학협력단 | 수용액 이상계를 이용한 세포 밖 소포체의 분리방법 |
Non-Patent Citations (1)
Title |
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CHEN, TAO: "Hydrophobic Interaction Chromatography for Antibody Drug Conjugate Drug Distribution Analysis", 2015, pages 1 - 6, XP055673464, Retrieved from the Internet <URL:https://www.americanpharmaceuticalreview.com/Featured-Articles/177927-Hydrophobic-Interaction-Chromatography-for-Antibody-Drug-Conjugate-Drug-Distribution-Analysis/> * |
Also Published As
Publication number | Publication date |
---|---|
JP7364240B2 (ja) | 2023-10-18 |
EP3660143A2 (en) | 2020-06-03 |
JP2020530780A (ja) | 2020-10-29 |
EP3660143A4 (en) | 2021-04-28 |
CN111065730A (zh) | 2020-04-24 |
WO2019022538A2 (ko) | 2019-01-31 |
US20240027312A1 (en) | 2024-01-25 |
KR101999818B1 (ko) | 2019-07-12 |
KR20190012022A (ko) | 2019-02-08 |
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