WO2019019416A1 - Medical fertilizer containing plant tissue extract and preparation method therefor - Google Patents

Abstract

Disclosed is a medical fertilizer containing a plant tissue extract. The medical fertilizer contains a plant tissue extract A and a fertilizer B, wherein the plant tissue extract A is extracted from a mixture of rape inflorescence, turmeric root tuber, the whole herb of Aconitum apetalum (Huth) B. Fedtsch., and garlic bulb; the fertilizer B is selected from a ternary compound/mixed fertilizer containing nitrogen, phosphorus and potassium, and the total nitrogen (N) accounts for 15% of the fertilizer mass fraction, the total phosphorus (P2O5) for 15% of the fertilizer mass fraction, and the total potassium (K2O) for 15% of the fertilizer mass fraction; and the mixed application of the plant tissue extract A and the fertilizer B has a synergic effect on the prevention and treatment of potato black scurf. The medical fertilizer makes use of resources effectively, scientifically and rationally, has an important significance for protecting the environment and plays a positive promotion role in reducing agricultural input and increasing production and income in agriculture.

Description

Medicine fertilizer containing plant tissue extract and preparation method thereof Technical field

The invention relates to a medicine fertilizer, in particular to a medicine fertilizer containing plant tissue extract and a preparation method thereof.

Background technique

Potato black shank, also known as Rhizoctonia solani, stem-based rot, Rhizoctonia ulcer, black rough skin disease, is a soil-borne disease, infecting potatoes by Rhizoctonia solani Caused by wintering in the tubers and soil, it can survive for 2 to 3 years in the soil. In the spring of the next year, under the appropriate temperature and humidity conditions, the invading potato plants endangers its growth and development. The disease has been There have been different degrees of occurrence in the area.

In addition to infecting potatoes, Rhizoctonia solani causes potato sheath blight, infecting rice to cause rice sheath blight, infesting peanuts to cause peanut white rot, and infesting corn to cause corn sheath blight.

Pesticides play an important role in agricultural production. China is a big agricultural country. Among them, pesticide residues exceeding the standard are often encountered problems, which seriously affect the competitiveness of China's agricultural products in the international market.

Traditional pesticides are mostly chemical preparations. How to use plant sources as pesticide components to reduce pollution is a technical problem that needs to be solved. Therefore, it is necessary to have a simple ratio and convenient use, and to prepare a plant-derived pesticide with a small degree of pollution by using plant-derived materials as the main material.

In the traditional agricultural production technology, the application of the fertilizer and the above-mentioned fungicide are separate, and the use of the above-mentioned fungicides and fertilizers twice or more increases the labor intensity and labor times of the farmers, which is disadvantageous to agricultural development. The application of the drug granules at one time can complete the two processes of fertilization and control of blight at the same time, avoiding the situation that the previous application and fertilization cannot be taken into consideration, saving time and labor, and improving production efficiency.

At present, it contains extracts from rapeseed, turmeric roots, aconite whole grass, garlic bulb mixture; fertilizer mixed with fertilizer has not been reported.

Rapeseed, also known as oil cabbage, bitter herbs, Latin name Brassica campestris L. Cruciferae, Brassica, tender stems and leaves of rapeseed, native to China, its stem color dark green, help for cabbage, is a cross flower Cabbage change Species, the flowers are yellow; agronomically, the various species of oil in the plants are collectively referred to as rapeseed. At present, the main types of cultivation (variety) of rapeseed are: Brassica rapa (campestris L.), Brassica juncea (Brassica juncea) L.), Brassica napus L.; Brassinolide in Brassica napus L.

Turmeric, Latin name Curcuma longa L., also known as: turmeric, Bao Dingxiang, milli-life, turmeric, etc.; Zingiberaceae, turmeric is a perennial herb, plant height 1 ~ 1.5m, the roots are very developed, the roots are thick, The end is swollen into a lump, the leaves are oblong or elliptical, the tip of the leaf is short and abrupt, the sepals are ovate or oblong, pale green, apical, corolla yellow, flowering in August; After pain relief, attending chest and abdomen pain, shoulder and arm pain, heart pain, postpartum blood pain, early onset of sores, irregular menstruation, amenorrhea, bruises.

Aconite, abbreviated as Aconitum apetalum, herbaceous plant of the genus Aconitum, with thick stems, up to 2 m, up to 1.5 cm thick, basal leaves rounded in kidney shape; racemes, most densely flowering, 苞The line is linear, the bracts are white or pale yellow, the upper bracts are high-helmet-shaped, the petals are shorter than the bracts, the filaments are entire, the carpels are 3, the ovary is short-haired; the altitude is between 1700 and 1900 meters, under the mountain spruce forest or the valley grass slope , distributed in Xinjiang, China, Gongliu and Kazakhstan.

Garlic, a Latin name called Allium sativum, belongs to the genus Allium, and is bulbous. The garlic bulb is spheroidal or short conical. It has grayish white or light brown membranous scales on the outside. Stripped scales are included. Garlic cloves, which are born around the stems, have a disc-shaped base, and have many fibrous roots. Each garlic clove is covered with a film, and the film is peeled off. It is white, thick and juicy, with strong garlic and spicy taste.

Compound and mixed fertilizers are two kinds of fertilizers (three elements (nitrogen, phosphorus, potassium)), two kinds of compound fertilizers, and three-component complex fertilizers containing nitrogen, phosphorus and potassium. It is widely promoted throughout the country. Total nitrogen in complex and mixed fertilizers is calculated by N, total phosphorus is calculated by P ₂ O ₅ , and total potassium is calculated by K ₂ O.

Summary of the invention

The invention provides a medicine fertilizer which is scientifically combined with a carrier fungicide and a carrier fertilizer. The agricultural application can simultaneously complete two processes of sterilization and fertilization in one application, and overcomes the user twice under the prior art conditions. Or the use of fungicides and fertilizers multiple times, saving time and labor and increasing production efficiency.

Technical Solution: A fertilizer containing plant tissue extract, the plant fertilizer containing plant tissue extract A and fertilizer B; wherein the plant tissue extract A is extracted from rapeseed, turmeric root, empty stem aconite whole grass, garlic bulb a mixture; wherein the fertilizer B is selected from the group consisting of three-way complex and mixed fertilizer containing nitrogen, phosphorus and potassium, the total nitrogen (N) is 15% by mass of the fertilizer, and the total phosphorus (P ₂ O ₅ ) is the percentage by mass of the fertilizer. At 15%, total potassium (K ₂ O) accounts for 15% of the fertilizer mass fraction; plant tissue extract A and fertilizer B have a synergistic effect on potato black rot disease; among them, preferred plant tissue extract A and fertilizer B The mass fraction ratio is 1:200 to 500, and more preferably the ratio of the mass fraction of the plant tissue extract A to the fertilizer B is 1:400.

The plant tissue extract A, wherein the ratio of the mass fraction of the rape inflorescence, the turmeric root, the aconite whole grass, and the garlic bulb is 1:2:2:15.

The extraction method of the plant tissue extract A is as follows: 10 parts of cauliflower, 20 parts of turmeric roots, 20 parts of black stalks, and 120 parts of garlic bulbs, which are respectively mashed and mixed together by a tissue masher. 10 parts of EO-PO block polymer, 10 parts of epoxy rapeseed oil, 10 parts of methyl oleate, 270 parts of deionized water, and stirred for 30 minutes in a blender, the mixed liquid was centrifuged at 5,000 rpm. After 20 minutes, the supernatant was taken to prepare the plant tissue extract A.

The fertilizer B is characterized in that: total nitrogen (N) accounts for 15% of the mass of the fertilizer, and total phosphorus (P ₂ O ₅ ) accounts for 15% of the mass of the fertilizer, and the total potassium (K ₂ O) ) The percentage of fertilizer content is 15%.

The medicine fertilizer containing the plant tissue extract is prepared as follows: several parts of the plant tissue extract A, several parts of the fertilizer B, A are sprayed onto the B by a spray device, uniformly distributed in the B, and dried by the drying equipment. Preparing the plant fertilizer containing the plant tissue extract after drying; the drying means that the mass fraction of water in the fertilizer accounts for less than 0.5% of the total mass fraction of the fertilizer; the drying equipment is baked The dry temperature is 40 ± 1 degree Celsius.

The invention has the following beneficial effects:

The mixed use of the plant tissue extract A and the fertilizer B has a synergistic effect on the control of potato black shank.

The invention comprises plant tissue extracts A and B, wherein A is extracted from a rape inflorescence, turmeric root, aconite whole grass, garlic bulb mixture, wherein the main raw materials of A are derived from plants, plant tissue extract A is opposite to the dead silk The mycelial growth of nuclear bacteria has an inhibitory effect and can be used to control potato black shank disease. The effective, scientific and rational use of resources of the invention has important significance for protecting the environment, and plays a positive role in reducing agricultural input and increasing production and income of agriculture.

Detailed ways

The present invention has been described in terms of the following specific embodiments, but the invention is not limited to these examples. The following description is only a preferred implementation of the present invention. The examples are only intended to describe the invention and are not to be construed as limiting the scope of the invention. It should be noted that any modifications, equivalent substitutions and improvements made within the spirit and scope of the invention are intended to be included within the scope of the invention.

The content ratios described in the examples are all parts by mass.

Example 1: Method for extracting plant tissue extract A.

10 servings of cauliflower, 20 parts of turmeric roots, 20 parts of stalked aconite, 150 parts of garlic bulbs, mashed together with tissue masher, and mixed together, 10 parts of EO-PO block polymer, epoxy 10 parts of rapeseed oil, 10 parts of methyl oleate, 270 parts of deionized water, stirred for 30 minutes in a blender, and the mixed liquid was centrifuged in a centrifuge of 5000 rpm for 20 minutes, and then the supernatant was obtained. The plant tissue extract A. The ratio of the mass fraction of rapeseed, turmeric root, stalked aconite and garlic bulb is 1:2:2:15.

Example 2: Fertilizer B.

Fertilizer B is a compound of nitrogen, phosphorus and potassium, which is prepared by conventional techniques. Among them, total nitrogen (N) accounts for 15% of the mass of fertilizer, and total phosphorus (P ₂ O ₅ ) accounts for the mass of fertilizer. The percentage is 15%, the total potassium (K ₂ O) is 15% of the fertilizer content; the preparation method of fertilizer B is as follows: 100 parts of potassium nitrate, 70 parts of urea, 102 parts of heavy superphosphate, 28 parts of talc The mixture is uniformly mixed, granulated by a drum, and sieved to retain particles having a diameter of 1.8 to 2.0 mm, that is, the fertilizer B is obtained.

Embodiment 3: A method for preparing the above-mentioned medicine fertilizer containing plant tissue extract.

The medicine fertilizer containing the plant tissue extract is prepared as follows: a plurality of the first embodiment, the plant tissue extract A, and a plurality of the second embodiment, the fertilizer B, A are sprayed onto the B through a spraying device, and uniformly distributed in the B, After drying by the drying device, the plant fertilizer containing the plant tissue extract is prepared; the drying means that the mass fraction of water in the drug fertilizer accounts for less than 0.5% of the total mass fraction of the drug fertilizer; The drying temperature of the drying device is 40±1 degrees Celsius.

Table 1 The mixture of A and B prepared by the method described in the third embodiment was prepared to obtain the plant fertilizer containing the plant tissue extract.

Fertilizer number	Ratio of A to B	A number of copies	B number of copies
Medicine fertilizer No. 1	1:100	2	200
Medicine fertilizer 2	1:200	1	200

Medicine fertilizer No. 3	1:300	1	300
Medicine fertilizer 4	1:400	1	400
Medicine fertilizer No. 5	1:500	1	500
Medicine fertilizer 6	1:700	1	700

Example 4: Determination of the activity of the plant tissue extract A against Rhizoctonia solani according to Example 1.

Table 2 Results of indoor activity assay of Rhizoctonia solani of plant tissue extract according to Example 1 (Inhibition of pathogenic fungal mycelial growth test - plate method, refer to NY/T 1156.2-2006, 3 days after inoculation) ).

Serial number (processing)	Treatment concentration (mg/kg)	Mycelial growth inhibition rate (%)
1	5	15.5
2	10	30.6
3	20	43.4
4	40	67.6
5	80	86.4
6	160	100.0

Example 5: The plant tissue extract A of the first embodiment and the fertilizer B of the second embodiment are used alone and mixed in a certain ratio (the pharmaceutical fertilizer No. 1, the drug fertilizer No. 2, the drug fertilizer No. 3 described in the third embodiment) , medicine fertilizer No. 4, medicine fertilizer No. 5, medicine fertilizer No. 6) control effect on potato black shank disease (spraying or spreading when ridged, investigating potato susceptibility during harvest, Table 3, Table 4, Table 5 examples).

Potato potato block black shank grading standard (refer to Ma Yongqiang, Li Jiping published in the "Jiangsu Agricultural Sciences" at the end of 2013, Volume 41, the first issue of the article: two different drugs applied to the comparison of potato black scorpion control effect Disease classification method):

Level 0 - no disease spots on the potato chips;

Level 1 - The area of the diseased part accounts for less than 5% of the entire potato block area (including 5%);

Level 2 - The area of the diseased area accounts for 6%-10% of the total potato area (including 10%);

Level 3 - The area of the diseased area accounts for 11%-25% (including 25%) of the total potato area;

Level 4 - The area of the diseased area accounts for 26%-50% (including 50%) of the total potato area;

Level 5 - The area of the diseased area accounts for 51%-75% (including 75%) of the total potato area;

Level 6 - The area of the diseased area accounts for 76%-100% (including 100%) of the total potato area;

Disease index = ∑ [(number of diseased potatoes at all levels × relative value) ÷ (review total potato number × 6)] × 100

The Colby equation is used to determine the desired effect of the mixture (Colby, S.R. Weeds 15 (1967), 20-22.):

The following equation is used to calculate the expected value of a mixture containing two components, A and B:

In the formula:

A = the efficacy of a certain (dose) concentration of active ingredient A observed;

B = the efficacy of a certain (dose) concentration of active ingredient B observed;

The observed value (E) is the actual observed efficacy;

When the actual observed value (E) of the concentration (dose) activity used in the mixture is greater than the expected value (E ₀ ), the mixture appears to have an unexpected synergistic effect on the target;

For the purposes of the present invention, the observed value (E) is the actual observed effect on the control of potato black shank, and the expected value (E ₀ ) is the desired control effect of potato black shank after mixing A and B at a certain concentration or dose. .

Table 3 The control effect of the different application rates of the plant tissue extract A on the potato black shank in the first embodiment (evenly ridge ridges during ridges, and investigation at the time of harvest). 30 kg of water per acre.

Processing number	Dosage (g/mu)	Control effect (%)
1	20	15.5
2	40	20.3
3	60	30.1
4	80	35.5
5	100	40.2
6	150	51.4
7	200	66.5

Table 4 The effect of different application rates of fertilizer B on the control of potato black shank in Example 2 (spray evenly on the ridge during ridges, and investigate at harvest)

Processing number	Dosage (kg / mu)	Control effect (%)
1	10	0.0
2	20	0.0
3	30	0.0
4	40	0.0
5	50	0.0
6	70	0.0
7	100	0.0

Table 5 The control effect of the combination of the plant tissue extract A and the fertilizer B described in the first embodiment on the potato black shank (the fertilizer B in the second embodiment and the medicinal fertilizer in the third embodiment are evenly ridged) Sprinkle on the ridge, evenly ridged when ridged in the first embodiment, all of which are investigated during harvesting)

The results of the data shown in Table 5 indicate that the ratio of the mass fraction of the fertilizer of the plant tissue extract A and the second embodiment described in the first embodiment is in the range of 1:100-1:700 to the control effect of potato black shank. Synergistic effect, EE _{0 is} greater than 10%; wherein the ratio of A and B mass fractions in the range of 1:200-1:500 has a synergistic effect on the control effect of potato black shank, EE _{0 is} greater than 20%; A and B mass The ratio of the number of parts at 1:400 had the best synergistic effect on the control of potato black shank, and EE _{0 was} greater than 40%.

Example 6: The field efficacy test of the drug fertilizer No. 4 described in the third embodiment for controlling rice sheath blight, corn sheath blight, and peanut chalk disease.

Table 6 The control effect of the drug fertilizer No. 4 on the control of rice sheath blight, corn sheath blight and peanut chalk disease in the third embodiment (administering dose of 50 kg per mu, before or before transplanting, 50 days after the drug) survey).

Claims (4)

1. The invention relates to a medicine fertilizer containing plant tissue extract, which comprises plant tissue extract A and fertilizer B; wherein the plant tissue extract A is extracted from a rape inflorescence, turmeric root, an aconit whole grass, a garlic bulb mixture; The fertilizer B is selected from the group consisting of ternary complex and mixed fertilizer containing nitrogen, phosphorus and potassium;

   The ratio of the mass fraction of the plant tissue extract A and the fertilizer B is 1:200-500;

   The ratio of the mass fraction of the rapeseed, the turmeric root, the aconite whole grass, and the garlic bulb is 1:2:2:15; the fertilizer B, the total nitrogen (N) accounts for 15% of the mass of the fertilizer. Total phosphorus (P ₂ O ₅ ) accounts for 15% of the mass of the fertilizer, and total potassium (K ₂ O) accounts for 15% of the fertilizer.
2. The plant fertilizer extract-containing fertilizer according to claim 1, wherein the plant tissue extract A and the fertilizer B have a mass ratio of 1:400.
3. Method for preparing medicine fertilizer containing plant tissue extract, characterized in that:

   (1) Extraction of plant tissue extract A: 10 parts of rapeseed inflorescence, 20 parts of turmeric roots, 20 parts of empty stalk aconite, 150 parts of garlic bulbs, mashed with tissue masher, mixed together, added 10 parts of EO-PO block polymer, 10 parts of epoxy rapeseed oil, 10 parts of methyl oleate, 270 parts of deionized water, stirred for 30 minutes in a blender, and the mixed liquid was centrifuged at 5,000 rpm. After a minute, the supernatant is taken to prepare the plant tissue extract A;

   (2) Take 10 parts of plant tissue extract A, 4000 parts of fertilizer B, A is sprayed onto B through a spray device, uniformly distributed in B, and dried to obtain the plant tissue extract The drying means that the mass fraction of water in the fertilizer accounts for less than 0.5% of the total mass fraction of the fertilizer; and the drying temperature of the drying equipment is 40±1 degrees Celsius.
4. The use of the plant tissue extract-containing fertilizer according to claim 1 for controlling potato black shank, rice sheath blight, corn sheath blight, and peanut chalk disease.