WO2018041684A1 - Verwendung von kollagenhydrolysat zur verbesserung der ausdauerleistungsfähigkeit und zur stimulation des fettabbaus - Google Patents
Verwendung von kollagenhydrolysat zur verbesserung der ausdauerleistungsfähigkeit und zur stimulation des fettabbaus Download PDFInfo
- Publication number
- WO2018041684A1 WO2018041684A1 PCT/EP2017/071184 EP2017071184W WO2018041684A1 WO 2018041684 A1 WO2018041684 A1 WO 2018041684A1 EP 2017071184 W EP2017071184 W EP 2017071184W WO 2018041684 A1 WO2018041684 A1 WO 2018041684A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- collagen hydrolyzate
- use according
- collagen
- hydrolyzate
- per day
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
- A61K38/014—Hydrolysed proteins; Derivatives thereof from animals from connective tissue peptides, e.g. gelatin, collagen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/30—Dietetic or nutritional methods, e.g. for losing weight
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/075—Ethers or acetals
- A61K31/085—Ethers or acetals having an ether linkage to aromatic ring nuclear carbon
- A61K31/09—Ethers or acetals having an ether linkage to aromatic ring nuclear carbon having two or more such linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/47—Quinolines; Isoquinolines
- A61K31/4738—Quinolines; Isoquinolines ortho- or peri-condensed with heterocyclic ring systems
- A61K31/4745—Quinolines; Isoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenantrolines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/06—Tripeptides
- A61K38/063—Glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/44—Oxidoreductases (1)
- A61K38/446—Superoxide dismutase (1.15)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
- A61K8/355—Quinones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/02—Non-specific cardiovascular stimulants, e.g. drugs for syncope, antihypotensives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/06—Preparations for care of the skin for countering cellulitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/92—Oral administration
Definitions
- the present invention relates to the use of collagen hydrolyzate for improving endurance performance.
- the invention further relates to the use of collagen hydrolyzate for the stimulation of fat loss, and in particular for the reduction of body weight.
- Collagen hydrolyzate which is produced in particular by the enzymatic hydrolysis of collagen-containing animal starting materials, consists of a mixture of peptides whose molecular weights are distributed over a certain size range depending on the starting material and production conditions.
- the use of collagen hydrolyzate as a dietary supplement has long been known, in particular for the prevention and / or treatment of disorders associated with the bone, joints or connective tissue, especially a stimulating effect of the collagen peptides on the synthesis of the body's extracellular matrix could be shown in these types of tissues (see, e.g., Bello et al., Curr. Med. Res. Opin. 2006 (22) 2221-2232).
- collagen hydrolyzate also leads to an increase in mitochondrial activity in human and animal cells, i. E. an increase in the mitochondrial count per cell and / or an enlargement of the individual mitochondria.
- collagen hydrolyzate according to the present invention can be specifically used to improve endurance performance and to stimulate fat loss in humans and animals by increasing mitochondrial activity in their muscle cells.
- the endurance capacity of the human or animal body correlates with the capacity of the aerobic metabolism to supply the muscles with the required energy in the form of ATP (adenosine triphosphate) over a longer period of time.
- Decisive for the aerobic capacity is the oxygen uptake, which in turn is determined by three factors: the oxygen supply via the lungs, the oxygen transport via the cardiovascular system and the oxygen utilization in the muscle cells. While maximum oxygen delivery is dictated by individual anatomical conditions (total alveolar surface area), oxygen transport and utilization can be increased through training and other measures, with the final step typically being the limiting factor. Endurance capacity therefore depends essentially on the number of mitochondria (per muscle cell or in the total muscle) in which the oxygen-consuming and ATP-generating reactions of the respiratory chain take place.
- an increase in mitochondrial activity means that the body's basal metabolic rate increases and a higher amount of nutrients are metabolized per unit of time for energy.
- a higher catabolic metabolic activity inevitably leads (with constant nutrient intake) to an increased degradation of the body's reserves, i. to a stimulation of fat loss.
- the degradation of the long-chain carboxylic acids released from the adipose tissue takes place essentially throughout the body and especially in the liver, so that for this aspect of the invention, as opposed to improving the endurance performance, not only the mitochondria in the muscle cells are relevant.
- the use of collagen hydrolyzate according to the invention comprises in particular a non-therapeutic use, i. administration of the collagen hydrolyzate to persons or animals that are not in need of medical treatment in terms of their endurance performance or body weight. Rather, the use is on the one hand with the aim of a general desirable increase in endurance performance. This can contribute to an improvement in the quality of life and is particularly relevant for athletes. On the other hand, a reduction in body weight by stimulating fat loss may be desirable, especially from a cosmetic point of view, ie to improve body proportions.
- the invention also encompasses the therapeutic use of collagen hydrolyzate for the prevention and / or treatment of a pathological condition characterized by a reduction in mitochondrial activity.
- a pathological condition characterized by a reduction in mitochondrial activity.
- the pathological condition may be characterized by decreased endurance performance and / or increased body weight.
- the pathological condition is preferably selected from obesity, cardiovascular diseases, cardiac arrhythmia, cardiac insufficiency, hypotension, hypertension, metabolic disorders, diabetes mellitus, metabolic syndrome, sideroblastic anemia, kidney and liver dysfunction, neuropathy, ataxia, epileptic seizures, dementia, Alzheimer's disease, autism, depression, chronic fatigue syndrome, Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, stroke-like symptoms, migraine, myoclonus, paralysis, neuralgia, hyperpathias, hyperaesthesias, dysphagia, vomiting, constipation, diarrhea, degeneration of optic nerve fibers and the retina, disturbed eye movement, ptosis, night blindness, deafness, deafness and inner ear disorders.
- a therapeutic effect can be achieved by increasing the mitochondrial or mitochondrial activity.
- the administration of collagen hydrolyzate may also have a positive effect in the prevention and / or treatment of cancer, i. of malignant tumors. This is based on the consideration that tumor cells have their energy requirements primarily through anaerobic metabolism (milk metabolism). acidic fermentation) and for this necessarily rely on glucose. An increased glucose consumption in the body cells would thus weaken the tumor cells.
- collagen hydrolyzate leads to increased expression of the enzyme AMP-activated protein kinase (AMPK).
- AMPK AMP-activated protein kinase
- This regulatory enzyme also affects the energy metabolism of the cell, so that an increase in the AMPK amount has a positive impact on endurance performance and fat loss.
- the collagen hydrolyzate is preferably administered enterally, especially orally.
- the collagen hydrolyzate is administered in the form of a dietary supplement.
- a dietary supplement particularly advantageous is the administration in the form of a solution, e.g. in the form of finished ampoules, or in the form of a powder. Due to its good solubility, the collagen hydrolyzate can also be added to various beverages without causing turbidity. By using tasteless collagen hydrolyzate, user acceptance can be increased.
- the dietary supplement according to a preferred embodiment of the invention contains no further proteins or protein hydrolysates in addition to the collagen hydrolyzate.
- Various proteins are used in known dietary supplements for muscle building and muscle maintenance, especially in athletes, with the aim of replacing carbohydrates and fats as energy suppliers to a large extent by proteins.
- the use according to the invention is not based on the function of collagen hydrolyzate as an energy source, but on the specific effect on mitochondrial activity described above.
- the dietary supplement contains no further physiologically active constituents in addition to the collagen hydrolyzate.
- the invention also includes the case that the collagen hydrolyzate is administered as part of a (food) composition with various other ingredients.
- the collagen hydrolyzate may be added to a food or beverage, e.g. A chocolate bar, protein bar or cereal bar (so-called functional food), or in milk, milk products (e.g., yoghurt) and milk substitutes (e.g., soymilk, almond milk, and coconut milk).
- the collagen hydrolyzate is typically administered in an amount of from 1 to 40 grams per day, preferably from 2.5 to 30 grams per day, more preferably from 10 to 25 grams per day, and most preferably from 12.5 to 20 grams per day .
- collagen hydrolyzate is used as the only physiologically active ingredient of a nutritional supplement, it may be combined for use in the present invention with one or more other components that have a positive effect on overall health and, more particularly, endurance performance.
- Such components are preferably selected from vitamin C, vitamins of the B, D, E and K series, conjugated linolenic acids, caffeine and its derivatives, guaranea extract, green tea extract, epigallocatechin gallate, creatine, L-carnitine, L-citrulline, L-carnitine Arginine, ⁇ -lipoic acid, N-acetylcysteine, NADH, D-ribose, magnesium aspartate, antioxidants such as anthocyanins, carotenoids, flavonoids, resveratol, glutathione, superoxide dismutase and xanthans such as mangiferin, minerals such as iron, magnesium, calcium, zinc, selenium and Phosphorus, and other proteins, hydrolys
- a further advantageous embodiment of the invention relates to the combination of the collagen hydrolyzate with ubiquinone-10 and / or ubiquinol, ie. the oxidized or reduced form of the coenzyme Qi 0 , wherein ubiquinol is preferred because of its better bioavailability.
- ubiquinol is preferred because of its better bioavailability.
- a daily intake of 50 to 100 mg of ubiquinol a positive effect on physical performance was observed, whereby promotion of mitochondrial activity is assumed by the antioxidant action of ubiquinol.
- the effect of the collagen hydrolyzate in the above indications associated with mitochondrial dysfunction can be promoted in this way.
- a combination of the collagen hydrolyzate with pyrroloquinoline quinone (PQQ) is possible, which has recently been discovered as an important redox cofactor.
- the administration of collagen hydrolyzate is combined with endurance training or altitude training.
- Endurance training can increase the aerobic capacity of the metabolism.
- a physical training under relative lack of oxygen hyperoxia training
- hypoxia training has a strong effect on the endurance performance, so that when co-administration of collagen hydrolyzate in each case a synergistic effect is assumed. This is of particular interest to athletes.
- collagen hydrolyzate takes place in the absence of endurance training, altitude training or muscle training.
- the molecular weight of the collagen hydrolyzate used may vary over a wide range according to the invention, with an upper limit imposed by the fact that collagen hydrolyzate, unlike denatured collagen or gelatin, has a sufficiently high degree of hydrolysis to be water-soluble at room temperature and not gelled.
- the soluble peptides of the collagen hydrolyzate can be well absorbed in the body.
- the collagen hydrolyzate has an average molecular weight of from 200 to 25,000 Da, preferably from 1,000 to 6,000 Da, more preferably from 1,200 to 4,000 Da, even more preferably from 1,500 to 3,500 Da, and most preferably from 2,800 to 3,300 Da.
- the collagen hydrolyzate is conveniently prepared by enzymatic hydrolysis of a collagen-containing starting material.
- endopeptidases and / or exopeptidases of microbial or plant origin are used for this hydrolysis.
- the collagen-containing starting material is usually selected from the skin or bones of vertebrates, preferably from mammals, and in particular from the skin of cattle or pigs (cattle slit or pork rind).
- the collagen hydrolyzate can be prepared from these starting materials either in a one-step process or gelatin via the intermediate, in which case both Type A and Type B gelatin can be used.
- the collagen hydrolyzate for use in the invention may be produced by recombinant gene expression.
- natural collagen sequences in particular from cattle or pigs, and their expression in genetically modified cells (eg yeasts, bacteria or plant cells, especially tobacco)
- products can be produced which are substantially identical to the hydrolysis products of the corresponding collagen-containing raw materials are . It is possible to obtain a narrower or exactly predetermined molecular weight distribution.
- the sequences can be altered by mutations to affect certain properties of the product.
- An object of the present invention is further a method of improving endurance performance and / or stimulating fat loss, and more particularly, reducing body weight by increasing mitochondrial activity.
- the method preferably comprises the enteral, in particular oral, administration of collagen hydrolyzate to a human or to an animal.
- the method can be both a therapeutic and a non-therapeutic method.
- Figure 1 fluorescence micrographs of SH-SY5Y cells, which were incubated in the presence of collagen hydrolyzate.
- the SH-SY5Y cells were incubated in culture media with different concentrations of collagen hydrolyzate of 0.05% by weight, 0.2% by weight and 2.5% by weight.
- collagen hydrolyzate A a collagen hydrolyzate of pork rind gelatin having an average molecular weight in the range of 3,000 Da prepared by enzymatic hydrolysis (hereinafter referred to as collagen hydrolyzate A) was used.
- the molecular weight distribution of the peptides which was determined by gel permeation chromatography, is given in the following Table 1: Table 1: MG distribution collagen hydrolyzate A
- TOM20 mitochondrial protein component TOM20 was fluorescently labeled.
- TOM20 is a subunit of a receptor complex in the outer membrane of the mitochondria, which has the function to transfer cytosolic precursor proteins (prepeptides) into the mitochondria. There, the proteins, which are enzymes of the respiratory chain or the citric acid cycle, are activated by cleavage of the presequence.
- the amount of fluorescently labeled TOM20 visible in the fluorescence microscope is thus a measure of the mitochondrial number in the cell.
- the cells incubated with 0.05% by weight, 0.2% by weight and 2.5% by weight of collagen hydrolyzate are shown in FIGS. 1A, 1B and 1C respectively, with an increase of the light (in the original green) with increasing concentration. Fluorescence in the areas around the nucleus (blue in the original) is clearly visible.
- the collagen hydrolyzate thus causes an increase in the mitochondrial count in the SH-SY5Y cells, and thus an increase in the total mitochondrial activity.
- AMP-activated protein kinase is involved in energy delivery in both adipose tissue and muscle. Since AMP is formed when consuming ATP, it can be considered as an indicator of energy shortage become. The expression of AMPK thus serves to activate energy reserves from the fat depot and in the context of glycolysis.
- AMPK RNA was significantly increased (by a factor of over 600). This finding also results in a stimulating influence of collagen hydrolyzate on the energy metabolism of the cell.
- mice were fed daily with a quantity of collagen hydrolyzate corresponding to a human equivalence dose of 10 g over a period of 3 months. After the mice had been euthanized, the quadriceps were completely excised, snap frozen and ground. From the muscle tissue, the soluble proteins were extracted and the amount of AMPK determined by means of an immunoassay (ELISA).
- ELISA immunoassay
- the amount of AMPK was increased by a factor between 1.5 and 2.
- the test group and the control group each comprised six animals.
- a fine needle biopsy was taken from the four-headed thigh muscle (quadriceps femoris) in each rat.
- the animals of the test groups were then given a daily dose of 200 mg of the respective collagen hydrolyzate (see below) per kg of the current body weight (corresponding to a daily dose of 15 g in a 75 kg person) over a period of four weeks.
- the collagen hydrolyzate was added at a concentration of 20 mg / ml in an appropriate amount dissolved in tap water and administered via a nasogastric tube.
- the animals of the control group each received the identical amount of tap water without collagen hydrolyzate.
- a collagen hydrolyzate B from bovine cleavage gelatin having an average molecular weight of 2,000 Da was used in further test groups, and a collagen hydrolyzate C from bovine cleavage gelatin having an average molecular weight of 3,500 Da, each produced by enzymatic hydrolysis.
- the molecular weight distributions of all three hydrolysates are given in the following Table 2:
- the value "Cohen's d" as a measure of the effect size is more than 2.5 for all test groups, which is a very strong effect.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Nutrition Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Gastroenterology & Hepatology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Cell Biology (AREA)
- Emergency Medicine (AREA)
Abstract
Description
Claims
Priority Applications (15)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG11201901043RA SG11201901043RA (en) | 2016-08-30 | 2017-08-23 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism |
BR112019002725-4A BR112019002725A2 (pt) | 2016-08-30 | 2017-08-23 | uso de hidrolisado de colágeno para melhorar a capacidade de resistência e para estimular a degradação de gordura |
KR1020197004688A KR20190042006A (ko) | 2016-08-30 | 2017-08-23 | 지구력 성능을 향상시키고 지질 이화작용을 자극하기 위한 콜라겐 가수분해물의 용도 |
AU2017320520A AU2017320520A1 (en) | 2016-08-30 | 2017-08-23 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipocatabolism |
CA3035281A CA3035281A1 (en) | 2016-08-30 | 2017-08-23 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism |
EP17758489.3A EP3506929A1 (de) | 2016-08-30 | 2017-08-23 | Verwendung von kollagenhydrolysat zur verbesserung der ausdauerleistungsfähigkeit und zur stimulation des fettabbaus |
IL264812A IL264812B2 (en) | 2016-08-30 | 2017-08-23 | Using collagen hydrolyzate to improve endurance performance and stimulate fat catabolism |
EA201990579A EA201990579A1 (ru) | 2016-08-30 | 2017-08-23 | Применение гидролизата коллагена для улучшения способности к выносливости и стимуляции расщепления жиров |
JP2019511356A JP2019529365A (ja) | 2016-08-30 | 2017-08-23 | 持久運動能力を向上させるためおよび脂質代謝を刺激するためのコラーゲン加水分解物の使用 |
MX2019002335A MX2019002335A (es) | 2016-08-30 | 2017-08-23 | Uso de hidrolizado de colageno para mejorar el rendimiento de resistencia y para estimular el catabolismo lipidico. |
CN201780053530.4A CN109715197A (zh) | 2016-08-30 | 2017-08-23 | 胶原水解产物用于改善耐力表现和刺激脂质分解代谢的用途 |
TNP/2019/000037A TN2019000037A1 (en) | 2016-08-30 | 2017-08-23 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipocatabolism |
US16/285,874 US11235034B2 (en) | 2016-08-30 | 2019-02-26 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism |
ZA2019/01542A ZA201901542B (en) | 2016-08-30 | 2019-03-12 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipocatabolism |
US17/552,499 US12023369B2 (en) | 2016-08-30 | 2021-12-16 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102016116160 | 2016-08-30 | ||
DE102016116160.8 | 2016-08-30 | ||
DE102017102873.0 | 2017-02-14 | ||
DE102017102873.0A DE102017102873A1 (de) | 2016-08-30 | 2017-02-14 | Verwendung von Kollagenhydrolysat zur Verbesserung der Ausdauerleistungsfähigkeit und zur Stimulation des Fettabbaus |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/285,874 Continuation US11235034B2 (en) | 2016-08-30 | 2019-02-26 | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2018041684A1 true WO2018041684A1 (de) | 2018-03-08 |
Family
ID=68039643
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2017/071184 WO2018041684A1 (de) | 2016-08-30 | 2017-08-23 | Verwendung von kollagenhydrolysat zur verbesserung der ausdauerleistungsfähigkeit und zur stimulation des fettabbaus |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP3506929A1 (de) |
KR (1) | KR20190042006A (de) |
CN (1) | CN109715197A (de) |
AU (1) | AU2017320520A1 (de) |
BR (1) | BR112019002725A2 (de) |
CA (1) | CA3035281A1 (de) |
CL (1) | CL2019000529A1 (de) |
MX (1) | MX2019002335A (de) |
SG (1) | SG11201901043RA (de) |
WO (1) | WO2018041684A1 (de) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108685971A (zh) * | 2018-06-12 | 2018-10-23 | 泓博元生命科技(深圳)有限公司 | 一种减肥组合物及其制备方法和应用 |
WO2019166418A1 (de) * | 2018-02-28 | 2019-09-06 | Gelita Ag | Nutrazeutische oder pharmazeutische zusammensetzung |
WO2020038685A1 (de) * | 2018-08-22 | 2020-02-27 | Gelita Ag | Proteinriegel |
WO2020038768A1 (de) * | 2018-08-20 | 2020-02-27 | Gelita Ag | Getränk und feststoffmischung zu seiner herstellung |
WO2020127929A1 (de) * | 2018-12-21 | 2020-06-25 | Gelita Ag | Synthetische und rekombinant hergestellte kollagenpeptide mit biologischer wirksamkeit |
JP2021038147A (ja) * | 2019-08-30 | 2021-03-11 | 国立大学法人 鹿児島大学 | ミトコンドリア生合成促進剤 |
US11028148B2 (en) | 2017-09-28 | 2021-06-08 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
US11168126B2 (en) | 2019-04-12 | 2021-11-09 | Geltor, Inc. | Recombinant elastin and production thereof |
US11174300B2 (en) | 2020-01-24 | 2021-11-16 | Geltor, Inc. | Animal-free dietary collagen |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11998654B2 (en) | 2018-07-12 | 2024-06-04 | Bard Shannon Limited | Securing implants and medical devices |
TW202027734A (zh) * | 2019-04-01 | 2020-08-01 | 許悅郎 | 預防及/或治療失智症之組合物 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102210855A (zh) * | 2011-04-20 | 2011-10-12 | 中国海洋大学 | 一种海洋特征寡糖与胶原蛋白肽的复配物及其制备方法和应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002255846A (ja) * | 2001-02-26 | 2002-09-11 | Sunstar Inc | 経口組成物 |
-
2017
- 2017-08-23 EP EP17758489.3A patent/EP3506929A1/de active Pending
- 2017-08-23 SG SG11201901043RA patent/SG11201901043RA/en unknown
- 2017-08-23 KR KR1020197004688A patent/KR20190042006A/ko not_active IP Right Cessation
- 2017-08-23 WO PCT/EP2017/071184 patent/WO2018041684A1/de unknown
- 2017-08-23 AU AU2017320520A patent/AU2017320520A1/en active Pending
- 2017-08-23 MX MX2019002335A patent/MX2019002335A/es unknown
- 2017-08-23 CA CA3035281A patent/CA3035281A1/en active Pending
- 2017-08-23 CN CN201780053530.4A patent/CN109715197A/zh active Pending
- 2017-08-23 BR BR112019002725-4A patent/BR112019002725A2/pt unknown
-
2019
- 2019-02-27 CL CL2019000529A patent/CL2019000529A1/es unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102210855A (zh) * | 2011-04-20 | 2011-10-12 | 中国海洋大学 | 一种海洋特征寡糖与胶原蛋白肽的复配物及其制备方法和应用 |
Non-Patent Citations (12)
Title |
---|
A. GLAUERT UND P: "Biological Specimen Preparation for Transmission Electron Microscopy", PRINCETON LEGACY LIBRARY, 2014 |
ANONYMOUS: "5 Benefits of Collagen Protein | Myprotein | The Zone", 29 July 2015 (2015-07-29), XP055414659, Retrieved from the Internet <URL:https://web.archive.org/web/20150729133612/http://www.myprotein.com:80/thezone/nutrition/5-amazing-benefits-collagen-protein/> [retrieved on 20171011] * |
ANONYMOUS: "Hydrolysed Liquid Collagen", 9 November 2015 (2015-11-09), XP055414242, Retrieved from the Internet <URL:http://naturem.co.uk/2015/11/09/hydrolysed-liquid-collagen/> [retrieved on 20171010] * |
BELLO ET AL., CURR. MED. RES. OPIN., no. 22, 2006, pages 2221 - 2232 |
DENISE ZDZIEBLIK ET AL: "Collagen peptide supplementation in combination with resistance training improves body composition and increases muscle strength in elderly sarcopenic men: a randomised controlled trial", BRITISH JOURNAL OF NUTRITION, vol. 114, no. 8, 28 October 2015 (2015-10-28), UK, pages 1237 - 1245, XP055414225, ISSN: 0007-1145, DOI: 10.1017/S0007114515002810 * |
ELENA BARBIERI ET AL: "Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity", OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, vol. 2016, 1 January 2016 (2016-01-01), US, pages 1 - 12, XP055414221, ISSN: 1942-0900, DOI: 10.1155/2016/5152029 * |
ELISÂNGELA PORFÍRIO ET AL: "Collagen supplementation as a complementary therapy for the prevention and treatment of osteoporosis and osteoarthritis: a systematic review", REVISTA BRASILEIRA DE GERIATRIA E GERONTOLOGIA, vol. 19, no. 1, 1 February 2016 (2016-02-01), pages 153 - 164, XP055414276, ISSN: 1809-9823, DOI: 10.1590/1809-9823.2016.14145 * |
KITAKAZE TOMOYA ET AL: "The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 478, no. 3, 21 August 2016 (2016-08-21), pages 1292 - 1297, XP029726790, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2016.08.114 * |
MATHIEU FERRON ET AL: "Intermittent injections of osteocalcin improve glucose metabolism and prevent type 2 diabetes in mice", BONE, PERGAMON PRESS., OXFORD, GB, vol. 50, no. 2, 19 April 2011 (2011-04-19), pages 568 - 575, XP028441379, ISSN: 8756-3282, [retrieved on 20110429], DOI: 10.1016/J.BONE.2011.04.017 * |
OSAMU HASHIZUME ET AL: "Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects", SCIENTIFIC REPORTS, vol. 5, no. 1, 1 September 2015 (2015-09-01), XP055411089, DOI: 10.1038/srep10434 * |
STEFAN M. SCHIEKE ET AL: "The Mammalian Target of Rapamycin (mTOR) Pathway Regulates Mitochondrial Oxygen Consumption and Oxidative Capacity", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 281, no. 37, 15 September 2006 (2006-09-15), pages 27643 - 27652, XP055414347, ISSN: 0021-9258, DOI: 10.1074/jbc.M603536200 * |
T OKIURA ET AL: "Effects of collagen hydrolysate on the tibialis anterior muscle and femur in senescence-accelerated mouse prone 6", JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS, 1 January 2016 (2016-01-01), Greece, pages 161, XP055414203, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114359/pdf/JMNI-16-161.pdf> * |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11180541B2 (en) | 2017-09-28 | 2021-11-23 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
US11028148B2 (en) | 2017-09-28 | 2021-06-08 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
US11041015B2 (en) | 2017-09-28 | 2021-06-22 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
US11214609B2 (en) | 2017-09-28 | 2022-01-04 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
WO2019166418A1 (de) * | 2018-02-28 | 2019-09-06 | Gelita Ag | Nutrazeutische oder pharmazeutische zusammensetzung |
CN108685971A (zh) * | 2018-06-12 | 2018-10-23 | 泓博元生命科技(深圳)有限公司 | 一种减肥组合物及其制备方法和应用 |
WO2020038768A1 (de) * | 2018-08-20 | 2020-02-27 | Gelita Ag | Getränk und feststoffmischung zu seiner herstellung |
WO2020038685A1 (de) * | 2018-08-22 | 2020-02-27 | Gelita Ag | Proteinriegel |
JP7445646B2 (ja) | 2018-08-22 | 2024-03-07 | ゲリタ アクチェンゲゼルシャフト | プロテインバー |
JP2021533807A (ja) * | 2018-08-22 | 2021-12-09 | ゲリタ アクチェンゲゼルシャフト | プロテインバー |
WO2020127929A1 (de) * | 2018-12-21 | 2020-06-25 | Gelita Ag | Synthetische und rekombinant hergestellte kollagenpeptide mit biologischer wirksamkeit |
JP2022516423A (ja) * | 2018-12-21 | 2022-02-28 | ジェリータ アーゲー | 生物学的有効性を示す合成および組換えにより製造されたコラーゲンペプチド |
US11168126B2 (en) | 2019-04-12 | 2021-11-09 | Geltor, Inc. | Recombinant elastin and production thereof |
JP2021038147A (ja) * | 2019-08-30 | 2021-03-11 | 国立大学法人 鹿児島大学 | ミトコンドリア生合成促進剤 |
US11174300B2 (en) | 2020-01-24 | 2021-11-16 | Geltor, Inc. | Animal-free dietary collagen |
US11332505B2 (en) | 2020-01-24 | 2022-05-17 | Geltor, Inc. | Animal-free dietary collagen |
Also Published As
Publication number | Publication date |
---|---|
CN109715197A (zh) | 2019-05-03 |
KR20190042006A (ko) | 2019-04-23 |
CL2019000529A1 (es) | 2019-07-26 |
MX2019002335A (es) | 2019-05-16 |
CA3035281A1 (en) | 2018-03-08 |
BR112019002725A2 (pt) | 2019-05-14 |
EP3506929A1 (de) | 2019-07-10 |
SG11201901043RA (en) | 2019-03-28 |
AU2017320520A1 (en) | 2019-03-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2018041684A1 (de) | Verwendung von kollagenhydrolysat zur verbesserung der ausdauerleistungsfähigkeit und zur stimulation des fettabbaus | |
WO2019166418A1 (de) | Nutrazeutische oder pharmazeutische zusammensetzung | |
DE102008036954B4 (de) | Verwendung einer Aminozucker enthaltenden Zusammensetzung | |
US20060269617A1 (en) | Supplement compositions and method of use for enhancement of insulin sensitivity | |
DE19528461A1 (de) | Präparat zur Ernährung | |
US20210369804A1 (en) | Withania somnifera composition, method of preparation and use thereof | |
EP3064209B1 (de) | Zubereitung enthaltend ginsenosid f2 zur prävention oder behandlung der insulinresistenz | |
DE102017102873A1 (de) | Verwendung von Kollagenhydrolysat zur Verbesserung der Ausdauerleistungsfähigkeit und zur Stimulation des Fettabbaus | |
US20130115195A1 (en) | Anti-Aging Formulations | |
DE69633818T2 (de) | Verwendung eines mittels zur zur vorbeugung oder behandlung von durch anomalitäten des knorpelgewebes verursachten erkrankungen | |
DE102007053369A1 (de) | Verwendung einer eine Kreatin-Komponente enthaltende Zusammensetzung zur Verbesserung der männlichen Fruchtbarkeit | |
EP2094116B1 (de) | Feste oder wässrige alkalische zubereitung umfassend eine kreatin-komponente, verfahren zu deren herstellung und ihre verwendung | |
TWI830696B (zh) | 抑制肌纖維變性用組成物 | |
US20060189566A1 (en) | Muscle building agent and preventive or remedy for muscle weakening | |
JP2009013083A (ja) | 経口投与組成物 | |
EP4051706A1 (de) | Ernährungsphysiologisch optimiertes kollagenpeptid | |
AT515336A1 (de) | Vermeidung oxidativer Prozesse und oxidativem Stress | |
AT510810A1 (de) | Kombinationspräparat zur verbesserung der weiblichen fertilität | |
EP3131565A2 (de) | Präparat aus hafermehl und verfahren zu seiner verwendun | |
WO2020182831A1 (de) | Nahrungsergänzungsmittel | |
US12023369B2 (en) | Use of collagen hydrolysate for improving endurance performance and for stimulating lipid catabolism | |
CN105360798A (zh) | 一种增强耐力和爆发力的玛咖组方及其制备方法 | |
KR102564832B1 (ko) | 반려동물의 털빠짐 예방용 조성물 | |
DE102005031363A1 (de) | Mittel mit Anti-Aging-Effekt | |
DE102006002097A1 (de) | Zusammensetzung und Anwendung eines oralen Mittels zu Brunstauslösung und deren Herstellung |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 17758489 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 20197004688 Country of ref document: KR Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2019511356 Country of ref document: JP Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 3035281 Country of ref document: CA |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112019002725 Country of ref document: BR |
|
ENP | Entry into the national phase |
Ref document number: 2017320520 Country of ref document: AU Date of ref document: 20170823 Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2017758489 Country of ref document: EP Effective date: 20190401 |
|
ENP | Entry into the national phase |
Ref document number: 112019002725 Country of ref document: BR Kind code of ref document: A2 Effective date: 20190211 |