Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D243/00—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms
  • C07D243/06—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4
  • C07D243/10—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4 condensed with carbocyclic rings or ring systems
  • C07D243/14—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines
  • C07D243/16—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines substituted in position 5 by aryl radicals
  • C07D243/18—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines substituted in position 5 by aryl radicals substituted in position 2 by nitrogen, oxygen or sulfur atoms
  • C07D243/24—Oxygen atoms
  • C07D243/26—Preparation from compounds already containing the benzodiazepine skeleton
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C259/00—Compounds containing carboxyl groups, an oxygen atom of a carboxyl group being replaced by a nitrogen atom, this nitrogen atom being further bound to an oxygen atom and not being part of nitro or nitroso groups
  • C07C259/04—Compounds containing carboxyl groups, an oxygen atom of a carboxyl group being replaced by a nitrogen atom, this nitrogen atom being further bound to an oxygen atom and not being part of nitro or nitroso groups without replacement of the other oxygen atom of the carboxyl group, e.g. hydroxamic acids
  • C07C259/10—Compounds containing carboxyl groups, an oxygen atom of a carboxyl group being replaced by a nitrogen atom, this nitrogen atom being further bound to an oxygen atom and not being part of nitro or nitroso groups without replacement of the other oxygen atom of the carboxyl group, e.g. hydroxamic acids having carbon atoms of hydroxamic groups bound to carbon atoms of six-membered aromatic rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
  • C07C271/06—Esters of carbamic acids
  • C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
  • C07C271/26—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring
  • C07C271/28—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring to a carbon atom of a non-condensed six-membered aromatic ring
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond

Definitions

• the invention relates to the synthesis of benzodiazepine derivatives.
• Benzodiazepine derivatives such as YF476 act as an antagonists at gastrin/CCK 2 receptors (Semple et al. J Med Chem 1997; 40: 331-341).
• the present invention provides a process for producing a compound of
• R 2 and R 3 are each, independently, H or Ci_ 3 aliphatic, halo, or Ci_ 3 haloaliphatic, or wherein R 2 and R 3 together with the intervening carbon atom to which they are bonded, form a carbocyclic moiety;
• L is a bond or Ci_ 3 alkylene
• R 4 is -OR5 or -SR 5, wherein R 5 is hydrogen, optionally substituted alkyl (e.g. Ci_ 6 alkyl, such as methyl), a protecting group or -C(0)R 6 , wherein R 6 is optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety;
• R 5 is hydrogen, optionally substituted alkyl (e.g. Ci_ 6 alkyl, such as methyl), a protecting group or -C(0)R 6 , wherein R 6 is optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety;
• W and X are, independently, hydrogen, halo, d_ 8 alkyl or d_ 8 alkoxy;
• rings A and B are each, independently, a monocyclic aryl or heteroaryl, optionally substituted with one or more substituents independently selected from halo, hydroxy, amino, nitro, carboxyl, carboxamido, cyano, -S0 3 H, and optionally substituted Ci_ 8 alkyl, Ci_ 8 alkoxy, Ci_ 8 alkylamino or di(Ci_ 8 alkyl)amino,
• any one or more substituent on R ⁇ ring A or ring B may be unprotected or in a protected form
• phosgene synthetic equivalent is carbonyldiimidazole (CDI), diphosgene, triphosgene, a chloroformate (e.g. 4-nitrophenyl chloroform ate) or disuccinimidyl carbonate.
• CDI carbonyldiimidazole
• diphosgene diphosgene
• triphosgene triphosgene
• chloroformate e.g. 4-nitrophenyl chloroform ate
• disuccinimidyl carbonate disuccinimidyl carbonate
• the phosgene synthetic equivalent or phosgene may, for example, be CDI .
• the process comprises the additional step of deprotection to remove one or more protecting groups wherein any one or more substituents on ring A or ring B is in a protected form.
• protecting groups may be present on any one or more substituent on ring A or ring B in the compound of formula (l-A), (l-B) or (l-C) and, in these embodiments, the process may comprise the additional step of deprotection to remove the one or more protecting groups to form a compound of formula (I), or any embodiment thereof as described herein.
• the process may comprise providing a reaction mixture by adding a compound of formula (/- A), a compound of formula (l-B) and a phosgene synthetic equivalent or phosgene to an aprotic solvent, to form a compound of formula (I) via initial formation of a protected compound (I) and the additional step of deprotection to remove one or more protecting groups to form a compound of formula (I); or providing a reaction mixture by adding a compound of formula (l-C), and a phosgene synthetic equivalent or phosgene, to an aprotic solvent and, subsequently, adding a compound of formula (l-B) to the reaction mixture to form a compound of formula (I) via initial formation of a protected compound (I) and the additional step of deprotection to remove one or more protecting groups to form a compound of formula (I)
• the process comprises step (a) of providing a reaction mixture by adding a compound of formula (l-A), a compound of formula (l-B) and a phosgene synthetic equivalent or phosgene to an aprotic solvent, to form a compound of formula (I), the reaction mixture is maintained at a temperature of no more than 50 °C, no more than 40 °C or preferably no more than 30 °C.
• the process comprises step (a) of providing a reaction mixture by adding a compound of formula (l-A), a compound of formula (l-B) and a phosgene synthetic equivalent or phosgene to an aprotic solvent, to form a compound of formula (I), the compound of formula (l-A), the compound of formula (l-B) and the phosgene synthetic equivalent or phosgene may be added to the solvent in any order. Addition of these compounds to provide a reaction mixture results in reaction in the reaction mixture to form a compound of formula (I).
• the compound of formula (l-A) and the phosgene synthetic equivalent or phosgene are added to the solvent prior to addition of the compound of formula (l-B).
• the temperature of the reaction mixture is maintained at a temperature of 0-10 °C, preferably 0-5 °C, during addition of the compound of formula (l-A) and the phosgene synthetic equivalent or phosgene to the solvent.
• the reaction mixture is preferably maintained at a temperature no more than 30 °C, for example at 15- 20 °C.
• the aprotic solvent may, for example, be dichloromethane, acetonitrile or toluene, preferably dichloromethane.
• the process comprises step (b) of providing a reaction mixture by adding a compound of formula (l-C) and a phosgene synthetic equivalent or phosgene, to an aprotic solvent and, subsequently, adding a compound of formula (l-B) to the reaction mixture, addition of these compounds results in reaction in the reaction mixture to form a compound of formula (I).
• the process of step (b) may comprise heating the reaction mixture to a temperature of at least 40 °C, preferably at least 50 °C, before addition of the compound of formula (l-B).
• the process of step (b) may, as an alternative to heating, comprise adding a non-aqueous base to the reaction before addition of the compound of formula (l-B).
• the aprotic solvent may, for example, be dichloromethane, acetonitrile or toluene, preferably acetonitrile.
• ring A is a monocyclic aryl or heteroaryl, optionally substituted with one or more substituents independently selected from halo, hydroxy, amino, nitro, carboxyl, carboxamido, cyano, - S0 3 H, and optionally substituted d_ 8 alkyl, d_ 8 alkoxy, d_ 8 alkylamino or di(d_
• R 2 and R 3 are each, independently, H or C1-.3 aliphatic, halo, or C1-.3 haloaliphatic, or wherein R 2 and R 3 together with the intervening carbon atom to which they are bonded, form a C 3 _ 6 carbocyclic moiety;
• L is a bond or C1-.3 alkylene; and
• R 4 is -OR 5 or -SR 5, wherein R 5 is hydrogen, optionally substituted alkyl (e.g.
• Ci-6 alkyl such as methyl
• a protecting group or -C(0)R 6 wherein R 6 is optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety
• R 6 is optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety
• an optionally substituted aliphatic moiety wherein any one or more substituent on may be unprotected or in a protected form.
• Any of the above embodiments of a process of the invention may, for example, be for producing a compound wherein at least one of ring A and ring B is unsubstituted or substituted phenyl or pyridyl. At least one of ring A and ring B may be unsubstituted, monosubstituted or disubstituted phenyl or unsubstituted, monosubstituted or
• Ci_8 alkyl, Ci_8 alkoxy, Ci_8 alkylamino or di(Ci_ 8 alkyl)amino the optional substituents on Ci_8 alkyl, Ci_8 alkoxy, Ci_8 alkylamino and di(Ci_ 8 alkyl)amino include any substituent as described herein for substituents on an aliphatic group, for example, halo, -N0 2 , -CN, amino, Ci_8 alkylamino, di(Ci_ 8 alkyl)amino, -S(0)H or -C0 2 H.
• ring A is phenyl having a meta substituent chosen from NHMe, NMeEt, NEt 2 , F, CI, Br, OH, OCH 3 , NH 2 , NMe 2 , N0 2 , Me, (CH 2 ) n -C0 2 H, CN, CH 2 NMe 2 , NHCHO and (CH 2 )n-S0 3 H where n is 0-2; unsubstituted phenyl or 2-, 3- or 4-pyridyl optionally with a substituent selected from F, CI, CH 3 and C0 2 H; and ring B is 2-, 3- or 4-pyridyl or phenyl.
• any one or more substituent on ring A or ring B may be unprotected or in a protected form.
• W and X may independently be H, halo, d_ 3 alkyl or d_ 3 alkoxy.
• W and X are both H.
• Any of the above embodiments may, for example, be for producing a compound wherein Ri is -CH 2 C(0)C(R 2 )(R 3 )-L-R 4 or -CH 2 CHOHC(R 2 )(R 3 )-L-R 4, preferably wherein R-i is -CH 2 C(0)C(R 2 )(R 3 )-L-R 4 .
• a process of the invention as described herein may be for producing a compound wherein is -CH 2 CHOH(CH 2 ) a R 7 or -CH 2 C(0)(CH 2 ) a R 8 in which a is 0 or 1 and R 7 and R 8 are, independently, alkyl, cycloalkyl or a saturated heterocyclic group optionally substituted at a hetero-atom.
• R 7 and R 8 are selected from Ci-8 alkyl, C 3 . 8 cycloalkyl (which may be unsubstituted or substituted with one or more Ci-8 al cyclic groups of formulae (/-a) and (/- >) :
• R 9 is H or Ci_ 3 alkyl or Ci_ 3 acyl and b is 1 or 2.
• R 7 is C 4 . 7 linear or branched alkyl and R 8 is Ci_ 7 (preferably C 4 . 7 ) linear or branched alkyl.
• a compound of formula (l-A) may be a compound of formula (ll-A) as described below.
• a compound of formula (l-B) may be a compound of formula (ll-B) as described below.
• a compound of formula (l-C) may be a compound of formula (ll-C) as described below.
• the compound of formula (I) may be a compound of formula (II):
• the compound of formula (/) is a compound of formula (//)
• the compound of formula (l-A) is a compound of formula (ll-A)
• the compound of formula (l-B) is a compound of formula -B)
• the compound of formula (l-C) is a compound of formula (ll-C).
• PG is a protecting group, preferably a Boc protecting group. It will be appreciated that, in this embodiment or any of the further embodiments thereof as described herein, a protected form of compound (//):
• step (a) or (b) as described above and the process comprises the additional step of deprotection as described above to remove PG and form a compound of formula (II).
• the carbocyclic moiety may be a C3-.4 carbocyclic moiety.
• R 2 and R 3 may each, independently, be H or Ci_ 2 alkyl and L may be a bond or Ci_ 3 alkylene. In some embodiments, R 2 and R 3 may each, independently, be Ci_ 2 alkyl and L may be Ci_ 3 alkylene. In some embodiments, R 2 and R 3 may each, independently, be H or Ci_ 2 alkyl and L may be Ci alkylene (-CH 2 -). In some embodiments, R 2 and R 3 may each, independently, be d-2 alkyl and L may be d alkylene (-CH 2 -).
• R 4 may be -OR 5 or -SR 5 wherein R 5 is hydrogen, methyl or -C(0)R 6 , wherein R 6 is optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety.
• R 6 is optionally substituted aliphatic, for example R 6 is substituted or unsubstituted d_ 6 aliphatic, preferably substituted or unsubstituted d_ 3 aliphatic, more preferably methyl.
• R 4 is -OR5 and R 5 is -C(0)R 6 .
• the compounds of formula (I), (l-B), (II) and (ll-B) contain a chiral centre at the position marked * and may exist in enantiomeric forms.
• Compounds may be provided as a racemic mixture of enantiomers, a non-racemic mixture of enantiomers or as a single enantiomer in optically pure form, for example the f?-enantiomer at *:
• the compound may be selected from:
• a (II) may be a compound of formula (III): wherein R 6 is as defined for any of the embodiments of formula (/) or (II) above.
• the compound may be a compound of formula (IV):
• Rn is selected from:
• the compound of formula (I) or (II) is a compound (TR):
• Compound (TR) contains a chiral centre and therefore exists as two enantiomers, designated (TR2) (the f?-enantiomer) and (TR3) (the S-enantiomer).
• (TR) may be provided as the racemic mixture of the enantiomers (TR2) and (TR3), a non-racemic mixture of the enantiomers (TR2) and (TR3) or as a single enantiomer (TR2 or TR3) in optically pure form.
• the racemic mixture of (TR2) and (TR3) is designated "(TR1)" herein.
• the compound of formula (/) or (II) is a compound (TR-A).
• the compound of (ll-B) is a compound where -CH 2 C(0)C(R 2 )(R 3 )-L-R 4 is - CH 2 C(0)C(Me)(Me)CH 2 -OC(0)Me.
• the compound of formula (l-A) may be a compound of formula (ll-A) and the additional step of deprotection as described above may occur to remove PG and form a compound (TR-A).
• the compound (TR-A) may be provided as the racemic mixture (TR1-A) of the enantiomers (TR2-A) (the f?-enantiomer) and (TR3-A) (the S-enantiomer), a non-racemic mixture of the enantiomers (TR2-A) and (TR3-A) or as a single enantiomer (TR2-A or TR3-A) in optically pure form.
• the compound of formula (/) or (II) may be YF476:
• the process may be for producing compound of formula (TR2-A).
• PG is a protecting group, optionally a Boc protecting group
• TR2-A The acetyl group of a compound of formula (TR2-A) may be removed to form a compound of formula (TR2).
• a Boc protecting group may be deprotected under conditions known to a person of skill in the art, for example, by exposure to a strong acid such as TFA or HCI.
• the process may be for producing a compound of formula (TR2):
• PG is a protecting group, optionally a Boc protecting group
• the invention provides a compound obtained by a process according to the first aspect of the invention.
• the invention provides a compound of formula (//-C):
• PG is a protecting group, preferably a Boc protecting group.
• An alternative process for producing a compound of formula (/), or a pharmaceutically acceptable salt thereof comprises providing a reaction mixture by adding a compound of formula (V-A), a reagent or reagents capable of rearranging the compound of formula ( V- A) to form a isocyanate intermediate of formula (V-B), and a compound of formula (l-B) to a non-aqueous solvent to form a compoun
• the reagent or reagents capable of arranging the compound of formula (V-A) to form an isocyanate intermediate of formula ( V-B) comprise a brominating agent, for example N-bromosuccinimide, and a base, for example DBU (1 ,8- diazabicyclo[5.4.0]undec-7-ene).
• the non-aqueous solvent may be an aprotic solvent, for example toluene.
• Intermediates (V-B) and (V-Bi) are formed and reacted with a compound of formula (l-B) in situ.
• the compounds of formula (l-B) and formula (/) and ring A of the compounds of formula (V-A), (V-B) and (V-Bi) are as defined above in any embodiment of the first aspect of the invention.
• 2-(2-Aminobenzoyl)pyridine may be utilised in the preparation of a compound of formula (ll-B), or embodiments thereof, as described herein.
• 2-(2-aminobenzoyl)pyridine may be prepared by a process comprising reacting morpholine with isatoic anhydride to form N- (2-aminobenzoyl)morpholine and reacting A/-(2-aminobenzoyl)morpholine with 2- lithiumpyridine to form 2-(2-aminobenzoyl)pyridine.
• 2-lithiumpyridine may be prepared by reacting 2-bromopyridine with n-butyl lithium. This process may be carried out in an aprotic solvent such as toluene.
• aliphatic means a substituted or unsubstituted straight-chain, branched or cyclic hydrocarbon, which is completely saturated or which contains one or more units of unsaturation, but which is not aromatic.
• Aliphatic groups include substituted or unsubstituted linear, branched or cyclic alkyl, alkenyl, alkynyl groups and hybrids thereof, such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
• an aliphatic group has 1 to 12, 1 to 8, 1 to 6, or 1 to 3 carbons.
• d_ 3 aliphatic encompasses straight chain and branched d_ 3 alkyl, alkenyl and alkynyl and cyclopropyl.
• heteroaliphatic means an aliphatic group in which one or more carbon atom is replaced by a heteroatom.
• heteroatom refers to nitrogen (N), oxygen (O), or sulfur (S).
• alkylene refers to a bivalent alkyl group.
• An "alkylene” is a methylene or polymethylene group, i.e. , -(CH 2 ) n -, wherein n is a positive integer.
• An alkylene may be unsubstituted or substituted.
• a substituted alkylene is an alkylene group in which one or more methylene hydrogen atoms is replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
• An alkylene chain also may be substituted at one or more positions with an aliphatic group or a substituted aliphatic group.
• the term "carbocyclic moeity" refers to a cyclic aliphatic group and includes, for example, cycloalkyl moieties.
• aryl refers to a C ⁇ 3_ 14 (preferably C 6 _io) aromatic hydrocarbon, comprising one to three rings, each of which is optionally substituted.
• Aryl groups include, without limitation, phenyl, naphthyl, and anthracenyl.
• aryl as used herein, includes groups in which an aromatic ring is fused to one or more heteroaromatic, cycloaliphatic, or heterocyclic rings, where the radical or point of attachment is on the aromatic ring.
• heteroaryl and “heteroar-” refer to an aromatic group having 5 to 14 ring atoms, preferably 5, 6, 9, or 10 ring atoms and having, in addition to carbon atoms, from one to four heteroatoms as ring atoms.
• heteroatom refers to N, O, or S.
• heteroaryl and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aromatic, cycloaliphatic, or heterocyclic rings, where the radical or point of attachment is on the heteroaromatic ring.
• halo refers to fluoro, chloro, bromo or iodo.
• haloaliphatic refers to an aliphatic moiety as defined above, substituted by one or more halo moieties.
• alkoxy refers to a -O-alkyl moiety.
• the alkyl is as defined herein and, accordingly, may optionally be substituted as defined herein for optional substituents of an aliphatic moiety.
• carboxymido refers to a -C(0)NR 2 moiety, wherein each R is, independently, H or aliphatic, preferably H.
• substituted means that a hydrogen radical of a designated moiety is replaced with the radical of a specified substituent, provided that the substitution results in a stable or chemically feasible compound.
• substituents refers to a number of substituents that equals from one to the maximum number of substituents possible based on the number of available bonding sites. Unless otherwise indicated, where multiple substituents are present, substituents may be either the same or different.
• An aryl or heteroaryl group may be optionally substituted.
• R independently, is hydrogen or an optionally substituted aliphatic, heteroaliphatic, aromatic or heteroaromatic moiety, or two occurrences of R are taken together with their intervening atom(s) to form an optionally substituted 5-7-membered aromatic, heteroaromatic, cycloaliphatic, or heterocyclic ring.
• An aliphatic or heteroaliphatic group including carbocyclic or heterocyclic rings, may be "optionally substituted".
• optional substituents on the nitrogen of a non-aromatic heterocyclic ring also include and are generally selected from
• a ring nitrogen atom of a heteroaryl or non-aromatic heterocyclic ring also may be oxidized to form the corresponding /V-hydroxy or /V-oxide compound.
• a "protected form" of a compound refers to a compound in which a functional moiety is protected by a protecting group.
• the functional moiety to be protected may be a hydroxyl, carboxyl, amino, or alkylamino moiety.
• a protected form as used herein may comprise a protected hydroxyl, protected carboxyl, or a protected amino or alkylamino moiety. Protection involves temporary blocking of the moiety so that a reaction can be carried out selectively at another reactive site in a multifunctional compound.
• a protected amino or alkyl amino may be protected by a protecting group, selected from protecting groups including, but not limited to, carbamates (including methyl, ethyl and substituted ethyl carbamates (e.g.
• Troc carbobenzyloxy
• Cbz carbobenzyloxy
• Boc te/f-butyloxycarbonyl
• Fmoc 9-fluorenylmethyloxycarbonyl
• PMB p-methoxybenzyloxycarbonyl
• DMPM 3,4-dimethoxybenzyl
• PMP p-methoxyphenyl
• Sue succinyl
• MeOSuc methoxysuccinyl
• formyl urethane protecting groups
• tosyl Ts
• other sulfonamides e.g. Nosyl & Nps.
• certain exemplary oxygen protecting groups are utilized.
• a protected hydroxyl or carboxyl may be protected by an oxygen protecting group, selected from protecting groups including, but not limited to, acetyl (Ac), benzoyl (Bz), benzyl (Bn), pivaloyl (Piv), methyl ethers, substituted methyl ethers [e.g., MOM (methoxymethyl ether), ⁇ -methoxyethoxymethyl ether (MEM), MTM (methylthiomethyl ether), BOM (benzyloxymethyl ether), p-methoxybenzyl (PMB), PMBM (p-methoxybenzyloxymethyl ether), substituted ethyl ethers, ethoxyethyl ethers, substituted benzyl ethers, methoxytrityl (MMT), tetrahydropyranyl (THP), trityl (Tr), silyl ethers (e.g., TMS (trimethylsilyl ether), TES (trieth
• aprotic solvent is used herein in accordance with standard terminology in the art to refer to a solvent which is incapable of acting as a proton donor.
• Aprotic solvents include, but are not limited to, dichloromethane, tetrahydrofuran, ethyl acetate, acetonitrile, dimethylformamide, dimethyl sulfoxide, acetone, hexane, pentane, benzene, toluene, 1 ,4-dioxane, diethyl ether, and chloroform.
• a “protic solvent” is used herein in accordance with standard terminology in the art to refer to a solvent which is capable of acting as a proton donor.
• Such a solvent has has a labile hydrogen atom bound to an oxygen or a nitrogen.
• Protic solvents include, but are not limited to, water, alcohols (e.g. methanol, ethanol, isopropyl alcohol), acetic acid, formic acid, hydrogen fluoride, and ammonia.
• a "phosgene synthetic equivalent" used in a process of the invention may, for example, be carbonyldiimidazole, diphosgene, triphosgene, a chloroformate e.g 4-nitrophenyl chloroformate or disuccinimidyl carbonate (DSC).
• a chloroformate e.g 4-nitrophenyl chloroformate or disuccinimidyl carbonate (DSC).
• a chloroformate is a compound of formula CIC(0)OR.
• R may be, for example, optionally substituted aliphatic, heteroaliphatic, aryl or heteroaryl.
• Compounds of formula (/) and (II) as described herein may be of use as CCK 2 /gastrin receptor antagonists and may be useful for the prevention and/or treatment of disorders associated with CCK 2 /gastrin receptors, disorders caused by or associated with hypergastrinaemia and gastric acid-related disorders. Such disorders include disorders associated with CCK 2 receptor-bearing cells or failure or dysfunction of a physiological function in which gastrin is involved.
• disorders that can be treated and/or prevented include, without limitation, any one or more of gastric and duodenal ulcers, non-steroid anti-inflammatory drug (NSAID)-induced gastric ulceration, dyspepsia, gastro-oesophageal reflux disease (GORD), Barrett's oesophagus,
• NSAID non-steroid anti-inflammatory drug
• GORD gastro-oesophageal reflux disease
• ZOS Zollinger-Ellison syndrome
• PPI proton pump inhibitor
• other acid-suppressant including the effects of withdrawal
• hypergastrinaemia such as bone loss, impaired bone quality and bone fractures
• gastritis including H.
• gastric carcinoids and enterochromaffin-like (ECL)-cell hyperplasia include gastric carcinoids and enterochromaffin-like (ECL)-cell hyperplasia), neuroendocrine tumours (not limited to gastric carcinoids), parietal cell hyperplasia, fundic gland polyps, gastric cancer, colorectal cancer, medullary thyroid cancer, pancreatic cancer, and small cell lung cancer.
• the compounds may also be useful for the prevention and/or treatment of disorders induced by the dysfunction of a physiological function controlled by the central or peripheral CCK 2 receptor, for example anxiety, nociception, pain, drug addiction, analgesic dependence and analgesia withdrawal reactions.
• Compounds of formula (/) and ⁇ l-B), and embodiments thereof as described herein, have at least one chiral carbon atom and may have more than one chiral carbon atom.
• the invention includes any enantiomeric form, at any level of optical purity, and mixtures thereof, both racemic and non-racemic. Accordingly, all stereoisomeric forms of the compounds disclosed herein form part of the invention.
• An optically pure form of an enantiomer as referred to herein has an enantiomeric excess (ee) of at least 90%, preferably at least 95%, more preferably at least 98%, and even more preferably at least 99%. ee may be assessed, for example, by chiral HPLC.
• the compounds disclosed herein can exist in unsolvated as well as solvated forms for example with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms.
• the compounds as described herein, their enantiomers and mixtures thereof, may be provided as the free compound or as a suitable salt or hydrate thereof. Salts should preferably be those that are pharmaceutically acceptable, and salts and hydrates can be prepared by conventional methods, such as contacting a compound of the invention with an acid or base whose counterpart ion does not interfere with the intended use of the compound.
• Examples of pharmaceutically acceptable salts include hydrohalogenates, inorganic acid salts, organic carboxylic acid salts, organic sulphonic acid salts, amino acid salt, quaternary ammonium salts, alkaline metal salts, alkaline earth metal salts and the like.
• Basic compounds may form non-toxic acid addition salts with various inorganic and organic acids, i.e., salts containing pharmacologically acceptable anions, including, but not limited to, malate, oxalate, chloride, bromide, iodide, nitrate, sulfate, bisulfate, phosphate, acid phosphate, isonicotinate, acetate, lactate, salicylate, citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate,
• Acidic compounds may form salts with various pharmacologically acceptable cations, including alkali metal or alkaline earth metal salts, particularly calcium, magnesium, sodium, lithium, zinc, potassium, and iron salts.
• alkali metal or alkaline earth metal salts particularly calcium, magnesium, sodium, lithium, zinc, potassium, and iron salts.
• Compounds that include a basic or acidic moiety may also form pharmaceutically acceptable salts with various amino acids.
• the process of the invention avoids the need for potentially explosive azide chemistry. Solely for reference purposes, a reaction scheme, in parts A and B, showing the use of azide chemistry as avoided by a process of the invention is provided below.
• Racemic mixture (7 ?” may, if desired, be resolved by chiral HPLC chromatography, for example with column: Chiralcel OD 250 mm ⁇ 20 mm, 5 ⁇ ; mode: super critical fluid (SFC); eluent: Methanol 40%, no modifier; flow: 50 mL/min and run time: 4 min.
• Example 1 Synthesis of (TR2) and (TR2-A) via fert-Butyl
• Scheme 4 illustrates synthesis of N4 via N2 and N3. Whilst exemplary reagents are illustrated in Scheme 4, it will be appreciated that these may be varied. For example, Boc in N2 to N4 may be replaced with an alternative amino protecting group, such as Fmoc, Cbz or Ac. Conversion of 3-nitroaniline to N2 could, for example, use organic bases other than triethlyamine, e.g. DIPEA.
• the methylation of N2 to N3, preferably involves use of a base (e.g. KO'Bu or NaH) and a methylating agent (e.g. DMS or Mel).
• the solvent in this step may be an aprotic solvent, preferably a polar aprotic solvent (e.g. DMF or THF). Reduction of N3 to N4 could be performed with iron metal or by hydrogenation on a catalyst such as palladium on carbon or Raney Nickel.
• the slurry was filtered and washed with water until free of ammonia.
• the product was dried in a circulated air oven at 50 °C (until a constant weight was achieved) to give a yellow/orange solid (558 g, 95% yield, 87% GC purity).
• the reaction mixture was poured into 5% brine solution (20 L) and extracted with ethyl acetate (10 L and then 5 L). The combined organic extracts were washed with 5% brine solution (5 L) and then dried over anhydrous sodium sulfate. The solution was evaporated under vacuum to give viscous oil
• Triethylamine (915 g, 9.04 mol) and 4-(dimethylamino)-pyridine (30 g, 0.25 mol) was added to a solution of 3-nitroaniline (833 g, 6.03 mol) in tetrahydrofuran (6.1 L) at room temperature. The mixture was heated to reflux then external heating turned off. A solution of di-te/f-butyldicarbonate (1448 g, 6.63 mol) in tetrahydrofuran (2.2 L) was added at such a rate to maintain reflux. The mixture was heated at reflux with external heating for a further 2 hours. TLC (elute 33% ethyl acetate in hexane) indicated that all the
• Triethylamine (30 mL) was added to a solution of te/f-butyl methyl-(3-nitrophenyl)- carbamate (500 g, 1.98 mol) in methanol (2.5 L).
• Palladium on carbon (5% w/w; Johnson Matthey type 87L paste, 50% water; 50g) was carefully added under a nitrogen atmosphere and the mixture hydrogenated using a Parr shaker at 50 psi hydrogen pressure. Hydrogen uptake was rapid and the mixture exothermed from 20 °C to 75 °C. Hydrogenation was continued for one hour after the exotherm had ended.
• the mixture was evaporated under vacuum (75 °C/ ⁇ 100 mbar) to remove most of the acetic acid.
• the 1 , 1 '-carbonyldiimidazole slowly dissolved to form a light orange solution during the addition. The solution was stirred at 0-5°C for a further hour before warming to 15-20°C and stirring for a further hour.
• a chromatography column was wet packed with 3 kg of silica gel in 79% ethyl acetate, 20% hexane and 1 % triethylamine (the triethylamine is used only
• TR2-A may be recrystallized from ethyl acetate, if required.
• a total of 3711 g (84% yield, 98.2% HPLC purity, 99.9%ee ft-isomer chiral HPLC purity) of compound TR2-A was made from about 5234 g of compound 18-A using the above method.
• TR2-A was dissolved in acetic acid saturated with hydrogen chloride at about 20 °C and stirred for about 3 hours. Most of the acetic acid was removed from the mixture under reduced pressure before dissolving the residue in water. The mixture was neutralised with sodium bicarbonate and then extracted into dichloromethane. The combined extracts were dried over anhydrous sodium sulphate and then evaporated under reduced pressure to give a glass-like oil TR2-A, which was used directly in the next step. TR2-A was dissolved in methanol. A solution of potassium carbonate in water was added and the mixture stirred at about 20 °C for about 2 hours. Most of the methanol was removed from the mixture under reduced pressure before dissolving the residue in water. The mixture was extracted into dichloromethane.
• Scheme 6 illustrates synthesis of N1 via 15 and 16. Whilst exemplary reagents are illustrated in Scheme 6, it will be appreciated that these may be varied. For example, Boc in 15 to N1 may be replaced with an alternative amino protecting group, such as Fmoc, Cbz or Ac. Conversion of methyl 3-aminobenzoate to 15 could, for example, use organic bases other than DIPEA, e.g. triethlyamine.
• the methylation of 15 to 16, preferably involves use of a base (e.g. KO'Bu or NaH) and a methylating agent (e.g. DMS or Mel).
• the solvent in this step may be an aprotic solvent, preferably a polar aprotic solvent (e.g. DMF or THF).
• Conversion of 16 to N1 may be performed with a hydroxylamine salt (e.g. an HCI or sulphate salt) or hydroxylamine solution, a base (e.g. KOH) and a protic solvent (e.g. methanol).
• a hydroxylamine salt e.g. an HCI or sulphate salt
• a base e.g. KOH
• a protic solvent e.g. methanol
• a solution of morpholine (855 g, 9.81 mol) in toluene (10 L) was stirred to 90°C while adding isatoic anhydride (1600 g, 9.81 mol) in 25 g portions over a 2.5 hour period. Carbon dioxide was rapidly evolved during the addition.
• the resulting mixture (referred to below as the morpholide solution) was stirred at 90°C for a further hour before cooling to room temperature.
• a solution of 2-bromopyridine (3580 g, 22.66 mol) in toluene (12 L) was cooled to ⁇ -60 °C under a nitrogen atmosphere.
• n-Butyl lithium (1 .6 M in hexane) 14.10 L, 22.56 mol was slowly added over a 2-hour period while maintaining the temperature at ⁇ -60 °C.
• the mixture was stirred at ⁇ -60 °C for a further 30 minutes.
• the previously prepared morpholide solution was slowly added over a 4-hour period while maintaining the temperature at ⁇ -60 °C.
• the mixture was allowed to warm slowly to room temperature while stirring overnight.
• Britton- Robinson's buffer 0.5 ml_
• the solution was agitated at ambient temperature overnight using a vial roller system, and then filtered (0.45 ⁇ pore size; without pre-saturation).
• Two aliquots 50 ⁇ _ were sampled from the filtrate and diluted with one volume of 0.1 N hydrochloric acid and methanol (1 : 1 v/v) before analysis by HPLC-UV.
• the concentration of test compound in the filtrate was quantified relative to the concentration standard.
• TR1 YF476 (TR1) (TR2-A) ((TR1)I YF476) TR1 )
• the solubility advantage of ⁇ TR1) and (TR2-A) over YF476 is especially pronounced at pH 4-6, which is the pH range of the part of the small intestine - duodenum to terminal jejunum or mid ilium - where most drug absorption takes place.
• This enhanced solubility is an indicator that (TR1), (TR2), (TR3) and (TR2-A) are likely to be more bioavailable, and therefore better drug candidates than YF476.
• TR2 crystallise
• TR3 XRPD analysis of (TR2) confirmed an amorphous state.
• YF476 is crystalline, which contributes to poor solubility and bioavailability.
• Amorphous YF476 can be used to increase bioavailability, but requires stabilization, which can be achieved as a solid dispersion on hydroxypropyl methyl cellulose by spray-drying. Formulation of (TR) (in racemic, non-racemic or
• ⁇ TR2 and ⁇ TR3 were compared with YF476 and YM022 in CCKi and CCK 2 receptor functional assays with the following assay criteria.
• cAMP cyclic adenosine monophosphate
• ⁇ TR2 and ⁇ TR3 were potent CCK 2 receptor antagonists and less potent CCK ! receptor antagonists.
• (TR2) compared favourably to YF476 and YM022: (TR2) was only about 5-fold less potent than YF476 and YM022; and although affinity of ⁇ TR2) for the CCK 2 receptor was about 5-fold lower than that of YF476, it was twice that of YM022.
• the selectivity of (TR2) for the CCK 2 receptor over the CC ⁇ receptor was 30% higher than the selectivity of YF476.
• the potency of the antagonists is expressed as IC 50 , the concentration of antagonist that causes a half-maximum inhibition of the control agonist response.
• the affinity of the antagonist for the receptor is expressed as K B , the concentration of antagonist, which would occupy 50% of the receptors at equilibrium.
• Example 7 Receptor binding screen
• (TR2) and (TR3) to bind to other cellular and nuclear receptors was tested in a panel of 80 receptors.
• the assay used radiolabeled receptor ligands (agonist or antagonist, depending on the receptor), and the ability of the test compounds to inhibit ligand binding was measured by scintillation counting. No significant receptor binding (other than CCK 2 and CCK ⁇ was found.
• the potency of (TR2) and (TR3) was tested in a sulphorhodamine-B (SRB) proliferation assay in a human gastric adenocarcinoma cell line stably transfected with the human gastrin/CCK 2 receptor gene (AGS G R).
• SRB is a fluorescent dye that binds to proteins, so cells with a high rate of protein synthesis (proliferative cells) will show high levels of fluorescence in the SRB assay.
• the gastrin fragment G 1 7 has an anti-proliferative effect on AGSGR cells. So, when treated with G 17, the cells show lower levels of fluorescence in the SRB assay.
• (TR2) and (TR3) were compared with the positive controls YF476 and YM022.
• ED 50 values for YF476, (TR2), and ⁇ TR3) were 0.012, 0.03 and 0.3 ⁇ /kg, respectively.
• ⁇ TR2 was observed to have better oral bioavailability than YF476 in the healthy subjects.
• Pentagastrin induces gastric acid secretion, and thereby increases H + concentration.
• single oral doses of 5, 25 and 100 mg of (TR2) administered in conjunction with pentagastrin infusion were observed to cause similar dose-dependent inhibition of the increase in H + concentration of gastric aspirate induced by the intravenous infusion of pentagastrin as observed for corresponding dosing of YF476 with pentagastrin infusion.
• the potency of (TR2) as a CCK 2 receptor antagonist was similar to that of YF476 in the healthy subject.
• pentagastrin infusion i.v. dose 0 ⁇ g/kg/h for 2 h.
• TR2 and TR2-A were observed to cause similar dose-dependent inhibition of the increase in H + concentration of gastric aspirate induced by the intravenous infusion of pentagastrin as observed for dosing of YF476 with pentagastrin infusion.
• 100 mg of (TR2) and 50 mg (TR2-A) caused similar inhibition of the increase in H + concentration of gastric aspirate induced by the intravenous infusion of pentagastrin as observed for dosing of 100 mg YF476 .
• the potency of ⁇ TR2) as a CCK 2 receptor antagonist was similar to that of YF476 in healthy subjects, and the potency of (TR2-A) is greater than that of both (TR2) and YF476.
• the observed results showed that (TR2) supresses the effect of pentagastrin in a dose-dependent manner, and that a lower dose of (TR2-A) than (TR2) was required for full suppression.

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