KR20090127110A - Alcoholic disinfectant containing green tea extract - Google Patents

Abstract

PURPOSE: A disinfectant containing green tea extract is provided to ensure effective moisturization and skin protection and remove specific alcohol smell. CONSTITUTION: An alcoholic disinfectant contains green tea ethanol extract. The disinfectant contain 0.05-1.5 weight% of polyphenol, 40-75 weight% of ethanol, 2-10 weight% of isopropanol, and 20-40 weight% of purified water. A method for producing the disinfectant containing green tea ethanol extract comprises: a step of washing and drying green tea leave; a step of extracting green tea leave with 40-80% of ethanol at 20~40°C for three to five hours; a step of filtering the extract and concentrating; and a step of mixing the green tea extract with 0.05-1.5 weight% of polyphenol, 40-75 weight% of ethanol, 2-10 weight% of isopropanol, and 20-40 weight% of purified water.

Description

Alcoholic disinfectant with green tea extract {Alcoholic disinfectant containing green tea extract}

The present invention relates to an alcoholic bactericidal disinfectant containing green tea extract.

Although the disinfectant disinfectant has attracted the public's attention due to the H1N1 flu, disinfectant disinfectant is essential for people who are interested in hygiene and health in addition to some professional occupations in the past. To be able to prevent the swine flu as well as most infectious diseases has become more essential knowledge than common sense for most people. There are many functions of disinfectant disinfectants, but the main function is to remove bacteria, and most of the causative agents of bacteria are removed within 15 seconds by using disinfectant disinfectants. In addition, moisturizing function that prevents the skin from drying and non-toxic ingredients that are not irritating to the skin protects the skin and can easily disinfect hands anytime and anywhere without water and soap.

Therefore, a method for producing a disinfectant disinfectant is known, and in particular, the Republic of Korea Patent Registration No. 439435, a lotion type skin protective disinfectant, consisting of ethanol, isopropyl alcohol, benzalkonium chloride, skin moisturizer, Korean Patent Publication No. No. 2007-119970, a hand sanitizer containing nano silver consisting of ethanol, isopropyl alcohol, benzalkonium chloride, skin moisturizer, etc., and Korean Patent Registration No. 456133, ciprofloxacin, clofloxacin, enoxacin, Known for sterilization composition containing ingredients such as Salvia ginseng, but using a range of concentrations of ethanol, surfactants, skin moisturizers, etc. The alcohol is in direct contact with the skin after skin damage, alcohol Odors may be offensive to the user.

Green tea is processed from the leaves or leaves of Camellia sinensis O. Kuuntze, which belongs to the Camellia family. Green tea contains various ingredients such as catechin, caffeine, amino acids, fiber, pectin, and vitamins. These components have various effects such as antioxidant activity, antibacterial effect, inflammatory prevention effect, deodorant effect, astringent effect, caries prevention effect, and anti-aging effect of skin damage caused by UV rays. Since catechins have affinity for proteins, they enhance the cleansing effect and skin moisturizing ability on contaminated stratum corneum, inhibit bacteria, and remove body odor. Since human skin is exposed to free radicals, sunlight, bacteria and pollutants, it is oxidatively damaged by free radicals and free radicals to promote aging. Green tea catechins have strong antioxidant activity, effectively protecting skin. It is known. In particular, EGCg, one of the catechins, has been reported to promote regeneration and skin cell differentiation of aged skin as the most contained in green tea.

Therefore, a method of manufacturing an antimicrobial composition using green tea is known, and in particular, Korean Patent Publication No. 2003-90212 discloses an antimicrobial composition comprising an active ingredient extracted with green tea polyphenols with water, methanol, and ethanol, and a Korean patent. Publication No. 2009-66651 discloses that it can increase the bactericidal power and antimicrobial composition composed of hydrogen fluorocarbon, ethanol, grapefruit extract, green tea extract, etc., and uses a deodorant to remove odors peculiar to ethanol. No. 2003-37094 discloses a grapefruit extract, green tea extract, and citric acid in alcohol or alcohol / water mixed solvents, which are effective in food preservation and antimicrobial activity against common bacteria, food poisoning bacteria, and fungi, and Korean Patent Registration No. 890068. Fermented liquid extracts and additive mixtures of pine needles, onions, green tea, and grapes It is known that green tea is effective for moisturizing the skin as a gelled skin moisturizing composition, and it is not well known that green tea extract removes ethanol-specific odor.

The inventors of the present invention, while continuing to study the removal of ethanol-specific odor in the preparation of disinfectant disinfectant, and confirmed that the green tea extract is excellent in the sterilization disinfection during the hand disinfection, as well as the skin protection effect and the effect of removing the odor unique to ethanol The invention has been completed.

Therefore, the problem to be solved by the present invention is to provide a disinfectant disinfectant containing green tea extract that is effective in strengthening disinfection and sterilization during hand disinfection, as well as skin protection, alcohol and body odor removal.

Furthermore, an object of the present invention is to provide a method for producing the green tea extract containing disinfectant.

Green tea extract-containing alcoholic disinfectant according to the present invention for solving the above problems include green tea extract, isopropanol and the like extracted with ethanol.

The green tea extract included as an active ingredient in the bactericidal disinfectant of the present invention is not particularly limited as long as it is extracted with green tea using an ethanol extraction solvent, and may be an extract or an extract solid obtained by concentrating and drying the extract. Although extraction may be performed by one extraction operation, extraction operation may be performed multiple times using another solvent as needed.

The ethanol aqueous solution, which is the extraction solvent, is extracted using an aqueous 30 to 90% ethanol solution, and preferably extracted with an aqueous 40 to 80% ethanol solution. The ethanol is preferably edible alcohol ethanol. If the concentration of ethanol is less than 40%, there is a risk that microorganisms remain in the extract, and if the concentration of ethanol is 80% or more, the extraction rate of total polyphenols such as catechin, which is a major component of green tea, is lowered.

Extraction time is 30 minutes to 5 hours, preferably 3 to 5 hours extraction is good, extraction time is 30 minutes or less extraction rate is reduced, if more than 5 hours extraction rate does not increase any more.

Extraction temperature is 10 ~ 60 ℃, preferably extracted at 20 ~ 40 ℃, extraction temperature is less than 10 ℃ cost increase factors such as chiller and the extraction rate of green tea components is lowered, 60 ℃ or more extraction time is shortened but extract There is a browning problem.

The extraction operation is also not particularly limited and may be performed according to a conventional method. In order to improve extraction efficiency, it can extract even if it uses the extractor provided with shaking extraction, a stirrer, etc. For example, an extraction treatment is performed by separating or treating the treatment liquid into an extract and an extraction residue by filtration, centrifugation, or decantation, by carrying out an extraction treatment which is stirred or shaken with the extraction solvent without being immersed in or immersed in the green tea extraction solvent. The extraction residue may further be subjected to the same extraction treatment. The obtained extract may be used as it is, but may be further concentrated and / or fractionated if necessary.

The total polyphenol content in the preparation of the disinfectant disinfectant with green tea ethanol extract to include 0.05 ~ 5% by weight, preferably 0.05 ~ 1.5% by weight. If the total polyphenol concentration is less than 0.05% by weight, synergistic effects such as deodorization and skin protection by the green tea components are lowered. If the total polyphenol concentration is 5% by weight or more, there is a problem in that the cost increase and the green tea components are excessively remaining.

In the green tea extract-containing alcoholic disinfectant of the present invention, ethanol is suitable for 40-75% by weight for the sterilization effect, the sterilization power is lowered if the 40% by weight or less than 75% by weight.

The isopropanol is used for the purpose of enhancing the sterilization effect by ethanol, in the present invention, isopropanol includes 2 to 10% by weight, and isopropanol is more than 10% by weight, resulting in rejection due to peculiar smell.

Purified water is suitable 20 ~ 40% by weight in alcoholic disinfectant containing green tea extract of the present name.

In addition, it may be prepared by further adding at least one selected from the group consisting of skin moisturizers, gelling agents, fragrances, antioxidants, and antimicrobial agents.

The skin moisturizer is used 0.1 to 5.0% by weight. The skin moisturizing agent may be at least one selected from the group consisting of glycerin, propylene glycol, isopropyl myristate, sorbitol, silicone derivatives, and aloe vera.

The gelling agent is used 0.1 to 5.0% by weight. The gelling agent may be at least one selected from the group consisting of collagen, gelatin, ethylcellulose, xanthan gum, guar gum, and carbomer.

The fragrance may be at least one selected from the group consisting of fruit flavors such as lemon, orange, pineapple, flower fragrance, such as lavender, rose, jasmine.

The antioxidant may be at least one selected from the group consisting of ascorbic acid, steine, glutathione, synthesized bile pigments, uric acid.

The antimicrobial agent may be at least one selected from the group consisting of chlorohexidine, 2.4.4'-triglo-2-2-hydroxydiphenylether, triclocarban, penicillin, tetracycline and bacitracin. .

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

The green tea extract-containing disinfectant disinfectant does not require water washing, so it is excellent in simplicity and convenience in hand washing, and isopropanol is used in the green tea-containing ethanol extract to enhance the antibacterial and bactericidal effect, thereby removing harmful microbial contamination. It is effective in skin protection and moisturizing by using skin moisturizer.

In addition, the green tea extract-containing disinfectant disinfectant according to the present invention contains alcohol, but when used as a disinfectant disinfectant may cause discomfort due to alcohol odor and odor, but the polyphenol component of green tea has the effect of removing the peculiar smell of alcohol without discomfort Anyone can use comfortably.

Hereinafter, preferred embodiments of the present invention will be described. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms.

Example 1 Preparation of Green Tea 20% Ethanol Extract

100 g of a 20% ethanol aqueous solution was added to 1 g of green tea dried leaves, followed by extraction at 25 ° C. for 2 hours, followed by filtration to prepare green tea 20% ethanol extract.

Example 2 Preparation of Green Tea 40% Ethanol Extract

In the same manner as in Example 1, 40% ethanol extract of green tea was prepared in 40% ethanol aqueous solution.

Example 3 Preparation of Green Tea 50% Ethanol Extract

In the same manner as in Example 1, 50% ethanol extract of green tea was prepared in a 50% ethanol aqueous solution.

Example 4 Preparation of 60% Ethanol Extract of Green Tea

In the same manner as in Example 1, 60% ethanol extract of green tea was prepared in an aqueous 60% ethanol solution.

Example 5 Preparation of Green Tea 80% Ethanol Extract

In the same manner as in Example 1, 80% ethanol extract of green tea was prepared in an 80% ethanol aqueous solution.

Example 6 Preparation of Green Tea 90% Ethanol Extract

In the same manner as in Example 1, 90% ethanol extract of green tea was prepared in 90% ethanol aqueous solution.

Example 7 Preparation of Green Tea 95% Ethanol Extract

In the same manner as in Example 1, 95% ethanol extract of green tea was prepared in an aqueous 95% ethanol solution.

Example 8 Preparation of 100% Ethanol Extract of Green Tea

In the same manner as in Example 1, 100% ethanol extract of green tea was prepared in 100% ethanol aqueous solution.

Example 9 Preparation of Green Tea Ethanol 1 Hour Extract

100 g of 60% ethanol aqueous solution was added to 1 g of green tea dried leaves, followed by extraction at 25 ° C. for 1 hour, followed by filtration to prepare green tea ethanol 1 hour extract.

Example 10 Preparation of Green Tea Ethanol 3 Hour Extract

In the same manner as in Example 9, but extracted for 3 hours to prepare a green tea ethanol extract 3 hours.

Example 11 Preparation of Green Tea Ethanol 5 Hour Extract

In the same manner as in Example 9, but extracted for 5 hours to prepare a green tea ethanol extract 5 hours.

Example 12 Preparation of Green Tea Ethanol 7-Hour Extract

In the same manner as in Example 9, but extracted for 7 hours to prepare a 7-hour extract of green tea ethanol.

Example 13 Preparation of 9-Hour Extract of Green Tea Ethanol

In the same manner as in Example 9, but extracted for 9 hours to prepare a green tea ethanol extract 9 hours.

Example 14 Preparation of Green Tea Ethanol 12 Hour Extract

In the same manner as in Example 9, but extracted for 12 hours to prepare a green tea ethanol 12 hours extract.

Example 15 Preparation of 15-Hour Extract of Green Tea Ethanol

In the same manner as in Example 9, but extracted for 15 hours to prepare a green tea ethanol extract 15 hours.

Example 16 Preparation of Green Tea Ethanol Extract-Containing Disinfectant

100 g of 60% ethanol aqueous solution was added to 1 g of green tea leaves and stirred at 25 ° C. for 5 hours, followed by filtration to extract 90% of 60% aqueous extract of 60% ethanol from green tea containing 0.5 g of total polyphenols, 7.5% by weight of ethanol, and 2.0% by weight of isopropanol. , 0.4% by weight of gelatin, 0.1% by weight of lemon flavor was stirred for 1 hour at room temperature to prepare a green tea ethanol extract-containing disinfectant.

Example 17 Preparation of Disinfectant Disinfectant Containing Green Tea Ethanol Extract Without Isopropanol

In the same manner as in Example 16, 9.5 wt% of ethanol was added without adding isopropanol to prepare a green tea ethanol extract-containing disinfectant.

Comparative Example 1 Preparation of Ethanol Disinfectant Disinfectant without Green Tea Extract

90% by weight of 60% ethanol aqueous solution, 2.0% by weight of isopropanol, 7.5% by weight of ethanol, 0.4% by weight of gelatin, 0.2% by weight of perfume was stirred for 30 minutes in a stirrer to prepare a disinfectant.

<Comparative Example 2> Preparation of ethanol sterilizer without isopropanol

In the same manner as in Comparative Example 1, ethanol-containing bactericidal disinfectant was prepared by adding 9.4% by weight of ethanol without adding isopropanol.

Test Example 1 Total Polyphenol Content According to Ethanol Ratio

Green Tea 20% Ethanol Extract (Example 1), Green Tea 40% Ethanol Extract (Example 2), Green Tea 50% Ethanol Extract (Example 3), Green Tea 60% Ethanol Extract (Example 4), Green Tea 80% Ethanol Extract ( Example 5), the total polyphenol content of the green tea 90% ethanol extract (Example 6), green tea 95% ethanol extract (Example 7), green tea 100% ethanol extract (Example 8) according to the ethanol ratio was evaluated .

Total polyphenol content was determined according to the Folin-Ciocalteu method. That is, 6 ml of distilled water and 0.5 ml of folin-Ciocalteu reagent were added and mixed with 0.1 ml of the sample. After 8 minutes, ₂ ml of 15% sodium carbonate (Na ₂ CO ₃ ) was added thereto, and the volume was adjusted to 10 ml. Then, after standing for 2 hours at room temperature, the absorbance was measured at 765 nm and quantified in terms of (+) catechin.

The results of comparative evaluation of total polyphenol content according to ethanol ratio are shown in Table 1 below.

Table 1. Total Polyphenol Content Results According to Ethanol Ratio

 Example  Ethanol Content (%)  Total Polyphenol Content (g / 100mL) Example 1 20 0.112 Example 2 40 0.124 Example 3 50 0.128 Example 4 60 0.130 Example 5 80 0.088 Example 6 90 0.062 Example 7 95 0.018 Example 8 100 0.006

Table 1 shows the total polyphenol content results of the green tea extract according to the ethanol ratio. The total polyphenol content of green tea 20, 40, 50 and 60% ethanol extract was 0.112g / 100mL, 0.124g / 100mL, 0.128g / 100mL and 0.130g / 100mL, and 80, 90, 95 and 100% ethanol The total polyphenol content of the aqueous solution was 0.088 g / 100 mL, 0.062 g / 100 mL, 0.018 g / 100 mL and 0.006 g / 100 mL.

Therefore, the green tea extract according to the present invention was found to have a significantly high total polyphenol content in 40 ~ 80% ethanol aqueous solution.

Test Example 2 Total Polyphenol Content According to Extraction Time

Green tea ethanol 1 hour extract (Example 9), green tea ethanol 3 hours extract (Example 10), green tea ethanol extract 5 hours (Example 11), green tea ethanol extract 7 hours (Example 12), green tea ethanol extract 9 hours ( Example 13), green tea ethanol 12 hours extract (Example 14), green tea ethanol 15 hours extract (Example 15) The total polyphenol content according to the extraction time was evaluated.

The total polyphenol content results according to extraction time are shown in Table 2.

Table 2. Results of Total Polyphenol Contents with Extraction Time

 Example  Extraction time (hr)  Total Polyphenol Content (g / 100mL) Example 9 One 0.115 Example 10 3 0.125 Example 11 5 0.130 Example 12 7 0.130 Example 13 9 0.130 Example 14 12 0.130 Example 15 15 0.130

Table 2 shows the total polyphenol content results according to the extraction time. The 1 hour green tea ethanol extract (Example 9) had a total polyphenol content of 0.115 g / 100 mL, and the 3 hour green tea ethanol extract (Example 10) had a total polyphenol content of 0.125 g / 100 mL. The extraction rate of total polyphenols was not increased any more than 0.130 g / 100mL in green tea ethanol extract at 7, 9, 12 and 15 hours.

Therefore, the green tea ethanol extract according to the present invention was confirmed that 3 to 5 hours is the proper extraction time.

Test Example 3 Bactericidal Effect of Bactericidal Disinfectant

The bactericidal effect of the disinfectant disinfectant prepared in Examples 16 to 17 and Comparative Examples 1 and 2 was evaluated. Example 16 (green tea extract-containing disinfectant), Example 17 (isopropanol-free green tea extract disinfectant), Comparative Example 1 (green tea extract-free ethanol disinfectant), Comparative Example 2 (green tea extract and isopropanol-free ethanol disinfectant) 1 minute after hand disinfection with a disinfectant disinfectant prepared in the) bacteria were collected from the hands with a sterile swab and smeared on a 2M petri film for general bacteria and incubated at 35 ℃ for 24 hours to measure the number of each bacterial community. The results of the comparative evaluation of the bactericidal effect of the bactericidal disinfectant are shown in Table 3.

<Evaluation Criteria>

none -

Less +

Normal ++

Plenty +++

Table 3. Results of bactericidal effect of green tea extract-containing fungicide

 Classification  Example 16  Example 17  Comparative Example 1  Comparative Example 2  Bacterial population  -  +  ++  ++

Table 3 shows the results of the bactericidal effect of the green tea disinfectant. Example 16 (green tea extract-containing disinfectant disinfectant) had no bacterial colonies, and Example 17 (isopropanol-free green tea extract-containing disinfectant disinfectant) had a small bacterial count, and Comparative Example 1 (green tea extract-free ethanol disinfectant) and Comparative Example 2 (green tea extract and isopropanol-free ethanol disinfectant) showed a normal bacterial population.

Therefore, the green tea extract-containing disinfectant of Example 16 according to the present invention was confirmed to have excellent hand disinfection sterilization effect.

<Test Example 4> Skin irritation test of disinfectant disinfectant

Comparative evaluation of the skin irritation degree of the disinfectant disinfectant prepared in Examples 16 to 17 and Comparative Examples 1-2. Example 16 (green tea extract-containing disinfectant), Example 17 (isopropanol-free green tea extract disinfectant), Comparative Example 1 (green tea extract-free ethanol disinfectant), Comparative Example 2 (green tea extract and isopropanol-free ethanol disinfectant) In order to determine the degree of skin irritation of the disinfectant disinfectant prepared in), 20 men and women were selected and used five times a day for 10 days, and then the skin irritation was measured. The skin irritation test results of the disinfectant disinfectant are shown in Table 4.

<Evaluation Criteria>

No irritation-

Slightly irritating +

Has irritation ++

Severe irritation +++

Table 4. Results of disinfectant skin irritation test

 Classification  Example 16  Example 17  Comparative Example 1  Comparative Example 2  Bacterial population  -  -  ++  ++

Table 4 shows the results of the disinfectant disinfectant skin irritation test evaluation. Example 16 (green tea extract-containing disinfectant) and Example 17 (isopropanol-free green tea extract disinfectant) had no skin irritation, Comparative Example 1 (green tea extract without ethanol disinfectant), Comparative Example 2 (with green tea extract) Isopropanol-free ethanol disinfectant) has been shown to be irritating.

Therefore, the green tea extract-containing bactericidal disinfectant of Examples 16 and 17 according to the present invention was confirmed to be safe to use without skin irritation.

<Test Example 5> fungicide disinfectant alcohol odor and odor test

The alcohol odor and off-flavor of Example 16 and Comparative Example 1 were evaluated.

Example 16 (green tea extract-containing disinfectant disinfectant), Comparative Example 1 (green tea extract-free ethanol disinfectant disinfectant) to determine the alcohol odor and odor of the disinfectant disinfectant prepared 20 men and women each compared to Example 16 As an antiseptic disinfectant of Example 1, the degree of alcohol odor after hand disinfection was checked. The bactericidal disinfectant alcohol and off-flavor comparative evaluation results are shown in Table 5.

<Evaluation Criteria>

No smell left 1

Little scent 2

Stinky 3

Smells Great 4

Table 5. Alcohol disinfection and odor test results

 Classification  Example 16  Comparative Example 1  Alcohol  1.2  4.5  Off-flavor  1.5  4.8

Table 5 shows the results of the bactericidal disinfectant alcohol odor and odor test evaluation. In Example 16 (green tea extract-containing disinfectant disinfectant), alcohol odor and odor were 1.2 and 1.5, and Comparative Example 1 (green tea extract ethanol disinfectant) was odor and 4.5 and 4.8.

Therefore, the green tea extract-containing sterilizing disinfectant of Example 16 according to the present invention has been found to be easy to use comfortably for everyone without discomfort because the polyphenol component contained in green tea has an effect of removing odors and odors peculiar to alcohol. .

1 is a flowchart sequentially illustrating a process of preparing the green tea ethanol extract-containing alcohol disinfectant disinfectant according to a preferred embodiment of the present invention.

Claims (8)

Skin moisturizing effect, containing 0.05-1.5% by weight of total polyphenol, 40-75% by weight of ethanol, 2-10% by weight of isopropanol, and 20-40% by weight of purified water Green ethanol extract-containing alcoholic antiseptic disinfectant, characterized in that the increase and decrease the alcohol odor. The method of claim 1, Green tea ethanol extract-containing alcoholic, characterized in that the addition of at least one selected from the group comprising a skin moisturizer, gelling agent, flavoring, antioxidant, antimicrobial agent to the green tea ethanol extract-containing alcoholic disinfectant Disinfectant.  The method of claim 2, The skin moisturizing agent is an alcohol disinfectant disinfectant containing green tea ethanol extract, characterized in that at least one selected from the group consisting of glycerin, propylene glycol, isopropyl myristate, sorbitol. The method of claim 2, The gelling agent is a green tea ethanol extract-containing alcoholic antiseptic disinfectant, characterized in that at least one selected from the group consisting of collagen, gelatin, ethyl cellulose, xanthan gum, guar gum, carbomer. The method of claim 2, The fragrance is a green tea ethanol extract-containing alcoholic antiseptic disinfectant, characterized in that at least one selected from the group consisting of fruit flavors, such as lemon, orange, pineapple, lavender, rose, jasmine. The method of claim 2, The antioxidants include plant extracts such as dibutylhydroxytoluene, butylhydroxyanisole, vitamin E and derivatives thereof, vitamin C and derivatives thereof, erythorbic acid and salts thereof, algae extract, hamamelis extract, tea extract and Green tea ethanol extract-containing alcohol disinfectant disinfectant, characterized in that at least one selected from the group containing gallate. The method of claim 2, Wherein the antimicrobial agent is at least one selected from the group consisting of chlorohexidine, 2.4.4'-triglolo-2'-hydroxydiphenylether, triclocarban, penicillin, tetracycline and bacitracin Green tea ethanol extract containing alcoholic antiseptic disinfectant. In the manufacturing method of alcoholic fungicide with green tea ethanol extract, Washing and drying the green tea leaves; The green tea leaves at 20-40 ℃ with 40-80% ethanol Extracting for 3 to 5 hours; Filtering the extract solution to concentrate the filtrate; And Mixing 0.05 to 1.5% by weight of total polyphenol, 40 to 75% by weight of ethanol, 2 to 10% by weight of isopropanol, and 20 to 40% by weight of purified water as a concentrate of the ethanol aqueous solution extract of green tea; Method for producing a green tea ethanol extract-containing alcohol disinfectant disinfectant comprising a.

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