WO2016104967A1

WO2016104967A1 - Cleansing or disinfecting composition having anti-viral activity by comprising green tea extract as active ingredient

Info

Publication number: WO2016104967A1
Application number: PCT/KR2015/012801
Authority: WO
Inventors: 신우진; 강한철
Original assignee: (주)피치켐; 홍익대학교세종캠퍼스산학협력단
Priority date: 2014-12-26
Filing date: 2015-11-26
Publication date: 2016-06-30
Also published as: KR20160079473A

Abstract

A method for producing a cleansing or disinfecting composition according to the present invention comprises the steps of: (a) producing a green tea extract from green tea leaves, green tea stems, and green tea seeds; (b) producing a green tea mixture by adding an antioxidant and a stabilizer to the green tea extract; and (c) producing a cleansing or disinfecting composition by adjusting the pH of the green tea mixture, and thus a cleansing or disinfecting composition can be produced which exhibits a strong antiviral activity as the cleansing or disinfecting composition is produced using natural material, the green tea extract, and which can be conveniently used even by a person with a skin disease as the irritation to the body is low from the reduced use of chemical substances which are added to the cleansing or disinfecting composition. Furthermore, the produced cleansing or disinfecting composition has increased shelf-life from the green tea constituent having been treated with an antioxidant and a stabilizer, thus facilitating commercialization, and has high antiviral and antibiotic activities from comprising a variety of natural additives and porous beads, thus allowing the use as a hand sanitizer for inhibiting spread and propagation of viruses, a cleanser for the body, a disinfectant for hospital rooms, or a disinfectant for animals. As such, a multi-purpose composition allowing convenient and rapid uses in various respiratory diseases, which are highly dependent on vaccines and for which the effectiveness of existing drugs is limited, can be produced.

Description

Method for preparing a cleaning or disinfecting composition having an antiviral activity including green tea extract as an active ingredient

The present invention relates to a method for preparing a cleaning or disinfecting composition having antiviral activity and long-term storage stability.

Influenza is a type of respiratory disease virus that is widespread not only in humans but also in livestock such as pigs, chickens, ducks, or horses, as well as many other types of animals.

In particular, in 2009, pandemic highly pathogenic viruses, such as the novel swine-origin influenza A (H1N1), emerged, causing massive economic losses along with numerous casualties.

The influenza virus belongs to the family Orthomyxoviridae and has eight negative stranded RNA fragments of PB2, PB1, PA, HA, NP, NA, M and NS.

In the negative strand RNA fragment, HA is a gene encoding hemagglutinin (hereinafter referred to as "HA"), which is a coat protein to be transcribed, and an antigen on the surface of a virus involved in attaching to cells of an influenza virus mammal. NA is a gene encoding neuraminidase (hereinafter referred to as "NA"), which plays an important role when a virus enters or exits a host cell, and is a mutation in the virus. By acting to produce a neuramin degrading enzyme to induce the virus is modified through the process of their antigenic alteration or antigenic alteration process is not easy to treat.

The flu-induced influenza virus has various symptoms and severity depending on the subtypes that are commonly found in winter, and it ranges from non-pathogenic to high pathogenicity with a mortality rate of 60%. Because of the high mortality rate caused by complications of pneumonia and cardiopulmonary diseases, highly pathogenic avian influenza virus is the first type of infectious disease in Korea and is classified as List A epidemic by the International Water Bureau (OIE).

In 2009, the H1N1 strain of H1N1 flu was lower than that of avian influenza, but was highly infectious and economically and socially problematic.

The avian influenza virus is a common infectious virus, the most important propagation factor is contaminated feces, and can also be transmitted by splash, air or water. In addition, avian influenza virus may be infected by humans through intermediate hosts such as pigs and chickens. In the intermediate host body, avian influenza virus causes genetic mutations or recombination with other viruses, resulting in a mutated virus. It can spread to people through infections of the respiratory tract and cause flu.

However, the development of effective preventive vaccines against newly appearing influenza viruses is insufficient and the limitation of currently used therapeutics remains a problem.

For example, ion-channel inhibitors such as Amantadine, Rimantadine, and neuraminidase (NA) inhibitor Oseltamivir, which are previously developed flu virus drugs, Janamivir (Zanamivir et al.) Has already been reported to develop a strain that is resistant to mutations and development of new antiviral intervention (urgent) is urgent.

Recently, studies have shown that the spread of the virus can be largely suppressed by personal hygiene such as wearing masks and hand washing.In addition, it is an effective method for pandemic influenza virus such as pandemic influenza because it can be quickly and easily blocked. Can be.

Accordingly, after the influenza pandemic, various hand sanitizers and hand sanitizers came out on the market as bots. Currently, KFDA manages hand sanitizers as quasi-drugs and hand sanitizers as cosmetics.

General hand sanitizers currently on the market are manufactured using ethanol, isopropanol, benzalkonium chloride, cresol, or hydrogen peroxide as the main ingredient, and propylene glycol, glycerin, and tocopherol as moisturizers to protect skin damage caused by alcohol. And gel forms containing 60 to 70% of alcohol are the most common hand sanitizers without water. In addition, the hand cleaner is used as a cosmetic aid in hand washing, hand soap, hand wash, solid hand washing soap and the like.

The above hand cleaners and hand sanitizers usually have a shelf life of 2 to 3 years, but are not recommended for use within 6 months of opening, and are not easy to be stored. Ethanol, isopropanol, benzalkonium chloride, cresol or The use of synthetic chemicals such as hydrogen peroxide, such as allergies, skin problems such as skin problems are a problem.

Accordingly, researches on natural cleaners or disinfectants using natural materials are being actively conducted. In particular, the number of research papers on various physiological activities of green tea having antiviral activity is rapidly increasing, and particularly, influenza virus, Various studies have been conducted on the preparation of cleaning agents and disinfectants using green tea extracts having antiviral activity against epidermal viruses such as retroviruses, Epsteinba virus, and herpes virus.

However, in the case of the detergent and disinfectant using the green tea extract, it is made of natural materials, and due to difficulty in shelf life and distribution, the shelf life is not easy due to the short shelf life during mass production, and the synthetic detergent is used to increase the shelf life. In the case of manufacturing the skin and allergic to the synthetic chemicals included in the prepared detergent and disinfectant, there is a problem that the use is limited.

The present invention has been made to solve the problems of the prior art as described above, by mixing antioxidants and stabilizers in green tea extract to improve the stability and shelf life of the green tea extract to improve the antiviral efficacy and It is to provide a method for preparing a cleaning or disinfecting composition having increased durability.

Another object of the present invention is to provide a technique for preventing the spread of viruses and bacteria by using the cleaning or disinfecting composition is improved by the manufacturing method of the cleaning or disinfecting composition for the purpose of disinfection and multi-purpose cleaning to improve the lifespan will be.

Another object of the present invention by using natural ingredients such as green tea extract for the preparation of cleaning or disinfecting composition shows the same or better antiviral activity and antimicrobial activity than the existing hand cleaners and multi-purpose cleaners and the amount of chemicals as a main component It is to provide a method for producing a cleaning or disinfecting composition for environmentally friendly and less harmful to human health.

The present invention to achieve the technical problem as described above, (a) preparing a green tea extract using green tea leaves, green tea stem and green tea seeds, (b) adding an antioxidant and a stabilizer to the green tea extract to the green tea mixture It proposes a method for preparing a cleaning or disinfecting composition comprising the steps of preparing and (c) adjusting the pH of the green tea mixture.

In addition, the step (a) is a method for producing a cleaning or disinfecting composition comprising the following steps:

1) taking green tea leaves, green tea stems and green tea seeds and steaming them with steam, 2) drying the green tea leaves, green tea stems and green tea seeds steamed in step 1) and drying them, 3) in step 2) Preparing green tea powder by powdering the dried green tea leaves, green tea stems and green tea seeds, 4) extracting hot water from the green tea powder to prepare hot water extract, and 5) filtering and concentrating the hot water extract to extract green tea extract. Obtaining.

In addition, the green tea extract is catechin (catechin), epicatechin (epicatechin), epigallocatechin (epigallocatechin, EGC), epicatechin gallate (epicatechin gallate, ECG), epigallocatechin gallate (EGCG) and flavonoids It is at least one compound selected from the group consisting of (flavonoid) derivatives, characterized in that it comprises 20% or less based on the total weight of the composition.

In addition, the antioxidant is at least one compound selected from the group consisting of tocopherol, ascorbic acid, ascorbic acid derivatives, gallic acid, quercetin, flavonol, flavonoids, catechol, ethoxyquine and lignan glycosides, based on the total weight of the composition As characterized in that it is included in less than 1.5%.

In addition, the stabilizer is beta-cyclodextrin (β-cyclodextrin), citric acid, magnesium chloride (magnesium chloride, MgCl2), ethylenediaminetertraacetic acid (EDTA salt), and dibutyl hydroxy toluene (BHT) It is at least one compound selected from the group consisting of, characterized in that it comprises less than 1% based on the total weight of the composition.

Further, in step (b), further mixing one or more selected from the group consisting of isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, hexanol and povidone-ioda It is characterized by.

In addition, in the step (b), it is characterized in that the porous beads, natural fragrances or natural additives are further mixed.

In addition, the cleaning or disinfecting composition is characterized by having an antiviral activity against the enveloped virus (enveloped virus).

In addition, the envelope virus is characterized in that the influenza virus (influenza virus), retrovirus (retrovirus), Epstein-barr virus (herstein simplex virus) or (herpes simplex virus).

In the method for preparing a cleaning or disinfecting composition according to the present invention, an antioxidant and a stabilizer may be mixed with green tea extract to prepare a cleaning or disinfecting composition having an increased shelf life.

In addition, the composition prepared by the method for preparing a cleaning or disinfecting composition according to the present invention can easily and quickly increase personal hygiene for various existing respiratory diseases with high vaccine dependency and limited therapeutic effect, It can greatly reduce the spread of infection, so that it can be used for the purpose of disinfection and cleaning for personal, business, hospital or livestock use. In particular, it can be used for direct disinfection of animals, farmers, barns and livestock farms.

In addition, for the preparation of cleaning or disinfecting composition, by using green tea, which is an environmentally friendly material, people with skin diseases can be easily used, and the domestic green tea industry, which has already reached the international level, can be activated.

1 is a process chart showing a method for preparing a cleaning or disinfecting composition according to the present invention.

Figure 2 is the result of confirming the antiviral activity of EGCG, the main component of green tea extract through plaque inhibition experiment.

3 is a graph showing the plaque formation experiment results of Experimental Example 2.

Hereinafter, the present invention will be described in detail.

As shown in Figure 1, the method for preparing a cleaning or disinfecting composition according to the present invention comprises the steps of: (a) preparing green tea extracts using green tea leaves, green tea stems and green tea seeds, and (b) antioxidants and stabilizers in the green tea extracts. Preparing a green tea mixture by adding (c) adjusting the pH of the green tea mixture to prepare a cleaning or disinfecting composition.

The step (a) is a step of preparing green tea extract using green tea leaves, green tea stems and green tea seeds, to prepare green tea extracts, green tea is steamed using green tea leaves and green tea stems and green tea seeds that have not been fermented. By steaming and heating to destroy the oxidase, and extracting to prepare green tea extract, it is preferable to configure so as to maintain the active ingredient of green tea.

To this end, the step (a), 1) taking green tea leaves, green tea stems and green tea seeds and steaming with steam, 2) drying the green tea leaves, green tea stems and green tea seeds steamed in step 1) and drying 3) preparing green tea powder by powdering the green tea leaves, green tea stem and green tea seeds dried in step 2), 4) extracting hot water from the green tea powder, and preparing hot water extract; and 5) Filtration after filtration of the hot water extract may be configured to obtain a green tea extract to prepare a green tea extract to maintain the active ingredients contained in the green tea leaves, green tea stem and green tea seeds.

In addition, the step 1) is preferably dried after heating to 100 to 300 ℃ to increase the content of the green tea active ingredient.

And, it is preferable to extract the hot water powder of green tea powder by heating the natural water in order to extract the hot water of step 4), the natural water is sodium (Na), calcium (Ca) having the effect of promoting osmotic pressure control and physiological action For cleaning or disinfection, containing a large amount of minerals such as magnesium (Mg), potassium (K), strontium (Sr), lithium (Li), chlorine ion (C1), zinc (Zn) and manganese (Mn) It can be configured to further improve the physical properties of the composition.

In addition, the green tea extract is prepared using the ethanol extraction method, which is not limited to the above-described method, and extracts the fermented alcohol prepared by fermenting the green tea powder, such as molasses grains or sweet potato, after distillation, as an extraction solvent. In addition, the green tea extract may be prepared by various known methods such as distillation extraction and the like, and may be used to prepare the composition.

Green tea extract prepared as described above includes catechin (catechin), epicatechin (epicatechin), epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (epigallocatechin gallate, Preparation of a cleaning or disinfecting composition comprising an antiviral active ingredient such as EGCG), a flavonoid derivative, and the like exhibits an antiviral activity that removes more than 90% of the virus to maintain a clean state.

In particular, the antiviral active ingredient contained in the green tea extract is an influenza virus (influenza virus), retrovirus (retrovirus), Epstein-barr virus (epstein-barr virus) or herpes virus (herpes simplex virus) It can effectively prevent disease caused by virus by blocking the path of virus infection by showing antiviral activity in enveloped virus), and for this purpose, cleaning or having the most desirable physical properties when green tea extract is included in 20% or less of the total weight of the composition Disinfectant compositions can be prepared.

The step (b) is to add an antioxidant and a stabilizer to the green tea extract to prepare a green tea mixture, the antioxidant can be mixed with the green tea extract for the purpose of improving the long-term storage safety of the prepared cleaning or disinfecting composition. have.

The antioxidant is a natural preservative, and the active ingredient contained in the cleaning or disinfecting composition to be described later is in contact with the oxygen in the air naturally absorbs the oxygen in the air, resulting in physical and chemical changes due to the chain reaction of free radicals. Oxidation may occur, or oxidation may be caused by a complex reaction due to temperature change, or homogeneous cleavage of water molecules may be generated by irradiation of light, and oxidation may be caused by a very strong active radical complex reaction formed thereby. The antioxidants are chemically structured so that very fast oxidation occurs so that when several oxidizable materials are present, the antioxidants are oxidized before other substances to prevent oxidation of other substances. Do not generate oxidation products such as alcohols, aldehydes, ketones, etc. W maintain the quality of the cleaning or disinfecting composition is prepared by increasing the shelf life and shows an effect of AS to maintain the antiviral activity of green tea extract.

Antioxidants which exhibit such effects are preferably mixed with antioxidants including tocopherol, ascorbic acid, ascorbyl palmitate, gallic acid, quercetin, flavonols, flavonoids, ethoxyquine, lignan glycosides and mixtures thereof. .

The antioxidant may prevent the oxidation of the components contained in the cleaning or disinfecting composition to improve shelf life and provide an effect of increasing the shelf life, and is an antioxidant of a natural material which is safer to oxidize and has an excellent antioxidant effect. It is preferable to use.

In addition, in this step, by adding an additional stabilizer in addition to the antioxidant, it may be configured to increase the antiviral activity and persistence of the green tea extract, and to further improve the long-term storage stability of the prepared composition, in particular, the stabilizer Preventing discoloration and odor of the agent serves as a result to improve the stability of the disinfecting or cleaning composition according to the present invention.

Such stabilizers include beta-cyclodextrin, citric acid, magnesium chloride (MgCl 2), ethylene diamine tertraacetic acid (EDTA salt), and dibutyl hydroxy toluene (BHT). It may be a representative example.

Meanwhile, the antioxidant may be added in an amount of 1.5% by weight or less based on the total weight of the disinfecting or cleaning composition, and the stabilizer may be included in an amount of 1% by weight or less based on the total weight of the disinfecting or cleaning composition according to the present invention.

In this step, natural preservatives other than antioxidants and stabilizers may be additionally added, and such natural preservatives may be mixed with the green tea extract with herbal or herbal extracts having antimicrobial activity. Bellflower, ginseng including saponin (saponin) component and the like, such as a representative example, such a natural preservative can further increase the shelf life of the composition prepared by inhibiting the growth of microorganisms.

In addition, in this step, isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, hexanol and povidone- in order to have additional sterilization and cleaning ability in addition to the above-mentioned green tea extract as the main cleaning ingredients It can be configured to further mix one or more selected from the group consisting of Ioda.

And, in this step it can be configured to further improve the physical properties of the composition prepared by further mixing the porous beads with green tea extract.

The above-mentioned porous beads may be chitosan, and low molecular weight chitosan may act as a preservative in a manufactured composition because it has antimicrobial and antifungal activity as a natural material, which may further increase shelf life. It exhibits excellent properties of biocompatibility to maintain skin moisturization when using disinfecting or cleaning compositions prepared by exhibiting extreme properties.

In addition, this step may be configured to give a fragrance (fragrance) to the disinfecting or cleaning composition prepared by mixing the natural fragrance with green tea extract.

Examples of the natural fragrances include rosemary, lavender, rose, lavandin, lemon, rosewood, mandarin, jasmine, and basil. ), Natural sands from plants such as sandalwood, bergamot, citronella, orange, eucalyptus, chamomile, palmarosa or majoram Extracts or essential oils may be used.

Such natural fragrances have antimicrobial ability to specific microorganisms, and when added for imparting odor, the natural fragrances can be preferably used because they exhibit an effect of simultaneously increasing antibacterial ability to the prepared disinfecting or cleaning composition.

In addition, the natural additives mixed in this step may be configured to further improve the physical properties of the disinfecting or cleaning composition prepared by further comprising a natural thickener.

At this time, the natural thickener is added to the stability of the bubbles that may be generated in the prepared cleaning or disinfecting composition, such as natural polymers such as starch, xanthan gum, locust bean gum, carrageenan, pectin gelatin The thickener of the material may be mixed and preferably mixed in an amount of 0.0001 to 10% by weight based on the total weight of the prepared cleaning or disinfecting composition, which is less than 0.0001% by weight, and has an insignificant effect. If the weight percentage is exceeded, the thickener is unstablely dispersed in the disinfecting or cleaning composition.

In addition, the step (c) is a step of preparing a disinfection or cleaning composition by adjusting the pH of the green tea mixture, the disinfection or cleaning composition may be prepared by varying the pH depending on the use, natural pH for pH adjustment As a regulating substance, the pH can be lowered using fruit acids such as grape seed extract, lemon juice, vinegar, malic acid, or citric acid, and can be configured to increase the pH using potassium hydroxide or sodium hydroxide.

At this time, the natural pH adjusting material to be added is preferably configured to adjust the pH by adding 0.01 to 0.5% by weight based on the total weight of the composition, if less than 0.01% by weight do not properly adjust the pH On the contrary, when it is used in excess of 0.5% by weight, the acidity or basicity is excessively high and the function of the composition is reduced, so it is used in a limited way, and the total pH of the disinfecting or cleaning composition prepared is hypoallergenic and healthy skin. It is preferable to configure to have a weak acidity of more than 5 less than 7 so as to maintain.

The disinfecting or cleaning composition prepared by the method for preparing a disinfecting or cleaning composition according to the present invention as described above may be prepared by formulating it in the form of a liquid, gel, emulsion, aerosol, and adding a natural additive that saponifies. It can be prepared in a solid state such as a commercially available soap by further comprising the step of mixing, for this purpose, by further adding a natural fats and oils and reacted with potassium hydroxide to form a saponification reaction with soap and natural glycerin It can be configured to induce a preparation in solid form.

Disinfecting the wards of hospitals treating patients with viruses and disinfecting hands, human bodies, clothes, and medical devices is not simple. Generally, strong acid, strong base or chlorine disinfectants are used to disinfect, which disinfects the virus effectively, but is limited to practical use.

Using the composition prepared by the method for producing a disinfecting or cleaning composition according to the present invention as a disinfectant, it is possible to directly disinfect animal or barn disinfection, livestock farmers and farmers.

And, if the composition prepared by the method for producing a disinfecting or cleaning composition according to the present invention is not harmful or harmful to human health or the environment, it can be used for direct disinfection or cleaning, and infectious coating virus present on the skin. It can remove more than 90%, exhibits antibacterial activity, effectively remove bacteria and harmful substances, and has anti-viral activity even after a long time after manufacture including antioxidants, thus producing various types of natural cleaners and disinfectants It can be applied to.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments.

The examples presented are only specific illustrations of the invention and are not intended to limit the scope of the invention.

In order to prepare a cleaning or disinfecting composition according to the present invention of Example 1, green tea extract was prepared using green tea leaves, green tea stems and green tea seeds.

Hot water extraction was used to prepare green tea extract, and the green tea leaves, green tea stems and green tea seeds were steamed and cooled, steamed and steamed in a vessel heated to 200 to 300 ° C., and then dried. Powdered with a grinder, the green tea powder 1 to 4% (w / v) is immersed in water of 70 to 90 ℃, left for 12 to 24 hours, the green tea extract is filtered and concentrated under reduced pressure An extract was prepared.

Ascorbic acid was added to the green tea extract as an antioxidant to 1 to 10 parts by weight to prepare a cleaning or disinfecting composition.

Experimental Example 1

In Experimental Example 1, a plaque formation inhibition experiment was conducted to investigate the effect of green tea extract on the activity of the flu virus.

To this end, MDCK (Madin-Darby, canine kidney, ATCC, MET) containing fetal bovine serum (FBS: GIBCO Invitrogen Coporation, USA) containing MEM (Menimum Essencial Medium: GIBCO Invitrogen Coporation, USA) a day before the experiment. USA) cell lines were inoculated at a concentration of 1.5 × 10 ⁵ cells / ml and then cultured in 6-well plates at 37 ° C., 5% CO ₂ concentration.

After 24 hours of incubation, two subtypes of influenza viruses, A / Korea / 01/09 (H1N1) and A / Aquatic Bird / w81 / 05 (H5N2), were grown on an MDCK cell line grown on the bottom of a petri dish. The infection was treated at a concentration of) / ml.

One hour after infection treatment, cells treated with virus solution were removed, and the cell lines were washed with 1 ml / well of PBS (Photassium Phosphate Buffered Saline) buffer.

The green tea extract was serially diluted twice in a final concentration from 100 μg / mL to 6.25 μg / mL and then DMEM (Dulbecco's containing trypsin enzyme at a concentration of 1% agarose, 10 μg / mL) was added to overlay media. Overlaid medium was added to Modified Eagle's Medium: GIBCO Invitrogen Coporation, USA) medium and plated onto cells for plaque formation and nothing added as a control. The overlay medium was plated by 3 ml / well of influenza virus infected cells, and after 15 minutes, the overlay medium was solidified and incubated for 72 hours in an incubator maintained at 37 ° C. 5% CO ₂ concentration.

As a result, during the 72 hours, the flu virus formed plaque, and in the experimental group to which the green tea extract of the present invention was added, the formation of plaque was inhibited. Specifically, referring to FIG. 2, which is a photograph showing a plaque inhibition test result according to Experimental Example 1, when treated with EGCG, the main component of green tea extract, it was confirmed that plaque formation of the flu virus was suppressed. As the concentration of EGCG used was increased, plaque formation was suppressed. However, it was confirmed that plaque formation was not suppressed in the control group.

In addition, after the number of plaques for each plaque inhibition experiment, the plaque formation inhibition ability of the flu virus by the green tea extract was quantified by the ratio of the number of plaques in the control group and the number of plaques in the experimental group.

Experimental Example 2

In Experimental Example 2, MEM media was added according to the manufacturer's protocol using Neoderm ^® E (TegoScience, Korea), an artificial skin cell, to confirm the virus removal ability of the green tea extract, followed by 24 hours in a 37 ° C CO ₂ incubator. After incubation, cold adaptive X-31 virus was inoculated by 5 × 10 ⁴ PFU, washed with green tea extract, and the virus removal / inactivation effect by this washing effect was verified by plaque assay in MDCK cell line. More specifically, the cells were cultured and washed with phosphate buffer saline (PBS) and the virus was instilled by 5 × 10 ⁴ PFU. After incubation for 24 hours in a 37 ℃ CO2 incubator and washed virus-infected artificial cells with 100 ㎍ / mL and 200 ㎍ / mL concentration of green tea extract or PBS, respectively. For reference, in order to confirm the virus removal ability of the green tea extract, the virus titer was confirmed by the plaque formation experiment performed in Example 1 above.

Figure 2 is a graph showing the plaque formation experiment results of Experimental Example 2.

As shown in Figure 2, the plaque was not formed in the virus-infected artificial cells in the medium washed with green tea extract, it was confirmed that the green tea extract has an excellent effect on virus removal.

Experimental Example 3

In Experimental Example 3, in order to confirm an increase in shelf life for use of the green tea extract as a hand cleaner or a disinfectant, ascorbic acid, an antioxidant, was added to the green tea extract to increase the shelf life of the green tea extract. Over time, the antiviral activity of green tea extract was confirmed.

To this end, various concentrations of ascorbic acid were added to 100 μg / mL of green tea extract and stored at room temperature for 2 months. At this time, plaque formation inhibition experiments were carried out on 200 PFU A / Korea / 01/09 (H1N1) virus once every 5 days to confirm the change in the expiration date of green tea extract.

In addition, to confirm the increase in shelf life of antioxidants and stabilizers, stabilizers were added to green tea extracts containing ascorbic acid at various concentrations and stored at room temperature for 2 months. In addition, plaque formation inhibition experiments were carried out on 200 PFU of A / Korea / 01/09 (H1N1) virus every 5 days, and the change in shelf life of green tea extracts by antioxidants and stabilizers was confirmed.

As a result of measuring the change in the expiration date of the green tea extract, it was confirmed that the antiviral activity of the green tea extract was maintained even after more than two months.

Claims

(a) preparing a green tea extract using green tea leaves, green tea stems and green tea seeds;

(b) adding an antioxidant and a stabilizer to the green tea extract to prepare a green tea mixture; And

(c) preparing a composition for washing or disinfecting by adjusting the pH of the green tea mixture.
The method of claim 1,

Step (a) is a method for preparing a cleaning or disinfecting composition comprising the following steps:

1) taking green tea leaves, green tea stems and green tea seeds and steaming them with steam;

2) drying the green tea leaves, green tea stems and green tea seeds steamed in step 1) and drying them;

3) preparing green tea powder by powdering the green tea leaves, green tea stems and green tea seeds dried in step 2);

4) preparing hot water extract by hot water extracting the green tea powder; And

5) filtering the hot water extract and concentrating to obtain a green tea extract.
The method of claim 1,

The green tea extract is catechin (catechin), epicatechin (epicatechin), epigallocatechin (epigallocatechin, EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and flavonoids (flavonoid) 1) At least one compound selected from the group consisting of derivatives, characterized in that it comprises 20% or less based on the total weight of the composition.
The method of claim 1,

The antioxidant is at least one compound selected from the group consisting of tocopherol, ascorbic acid, ascorbic acid derivatives, gallic acid, quercetin, flavonol, flavonoids, catechol, ethoxyquine and lignan glycosides, based on the total weight of the composition 1.5 Method for producing a cleaning or disinfecting composition, characterized in that less than%.
The method of claim 1,

The stabilizer is beta cyclodextrin (β-cyclodextrin), citric acid, magnesium chloride (magnesium chloride, MgCl2), ethylenediaminetertraacetic acid (EDTA salt), and dibutyl hydroxy toluene (BHT) group At least one compound selected from, and comprises a method for cleaning or disinfecting the composition, characterized in that contained in less than 1% based on the total weight of the composition.
The method of claim 1,

In the step (b), it is further characterized by mixing at least one selected from the group consisting of isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, hexanol and povidone-ioda Method for producing a cleaning or disinfecting composition.
The method of claim 1,

In the step (b), porous beads, natural fragrances or natural additives, characterized in that the mixing method for producing a composition for cleaning or disinfection.
The method of claim 1,

The cleaning or disinfecting composition is a method for preparing a cleaning or disinfecting composition, characterized in that it has an antiviral activity against the enveloped virus (enveloped virus).
The method of claim 8,

The coating virus is an influenza virus (influenza virus), retrovirus (retrovirus), Epstein-barr virus (epstein-barr virus) or herpes simplex virus (herpes simplex virus) method for producing a composition for cleaning or disinfection.