WO2017204561A3 - 리포터 모이어티, 기질 모이어티 및 탈안정화 모이어티를 포함하는 폴리펩티드를 코딩하는 재조합 폴리뉴클레오티드, 그를 포함하는 숙주세포, 및 그의 용도 - Google Patents

리포터 모이어티, 기질 모이어티 및 탈안정화 모이어티를 포함하는 폴리펩티드를 코딩하는 재조합 폴리뉴클레오티드, 그를 포함하는 숙주세포, 및 그의 용도 Download PDF

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Publication number
WO2017204561A3
WO2017204561A3 PCT/KR2017/005420 KR2017005420W WO2017204561A3 WO 2017204561 A3 WO2017204561 A3 WO 2017204561A3 KR 2017005420 W KR2017005420 W KR 2017005420W WO 2017204561 A3 WO2017204561 A3 WO 2017204561A3
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Prior art keywords
moiety
same
polypeptide
host cell
destabilizing
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PCT/KR2017/005420
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English (en)
French (fr)
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WO2017204561A2 (ko
Inventor
정현호
양기혁
이준호
이동규
이영래
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(주)메디톡스
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Priority to US16/098,278 priority Critical patent/US11198859B2/en
Priority to CN201780027864.4A priority patent/CN109153997A/zh
Priority to JP2018560622A priority patent/JP6741788B2/ja
Priority to EP17803080.5A priority patent/EP3438267B1/en
Publication of WO2017204561A2 publication Critical patent/WO2017204561A2/ko
Publication of WO2017204561A3 publication Critical patent/WO2017204561A3/ko

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    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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Abstract

리포터 모이어티, 기질 모이어티 및 탈안정화 모이어티를 포함하는 폴리펩티드를 코딩하는 재조합 폴리뉴클레오티드, 그를 포함하는 숙주세포, 및 그를 이용하여 프로테아제의 수준을 측정하는 방법을 제공한다.
PCT/KR2017/005420 2016-05-04 2017-05-24 리포터 모이어티, 기질 모이어티 및 탈안정화 모이어티를 포함하는 폴리펩티드를 코딩하는 재조합 폴리뉴클레오티드, 그를 포함하는 숙주세포, 및 그의 용도 WO2017204561A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US16/098,278 US11198859B2 (en) 2016-05-04 2017-05-24 Recombinant polynucleotide coding for polypeptide comprising reporter moiety, substrate moiety and destabilizing moiety, host cell comprising same and use of same
CN201780027864.4A CN109153997A (zh) 2016-05-24 2017-05-24 编码含报告分子、底物和去稳定化部分的多肽的重组多核苷酸，含其的宿主细胞及其用途
JP2018560622A JP6741788B2 (ja) 2016-05-24 2017-05-24 レポーターモイエティ、基質モイエティ及び脱安定化モイエティを含むポリペプチドをコーディングする組み換えポリヌクレオチド、それを含む宿主細胞、並びにその用途
EP17803080.5A EP3438267B1 (en) 2016-05-24 2017-05-24 Recombinant polynucleotide coding for polypeptide comprising reporter moiety, substrate moiety and destabilizing moiety, host cell comprising same and use of same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0063722 2016-05-24
KR20160063722 2016-05-24

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WO2017204561A2 WO2017204561A2 (ko) 2017-11-30
WO2017204561A3 true WO2017204561A3 (ko) 2018-01-18

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PCT/KR2017/005420 WO2017204561A2 (ko) 2016-05-04 2017-05-24 리포터 모이어티, 기질 모이어티 및 탈안정화 모이어티를 포함하는 폴리펩티드를 코딩하는 재조합 폴리뉴클레오티드, 그를 포함하는 숙주세포, 및 그의 용도

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US (1) US11198859B2 (ko)
EP (1) EP3438267B1 (ko)
JP (1) JP6741788B2 (ko)
KR (1) KR101981199B1 (ko)
CN (1) CN109153997A (ko)
WO (1) WO2017204561A2 (ko)

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GB201702500D0 (en) * 2017-02-16 2017-04-05 Univ Sheffield Stable vamp reporter assay
WO2024107023A1 (ko) * 2022-11-18 2024-05-23 주식회사 바이오링크스 재조합 형광단백질을 이용한 세포 기반 보툴리눔 독소의 검출 또는 활성 측정 방법

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KR101981199B1 (ko) 2019-05-22
JP6741788B2 (ja) 2020-08-19
EP3438267A4 (en) 2019-08-21
JP2019516379A (ja) 2019-06-20
EP3438267A2 (en) 2019-02-06
US11198859B2 (en) 2021-12-14
WO2017204561A2 (ko) 2017-11-30
US20190161745A1 (en) 2019-05-30
EP3438267B1 (en) 2024-05-01
CN109153997A (zh) 2019-01-04
KR20170132697A (ko) 2017-12-04

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