WO2017042568A1 - Health supplements - Google Patents

Health supplements Download PDF

Info

Publication number
WO2017042568A1
WO2017042568A1 PCT/GB2016/052779 GB2016052779W WO2017042568A1 WO 2017042568 A1 WO2017042568 A1 WO 2017042568A1 GB 2016052779 W GB2016052779 W GB 2016052779W WO 2017042568 A1 WO2017042568 A1 WO 2017042568A1
Authority
WO
WIPO (PCT)
Prior art keywords
honey
composition
bee
microbiota
bee feed
Prior art date
Application number
PCT/GB2016/052779
Other languages
French (fr)
Inventor
Jonah Chitolie
Original Assignee
Corbicula Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Corbicula Limited filed Critical Corbicula Limited
Priority to GB1805634.1A priority Critical patent/GB2564514A/en
Publication of WO2017042568A1 publication Critical patent/WO2017042568A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K59/00—Honey collection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Definitions

  • the present invention relates to a health supplement or probiotic composition, to a method for its production and to its use in the treatment for introducing microbiota into the human gut for improving the health of a recipient and/or treating a disease. It also relates to the treatment of a gastrointestinal disorder by administration of a composition as aforesaid and to a purpose-related health supplement or probiotic composition. The invention also relates to a bee feed and to its use.
  • Faecal Microbiota Transplant FMT Faecal Microbiota Transplant
  • This is a clinical process wherein patients lacking essential Microflora needed for their gut to function properly, are treated by implanting the beneficial intestinal bacteria and yeasts from a healthy donor.
  • the main procedures for restoring gut microbiota are by enema, or by mouth in the form of a capsule containing freeze-dried material, obtained from a healthy donor.
  • FMT has been successfully been used for the treatment of gastrointestinal diseases which include colitis, constipation and irritable bowel syndrome and infection with Clostridium difficile.
  • An object of the invention is to provide an alternative, or an improved, way of introducing microbiota into the human gut.
  • honey can provide a useful vehicle for storage and/or administration of human gut microbiota e.g. a colonic microbiota.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic.
  • the invention provides a novel method of introducing and restoring healthy human gut microbiota by adding healthy human gut microbiota, donated by healthy individuals, into treated honey and plant sugars, thus forming an oral preparation, which may be in capsule form.
  • the invention provides a method of treating a gastrointestinal disorder which comprises administering to a patient suffering from such disorder a composition comprising human gut microbiota and honey or a honey mimic.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of a gastrointestinal disorder.
  • the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of any of colitis, constipation, irritable bowel syndrome and infection with Clostridium difficile.
  • the invention provides a bee feed bee feed based on fungi fortified with compounds from plants that are medicinal. Description of preferred embodiments
  • the human gut microbiota for incorporation into the above mentioned composition may be obtained via a fecal microbial transplant technique.
  • the microbiota may be an entire fecal microbiota, a treated or untreated fecal flora sample, a complete or partial fecal flora sample, a fecal flora substantially or completely purified of non-fecal floral fecal material or a partially, substantially or completely isolated or purified fecal flora which may be lyophilized, freeze-dried or frozen, or processed into a powder.
  • a crude collected stool may be filtered and/or homogenized, and then its bacterial cells may be separated (e.g., from unwanted fibrous material) by plasmapheresis, centrifugation, centrifuge, column chromatography (e.g., affinity chromatography) or immunoprecipitation (e.g., antibodies fixed to a solid surface, such as beads or a plate).
  • plasmapheresis e.g., centrifugation, centrifuge, column chromatography (e.g., affinity chromatography) or immunoprecipitation (e.g., antibodies fixed to a solid surface, such as beads or a plate).
  • column chromatography e.g., affinity chromatography
  • immunoprecipitation e.g., antibodies fixed to a solid surface, such as beads or a plate. Freeze dried fecal microbiota is described in US 2015/374761 (Sadowsky et al.).
  • the microbiota may comprise different classes of bacteria selected from the group consisting of Actinobacteria, Bacteroidia, Bacilli, Clostridia, Erysipelotrichi, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Mollicutes, and Verrucomicrobiae.
  • the composition may comprise the human gut microbiota and a carrier which is honey free from or having a reduced content of honey bee microbiota. Additionally or alternatively, it may comprise a honey mimic comprising at least one of glucose, maltose, glycerol, sucrose and fructose.
  • honey or honey mimic may be sterilized, and may be maintained in an anaerobic state.
  • Patent Application WO 2016/038348 is described a method and apparatus for the analysis of honey and there is mentioned that the results of such analysis, together with observations and analysis of the content of a beehive, provide information on what supplements may be beneficial both to bees and humans.
  • honey could be a reservoir for bacteria, yeasts and moulds. Both aerobic, spore producing anaerobic bacteria and yeast are found in honey. Sources of microorganisms in honey are from pollen, nectar, digestive tract of honey bees and the bee hive. Spore producing anaerobic bacteria are likely to be from the digestive tract of honey bees.
  • Microbiota in honey is inactive, this may be due to low water content and high concentrations of sugars. Accordingly, healthy human gut microbiota can be stored in honey for a long period of time.
  • a human health supplement comprises honey, or a honey mimic, containing human gut microbiota.
  • Other sugar solutions including glucose, maltose, glycerol, sucrose and fructose can mimic the same concentration and specific density of honey as a bacteriostatic storage medium.
  • honey may be supplied in a suitable labelled jar, it may also be supplied in capsules with a diverse range of shell ingredient, including Shellac, soya wax, beeswax, gelatine, cellulose. They may be offered with and without enteric coating protection to allow for timed release of the microbiota at a targeted area of the human intestine or not.
  • shell ingredient including Shellac, soya wax, beeswax, gelatine, cellulose.
  • They may be offered with and without enteric coating protection to allow for timed release of the microbiota at a targeted area of the human intestine or not.
  • Capsules containing honey suspension of human gut flora are stable at room temperature and have a very long shelf life.
  • Preparations, including capsules, with healthy microbiota according to the invention may be enhanced with specific bacteria to treat particular medical conditions.
  • Having a healthy and diverse gut microbiota can contribute to our physiology, metabolism, nutrition, endocrine, neurological and immune systems. Delivery of good bacteria or good gut microbiota can be made by mixing good bacteria and/or gut microbiota with honey or similar density sugar syrups as listed above, where the honey has had the honey bee microbiota removed or neutralised through sterilisation. Time-release capsules of honey containing healthy human microbiota will enable healthy microbiota to be released into the intestine. Taken regularly over a period of time will enable a healthy and diverse gut microbiota to develop which contributes to overall health and fitness.
  • the concentration of Microbiota used in supplement may be in the order of:
  • CFU colony-forming unit
  • microbiota is captured from healthy donor faeces and stored in honey under anaerobic conditions, the honey having been either heat treated and filtered to remove the honey bee microbiota or sterilized to neutralize it.
  • FMT Fecal Microbial Transplant
  • a quantity of honey is heated and filtered to remove the honey bee microbiota.
  • the microbiota is then stored in the honey under anaerobic conditions in a concentration as follows:
  • Capsules are then created having an enteric coating comprising a mixture of polymeric acid with shellac.
  • An alternative aspect of the invention relates to a bee food supplement.
  • Bees gather fungal spores in significant amounts and also collect pollen from plants that have medicinal properties.
  • a bee feed is provided based on fungi (such as Quorn) fortified with compounds from plants that are medicinal.
  • Medicinal feeds can be used to fight viral and bacterial infections as well as to supply the bee colony with essential nutrients for the development of healthy bees.
  • Using the same principle natural plant substances that deters parasites can alleviate bee colonies of parasitic mites such as Varroa.
  • Such medicinal feeds can be given to colonies any time when needed, throughout the foraging season. Since such feeds need have no sugars they will not affect the flavour of honey produced.
  • Chytrids are the only organisms that can produce enzymes that breaks down sporopollenin. Chytrids are susceptible to pesticides especially fungicides. Areas which fungicides are heavily used therefore may lack large numbers of Chytrids which directly affect the amount of p-coumaric acid produced from the breakdown of sporopollenin.
  • honey bees gather contains many compounds that are essential to the general health and fitness of the honey bee colony.
  • Honey bees collect pollen from many medicinal herbs and plants which may prevent specific diseases and parasites.
  • Honey bees collect fungi which are associated with pollen breakdown by breaking down the inner wall of pollen and pollen contents.
  • 2016/038348 recovers pollen at different stages of breakdown. It has been observed that a type of fungi (Chytrids) are sometimes found growing on pollen.
  • Varroa destructor One of the most devastating parasites which have become widespread in bee hives is Varroa destructor.
  • a bee supplement can be made from pollen from plants which has insecticidal properties as described above.
  • Neem plant Azadirachta indica
  • Pollen from Neem can be broken down to provide a concentration of insecticide which may not affect the honey bee but can deter the feeding of Varroa and hence reduce/remove the parasite from bee hives.
  • Example 2 honey bee supplement
  • a honey bee supplement is made by choosing plants which contain specific compounds known for medicinal benefits.
  • An example is St Johns Wort (Hypericum perforatum ⁇ which contains the compound hypericin known for antiviral properties.
  • Anthers of St Johns wort are gathered in a container and crushed with a glass rod. Water is added and washed through a 150 micron sieve mesh as described in Patent Application WO 2016/038348 to remove anthers and large plant matter.
  • the filtered liquid containing pollen is collected and washed through a 10 micron sieve mesh as described in Patent Application WO 2016/038348.
  • the solution is mixed with mycoprotein (Quorn) to produce small patties and placed in the hive, this can be carried out any time of the year and will not affect the honey quality.
  • All products of breakdown can be mixed with Quorn to produce patties, or can be mixed with a sugar feed mainly before winter.
  • Another way of introducing a liquid supplement is to mix with a sugar feed or honey. This is fed to the bees after the main honey flow is over and before winter.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Birds (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A probiotic composition useful e.g. for treatment of any of colitis, constipation, irritable bowel syndrome and infection with clostridium difficile comprises gut microbiota and honey or a honey mimic. It may comprise the human gut microbiota and a carrier which is honey free from or having a reduced content of honey bee microbiota or it may comprise the human gut microbiota and a carrier which is a honey mimic comprising at least one of glucose, maltose, glycerol, sucrose and fructose. In an alternative aspect the invention provides a bee feed based on fungi fortified with compounds from plants that are medicinal. The above bee feed may be mixed with a sugar feed or honey.

Description

HEALTH SUPPLEMENTS
Field of the Invention
The present invention relates to a health supplement or probiotic composition, to a method for its production and to its use in the treatment for introducing microbiota into the human gut for improving the health of a recipient and/or treating a disease. It also relates to the treatment of a gastrointestinal disorder by administration of a composition as aforesaid and to a purpose-related health supplement or probiotic composition. The invention also relates to a bee feed and to its use.
Background to the Invention
Presently the best way of introducing microbiota into the human gut is by Faecal Microbiota Transplant FMT. This is a clinical process wherein patients lacking essential Microflora needed for their gut to function properly, are treated by implanting the beneficial intestinal bacteria and yeasts from a healthy donor. The main procedures for restoring gut microbiota are by enema, or by mouth in the form of a capsule containing freeze-dried material, obtained from a healthy donor. FMT has been successfully been used for the treatment of gastrointestinal diseases which include colitis, constipation and irritable bowel syndrome and infection with Clostridium difficile.
Over the last decade research in gut microbiota on health has risen exponentially due to mainly new technologies that have allowed large-scale analysis of the genetic and metabolic profile of microbial communities. Research have now linked Obesity, Diabetes type 2, allergies and maintaining a healthy immune system to a healthy gut microbiota.
An object of the invention is to provide an alternative, or an improved, way of introducing microbiota into the human gut. Summary of the Invention
It has now been recognized that honey can provide a useful vehicle for storage and/or administration of human gut microbiota e.g. a colonic microbiota.
In one aspect the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic.
The invention provides a novel method of introducing and restoring healthy human gut microbiota by adding healthy human gut microbiota, donated by healthy individuals, into treated honey and plant sugars, thus forming an oral preparation, which may be in capsule form.
In another aspect the invention provides a method of treating a gastrointestinal disorder which comprises administering to a patient suffering from such disorder a composition comprising human gut microbiota and honey or a honey mimic.
In a further aspect the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of a gastrointestinal disorder.
In a yet further aspect the invention provides a health supplement or probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of any of colitis, constipation, irritable bowel syndrome and infection with Clostridium difficile.
In an alternative aspect the invention provides a bee feed bee feed based on fungi fortified with compounds from plants that are medicinal. Description of preferred embodiments
Probiotic compositions and their use
The human gut microbiota for incorporation into the above mentioned composition may be obtained via a fecal microbial transplant technique.
The microbiota may be an entire fecal microbiota, a treated or untreated fecal flora sample, a complete or partial fecal flora sample, a fecal flora substantially or completely purified of non-fecal floral fecal material or a partially, substantially or completely isolated or purified fecal flora which may be lyophilized, freeze-dried or frozen, or processed into a powder. For example, a crude collected stool may be filtered and/or homogenized, and then its bacterial cells may be separated (e.g., from unwanted fibrous material) by plasmapheresis, centrifugation, centrifuge, column chromatography (e.g., affinity chromatography) or immunoprecipitation (e.g., antibodies fixed to a solid surface, such as beads or a plate). Freeze dried fecal microbiota is described in US 2015/374761 (Sadowsky et al.). The microbiota may comprise different classes of bacteria selected from the group consisting of Actinobacteria, Bacteroidia, Bacilli, Clostridia, Erysipelotrichi, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Mollicutes, and Verrucomicrobiae.
The composition may comprise the human gut microbiota and a carrier which is honey free from or having a reduced content of honey bee microbiota. Additionally or alternatively, it may comprise a honey mimic comprising at least one of glucose, maltose, glycerol, sucrose and fructose. The honey or honey mimic may be sterilized, and may be maintained in an anaerobic state.
In Patent Application WO 2016/038348 is described a method and apparatus for the analysis of honey and there is mentioned that the results of such analysis, together with observations and analysis of the content of a beehive, provide information on what supplements may be beneficial both to bees and humans.
In articles in the British Journal "Beecraft" for February, March, April and May 2014 there were set out hypotheses related to bee nutrition and health. The articles in Beecraft magazine discuss a hypothesis that honey is produced by the microbial hydrolysis of plant sugars gradually reducing water content to a very low percentage, at which microbial activity is much reduced. Water concentration is maintained by microbial condensation of glucose to form maltose. Also it is now thought that microbiota produces hydrogen peroxide which is immediately broken down to water and oxygen by enzymes. This hypothesis implies that honey is not antimicrobial but conserves microbiota and can be a reservoir for microbiota, the microbiota including bacteria, viruses, fungi, archaea and eukaryotes. These findings make it possible to introduce healthy human gut microbiota into the human gut system by taking orally a preparation, preferably a capsule, which contains honey loaded with human gut microbiota.
Of particular interest is the production of honey based health supplements for treating health conditions in humans.
Recent microbiological analysis of honey has found that honey could be a reservoir for bacteria, yeasts and moulds. Both aerobic, spore producing anaerobic bacteria and yeast are found in honey. Sources of microorganisms in honey are from pollen, nectar, digestive tract of honey bees and the bee hive. Spore producing anaerobic bacteria are likely to be from the digestive tract of honey bees.
Microbiota in honey is inactive, this may be due to low water content and high concentrations of sugars. Accordingly, healthy human gut microbiota can be stored in honey for a long period of time.
According to an aspect of the present invention a human health supplement comprises honey, or a honey mimic, containing human gut microbiota. Other sugar solutions including glucose, maltose, glycerol, sucrose and fructose can mimic the same concentration and specific density of honey as a bacteriostatic storage medium.
Although such honey may be supplied in a suitable labelled jar, it may also be supplied in capsules with a diverse range of shell ingredient, including Shellac, soya wax, beeswax, gelatine, cellulose. They may be offered with and without enteric coating protection to allow for timed release of the microbiota at a targeted area of the human intestine or not.
Capsules containing honey suspension of human gut flora are stable at room temperature and have a very long shelf life.
Preparations, including capsules, with healthy microbiota according to the invention may be enhanced with specific bacteria to treat particular medical conditions.
Having a healthy and diverse gut microbiota can contribute to our physiology, metabolism, nutrition, endocrine, neurological and immune systems. Delivery of good bacteria or good gut microbiota can be made by mixing good bacteria and/or gut microbiota with honey or similar density sugar syrups as listed above, where the honey has had the honey bee microbiota removed or neutralised through sterilisation. Time-release capsules of honey containing healthy human microbiota will enable healthy microbiota to be released into the intestine. Taken regularly over a period of time will enable a healthy and diverse gut microbiota to develop which contributes to overall health and fitness.
The concentration of Microbiota used in supplement may be in the order of:
Figure imgf000006_0001
In microbiology, a colony-forming unit (CFU) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. Viable is defined as the ability to multiply via binary fission under the controlled conditions.
The way to introduce microbiota to honey or sucrose sugar is by the pro- cess used in FMT (Fecal Microbial Transplant) technique. In this technique microbiota is captured from healthy donor faeces and stored in honey under anaerobic conditions, the honey having been either heat treated and filtered to remove the honey bee microbiota or sterilized to neutralize it. Example 1 - Human Health Supplement
Having harvested from a healthy human good gut microbiota via FMT (Fecal Microbial Transplant) technique, a quantity of honey is heated and filtered to remove the honey bee microbiota. The microbiota is then stored in the honey under anaerobic conditions in a concentration as follows:
Figure imgf000006_0002
Capsules are then created having an enteric coating comprising a mixture of polymeric acid with shellac. Bee food supplements
An alternative aspect of the invention relates to a bee food supplement. Bees gather fungal spores in significant amounts and also collect pollen from plants that have medicinal properties.
According to this second aspect of the invention therefore a bee feed is provided based on fungi (such as Quorn) fortified with compounds from plants that are medicinal.
Medicinal feeds can be used to fight viral and bacterial infections as well as to supply the bee colony with essential nutrients for the development of healthy bees. Using the same principle natural plant substances that deters parasites can alleviate bee colonies of parasitic mites such as Varroa. Such medicinal feeds can be given to colonies any time when needed, throughout the foraging season. Since such feeds need have no sugars they will not affect the flavour of honey produced.
Recent research has indicated that the compound p-coumaric acid is essential for the up-regulation of genes associated with the detoxification and immunity in honey bees, Apis mellifera. The compound p-coumaric acid is found in sporopollenin which compose the outer wall of all pollen grains. A group of microscopic fungi, Chytrids, are the only organisms that can produce enzymes that breaks down sporopollenin. Chytrids are susceptible to pesticides especially fungicides. Areas which fungicides are heavily used therefore may lack large numbers of Chytrids which directly affect the amount of p-coumaric acid produced from the breakdown of sporopollenin.
Pollen which honey bees gather contains many compounds that are essential to the general health and fitness of the honey bee colony. Honey bees collect pollen from many medicinal herbs and plants which may prevent specific diseases and parasites. Honey bees collect fungi which are associated with pollen breakdown by breaking down the inner wall of pollen and pollen contents.
Through the technique described and claimed in Patent Application WO
2016/038348 it has been found that fungal spores from rusts are also collected by bees throughout the year in significant quantities together with pollen from conifers, (Bisaccate pollen). Both fungal spores and bisaccate pollen would seem to play a significant part in bee nutrition but have received little attention hitherto. Bisaccate pollen contain higher amounts of p-coumaric acid and fungal spores provide honey bees with Mycoprotein (fungal proteins).
The technique of the invention described in Patent Application WO
2016/038348 recovers pollen at different stages of breakdown. It has been observed that a type of fungi (Chytrids) are sometimes found growing on pollen.
One of the most devastating parasites which have become widespread in bee hives is Varroa destructor. A bee supplement can be made from pollen from plants which has insecticidal properties as described above. Neem plant (Azadirachta indica), for example contains the compound azadirachtin known for having insecticidal properties. Pollen from Neem can be broken down to provide a concentration of insecticide which may not affect the honey bee but can deter the feeding of Varroa and hence reduce/remove the parasite from bee hives.
Example 2 - honey bee supplement
A honey bee supplement is made by choosing plants which contain specific compounds known for medicinal benefits. An example is St Johns Wort (Hypericum perforatum\ which contains the compound hypericin known for antiviral properties. Anthers of St Johns wort are gathered in a container and crushed with a glass rod. Water is added and washed through a 150 micron sieve mesh as described in Patent Application WO 2016/038348 to remove anthers and large plant matter. The filtered liquid containing pollen is collected and washed through a 10 micron sieve mesh as described in Patent Application WO 2016/038348.
This process is repeated using conifer cone containing bisaccate pollen. A mixture of St Johns wort pollen and bisaccate pollen is left in a container with water for two days. A small sample is taken and washed through a 10 micron sieve mesh as described in Patent Application WO 2016/038348. Using a microscope a large number of Chytrid fungi observed is an indication that pollen has been broken down to produce a solution rich in p-coumaric acid, as well as compounds such as hypericin from pollen content. Pollen from Neem is broken down to provide a concentration of insecticide. This is added to the supplement mixture to deter the feeding of Varroa and hence reduce/remove the parasite from bee hives.
The solution is mixed with mycoprotein (Quorn) to produce small patties and placed in the hive, this can be carried out any time of the year and will not affect the honey quality.
There may accordingly be three different type of feeds:
1 . Breakdown of sporopollenin (outer wall of pollen) by chytrid fungi to produce p-coumaric acid. This is for the bees' immune system. 2. Breakdown of pollen contents, by fungi and bacteria, which contains medicinal compounds, different plants contain different medicinal compounds, to produce different medicinal feeds for honey bees.
3. As 2 above but more specific to blood sucking parasite Varroa. Breakdown of pollen contents of plants which contains insecticidal compounds, if it doesn't kill the bees, it would deter feeding by Varroa.
All products of breakdown can be mixed with Quorn to produce patties, or can be mixed with a sugar feed mainly before winter.
Another way of introducing a liquid supplement is to mix with a sugar feed or honey. This is fed to the bees after the main honey flow is over and before winter.

Claims

1 . A probiotic composition comprising human gut microbiota and honey or a honey mimic.
2. The composition of claim 1 , comprising the human gut microbiota and a carrier which is honey free from or having a reduced content of honey bee microbiota.
3. The composition of claim 1 , comprising the human gut microbiota and a carrier which is a honey mimic comprising at least one of glucose, maltose, glycerol, sucrose and fructose.
4. The composition of any preceding claim, wherein the honey or honey mimic is sterilized.
5. The composition of any preceding claim, wherein the human gut microbiota has been obtained via a fecal microbial transplant technique.
6. The composition of any preceding claim, enhanced in specific bacteria to treat particular medical conditions.
7. The composition of any preceding claim, enhanced in bacteria for treatment of any of colitis, constipation, irritable bowel syndrome and infection with Clostridium difficile.
8. The composition of any preceding claim, maintained in an anaerobic state.
9. The composition of any preceding claim, which is in the form of an enema.
10. The composition of any preceding claim, which is in the form of a capsule.
1 1 . The composition of claim 10, wherein the shell ingredient of the capsule comprises shellac, soya wax, beeswax, gelatin or cellulose.
12. The composition of claim 10 or 1 1 , wherein the capsule has an enteric coating.
13. The composition of claim 12, wherein the enteric coating comprises a polymeric acid or a mixture of polymeric acid with any one of shellac, cetyl alcohol and cellulose acetate.
14. A method of treating a gastrointestinal disorder which comprises administering to a patient suffering from such disorder a composition as defined in any preceding claim.
15. A probiotic composition comprising human gut microbiota and honey or a honey mimic for use in the treatment of a gastrointestinal disorder.
16. The composition of claim 15, for use in the treatment of any of colitis, constipation, irritable bowel syndrome and infection with Clostridium difficile.
17. A bee feed based on fungi fortified with compounds from plants that are medicinal.
18. A bee feed as claimed in claim 17 and wherein the fungi comprise quorn.
19. A bee feed as claimed in claim 17 or claim 18 and containing p-coumaric acid.
20. A bee feed as claimed in any one of claims 17 to 19 and containing fungal spores from rusts.
21 . A bee feed as claimed in any one of claims 17 to 20 and containing bisaccate pollen.
22. A bee feed as claimed in any one of claims 17 to 21 and containing Chytrids.
23. A bee feed as claimed in any one of claims 17 to 22 and containing hypericin.
24. A bee feed as claimed in any one of claims 17 to 23 and containing azadirachtin indica.
25. A bee feed as claimed in any one of claims 17 to 18 and in the form of patties arranged for placing in a hive.
26. A bee feed as claimed in any one of claims 17 to 25 and which is mixed with a sugar feed or honey.
27. A composition as claimed in any one of claims 1 to 13 and substantially as hereinbefore described.
28. A bee feed as claimed in any one of claims 17 to 26 and substantially as hereinbefore described.
PCT/GB2016/052779 2014-09-08 2016-09-08 Health supplements WO2017042568A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1805634.1A GB2564514A (en) 2014-09-08 2016-09-08 Health supplements

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1415818.2A GB2529893A (en) 2014-09-08 2014-09-08 Apparatus and method for separating pollen and optionally microbiota from honey
PCT/GB2015/052583 WO2016038348A1 (en) 2014-09-08 2015-09-08 A method of analysing the microscopic content of honey
GBPCT/GB2015/052583 2015-09-08

Publications (1)

Publication Number Publication Date
WO2017042568A1 true WO2017042568A1 (en) 2017-03-16

Family

ID=51796327

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/GB2015/052583 WO2016038348A1 (en) 2014-09-08 2015-09-08 A method of analysing the microscopic content of honey
PCT/GB2016/052779 WO2017042568A1 (en) 2014-09-08 2016-09-08 Health supplements

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/GB2015/052583 WO2016038348A1 (en) 2014-09-08 2015-09-08 A method of analysing the microscopic content of honey

Country Status (2)

Country Link
GB (2) GB2529893A (en)
WO (2) WO2016038348A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2688354C1 (en) * 2018-03-12 2019-05-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Method of bees livability in winter period
US11185080B2 (en) 2014-04-30 2021-11-30 Matoke Holdings Limited Antimicrobial compositions
US11730168B2 (en) 2017-10-16 2023-08-22 Matoke Holdings Limited Antimicrobial superabsorbent compositions

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2529893A (en) 2014-09-08 2016-03-09 Geotechniquesres Ltd Apparatus and method for separating pollen and optionally microbiota from honey
CN110732177B (en) * 2019-10-24 2021-07-13 江苏润邦食品有限公司 Rotation type honey filter equipment
FR3107640B1 (en) * 2020-02-28 2023-12-08 Oudin Charles Olivier HONEY EXTRACTION DEVICE AND ASSOCIATED METHOD
FR3125677A1 (en) * 2021-07-28 2023-02-03 Bee's Dream HONEY PRESS, HONEY PODS, HONEY EXTRACTION SYSTEM

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203797B1 (en) * 1998-01-06 2001-03-20 Stephen C. Perry Dietary supplement and method for use as a probiotic, for alleviating the symptons associated with irritable bowel syndrome
EP2127661A1 (en) * 2008-05-27 2009-12-02 Nestec S.A. Probiotics to improve gut microbiotica
US20120172331A1 (en) * 2010-12-31 2012-07-05 Abbott Laboratories Methods of using human milk oligosaccharides for improving airway respiratory health
EP2684469A1 (en) * 2012-07-13 2014-01-15 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Methods for strengthening and assessment of the natural defence of the colon against C. difficile overgrowth
AU2013350328A1 (en) * 2012-11-26 2015-07-09 Thomas Julius Borody Compositions for the restoration of a fecal microbiota and methods for making and using them
US20150374761A1 (en) 2011-03-09 2015-12-31 Regents Of The University Of Minnesota Freeze dried fecal microbiota for use in fecal microbial transplantation
WO2016038348A1 (en) 2014-09-08 2016-03-17 Geotechniquesresearch Limited A method of analysing the microscopic content of honey

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2765072B1 (en) * 1997-06-30 1999-08-06 Joseph Bencsik DEVICES AND METHOD FOR PROVIDING A VIRGIN HONEY WITH MATCHING DIRECT FILTERING
JP3734764B2 (en) * 2002-04-17 2006-01-11 喜久二 山口 Royal jelly purification equipment
JP3675774B2 (en) * 2002-04-17 2005-07-27 喜久二 山口 Royal jelly purification equipment
CN101999571A (en) * 2010-10-22 2011-04-06 黄山市养生源蜂业有限公司 Method for processing honey
CN102830119A (en) * 2011-06-14 2012-12-19 安徽出入境检验检疫局检验检疫技术中心 Morphological method for determining main transgenic honey plant pollen in honey
CN102894245A (en) * 2012-09-29 2013-01-30 黑龙江省饶河县黑蜂园蜂业有限公司 Health-care pollen
KR101289532B1 (en) * 2013-03-08 2013-07-24 최순창 Honey filter

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203797B1 (en) * 1998-01-06 2001-03-20 Stephen C. Perry Dietary supplement and method for use as a probiotic, for alleviating the symptons associated with irritable bowel syndrome
EP2127661A1 (en) * 2008-05-27 2009-12-02 Nestec S.A. Probiotics to improve gut microbiotica
US20120172331A1 (en) * 2010-12-31 2012-07-05 Abbott Laboratories Methods of using human milk oligosaccharides for improving airway respiratory health
US20150374761A1 (en) 2011-03-09 2015-12-31 Regents Of The University Of Minnesota Freeze dried fecal microbiota for use in fecal microbial transplantation
EP2684469A1 (en) * 2012-07-13 2014-01-15 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Methods for strengthening and assessment of the natural defence of the colon against C. difficile overgrowth
AU2013350328A1 (en) * 2012-11-26 2015-07-09 Thomas Julius Borody Compositions for the restoration of a fecal microbiota and methods for making and using them
WO2016038348A1 (en) 2014-09-08 2016-03-17 Geotechniquesresearch Limited A method of analysing the microscopic content of honey

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THE BRITISH JOURNAL, February 2014 (2014-02-01)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11185080B2 (en) 2014-04-30 2021-11-30 Matoke Holdings Limited Antimicrobial compositions
US11311017B2 (en) 2014-04-30 2022-04-26 Matoke Holdings Limited Antimicrobial compositions
US11730168B2 (en) 2017-10-16 2023-08-22 Matoke Holdings Limited Antimicrobial superabsorbent compositions
RU2688354C1 (en) * 2018-03-12 2019-05-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Method of bees livability in winter period

Also Published As

Publication number Publication date
GB201805634D0 (en) 2018-05-23
GB2564514A (en) 2019-01-16
WO2016038348A1 (en) 2016-03-17
GB201415818D0 (en) 2014-10-22
GB2529893A (en) 2016-03-09

Similar Documents

Publication Publication Date Title
WO2017042568A1 (en) Health supplements
US9394513B2 (en) Method for fermentation and cultivation, fermented plant extract, fermented plant extract powder, and composition containing the extract of fermented plant
Grau et al. Probiotic Enterococcus mundtii isolate protects the model insect Tribolium castaneum against Bacillus thuringiensis
CN113265350B (en) Bifidobacterium W8118 and application thereof
WO2012102668A1 (en) Method of modifying bacteria
WO2016204120A1 (en) Method for producing fermented bamboo extract and immunostimulator
TWI425915B (en) New Pseudomonas bacteria
CN105169464B (en) A kind of natural honey wound dressing and preparation method and application
JP2024019630A (en) Novel microalgae
Chanchao Bioactivity of Honey and Propolis of Tetragonula laeviceps in Thailand
WO2005077390A1 (en) Hypoglycemic agent, remedy/preventive for diabetes and proces for producing the same
CN108103029A (en) The bacteriophage of one plant of cleavable ox source Streptococcusagalactiae and its application
CN111808771A (en) Laboratory culture powder of American bacillus larvae of bees, preparation method and application thereof
JP2013158315A (en) Agent and method for controlling disease of bee by using lactic bacterium
CN116121128A (en) Bifidobacterium animalis subspecies lactis strain GOLDGUT-BB69 and application thereof
JP7401072B2 (en) Microalgae growth promoter and its manufacturing method
KR102217456B1 (en) Fermenting method for honey using microorganism, fermented honey manufactured by the same and products using thereof
JP3858054B2 (en) Foods with olive utilization and anti-H. Pylori activity
KR102420141B1 (en) Synbiotics lactobacillus manufacturing method for improved female immune activity and stability using NK cell culture medium composition
CN110387334A (en) A kind of method of fast culture antrodia mycelia
JPH10245340A (en) Tonic aphrodisiac composition
KR101335788B1 (en) Method for preparing fermented extract of Gastrodiae elata by using of drip
Muhammad et al. Isolation and diagnosis of fungi associated with beekeepers Apis melifera and its effects on some plant pathogenic fungi
JPH06217712A (en) Feed for crustacean
Abbas et al. Isolate and Diagnose Negative Bacteria That Cause Urinary Tract Inflammation and Test Their Sensitivity to Certain Antibiotics and Rheum Palmatum

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16785550

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 201805634

Country of ref document: GB

Kind code of ref document: A

Free format text: PCT FILING DATE = 20160908

WWE Wipo information: entry into national phase

Ref document number: 1805634.1

Country of ref document: GB

122 Ep: pct application non-entry in european phase

Ref document number: 16785550

Country of ref document: EP

Kind code of ref document: A1