JP5571577B2 - Textile products for antiviral hygiene against envelope viruses belonging to the genus influenza A - Google Patents

Description

  The present invention relates to an antiviral sanitary textile product excellent in antiviral activity against non-enveloped viruses or enveloped viruses, such as tissues, napkins, towels, masks, sanitary clothing, antiviral filters and the like.

  More specifically, the present invention relates to non-enveloped viruses (eg, human pathogenic viruses such as Norovirus, Coxsackie virus, adenovirus, viral neuronecrosis virus (NNV), aquavirna virus (ABV), madai iridovirus (RSIV), etc. Fish disease virus, feline calicivirus which is a pathogen of feline infectious disease) or envelope virus (eg human influenza virus, avian influenza virus, herpes simplex virus type 1, Newcastle disease virus, vesicular stomatitis virus etc. Extracts of oysters that contain tannins and are highly effective against domestic pathogenic viruses and fish disease viruses such as viral hemorrhagic septic virus (oyster extract) Excellent antiviral activity by impregnating fibers with antiviral agents containing quintes, wattle tannin, pentagalloyl glucose, coffee tannin, gallic acid alkyl ester, pyrogallol, gallic acid, pentyl tannin, etc. It provides textile products for antiviral hygiene and provides means for disinfecting and preventing infection of the above viruses.

  In general, viral diseases are difficult to treat because antibiotics and the like are ineffective, and have become a major problem for animals such as humans and livestock. In recent years, SARS and avian influenza have become a hot topic, and there is a new memory, and viral diseases have become a major problem in cultured fish. There is a great demand for sanitary textile products useful for disinfection of viruses and prevention of infection.

  By the way, “Shibutsu” obtained by fermenting an extract of persimmon fruit has long been used as a Chinese medicine for lowering blood pressure in China, and has been popular as a folk medicine in Japan. This astringent astringent is rich in tannins and is said to have astringency (a property that binds to proteins and the like to tighten the tissue), as well as antibacterial and deodorizing effects. Regarding the structure and purification method of tannin (condensed tannin) contained in oysters, the following two academic papers (Matsuo and Itoo, 1981a / 1981b: Non-patent documents 1 and 2) can be referred to.

  Examples of such a composition containing persimmon astringent or persimmon tannin include, for example, an antibacterial dental composition containing a persimmon astringent extract with cyclodextrin to further improve handling and antibacterial properties (special JP 2005-232043: Patent Document 1), anticancer agent and anti-pneumonia virus agent obtained by further adding a mixture of ingredients such as peptide polysaccharide contained in Ganoderma spore (Japanese Patent Laid-Open No. 2004-331641: Patent Document 2) Has been proposed.

  JP-A-2006-206558 (Patent Document 3) describes an antibacterial composition containing a tannin substance, a fatty acid ester, a chelating agent and the like ([Claim 1]), and the tannin substance. Examples thereof include tannic acid, pyrocatechin, gallic acid, persimmon tannin, tea tannin, pentaploid tannin and the like ([Claim 4]). However, the effectiveness of this antibacterial composition has only been demonstrated against Escherichia coli and Staphylococcus aureus when tannic acid is used ([Example]). There is no specific disclosure that it has significant antiviral properties against non-enveloped viruses and is therefore extremely important as a component of antiviral compositions.

  JP-A-2006-306836 (Patent Document 4) describes that a specific phenol derivative is effective as an inactivating agent for various non-enveloped or enveloped viruses. As the phenol derivative, gallic acid alkyl ester, (N-propyl gallate, n-octyl gallate, catechin gallate, gallocatechin gallate, epicatechin gallate, etc.) are exemplified. However, in the Examples, although it has been specifically shown that n-octyl gallate has antiviral activity against herpes simplex virus type 1 (HSV-1) and vesicular stomatitis virus (VSV), Whether catechin gallate or the like contained has antiviral activity has not been demonstrated.

  Japanese Patent Application Publication No. 2004-529889 (Patent Document 5) discloses a C1-C3 alcohol (for example, ethanol) or a C2-C4 diol and an acid (a sufficient amount for adjusting the pH of the composition to 4.6 or less). For example, it is described that a composition containing an organic acid) has an inactivating action of envelope virus. However, the examples specifically show that a solution containing ethanol and hydrochloric acid, glycolic acid or succinic acid has antiviral activity against cold sores virus (HSV-1).

  Further, JP 2000-510152 A (Patent Document 6), JP 2002-505894 A (Patent Document 7), and JP 2003-511177 A (Patent Document 8) disclose organic carboxylic acids such as citric acid. An antiviral tissue paper treated with a lotion containing the above has been disclosed, and it is described that it has the effect of killing influenza viruses and rhinoviruses.

  As described above, various types of virus inactivators have been proposed, but a wide range of viruses including enveloped viruses and non-enveloped viruses are used, which are safe materials that have been ingested as foods. No product has yet been proposed that can prevent or disinfect the virus.

  In addition, the present inventors have so far used an antiviral agent against norovirus that causes food poisoning and infectious gastroenteritis using an oyster extract, and an anti-noroviral composition using the same (PCT / JP2008 / 060705). And an antiviral agent against a non-enveloped virus using an oyster extract and other specific tannins or tannin-like substances and an antiviral composition (PCT / JP2009 / 056635) using the same. Sugiyama et al. (Non-patent Document 3) describe that persimmon astringent tannin exhibits a strong anti-norovirus action and the possibility of using it as a disinfectant (ethanol preparation).

JP 2005-232043 A JP 2004-331641 A JP 2006-206558 A JP 2006-306836 A Japanese translation of PCT publication No. 2004-529889 Special Table 2000-510152 Special Table 2002-505894 Japanese translation of PCT publication No. 2003-511177

MATSUO, Tomoaki and ITOO, Saburo (1981a): Comparative Studies of Condensed Tannins from Several Young Fruits. J. Japan. Soc. Hort. Sci., 50 (2), 262-269. MATSUO, Tomoaki and ITOO, Saburo (1981b): A Simple and Rapid Purification Method of Condensed Tannins from Several Young Fruits.Agric.Biol.Chem., 45 (8), 1885-1887. Sugiyama, Hiroaki Nakai, Toru Toru, Satoshi Shimamoto, Examination of anti-norovirus action of plant-derived components and development of new ethanol preparations, Proceedings of the Annual Meeting of the Japanese Society of Food Microbiology, 2007, Vol.28th, p.56

  The present invention relates to an antiviral hygiene textile product impregnated with an antiviral agent that is excellent in inactivating action of non-enveloped virus or enveloped virus and highly safe to human body, such as tissue, napkin, towel, mask, hygiene An object of the present invention is to provide a means for easily disinfecting or preventing infection of a virus by providing clothing, an antiviral filter, and the like.

  In order to solve the above problems, the present inventors have searched for substances and plant components that are recognized as foods or food additives, that is, substances that have antiviral activity from materials that are safe to use in the mouth. However, a wide range of non-enveloped viruses or envelopes, including tannin-containing extracts of genus Oyster, catechins, wattle tannins, pentagalloyl glucose, coffee tannins, gallic acid alkyl esters, pyrogallol, gallic acid, pentaploid tannins, etc. The present invention has been completed by finding that it has a remarkable antiviral activity against viruses and that a fiber product impregnated with these active ingredients becomes an antiviral sanitary fiber product.

The outline of the present invention is as follows.
[1] At least one fibrous base material selected from the group consisting of paper, woven fabric and non-woven fabric is heated with alcohol or squeezed or extracted with a tannin-containing Diospyros plant fruit. An antiviral agent against a non-enveloped virus or an enveloped virus, comprising as an active ingredient an oyster extract processed product obtained by inactivating an enzyme derived from the plant belonging to the genus Oyster which has been contained therein Impregnated antiviral sanitary textile products.
[2] The antiviral sanitary textile product according to [1], wherein the processed product of oyster extract contains at least condensed tannin.
[3] The antiviral sanitary textile product according to [1] or [2], wherein the plant of the genus Oyster is Diospyros kaki.
[4] The antiviral agent further comprises catechins, wattle tannin, pentagalloyl glucose, coffee tannin, gallic acid alkyl ester (excluding those in the processed oyster extract), pyrogallol, gallic acid, pentaploid tannin. And at least one selected from the group consisting of organic acids and / or salts thereof (excluding those in the processed oyster extract) as an active ingredient, according to any one of [1] to [3] Antiviral sanitary textile products.
[5] The non-enveloped virus is a non-enveloped virus belonging to the genus Norovirus, Betanodavirus, Aquavirnavirus, Ranavirus, Enterovirus, Mastadenovirus, Besivirus or Rotavirus. The antiviral sanitary textile product according to any one of [1] to [4].
[6] The antivirus according to [5], which targets Norovirus, neuronecrosis virus, infectious pancreatic necrosis virus, madai iridovirus, coxsackie virus, adenovirus, feline calicivirus or rotavirus as the non-enveloped virus. Textile products for sexual hygiene.
[7] The envelope virus according to [1] to [4], wherein the envelope virus belongs to an influenza virus genus, vesiculovirus genus, simple virus genus, aburavirus genus, or nobirabrado genus. The antiviral sanitary textile product according to any one of the above.
[8] The envelope virus is a human influenza virus, avian influenza virus, vesicular stomatitis virus, herpes simplex virus type 1, Newcastle disease virus, or viral hemorrhagic sepsis virus, according to [7] Antiviral sanitary textile products.
[9] The method according to any one of [1] to [8], impregnated with an antiviral composition containing at least the antiviral agent according to any one of [1] to [8] and an alcohol. Antiviral sanitary textile products.
[10] Any one of [1] to [8], impregnated with an antiviral composition containing at least the antiviral agent according to any one of [1] to [8] and a surfactant. The antiviral sanitary textile product described.
[11] Impregnated with an antiviral composition containing at least the antiviral agent according to any one of [1] to [8] and an antibacterial agent (excluding ethanol, organic acids and salts thereof), [1] to [8] The antiviral sanitary textile product according to any one of [8].
[12] Any one of [1] to [8], impregnated with an antiviral composition containing at least the antiviral agent according to any one of [1] to [8] and a humectant. Antiviral sanitary textile products.
[13] The antiviral sanitary textile product according to any one of [9] to [12], wherein the antiviral composition further contains vitamin Cs.
[14] The antiviral composition contains an organic acid and / or a salt thereof (excluding those in the processed oyster extract) in such an amount that the pH of the composition is 6.0 to 2.0. The antiviral sanitary textile product according to any one of [9] to [13].
[15] Any of [9] to [14], wherein the oyster extract processed product (in terms of solid content) in the antiviral agent is a ratio of 0.01 to 5% by weight relative to the whole antiviral composition An antiviral sanitary textile product according to the above.
[16] The textile product for antiviral hygiene according to any one of [9] to [15], which takes the form of a tissue, wet tissue, napkin, wet napkin, towel or wet towel.
[17] The textile product for antiviral hygiene according to any one of [9] to [15], which takes the form of a mask, an auxiliary cloth in the mask, a sanitary garment, an antiviral filter or an antiviral air conditioner filter. .

  As the oyster extract of the antiviral agent impregnated in the textile product for antiviral hygiene of the present invention, the juice or extract of the fruit of the plant of the genus Oyster is heated or treated with alcohol, What is obtained by inactivating the enzyme derived from the plant belonging to the genus oysters that has been contained (treated product of oyster extract), and also contains at least condensed tannin, such as oyster (Diospyros kaki) What is obtained is preferred.

  The antiviral agent impregnated in the textile product for antiviral hygiene of the present invention includes catechins (catechin, epicatechin, epithelial) in addition to oyster extract depending on the target non-enveloped virus or envelope virus. Gallocatechin, epicatechin gallate, epigallocatechin gallate, etc.), wattle tannin, pentagalloyl glucose, coffee tannin, gallic acid alkyl ester (excluding those in oyster extract or processed product thereof), pyrogallol, gallic acid, Peptide tannins, organic acids and / or their salts (except for oyster extracts or their processed products) can also be used as active ingredients. These active ingredients of the antiviral agent may be used singly or in combination.

  Such antiviral agents are preferably impregnated into textiles as antiviral compositions in combination with alcohols, surfactants, antibacterial agents (excluding ethanol, organic acids and their salts), moisturizers, etc. It is. By doing in this way, the antiviral sanitary textile product excellent in the antiviral activity with respect to a non-enveloped virus or an envelope virus is obtained.

  It is preferable to contain the said antiviral agent (solid content conversion) in the ratio of 0.01-5 weight% with respect to the whole antiviral composition. It is also preferable to contain an organic acid and / or a salt thereof in such an amount that the pH of the composition is 6.0 to 2.0. Furthermore, it is also preferable that said antiviral composition contains vitamin Cs.

  In one embodiment, the antiviral composition contains at least the antiviral agent and alcohol, and is further impregnated into the textile product of the present invention as an alcohol preparation that may further contain water or a surfactant.

  In one embodiment, the antiviral composition is impregnated into the textile product of the present invention as a cleaning composition containing at least the antiviral agent and a surfactant.

  In one embodiment, the antiviral composition is impregnated in the textile product of the present invention as a disinfecting composition containing at least the antiviral agent and an antibacterial agent (excluding ethanol, organic acids and salts thereof). The

  In one embodiment, the antiviral composition is impregnated in the textile product of the present invention as a moisturizing composition containing at least the antiviral agent and a humectant.

  Antiviral sanitary textile products are provided for applications such as tissue, wet tissue, napkins, wet napkins, towels, wet towels, masks, auxiliary cloths in masks, sanitary clothing, antiviral filters, antiviral air conditioner filters, etc. Is done.

  Furthermore, the textile product for antiviral hygiene of the present invention is for disinfection or prevention of infection of non-enveloped viruses or enveloped viruses, such as cleaning or blocking non-enveloped viruses or places where there is a possibility of contamination by enveloped viruses. Provide the means.

  The effects on non-enveloped or enveloped viruses such as oyster extracts, catechins, wattle tannins, pentagalloyl glucose, coffee tannins, gallic acid alkyl esters, pyrogallol, gallic acid, pentaploid tannins used as antiviral agents It is much better than antibacterial agents and the like, and 99% or more of these viruses can be eliminated. Usually, viruses have different structures depending on their types, so the behavior or resistance to chemical substances and chemicals is different for each type, but it is particularly useful for antiviral agents that use at least oyster extract. In that case, it has a feature that it is effective against a wide range of viruses regardless of the type of the virus.

  The textile product for antiviral hygiene of the present invention includes tissue, wet tissue, napkin, wet napkin, towel, wet towel, mask, auxiliary cloth in mask, sanitary clothing, antiviral filter, antiviral air conditioner filter, etc. It is provided for use and can be provided as a means for preventing infection and a method for preventing infection caused by non-enveloped viruses or enveloped viruses.

  The fiber product for antiviral hygiene of the present invention can efficiently disinfect and prevent infection of non-enveloped viruses or enveloped viruses in general households, food handling situations and medical institutions. It is expected that the occurrence of diseases caused by these viruses can be greatly suppressed.

(A)-(c) is a conceptual diagram showing the state of a virus when a sample is made to act on a norovirus. In (a), both the viral genome and the surface protein are normal, and the virus retains infectivity (no effect). (B) is a state in which the viral genome is retained, but the surface protein is destroyed and the infectivity is lost (effective). (C) is a state in which both the viral genome and surface proteins have been destroyed and have lost their infectivity (effective). It is a graph which shows the result of the norovirus inactivation test about the hand-washing foam agent and the hand lotion agent in Reference Example 1-2. It is a graph which shows the result of the stabilization test of the anti-norovirus activity by vitamin C about the hand-washing foam agent and the hand lotion agent in Example 1-3 [1]. It is a graph which shows the result of the stabilization test of the anti-norovirus activity by vitamin C in Example 1-3 [2]. It is a graph which shows the result of the synergistic effect test of the anti-norovirus activity by a citric acid in Example 1-4. FIG. 2-1 is a graph showing the measurement results of virus infectivity regarding Coxsackie virus in Reference Example 2-2. FIG. 2-2 is a fluorescent staining photograph of an adenovirus-infected cell with an anti-adenovirus antibody in Reference Example 2-3. FIG. 2-3 is a diagram showing the antiviral activity of various reagents against adenovirus in Reference Example 2-3. FIG. 2-4 shows an example of the plate after fixation and staining for the feline calicivirus test in Reference Example 2-4. FIG. 2-5 is a diagram showing the antiviral activity of various reagents against feline calicivirus in Reference Example 2-4. FIG. 2-6 is a photograph of fluorescent staining of rotavirus-infected cells with anti-rotavirus antibody in Reference Example 2-5. FIG. 3-1 shows the antiviral activity of various drugs against human influenza virus in Reference Example 3-1. FIG. 3-2 shows the antiviral activity of various drugs against the avian influenza virus in Reference Example 3-1. FIG. 3-3 shows the antiviral activity of various drugs against vesicular stomatitis virus in Reference Example 3-1. FIG. 3-4 shows the antiviral activity of various drugs against herpes simplex virus type 1 in Reference Example 3-1. FIG. 3-5 shows the antiviral activity of various drugs against Newcastle disease virus in Reference Example 3-1. FIG. 3-6 shows the antiviral activity of various drugs against the avian influenza virus in Reference Example 3-3. FIG. 3-7 shows the antiviral activity of various drugs against human influenza virus in Reference Example 3-4. FIG. 4-1 shows the antiviral activity of 5% scab-treated non-woven fabric and 0% scab-treated non-woven fabric (control) against avian influenza virus in Example 1. FIG. 4-2 shows the antiviral activity of 1%, 0.1%, 0.01%, 0.001%, 0.0001% wrinkle-treated nonwoven fabric and 0% wrinkle-treated nonwoven fabric (control) against avian influenza virus in Example 1.

− Non-enveloped virus −
The antiviral agent constituting the antiviral sanitary textile product of the present invention can be applied to a wide range of non-enveloped viruses.

  Non-enveloped viruses include Iridoviridae, Adenoviridae, Polyomaviridae, Papillomaviridae (with dsDNA); Circoviridae, Parvoviridae (with ssDNA); Reoviridae, Birnavirus Viruses such as Picornaviridae, Caliciviridae, Hepaviridae, Astroviridae, and Nodaviridae (with positive sense strand ssRNA) are known. Of non-enveloped viruses.

  More specifically, for example, the genus Norovirus (Norovirus); the genus Betanodavirus (such as Nervous Necrosis Virus (NNV)); the genus Aquavirnavirus (such as infectious pancreatic necrosis virus); Aquareovirus genus; Lanavirus genus (RSBI: Red Sea Bream Iridovirus, etc.) or Parvoviridae (Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV), etc.); Non-enveloped viruses known as fish disease viruses and the like belonging to each of the Toroviridae (Masstroberry disease virus, Taura syndrome virus, etc.).

  In addition, enterovirus genus (coxsackie virus, enterovirus, poliovirus, echovirus, etc.); hepatovirus genus (hepatitis A virus etc.); rhinovirus genus (rhinovirus etc.); sapovirus genus (sapovirus etc.); Human astrovirus etc.); papillomavirus genus (papillomavirus etc.); polyomavirus genus (polyomavirus etc.); mast adenovirus genus (human adenovirus etc.); rotavirus genus (rotavirus etc.), Non-enveloped viruses known as human pathogenic viruses and other non-enveloped viruses such as feline calicivirus belonging to the genus Besivirus can also be targeted.

− Envelope virus −
The antiviral agent constituting the antiviral sanitary textile product of the present invention can also be applied to a wide range of enveloped viruses. For example, the present invention can be applied to the following envelope viruses known as pathogenic viruses of humans, livestock (mammals and birds) or fish.

Flaviviridae: Flaviviridae [Japanese encephalitis virus (+ sense single-stranded RNA), West Nile virus (+ sense single-stranded RNA), etc.], Hepacivirus [hepatitis C virus (+ sense single-stranded) RNA) etc.];
Coronaviridae: Coronavirus genus [SARS virus (+ sense single-stranded RNA) etc.];
Rhabdoviridae: Rissavirus genus [rabies virus (-sense single-stranded RNA), etc.], vesiculovirus genus [vesicular stomatitis virus (-sense single-stranded RNA), etc.];
Filoviridae: Marburg virus genus [Marburg virus (-sense single-stranded RNA) etc.], Ebola virus genus [Ebola virus (-sense single-stranded RNA) etc.];
Paramyxovirae (Paramyxovidae)
Paramyxovirinae: Rubravirus [mumps virus (-sense single-stranded RNA), etc.], Morbillivirus [measles virus (-sense single-stranded RNA), etc.], Avuravirus [Newcastle disease virus ( -Sense single-stranded RNA) etc.];
Pneumovirus subfamily: Pneumovirus genus [RS (respiratory syncytial) virus (-sense single-stranded RNA) etc.], metapneumovirus genus [metapneumovirus (-sense single-stranded RNA) etc.];
Orthomyxoviridae: influenza A virus genus [influenza A virus (-sense 8-segment RNA), etc.];
Bunyaviridae: Hantavirus genus [Hantavirus (+/- sense triple-stranded RNA) etc.];
Arenaviridae: Arenavirus genus [Lassa virus (+/- sense double-stranded RNA) etc.];
Retroviridae: Delta retrovirus genus [human T lymphocyte-tropic virus (+ sense single-stranded RNA) etc.], lentivirus [human immunodeficiency virus (+ sense single-stranded RNA) etc.];
Herpesviridae (Herpesviridae)
Alphavirus subfamily: simple virus genus [herpes simplex virus type 1 (double-stranded DNA) etc.];
Ictalurivirus [Ictalurid herpesvirus 1, double-stranded DNA virus, sphere, etc.];
(Unknown unclassified) Koi herpesvirus;
Rhabdoviridae: Novirhabdoviridae [Viral hemorrhagic septicemia virus (VHSV), single-stranded RNA virus, cannonball type, etc.];
Reoviridae: (genus / species unknown) goldfish capsized virus;
Nimaviridae: Whispovirus [White spot syndrome virus (double-stranded DNA virus, oval), etc.];
Roniviridae: Okavirus [Yellow head virus (single-stranded DNA virus, spheroid), etc.];
Baculoviridae: Nucleopolyhedrovirus [monodon baculovirus (Penaeus monodon NPV, double-stranded DNA virus, atypical), etc.].

− Antiviral agent −
-Applicability of antiviral agent and target virus In the present invention, the active ingredient of the antiviral agent impregnated in the antiviral sanitary textile product is oyster extract, catechin, depending on the target non-enveloped virus or envelope virus Appropriate ones can be selected from the following classes: wattle tannin, pentagalloyl glucose, coffee tannin, gallic acid alkyl ester (excluding those in oyster extract), pyrogallol, gallic acid, pentaploid tannin and the like. The antiviral agent used in the present invention has applicability to non-enveloped viruses or enveloped viruses as shown below, for example.
[A] As a non-enveloped virus or an enveloped virus, a genus of mast adenovirus, genus besivirus, rotavirus, genus betanodavirus, genus aquavirnavirus, genus ranavirus, genus influenza A virus, genus vesiculovirus, When targeting viruses other than the simple virus genus, the abular virus genus and the nobirabrado genus, the oyster extract is used as the active ingredient.
[B] When targeting a virus of the genus mastadenovirus as the non-enveloped virus, at least one selected from the group consisting of oyster extract, catechins and wattle tannin is used as an active ingredient.
[C] When targeting a virus of the genus Besivirus as a non-enveloped virus, at least selected from the group consisting of oyster extract, catechins, gallic acid alkyl esters (excluding those in oyster extract) and pyrogallol One type is an active ingredient.
[D] When targeting a virus belonging to the genus Rotavirus as a non-enveloped virus, oyster extract, catechins, pentagalloyl glucose, coffee tannin, gallic acid alkyl ester (excluding those in oyster extract) and pyrogallol The active ingredient is at least one selected from the group consisting of
[E] A group consisting of oyster extract, wattle tannin, pentagalloyl glucose and catechins when a virus of the genus Betanodavirus, Aquavirnavirus and / or Ranavirus is targeted as a non-enveloped virus At least one selected from the above is used as an active ingredient.
[F] When the envelope virus is a virus belonging to the genus Influenza A or simple virus, at least one selected from the group consisting of oyster extract, catechins, wattle tannin, pentagalloyl glucose, and pyrogallol Is the active ingredient.
[G] When the envelope virus is a virus belonging to the genus Becyclovirus, at least one selected from the group consisting of oyster extract, catechins, wattle tannin, and pyrogallol is used as an active ingredient.
[H] When the envelope virus is a virus belonging to the genus Aburavirus, the active ingredient is at least one selected from the group consisting of oyster extract, catechins, wattle tannin, and pentagalloyl glucose.
[I] When the envelope virus is a virus belonging to the genus Nobilabdovirus, it was selected from the group consisting of oyster extract, catechins, wattle tannins, pentagalloyl glucose, coffee tannins, pyrogallol, gallic acid and pentaploid tannins At least one kind is an active ingredient.

-Oyster extract In the present invention, as an active ingredient of an antiviral agent against a wide range of non-enveloped viruses or enveloped viruses, an extract of the plant of the genus Oyster that contains tannin, that is, an oyster extract (sometimes called oyster shibutannin) .). In addition, this oyster extract is not only a purified product derived from a plant belonging to the genus Oyster which contains tannin, but also an aspect in which it is mixed with a solvent used to extract this, an alcohol used for alcohol treatment, or the like. May be used.

  The raw material of the oyster extract is not particularly limited, but it is efficient to use immature fruits of astringents (for example, oyster oysters, varieties such as flat seedless) that are rich in oyster tannins (especially condensed tannins) And economical. Moreover, as long as the oyster extract which consists of a similar component is obtained, it is good also considering a site | parts other than fruits, such as a leaf and a bark of a plant of the genus Oyster.

  A method for preparing an oyster extract from such a raw material is not particularly limited, but generally, a method for recovering squeezed juice by pulverizing and compressing an astringent from which cocoons have been removed, or an appropriate size. For example, a method in which the mixture is cut into a liquid and then liquefied by a mixer, and the supernatant is collected by a centrifuge, or a method in which the extract is collected by extraction with water or an aqueous solvent is used.

-Heating, alcohol and other treatments As the oyster extract of the present invention, the juice and extract of the fruits of the genus Oyster can be used as they are, but they are heated or treated with alcohol. Therefore, it is desirable to use a product obtained by inactivating the enzyme derived from the plant belonging to the genus Genus contained therein (hereinafter also referred to as “processed oyster extract”). The antiviral properties of the oyster extract are further increased by heating or alcohol treatment, and at the same time, the measurement for verifying the antiviral properties can be prevented from being inhibited. Such a processed product of oyster extract can be prepared relatively easily and has the advantage that color and odor are hardly a problem when used as a component of the composition, and it takes time for fermentation. It is easier to use industrially than amber (see below), which is difficult to use in terms of color and odor. Further, even when stored for a long time by heating or alcohol treatment as described above, no discoloration or precipitation occurs.

  The temperature and time conditions of the heat treatment may be any conditions that can inactivate enzymes derived from plants belonging to the genus Oyster that are contained in juices, etc., and are generally used to deactivate plant enzymes. Conditions can be adopted. That is, the temperature is usually 60 to 130 ° C., and the time is usually 5 seconds to 30 minutes. For example, the heat treatment is performed at 120 ° C. to 130 ° C. for 5 to 10 seconds, or at about 85 ° C. for 5 to 15 minutes. Just do it. Moreover, the aspect of a heat processing process is not specifically limited, For example, the heating in the process which sterilizes juice, the heating in the process which pulverizes, or before the fermentation start for manufacturing a persimmon astringent (details are mentioned later) The treatment can be performed by heating or the like.

  On the other hand, the alcohol treatment can be usually performed using 30 to 100%, preferably 50 to 100% alcohol such as ethanol. For example, it is preferable to treat the oyster juice so as to add approximately the same amount of 95 v / v% ethanol, and then store it in a sealed light-shielding container (usually a light brown solution). Also, the aspect of the alcohol treatment step is not particularly limited, and for example, the extraction step using an alcohol solvent such as ethanol corresponds to the alcohol treatment step, and typically the antiviral properties of the present invention. As one step for producing an alcohol preparation as a composition, alcohol can be added to the oyster extract that has been subjected to the above heat treatment, if necessary, and mixed. Such alcohol treatment also has an effect of sterilizing miscellaneous bacteria contained in the oyster extract itself.

  The oyster extract of the present invention is solidified by concentration, drying, or freeze-drying as necessary, in addition to the above-mentioned treatment, or in addition to the above-mentioned treatment, as long as the effects of the invention are not impaired. In addition, pulverization (freeze-dried powder is usually slightly yellow) or purification using an ion exchange resin or the like may be performed. The oyster extract contains a large amount of polyphenol, and if it remains in a liquid state, it tends to undergo coloration and other alterations. Therefore, it is desirable that the oyster extract be stored frozen in a solid state using a freeze-drying method. These operations are preferably performed under mild conditions such that oyster tannin and other components contained in the oyster extract are not decomposed.

  In addition, the oyster extract in the present invention is a liquid obtained by squeezing immature astringency and then fermenting and aging for a long period (about 1 to 3 years), and has a solid content of about several percent (oyster tannin) ) And organic acid produced by fermentation or the like may be used. Conventionally, this persimmon has been used as a folk medicine or paint, and is generally marketed as a product such as “Tsubaki Shibu” (Takeyama Shibu Production Center). Alternatively, a fermentation product obtained by adding oyster-derived yeast culture solution to oyster juice and fermenting at 20 to 25 ° C. for 1 to 3 months can be used (usually a reddish brown solution), and then a sealed container It is preferable to store within.

  It should be noted that the “List of Existing Additives Listed Items” in accordance with the Japanese Food Sanitation Law includes “柿 tannin” (product name / alias = 柿 shibu, 柿 extract. Basic, manufacturing method, essence = oyster oyster Diospyros kaki THUNB. The additive described as squeezed or obtained by extraction with water or ethanol. The main components are tannin and tannic acid.) The additive described as oyster extract in the present invention is used. You can also

-Oyster Tannins Plants of the genus Diospyros, especially their fruits, are generically called so-called tannins, which have certain properties, such as astringency and binding to metal ions, that make them feel astringent. Rich in compounds. Such oyster tannins often contain “oyster condensed tannin” represented by the following estimated structural formula (I), which is formed by binding catechin, gallocatechin and gallate esters thereof as a main component. It is characteristic that it is. For example, among the plants belonging to the genus Oyster, oyster-condensed tannins contained in the fruit of Diospyros kaki, native to China and widely cultivated worldwide, include catechin, catechin gallate, gallocatechin and gallocatechin. It is a polymer compound in which gallate is condensed with a carbon-carbon bond at a ratio of about 1: 1: 2: 2 (Matsuo & Itoo (1981): see Non-Patent Document 1 above).

  Further, oyster tannins may contain other tannic compounds such as catechins and hydrolyzable tannins in addition to the above “oyster condensed tannins”.

  Hydrolyzed tannin is an ester of an alcohol (such as glucose) and a carboxylic acid (such as gallic acid) or an oligomer thereof, and its molecular weight is reduced by hydrolysis, whereas oyster condensed tannin is hydrolyzed. It can be distinguished from hydrolyzed tannins because it does not reduce the molecular weight (the carbon-carbon bond of the basic skeleton of the polymer is not hydrolyzed). For example, it is described in Matsuo & Itoo (1981b) (Non-patent Document 2). It is also possible to purify and quantify by a method that has been developed.

  For antiviral expression against non-enveloped virus or enveloped virus in the present invention, it is presumed that oyster extract preferably contains at least oyster condensed tannin, but other oyster extract The possibility of the contribution of this component is not excluded. The oyster extract obtained by the preparation method as described later using oyster fruit usually contains oyster condensed tannin, and the antiviral agent of the present invention uses such an oyster extract. It is presumed that it is preferable to use it as a raw material.

・ Catechins Non-enveloped viruses such as betanodavirus, aquavirnavirus, ranavirus, mastadenovirus, vesivirus, rotavirus, etc., or influenza A virus genus, becyclovirus When targeting envelope viruses such as the genus, simple virus genus, abula virus genus, and nobirabrado genus, catechins can also be used as active ingredients.

  Catechins are shrubs of Indian leguminous acacia genus, which are contained in plants such as catechu (also called gan beer; scientific name: Acacia catechu) and tea tree (tea tree, scientific name: Camellia sinensis). 7,3 ′, 4′-pentahydroxyflavan (catechin in the narrow sense), 3,5,7,3 ′, 4 ′, 5′-hexahydroxyflavan (gallocatechin) and their 3-galloyl derivatives, These stereoisomers are also included. For example, (+)-catechin separated from catechu has the following structural formula (2).

  In addition, catechins (sometimes referred to as “green tea tannins”) contained in tea tree are epicatechin [epicatechin, structural formula (3)] and epigallocatechin of its hydroxy form [epigallocatechin, structural formula (4) )], And their gallic acid esters epicatechin gallate [epicatechin gallate, structural formula (5)] and epigallocatechin gallate [epigallocatechin gallate, structural formula (6)] as main components, these compounds are heated. It may be isomerized by treatment.

  Catechins are also contained in other plants. In the present invention, these compounds may be isolated and used from a plant containing catechins, or an extract of a plant containing catechins may be used. Moreover, any one catechin may be used alone, or two or more catechins may be used in combination. It is most convenient to use catechins contained in chanoki.

・ Watrutannin Antiviral agent is a non-enveloped virus such as Betanodavirus, Aquavirnavirus, Ranavirus, Mastadenovirus, Rotavirus, or influenza A virus, Becyclovirus, simple In the case of targeting envelope viruses such as the genus of virus, genus abyravirus, genus nobirabuvirus, watrutannin can also be used as an active ingredient.

  Watrutannin is a tannin extracted from the bark, leaves, and cocoons of plants of the genus Acacia. For example, Acacia pycnantha (Golden Wattle), Acacia decurrens (Tan Wattle), Acacia dealbata (Silver Wattle, Mimosawaturu) And Acacia mearnsii (Black Wattle).

・ Pentagalloyl glucose Antiviral agent is a non-enveloped virus such as Betanodavirus, Aquavirnavirus, Ranavirus, Rotavirus, etc., or influenza A virus, simple virus, Avuravirus, When targeting envelope viruses such as the genus Billabudovirus, pentagalloylglucose can also be used as an active ingredient.

  Pentagalloyl glucose is a compound in which gallic acid is ester-bonded to hydroxyl groups (1, 2, 3, 4 and 6 positions) of glucose, and can be obtained, for example, by decomposing and purifying pentaploid tannin as a raw material.

-Coffee tannin When the antiviral agent targets a non-enveloped virus such as rotavirus genus or an envelope virus such as nobirabrado genus, coffee tannin can also be used as an active ingredient.

  Coffee tannins are tannins (or tannin-like substances) extracted from coffee beans, etc., and mainly caffeic acid esterified with chlorogenic acids (hydroxyl groups of quinic acid (any one or more of the 3, 4 or 5 positions)). Compound).

・ Algal ester of gallic acid When antiviral agent targets non-enveloped viruses such as Besivirus genus, Rotavirus genus, gallic acid alkyl ester (gallic acid (3,4,5-trihydroxybenzenecarboxylic acid)) Can be used (the epicatechin gallate, epigallocatechin gallate, etc. contained in the pentagalloyl glucose and catechins mentioned above are not included). Except when using oyster extract to do).

  Examples of the gallic acid alkyl ester include ethyl gallate, n-propyl gallate, n-butyl gallate, n-pentyl gallate, n-octyl gallate, n-nonyl gallate, n-decyl gallate, isobutyl gallate, isoamyl gallate, and the like. -Butyl gallate is preferred. In addition, gallic acid alkyl ester (n-propyl gallate or the like) is also used as an antioxidant (food additive).

・ Pyrogallol Effective when antiviral agents target non-enveloped viruses such as Vesiviruses, Rotaviruses, etc., or envelope viruses such as influenza A, Becycloviruses, Simple Viruses, and Nobirabridoviruses Pyrogallol (1,2,3-trihydroxybenzene) can also be used as a component.

-Gallic acid When an antiviral agent targets envelope viruses such as Nobilabdovirus, gallic acid can be used as an active ingredient.

-Ploidy tannin When an antiviral agent targets an envelope virus such as Nobilabdovirus genus, a pentaploid tannin derived from a quintuplet (nulde insect gallbladder) can be used as an active ingredient.

− Antiviral composition −
The textile product for antiviral hygiene of the present invention is produced by impregnating at least one fibrous base material selected from the group consisting of paper, woven fabric and non-woven fabric with an antiviral composition. Is preferred. This antiviral composition is preferably appropriately selected according to the use of tissue, wet tissue, napkin, wet napkin, towel, wet towel, mask, sanitary clothing, antiviral filter, and antiviral air conditioner filter. .

  The antiviral composition includes an antiviral agent [A] as an active ingredient against a non-enveloped virus or an enveloped virus, an alcohol [B], a surfactant [C], an antibacterial agent [D], a moisturizing agent [E], Alternatively, it is a composition containing any one or more components of cosmetic fats and oils [F], and further containing citric acid and / or a salt thereof and vitamin C as necessary. Although the aspect is not specifically limited, The following are mentioned typically.

[Composition I] Alcohol preparation composition containing at least an antiviral agent [A] and an alcohol [B] In accordance with the purpose of the antiviral sanitary textile product, water or a surfactant [C] is further added. be able to. This composition is applied to antiviral tissues, wet tissues, napkins, wet napkins, towels, wet towels and the like.

[Composition II] Cleaning composition containing at least antiviral agent [A] and surfactant [C] This composition is antiviral and is intended for cleaning, wet tissue, napkin Applicable to wet napkins, towels, wet towels, etc.

[Composition III] Disinfecting composition containing at least antiviral agent [A] and antibacterial agent [D] This composition comprises antiviral / antibacterial tissue, wet tissue, napkin, wet napkin, towel, Applicable to wet towel, mask, auxiliary cloth in mask, sanitary clothing, antiviral filter, antiviral air conditioner filter, etc.

[Composition IV] A moisturizing composition containing at least an antiviral agent [A] and a humectant [E] This composition comprises an antiviral tissue, a wet tissue, a napkin, a wet napkin, a towel, a wet towel, It is applied to masks, auxiliary cloths in masks, sanitary clothing, antiviral filters, antiviral air conditioner filters, etc.

  The alcohol preparation composition of [Composition I] is a non-enveloped virus adhering to food, tableware, cooking utensils, fish and other rearing equipment, rearing water tanks, workers 'hands, or equipment handling patients' filth. An embodiment of the composition used to inactivate enveloped viruses. The antiviral sanitary textile product impregnated with this composition is used to inactivate non-enveloped or enveloped viruses attached by wiping the object. The antiviral sanitary textile product impregnated with the composition is applied to a wiping material such as a tissue, a wet tissue, a napkin, a wet napkin, a towel, and a wet towel.

  The cleaning composition of [Composition II] removes dirt such as food, tableware, cooking utensils, fish and other breeding equipment, fish tanks, workers' hands and clothes, and does not contain non-enveloped or enveloped viruses. It is a composition of the aspect which can be activated. The textile product for antiviral hygiene impregnated with this composition is used as a wiping material for wiping and washing the above object and inactivating the attached non-enveloped virus or enveloped virus by wiping. Is done. The antiviral sanitary textile product impregnated with the composition is applied to a wiping material such as a tissue, a wet tissue, a napkin, a wet napkin, a towel, and a wet towel.

  The disinfecting composition of [Composition III] is a non-enveloped virus attached to food, tableware, cooking utensils, fish and other rearing equipment and aquariums, workers 'hands, or equipment handling patients' filth. It is a composition of the aspect used in order to inactivate an envelope virus and to also provide a bactericidal action. The antiviral sanitary textile product impregnated with this composition inactivates non-enveloped viruses or enveloped viruses by wiping the object, and is also used for sterilization. The anti-viral sanitary textile product impregnated with this composition is applied to wiping materials such as anti-viral and antibacterial tissues, wet tissues, napkins, wet napkins, towels, wet towels and the like. In addition, the anti-viral sanitary textile product impregnated with this composition comprises a mask for preventing infection with bacteria at the same time as preventing infection with non-enveloped virus or envelope virus, an auxiliary cloth in the mask, and sanitary clothing. It is also applied to applications such as antiviral filters and antiviral air conditioner filters.

  The moisturizing composition of [Composition IV] is a non-enveloped virus adhering to food, tableware, cooking utensils, fish and other breeding equipment, fish tanks, workers' hands, or equipment handling patient's filth. An embodiment of the composition used to inactivate enveloped viruses. The antiviral sanitary textile product impregnated with this composition is used to inactivate non-enveloped or enveloped viruses attached by wiping the object. The anti-viral sanitary textile product impregnated with this composition is applied to wiping materials such as anti-viral tissue, wet tissue, napkin, wet napkin, towel, wet towel and the like. Anti-viral sanitary textile products impregnated with this composition are masks for preventing infection with non-enveloped viruses or envelope viruses, auxiliary cloths in masks, anti-viral filters, anti-viral air conditioner filters, etc. It is also applied to other uses.

  In addition, in the antiviral composition of the above [Composition I] to [Composition IV], as a matter of course, two or more kinds of alcohol, surfactant, antibacterial agent or moisturizing agent may be used in combination. For example, for an alcohol preparation composition, it is also preferable to further add a surfactant such as a fatty acid ester in order to further improve antibacterial properties. In addition, the cleaning composition can take an aspect in which an antibacterial agent or alcohol is blended in addition to the surfactant, and the moisturizing composition keeps the hand skin clean with components for protecting the hand skin. The antibacterial agent and alcohol can be used.

  Furthermore, in addition to the components specifically described in the present specification, the antiviral compositions of [Composition I] to [Composition IV] impart desired performance, and Various ingredients to enhance quality, such as cosmetic fats and oils such as beeswax, thickeners (xanthan gum, locust bean gum, sodium polyacrylate, etc.), antioxidants, fragrances, pigments, skin roughening agents, anti-inflammatory agents, A fixing agent for the fibrous base material can be appropriately blended.

-Content of antiviral agent The content of the antiviral agent in the antiviral composition of [Composition I] to [Composition IV] is within a range where antiviral properties against non-enveloped or enveloped viruses are expressed. The active ingredient such as oyster extract in the antiviral agent or its processed product is an antiviral composition. The amount is usually 0.01 to 10% by weight, preferably 0.01 to 5% by weight, more preferably 0.1 to 2% by weight, and still more preferably 1 to 2% by weight, based on the whole product. That's fine.

  The content of the above-mentioned antiviral agent is based on the “solid content”. When a liquid material such as an extract of persimmon fruit is used as a production raw material, the solid content (dried in the liquid material) Alternatively, the blending amount of the liquid material may be adjusted so that the weight of the powder obtained by freeze-drying is within the above range. The squeezed coconut juice usually contains about 5 to 10% solid content.

Organic acid and / or salt thereof The antiviral composition impregnated in the fiber product of the present invention includes oyster extract (oyster tannin), catechins, wattle tannin, pentagalloyl as an active ingredient of an antiviral agent. In addition to glucose, coffee tannins, alkyl esters of gallic acid (excluding those in oyster extracts), pyrogallol, gallic acid, pentyl tannins, etc., organic acids other than those contained in oyster extracts and / or their salts (In the present specification, “organic acid and / or salt thereof” to be blended with the antiviral composition is excluded from what is in the oyster extract, even if not specifically stated. To do).

  In particular, when the antiviral agent targets a non-enveloped virus such as Besivirus genus or Rotavirus genus, or an envelope virus such as influenza A genus, Becyclovirus genus, simple virus genus, or Nobirabud virus genus, organic Acids and / or their salts can be active ingredients against these viruses. In addition, depending on the type of non-enveloped virus or envelope virus, the organic acid and / or salt thereof may not exhibit antiviral properties by itself, but when used in combination with an active ingredient such as the above-mentioned oyster extract, The effect can be further enhanced.

  In addition, organic acids and / or their salts are substances with excellent antibacterial properties against bacteria and are recognized as food additives. In addition to increasing the solubility of tannin, coloring when tannin comes into contact with iron Also acts as a chelating agent to prevent From such a viewpoint, it is also preferable to add an organic acid and / or a salt thereof to the antiviral composition.

  The organic acid and / or salt thereof is preferably an organic acid having 2 to 10 carbon atoms and / or a salt thereof, more preferably an organic acid containing a hydroxyl group having 2 to 10 carbon atoms and / or a salt thereof. More specifically, at least one organic acid selected from the group consisting of lactic acid, malic acid, citric acid, tartaric acid, salicylic acid, succinic acid, fumaric acid and itaconic acid and / or a salt thereof is preferable. Or its salt is preferable. Moreover, as a salt of an organic acid, the sodium salt of the said organic acid, potassium salt, etc. are preferable.

  When an organic acid and / or a salt thereof is added to the antiviral composition of the present invention as an active ingredient for a specific envelope virus and / or for other purposes as described above, the organic acid and / or its The proportion of the salt is preferably 0.05 to 5.0% by weight, more preferably 0.1 to 2.0% by weight, based on the whole antiviral composition (including the solvent and the like). Alternatively, the pH of the antiviral composition is usually 6.0 to 2.0, preferably 5.0 to 2.0, and more preferably 4.8 to 2.0. And more preferably in an amount of 4.0 to 2.0. The pH of the aqueous solution may be measured using a general pH meter (for example, a pH meter manufactured by Beckman Coulter, Inc.).

Vitamin Cs In addition to the above-mentioned antiviral agents, vitamin Cs known as antioxidants added to foods and the like are blended in the antiviral composition impregnated in the fiber product of the present invention. Is preferred. Here, “vitamin Cs” is a general term including DL-ascorbic acid, ascorbic acid ester (such as palmitic acid ester) and the like in addition to L-ascorbic acid generally called vitamin C. Addition of vitamin C suppresses oxidation of oyster extracts (especially oyster tannins), and the effect against non-enveloped viruses can be demonstrated stably and continuously. It can also be suppressed.

  The amount of vitamin C added is preferably 0.01 to 5.0% by weight, preferably 0.05 to 2.0% by weight, based on the whole antiviral composition (including solvent and the like). Is more preferable.

-Alcohol In addition to the above-mentioned antiviral agent, an alcohol may be added to the antiviral composition impregnated in the textile product of the present invention in accordance with the purpose of the antiviral sanitary textile product. As the alcohol, those similar to general alcohol preparations can be used, but ethanol and / or propanol which have excellent antibacterial properties against bacteria and are recognized as food additives are preferable. The concentration of these alcohols may be about the same as that of a general alcohol preparation and can be adjusted in consideration of antibacterial properties, but is preferably about 10 to 80% with respect to the whole alcohol preparation. Alcohol may be used as a solvent in a composition other than an alcohol preparation, and may be blended in cosmetics or the like as a component that provides astringency and antiseptic properties to the skin.

Surfactant In addition to the antiviral agent described above, a surfactant may be added to the antiviral composition impregnated in the textile product of the present invention in accordance with the purpose of the antiviral sanitary textile product. . Surfactants include cationic, anionic, zwitterionic and nonionic surfactants. In view of chemical properties such as oyster condensed tannin (polyphenol), the present invention includes anionic surfactants and It is preferable to use a nonionic surfactant.

  Examples of the anionic surfactant include soap (alkali salt of higher fatty acid), monoalkyl sulfate, alkyl polyoxyethylene sulfate, alkyl benzene sulfonate, monoalkyl phosphate, and the like.

  Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, fatty acid partial ester of polyhydric alcohol (glycerin, sugar alcohol, etc.), fatty acid diethanolamide and the like.

  Among these surfactants, those recognized as food additives such as glycerin fatty acid partial ester, sorbitan fatty acid partial ester, and sucrose fatty acid partial ester do not pose a problem even if they adhere to food, tableware or cooking utensils. Therefore, it is a preferable surfactant in the present invention.

  The surfactants described above also have an action of destroying bacterial cell membranes and viral envelopes. For example, partial esters of glycerin with fatty acids having 6 to 18 carbon atoms (capric acid having 10 carbon atoms, etc.) Because of its excellent antiviral properties against enveloped viruses and antibacterial properties against Escherichia coli and Staphylococcus aureus, it is also suitable to incorporate these surfactants as active ingredients in the aforementioned antiviral agents or in compositions such as alcohol preparations. It is. The surfactant is also used as a component for mixing the oil phase and the aqueous phase in creams and emulsions.

Antibacterial agent In addition to the antiviral agent described above, the antiviral composition impregnated into the textile product of the present invention includes organic acids such as ethanol and citric acid described above depending on the purpose of the antiviral sanitary textile product. And antibacterial agents (including substances called bactericides and disinfectants) other than salts thereof (including antibacterial agents and disinfectants) (in this specification, “antibacterial agents” to be added to antiviral compositions are particularly described. Even if not, remove ethanol, organic acids and their salts). The antibacterial agent is not particularly limited, but known antibiotics and synthetic antibacterial agents, for example, Escherichia coli, Staphylococcus aureus, MRSA, Salmonella, Vibrio parahaemolyticus, green, which cause infection during food processing or hospital infection. Those effective against Pseudomonas aeruginosa are preferred. Among them, synthetic antibacterial agents such as isopropylmethylphenol, butyl parahydroxybenzoate, and triclosan are preferable antibacterial agents in the present invention in terms of excellent antibacterial action and compatibility with oyster extract. In addition, the substance used as an antibacterial agent as described above may be blended in the ethanol preparation described above, and may be used as a preservative in cosmetics and the like.

-Moisturizer In the antiviral composition impregnated in the textile product of the present invention, in addition to the antiviral agent described above, a humectant can be blended according to the purpose of the textile product for antiviral hygiene. Moisturizing agents (wetting agents) that can be used in the present invention are the same as those used in cosmetics such as general lotions, emulsions, and creams. For example, glycerin, propylene glycol, sorbitol, polyethylene glycol, hyaluron Acid, sodium chondroitin sulfate, ceramide, aloe extract and the like can be mentioned. Among these humectants, those recognized as foods or food additives such as aloe extract are preferred humectants in the present invention in that they do not pose a problem even if they adhere to foods, tableware, and cooking utensils.

・ Cosmetic oils and fats In addition to the antiviral agents described above, the antiviral composition impregnated in the fiber product of the present invention should contain cosmetic oils and fats according to the purpose of the antiviral hygiene fiber product. Can do. Cosmetic fats and oils are components for forming a film on the skin surface to play a role in protecting the skin, providing flexibility and lubricity, and imparting an appropriate feeling to use in cosmetics. In the present invention, cosmetic oils and fats similar to those used in general cosmetics such as emulsions and creams can be used, and examples thereof include the following.

・ Fats and oils (esters of higher fatty acids and glycerin) ... vegetable oils, animal fats and oils or hydrogenated products thereof (partially hydrogenated rapeseed oil, etc.), synthetic triglycerides (tri (capryl / capric acid) glyceryl, etc.), etc .;
・ Wax (esters of higher fatty acids and higher alcohols that are solid at room temperature): vegetable wax, animal wax (eg, beeswax, lanolin, etc.);
・ Hydrocarbons: mineral hydrocarbons (liquid paraffin, petrolatum, paraffin, etc.), animal hydrocarbons (squalane, etc.);
・ Higher fatty acids: Lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, etc .;
・ Higher alcohol: cetanol, stearyl alcohol, lanolin alcohol, etc .;
Esters (esters of fatty acids and alcohols other than waxes): myristyl myristate, propylene glycol dioleate, cetyl lactate, etc.

  Among these cosmetic oils and fats, those recognized as foods or food additives such as beeswax are preferable cosmetic oils and fats in the present invention in that they do not pose a problem even if they adhere to foods, tableware, and cooking utensils. .

-Manufacturing method of antiviral composition The manufacturing method of the antiviral composition impregnated into the textile product of the present invention is blended with an antiviral agent (such as oyster extract) as one of the manufacturing raw materials, and if necessary In addition to blending organic acids and / or their salts and vitamin C, and adjusting production processes and conditions accordingly, conventional alcohol preparations, detergents, disinfectants, cosmetics (lotions) , Emulsion, cream) and the like. That is, in addition to the conventional (or fine-tuned if necessary) production raw materials of these conventional products, antiviral agents are blended, and the same as the conventional products (or fine-tuned as necessary) by the production process, The antiviral composition of the present invention can be produced.

  In addition, when an organic acid and / or a salt thereof is added to the antiviral agent or antiviral composition of the present invention (particularly, non-enveloped viruses such as Vesivirus genus, Rotavirus genus, influenza A virus genus, vesiculovirus) As described above, the pH of the aqueous solution is preferably 5.0 to 2.0, more preferably 4.8 in the case of envelope viruses such as virus genus, simple virus genus, and nobirlabd virus genus. It is preferable to add an organic acid and / or a salt thereof in an amount of -2.0, more preferably 4.0-2.0.

-Antiviral sanitary textile products-
-Fibrous base material The fibrous base material which comprises the textile product for antiviral hygiene of this invention is at least 1 type of fibrous base material chosen from the group which consists of paper, a woven fabric, and a nonwoven fabric. As a wiping material such as a tissue, a wet tissue, a napkin, a wet napkin, a towel, and a wet towel, any of paper, woven fabric, and non-woven fabric can be used. Also, as a fibrous base material for masks for preventing infection with non-enveloped viruses or enveloped viruses, auxiliary cloths in masks, sanitary clothing, antiviral filters, antiviral air conditioner filters, paper, woven fabrics or Any non-woven fabric can be used.

-Manufacturing method of antiviral sanitary textile product When manufacturing the antiviral sanitary textile product of the present invention, on at least one surface of a fibrous base material (also referred to as web) such as paper, woven fabric, and non-woven fabric. Apply an antiviral composition as described above. The antiviral composition may be applied to either one or both sides of the fibrous substrate, but is preferably applied to both sides of the substrate.

  For example, antiviral sanitary textile products in the form of wet tissues, wet napkins, wet towels, wet masks, wet filters, etc. (wet type) are coated with an aqueous solution of an antiviral composition on one or both sides of the substrate. , Spraying, impregnation and the like.

  On the other hand, anti-viral sanitary textile products in the form of dry tissues, dry napkins, dry towels, dry sanitary garments, dry filters, etc. (dry type) can also be used with an aqueous solution of the anti-viral composition on one side of the substrate or It can be produced by coating, spray printing, impregnation and the like on both sides. The dry type antiviral sanitary textile product is preferably manufactured by applying the antiviral composition after drying until the moisture content of the substrate reaches a predetermined value according to the application (that is, “dry web”). The “addition method” can be manufactured by preparing the wet type described above and drying it so that the moisture content becomes a predetermined value. In this case, the antiviral composition is applied in the range where the wet type is dried. Such relatively low levels of antiviral compositions impart softness and lotion-like feel to the substrate, and do not significantly affect absorbency, hydration, and particularly strength.

  The amount of the antiviral composition to be applied to the antiviral sanitary textile is not particularly limited. For example, in the range of 2 to 80% by weight with respect to the antiviral sanitary textile, wet type or dry What is necessary is just to adjust suitably according to a type. In addition, the application amount of the antiviral agent contained in the antiviral composition, in particular, an active ingredient (in terms of solid content) such as oyster extract in the antiviral agent or a processed product thereof is also the application amount of the antiviral composition. Or an antiviral agent contained in the composition, in particular, adjusting the amount of active ingredients such as oyster extract in the antiviral agent or a processed product thereof, depending on the mode of the textile product for antiviral hygiene It can be adjusted and is not particularly limited.

  In order to evenly distribute the solution or melt of the antiviral composition on the substrate surface, various known methods can be used. For example, dipping, coating, spraying, printing (for example, flexographic printing method) ), Coating (eg, gravure coating), extrusion, or a combination thereof. Exemplifying the application method, the antiviral composition is sprayed onto a rotating surface (for example, a calender roll), and then the composition attached to the rotating surface is transferred to the surface of the substrate. When applying the antiviral composition of the present invention to a substrate, gravure coating and extrusion coating methods are preferred.

  The antiviral composition is heated to a temperature in the range of 35-100 ° C, preferably 40-90 ° C, before being applied to the substrate. Once the antiviral composition is applied to the substrate, it is typically cooled and solidified to form a solidified coating or film on the substrate surface.

  The antiviral composition is usually applied uniformly to the surface of the substrate, but may be applied unevenly. “Homogeneous” means that the amount, distribution pattern, etc. of the antiviral composition varies across the surface of the substrate. For example, certain portions of the substrate can have a greater or lesser amount of an antiviral composition (including a surface that does not include any antiviral composition).

  The antiviral sanitary textile product of the present invention is usually produced by processing a substrate to which the antiviral composition is applied as described above for each use, but the fibrous base material has a desired shape. It is also possible to manufacture the antiviral composition by applying it on one or both sides by a dipping, coating or spraying method.

-Antiviral sanitary textile products The antiviral sanitary textile products of the present invention are materials for wiping such as tissues, wet tissues, napkins, wet napkins, towels, wet towels, masks, auxiliary cloths in masks, and hygiene products. Applicable to clothing, antiviral filter, antiviral air conditioner filter and so on.

  When the textile product for antiviral hygiene is a wiping material such as a tissue, wet tissue, napkin, wet napkin, towel, wet towel, etc., the anti-viral composition of the present invention of the above [Composition I] to [Composition IV] is used. The effect described in the item of the viral composition is expected. When the anti-viral sanitary textile is a wiping material such as tissue, wet tissue, napkin, wet napkin, towel, wet towel, etc., food, tableware, cooking utensils, fish, etc. The effect of inactivating non-enveloped viruses or enveloped viruses adhering to workers' fingers or instruments handling patient's filth is remarkable. And when alcohol is mix | blended [Composition I], in addition to this effect, the virus inactivation effect and bactericidal effect of alcohol are synergistically enhanced. When a surfactant is added [Composition II], in addition to this effect, the detergent cleaning effect and virus inactivation are synergistically enhanced. When an antibacterial agent is added [Composition III], in addition to this effect, the virus inactivation effect and bactericidal effect are synergistically enhanced. When a humectant is added [Composition IV], in addition to this effect, the virus inactivation effect of the wet type antiviral sanitary textile is further enhanced.

  When the antiviral sanitary textile is a mask, auxiliary mask in mask, sanitary clothing, antiviral filter, antiviral air conditioner filter, non-enveloped or enveloped virus that floats in the air, work or indoor environment Can be strongly inactivated, and infection to the human body can be prevented. When an antibacterial agent is added [Composition III], in addition to this effect, the virus inactivation effect and the bactericidal effect are synergistically enhanced. When a humectant is added [Composition IV], in addition to this effect, the virus inactivation effect of the wet type antiviral sanitary textile is further enhanced.

  The antiviral sanitary textile product of the present invention is preferably, for example, an in-mask auxiliary cloth. The in-mask auxiliary cloth is a disposable (replaceable) member that is used while being overlapped with a general mask in a portion covering the periphery of the mouth and nose. In the case of a general mask, although it is possible to capture the virus with the fibers that make up the mask and prevent the virus from entering the body, the captured virus has not lost its ability to infect, so contact it Then there is a risk of secondary infection. On the other hand, the auxiliary mask in the mask according to the present invention can lose the infectious ability of the trapped virus by the impregnated antiviral agent, for example, oyster extract or its processed product. By exchanging, secondary virus infection can be prevented efficiently and economically. In addition, there is flexibility that can be used in combination with various types of masks, for example, by combining a relatively coarse mask and an in-mask auxiliary cloth according to the present invention, while allowing easy breathing, It can be set as the mask provided with the high virus infection prevention effect.

  Hereinafter, the results of the antiviral test of the antiviral agent and the antiviral composition that can be used for the antiviral sanitary textile product of the present invention are shown as reference examples. If these antiviral agents and antiviral compositions are impregnated into paper, woven fabric or non-woven fabric, the antiviral sanitary textile product of the present invention can be obtained. In the following description (including the description in the table), “%” represents “% by weight” unless otherwise specified.

[Reference Example 1] Reference Example for Norovirus ・ Norovirus Test Solution The “norovirus test solution” used in the following Examples and the like was collected from a patient's stool and stored and frozen at −80 ° C. The solution was thawed on ice, 100 μL was taken, diluted 10-fold with phosphate buffered saline (PBS), cooled and centrifuged at 10,000 rpm for 20 minutes, and the precipitate was removed (950 μL solution).

-Real-time PCR method "Real-time PCR method" in the following examples and the like is a notification from the Director of the Food Safety Department of the Food Safety Department of the Ministry of Health, Labor and Welfare (No. 1105001 from the Food Safety Supervision, November 5, 2003. http: // (Refer to www.mhlw.go.jp/topics/syokuchu/kanren/kanshi/031105-1.html).

  In the real-time PCR method, the number of virus genomes in the states (a) and (b) in FIG. 1-1 is counted. That is, in the following examples and the like, it is evaluated as “effective” only when the norovirus (RNA) of (c) disappears, and even if the norovirus (RNA) of (b) does not disappear, infection・ Even if the growth ability is lost, it is evaluated as “no effect”. Nevertheless, the anti-norovirus composition of the present invention has been evaluated to have a higher effect than before, and the practical effect including the state of (b) norovirus is even higher. Conceivable.

[Reference Example 1-1]
[1] Preparation of oyster extract and ethanol preparation (a) Persimmon fermented liquid (FA-1): A pulverized immature fruit of persimmon (buckle) is put into a fine cloth bag and squeezed, and this squeezed Was put into a glass container and allowed to spontaneously ferment for about one year, and then the solid matter was separated by filtration to obtain a reddish brown solution. This solution contained about 10% solid component (oyster extract), and about half (about 5%) was oyster tannin.

  (B) Persimmon Shibu Freeze-dried Powder (FD-1): A pulverized immature fruit oyster (buckle) was put into a fine cloth bag and squeezed, and the squeezed freeze-dried powder was obtained. The yield was not constant because of natural products, but was about 5-7%.

  Using the persimmon extract (FA-1 and FD-1), ethanol, citric acid and trisodium citrate, compositions (ethanol preparations) A1 to A4 having the formulations shown in Table 1-1 were prepared. did. Moreover, the sample solution B1-B7 of the prescription similarly shown in Table 1-1 was prepared using the sample which mix | blended components other than the oyster extract.

[2] Anti-norovirus test 25 μL of the norovirus test solution and 25 μL each of the composition (A1 to A4 or B1 to B7) prepared as described above were mixed thoroughly, and the mixture was allowed to stand at room temperature for 2 minutes. And then diluted 50 times with PBS. After processing such as RNA extraction and cDNA synthesis using these diluted solutions, the number of virus genomic RNAs remaining in the diluted solution was counted by a real-time PCR method. Moreover, it tested similarly about the control with respect to each said sample solution. From the comparison of these measured values, the inhibition rate of norovirus was determined for each composition.

  The results are as shown in Table 1-1. Compositions A1 to A4 formulated with oyster extract (FD-1 or FA-1), compared to composition B1, which is a conventional general-purpose antibacterial agent and blended with popidone iodine that is recognized to have anti-norovirus activity The inhibition rate was significantly higher. In addition, compositions containing hydrolyzable tannin, low molecular weight tannin or related compounds showed no effectiveness against norovirus.

[3] Antibacterial test (1) Test sample Table 1 using oyster extract (FD-1) prepared as described above and glycerin-caproic acid ester, citric acid, trisodium citrate and ethanol. Compositions A5 to A7 having the formulation shown in -2 were prepared. Stock solutions of these compositions A5 to A7 and diluted solutions obtained by diluting them with purified water 2, 4, 8, 16 and 20 times were used as test samples.

(2) Types of bacteria E. coli ... IFO3301 strain Staphylococcus aureus ... IFO12792 strain (3) Medium and culture conditions E. coli ... Cultivated on desoxycholate agar medium at 35 ° C for 24 hours S. aureus ... 35 ° C on mannitol salt agar medium 48 hours of culture (4) Operation Add 1.0 mL of bacterial solution to each 9 mL of test sample, stir and sensitize for 1 minute, then smear on each selective medium, and check the breeding state after each culture time The effect was judged. The results are as shown in Table 1-3.

  Strong antibacterial properties were shown in the order of A7> A6> A5 against both E. coli and S. aureus. From these results, by using ethanol, fatty acid ester, and organic acid and salts thereof in combination, an anti-norovirus composition excellent in not only the effect on norovirus but also the effect on bacteria such as E. coli can be obtained. I understand that.

[Reference Example 1-2]
[1] Preparation of oyster extract and hand-washing foam / hand lotion agent (c) Oyster extract (Kojibu FD2-2): Koji that has been thoroughly sterilized (NaClO), washed, discolored (vitamin C), etc. The fruit was cut into dies and crushed. The obtained fruit / fruit juice solution is passed through a 200-mesh sieve, subjected to high-temperature sterilization (120 to 130 ° C., 7 to 10 seconds), and then freeze-dried to obtain a powdery oyster extract (Kojibu FD2-2) Was prepared.

  The components shown in Table 1-4 below were stirred and mixed to prepare three types of hand-washing foams containing the oyster extract (FD2-2). In addition, the components shown in Table 1-5 below were stirred and mixed to prepare a hand lotion containing the above oyster extract (FD2-2).

[2] Inactivation test of Norovirus 10 μL of the hand-washing foam and hand lotion prepared in Reference Example 1-2 above and 10 μL of Norovirus test solution were thoroughly mixed, and then 30 seconds and 60 seconds at room temperature. Or left for 120 seconds. In addition, 10 μL of total control (PBS (phosphate-buffered saline only)) was sufficiently mixed with 10 μL of Norovirus test solution and then allowed to stand at room temperature for 120 seconds. Collect 5 μL from these samples, dilute with 2 mL of PBS (400 times), perform RNA extraction, cDNA synthesis, etc., and then leave the virus genomic RNA remaining in the diluted solution by real-time PCR. Counted the number.

  On the other hand, as a control of the above sample, 5 μL each of the hand-washing foam and hand lotion prepared in Reference Example 1-2 was previously diluted with 4 mL of PBS, and then thoroughly mixed with 5 μL of Norovirus test solution, and the mixture was 120 at room temperature. Left for a second. These samples were also subjected to treatments such as RNA extraction and cDNA synthesis, and then the number of virus genomic RNA remaining in the diluted solution was counted by a real-time PCR method.

  The results are as shown in [FIG. 1-2]. Both the hand-washing foam and the hand lotion prepared in Reference Example 1-2 have been shown to have extremely high anti-norovirus properties.

[Reference Example 1-3] Stabilization test of anti-norovirus activity by vitamin C [1] The hand-washing foam (KSF15-2) and the hand lotion (KSYHL-5) prepared in Reference Example 1-2, respectively, 1% vitamin C added (KSF15-2 (VC) / KSYHL-5 (VC)) was prepared and kept under sunlight for 17 days from February 9 to 26, 2008 (clear weather). . About these 4 types of samples and total control, the inactivation test of norovirus was performed in the same procedure as the above-mentioned Reference Example 1-2 [2] (the contact time between the norovirus test solution and the sample was 2 minutes).

  The results are as shown in FIG. It can be seen that the anti-norovirus activity is maintained at a high level by addition of vitamin C for both the hand-washing foam and the hand lotion. The hand-washing foam did not have a significant decrease in activity even when vitamin C was not added, but the hand lotion lost its anti-norovirus activity by exposure to sunlight with no vitamin C added.

  [2] From oyster extract (said FD2-2) 0.3%, ethanol 50%, citric acid 1.6%, trisodium citrate 0.5%, glycerin monocaprate 0.5% and water (remainder) Composition (HA-72), oyster extract (said FD2-2) 0.3%, ethanol 50%, citric acid 1.6%, trisodium citrate 0.5%, glycerin monocaprate 0.5% A composition (HA-72 (VC)) consisting of 0.5% vitamin C and water (remainder) was prepared, and KSF15-2 (VC) and KSYHL-5 (VC) prepared in the above [1] were again prepared. Used and kept under sunlight for 10 days. These four types of samples were subjected to a norovirus inactivation test in the same procedure as in Reference Example 1-2 [2] (the contact time between the norovirus test solution and the sample was 2 minutes).

The results (average number of remaining noroviruses, n = 3) are as shown in FIG. Even when the composition takes the form of an ethanol preparation, it can be seen that anti-norovirus activity is maintained at a high level by adding vitamin C, as in the result of [1] above.

[Reference Example 1-4] Synergistic effect test of anti-norovirus activity with citric acid Oyster extract (FD2-2) 0.3%, ethanol solution A consisting of 50% ethanol and water (remainder), oyster extract (above FD2-2) Prepare ethanol solution B consisting of 0.3%, citric acid 1.6%, ethanol 50% and water (remainder), and total control, according to the method of Reference Example 1-2 [2] (Norovirus test solution and sample contact time was 2 minutes) Norovirus inactivation test was performed.

  The results are as shown in FIGS. 1-5. When the control was set to 100, the residual ratio of norovirus was 6.7 for ethanol solution A and 0.08 for ethanol solution B. By adding citric acid to the oyster extract and ethanol, the anti-norovirus activity was further increased. It turns out that it becomes remarkable.

[Reference Example 2] Reference examples for non-enveloped viruses other than Norovirus
Preparation of oyster extract (a) Persimmon astringent fermented liquid (for HA-23): Put immature fruit pulverized oysters (astringent persimmon) into a fine cloth bag and squeeze it into a glass container. After natural fermentation for about one year, the solid matter was filtered off to obtain a reddish brown solution. This solution contained about 10% solid component (oyster extract), and about half (about 5%) was oyster tannin.

  (B) Strawberry astringent FD powder (for HA-72A / HA-201): Dice the immature fruit of the cut cocoon, which has been thoroughly sterilized (NaClO), washed, and prevented from discoloration (vitamin C). Cut into a shape and crushed. The obtained fruit / fruit juice solution is passed through a 200 mesh sieve, centrifuged, subjected to high-temperature sterilization (120-130 ° C, 7-10 seconds), and then freeze-dried to prepare a powdery oyster extract did.

Preparation of Reagents Reagents according to the composition shown in Table 2-1 were prepared and used for verification of effects on fish disease viruses or human pathogenic viruses described below (Reference Examples 2-1 to 2-5).

[Reference Example 2-1] Verification of effects on fish disease virus (1) Viruses used The characteristics of viruses used are shown in Table 2-2. Viral neuronecrosis, caused by viral neuronecrosis virus (NNV) (Schneemann et al., 2005), is a disease that occurs in various marine fish and has a very high mortality rate. Aquavirnavirus (ABV) (Nguyen et al., 1994) is a spherical virus with double-stranded RNA as a genome, causing infectious pancreatic necrosis in fish species such as brook trout, rainbow trout, eel, and flounder. The red sea bream virus (RSIV) (Natsushima Nakahiro and Kurita Jun, 2005) is the causative agent of the red sea bream virus disease. In recent years, not only red sea bream, but also many fish species such as yellowtail, amberjack, sea bass, etc. ing. Among the seafood diseases in Japan, this disease is one of the most frequently reported damages.

(2) Mixing of virus and test reagent Among the reagents prepared according to the above composition, those listed in Table 3 were diluted with Hanks' balanced salt solution (Nissui) (HBSS), and the oyster extract was extracted. Alternatively, the concentration was adjusted to 0.3% or 0.03% of the control. 50 μL of these virus solutions were mixed with 50 μL of these reagents (the final concentration of the oyster extract or control was 0.15% or 0.015%), and the mixture was allowed to stand at room temperature for 2 minutes. Thereafter, the reaction was stopped by adding 9.9 mL of HBSS, and a 10-fold dilution series of these samples was prepared using HBSS.

(3) Virus titration test using cultured fish cells Each cultured cell shown in Table 2-2 is seeded in a 96-well plate (IWAKI) at 80% confluent, and 90 μL of medium is added to each well. In addition, the cells were cultured for 24 hours. The media used were L-15 (for E-11), MEM (for RTG-2) and BME (for GF). Thereafter, 10 μL of the previously prepared dilution series was inoculated into each well and further cultured for 10 days to confirm the appearance of cytopathic effect (indicator of virus infection). The limiting dilution point at which no cytopathic effect was observed was confirmed, and the virus titer was measured according to the method of Reed and Muench (1938). The ratio of the virus titer of the test group to the virus titer (100%) of the control group was calculated to evaluate the antiviral activity.

(4) Experimental results The antiviral action of each reagent is as shown in Table 2-3.

[Reference Example 2-2] Verification of effects on human pathogenic virus (Coxsackie virus) (1) Test virus Coxsackievirus group B type 5 (non-enveloped virus, clinical isolate, 2-year-old girl, aseptic meningitis-derived FL cell passage 2 Generation)
(2) Virus infectivity titration method
Infect FL cells and calculate 50% infectious dose (unit: 50% tissue culture infectious dose [TCID50]) using the Behrens-Kaerber method.

  FL cells: A mixed cell line of HeLa cells (derived from human cervical cancer epithelial cells).

(3) Test method In order to eliminate the influence of serum contained in the original virus, the virus solution was diluted 50 times with PBS in advance. Mix this diluted virus with the reagents listed in Table 4 among the reagents prepared according to the above composition, PBS and 50% (v / v) ethanol each in equal amounts (100 μL) and allow to react at room temperature for 3 minutes. After that, a 10-fold dilution series was made with the diluted solution, inoculated into cells in a 96-well plate, and several days later, the spread of CPE was observed, and the virus infectivity titer was measured.
Diluent: Dulbecco's modified Eagle's minimum essential medium, penicillin G, streptomycin, amphotericin B (serum-free)
(4) Results The antiviral action of each reagent is as shown in Table 2-4 and FIG. 2-1. With HA-72A and HA-201 (oyster extract), the coxsackie virus activity titer was suppressed to 30,000 to 1 / 50,000 or more, and strong antiviral activity was observed. On the other hand, such activity was not observed with other reagents. From this, it is considered that oyster extract is the main antiviral activity, and citric acid, ethanol and the like do not have anti-coxsackievirus activity. HA-72A and HA-201 seem to remain virus-infectious after treatment, but this is the level of cytotoxicity caused by the reagent itself, and is not necessarily a change caused by the virus. There is no contradiction to thinking that the virus is almost completely inactivated.

[Reference Example 2-3] Verification of effects on human pathogenic virus (adenovirus) (1) Test virus Adenovirus (Non-proliferative adenovirus expressing herpesvirus and thymidine kinase derived from non-enveloped virus, adenovirus type 5)
(2) Test method Test method Mix virus solution and drug in equal amounts (3.5 μL), react at room temperature for 3 minutes, and then make a 10-fold dilution series with the diluted solution. FL cells on the coverslip The virus infectivity titer was measured after inoculation.
Diluent: Eagle's minimum essential medium, penicillin G, streptomycin (serum-free)
(3) Method for measuring virus infectivity titer Monolayer FL cells [HeLa cells (derived from human cervical cancer epithelial cells) mixed cells] on coverslips (9 x 28 mm) were washed once with PBS and 50 μL of virus was obtained. The solution is infected, washed once with PBS after adsorption for 1 hour, and added with a cell maintenance solution (same as the diluted solution) and cultured.

  Two days later, fluorescent staining is performed with an anti-adenovirus antibody (monoclonal antibody: Chemicon MAB805) and Alexa Fluor 488-labeled anti-mouse IgG antibody (SantaCruz Biotechnology), and positive cells in the field are counted under a fluorescence microscope (400 times). (Calculate the average value for 20 fields.) Based on the area of the field, convert the number of positive cells per coverslip and calculate the infectivity per ml of virus solution (unit is CIU (cell infecting units)). / ml; Kiyotani et al., Virology 177: 65-74, 1990).

  In FL cells, the adenovirus used synthesizes viral proteins after the initial infection, but cannot produce progeny viruses and the virus does not spread to neighboring or surrounding cells. Thus, a positive cell corresponds to at least one infectious virus. Thereby, the infectivity titer of the original virus solution can be measured. An actual fluorescence microscope image is shown in FIG. In 293T cells, this virus is capable of multi-stage growth and can be observed to spread to neighboring cells.

(4) Results The antiviral action of each reagent is as shown in Table 2-5 and FIG. 2-3.

  HA-72A had strong inactivation activity against adenovirus. 50% ethanol, citric acid + 50% ethanol (HA-202) has no inactivation ability, but 1% nebulization + 50% ethanol (HA-201) suppressed adenovirus infectivity, so It is thought to be the main antiviral activity against adenovirus. In addition, with other reagents, tannic acid and catechin had the same strong virus inactivating activity.

  In this experiment, a gene-deficient non-proliferative adenovirus was used in place of adenovirus, but this lacked part of the gene, but the particle structure is not different from that of normal viruses, and adsorbed and invaded viruses. There seems to be no difference in the process. Therefore, the results of this experiment are considered to apply to ordinary adenoviruses.

  From the results shown in Table 2-5 and FIG. 2-3, it can be seen that the anti-adenovirus effect is remarkable in scabies, tannic acid and catechin.

[Reference Example 2-4] Verification of effect on feline calicivirus (1) Test virus Feline calicivirus (non-enveloped virus, CRFK cell passage 2 passage, distributed by National Institute of Food Sciences) History is unknown.)
(2) Cells
CRFK cells: Feline kidney-derived cultured cells. Distributed from National Institute of Pharmaceutical Health. (It is assumed that the cells are the same as ATCC CCL-94.)
(3) Measurement of virus infectivity titer In order to eliminate the influence of serum contained in the original virus, the virus solution is diluted 5 times in advance with a diluent (Eagle's minimum essential medium, penicillin G, streptomycin). The diluted virus and drug were mixed in equal amounts (35 μL each) and reacted at room temperature for 3 minutes, and then a 10-fold serial dilution series was prepared with the diluted solution.

  Monolayer CRFK cells in a 96-well plate were washed once with PBS and inoculated with diluted virus solution (50 μL / well). After incubating with a carbon dioxide incubator for 1 hour for virus adsorption / invasion, the cells were washed once with PBS, added with a cell maintenance solution (same as the diluent) (100 μL / well), and cultured. After 3 days, the CPE spread and fixed and stained, and the 50% infection dose (unit: 50% tissue culture infectious dose [TCID50]) was calculated using the Behrens-Kaerber method, and the virus infection titer was measured.

  An example of the plate after fixation / staining is shown in FIG. 2-4, and the infectious titer calculated therefrom is shown below. (The effect of adding serum to the cell maintenance solution in a preliminary experiment was examined.)

(4) Results The antiviral action of each reagent is as shown in Table 2-6 and FIG. 2-5.

  In this experiment, the time to inoculate the virus solution was limited to only 1 hour to minimize the toxic effects of the drug on the cells. I was injured. An injury up to 10-1 dilution corresponds to an infection titer of 3.2 x 101TCID50 / ml, assuming that it is due to viral infection. Below this value, it was impossible to judge whether the result was due to drugs or viruses, and the experiment was meaningless, so a graph was created with this value as the lower limit.

  HA-72A and HA-201 (Hakubushi) showed strong suppression of infectious titer, and it was confirmed that Hakuri has a feline calicivirus inactivating effect. On the other hand, HA-202 (citric acid), HA-19 (propyl gallate), HA-20 (green tea tannin), and HA-21 (pyrogallol) also showed a virus suppressing effect.

  The virus was inactivated with HA-202 (citric acid), but not with HA-16. This experiment was repeated and confirmed to be reproducible. When the pH of the reagent was measured, HA-202 was slightly different from pH 4.0 and HA-16 to 4.20 (Table 2-7).

  Such a difference in pH may be significant for feline calicivirus inactivation. In general, feline calicivirus is a respiratory virus and is highly sensitive to acids.

[Reference Example 2-5] Verification of effects on rotavirus (1) Virus used Rotavirus Wa strain Received a share from Dr. Mitsutaka Kuzuya, Okayama Prefectural Environmental Health Center. It was passed from the Ehime Prefectural Institute of Health, Mitsuaki Oseto, for 88 generations at the Okayama Prefectural Environmental Health Center.

  In addition, rotavirus (Rotavirus) is a non-enveloped virus having a segmented double-stranded RNA belonging to the genus Rotavirus belonging to the reoviridae family (diameter 60-80 nm), and causes acute gastroenteritis. Especially in infants, it causes winter diarrhea (called white diarrhea from white rice-like stool-like stool).

(2) Cells
MA104 cells (derived from African green monkey fetal kidney, epithelial) were distributed by Dr. Mitsutaka Katsuraya, Okayama Prefectural Environmental Health Center. At the time of distribution, it was passed for 55 generations at the Okayama Prefectural Environmental Health Center. The cells were cultured in Eagle's minimum essential medium (MEM) containing 10% fetal bovine serum (FBS).

(3) Virus infectivity titration method Equivalent amounts of virus solution and drug (35 μL) are mixed, reacted at room temperature for 3 minutes, and then diluted 10-fold with dilution solution (MEM) to create a coverslip ( 9 × 28 mm) of MA104 cells were infected with 50 μL of the virus solution, adsorbed for 1 hour, washed once with PBS, added with growth medium (10% FBS-MEM) and cultured.

  One day later, fluorescent staining was performed with an anti-rotavirus antibody (monoclonal antibody: Abgene REMEI-1) and Alexa Fluor 488-labeled anti-mouse IgG antibody (SantaCruz Biotechnology), and positive cells in the field were counted under a fluorescence microscope (400x) did. (Average value of 20 fields) Based on the area of the field, the number of positive cells per coverslip was calculated, and the infectivity titer per ml of virus solution was calculated (unit is CIU (cell infecting units) / ml; Kiyotani et al., Virology 177: 65-74, 1990). When there were few positive cells, the whole coverslip was observed and the positive cells were counted.

  Rotavirus requires trypsin for multistage growth. In this measurement, trypsin is not added, so the virus from the first infected cell cannot infect the neighboring cells. Therefore, one positive cell corresponds to almost one infectious virus. Thereby, the infectivity titer of the original virus solution can be measured. The actual fluorescence image is shown in Fig. 2-6.

(4) Results The antiviral action of each reagent is as shown in Table 2-8. Rotavirus was inactivated by all of the nephew, pentagalloyl citrate glucose, wattle tannin, coffee tannin, propyl gallate, green tea tannin and pyrogallol (each + 50% ethanol). Inactivation with 50% (v / v) ethanol alone was incomplete, but it was almost completely inactivated when the ethanol concentration was increased to 80% (v / v).

  The antiviral activity of various reagents against Coxsackievirus, adenovirus, feline calicivirus and rotavirus in the above Reference Examples 2-2 to 2-5 is summarized in the following table.

[Reference Example 3] Reference Example for Envelope Virus [Reference Example 3-1]
Prepare drugs according to the formulation shown in Table 3-1, and for human influenza virus, avian influenza virus, vesicular stomatitis virus as it is, herpes simplex virus type 1 and Newcastle disease virus are further halved with water. Diluted and used in the following examples. As a control, phosphate buffered saline (PBS) was used. In addition, persimmon astringent FD powder (oyster extract) is sterilized by cutting the immature fruit of the persimmon that has been thoroughly sterilized (NaClO), washed, discoloration-prevented (vitamin C), etc. The obtained fruit / fruit juice solution was passed through a 200 mesh sieve, centrifuged, subjected to high temperature sterilization treatment (120 to 130 ° C., 7 to 10 seconds), and then prepared by freeze drying treatment.

  The virus solution of each test virus shown in Table 3-2 above and the drug or control shown in Table 3-1 above were mixed in an equivalent amount (35 μL each) and reacted at room temperature for 3 minutes, and then DMEM A 10-fold serial dilution series was prepared using (Sulbecco's modified minimun essential medium). The diluted virus solution was inoculated into a monolayer culture cell of a 96-well plate (100 μL / well) and cultured. After 4 days, the CPE spread and fixed and stained, the 50% infection dose (unit: 50% tissue culture infections dose [TCID50]) was calculated using the Behrens-Kaerber method, and the virus infection titer was measured. The results are as shown in Table 3-3 and FIGS. 3-1 to 3-5.

  All of the above five viruses are hardly affected by 20% or 10% (w / w) ethanol, but the infectivity titration is greatly reduced when blended with persimmon astringent FD powder, wattle tannin, or green tea tannin. Antiviral activity was confirmed. This result indicates that each of these substances is an excellent active ingredient against a wide range of enveloped viruses.

  Moreover, although pentagalloyl glucose was somewhat weak against vesicular stomatitis virus, antiviral activity against each virus was confirmed. Pyrogallol was also confirmed to have antiviral activity against four types of viruses except Newcastle disease virus. Furthermore, glycerin monocaprate was confirmed to have antiviral activity against all of the above five viruses, and citric acid / trisodium citrate was confirmed to have antiviral activity against four viruses excluding Newcastle disease virus.

  On the other hand, coffee tannin showed almost no antiviral activity against any of the above five viruses. Propyl gallate showed some antiviral activity depending on the type of virus.

[Reference Example 3-2]
Viral hemorrhagic septic virus (VHSV) causes hemorrhagic sepsis in trout, but this disease has been confirmed recently in flounder and the like (Takano et al., 2001).

  The test reagents are shown in Table 3-4. Each test reagent was adjusted to a concentration of 0.2% using Hanks' balanced salt solution (Nissui) (HBSS). To 50 μL of the reagent, 50 μL of various virus solutions were mixed (final reagent concentration 0.1%) and allowed to stand at room temperature for 2 minutes. Thereafter, the reaction was stopped by adding 9.9 mL of HBSS, and a 10-fold dilution series of these samples was prepared using HBSS.

  In the virus titer measurement test, FHM fish cultured cells (derived from Pimephales promelas) were first seeded in a 96-well plate (IWAKI) to 80% confluent, and 90 μL of the medium was added to each well and cultured for 24 hours. The medium used was MEM. Thereafter, 10 μL of the previously prepared dilution series was inoculated into each well and further cultured for 10 days to confirm the appearance of cytopathic effect (indicator of virus infection). The limiting dilution point at which no cytopathic effect was observed was confirmed, and the virus titer was measured according to the method of Reed and Muench (1938). The ratio of the virus titer of the test group to the virus titer (100%) of the control group was calculated to evaluate the antiviral activity (Table 3-5).

  For viral hemorrhagic sepsis virus, fermented koji juice (oyster extract), pentagalloyl glucose, wattle tannin, green tea tannin (catechins) and pyrogallol are highly effective, and these are excellent active ingredients. It has been shown that In addition, gallic acid and pentaploid tannin showed similar effects, and coffee tannin showed a certain effect.

[Reference Example 3-3]
(1) Drugs used
HA-72A (see Reference Example 2) and KSF15-2 (see Reference Example 1-2), and phosphate buffered saline (PBS) as a control
(2) Virus used Avian influenza virus A / swan / Shimane / 499/83 (H5N3)
(3) Cells
MDCK (+) cells
(Canine renal tubular epithelial cell origin: Noma K et al. Arch. Virol. 143: 1893-1909, 1998.)
(4) Virus infectivity titration method Equal volumes of virus solution and drug (35 μL each) were mixed, reacted at room temperature for 3 minutes, and 10-fold serial dilutions were prepared with DMEM (Dulbecco's modified minimum essential medium). . The diluted virus solution was inoculated into a monolayer culture cell of a 96-well plate (100 μL / well) and cultured. After 4 days, when the CPE spread, the cells were fixed and stained, the 50% infectious dose (unit: 50% tissue culture infectious dose [TCID50]) was calculated using the Behrens-Kaerber method, and the virus infectivity titer was measured.

  To examine the treatment time, the virus solution and the drug were mixed, and after a predetermined time (10, 30, 60 seconds), diluted with DMEM 500 times to stop the reaction with the drug. Was measured. The obtained infectious titer was corrected to 500 times.

(5) Results Action of drug:
In both HA-72A and KSF15-2, the virus activity decreased below the detection limit after 3 minutes of treatment (see Table 3-6 and FIG. 3-6). Since both drugs are toxic to cells to some extent, the result of “3.2E + 03 TCID50 / ml” is the limit for detecting the action of the virus.

Effect of processing time:
Since it is diluted 500 times, 1.6.E + 04 TCID50 / ml is the detection limit of virus activity (see Table 3-7). It is thought that there is.

  As described above, both reagents are inactivated (more than about 3000 times) so that the avian influenza virus cannot be detected. Also, it is considered effective at least for 10 seconds.

[Reference Example 3-4]
(1) Drugs used
HA-72A (see Reference Example 2) and KSF15-2 (see Reference Example 1-2), and phosphate buffered saline (PBS) as a control
(2) Viruses and cells used

(3) Method for measuring virus infectivity titer Equal amounts of virus solution and drug (35 μL each) were mixed and reacted at room temperature for 3 minutes, and then a 10-fold serial dilution series was prepared with PBS. The diluted virus solution was inoculated into a monolayer culture cell of a 96-well plate (50 μL / well), and virus adsorption was performed for 30 minutes. Thereafter, the virus inoculum was removed by suction, and DMEM, 20 μg / ml trypsin (100 μL / well) was added. Six days later, when the CPE spread, the cells were fixed and stained, and a 50% infectious dose (unit: 50% tissue culture infectious dose [TCID50]) was calculated using the Behrens-Kaerber method, and the virus infectivity titer was measured.

(4) Results and discussion
Both HA-72A and KSF15-2 highly inactivated the H1N1 virus (see Table 3-9 and FIG. 3-7). Although the infection value of the original virus was not so high, it was the result that it was suppressed to 0.06% or less, but it may actually be highly inactivated.

[Reference Example 3-5]
(1) Manufacturing method of aqueous juice liquid After washing and drying 330 g of raw material blue koji (chiba prefecture Chimoku rice, collected on August 22, 2009) to remove koji, the seeds were removed at the same time as cutting to about 2 cm square. (280 g). This was taken in a mixer and sufficiently ground with about 2.8 g (1%) of L-ascorbic acid (vitamin C) and 280 ml of purified water over about 3 minutes. This was squeezed several times with a non-woven fabric and heat-sterilized at 70-80 ° C. for about 30 minutes to obtain a pale yellow liquid (460 g). When this aqueous squeezed juice (processed oyster extract) was placed in a sealed container and placed at room temperature, it could be stored for a long period (at least 3 months) without producing off-flavors, discoloration, or precipitation.

Sugar content of strawberry flesh 9.4% (sugar content meter ATAGO Pal-1)
Tannin content 2.2% (quantitative Folin-Denis method)
(2) Manufacturing method of ethanolic juice After washing and drying 300 g of raw rice koji (Chiba Prefecture Hyakumoku, collected on August 22, 2009) to remove the koji, the seeds were removed at the same time as cutting to about 2 cm square. (260 g). This was taken up in a mixer and pulverized in about 3 portions with 2.6 g (1%) of L-ascorbic acid (vitamin C) and 260 ml (210 g) of 95% ethanol. When this was squeezed several times with a nonwoven fabric, a pale yellowish green liquid was obtained (430 g). When this ethanolic juice (treated product of oyster extract) was placed in a sealed container and placed at room temperature, it could be stored for a long period (at least 3 months) without causing off-flavors, discoloration, or precipitation.

Sugar content 9.5% (sugar content meter AYAGO Pal-1)
Tannin content 2.4% (quantitative Folin-Denis method)
[Example 1]
(1) Drugs used
Nonwoven fabric soaked in 5% wrinkle solution (in 50% (w / w) ethanol) or 1%, 0.1%, 0.01%, 0.001%, 0.0001% wrinkle solution. As a control, a non-woven fabric soaked in 50% (w / w) ethanol (0% wrinkle solution) was used. In addition, the “boiled shibu” used in this example is the “bamboo shibu FD powder (oyster extract)” used in Reference Example 3-1.

(2) Test virus Avian virus-derived influenza virus A / swan / Shimane / 499/83 (H5N3), which was purified by sucrose density gradient centrifugation after growth on growing eggs and dialyzed against PBS .

(3) Cells
MDCK (+) cells (from canine renal tubular epithelial cells: Noma K et al. Arch. Virol. 143: 1893-1909, 1998.)
(4) Experimental method (i) Pretreatment of cloth Nonwoven fabric soaked in the above-mentioned medicine for about 60 minutes was dried for 1 day or more in a sterile environment in a safety cabinet to fix tannin. In order to remove the unfixed excess tannin, it was washed 4 times with sterilized water and once with sterilized PBS, and then used again in the dried or semi-dried state for the experiment.

(Ii) Reaction of non-woven fabric and virus Cut the cloth into an appropriate size (about 20 mg for dry cloth, about the same size as dry cloth for semi-dry cloth) and place it on a 6-well plate or 12-well plate. placed. Soak 10 μL, 50 μL, or 100 μL of virus solution in a non-woven fabric, react for 30 minutes in a carbon dioxide incubator, and then add an excess amount (300 μL, 500 μL, 1000 μL) of DMEM (Dulbecco's modified minimum essential medium). Virus was eluted by petting. The amount of virus solution or the amount of DMEM used for elution is the same as that of the control in one experiment.

(Iii) Method for measuring virus infectivity titer A 10-fold serial dilution of the eluted virus was prepared using DMEM. The diluted virus solution is inoculated into a monolayer culture cell of a 96-well plate (50 μL / well), adsorbed in a carbon dioxide incubator for 30 minutes, and the virus inoculum is removed by suction and DMEM containing 20 μg / ml trypsin ( 100 μL / well) was added and cultured. After 4 days, the CPE spread and fixed and stained, the 50% infection dose (unit: 50% tissue culture infectious dose [TCID50]) was calculated using the Behrens-Kaerber method, and the virus infection titer was measured.

(5) Results (i) Action of glazed treated non-woven fabric Compared with the original virus solution, the control (0% glazed treated) non-woven fabric had a considerable decrease in the infectious value of the virus, but 5% 柿Further reduction of the residual virus was observed in the splash-treated nonwoven fabric (about 1/1000 of the original virus solution, about 1/20 of the control nonwoven fabric, see Table 4-1 and FIG. 4-1). It is thought that the influenza virus was inactivated by phlegm treatment.

(Ii) Effect of haze concentration
The virus infectivity value of the 1% wrinkle-treated non-woven fabric was reduced to about 1/20 compared to 0% wrinkle-treated non-woven fabric, but no decrease in the virus infectivity value was observed at a concentration of 0.1% or less (Table 4- 2 and FIG. 4-2).

(6) Consideration A decrease in the infectious value of influenza virus was observed due to the glazed non-woven fabric. In addition, the treatment with 1% scum was effective, but the treatment with 0.1% or less of scab was less infectious compared to treatment with 50% (w / w) ethanol alone. No decrease in value was observed.

  However, the above-mentioned examples are merely examples of the present invention, and the oyster extract or the oyster extract or its depending on the target virus, the type of fibrous base material, or the composition of the composition impregnated into the fibrous base material It will be appreciated by those skilled in the art that the appropriate dosage of the treatment can also vary.

Claims (13)

At least one fibrous substrate selected from the group consisting of paper, woven fabric and non-woven fabric,
It is obtained by inactivating the enzyme derived from the plant of the genus Diospyros which was contained in the juice or extract of the fruit of the genus Diospyros plant containing tannin by heating or treating with alcohol. An antiviral agent against an influenza virus of the genus Influenza A, comprising an obtained oyster extract processed product as an active ingredient ,
One or more components of alcohol, surfactant, antibacterial agent (excluding ethanol, organic acid and its salt) and moisturizer
A antiviral hygiene textile impregnated with the antiviral composition containing,
The textile product for antiviral hygiene, wherein the processed product of oyster extract (in terms of solid content) is 1 to 5% by weight based on the whole antiviral composition .   The anti-viral sanitary textile product according to claim 1, wherein the processed product of oyster extract contains at least condensed tannin.   The antiviral sanitary textile product according to claim 1 or 2, wherein the plant of the genus Oyster is Diospyros kaki. The antiviral agent is at least selected from the group consisting of catechins, wattle tannins, pentagalloyl glucose , pyrogallol , and organic acids and / or salts thereof (except those in the processed oyster extract). The antiviral sanitary textile product according to any one of claims 1 to 3, comprising one kind as an active ingredient. The textile product for antiviral hygiene according to any one of claims 1 to 4 , which targets human influenza virus or avian influenza virus as the envelope virus. The textile product for antiviral hygiene according to any one of claims 1 to 5 , impregnated with an antiviral composition containing at least the antiviral agent according to any one of claims 1 to 5 and an alcohol. . The antiviral hygiene according to any one of claims 1 to 5 , impregnated with an antiviral composition containing at least the antiviral agent according to any one of claims 1 to 5 and a surfactant. Fiber products. At least, antiviral agents and antimicrobial agent according to any one of claims 1 to 5 (ethanol, excluding the organic acids and salts thereof.) And impregnated with the antiviral composition containing, claim 1-5 An antiviral sanitary textile product according to any one of the above. The antiviral sanitary fiber according to any one of claims 1 to 5 , impregnated with an antiviral composition containing at least the antiviral agent according to any one of claims 1 to 5 and a humectant. Product. The textile product for antiviral hygiene according to any one of claims 6 to 9 , wherein the antiviral composition further contains vitamin Cs. The antiviral composition contains an organic acid and / or a salt thereof (excluding those in the processed oyster extract) in such an amount that the pH of the composition is 6.0 to 2.0. The textile product for antiviral hygiene according to any one of claims 6 to 10 . The textile product for antiviral hygiene according to any one of claims 6 to 11 , which takes the form of a tissue, a wet tissue, a napkin, a wet napkin, a towel or a wet towel. The textile product for antiviral hygiene according to any one of claims 6 to 11 , which takes the form of a mask, an auxiliary cloth in the mask, sanitary clothing, an antiviral filter or an antiviral air conditioner filter.