WO2018060496A1 - Procédé et système permettant de transférer une résine de separation - Google Patents

Procédé et système permettant de transférer une résine de separation Download PDF

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Publication number
WO2018060496A1
WO2018060496A1 PCT/EP2017/074902 EP2017074902W WO2018060496A1 WO 2018060496 A1 WO2018060496 A1 WO 2018060496A1 EP 2017074902 W EP2017074902 W EP 2017074902W WO 2018060496 A1 WO2018060496 A1 WO 2018060496A1
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WO
WIPO (PCT)
Prior art keywords
container
slurry
transferring
vacuum
transferred
Prior art date
Application number
PCT/EP2017/074902
Other languages
English (en)
Inventor
Johan Fredrik AVALLIN
Original Assignee
Ge Healthcare Bio-Sciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1616702.5A external-priority patent/GB201616702D0/en
Priority claimed from GBGB1701576.9A external-priority patent/GB201701576D0/en
Application filed by Ge Healthcare Bio-Sciences Ab filed Critical Ge Healthcare Bio-Sciences Ab
Priority to US16/338,117 priority Critical patent/US10940402B2/en
Priority to CN201780073867.1A priority patent/CN109982764B/zh
Priority to JP2019517004A priority patent/JP6983454B2/ja
Priority to DK17780695.7T priority patent/DK3519072T3/da
Priority to EP17780695.7A priority patent/EP3519072B1/fr
Publication of WO2018060496A1 publication Critical patent/WO2018060496A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/206—Packing or coating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G01N2030/562—Packing methods or coating methods packing
    • G01N2030/565—Packing methods or coating methods packing slurry packing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods

Definitions

  • the present invention relates to a method and a system for transferring a slurry of a separation resin or the like, from at least one first container to a second container.
  • Separation media also called separation resin
  • the media could be for example resins based on natural or synthetic polymer particles or inorganic material.
  • the separation resin needs to be provided into a chromatography column.
  • the separation resin is normally suspended with a liquid, for example water, buffer or a solvent. This suspended resin is usually called resin slurry, media slurry, slurry or slurry of chromatography media/resin.
  • an intermediate slurry tank is often used where the slurry is mixed into a homogenous mixture.
  • the transferring of resin slurry from storage containers to a slurry tank can be handled in different ways. Sometimes the slurry is poured into the slurry tank from the storage containers. However, the containers can be heavy and there may be a risk of spill and waste and biological contamination by operators. Chromatography media is very expensive so waste and contamination need to be avoided.
  • Another method for transferring resin slurry from a storage container to a slurry tank is to use some kind of transfer device, e.g. a membrane pump.
  • the handling and transferring of the slurry is often at least in some steps an open process, i.e. the slurry is exposed to ambient air and transferring means. This can introduce impurities and particles and biological vegetative contamination into the slurry resin.
  • the slurry In the slurry tank the slurry is mixed into a homogenous mixture which then is transferred to the chromatography column. The whole amount of slurry needs to be transferred without interruption in order to avoid that the slurry starts to settle in the chromatography column before the packing procedure begins. When the slurry has been transferred to the column the packing process needs to get started as soon as possible while the slurry still is in suspended, homogenous form in order to achieve a uniformly packed bed.
  • An object of the present invention is to provide an improved method and system for transferring slurry of separation resin between containers.
  • a method for transferring a slurry of a separation resin, from at least one first container to a second container comprising the steps of: - connecting the at least one first container to the second container by a transferring connection;
  • a system for transferring a slurry of a separation resin between at least two containers, said system comprising: - at least one first container which initially comprises a slurry of a separation resin; a second container;
  • At least one vacuum production device connected to the second container and arranged for providing a vacuum in the second container at least during a transferring of the slurry from the at least one first container to the second container;
  • a transferring connection connecting the at least one first container and the second container such that the slurry initially provided in the at least one first container can be transferred from the at least one first container to the second container through the transferring connection by the vacuum provided to the second container.
  • the step of connecting the at least one first container to the second container comprises connecting the two containers by disposable, pre-sterilized connecting parts, i.e. the transferring connection comprises disposable connecting parts, such as pre-sterilized tubing and aseptic connectors.
  • the transferring connection comprises disposable connecting parts, such as pre-sterilized tubing and aseptic connectors.
  • the step of allowing the vacuum in the second container to affect the content in the at least one first container comprises opening a valve provided in the transferring connection between the at least one first container and the second container, i.e. the transferring connection comprises at least one valve whereby opening of the valve allows vacuum provided to the second container to affect the content in the first container such that the slurry initially provided in the first container is transferred to the second container through the transferring connection.
  • opening and closing of the valve controls the transferring of slurry from the first container to the second container.
  • the method further comprises the step of rinsing the at least one first container by transferring a rinsing solution through the at least one first container and further to the second container, said rinsing solution coming from a rinse bag connected to the at least one first container, said transferring of a rinsing solution being accomplished by the same vacuum as provided to the second container for transferring the slurry.
  • the step of rinsing the at least one first container comprises opening a rinsing valve provided in the connection between the at least one first container and the rinse bag to allow the vacuum provided to the second container to transfer rinsing solution from the rinse bag through the at least one first container and finally to the second container and closing the rinse valve when a suitable amount of rinsing solution has been transferred.
  • the method comprises connecting two or more first containers in series or in parallel to the second container allowing the content of all of the first containers to be transferred to the second container through one and the same transferring connection.
  • only one transferring connection needs to be provided for transferring slurry from more than one first containers.
  • risk of contamination is decreased and the handling is facilitated.
  • the first container is a disposable, flexible slurry storage bag and the second container is a slurry tank or a chromatography column.
  • the transferring connection comprises a disposable hollow rod with a first end of the hollow rod arranged for pointing down into the first container and a second end of the hollow rod arranged to be connected by disposable connecting parts to the second container.
  • the method further comprises the step of mixing the slurry in the second container by using a connected liquid supply system for fluidizing the slurry inside the second container.
  • the second container is a chromatography column connected to a liquid supply system.
  • a homogenized slurry can be achieved within the second container and a chromatography bed can be packed within the second container.
  • an intermediate slurry tank can be avoided and the handling of slurry is simplified.
  • risk of contamination is decreased.
  • the step of providing a degree of vacuum to the second container by a vacuum production device connected to the second container comprises controlling an adaptor provided in the second container.
  • the second container comprises a liquid distribution system at an inlet for the liquid supply system hereby improving a homogenization of the slurry provided by the fluidization.
  • the second container comprises an adaptor defining the internal volume of the second container together with a bottom and internal walls of the second container, which adaptor can be used as the vacuum production device by raising it within the second container thus increasing the internal volume of the second container.
  • Figure 1 is a schematic drawing of a system according to one embodiment of the invention.
  • Figure 2a is a schematic drawing of a system according to one embodiment of the invention in an initial phase of the process.
  • Figure 2b is a schematic drawing of the same system as shown in Figure 2a but in a later phase of the process.
  • Figure 3 is a schematic drawing of a system according to one embodiment of the invention.
  • Figure 4 is a schematic drawing of a system according to one embodiment of the invention.
  • Figure 5 is a schematic drawing of a system according to one embodiment of the invention.
  • Figure 6 is a schematic drawing of a system according to one embodiment of the invention.
  • Figure 7 is a flow chart of a method according to one embodiment of the invention.
  • FIG. 1 is a schematic drawing of a system 1 according to one embodiment of the invention.
  • the system is a system for transferring a slurry of a separation resin, hereafter called a resin slurry or only a slurry, between at least two containers.
  • the separation resin can be a chromatography resin, often also called chromatography media.
  • a first container 3 and a second container 5 are shown in this embodiment.
  • a transferring connection 7 is provided between the first and the second containers 3, 5.
  • the first container 3 is a storage container for a separation resin, such as a slurry of chromatography resin.
  • the first container 3 is a flexible disposable bag.
  • the first container 3 can in this embodiment be made of for example a flexible plastic material.
  • the system 1 further comprises at least one vacuum production device 9 connected to the second container 5.
  • Said vacuum production device 9 is arranged for providing a degree of vacuum in the second container 5 at least during a transferring of the slurry resin from the first container 3 to the second container 5.
  • the vacuum production device 9 can be for example a vacuum pump or an ejector.
  • the transferring connection 7 between the first container 3 and the second container 5 comprises according to one embodiment of the invention disposable connecting parts, such as pre-sterilized tubing and aseptic connectors. In another embodiment of the invention the transferring connection 7 comprises other tubing and connectors, not necessarily pre-sterilized.
  • the slurry resin initially provided in the first container 3 can be transferred from the first container 3 to the second container 5 through the transferring connection 7 by the vacuum provided to the second container 5 by the vacuum production device 9.
  • the second container 5 is in one embodiment of the invention a slurry tank.
  • a slurry tank is used as an intermediate step between a slurry storage container and a chromatography column.
  • the slurry can often be stirred into a homogenous slurry and it can be diluted to a wanted concentration.
  • the second container 5 is the chromatography column itself. This will be further described in relation to Figure 6 below.
  • the chromatography column comprises a vacuum production device and no intermediate stirring and dilution is needed slurry could be transferred directly from a first container 3 into a chromatography column. Homogenization of the slurry could be performed inside the chromatography column itself which will be further described below.
  • the transferring connection 7 between the first container 3 and the second container 5 comprises at least one valve 11. Opening of the valve 11 will allow vacuum provided in the second container 5 to affect also the content in the first container 3 such that the slurry which initially is provided in the first container 3 is transferred to the second container 5 through the transferring connection 7.
  • a closed transferring of the slurry from the first container to the second container can be performed.
  • the slurry will only pass through the transferring connection 7.
  • Figure 2a is a schematic drawing of a system 21 according to one embodiment of the invention in an initial phase of the process and Figure 2b is a schematic drawing of the same system 21 as shown in Figure 2a but in a later phase of the process.
  • the first and second containers 3, 5, the transferring connection 7 between them and the vacuum production device 9 are given the same reference numbers as in the embodiment shown in Figure 1 and the function of these parts are the same as described in relation to Figure 1.
  • the transferring connection 7 is in this embodiment shown to comprise a first valve 23 a and a second valve 23b.
  • the second valve 23 b is provided close to the outlet of the first container 3 and can be suitable for inhibiting a resin plug which could hinder suction through the transferring connection to the second container 5.
  • the second container 5 is connected to a chromatography column 25.
  • the second container 5 is hereby not a chromatography column but rather a slurry tank.
  • the first container 3 is provided inside a rigid bin 27. If the first container 3 is a flexible bag such a rigid bin 27 is suitable for holding the first container 3 during transferring of the slurry to the second container 5.
  • the system 21 further comprises a rinse bag 29 which is connected to the first container 3.
  • Said rinse bag 29 comprises a rinsing solution, for example a buffer for rinsing the first container 3 when the slurry has been transferred to the second container 5.
  • Said rinsing solution is transferred from the rinse bag 29 to the first container 3 by the same vacuum as provided to the second container 5 for transferring the slurry.
  • a rinse valve 31 is provided in the connection between the rinse bag 29 and the at least one first container 3 such that rinsing solution is transferred form the rinse bag 29 to the first container 3 by the vacuum provided to the second container 5 by the vacuum production device 9 only when the valve 31 is open.
  • the valves 23a, 23b in the transferring connection 7 between the first container 3 and the second container 5 need to be open during the rinsing. Normally only a small amount of rinsing solution is needed for rinsing the first container 3.
  • FIG. 2B the system 21 of Figure 2A is shown in a later stage of the process when the first container 3 has been emptied. Because the first container 3 in this embodiment is a flexible bag the bag will be emptied and compacted by the vacuum, shown in Figure 2B.
  • FIG 3 is a schematic drawing of a system 41 according to one embodiment of the invention.
  • the rinse bag 29, the rinse valve 31 and the second container 5 corresponds to the same components as described in relation to the embodiment of Figure 2A and B (however in this embodiment the second container 5 can be a slurry tank or a chromatography column as in Figure 1) and keep the same reference numbers.
  • the second container 5 can be a slurry tank or a chromatography column as in Figure 1
  • a transferring connection 7 is provided between the second container 5 and the set of first containers 3 a, 3b. If the first containers as in this example are connected in series the connection 7 is provided from the last one of the first containers 3b in the series.
  • a reason for connecting more than one first containers 3 at the same time for transferring of slurry to the second container 5 is that often these storage containers for slurry (first container) are small compared to a slurry tank or chromatography column (second container). By connecting the first containers to each other (in series or in parallel) all of them can be emptied into the second container at the same time using one and the same transferring connection 7. If instead the transferring connection 7 needs to be set up for each first container 3 to be emptied into the second container 5 there may be an increased risk for contamination.
  • FIG. 4 is a schematic drawing of a system 61 according to one embodiment of the invention.
  • a transferring connection 67 between a first container 63 and a second container 5 comprises a disposable hollow rod 73 with a first end 75a of the hollow rod 73 arranged for pointing down into the first container 63 and a second end 75b of the hollow rod 73 arranged to be connected by tubing 77 and connectors, possibly disposable, pre-sterilized tubing and aseptic connectors, to the second container 5.
  • the first container 63 need not be a flexible bag but can as well be a rigid storage container for slurry.
  • FIG. 5 is a schematic drawing of a system 101 according to one embodiment of the invention. This is the same system as described in Figure 1 but including more details of the second container 5 ' .
  • the second container 5 ' is a chromatography column.
  • a first container 3' is connected to a slurry inlet 103 of the second container 5'.
  • the slurry inlet 103 is here shown provided in a bottom part of the second container 5'.
  • the second container 5' comprises also an adaptor 105 commonly used for packing a chromatography bed by compressing the slurry.
  • the adaptor 105 can be controlled to move up and down inside the chromatography column (second container) 5' and defines together with the inner walls of the second container 5' the internal volume of the chromatography column 5'.
  • a vacuum production device 9' is provided to the second container 5 ' . This could either be the control of the adaptor position. When lifting the adaptor in the closed internal volume of the second container 5' a vacuum will be provided inside the second container 5'.
  • the vacuum production device 9' could be a vacuum pump or ejector as described above.
  • the second container 5' comprises in this embodiment two liquid distribution plates and filters 107.
  • the liquid distribution plates are provided for distributing sample and buffer over the whole cross section area of the second container and the filters are provided for keeping the chromatography media inside the column.
  • One liquid distribution plate and filter is provided at a bottom of the column and one is provided to the adaptor 105.
  • the inlet 103 for the slurry allows the slurry to pass the liquid distribution plate and the filter 107.
  • a separate sample and buffer inlet 109 is also provided to a bottom part of the second container 5'.
  • the sample and buffer inlet 109 is connected to a liquid supply system 111.
  • the liquid supply system 111 is further connected to an outlet 113 from the second container 5' provided through the adaptor 105.
  • the liquid supply system comprises inlets 115 and outlets 117 for further connection to different samples and buffers to be provided to the second container 5 ' and to different collection devices for collecting sample outlet form the chromatography system.
  • Slurry can be provided from the first container 3' to the second container 5' by use of vacuum in the same way as described above in relation to the previously described embodiments, for example by opening the valve 11 ' and lifting the adaptor 105 by the vacuum production device 9'. If further homogenization of the slurry is needed before a packing procedure starts buffer, solution or water can be fluidized through the second container 5 'and thus through the slurry by the use of the liquid supply system 111 and sample and buffer inlet 109 and outlet 113. This will cause the slurry to be mixed and homogenized.
  • first containers 3' which possibly are pre filled, flexible and pre- sterilized, can be connected by pre-sterilized, disposable tubing to the chromatography column (second container 5').
  • the slurry can be provided to the chromatography column without any risk for contamination and if needed further slurry homogenization can be provided inside the column itself. Because of the homogenization step inside the second container 5 ' it is not crucial to fill the slurry in one continuous process.
  • a rinse bag 29 as described in relation to the previous embodiments can be provided also to this embodiment. This is also shown in the embodiment described below in relation to Figure 6.
  • Figure 6 is a schematic drawing of a system 201 according to one embodiment of the invention.
  • the second container 5' is also in this embodiment a chromatography column as described above in relation to Figure 5.
  • two first containers 3a', 3b' are provided connected in series in the same way as described in relation to Figure 3. They could also be connected in parallel or be more than two.
  • the transferring connection 7' is in this embodiment shown to comprise a first valve 23a' and a second valve 23b' just as described in relation to Figures 2 and 3.
  • the first containers 3a', 3b' are provided inside rigid bins 27'. If the first containers 3a', 3b'are flexible bags such a rigid bin 27' is suitable for holding the first containers 3a', 3b' during transferring of the slurry to the second container 5'.
  • the system 201 further comprises a rinse bag 29' which is connected to the first containers 3a', 3b'.
  • Said rinse bag 29' comprises a rinsing solution, for example a buffer for rinsing the first containers 3a', 3b' when the slurry has been transferred to the second container 5'.
  • Said rinsing solution is transferred from the rinse bag 29' to the first containers 3a', 3b' by the same vacuum as provided to the second container 5 ' for transferring the slurry.
  • a rinse valve 31 ' is provided in the connection between the rinse bag 29' and the at least one first containers 3a', 3b' such that rinsing solution is transferred form the rinse bag 29' to the first containers 3a', 3b' by the vacuum provided to the second container 5' by the vacuum production device 9' only when the valve 31 ' is open.
  • the valves 23a', 23b' in the transferring connection 7' between the first containers 3a', 3b' and the second container 5' need to be open during the rinsing. Normally only a small amount of rinsing solution is needed for rinsing the first containers 3a', 3b'.
  • an air sensor can be provided to the inlet to the second container.
  • a liquid supply system 111 is connected to the second container 5 ' in the same way as described above in relation to Figure 5.
  • the slurry can be homogenized by flowing a buffer, solution or water through the slurry inside the second container 5 ' before the slurry later is packed.
  • the second container 5' can comprise adaptor 105 and filter and distribution system 107 as described above in relation to Figure 5.
  • Figure 7 is a flow chart of a method for transferring a slurry of a separation resin from at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 to a second container 5, 5' according to one embodiment of the invention.
  • the method steps are described in order below: SI : Connecting the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 to the second container 5; 5' by a transferring connection 7; 7'.
  • the transferring connection 7; 7' comprises in one embodiment disposable connecting parts, which could be pre-sterilized tubing and aseptic connectors. In another embodiment the transferring connection 7; 7' comprises other, non- sterile tubing and connectors.
  • the step of allowing the vacuum provided in the second container 5; 5 ' to affect the content in the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 comprises opening a valve 11 ; 11 ' provided in the transferring connection 7; 7' between the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 and the second container 5; 5'.
  • S7 Rinsing the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 in order to assure that substantially all slurry will be transferred to the second container 5; 5'.
  • the rinsing being accomplished by transferring a rinsing solution, which could be for example a buffer, through the at least one first container 3; 3 '; 3a, 3b; 3a', 3b'; 63 and further to the second container 5; 5'.
  • the step of rinsing the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 comprises opening a rinsing valve 31; 3 ⁇ provided in the connection between the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 and the rinse bag 29; 29' to allow the vacuum provided to the second container 5; 5' to transfer rinsing solution from the rinse bag 29; 29' through the at least one first container 3; 3'; 3a, 3b; 3a', 3b'; 63 and finally to the second container 5; 5' and closing the rinse valve when a suitable amount of rinsing solution has been transferred.
  • the method comprises connecting two or more first containers 3a, 3b; 3a', 3b' in series or in parallel to the second container 5; 5' allowing the content of all of the first containers 3a, 3b; 3a', 3b' to be transferred to the second container 5; 5'.
  • S9 Mixing the slurry in the second container 5; 5'.
  • the slurry will be homogenized and can be packed into a chromatography bed. Herby the use of an intermediate slurry tank can be avoided.
  • the mixing of the slurry in the second container 5; 5' can be performed by fluidizing the slurry, for example from below as shown in Figures 5 and 6.
  • a connected liquid supply system could provide buffer or water to the second container 5, 5' and hereby the slurry can be mixed and homogenized.

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé et un système pour transférer une bouillie d'une résine de séparation, d'au moins un premier récipient à un second récipient, ledit procédé comprenant les étapes consistant à :-relier l'au moins premier récipient au second récipient par une connexion de transfert;-fournir un degré de vide au second récipient par un dispositif de production de vide relié au second récipient;-laisser le vide dans le second récipient pour affecter le contenu dans l'au moins premier récipient par le biais de la connexion de transfert de telle sorte que la suspension initialement fournie dans l'au moins premier récipient est transférée au second récipient par l'intermédiaire de la connexion de transfert.
PCT/EP2017/074902 2016-09-30 2017-09-29 Procédé et système permettant de transférer une résine de separation WO2018060496A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US16/338,117 US10940402B2 (en) 2016-09-30 2017-09-29 Method and system for transferring separation resin
CN201780073867.1A CN109982764B (zh) 2016-09-30 2017-09-29 用于传输分离树脂的方法和系统
JP2019517004A JP6983454B2 (ja) 2016-09-30 2017-09-29 分離樹脂の移送のための方法およびシステム
DK17780695.7T DK3519072T3 (da) 2016-09-30 2017-09-29 Fremgangsmåde og system til overførsel af separationsresin
EP17780695.7A EP3519072B1 (fr) 2016-09-30 2017-09-29 Procédé et système permettant de transférer une résine de separation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB1616702.5 2016-09-30
GBGB1616702.5A GB201616702D0 (en) 2016-09-30 2016-09-30 Method and system for transferring separation resin
GBGB1701576.9A GB201701576D0 (en) 2017-01-31 2017-01-31 Method and system for transferring separation resin
GB1701576.9 2017-01-31

Publications (1)

Publication Number Publication Date
WO2018060496A1 true WO2018060496A1 (fr) 2018-04-05

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US (1) US10940402B2 (fr)
EP (1) EP3519072B1 (fr)
JP (1) JP6983454B2 (fr)
CN (1) CN109982764B (fr)
DK (1) DK3519072T3 (fr)
WO (1) WO2018060496A1 (fr)

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GB201701576D0 (en) * 2017-01-31 2017-03-15 Ge Healthcare Bio Sciences Ab Method and system for transferring separation resin

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CN109982764A (zh) 2019-07-05
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