JP2009073952A - Antimicrobial cleaning composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cleaning composition which exhibits antimicrobial action. <P>SOLUTION: The antimicrobial cleaning composition comprises 0.0005-5 mass% of catechins and 0.0001-10 mass% of an anionic surfactant and has a pH of 4.5-5.5 or 7.5-8.5. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a cleaning composition that exhibits an antibacterial action.

Surfactants are widely used as components of various cleaning agents because they have an osmotic action, an emulsifying action, and a dispersing action. In recent years, in order to prevent contamination of the living environment by microorganisms, the direction of imparting antibacterial properties to various materials has increased, and in addition to the addition of antibacterial substances and antibacterial surfactants in detergents, etc. The antibacterial effect is imparted by (Non-patent Document 1).
However, there is a problem that the antibacterial effect of the antibacterial substance is not sufficiently exhibited, or there are problems such as the detergency being lowered depending on the combination of the antibacterial substance and the cleaning agent. There is a need for agents.

On the other hand, catechins are a kind of polyphenols that can be extracted from tea leaves, and are reported to be effective against food poisoning bacteria such as Staphylococcus aureus and Vibrio parahaemolyticus, drug-resistant bacteria, and plant pathogens (Patent Document 1). To 5, Non-Patent Document 2).
However, it is not known to add catechins to the detergent for the purpose of imparting antibacterial properties.
JP-A-2-276562 Japanese Patent Laid-Open No. 2-117608 JP-A-3-246227 JP-A-8-38133 JP 2000-328443 A Infection prevention Vol.9, No.5, p35-41 (1990) FFI Reports, Technical Reports “Catechin” Saneigen FFI Corporation, website (http://www.saneigenffi.co.jp/foods/index.html), September 12, 2006

  The present invention relates to providing an antibacterial cleaning agent having high safety and an excellent antibacterial action.

  As a result of examining the improvement of the antibacterial activity of the detergent, the present inventors surprisingly combined an anionic surfactant and catechins, and adjusted the pH of the mixed solution to 4.5 to 5.5 or 7 It has been found that a detergent composition exhibiting an excellent antibacterial effect can be obtained in the case of 0.5 to 8.5.

  That is, this invention contains catechins 0.0005-5 mass% and anionic surfactant 0.0001-10 mass%, and pH is 4.5-5.5 or 7.5-8.5. An antibacterial cleaning composition is provided.

  The present invention also provides cosmetic compositions, quasi-drug compositions, pharmaceutical compositions or household product compositions, (A) catechins 0.0005 to 5% by mass, and (B) anionic surfactants. Provided is a method for imparting antibacterial properties to the composition, characterized by containing 0.0001 to 10% by mass and adjusting the pH to 4.5 to 5.5 or 7.5 to 8.5. Is.

  ADVANTAGE OF THE INVENTION According to this invention, a detergent composition, a house hold product, etc. with high antibacterial property can be provided, reducing the usage-amount of catechin and anionic surfactant.

  The antibacterial detergent composition of the present invention contains catechins and an anionic surfactant.

  The catechins in the present invention are generic names for non-epimeric catechins such as catechin, catechin gallate, gallocatechin and gallocatechin gallate, and epimeric catechins such as epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin gallate. It is preferable to contain one or more of these. The catechins are preferably non-polymers.

  The catechins used in the present invention can generally be obtained by direct extraction from tea leaves or by concentrating or purifying the tea extract, but those derived from other raw materials, column purified products and chemicals It may be a synthetic product.

The tea leaves are extracted from tea leaves made from tea leaves obtained from the genus Camellia, such as C. sinensis, C. assamica, or hybrids thereof, with water, hot water, and in some cases added with an extraction aid. This can be done. Moreover, you may use together the method of extracting in so-called non-oxidative atmosphere, ventilating inert gas, such as boiling deaeration and nitrogen gas, and removing dissolved oxygen.
The tea leaves produced are: (1) Green teas such as Sencha, Bancha, Gyokuro, Tencha, Kamarocha; (2) Iron Kannon, color species, golden katsura, wushuiwa tea, etc. (3) Fermented teas such as Darjeeling, Uba, and Keyman called black tea are included.
Examples of the extraction aid include organic acids such as sodium ascorbate or organic acid salts thereof.

Concentration of the tea extract can be performed by concentrating the extract, and purification of the tea extract can be performed by purification using a solvent or a column. Various forms such as a solid, an aqueous solution, and a slurry are exemplified as the form of the concentrate or purified product of the tea extract.
For example, the tea extract (also referred to as tea catechin) is exemplified in detail in JP-A-59-219384, JP-A-4-20589, JP-A-5-260907, JP-A-5-306279, and the like. Can be prepared. Commercially available products can also be used, such as Mitsui Norin Co., Ltd. “Polyphenone”, ITO EN “Teafranc”, Taiyo Kagaku Co., Ltd. “Sunphenon”, DSM Nutritional Products “Teavigo” ", Suntory Co., Ltd." Sun Oolong "and the like.

The catechins in the tea extract are present as non-polymers or polymers, and are dissolved in the liquid, or exist as solids adsorbed or contained in a suspension of fine tea powder. .
In addition, most of the catechins in tea leaves exist as epi-catechins, and these epi-catechins can be converted to non-epi-isomers that are stereoisomers by treatment with heat, acid, alkali, etc. Can do. Accordingly, when non-epimeric catechins are used, an extract from green tea, semi-fermented tea or fermented tea or a concentrate of tea extract is made into an aqueous solution, for example, 40 to 140 ° C., 0.1 It can be obtained by heat treatment for min to 120 hours. Moreover, you may use the concentrate of the tea extract with high non-epicatechin content. They may be used alone or in combination.

Examples of the anionic surfactant in the present invention include alkyl sulfates such as sodium lauryl sulfate and potassium lauryl sulfate; alkyl ether sulfates such as polyoxyethylene lauryl sulfate triethanolamine; N-lauroyl sarcosine sodium and the like. N-acyl sarcosine salts of higher fatty acid amide sulfonates such as sodium N-myristoyl-N-methyltaurine and sodium N-stearoyl-N-methyltaurine; sodium monostearyl phosphate, sodium polyoxyethylene oleyl ether phosphate, Phosphoric acid ester salts such as sodium polyoxyethylene stearyl ether phosphate; Sulfosuccinates such as sodium di-2-ethylhexyl sulfosuccinate; Linear dodecylbenzene sulfonate sodium Alkylbenzene sulfonates such as triethanolamine salts such as linear dodecylbenzene sulfonate; monosodium N-lauroyl glutamate, monosodium N-stearoyl glutamate, disodium N-stearoyl glutamate, monosodium N-myristoyl-L-glutamate, etc. Examples thereof include N-acyl glutamate. You may use these individually or in combination of 2 or more types.
Of these, alkyl sulfate salts are preferred, and the alkyl group preferably has 12 carbon atoms.

  Salts used for anionic surfactants include alkali metal salts such as sodium and potassium: alkaline earth metal salts such as calcium and magnesium: mineral acids such as hydrochloride, sulfate, nitrate, phosphate and carbonate Acid addition salts: or acid addition salts of organic acids such as benzoic acid, sulfonate, oxalate, citrate and the like.

The pH of the antibacterial detergent composition of the present invention is such that the pH when the detergent composition is made into an aqueous solution (20 ° C.) increases the antibacterial properties of catechins and anionic surfactants from 4.5 to 5.5. Or it is preferable to set it as 7.5-8.5.
In order to bring the pH of the antibacterial composition of the present invention into such a specific range, it is preferable to incorporate a pH adjusting agent for adjusting to the above pH.
Examples of such pH adjusters include organic acid salts such as acetic acid, citric acid, fumaric acid, malic acid, lactic acid, gluconic acid and tartaric acid, inorganic salts such as phosphoric acid, hydrochloric acid and sulfuric acid, and hydroxylation such as sodium hydroxide. Products, ammonia or aqueous ammonia, ethanolamines, lower alkanolamines, arginine, basic amino acids such as lysine, etc., these may be used alone or in combination of two or more, and other pH adjusters You may combine suitably.
Moreover, the compounding quantity of a pH adjuster will not be specifically limited if it can adjust to the range of the said pH.

  As shown in the Examples below, an anionic surfactant and catechins are contained, and an excellent antibacterial effect is observed when the pH is adjusted to 4.5 to 5.5 or 7.5 to 8.5. Therefore, a composition obtained by blending these is useful as an antibacterial detergent composition. In addition, antibacterial properties can be imparted by blending antibacterial compositions obtained by blending these into cosmetics, quasi-drugs, pharmaceuticals, or household products (hereinafter sometimes simply referred to as products). .

  In the present invention, when catechins and an anionic surfactant are blended in the above composition or product, the amount of catechins contained in the composition or product effectively exhibits the antibacterial activity of the catechins and reduces the cost of use. In order to make it low, it is 0.0005-5 mass%, Preferably it is 0.001-1 mass%, More preferably, it is 0.001-0.2 mass%.

  In addition, when catechins and an anionic surfactant are blended in the above composition or product, the blending amount of the anionic surfactant in the composition or product effectively exhibits detergency while reducing rough skin. Therefore, it is 0.0001-10 mass%, Preferably, it is 0.0001-0.01 mass%.

  In addition, the mixing ratio of the catechins and the anionic surfactant does not interfere with both properties and effectively exhibits antibacterial properties and detergency, so that the catechins: anionic surfactant is 50000: 1 to 1: 20000, preferably 10,000: 1 to 1:10, more preferably 2000: 1 to 1:10.

The composition of the present invention can be produced according to a conventional method using, for example, a known solvent such as water as appropriate. Moreover, the form in particular is not restrict | limited, It can be used with forms, such as a solution, solid substance, an emulsion, a solubilization thing, a dispersion, and a gel.
The composition of the present invention thus produced can be formulated or used in any of cosmetics, quasi drugs, pharmaceuticals, or household products, such as lipsticks, milky lotions, moisturizing packs, cosmetic liquids, Cosmetics such as skin lotions: liquid preparations, gel preparations, cream preparations, poultice preparations, aerosol preparations, lotion preparations, and other skin external preparations: facial cleansers, makeup removers, skin cleansing agents such as body shampoos, hand soaps, shaving foam, shampoos, etc. Toiletries such as hair cleaners, deodorants, etc .: Oral care products such as dentifrices, mouthwashes, mouth fresheners, dentures, etc .: detergents for kitchen detergents, clothes cleaners and softeners, toilet cleaners, etc .: It can be blended in household chemical products such as insecticides, insect repellents, dehumidifiers, disinfectants, antibacterial agents, deodorants, and fragrances. In addition, sanitary products such as paper products, sanitary products, paper diapers, and medical products include, for example, spraying the composition of the present invention on paper diapers and the like, and the composition of the present invention in sheet-like paper / unemployed cloth products. It can be used by impregnating with a cleaning agent or the like. Moreover, it can mix | blend with a contact lens cleaning and preservative.
Here, the house hold product refers to products widely used in general households such as clothing, kitchen and residential detergents, soft finishes, cleaning paper products, toiletries and pet products.

  In the antibacterial cleaning composition of the present invention, other components, such as oil and fat components, bactericides, anti-inflammatory agents, preservatives, chelates, within the range that does not inhibit the effects of the present invention, depending on the form and use. Agent, moisturizer, pearlizing agent, opacifier, pigment, scrub agent, anti-acne agent, fragrance, cooling agent, dye, UV absorber, antioxidant, stabilizer, inorganic salt, quality stabilizer, polysaccharide thickener Agents, plant extracts, vitamins and the like can be blended.

  Hereinafter, the present invention will be described in more detail with reference to examples.

Test Examples 1-2 and Reference Example 1
(1) Preparation of Surfactant-Catechin Mixture The surfactant was used after being autoclaved (121 ° C., 20 minutes) and diluted to a predetermined concentration with sterilized water.
The catechin solution is prepared by dissolving a catechin preparation (POLYPHENON 70A (manufactured by Mitsui Norin Co., Ltd.)) in pure water to 10000 ppm, adjusting the pH to 6.5, diluting to a predetermined concentration, and then sterilizing by filtration ( 0.2 μm, manufactured by Kanto Chemical Co., Inc.).
In the surfactant-catechin mixture, 1 mL of the sterilized surfactant is added to 9 mL of 0.1 M phosphoric acid (pH 6.5) buffer solution, and the final concentration of catechins becomes a predetermined concentration. Thus, catechins were added and prepared. In addition, when adjusting pH (pH 5 or 8), the phosphate buffer solution (pH 5-8 vicinity) was used suitably, and it adjusted to desired pH as shown in Table 1 suitably.

(2) Contact test The target bacteria were cultured in LB medium (Becton &Dickinson; pH 7.0) for 24 hours at 30 ° C. with shaking to prepare 10 ⁷ to 10 ⁸ CFU / mL bacterial solution.
The LB medium composition consists of 1% casein pancreatic juice degradation product, 0.5% yeast extract, and 0.5% NaCl.
100 μL of the above bacterial solution prepared to 10 ⁷ to 10 ⁸ CFU / mL previously cultured in 10 mL of the surfactant-catechin mixture was added to adjust the final cell count to about 10 ⁵ CFU / mL. The culture was allowed to stand at 30 ° C. for 24 hours, and vortexed as needed.
The number of bacteria after 24 hours was determined by plating for a catechin solution without addition of a surfactant, and the presence of bacteria was confirmed by plating after a LP diluted solution of a surfactant-added catechin mixture.
For plating, 100 μL of each diluted solution was smeared on a TSA agar plate and statically cultured at 37 ° C. for 48 hours. The viable cell concentration was calculated from the number of colonies after culture.
Medium used: TSA agar plate (Tryptic Soy Agar, Becton Dickinson): 1.7% casein degradation product, 0.3% soybean enzyme degradation product, 0.25% dextrose, 0.5% sodium chloride, 1.5 % Agar, pH 7.

(3) Test bacteria
Pseudomonas aeruginosa IFO 13275

(4) Surfactant Anionic surfactant: Emal (Kao Corporation; containing 25% by mass of sodium lauryl sulfate)

  As shown in Table 1, in the vicinity of the neutral region (pH 6.5), although antibacterial properties of catechins and sodium lauryl sulfate were not recognized, in the acidic region (pH 5.0) or alkaline region (pH 8.0) Antibacterial properties were observed. Its antibacterial action was synergistic.

Claims (4)

  Antibacterial detergent composition containing 0.0005 to 5% by mass of catechins and 0.0001 to 10% by mass of anionic surfactant and having a pH of 4.5 to 5.5 or 7.5 to 8.5 object.   The antibacterial detergent composition according to claim 1, comprising a pH adjuster for adjusting the pH to 4.5 to 5.5 or 7.5 to 8.5.   The antibacterial detergent composition according to claim 1 or 2, wherein the mixing ratio of the catechins and the anionic surfactant is 50000: 1 to 1: 20000.   In cosmetic composition, quasi-drug composition, pharmaceutical composition or household product composition, (A) catechins 0.0005-5 mass%, and (B) anionic surfactant 0.0001-10 mass %, And the pH is adjusted to 4.5 to 5.5 or 7.5 to 8.5. A method for imparting antibacterial properties to the composition.