WO2017042568A1 - Compléments pour la santé - Google Patents

Compléments pour la santé Download PDF

Info

Publication number
WO2017042568A1
WO2017042568A1 PCT/GB2016/052779 GB2016052779W WO2017042568A1 WO 2017042568 A1 WO2017042568 A1 WO 2017042568A1 GB 2016052779 W GB2016052779 W GB 2016052779W WO 2017042568 A1 WO2017042568 A1 WO 2017042568A1
Authority
WO
WIPO (PCT)
Prior art keywords
honey
composition
bee
microbiota
bee feed
Prior art date
Application number
PCT/GB2016/052779
Other languages
English (en)
Inventor
Jonah Chitolie
Original Assignee
Corbicula Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Corbicula Limited filed Critical Corbicula Limited
Priority to GB1805634.1A priority Critical patent/GB2564514A/en
Publication of WO2017042568A1 publication Critical patent/WO2017042568A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K59/00—Honey collection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Definitions

  • the present invention relates to a health supplement or probiotic composition, to a method for its production and to its use in the treatment for introducing microbiota into the human gut for improving the health of a recipient and/or treating a disease. It also relates to the treatment of a gastrointestinal disorder by administration of a composition as aforesaid and to a purpose-related health supplement or probiotic composition. The invention also relates to a bee feed and to its use.
  • Faecal Microbiota Transplant FMT Faecal Microbiota Transplant
  • This is a clinical process wherein patients lacking essential Microflora needed for their gut to function properly, are treated by implanting the beneficial intestinal bacteria and yeasts from a healthy donor.
  • the main procedures for restoring gut microbiota are by enema, or by mouth in the form of a capsule containing freeze-dried material, obtained from a healthy donor.
  • FMT has been successfully been used for the treatment of gastrointestinal diseases which include colitis, constipation and irritable bowel syndrome and infection with Clostridium difficile.
  • An object of the invention is to provide an alternative, or an improved, way of introducing microbiota into the human gut.
  • honey can provide a useful vehicle for storage and/or administration of human gut microbiota e.g. a colonic microbiota.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic.
  • the invention provides a novel method of introducing and restoring healthy human gut microbiota by adding healthy human gut microbiota, donated by healthy individuals, into treated honey and plant sugars, thus forming an oral preparation, which may be in capsule form.
  • the invention provides a method of treating a gastrointestinal disorder which comprises administering to a patient suffering from such disorder a composition comprising human gut microbiota and honey or a honey mimic.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of a gastrointestinal disorder.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of any of colitis, constipation, irritable bowel syndrome and infection with Clostridium difficile.
  • the invention provides a bee feed bee feed based on fungi fortified with compounds from plants that are medicinal. Description of preferred embodiments
  • the human gut microbiota for incorporation into the above mentioned composition may be obtained via a fecal microbial transplant technique.
  • the microbiota may be an entire fecal microbiota, a treated or untreated fecal flora sample, a complete or partial fecal flora sample, a fecal flora substantially or completely purified of non-fecal floral fecal material or a partially, substantially or completely isolated or purified fecal flora which may be lyophilized, freeze-dried or frozen, or processed into a powder.
  • a crude collected stool may be filtered and/or homogenized, and then its bacterial cells may be separated (e.g., from unwanted fibrous material) by plasmapheresis, centrifugation, centrifuge, column chromatography (e.g., affinity chromatography) or immunoprecipitation (e.g., antibodies fixed to a solid surface, such as beads or a plate).
  • plasmapheresis e.g., centrifugation, centrifuge, column chromatography (e.g., affinity chromatography) or immunoprecipitation (e.g., antibodies fixed to a solid surface, such as beads or a plate).
  • column chromatography e.g., affinity chromatography
  • immunoprecipitation e.g., antibodies fixed to a solid surface, such as beads or a plate. Freeze dried fecal microbiota is described in US 2015/374761 (Sadowsky et al.).
  • the microbiota may comprise different classes of bacteria selected from the group consisting of Actinobacteria, Bacteroidia, Bacilli, Clostridia, Erysipelotrichi, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Mollicutes, and Verrucomicrobiae.
  • the composition may comprise the human gut microbiota and a carrier which is honey free from or having a reduced content of honey bee microbiota. Additionally or alternatively, it may comprise a honey mimic comprising at least one of glucose, maltose, glycerol, sucrose and fructose.
  • honey or honey mimic may be sterilized, and may be maintained in an anaerobic state.
  • Patent Application WO 2016/038348 is described a method and apparatus for the analysis of honey and there is mentioned that the results of such analysis, together with observations and analysis of the content of a beehive, provide information on what supplements may be beneficial both to bees and humans.
  • honey could be a reservoir for bacteria, yeasts and moulds. Both aerobic, spore producing anaerobic bacteria and yeast are found in honey. Sources of microorganisms in honey are from pollen, nectar, digestive tract of honey bees and the bee hive. Spore producing anaerobic bacteria are likely to be from the digestive tract of honey bees.
  • Microbiota in honey is inactive, this may be due to low water content and high concentrations of sugars. Accordingly, healthy human gut microbiota can be stored in honey for a long period of time.
  • a human health supplement comprises honey, or a honey mimic, containing human gut microbiota.
  • Other sugar solutions including glucose, maltose, glycerol, sucrose and fructose can mimic the same concentration and specific density of honey as a bacteriostatic storage medium.
  • honey may be supplied in a suitable labelled jar, it may also be supplied in capsules with a diverse range of shell ingredient, including Shellac, soya wax, beeswax, gelatine, cellulose. They may be offered with and without enteric coating protection to allow for timed release of the microbiota at a targeted area of the human intestine or not.
  • shell ingredient including Shellac, soya wax, beeswax, gelatine, cellulose.
  • They may be offered with and without enteric coating protection to allow for timed release of the microbiota at a targeted area of the human intestine or not.
  • Capsules containing honey suspension of human gut flora are stable at room temperature and have a very long shelf life.
  • Preparations, including capsules, with healthy microbiota according to the invention may be enhanced with specific bacteria to treat particular medical conditions.
  • Having a healthy and diverse gut microbiota can contribute to our physiology, metabolism, nutrition, endocrine, neurological and immune systems. Delivery of good bacteria or good gut microbiota can be made by mixing good bacteria and/or gut microbiota with honey or similar density sugar syrups as listed above, where the honey has had the honey bee microbiota removed or neutralised through sterilisation. Time-release capsules of honey containing healthy human microbiota will enable healthy microbiota to be released into the intestine. Taken regularly over a period of time will enable a healthy and diverse gut microbiota to develop which contributes to overall health and fitness.
  • the concentration of Microbiota used in supplement may be in the order of:
  • CFU colony-forming unit
  • microbiota is captured from healthy donor faeces and stored in honey under anaerobic conditions, the honey having been either heat treated and filtered to remove the honey bee microbiota or sterilized to neutralize it.
  • FMT Fecal Microbial Transplant
  • a quantity of honey is heated and filtered to remove the honey bee microbiota.
  • the microbiota is then stored in the honey under anaerobic conditions in a concentration as follows:
  • Capsules are then created having an enteric coating comprising a mixture of polymeric acid with shellac.
  • An alternative aspect of the invention relates to a bee food supplement.
  • Bees gather fungal spores in significant amounts and also collect pollen from plants that have medicinal properties.
  • a bee feed is provided based on fungi (such as Quorn) fortified with compounds from plants that are medicinal.
  • Medicinal feeds can be used to fight viral and bacterial infections as well as to supply the bee colony with essential nutrients for the development of healthy bees.
  • Using the same principle natural plant substances that deters parasites can alleviate bee colonies of parasitic mites such as Varroa.
  • Such medicinal feeds can be given to colonies any time when needed, throughout the foraging season. Since such feeds need have no sugars they will not affect the flavour of honey produced.
  • Chytrids are the only organisms that can produce enzymes that breaks down sporopollenin. Chytrids are susceptible to pesticides especially fungicides. Areas which fungicides are heavily used therefore may lack large numbers of Chytrids which directly affect the amount of p-coumaric acid produced from the breakdown of sporopollenin.
  • honey bees gather contains many compounds that are essential to the general health and fitness of the honey bee colony.
  • Honey bees collect pollen from many medicinal herbs and plants which may prevent specific diseases and parasites.
  • Honey bees collect fungi which are associated with pollen breakdown by breaking down the inner wall of pollen and pollen contents.
  • 2016/038348 recovers pollen at different stages of breakdown. It has been observed that a type of fungi (Chytrids) are sometimes found growing on pollen.
  • Varroa destructor One of the most devastating parasites which have become widespread in bee hives is Varroa destructor.
  • a bee supplement can be made from pollen from plants which has insecticidal properties as described above.
  • Neem plant Azadirachta indica
  • Pollen from Neem can be broken down to provide a concentration of insecticide which may not affect the honey bee but can deter the feeding of Varroa and hence reduce/remove the parasite from bee hives.
  • Example 2 honey bee supplement
  • a honey bee supplement is made by choosing plants which contain specific compounds known for medicinal benefits.
  • An example is St Johns Wort (Hypericum perforatum ⁇ which contains the compound hypericin known for antiviral properties.
  • Anthers of St Johns wort are gathered in a container and crushed with a glass rod. Water is added and washed through a 150 micron sieve mesh as described in Patent Application WO 2016/038348 to remove anthers and large plant matter.
  • the filtered liquid containing pollen is collected and washed through a 10 micron sieve mesh as described in Patent Application WO 2016/038348.
  • the solution is mixed with mycoprotein (Quorn) to produce small patties and placed in the hive, this can be carried out any time of the year and will not affect the honey quality.
  • All products of breakdown can be mixed with Quorn to produce patties, or can be mixed with a sugar feed mainly before winter.
  • Another way of introducing a liquid supplement is to mix with a sugar feed or honey. This is fed to the bees after the main honey flow is over and before winter.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Birds (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne une composition probiotique utilisée, par exemple, pour le traitement de la colite, de la constipation, du syndrome du côlon irritable ou de l'infection causée par Clostridium difficile, comprenant un microbiote intestinal et du miel ou un succédané du miel. La composition peut comprendre le microbiote intestinal humain et un support qui est du miel dépourvu ou ayant une teneur réduite en microbiote d'abeille, ou elle peut comprendre le microbiote intestinal humain et un support qui est un succédané du miel comprenant au moins du glucose, du maltose, du glycérol, du saccharose ou du fructose. Dans un aspect, l'invention concerne un aliment pour abeilles à base de champignons enrichis avec des composés issus de plantes médicinales. Ledit aliment pour abeilles peut être mélangé avec un aliment sucré ou du miel.
PCT/GB2016/052779 2014-09-08 2016-09-08 Compléments pour la santé WO2017042568A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1805634.1A GB2564514A (en) 2014-09-08 2016-09-08 Health supplements

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1415818.2A GB2529893A (en) 2014-09-08 2014-09-08 Apparatus and method for separating pollen and optionally microbiota from honey
PCT/GB2015/052583 WO2016038348A1 (fr) 2014-09-08 2015-09-08 Procédé d'analyse des éléments microscopiques contenus dans le miel
GBPCT/GB2015/052583 2015-09-08

Publications (1)

Publication Number Publication Date
WO2017042568A1 true WO2017042568A1 (fr) 2017-03-16

Family

ID=51796327

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/GB2015/052583 WO2016038348A1 (fr) 2014-09-08 2015-09-08 Procédé d'analyse des éléments microscopiques contenus dans le miel
PCT/GB2016/052779 WO2017042568A1 (fr) 2014-09-08 2016-09-08 Compléments pour la santé

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/GB2015/052583 WO2016038348A1 (fr) 2014-09-08 2015-09-08 Procédé d'analyse des éléments microscopiques contenus dans le miel

Country Status (2)

Country Link
GB (2) GB2529893A (fr)
WO (2) WO2016038348A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2688354C1 (ru) * 2018-03-12 2019-05-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Способ сохранности пчел в зимний период
US11185080B2 (en) 2014-04-30 2021-11-30 Matoke Holdings Limited Antimicrobial compositions
US11730168B2 (en) 2017-10-16 2023-08-22 Matoke Holdings Limited Antimicrobial superabsorbent compositions

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2529893A (en) 2014-09-08 2016-03-09 Geotechniquesres Ltd Apparatus and method for separating pollen and optionally microbiota from honey
CN110732177B (zh) * 2019-10-24 2021-07-13 江苏润邦食品有限公司 一种旋转式蜂蜜过滤装置
FR3107640B1 (fr) * 2020-02-28 2023-12-08 Oudin Charles Olivier Dispositif d’extraction de miel et méthode associée
FR3125677A1 (fr) * 2021-07-28 2023-02-03 Bee's Dream Pressoir a miel, dosette de miel, systeme d’extraction de miel

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Publication number Priority date Publication date Assignee Title
US6203797B1 (en) * 1998-01-06 2001-03-20 Stephen C. Perry Dietary supplement and method for use as a probiotic, for alleviating the symptons associated with irritable bowel syndrome
EP2127661A1 (fr) * 2008-05-27 2009-12-02 Nestec S.A. Probiotiques pour l'amélioration du microbiote intestinal
US20120172331A1 (en) * 2010-12-31 2012-07-05 Abbott Laboratories Methods of using human milk oligosaccharides for improving airway respiratory health
EP2684469A1 (fr) * 2012-07-13 2014-01-15 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Procédés pour renforcer et évaluer la défense naturelle du côlon contre la surcroissance de C. difficile
AU2013350328A1 (en) * 2012-11-26 2015-07-09 Thomas Julius Borody Compositions for the restoration of a fecal microbiota and methods for making and using them
US20150374761A1 (en) 2011-03-09 2015-12-31 Regents Of The University Of Minnesota Freeze dried fecal microbiota for use in fecal microbial transplantation
WO2016038348A1 (fr) 2014-09-08 2016-03-17 Geotechniquesresearch Limited Procédé d'analyse des éléments microscopiques contenus dans le miel

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203797B1 (en) * 1998-01-06 2001-03-20 Stephen C. Perry Dietary supplement and method for use as a probiotic, for alleviating the symptons associated with irritable bowel syndrome
EP2127661A1 (fr) * 2008-05-27 2009-12-02 Nestec S.A. Probiotiques pour l'amélioration du microbiote intestinal
US20120172331A1 (en) * 2010-12-31 2012-07-05 Abbott Laboratories Methods of using human milk oligosaccharides for improving airway respiratory health
US20150374761A1 (en) 2011-03-09 2015-12-31 Regents Of The University Of Minnesota Freeze dried fecal microbiota for use in fecal microbial transplantation
EP2684469A1 (fr) * 2012-07-13 2014-01-15 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Procédés pour renforcer et évaluer la défense naturelle du côlon contre la surcroissance de C. difficile
AU2013350328A1 (en) * 2012-11-26 2015-07-09 Thomas Julius Borody Compositions for the restoration of a fecal microbiota and methods for making and using them
WO2016038348A1 (fr) 2014-09-08 2016-03-17 Geotechniquesresearch Limited Procédé d'analyse des éléments microscopiques contenus dans le miel

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Title
THE BRITISH JOURNAL, February 2014 (2014-02-01)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11185080B2 (en) 2014-04-30 2021-11-30 Matoke Holdings Limited Antimicrobial compositions
US11311017B2 (en) 2014-04-30 2022-04-26 Matoke Holdings Limited Antimicrobial compositions
US11730168B2 (en) 2017-10-16 2023-08-22 Matoke Holdings Limited Antimicrobial superabsorbent compositions
RU2688354C1 (ru) * 2018-03-12 2019-05-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Способ сохранности пчел в зимний период

Also Published As

Publication number Publication date
GB2529893A (en) 2016-03-09
WO2016038348A1 (fr) 2016-03-17
GB2564514A (en) 2019-01-16
GB201805634D0 (en) 2018-05-23
GB201415818D0 (en) 2014-10-22

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