WO2015152444A1 - Oral composition for inhibiting halitosis - Google Patents

Abstract

The present invention relates to an oral composition which has excellent effects in halitosis inhibition, dental caries inhibition and peridontal disease inhibition, the oral composition comprising natural medicinal ingredient extracts essentially comprising Scutellariae Radix extract and Nepetae Spica extract, and additionally comprising forsythia fruit extract, lonicer aflower extract and platycodi radix extract, etc., and the oral composition further comprising tocopheryl acetate and malic acid. The oral composition comprises 0.1 to 5.0 % by weight of a mixture of the natural medicinal ingredient extracts including Scutellariae Radix extract and Nepetae Spica extract, 0.01 to 1.0 % by weight of tocopheryl acetate, and 0.01 to 1.0 % by weight of malic acid.

Description

Oral composition for bad breath

The present invention relates to an oral composition for inhibiting bad breath, and in particular, it comprises a golden extract and a mold extract, and in addition to the natural medicinal extract including a duct extract, gold silver extract, gilyeong extract, etc. In addition, the present invention relates to an oral composition excellent in inhibiting bad breath, inhibiting dental caries and inhibiting periodontal disease, further including tocopherol acetate and malic acid.

Tooth decay is a disease in which carbohydrates in food are metabolized by microorganisms in the gingival and subgingival bacterial membranes, and the hard tissues of teeth are destroyed by the action of locally produced organic acids. One of the concerns. Tooth decay does not have a direct relationship to human life, but the chewing function of food deteriorates, so it directly or indirectly affects diseases of the digestive system. The prevalence of tooth decay is especially high in children, with 85% of children aged 5 or older experiencing or experiencing pain from tooth decay. This tooth decay is caused by Streptococcs mutans ( S. mutans ), among the microorganisms in the oral cavity. The mechanism of caries induced by the microorganisms is as follows. Streptococcus mutans is a Gram-positive anaerobic bacterium that resides in the dental plaque in the oral cavity. To destroy enamel of teeth (Kim Jong-bae et al., Public Oral Health Science, Advisor, pp. 68-84, 1991).

In most cases, bad breath causes more than 85% of the root causes of oral disease as a result of bacterial decay of host components and food residues in the oral cavity. Intraoral causes include tongue, periodontal disease, dirty oral hygiene, food residues, inadequate prostheses, unsanitary dentures, oral carcinoma, but the most important causes of bad breath are tongue and periodontal disease. Volatile sulfur compounds (VSC) are the main cause of bad breath: hydrogen sulfide (H ₂ S), methyl mercaptan (CH ₃ SH), dimethyl sulfide (CH ₃ SCH ₃ ) And hydrogen sulfide and methyl mercaptin account for about 90%. These VSCs are mainly caused by the decay activity of anaerobic bacteria in the oral cavity using gingival fissure, saliva, proteins in food residues (particularly those containing cysteine and methionine), peptides and amino acids as substrates. Is generated. Representative Gram-negative anaerobic bacteria include Porphyromonas gingivalis , Prevotella intermedia , Fusobacterium nucleatum , and large amounts of hydrogen sulfide and methylmercaptan. Is being reported. As a result, it has been reported that periodontal disease bacteria play an important role in inducing bad breath. Many studies have shown that volatile sulfur compounds have a detrimental effect on periodontal tissues. VSC's action on tissues increases mucosal permeability, collagen breakdown, changes in gingival fibroblasts and periodontal ligament cells, and wounds. The effects of delayed healing have been experimentally demonstrated (Kwon Jin-hee et al., The Korean Periodontological Society 2000).

MMP3, an intracellular matrix protein, is considered to be an important protease involved in tissue destruction and tissue reorganization in various diseases such as chronic inflammatory disease, rheumatoid arthritis, skin disease, and cancer, and polymorphonuclear leukocytes and fibroblasts associated with periodontal disease It is expressed in macrophages, epithelial cells, etc. and is known as the causative enzyme of periodontitis (Cho Jong-Hee et al 1999).

Periodontitis is an inflammatory disease that results in the destruction of tooth-supporting tissues such as gingiva, periodontal ligament, chalky and alveolar bone. Periodontitis is strongly associated with the presence of cytokines such as interleukin-1β (IL-1β). Physiologically, the activation of cyclooxygenases (COX) is closely related to periodontitis. In particular, COX-2 is induced by the production of cytokines and bacteria before inflammation (Kim, Sun-Woo et al. 2007).

On the other hand, the record of using various herbal medicines for the prevention or treatment of oral diseases has been seen from the past, and it has been said that various obtainable substances such as honey, rose oil, roasted salt, and herbal medicines have been used for the prevention or treatment of oral diseases. Although it is revealed through the records, there is still a need for the development of oral compositions excellent in inhibiting bad breath, inhibiting dental caries and periodontal disease.

The present invention has been conducted in order to solve the problems of the prior art, as a result, it is essential to include a golden extract and a mold extract, and, in addition to the natural medicinal extracts including yeongyo extract, gold silver extract and Gilyeong extract, etc. In addition, tocopherol acetate and malic acid were further included, and it was confirmed that it can provide the oral composition which is excellent in the bad breath suppression, the dental caries suppression, and the periodontal disease suppression effect, and came to complete this invention.

In particular, it is an object to provide an oral composition having a composition such that the pH is preferably maintained at 6 to 8, more preferably neutral such as saliva.

Oral composition for inhibiting bad breath according to the present invention, 0.1 to 5.0% by weight of the mixture of natural medicinal extracts, including golden extract and mold extract, 0.01 to 1.0% by weight of tocopherol acetate, 0.01 to 1.0% by weight of malic acid.

Preferably, the natural medicinal herb extract may further comprise 0.1 to 5.0% by weight of an extract selected from the group consisting of duct bridge extract, gilding extract, gilyeong extract and a mixture of two or more thereof.

Preferably, the oral composition for inhibiting bad breath may have a pH within the range of 6 to 8, more preferably may be neutral.

The oral composition for inhibiting bad breath may be a paste, and in particular, may be formulated with a toothpaste, mouthwash or mouthwash.

The oral composition for inhibiting bad breath, preferably, may be filled in a double container having a septum.

According to the present invention, essential extracts include golden extracts and mold extracts, and in addition, natural herbal extracts, including duct extracts, gold and silver extracts, and gil dynasty extracts, and the like, and further include tocopherol acetate and malic acid. Therefore, there is an effect of providing an oral composition excellent in inhibiting bad breath, inhibiting dental caries and periodontal disease.

1 is a graph showing the interleukin 1β gene expression inhibitory effect of the oral composition according to the present invention in comparison with the control and comparative examples.

Figure 2 is a graph showing the cyclooxygenase-2 (COX-2) gene expression inhibitory effect of the oral composition according to the present invention in comparison with the control and comparative examples.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Oral composition for inhibiting bad breath according to the present invention, characterized in that it comprises 0.1 to 5.0% by weight of the mixture of natural medicinal extracts, including golden extract and mold extract, 0.01 to 1.0% by weight of tocopherol acetate, 0.01 to 1.0% by weight of malic acid do.

The sterling silver extract, duct bridge extract, mold extract, gilyeong extracts used in the present invention may be extracted by conventional extraction methods well known in the art, preferably hot water extraction method or extracted by mixing ethanol or water and ethane Can be used.

The golden extract of the natural medicinal extract is an extract of the root of golden ( Scutellaria baicalensis GEORGE ), which is a perennial herbaceous plant belonging to the Lamiaceae. Gold is a herbaceous plant cultivated in various places and is widely used for medicinal, the active ingredient is known as Baikalein (Baicalein), Baikalin (Baicalin), wogonin (Wogonin), wogonoside (Wogonoside) and the like. The taste is bitter and weak. Efficacy is a fact that lowers (實 火), so the body has a lot of fever and hunger and thirst severely, if you improve the symptoms of conscious confusion. It is known that antipyretic effect is high from high fever due to cold to high fever of general infectious disease. Responds to the accumulation of heat in the lungs, which is a remarkable response to acute pneumonia, acute bronchitis, high fever and asthma is an excellent response. If gastrointestinal fever is severe and worsens digestive disorders, it is known to be effective in acute jaundice as well as symptoms of mouthache and stomatitis. Eliminates ocular hyperemia and pain caused by excessive heat build up in the body, high blood pressure, headache, nosebleeds, uterine bleeding, and is effective in skin boils. Such efficacy is known to be because cold medicine relieves symptoms because it lowers the fever. To quench the thirst caused by severe fever, and to relieve the symptoms of fever and pain in every joint joint. In pharmacological experiments, anti-inflammatory reactions, anti-pathogenic microorganisms, antipyretics, diuresis, lowering blood pressure, lowering blood sugar and hyperlipidemia, promoting bile secretion, and sedation were shown.

The hyunggae extract is an extract of the annual herb belonging to the family Lamiaceae called hyeonggae, hyeonggae is called purple, so the shape and color is similar to a purple tea, it is mainly used for medicinal rather than edible. In oriental medicine, Hyeonggae's outpost and the flowered ear are used as medicine for Hyeonggaesu. According to Dongbobom Herb, “The quality is warm, the taste is very bitter, and it is poisonous. Bad winds, red winds, lack of sensation all over the body, upper head hurts, tendons and bones are sweet and heal and blood flow, bleeding, healing Nagwa and Changyang. Plant in the field. When you are young, you can eat vegetables because they are fragrant and spicy. You can eat them raw or cooked. Also, if you make a tea over moon, your head and eyes will be cool. Cut the ears of flowers and seeds, dry them in the sun, and use them as medicine. The original name is Kaso because it smells and tastes like a perilla. ” According to Dongbogam, one of the most effective medicines for diaphragm is to treat dizziness and dizziness in front of eyes. It's good to eat decocted or powdered. To heal the swelling in the same amount of mold and gypsum (dalgun), each powder is powdered 8g at a time to eat ginger or green onions in water. Also known as Hyeonggae Mountain. Makes you sweat It also treats the blood breeze, which is eaten in water. Heals the tendons of hands and feet. Eat decoction in water. You can eat raw foods in full. The stroke causes the mouth and eyes to be crooked and swept away and to heal all the symptoms. Eat decoction in water. Squeeze it into the vinegar and paste it on the window. It is good to eat it by thickening it in water. When the head hurts to the upper limit, 40g of gyeonghyeong number is thickly eaten. Make yourself sweat and solve the ticket. Eat decoction in water. Heal what does not break. The drug is burned and the powder is eaten 8g at a time. ”

The present invention includes a natural medicinal herb extract comprising essentially the golden extract and hyeonghyeong extract, the natural medicinal extract is an amount within the range of 0.1 to 5.0% by weight based on the total weight of oral composition for inhibiting bad breath according to the present invention It is preferable for the bad breath inhibitory effect to be contained.

The oral composition for inhibiting bad breath according to the present invention further comprises tocopherol acetate and malic acid in addition to the above-mentioned natural herbal extracts.

The tocopherol acetate (tocopherol acetate) is a vitamin E acetate is different from the free vitamin E, the molecular formula is C ₃₁ H ₅₂ 0 ₃ , molecular weight 472.73 is a light yellow, viscous liquid. Acetate acetate is not affected by oxidizing agents such as air, light and ultraviolet light. Insoluble in water, soluble in acetone, chloroform and ether, slightly soluble in alcohol. Tocopherol is a vitamin E oil that not only acts as a natural antioxidant, but also has the effect of aging skin and cell regeneration, so it is mainly used in capsule form in skin. In particular, teeth have such a function, and it is known that the addition of an effective amount is effective in the prevention of gingivitis, periodontitis (alveolar pylori), and the prevention of periodontal and gingival diseases. The tocopherol acetate is preferably used in an amount within the range of 0.01 to 1.0% by weight based on the total weight of oral composition for inhibiting bad breath according to the present invention.

The malic acid (Malic Acid) is also referred to as umseongumum otherwise. It is a colorless acicular crystal with the chemical formula C ₄ H ₆ O ₅ , one asymmetric carbon atom, and D-, L-, and DL-3 optical isomers. Left-rotating L-malic acid is found in natural fruits and vegetables, such as apples and grapes. Deliquescent, molecular weight 134.09, specific gravity 1.595, melting point 100 ℃, boiling point 140 ℃. Compared to citric acid, citric acid, which has the same acidity, is not only cheaper in price, but also elegant and refined high-quality acidity and long-lasting acidity make people's appetite more pleasant and sour. It is also an acidic agent necessary for carbohydrate metabolism in humans, and especially in the oral cavity, it is an acidic acid, which reduces and inhibits bad breath and acts as a pH regulator. The malic acid is preferably used in an amount within the range of 0.01 to 1.0% by weight based on the total weight of the oral composition for inhibiting bad breath according to the present invention.

In contrast, the natural medicinal herb extract ranges from 0.1 to 5.0% by weight, based on the total weight of the oral composition for inhibiting bad breath according to the present invention, the extract selected from the group consisting of duct bridge extract, gold silver extract, gilyeong extract and a mixture of two or more thereof It is especially preferable for the bad breath inhibitory effect to contain in the quantity within the below.

The Yeongyo extract is an extract of the fruit of the Forsythia forsythia and the same plant belonging to the ash family called Yeongyo. Yeon-gyo's temper is bitter and cold, and the ear canal is known to act on the heart, menopause and liver. In addition, it is known to have the effects of sweating, clearing, drainage, antipyretic, antibacterial, anti-inflammatory, fever, headache, dry mouth, seawater, sore throat, boils, vomiting, vasodilation and blood flow improvement. In addition, diuretic effect (to make urine well), cardiac diuresis (to strengthen the heart and urine well), clearing detoxification (cools the heat, removes toxins), small squirt (to cool the boil and loosen the masses) It is known that there is an effect.

The sterling silver extract is a semi-heterophyllum vine plant of the genus Rosaceae, and is an extract of the plant of the cedar. From ancient times, when it is stuffy and thirsty, treatment of boils, skin is loose, organ inflammation and pus discharge, skin necrosis by dysentery or heat poisoning, mumps or conjunctivitis, and tonsils, sore throat, bronchitis It was mainly used for the treatment of inflammation such as cystitis. According to classical medicine, the nature of honeysuckle is cold, sweet and bitter, acts on the heart and menopause, lowers the heat, releases the poison, improves the meridians, has excellent effects on various inflammatory diseases, It is said that heals boils and boils.

The Giltyeong extract is an extract of Giltyeong, ie Bellflower. Bellflower is native to Korea and is common enough to be recognized as one of the three color herbs that are edible a lot of bellflowers, bellflowers, etc. Old bellflower is better than wild ginseng. ” Indeed, the excellent alkaline food bellflower is rich in dietary fiber as well as minerals such as calcium, iron and potassium, as well as beneficial amino acids such as vitamins B1, B2, vitamin C and tyrosine and tryptophan. It is said that there are 278 records about the efficacy of bellflower. Bellflower is a perennial plant belonging to the lantern family. In the name of the herbal medicine, Gilgyeong, Bangdo, Paekak, Goryeo, Kogyeong, also called Yeo. The root is mainly used as a medicine, which is taken in the fall, peeled and dried in the sun, and then the stem is removed and used. The root contains saponins, Platicodin and Platicodigenin. The bellflower is commonly used for cough and phlegm. In particular, the bellflower contains about 2g per 100g of bitter saponin, which is known as the main ingredient of ginseng, and triterpenoid-based saponins show efficacy in respiratory diseases. It is known to increase mucus secretion of the respiratory mucosa and to cut phlegm. Bellflower juice, which is a mixture of bellflower and pears, is widely used as a winter preventive medicine. The main ingredient of Yonggaksan, famous for cough and phlegm, is bellflower. Especially in oriental medicine, it is known to be effective as drainage, expectorant, tonsillitis, oily, cough, purulent boil, expectorant of asthma and pulmonary tuberculosis, and pleurisy. Bellflower is known to be beneficial for people with high blood pressure and diabetes who weigh a lot because it lowers blood pressure and blood sugar. Bellflower is known to help people with hyperlipidemia because it increases the secretion of steroids and galic acid in the body, leading to a decrease in cholesterol in the body. Recently, tumor suppressors have been isolated from bellflower, and antimutagenic effects have been identified. It was also found that when the bellflower extract was extracted with ethyl acetate, hepatic cancer cells reacted with a strong antioxidant effect, protecting the cells from cancer by oxidative stress.

Oral composition for inhibiting bad breath according to the present invention preferably further comprises citric acid in an amount within the range of 0.01 to 1.0% by weight in addition to the malic acid.

The oral composition for inhibiting bad breath is preferably used in an amount such that the pH is within the range of 6 to 8, preferably neutral.

Oral composition containing the extract or compound of the present invention in the present invention can be used to prepare a paste and liquid, preferably, the bad breath control oral composition may be a paste, but is not limited thereto.

The oral composition of the present invention may be formulated as a toothpaste, mouthwash or mouthwash. In particular, the toothpaste may comprise a composition selected from the group consisting of abrasives, caking agents, moisturizers, active ingredients, foaming agents, sweeteners, flavoring agents as the formulation ingredients, the formulation ingredients for formulation to these toothpastes are It can be understood as known.

Examples of the abrasive include aluminum hydroxide, silicic anhydride, aluminum silicate, dicalcium phosphate, dihydrate and anhydride, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, magnesium triphosphate, magnesium carbonate, and sulfuric acid. Calcium, polymethyl methacrylate, and other synthetic resins may be used in combination of one or two or more of them in an amount of about 20 to 90% by weight of the total composition (in the case of paste toothpaste, it may be within the range of 20 to 60% by weight). Can be).

Examples of the binder include carrageenan, various incremental cellulose derivatives, xanthan gum, gums such as tragacanth gum, polyvinyl alcohol, sodium polyacrylate, polyacrylic acid / maleic acid copolymer, and carboxyvinyl polymer. In organic binders, such as a polymer derivative, and inorganic binders, such as a silica and a laponite, 1 type, or 2 or more types can be mix | blended and used in the quantity within the range of about 0.3 to 5 weight% of the whole composition.

In the pasty and liquid oral composition, the moisturizing agent may include one or two or more of about 10 to 70% of sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol, etc. It can be used in combination.

In addition, sweeteners such as sodium saccharin, stevioside, aspartame, D-Xylose, and the like may be used in combination in amounts ranging from 0 to 1%, preferably 0.01 to 0.5% by weight of the total composition. .

In addition, as the surfactant used in the oral composition of the present invention as a foaming component, anionic surfactants, nonionic surfactants, zwitterionic surfactants and / or Pluron-based and poloxamer-based surfactants may be used. . Here, the anionic surfactants include sodium lauryl sulfate, sodium alkyl sulfate such as myristyl sulfate, sodium N-lauroyl sarcosinate, sodium N-miristyl sarcosinate, and the like. Sodium N-acylsarcosinate (Sodium N-acylsarcosinate) may be used, and non-ionic surfactants, sugar fatty acid esters such as sugar fatty acid esters, maltose fatty acid esters, lactose fatty acid esters, polyoxyethylene Cured castor oil, polyoxyethylene higher alcohol ether, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene polypropylene fatty acid ester and the like can be used. Zwitterionic surfactants include N-alkyl diaminoethylglycine, such as N-lauryl di aminoethylglycine, N-myristyl diaminoethylglycine, N-alkyl-N-carboxy methyl ammonium betaine, 2-alkyl-1 -Hydroxy ethyl imidazoline betaine sodium, etc. can be used. And pluron-based and poloxamer-based surfactants may be used. In this case, anionic surfactants are mainly used as foaming components, and sodium alkyl sulfates such as sodium lauryl sulfate are most widely used. In addition, these surfactant is used individually by 1 type or in mixture of 2 or more types, The compounding quantity is used in the range of 0.01-0.5 weight%, More preferably, 0.05-0.3 weight% of the whole composition normally.

In addition to the above, oral compositions of the present invention include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic and natural flavors, colorants and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the oral compositions of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0 to 20 parts by weight per 100 parts by weight of the composition of the present invention.

The mouthwash or mouthwash may also include suitable formulation ingredients, and formulating ingredients for formulation into these mouthwashes or mouthwashes will be understood by those skilled in the art, for example, carriers. It may include water to maintain a liquid at room temperature, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, etc., and may further include other colorants, flavors, flavors, sweeteners and the like.

The oral composition for inhibiting bad breath may be filled in a double container having a septum. When filled in a double container having such a diaphragm, it may be desirable to satisfy the preference of the consumer to have the same composition on both sides based on the diaphragm of the double container, but filled with different colors.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Example 1 described in Table 1 below is an oral composition comprising a natural medicinal herb extract and tocopherol acetate and malic acid as essential, including a golden extract and a mold extract according to the present invention, Example 2 is a natural medicinal herbs in addition to Example 1 It is the same oral composition as in Example 1, except that the extract further comprises an equivalent amount of Yeongyo extract, Geumgumhwa extract and Gilgyeong extract. On the other hand, the oral composition of Comparative Example 1 is an oral composition, which is configured in the same manner as in Example 1, except that in Example 1 it does not contain the golden extract, mold extract, tocopherol acetate and malic acid.

After precisely weighing and mixing the composition and content of the following tables, it was prepared as a toothpaste by a general toothpaste manufacturing method.

Experimental Example 1 Antibacterial Activity Test of Streptococcus mutans

The oral compositions of Comparative Example 1 and Examples 1 and 2 were formulated with toothpaste, and 2 g of the oral composition formulated with toothpaste was suspended and diluted in 9 g of sterile phosphate buffer solution.

Table 1

Raw material name	Comparative Example 1	Example 1	Example 2
Amorphous sorbitol	30	30	30
Polyethylene Glycol 300	2	2	2
Carboxymethyl Cellulose	One	One	One
Tocopherol Acetate	-	0.1	0.1
Zaccarin Sodium	0.22	0.22	0.22
Apple and mountain	-	0.1	0.1
Calcium Phosphate Dichloride	0.1	0.1	0.1
Sedimentation Silica	30	30	30
Sedimentation silica (for increasing)	3	3	3
Sodium Lauryl Sulfate	1.55	1.55	1.55
Sodium fluoride	0.22	0.22	0.22
Golden extract	-	0.5	0.5
Mold extract	-	0.5	0.5
Duct extract	-	-	0.5
Gold Coin Extract	-	-	0.5
Gilt Extract	-	-	0.5
Yellow No. 4 (FDC ＃ 5)	0.002	0.002	0.002
Green No. 3 (FDC ＃ 5)	0.0005	0.0005	0.0005
Toothpaste Flavor	1.05	1.05	1.05
Titanium dioxide	0.1	0.1	0.1
Sodium monophosphate	0.1	0.1	0.1
Trisodium Phosphate	0.05	0.05	0.05
Purified water	30.6075	29.4075	27.9075

The diluted suspension was serially diluted 2-fold with BHI (Brain heart infusion) medium and injected into 1 ml of 48 well plates. In addition, one Streptococcus mutans (ATCC 25175) colony was suspended in 20 ml BHI medium in an agar medium and additionally injected into the diluted 48-well plate and incubated at 37 ° C. for 24 hours. Was measured by MIC (minimal inhibition concentration), and the results are shown in Table 2 below.

TABLE 2

Kinds	Comparative Example 1	Example 1	Example 2
Mg / ml	12.5	6.25	6.25

As a result, according to the present invention, natural herbal extracts including golden extract and mold extract and oral composition comprising tocopherol acetate and malic acid (Example 1) and Yeongyo extract, Geummi extract and Gilpyeong extract in addition to Example 1 were further added. It was confirmed that the oral composition (Example 2) containing the superior antimicrobial activity compared to Comparative Example 1 otherwise.

Experimental Example 2 Antimicrobial Activity Test in Anaerobic Microorganisms

As in Experiment 1, 2g of the oral composition of Comparative Example and Examples was diluted and suspended in 9g of sterile phosphate buffer, and the experiment was carried out as follows. MIC method was carried out in the same manner as in Experiment 1. However, because anaerobic microorganisms do not grow in the presence of oxygen, they were cultured in an anaerobic incubator (Sheldon Manufactoring Bac 2-2E) supplied with 5% hydrogen (H ₂ ) and 95% nitrogen (N ₂ ). The results are shown in Table 3 below.

TABLE 3

Strain (mg / ml)	Comparative Example 1	Example 1	Example 2
Popuromonas Serious Bally ATCC 33277	12.50	6.50	6.50
Prebotella Intermediate ATCC 25611	6.50	6.50	6.50
Husobacterium nucleatumATCC 25586	6.50	6.50	6.50

As a result, according to the present invention, oral compositions containing herbal prescriptions (Examples 1 and 2) were shown to be similar to Comparative Example 1 in antibacterial activity, but they also exhibited antibacterial activity against Popuromonas gingivavalis which is a periodontitis-inducing bacterium. It is also expected to be excellent in inhibiting periodontal inflammation.

Experimental Example 3 Evaluation of Activity Inhibitory Activity of MMP-3

In the oral compositions of Comparative Example 1 and Examples 1 and 2, as shown in Table 4, using the formulation except for sodium fluoride, sodium lauryl sulfate, flavor, pigment, etc. (Comparative Examples 2 and 3 and 4) The inhibitory activity of MMP-3 was evaluated.

Table 4

Raw material name	Comparative Example 2	Example 3	Example 4
Amorphous sorbitol	30	30	30
Polyethylene Glycol 300	2	2	2
Carboxymethyl Cellulose	One	One	One
Tocopherol Acetate	-	0.1	0.1
Zaccarin Sodium	0.22	0.22	0.22
Apple and mountain	-	0.1	0.1
Calcium Phosphate Dichloride	0.1	0.1	0.1
Golden extract		0.5	0.5
Mold extract		0.5	0.5
Duct extract		-	0.5
Gold Coin Extract		-	0.5
Gilt Extract		-	0.5
Titanium dioxide	0.1	0.1	0.1
Sodium monophosphate	0.1	0.1	0.1
Trisodium Phosphate	0.05	0.05	0.05
Purified water	66.43	65.23	63.73

Fibroblasts were cultured to see the inhibitory activity of MMP-3 and treated with the oral composition. Human gum fibroblasts (Human Gingvial Fibroblast) were collected from healthy tissues of humans without a history of periodontitis and used for the first culture. Fetal bovine serum (fetal bovine serum) (GibcoBRL, USA) and 1% antibiotics / antimycotics (100 U / ml penicillin, 25 µg / ml amphotericin) Cultured in a 37 ° C., 5% carbon dioxide (CO ₂ ) incubator using Dulbecco's modified Eagle's medium (DMEM) medium containing D) and 100 μg / ml streptomycin. Gum fibroblasts from humans cultured in cultured with 1% FBS were incubated for 24 hours, followed by sodium monofluorophosphate, sodium lauryl sulfate, paraoxybenzoic acid and mint B70097, which may cause cell toxicity. The other oral compositions were treated with phosphate buffer at a concentration of 200 ppm and 500 ppm for 30 minutes, followed by addition of interleukin-1β (100 ng / ml) and incubated for 48 hours (MMP-3). Supernatant only by centrifugation during MMP-3 activity was measured using an ELISA kit (Amersham Bioscience, UK), and the results are shown in Table 7.

Table 5

MMP-3 Activity (ng / ml)	No treatment group	IL-1β	Comparative Example 2+ IL-1β	Example 3+ IL-1β	Example 4+ IL-1β
200 ppm	4.25	17.50	16.36	9.25	8.95
500 ppm	4.67	18.10	14.56	4.76	4.50

According to the present invention, based on the composition of the herbal prescription, gyeonghyang extract, including the gyeonghyang extract (Example 3) is shown to be more effective than the comparative example 2, Yeongyo extract, gyeonghyang extract as the main inhibitor component It seems to work. In addition, the composition of herbal medicine containing Yeongyo extract, hyunggwa extract, gilding extract, gilyeong extract (Example 4) is a little better MMP-3 inhibitory effect than Example 3, so the gilge extract, gilyeong extract is yeonkyo extract, hyunggae The extract and synergistic effect are considered to be indicative.

Experimental Example 4 Anti-inflammatory Indices Interleukin-1β and Cyclooxygenase-2 (cox-2) Expression Inhibition Test (RT-PCR)

Dispense 1 × 10 ⁶ Raw264.7 cell lines into 100 mm Petri dishes and remove the oral composition except sodium monofluorophosphate, sodium lauryl sulfate, paraoxybenzoic acid methyl, and mint B70097, which may cause cellular toxicity in the formulation. Treatment with phosphate buffer at 200ppm, 500ppm concentration, induce inflammation with LPS, incubate for 24 hours, remove the medium, remove the cells from the culture vessel, and use the ribonucleic acid extract solution (Invitrogen, CA, USA) Was homogenized. After 5 minutes, the cells were collected and transferred to a centrifuge tube. 200 μl of chloroform was added and mixed thoroughly for 15 seconds. The mixture was left for 3 minutes and centrifuged at 14000 rpm for 15 minutes. The supernatant containing ribonucleic acid was transferred to a new tube and mixed with 500 μl of isopropyl alcohol. After 10 minutes, the supernatant was discarded again by centrifugation, 1 ml of 75% ethanol was added to the precipitate, and centrifuged at 10000 rpm for 5 minutes, the supernatant was discarded, and the precipitated ribonucleic acid was dried at room temperature for 20 minutes. The dried ribonucleic acid was suspended in distilled water treated with DEPC (Diethylpyrocarbonate, Sigma). After quantification of ribonucleic acid, complementary nucleic acid (cDNA) was synthesized using RT-PreMix (AccuPower RT PreMix, Bioneer). Synthesized cDNA was used as a template to mix interleukin-1β and cyclooxygenase-2 primers, and the expression level of ribonucleic acid was confirmed using PCR Premix (AccuPower PCR PreMix, Bioneer). COX-2 primers were mixed with the synthesized cDNA template, and the expression level of ribonucleic acid was confirmed using PCR Premix (AccuPower PCR PreMix, Bioneer). Quantitative analysis was performed using a densitometer (Image Gauge V 3.46, Koshin Graphic Systems, Fuji Photo Film Co., Japan).

As a result, as shown in Figures 1 and 2, as a result of examining the relative expression inhibition rate of the LPS-treated group, according to the present invention, the composition comprising a herbal prescription (combination extract, formulation containing a hyunggae extract) (Examples 3 and 4) In Comparative Example 2 it was confirmed that the interleukin-1β and cyclooxygenase-2 gene expression more effectively suppressed. In particular, the expression inhibition rate of cyclooxygenase-2 was confirmed to be significantly reduced by 50% or more in Example 3, 4 500ppm. In this result, it is judged that the suppression rate of the oral composition (Examples 3 and 4) containing an herbal prescription according to this invention is excellent. From this, it was confirmed that the herbal composition prescribed according to the present invention is very effective in inhibiting periodontal anti-inflammatory.

All oral compositions of Example 1 of the present invention, including herbal prescription, effectively inhibit the growth of dental caries, bad breath-causing and periodontitis-causing bacteria, MMP-3 directly involved in periodontal tissue destruction, interleukin-1β and cycloox It was confirmed to effectively inhibit the gene genease-2 expression.

The present invention can be used in the industry of manufacturing hygiene products, especially oral compositions such as toothpaste or mouthwashes.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (7)

1. Oral composition for inhibiting bad breath, characterized in that it comprises 0.1 to 5.0% by weight of the mixture of natural medicinal extracts, including golden extract and mold extract, 0.01 to 1.0% by weight of tocopherol acetate, 0.01 to 1.0% by weight of malic acid.
2. The method of claim 1,

   Oral composition for inhibiting bad breath, characterized in that the natural medicinal extract further comprises 0.1 to 5.0% by weight of the extract selected from the group consisting of Yeongyo extract, Geumhwa extract, Gilgeung extract and a mixture of two or more thereof.
3. The method of claim 1,

   Oral composition for inhibiting bad breath, characterized in that within the range of pH within the range of 6 to 8.
4. The method of claim 3, wherein

   Oral composition for inhibiting bad breath, characterized in that the oral composition for inhibiting bad breath.
5. The method of claim 1,

   The bad breath suppression oral composition is characterized in that the paste is a bad breath suppressor.
6. The method of claim 1,

   Oral composition for inhibiting bad breath, characterized in that formulated with toothpaste, mouthwash or mouthwash.
7. The method of claim 1,

   Oral composition for inhibiting bad breath is characterized in that the oral cavity is filled in a double container with a diaphragm.

Images