WO2016155107A1 - 一种壳聚糖复合膜的制备方法 - Google Patents
一种壳聚糖复合膜的制备方法 Download PDFInfo
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- WO2016155107A1 WO2016155107A1 PCT/CN2015/079963 CN2015079963W WO2016155107A1 WO 2016155107 A1 WO2016155107 A1 WO 2016155107A1 CN 2015079963 W CN2015079963 W CN 2015079963W WO 2016155107 A1 WO2016155107 A1 WO 2016155107A1
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- polyvinyl alcohol
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- succinic anhydride
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- C—CHEMISTRY; METALLURGY
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/041—Mixtures of macromolecular compounds
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- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
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- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
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- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
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- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
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- C08L31/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an acyloxy radical of a saturated carboxylic acid, of carbonic acid or of a haloformic acid; Compositions of derivatives of such polymers
- C08L31/06—Homopolymers or copolymers of esters of polycarboxylic acids
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- A61K49/1851—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle having a (super)(para)magnetic core coated or functionalised with an organic macromolecular compound, i.e. oligomeric, polymeric, dendrimeric organic molecule
- A61K49/1863—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle having a (super)(para)magnetic core coated or functionalised with an organic macromolecular compound, i.e. oligomeric, polymeric, dendrimeric organic molecule the organic macromolecular compound being a polysaccharide or derivative thereof, e.g. chitosan, chitin, cellulose, pectin, starch
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B29K—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES B29B, B29C OR B29D, RELATING TO MOULDING MATERIALS OR TO MATERIALS FOR MOULDS, REINFORCEMENTS, FILLERS OR PREFORMED PARTS, e.g. INSERTS
- B29K2005/00—Use of polysaccharides or derivatives as moulding material
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2329/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal, or ketal radical; Hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Derivatives of such polymer
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- D01F9/00—Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments
Definitions
- the invention relates to a preparation method of a chitosan composite membrane.
- Tissue adhesion during surgery is a common phenomenon and an inevitable pathophysiological process during postoperative healing.
- Postoperative adhesions and scar tissue formation can cause serious complications such as peritoneal adhesions, tendon adhesions, soft tissue adhesions after eyelid injury, peripheral nerve adhesions, and often hinder the normal recovery of the human body.
- Postoperative adhesions are a common phenomenon in cardiothoracic surgery, obstetrics and gynecology, and orthopedic surgery.
- the main methods to prevent adhesions so far are: drug therapy, biological therapy, improved surgery, and the use of spacers.
- Chitosan is a product of chitin deacetylation. It is a natural alkaline polysaccharide with good biocompatibility, antibacterial and hemostasis, tissue regeneration, cell adhesion and biodegradability. There are many studies on chitosan, and chitosan film or gel can effectively prevent postoperative adhesion and weaken the degree of fibrous adhesion.
- polyelectrolyte composite membranes have become an important part of new membrane materials, and have been widely used in the fields of phase separation, nanofiltration, fruit preservation and biomedicine.
- the pure chitosan membrane is brittle, degraded and lost too quickly in body fluids, and its application is limited.
- the present invention provides a method for preparing a chitosan composite film, and the composite film synthesized by the method can effectively improve the mechanical properties of the pure chitosan film and prolong the degradation time.
- Polyvinyl alcohol (PVA) has good biocompatibility and hydrophilicity and is the most common biomedical material for wound dressings and drug delivery.
- PVA has good film forming properties and good compatibility with CS. The most outstanding advantage is that PVA film has excellent mechanical properties.
- the object of the present invention is to provide a preparation method of a chitosan composite membrane, overcome the defects of the pure chitosan membrane, and improve its performance to satisfy the use as a medical anti-blocking.
- a method for preparing a chitosan composite membrane comprising the following steps:
- the modified polyvinyl alcohol is formulated into a 0.4 wt% aqueous solution, and then added dropwise to an acetic acid solution having a concentration of 0.4 wt% of chitosan to obtain a mixed solution;
- reacting polyvinyl alcohol-124 with succinic anhydride in step (1) to obtain a modified polyvinyl alcohol comprises: dissolving polyvinyl alcohol-124 in a dimethyl sulfoxide solvent to form 20 wt% of polyvinyl alcohol-124.
- the succinic anhydride is dissolved in a dimethyl sulfoxide solvent to form a 20 wt% succinic anhydride solution, according to the molar ratio of the succinic anhydride to the -OH in the polyvinyl alcohol-124 is 1:10, 1.25:10, 1.6:10, 2.0:10, 2.5:10 calculation, the succinic anhydride solution was added dropwise to the polyvinyl alcohol-124 solution, at a temperature of 75 ° C, at 800 r / min After stirring for 5 hours, the heating was stopped. After cooling to room temperature, it was slowly added dropwise to an excess of 5-10 wt% NaOH in ethanol.
- the product was precipitated, washed repeatedly with ethanol, and dried under vacuum at 50 ° C to a constant weight to obtain a pure
- the polyvinyl alcohol was modified and stored to obtain five modified polyvinyl alcohols, which were named SP1, SP2, SP3, SP4, and SP5, respectively.
- the modified polyvinyl alcohol in the step (2) is respectively formulated into a 0.4 wt% aqueous solution by using SP1, SP2, SP3, SP4, and SP5, and then respectively added dropwise to an equal mass of 0.4 wt% of the shell.
- the acetic acid solution of the polysaccharide is mixed to prepare a mixed solution.
- the modified polyvinyl alcohol is formulated into a 0.4 wt% aqueous solution, and then added dropwise to an acetic acid solution having a concentration of 0.4 wt% of chitosan to obtain a mixed solution comprising: modifying the The polyvinyl alcohol is dissolved in deionized water to prepare a 0.4 wt% aqueous solution, and the 1 wt% aqueous acetic acid solution is used as a solvent to prepare a 0.4 wt% chitosan solution, and the diameter is A 0.45 ⁇ m needle filter was filtered to remove traces of insoluble impurities, and then the aqueous solution was added dropwise to the filtered chitosan solution, and the mixed solution was stirred at a rate of 1000 r/min until homogeneous.
- Carboxylation modification of PVA not only hydrogen bonding between modified products and chitosan molecules, but also stronger ionic bond, which improves the stability and mechanical properties of the composite membrane in simulated body fluids. It has sufficient application strength to achieve anti-blocking purposes.
- the pure chitosan membrane degrades too fast, is easy to be lost from the wound site, and affects the anti-adhesion effect.
- the chitosan composite membrane of the invention since the formation of the film by polyvinyl alcohol, the degradation of chitosan is slowed and prolonged. The blocking time enhances the application effect.
- PVA and chitosan have been shown to have good biocompatibility, small molecule succinic anhydride reacts with PVA, and after removal of unreacted small molecules, the resulting product is non-toxic and harmless, biological Good compatibility, no cytotoxicity, can meet the safety requirements of implant materials in human body.
- the materials selected for the present invention are inexpensive and the reaction is not complicated, so the product cost is not too high and is easily accepted by most ordinary patients.
- 1 is an infrared spectrum diagram of a modified polyvinyl alcohol in a method for preparing a chitosan composite film of the present invention
- FIG. 2 is a nuclear magnetic resonance spectrum of a modified polyvinyl alcohol in a method for preparing a chitosan composite film of the present invention
- FIG. 3 is a graph showing the thermal weight loss curve before and after polyvinyl alcohol modification in a method for preparing a chitosan composite membrane according to the present invention, wherein a is PVA, b is SP1, and c is SP2, d. Is SP3, e is SP4, and f is SP5;
- chitosan 4 is a composite of chitosan obtained by mixing 0.4 wt% of chitosan acetic acid aqueous solution with 0.4 wt% of different ratio of modified polyvinyl alcohol aqueous solution in a method for preparing a chitosan composite membrane of the present invention; Infrared spectrum of the membrane;
- thermogravimetric curve of a chitosan composite membrane in a method for preparing a chitosan composite membrane of the present invention wherein a is CS/SP1, b is CS/SP2, c is CS/SP3, and d is CS/SP4, e is CS/SP5;
- FIG. 6 is a contact angle of a chitosan composite film in a method for preparing a chitosan composite film of the present invention
- A is a positive control group (RPMI-1640 containing 0.64% phenol).
- B was a negative control group (RPMI-1640 complete medium)
- C and D were an experimental group (RPMI-1640 medium of chitosan composite membrane CS/SP1 and CS/SP5 extract).
- the invention provides a preparation method of a chitosan composite membrane, comprising the following steps:
- the modified polyvinyl alcohol is formulated into a 0.4 wt% aqueous solution, and then added dropwise to an acetic acid solution having a concentration of 0.4 wt% of chitosan to obtain a mixed solution;
- a method for preparing a chitosan composite film comprising:
- Step 1 reacting polyvinyl alcohol-124 with succinic anhydride to obtain a modified polyvinyl alcohol
- the step may be specifically performed by dissolving polyvinyl alcohol-124 in a dimethyl sulfoxide solvent to form a 20 wt% solution of polyvinyl alcohol-124, and dissolving the succinic anhydride in a dimethyl sulfoxide solvent.
- a 20 wt% solution of succinic anhydride is formed in which the molar ratio of the succinic anhydride to the -OH in the polyvinyl alcohol-124 is 1:10, 1.25:10, 1.6:10, 2.0:10, 2.5: 10 calculation, the succinic anhydride solution was added dropwise to the polyvinyl alcohol-124 solution, mechanically stirred at 800 ° C for 5 hours at a temperature of 75 ° C, the heating was stopped, and after cooling to room temperature, slowly drip Adding to an excess of 5-10% by weight of NaOH in ethanol solution, the product is precipitated, washed repeatedly with ethanol, and dried under vacuum at 50 ° C to a constant weight to obtain pure modified polyvinyl alcohol and preserved to obtain 5 kinds of modified poly Vinyl alcohol, named SP1, SP2, SP3, SP4, SP5.
- Step 2 The modified polyvinyl alcohol is formulated into a 0.4 wt% aqueous solution, and then added dropwise to an acetic acid solution having a concentration of 0.4 wt% of chitosan to obtain a mixed solution;
- the step may be specifically performed by dissolving the modified polyvinyl alcohol in deionized water, preparing a 0.4 wt% aqueous solution, and using 1 wt% aqueous acetic acid as a solvent to prepare a 0.4 wt% chitosan solution. And removing a trace amount of insoluble impurities by using a 0.45 ⁇ m diameter needle filter, and then adding the aqueous solution solution dropwise to the filtered chitosan solution, and stirring the mixed solution at a speed of 1000 r/min until homogeneous.
- the modified polyvinyl alcohol was separately prepared into an aqueous solution of 0.4 wt% by using SP1, SP2, SP3, SP4, and SP5, and then separately added to an acetic acid solution of an equal mass of 0.4w% chitosan to be mixed. liquid.
- Step 3 adjusting the pH of the mixed solution to 5.5 with a 0.01 wt% NaOH solution, and removing the surface bubbles after standing for 1 hour to obtain a casting solution;
- Step 4 Pour the casting liquid into a Petri dish, and dry the Petri dish in an oven at 60 ° C to a constant weight to obtain a chitosan composite film.
- an embodiment or “an embodiment” as used herein means that it may be included in Particular features, structures, or characteristics of at least one implementation of the invention.
- the polyvinyl alcohol-124 is formulated into a 20 wt% dimethyl sulfoxide solution, and then the succinic anhydride is formulated into a 25 wt% dimethyl sulfoxide solution, and the molar ratio of succinic anhydride to polyvinyl alcohol in the OH is 1 :10, 1.25:10, 1.6:10, 2.0:10, 2.5:10 calculation, the succinic anhydride solution was added dropwise to the polyvinyl alcohol solution, and mechanically stirred (800r/min) for 5 hours at a temperature of 75 °C. After the heating is stopped, after cooling to room temperature, it is slowly added dropwise to an excess of 5 to 10 wt% NaOH in ethanol. The product is precipitated, washed repeatedly with ethanol, and dried under vacuum at 50 ° C to a constant weight to obtain a pure modified poly. Vinyl alcohol and preserved.
- the modified polyvinyl alcohol was synthesized according to the formula shown in Table 1.
- the infrared characterization was carried out by Fourier transform infrared spectroscopy. The scanning range was in the range of 4000-500 cm -1 and the resolution was 4 cm -1 .
- the results are shown in Figure 1.
- . 1 is an infrared spectrum of a modified polyvinyl alcohol in a method for preparing a chitosan composite film of the present invention. As shown in FIG. 1, an absorption peak of -OH in a PVA segment occurs at 3200 to 3600 cm -1 .
- the characteristic peak at 2930 cm -1 is the asymmetric stretching vibration absorption peak of -CH 2
- the symmetric bending vibration absorption peak of -CH 2 appears at 1450 cm -1 .
- characteristic carboxylate appears at 1580cm-1 and the absorption peak of 1408cm -1, which table Mingding
- the dianhydride has successfully modified PVA.
- the sample concentration was about 10 mg/ml.
- the structure of the modified polyvinyl alcohol was tested by 1H-NMR. Please refer to FIG. 2, which is a kind of the present invention.
- the nuclear magnetic resonance spectrum of the modified polyvinyl alcohol in the preparation method of the chitosan composite film is shown in Fig. 2, ⁇ 2.50 is the solvent DMSO peak, ⁇ 3.50 is the water peak, and ⁇ 0 is the TMS peak.
- the proton peaks in PVA are: ⁇ 1.10 ⁇ 1.70 is the proton peak of CH2, ⁇ 3.70 ⁇ 3.97 is the proton peak of CH, and ⁇ 4.15 ⁇ 4.70 is OH. Proton peak.
- the proton peak of CH appears at ⁇ 4.80 ⁇ 5.20, and ⁇ 2.24 ⁇ 2.49 is the multiple peak formed by the superposition of CH 2 and CH 2 proton peaks on succinic anhydride. The appearance of the peak indicates that the succinic anhydride modification reaction can proceed smoothly.
- FIG. 3 is a graph showing the thermogravimetric curve before and after polyvinyl alcohol modification in the preparation method of the chitosan composite membrane of the present invention, as shown in FIG. 3, which shows polyvinyl alcohol and modification thereof.
- the thermogravimetric analysis results of polyvinyl alcohol, curves a, b, c, d, e, f are the thermal weight loss curves of PVA, SP1, SP2, SP3, SP4, SP5, respectively.
- the weight loss of PVA is divided into three stages: the first stage is the thermal decomposition of the PVA main chain, mainly concentrated between 250 and 400 ° C; the second stage is that the residual alkyne compound after the degradation of the PVA main chain continues to degrade into carbon and For hydrocarbons, the thermal decomposition temperature starts at 400 °C.
- the temperature at which PVA main chain thermal decomposition begins to decrease after succinic anhydride modification the decomposition quality in the second stage increases, and the thermal decomposition residual mass increases.
- chitosan (CS, degree of deacetylation 96.4%), dissolve it in 498 ml of 1.0 wt% aqueous acetic acid solution, stir well, and remove a trace of insoluble impurities by using a 0.45 ⁇ m diameter needle filter;
- the modified polyvinyl alcohol SP1 was dissolved in deionized water to prepare 500 ml of 0.4 wt% SP1 aqueous solution; 50 g of 0.4 wt% SP1 solution was added dropwise to the filtered 50 g of 0.4 wt% chitosan solution, and mixed.
- SP1, SP3, SP4, SP5 were used instead of SP1, and the other four operations were the same as above, and the other four chitosan composite membranes were prepared and named as: CS/SP2, CS/SP3, CS/SP4, CS/SP5.
- the composite film was made into a type 2 spline according to the GBT 1040.3-2006 standard, and the spline was subjected to mechanical properties test using an electronic tensile machine.
- the test conditions were: 25 ° C, RH (air humidity) 50%, and tensile speed 5 mm/min.
- FIG. 4 is a mixture of 0.4 wt% chitosan acetic acid aqueous solution and 0.4 wt% different ratio modified polyvinyl alcohol aqueous solution in a method for preparing a chitosan composite membrane according to the present invention.
- the infrared spectrum of the obtained chitosan composite film is shown in Fig. 4.
- FIG. 5 is a graph showing the thermal weight loss curve of the chitosan composite membrane in the preparation method of the chitosan composite membrane of the present invention, as shown in FIG. 5, which shows CS and SP1, SP3, and SP5.
- FIG. 6 is a contact angle of a chitosan composite film in a method for preparing a chitosan composite film according to the present invention, as shown in FIG. 6, along with a modified polyethylene in a chitosan composite film.
- the increase in the alcohol content and the increase in the water contact angle are related to the increase in the amount of polyelectrolyte complex formation and the decrease in the hydrophilic groups -NH3+, -COO-, -OH. Therefore, the hydrophilicity of the composite membrane can be controlled by changing the mass ratio of chitosan to modified polyvinyl alcohol in the composite system, thereby designing composite membranes with different hydrophilic and hydrophobic properties to meet different requirements for biofilm materials.
- the membrane was cut into a square membrane with a size of 3 cm ⁇ 3 cm, and then the membrane was immersed in a 0.9 wt% NaCl solution, and placed in a 37 ° C constant temperature shaking water bath. After 6 hours, the surface moisture was wiped off with a filter paper, and the mass was judged. When the mass is basically constant, the degree of swelling reaches equilibrium, and the equilibrium swelling degree is S0. The measurements were made in parallel 3 times and averaged. The formula for calculating the equilibrium swelling degree is as follows:
- M0 and mb are the masses of the composite film before and after swelling, respectively (g)
- FIG. 7 is an equilibrium swelling degree of the chitosan composite membrane in the preparation method of the chitosan composite membrane of the present invention in physiological saline.
- the composite membrane has a large degree of swelling.
- a large amount of positively charged -NH 3+ is distributed on the molecular chain of chitosan, and the molecular chains are mutually exclusive and easy to stretch.
- the composite membrane has a higher swelling ratio; as the SP content in the composite membrane increases, the swelling degree of the composite membrane decreases, which may be because the polyelectrolyte complex is formed as the content of the modified polyvinyl alcohol in the composite membrane increases.
- the in vitro cytotoxicity of the composite membrane was evaluated by the MTT method.
- 3T3 cells in logarithmic growth phase were seeded in 96-well culture plates at 2.4 ⁇ 104 Cell per well, placed in a 5% CO 2 incubator at 37° C. in RPMI-1640 medium (RPMI) containing 10% fetal bovine serum. -1640 complete medium) After 24 hours of culture, the medium was discarded.
- 6cm 2 different composite membrane samples (CS/SP1; CS/SP5) were autoclaved and then immersed in 1ml physiological saline (37 °C) for 6h, 12h, 24h, 48h, and the extract was RPMI-1640.
- the complete medium was diluted to 0.1 ⁇ L/ ⁇ L, and the diluted extract was used as a sample group.
- 100 ⁇ L of RPMI-1640 complete medium, RPMI-1640 medium containing 0.64% phenol, and RPMI-1640 complete medium containing complex membrane extract were added to each well as negative control group, positive control group and experimental group, respectively.
- Six replicate wells were set and cultured in a 37 ° C, 5% CO 2 incubator for 48 h, 20 ⁇ L LTTT was added to each well and cultured for 4 h. After all the crystals were dissolved, the absorbance was measured at 570 nm using an enzyme-linked immunosorbent assay, and the cell survival rate was calculated.
- Figure 8 is a photomicrograph of 3T3 cells after various conditions of culture: A: positive control group (RPMI-1640 medium containing 0.64% phenol); B: negative control group (RPMI-1640 complete medium); , D: experimental group (RPMI-1640 medium of chitosan composite membrane CS/SP1 and CS/SP5 extract).
- A positive control group (RPMI-1640 medium containing 0.64% phenol); B: negative control group (RPMI-1640 complete medium);
- D experimental group (RPMI-1640 medium of chitosan composite membrane CS/SP1 and CS/SP5 extract).
- the present invention discloses a method for preparing a chitosan composite membrane.
- the method firstly uses polyvinyl alcohol (PVA) as a raw material, and reacts with succinic anhydride to make a hydroxyl moiety of the polyvinyl alcohol main chain. Conversion to carboxyl group, preparation of modified polyvinyl alcohol (SP) with different degrees of carboxylation; then using chitosan (CS) as matrix, the modified polyvinyl alcohol and chitosan are combined by polyelectrolyte interaction, using solution CS and SP chitosan composite membranes were prepared by casting method.
- PVA polyvinyl alcohol
- SP modified polyvinyl alcohol
- CS chitosan
- the performance test results of the composite membrane showed that compared with the pure chitosan membrane, the mechanical strength of the chitosan composite membrane was improved, the elongation at break increased, and the hydrophilicity was enhanced.
- the cytotoxicity is less than grade 1, and it has potential application prospects as a medical anti-adhesive agent.
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Abstract
Description
样品名 | 丁二酸酐:聚乙烯醇OH(投料摩尔比) | 产物中OH转化成COOH(%) |
SP1 | 1.00:10 | 8 |
SP2 | 1.25:10 | 11 |
SP3 | 1.60:10 | 15 |
SP4 | 2.00:10 | 19 |
SP5 | 2.50:10 | 24 |
Claims (4)
- 一种壳聚糖复合膜的制备方法,其特征在于,该方法包括如下步骤:(1)将聚乙烯醇-124与丁二酸酐反应得到改性聚乙烯醇;(2)将所述改性聚乙烯醇配成0.4wt%水溶液,然后逐滴加入到浓度为0.4wt%壳聚糖的醋酸溶液中得到混合溶液;(3)用0.01wt%的NaOH溶液调节所述混合溶液的pH值至5.5,静置1小时后去除表面气泡,得到铸膜液;和(4)将所述铸膜液倒入培养皿中,将所述培养皿置于60℃烘箱中烘干至恒重,得到壳聚糖复合膜。
- 根据权利要求1所述的壳聚糖复合膜的制备方法,其特征在于:步骤(1)中将聚乙烯醇-124与丁二酸酐反应得到改性聚乙烯醇包括:将聚乙烯醇-124溶解在二甲亚砜溶剂中形成20wt%的聚乙烯醇-124溶液,再将丁二酸酐溶解在二甲亚砜溶剂中形成20wt%的丁二酸酐溶液,按照所述丁二酸酐与所述聚乙烯醇-124中-OH的摩尔比分别为1:10、1.25:10、1.6:10、2.0:10、2.5:10计算,将所述丁二酸酐溶液滴加至聚乙烯醇-124溶液内,在75℃的温度下,在800r/min的条件下机械搅拌5小时后停止加热,冷却至室温后,缓慢滴加到过量的含5~10wt%NaOH的乙醇溶液中,产物析出后用乙醇反复洗涤浸泡,50℃真空干燥至恒重后,得到纯净的改性聚乙烯醇并保存,得到5种改性聚乙烯醇,分别命名为SP1、SP2、SP3、SP4、SP5。
- 根据权利要求2所述的壳聚糖复合膜的制备方法,其特征在于:所述改性聚乙烯醇分别用SP1、SP2、SP3、SP4、SP5,将其分别配成0.4wt%的水溶液,然后分别滴加到等质量的0.4wt%壳聚糖的醋酸溶液混合制成混合液。
- 根据权利要求1所述的壳聚糖复合膜的制备方法,其特征在于,步骤(2)中将所述改性聚乙烯醇配成0.4wt%水溶液,然后逐滴加入到浓度为0.4wt%壳聚糖的醋酸溶液中得到混合溶液包括:将所述改性聚乙烯醇溶于去离子水,配制0.4wt%的水溶液,将1wt%醋酸 水溶液为溶剂,配制0.4wt%壳聚糖溶液,并用直径为0.45μm的针头式滤器过滤除去微量不溶杂质,然后将所述水溶液液逐滴加入到过滤后的所述壳聚糖溶液,按速度为1000r/min搅拌混合溶液至均匀。
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CN114805872A (zh) * | 2022-05-07 | 2022-07-29 | 辽宁大学 | 一种葡萄糖糖基化米糠蛋白-壳聚糖功能性复合膜及其制备方法 |
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CN115537030A (zh) * | 2022-09-27 | 2022-12-30 | 张大庆 | 一种亲水性壳聚糖复合物溶液的制备及在液态地膜中的应用 |
WO2024097919A1 (en) * | 2022-11-04 | 2024-05-10 | H.J. Heinz Company Brands Llc | Modified polyvinyl polymers and packaging for comestibles including the polymers |
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