KR102328978B1 - An oral composition for improvement of periodontal disease comprising natural complex extracts - Google Patents

Abstract

The present invention relates to an oral composition for alleviating gingivitis or periodontitis. Provided is an oral composition comprising a Coptis japonica extract and a Sophora flavescens extract as active ingredients.

Description

Oral composition for periodontal disease improvement containing a natural complex extract

The present invention relates to an oral composition for improving periodontal disease comprising a natural complex extract as an active ingredient.

As the concept of dental health is changed to the concept of social life, interest in oral health is increasing, and interest in bad breath, which is an obstacle in interpersonal relationships as well as prevention and treatment of oral diseases, is also increasing.

Teeth are made up of enamel, cementum and dentin. The outside of the tooth exposed to the outside is covered with enamel, the outside of the tooth root located in the alveolar bone is covered with cementum, and dentin exists inside. In dentin, microtubules called dentinal tubules are distributed throughout the dentin.

Periodontal disease is known to cause various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue, bad breath and sore throat, and is known as a major factor in causing tooth loss.

Periodontal disease is caused by various bacteria living in the oral cavity.

Harmful oral bacteria feed on residues such as food and proliferate in the acquired film to form plaque, and the plaque combines with metal ions such as calcium and magnesium in saliva or blood to form calcified calculus.

Plaque and tartar cause periodontal diseases such as gingivitis and periodontitis, and further damage teeth.

As a method of inhibiting the proliferation of oral pathogens that inhabit the dental plaque and cause various diseases, various types of antibacterial agents including antibiotics that have a sterilizing action on the bacteria have been developed as therapeutic agents.

However, since antibiotics may cause the appearance of resistant bacteria and mycosis in the oral cavity along with side effects in vivo, long-term use is not recommended and can be used only as a therapeutic agent.

Under this background, the present inventors have excellent biosafety, and as a result of earnest efforts to find a natural product that can inhibit the activity of oral disease-causing bacteria, the complex extract containing H. By confirming that it can be usefully used for the improvement of oral diseases, the present invention has been completed.

The present invention is to solve the problems of the prior art described above, and an object of the present invention is to provide an oral composition having excellent biosafety and excellent inhibitory activity against harmful bacteria in the oral cavity.

According to one aspect of the present invention, there is provided an oral composition for improving gingivitis or periodontitis, comprising an extract of Hwangryeon lily and ginseng extract as active ingredients.

In one embodiment, the oral composition may further include a licorice extract.

In one embodiment, the oral composition may further include a green tea extract.

In one embodiment, the oral composition may further include an anti-inflammatory use.

In one embodiment, the oral composition may further include an antibacterial use for S. Mutans or P. Gingivitis.

In one embodiment, the oral composition may further include a use for preventing tartar or bad breath.

In one embodiment, the extract may be an ethanol extract at a concentration of 60 to 80% (v/v).

In one embodiment, the oral composition may be formulated with one or more selected from the group consisting of mouthwash, toothpaste, oral spray, oral ointment, oral patch, and gum.

According to the present invention, since the oral composition removes the dental plaque through excellent antibacterial activity, it is possible to suppress the formation of tartar.

The oral composition can effectively suppress bad breath.

The oral composition can effectively suppress inflammation in the oral cavity.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like.

In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. Any numerical limitation given throughout this specification shall include all numerical ranges within the broader numerical range, as if the narrower numerical limitation were expressly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

One aspect of the present invention provides an oral composition for improving gingivitis or periodontitis, comprising an extract of H.

The term “oral composition” refers to a product that is retained in the oral cavity for a period of time sufficient to contact substantially all of the tooth surface or oral mucosal tissue for the purpose of oral activation, rather than intentionally swallowing for the purpose of systemic administration of a therapeutic agent.

The oral composition may be conventionally formulated as a mouthwash, but is not limited thereto, and may be implemented as various types of oral products such as toothpaste, oral spray, oral ointment, oral patch and gum. have.

Since the oral composition contains the extract of H. rhododendron and ginseng extract at the same time, it is possible to effectively prevent and improve periodontal disease that may occur in the oral cavity by the synergistic activity of active ingredients.

The "periodontal disease" is a disease that appears in the tissues surrounding the teeth, such as the gingiva, periodontal ligament, and alveolar bone surrounding the teeth. may be caused by

The periodontal disease may be classified into gingivitis and periodontitis according to the severity of the disease.

The “gingivitis” is a relatively light and rapid type of periodontal disease, and refers to a form limited only to the gums, that is, soft tissues, and the “periodontitis” may refer to a case in which inflammation has progressed to the gums and around the gums.

The “improvement” refers to any action that at least reduces the degree of lesion in the oral cavity by using the composition.

The " Coptis Rhizome " is a herbaceous perennial plant of the Ranunculaceae family. CY Cheng et Hsiao) or Unryeon (雲連, Coptis teeta Wallich) refers to the rootstock removed.

The high ginseng ( Sophora flavescens ; SF) is a perennial plant belonging to the legume family (Fabaceae family), and its roots have been used as a traditional treatment for fever, dysentery, pain, and the like. It has been reported that the high ginseng has an anti-infective effect, an anti-rotavirus effect, and a wound healing effect.

The "extract" means that the active ingredient contained in the extraction material is separated by contacting the solvent and the extraction material under specific conditions.

The complex extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, and the alcohol is 60 to It may be ethanol at a concentration of 80% (v/v), preferably ethanol at a concentration of 70% (v/v).

The extraction ratio of the active ingredients contained in the raw material may be different depending on the polarity of the solvent, and the ethanol has excellent selectivity in extracting physiologically active substances from natural raw materials, so the optimal periodontal disease improvement effect is realized by the ethanol extraction can be

In particular, since water and ethanol have different polarities, the active ingredients extracted according to each polarity may vary, and the concentration of the ethanol may be appropriately controlled so that an optimal periodontal disease improvement effect can be realized.

If the concentration of the ethanol is more than 80% (v / v), an appropriate yield may not be realized, and if it is less than 60% (v / v), the active ingredient having physiological activities such as antibacterial and anti-inflammatory activity may not be sufficiently extracted. .

The complex extract is obtained by washing each raw material with water, then drying and pulverizing it, and using a solvent with a volume equivalent to 8 to 12 times the weight of the raw material, reflux circulation extraction, pressure extraction, ultrasonic extraction, etc. for about 1 hour to 24 hours. It can be prepared by extraction and filtration. In addition, the extract may be obtained in a powder state by an additional process such as distillation under reduced pressure or freeze-drying.

The extract may also include an extract that has undergone a conventional purification process. For example, the extract is separated using an ultrafiltration membrane having a constant molecular weight cut-off value, and various purification methods are additionally performed such as separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity). It may include a fraction obtained through

In addition, since the extract is sufficient as long as it contains the active ingredient contained in the natural raw material, it can be obtained not only in the form of an extract obtained using a predetermined solvent, but also in the form of a crushed product or a pulverized product of the natural raw material.

The oral composition may further include a licorice extract.

The "licorice ( Glyccyrrhizae Radix , licorice roots)" is a medicinal plant belonging to the legume family, and can be used by using the roots and rhizomes as they are, or by removing the bark.

The licorice has been used for the purpose of relieving, analgesic, detoxification, etc. from ancient times (Germany, Herbal Medicine Revision Committee, Gongminsa, 2010), and its extract is known to have effects such as brain cell protection, anticancer, and anti-inflammatory.

The licorice extract not only has a chelating action, but also effectively inhibits the formation of dental plaque through antibacterial action, so it is possible to minimize the formation of tartar.

In addition, the licorice extract can be further enhanced oral activity when co-acting with the yellow lily and ginseng extract.

The oral composition may further include a green tea extract.

The green tea leaves contain a large amount of catechins to have excellent antibacterial and anticancer effects, and contain abundant amounts of vitamin C, vitamin E, beta-carotene, dietary fiber, minerals and chlorophyll.

It is reported that the green tea leaves have various effects such as antioxidant effect, skin elasticity improvement effect, anti-aging effect, heavy metal removal effect, anti-allergic effect, inflammation treatment effect, and immunity enhancement.

The green tea extract inhibits the growth of bacteria in the oral cavity through an antibacterial action, inhibits the formation of dental plaque, and simultaneously acts with the yellow liana and ginseng extract to further enhance oral activity.

On the other hand, the oral composition may include 300 parts by weight of ginseng extract, 300 parts by weight of ginseng extract, 200 parts by weight of licorice extract, and 200 parts by weight of green tea extract.

Since the extracts each have different active ingredients, and the activity according to the mixing ratio may also vary, the optimal oral activity may be realized by varying the mixing ratio of the extract.

The oral composition may further comprise an anti-inflammatory use.

The “inflammation” refers to a phenomenon that appears for a series of defensive purposes to minimize the reaction and restore the damaged area to its original state when a cell or tissue is damaged by any cause. Functional disorders that cause a reaction and consequently pain, swelling, redness, fever, etc. can be collectively referred to.

The “anti-inflammatory” refers to the action of inhibiting inflammation, and the regulation of the inflammatory response is known to be very complex, which reduces the enhancement and damage of the repair system in vivo.

If the inflammatory response continues due to repeated tissue damage or regeneration, ROS and RNS are overproduced in inflammation-related cells, and permanent gene modification may be caused.

The oral composition may further include an antibacterial use for S. Mutans or P. Gingivitis.

The “ S. Mutans” is a Gram-positive bacteria known as dental caries, which is a bacteria that pre-digests food in the mouth or ferments it when it comes in contact with sugars to produce acid and produce sticky substances. By producing a biofilm with the strain, it can promote the adhesion of these bacteria to the teeth.

At this time, the acid (acid) made from the metabolic product of the bacteria attacks the tooth surface, makes a hole in the enamel, and is known to cause tooth decay. have.

The “ P. Gingivitis ” is a kind of Bacteroide-like bacteria that is Gram-negative and anaerobic. The bacteria gingivalis is found in the oral cavity where periodontal disease has occurred, and in addition, it is also found in the upper gastrointestinal tract, respiratory tract and colon.

Since the bacteria gingivalis is associated with periodontal disease in the oral cavity, it is possible to suppress the onset of periodontal disease by inhibiting the bacteria gingivalis.

The oral composition may further include an anti-tartar or bad breath suppression use.

The "dental calculus" is a dental plaque that is firmly attached to the tooth surface, and calcium (Ca) derived from saliva and gingival sulcus (space between teeth and gums) fluid in the dental plaque. ), phosphorus (P), etc. may be deposited and formed.

The oral composition can effectively prevent the formation of tartar through the antibacterial activity against the dental plaque while ionic bonding with the inorganic substances contained in the food debris or foreign substances in the mouth during mouth washing.

The “bad breath” is a bad odor emitted from the mouth when breathing or talking, and may occur anywhere in the passages from the lungs to the outside of the mouth, that is, the lungs, bronchial tubes, throat, nasal passages, and oral cavity.

Volatile sulfur compounds (VSC) generated by bacterial decay by gram-negative anaerobic bacteria may be the cause of the occurrence of bad breath in the oral cavity.

Halitosis caused by the metabolism of bacteria is caused by anaerobic bacteria decomposing amino acids containing sulfur, which are volatile sulfur compounds such as hydrogen sulfide, methyl mercaptan, dimethyl sulfide, n-tetradicanol (n -tetradecanol), phenol, diphenylamine and pyridine.

Non-oral causes of the occurrence of bad breath may include respiratory diseases, neurological gastrointestinal diseases, other various diseases, and use of specific drugs. may occur.

The oral composition can effectively suppress bad breath through the alleviating effect on malodor itself, while at the same time removing the source of bad breath through antibacterial and anti-inflammatory activity.

The oral composition may further include an astringent.

The astringent acts locally on the skin or mucous membranes to coagulate proteins to remove inflammation and to create a film to protect, for example, sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium sulfate, potassium sulfate, sodium hydrogen carbonate, sodium carbonate, At least one may be selected from the group consisting of potassium monobasic, dipotassium phosphate, tripotassium phosphate, monobasic sodium phosphate, dibasic sodium phosphate, trisodium phosphate, and sodium saccharin, but is not limited thereto.

The sodium chloride may be in the form of a salt containing sodium chloride, for example, bamboo salt, bamboo salt, sea salt, refined salt, preparation salt, or pharmacopeia salt.

The said oral composition can contain the well-known additive component which can be used for an oral composition in the range which does not impair the effect of this invention in addition to said each component.

The additive component may be, for example, an abrasive, a binder, a thickener, a surfactant, a sweetener, a preservative, a fragrance, a pharmaceutical ingredient, a colorant, a brightener, a pH adjuster, a solvent, or an excipient, and may be appropriately selected in consideration of the formulation. Although the specific example of an additive component is shown below, the component which the said oral composition can contain is not limited to these.

The abrasive is, for example, silica-based abrasives such as silicic anhydride, crystalline silica, amorphous silica, silica gel, aluminosilicate, zeolite, calcium hydrogen phosphate anhydrate, calcium hydrogen phosphate dihydrate, calcium pyrophosphate, calcium carbonate, aluminum hydroxide , alumina, magnesium carbonate, trimagnesium phosphate, zirconium silicate, tricalcium phosphate, hydroxyapatite, quaternary calcium phosphate, and synthetic resin-based abrasives.

The binder is, for example, pullulan, gelatin, methylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, sodium carboxymethylcellulose, carrageenan, sodium alginate, xanthan gum, sodium polyacrylate, gum arabic, guar gum, locust bean It may be an organic binder such as rubber, polyvinyl alcohol, polyvinylpyrrolidone, and carboxyvinyl polymer, or an inorganic binder such as viscous silica.

The said binder can be used individually by 1 type or in combination of 2 or more type. When using the said binder, the content is 0.01 to 15 weight% with respect to oral cavity composition whole quantity normally, Preferably it is 0.01 to 13 weight%.

The content of the organic binder is 0.01 to 5% by weight, preferably 0.01 to 3% by weight, based on the total amount of the oral composition, the content of the inorganic binder may be 0.1 to 10% by weight based on the total amount of the oral composition.

The viscous agent (wetting agent) may be, for example, sorbitol, propylene glycol, butylene glycol, glycerin, or polyethylene glycol. A viscous agent can be used individually by 1 type or in combination of 2 or more type. When using a viscous agent, the content can be adjusted in the range which does not impair the effect of this invention.

The surfactant may be, for example, an anionic surfactant, a nonionic surfactant, or an amphoteric surfactant.

The anionic surfactant may be, for example, an N-acylamino acid salt, an α-olefinsulfonic acid salt, an N-acylsulfonate salt, an alkyl sulfate salt, or a sulfate salt of a glycerin fatty acid ester. Among these, from the viewpoint of versatility, N-acylamino acid salts, α-olefinsulfonates, and alkyl sulfates are preferable, and from the viewpoint of foamability and hard water resistance, sodium lauroyl sarcosine, the alkyl chain having 10 carbon atoms as the carbon chain length The sodium α-olefinsulfonate of thru|or 16 and sodium lauryl sulfate are more preferable. It is preferable that they are alkali metal salts, such as sodium and potassium, and, as for a salt, it is more preferable that it is a sodium salt.

The nonionic surfactant is, for example, polyoxyethylene alkyl ether, polyoxyethylene-polyoxypropylene block copolymer, polyoxyethylene hydrogenated castor oil, polyoxyethylene fatty acid glycerin ester, sucrose fatty acid ester, fatty acid alkylolamide, glycerin fatty acid It may be an ester or a polyglycerol fatty acid ester. Among these, polyoxyethylene alkyl ether, polyoxyethylene hydrogenated castor oil, fatty acid alkylolamide, and sorbitan fatty acid ester can be used from the viewpoint of versatility.

The polyoxyethylene alkyl ether may have a carbon chain length of an alkyl chain of 14 to 18 carbon atoms. The polyoxyethylene alkyl ether may have an average number of added moles of ethylene oxide from 2 to 30. Polyoxyethylene hydrogenated castor oil may have an average number of added moles of ethylene oxide (average added EO) of 5 to 100. In the fatty acid alkylolamide, the carbon chain length of the alkyl chain may be 12 to 14 carbon atoms. The sorbitan fatty acid ester may have 12 to 18 carbon atoms in the fatty acid. Polyoxyethylene sorbitan fatty acid ester may have 16 to 18 carbon atoms in the fatty acid. In addition, the polyoxyethylene sorbitan fatty acid ester may have an average added mole number of ethylene oxide of 10 to 40.

The amphoteric surfactant may be, for example, a betaine-type amphoteric surfactant, an amino acid-type amphoteric surfactant, or an amine oxide, and a betaine-type amphoteric surfactant is preferred.

The betaine-type amphoteric surfactant may be, for example, an alkylbetaine-type amphoteric surfactant, a fatty acid amide propylbetaine-type amphoteric surfactant, or an alkylimidazolinium betaine-type amphoteric surfactant. Specifically, for example, alkyldimethylaminoacetic acid betaine such as lauryldimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, coconut oil fatty acid amide propyldimethylaminoacetic acid betaine It may be phosphorus, a palm oil fatty acid amide propyl betaine.

The said surfactant can be used individually by 1 type or in combination of 2 or more type. When using the surfactant, its content is, with respect to the total amount of the oral composition, usually 0 to 10% by weight, may be 0.01 to 5% by weight.

The sweetening agent may be, for example, sodium saccharin, stevioside, neohesperidin dihydrochalcone, glycyrrhizin, perillartine, p-methoxycinnamic aldehyde, thaumatin, palatinose, maltitol, xylitol, or arabitol.

The said sweetener can be used individually by 1 type or in combination of 2 or more type. When a sweetener is used, its content may be varied within a range that does not impair the effects of the present invention.

The preservative may be, for example, sodium benzoate, methylparaben, ethylparaben, paraoxybenzoic acid ester such as butylparaben, ethylenediaminetetraacetate, sorbic acid, or sorbate. A preservative can be used individually by 1 type or in combination of 2 or more type.

In the case of using the preservative, its content may be varied within a range that does not impair the effects of the present invention.

The fragrance may be, for example, a natural fragrance, a synthetic fragrance (single fragrance, etc.), a combination fragrance (oil-fat fragrance (oily fragrance), powder fragrance, etc.). A fragrance|flavor can be used individually by 1 type or in combination of 2 or more type.

The natural fragrance is, for example, mastic oil, parsley oil, anise oil, eucalyptus oil, winter green oil, cassia oil, menthol oil, spearmint oil, peppermint oil, lemon oil, coriander oil, orange oil, mandarin oil, lime oil, lavender oil, Laurel oil, chamomile oil, cardamom oil, caraway oil, bay oil, lemongrass oil, pine needle oil, neroli oil, rose oil, jasmine oil, iris concrete, peppermint absort, roseabsolut, orange flower, citrus Milk, mixed fruit oil, strawberry oil, cinnamon oil, clove oil, grape oil, clove oil, thyme oil, sage oil, peppermint oil, rosemary oil, marjoram oil, oliganum oil, grapefruit oil, sweet milk, palm oil, mango It may be a high pressure solution, orange flower absort, red pepper extract, ginger oleoresin, pepper oleoresin, and capsicum oleoresin.

The individual perfume is, for example, carbon, anetol, methyl salicylate, cinnamaldehyde, linalool, linalyl acetate, limonene, menthone, menthyl acetate, pinene, octylaldehyde, citral, fulegon, carbyl acetate, anisaldehyde. , ethyl acetate, ethyl butyrate, allylcyclohexanepropionate, methylanthranilate, ethylmethylanthranilate, vanillin, undecalactone (γ undecalactone, δ undecalactone, etc.), hexanal (trans-2) -Hexenal), ethynone alcohol, propyl alcohol, butanol, isoamyl alcohol, hexenol (cis-3-hexenol, etc.), dimethyl sulfate, cyclotene, furfural, trimethylpyrazine, ethyl lactate, ethylio Acetate, cineol (1,8-cineol, etc.), mentorfuran, linalool oxide, vanillyl butyl ether, isofulegol, praneool, ethylcyclopentenolone, 2-methylbutylic acid, propionic acid, Decalactone (γ decalactone, δ decalactone, etc.), nonalactone (γ nonalactone, δ nonalactone, etc.), hexalactone (γ hexalactone, δ hexalactone, etc.), isoamyl acetate, benzaldehyde, hexyl acetate , Ethyl-2-methylbutyrate, benzyl alcohol, α-terpineol, phenylethyl glycidate, phenylethyl alcohol, allylhexanoate, methylcinnamate, ethylβ-methylthiopropionate, cis-6- It may be nonenol, charon, or methyljasmonate.

The single item may be a cooling agent (冷感劑). The cooling agent is menthol, N-ethyl-p-mentane-3-carboxamide, N-(ethoxycarbonylmethyl)-3-p-mentanecarboxamide, N,2,3-trimethyl-2-isopropylbutane Amide, 3-(L-methoxy)propane-1,2-diol, menthyl lactate (menthyl lactate), monomenthyl succinate, menthone glycerin acetal, 3-l-menthoxypropane-1,2-diol, menthyl glycerin Ethers, spilanthol, and monomentylsuccinate are exemplified.

The combination flavor may be made by combining a single flavor and a natural flavor. For example, the combination flavor is menthol micron, strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, tropical fruit flavor, butter flavor, milk flavor, yogurt flavor, fruit mix flavor, herb mint flavor can be

The form of the perfume is not limited, and any of essential oils, extracts, solids, and powders obtained by spray-drying any one of them may be used. The oral composition usually contains a fragrance material, and the content may be 0.000001 to 1% by weight, respectively, based on the total amount of the oral composition.

The medicinal ingredient is, for example, a caries preventive agent such as fluoride (eg, sodium fluoride, sodium monofluorophosphate, stannous fluoride), chlorhexidine, cetylpyridinium chloride, benzethonium chloride, benzalkonium chloride, zinc gluconate, sterilizing or antibacterial agents such as zinc citrate; calculus preventive agents such as condensed phosphate and ethanehydroxydiphosphonate; Anti-inflammatory agents, such as tranexamic acid, glycyrrhizic acid dipotassium salt, (epsilon) aminocaproic acid, and yellow white extract; Coating agents, such as hydroxyethyl cellulose dimethyl diallyl ammonium chloride; astringents such as allantoin, allantoin chlorhydroxyaluminum, vitamin C, lysozyme chloride, glycyrrhetic acid and its salts, and sodium chloride; It may be a hypersensitivity inhibitor such as strontium chloride, potassium nitrate, or aluminum lactate.

The colorant includes, for example, natural pigments such as safflower red pigment, gardenia yellow pigment, gardenia blue pigment, perilla pigment, red yeast pigment, red cabbage pigment, carrot pigment, hibiscus pigment, cacao pigment, spilulina blue pigment, tamarind pigment, Legal pigments such as Red No. 3, Red No. 104, Red No. 105, Red No. 106, Yellow No. 4, Yellow No. 5, Green No. 3, and Blue No. 1, riboflavin, sodium copper chlorofin, and titanium dioxide may be used.

The brightening agent may be, for example, waxes such as shellac, carnauba wax, and candelilla wax, or calcium stearate.

The pH (20° C.) of the oral composition may be 5 to 10.

The pH adjusting agent is, for example, acetic acid, hydrochloric acid, sulfuric acid, nitric acid, citric acid, phosphoric acid, malic acid, gluconic acid, maleic acid, succinic acid, glutamic acid, sodium hydroxide, potassium hydroxide, sodium acetate, sodium carbonate, sodium citrate, sodium hydrogen citrate, sodium phosphate, acid, alkali or buffer, such as sodium dihydrogen phosphate. When using the said pH adjuster, the content can be suitably adjusted in the range which does not impair the effect of this invention.

The solvent may be, for example, water and a lower alcohol having 3 or less carbon atoms, such as ethanol and propanol.

The excipient may be, for example, starch syrup, glucose, fructose, invert sugar, dextrin, or oligosaccharide. When the said oral composition is a solid food preparation, an excipient can be mix|blended normally. When the excipient is used, its content can be appropriately adjusted within a range that does not impair the effects of the present invention.

Through the following examples, the present invention will be described in more detail, but it is obvious that the present invention is not limited by the following examples.

Preparation Example 2: Preparation of natural extract

After washing each of yellow lily, ginseng, licorice, and green tea leaves, they were dried completely at room temperature, and 100 g of a pulverized product was obtained.

For 100 g of each pulverized product, 20 times the volume of 70% (v/v) ethanol was used as a solvent and immersion extraction was performed at a temperature of 50° C. for 24 hours.

The extract was filtered through a 250 mesh and 3 μm filter, and the remaining raw materials were extracted three times in the same manner, concentrated under reduced pressure at 50° C., and then freeze-dried to obtain a solid.

Preparation Example 1: Preparation of hot water extract

After washing each of yellow lily, ginseng, licorice, and green tea leaves, they were dried completely at room temperature, and 100 g of a pulverized product was obtained.

For 100 g of each pulverized product, 20 times the volume of purified water was used as a solvent and immersion extraction was performed at a temperature of 60° C. for 24 hours.

The extract was filtered through a 250 mesh and 3 μm filter, and the remaining raw materials were extracted three times in the same manner, concentrated under reduced pressure at 50° C., and then freeze-dried to obtain a solid.

Preparation Example 2: Preparation of ethanol extract

An extract was prepared in the same manner as in Preparation Example 1, except that 70% (v/v) ethanol was used as the extraction solvent.

Examples and Comparative Examples

An oral composition was prepared by changing the components as shown in Table 1 below. As other additives, small amounts of alatonin, glycerin, sodium citrate, maltitol, xylitol, sodium benzoate and the like were added.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 Yellow lily extract (Preparation Example 1) 150 100 100 75 90 - - - Ginseng extract (Preparation Example 1) 150 100 100 75 90 - - - Licorice extract (Preparation Example 1) - 100 - 75 60 - - - Green tea extract (Preparation Example 1) - - 100 75 60 - - - Yellow lily extract (Preparation Example 2) - - - - - 150 75 90 Ginseng extract (Preparation Example 2) - - - - - 150 75 90 Licorice extract (Preparation Example 2) - - - - - - 75 60 Green tea extract (Preparation Example 2) - - - - - - 75 60 Purified water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount other additives

[Content (parts by weight)] division comparative example One 2 3 4 5 6 7 8 Yellow lily extract (Preparation Example 1) 300 - - 150 150 100 - Ginseng extract (Preparation Example 1) - 300 - - - - - 100 Licorice extract (Preparation Example 1) - - 300 - 150 - 100 100 Green tea extract (Preparation Example 1) - - - 300 - 150 100 100 Purified water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount other additives

Experimental Example 1: Evaluation of dental plaque index ( In-vivo )

The dental plaque index for the mouthwashes of Examples and Comparative Examples was evaluated.

After confirming the initial oral condition of 80 subjects, the teeth of the subjects were cleaned using an ultrasonic tartar remover.

After 2 weeks, the mouthwashes of Examples and Comparative Examples were divided into 16 groups of 5 persons each, and then used before bedtime, and the plaque index was examined 4 weeks later and 12 weeks later.

As for the dental biofilm index test method, the following scores were given according to the distribution of plaque after staining with a coloring solution for the test.

0 points: When the dental plaque is not attached at all

1 point: If there is a dentate plaque on the gingival margin

2 points: When there is an annular, 1 mm or less dental plaque in the cervical region

3 points: When there is an annular dental plaque of 1 mm or more on the tooth surface less than 1/3 of the cervical side

4 points: If there is a dental plaque on the tooth surface less than 2/3 of the cervical side

5 points: When there is a dental plaque on the tooth surface of more than 2/3 of the cervical side

Bacterial film index on teeth = total score / number of graded teeth

According to the evaluation method, the average value of the change in the biofilm index after 4 weeks and 12 weeks was calculated (Table 3).

division 4 weeks 12 weeks Example 1 -0.41 -0.81 Example 2 -0.44 -0.84 Example 3 -0.43 -0.85 Example 4 -0.53 -0.96 Example 5 -0.56 -0.98 Example 6 -0.45 -0.83 Example 7 -0.58 -0.95 Example 8 -0.59 -1.01 Comparative Example 1 -0.24 -0.40 Comparative Example 2 -0.22 -0.36 Comparative Example 3 -0.25 -0.34 Comparative Example 4 -0.16 -0.26 Comparative Example 5 -0.23 -0.27 Comparative Example 6 -0.31 -0.40 Comparative Example 7 -0.32 -0.41 Comparative Example 8 -0.29 -0.39

Referring to Table 3, the oral composition (mouthwash) of the Example containing both the yellow lily extract and the ginseng extract at the same time was excellent in inhibiting the formation of dental plaque compared to the comparative example, and the activity was further improved by the addition of licorice extract and green tea extract ( Examples 2 to 5).

In particular, Example 5, in which the mixing ratio was changed to 3:3:2:2, was more active than Example 4 in which the mixing ratio was 1:1:1:1, and the activity of Examples 6 to 8 with the addition of the ethanol extract The sample was more excellent in inhibiting the formation of dental plaque compared to the hot water extract (Examples 1, 4, 5).

On the other hand, the oral composition of the comparative example was evaluated at a lower level compared to the example, although some of the dental plaque inhibitory activity was confirmed.

The above results suggest that synergistic activity can be realized by the simultaneous action of the Hwangryeon extract and the ginseng extract, and the activity can be further improved when the extract is prepared through ethanol.

Experimental Example 2: Evaluation of anti-inflammatory activity

The anti-inflammatory activity of Examples and Comparative Examples was evaluated by analyzing the NO production inhibitory ability of EAME in RAW 264.7 cell, a mouse macrophage cell line induced by lipopolysaccharide (LPS) stimulation.

For the experiment, Raw 264.7 cells, a murine macrophage cell line, were distributed from the Korean cell line bank and cultured in a _{5% CO 2} incubator at 37°C using DMEM medium containing 100 units/mL penicillin-streptomycin and 10% fetal bovine serum (FBS). and subculture was performed once every 2-3 days.

Raw 264.7 cells (3 X 10 ⁵ cells/mL) were cultured 18 hours before, and the samples of Examples and Comparative Examples (concentration 10%) and LPS (1 μg/mL) were simultaneously treated and cultured for 24 hours.

The generated NO was measured in the form of _{NO 2} ⁻ present in the cell culture medium using Griess reagent.

The negative control group was not treated with LPS, and the positive control group was treated with only LPS to measure the amount of NO generated.

100 μL of cell culture supernatant and Griess reagent (1% (w/v) sulfanilamide, 0.1% (w/v) naphtylehtylenediamine in 2.5% (v/v) phosphoric acid) were mixed in equal amounts and reacted in the dark at room temperature for 10 minutes, followed by ELISA Absorbance (540 nm) was measured using a reader.

The standard concentration curve was _{obtained by serial dilution of sodium nitrite (NaNO 2} ) (10-100 μM).

division NO production (μM) Example 1 14.89 Example 2 13.05 Example 3 12.75 Example 4 12.05 Example 5 11.53 Example 6 14.29 Example 7 11.15 Example 8 10.24 Comparative Example 1 21.77 Comparative Example 2 24.18 Comparative Example 3 22.73 Comparative Example 4 21.77 Comparative Example 5 24.08 Comparative Example 6 19.38 Comparative Example 7 19.19 Comparative Example 8 18.95 LPS(-) 6.53 LPS(+) 37.24

Referring to Table 4, the oral composition (mouthwash) of the Example containing both the yellow lily extract and the ginseng extract simultaneously exhibited a higher NO (nitric oxide) production inhibitory activity than the comparative example, and was active by the addition of licorice extract and green tea extract. This was further improved (Examples 2 to 5).

In particular, Example 5, in which the mixing ratio was changed to 3:3:2:2, further increased anti-inflammatory activity than Example 4 in which the mixing ratio was 1:1:1:1, and Examples 6 to 6 to which the ethanol extract was added. The sample of No. 8 was more excellent in inhibiting NO (nitric oxide) production compared to the hot water extract (Examples 1, 4, 5).

In the case of the oral composition of Comparative Example, some anti-inflammatory activity was confirmed, but it was evaluated at a lower level than in Examples.

The above results suggest that the anti-inflammatory activity can be further improved by the simultaneous action of the H. rhododendron extract and the ginseng extract, and the anti-inflammatory activity can be further enhanced when the extract is prepared through ethanol.

Experimental Example 3: Evaluation of antibacterial activity

The antibacterial activity of the samples of Examples and Comparative Examples was evaluated.

S. Mutans and P. Gingivitis were struck in a medium optimized for growth, respectively, and cultured for 2 to 3 days, prepared in advance by McFaland nephleometer standards 0.5 (1% Barum chloride (0.05 ml) + It was the same as the turbidity of 1% Sulfuric acid (9.95 ml)).

After diluting each sample to the same concentration in 2ml of liquid BHI medium, ^{20μL of 1x10 8} cells/mL of the bacterial solution was inoculated and incubated for 48 hours.

Based on the turbidity, the minimum concentration inhibiting growth was defined as the minimum growth inhibitory concentration (MIC), and the results are shown in Table 5 below.

division Minimum growth inhibitory concentration MIC (PPM) mutans bacteria ( S. Mutans ) Gingivitis ( P. Gingivitis ) Example 1 610 550 Example 2 550 490 Example 3 540 480 Example 4 520 460 Example 5 500 440 Example 6 570 510 Example 7 490 430 Example 8 470 410 Comparative Example 1 1030 970 Comparative Example 2 1180 1120 Comparative Example 3 1050 990 Comparative Example 4 1030 970 Comparative Example 5 1180 1120 Comparative Example 6 1040 980 Comparative Example 7 970 910 Comparative Example 8 940 880 Comparative Example 9 1010 950 Comparative Example 10 880 820 Comparative Example 11 920 860 Comparative Example 12 900 840

Referring to Table 5, the oral composition of the Example containing both the Hwangryeon extract and the ginseng extract simultaneously had excellent antibacterial activity compared to the comparative example, and the antibacterial activity was further enhanced by the addition of the licorice extract and green tea extract (Examples 2 to 5) .

In particular, Example 5, in which the mixing ratio was changed to 3:3:2:2, further increased antibacterial activity than Example 4 in which the mixing ratio was 1:1:1:1, and Examples 6 to 8 in which the ethanol extract was added. of the sample had better antibacterial activity compared to the hot water extract (Examples 1, 4, 5).

The above results suggest that synergistic activity can be realized by the simultaneous action of the Hwangryeon extract and the ginseng extract, and the antibacterial activity can be further improved when the extract is prepared through ethanol.

Experimental Example 4: Evaluation of halitosis inhibitory activity

Halitosis inhibitory activity was measured in 80 healthy subjects who had no specific medication history within the last 6 months.

A 3 mM cysteine solution, Examples and Comparative Examples, and Gargreen mint solution were used as a positive control, and Oral Chroma (CHM-1, ABILITO, Japan) was used for bad breath measurement.

In order to measure the basic halitosis level twice at 5-minute intervals prior to each experiment, a baseline was established.

The control group gargled with 10 ml of 3 mM cysteine solution for 30 seconds to induce bad breath, and then collected oral gas with a syringe.

After inserting a syringe that collected intraoral gas into the inlet of Oral Chroma, and injecting 0.5cc of the collected gas, the concentration of volatile sulfur compound (VSC) was recorded.

In addition, the experimental group performed the same procedure as the control group with a 3 mM cysteine solution, and after 4 minutes, the samples of Examples and Comparative Examples were gargled for 60 seconds, bad breath was collected, and the concentrations of hydrogen sulfide, methyl mercaptan, and dimethyl sulfide were measured.

All individual experiments were repeated three times, and the results were expressed as VSC relative ratios by dividing the concentration of VSC by the reference value measured before each experiment. The results are shown in Table 6 below.

division hydrogen sulfide (H ₂ S) Methyl mercaptan (CH ₃ SH) dimethyl sulfide ((CH ₃ ) ₂ S) Example 1 0.72 0.18 0.48 Example 2 0.63 0.16 0.42 Example 3 0.61 0.15 0.40 Example 4 0.55 0.14 0.36 Example 5 0.51 0.13 0.34 Example 6 0.61 0.15 0.40 Example 7 0.53 0.13 0.35 Example 8 0.49 0.12 0.33 Comparative Example 1 2.13 0.52 0.94 Comparative Example 2 1.96 0.65 0.91 Comparative Example 3 1.75 0.65 0.86 Comparative Example 4 2.36 0.54 0.97 Comparative Example 5 2.53 0.46 0.75 Comparative Example 6 1.25 0.53 0.82 Comparative Example 7 1.45 0.42 0.86 Comparative Example 8 1.53 0.48 0.79 positive control 2.02 0.45 0.75 control 3.73 0.73 1.12

Referring to Table 6, the oral composition of the Example containing both the yellow lily extract and the ginseng extract at the same time had excellent halitosis inhibitory activity compared to the comparative example, and the halitosis inhibitory activity was further enhanced by the addition of licorice extract and green tea extract (Examples 2 to 5).

The oral composition of Examples containing both the yellow lily extract and the ginseng extract simultaneously had excellent halitosis inhibitory activity compared to the comparative example, and the halitosis inhibitory activity was further enhanced by the addition of the licorice extract and green tea extract (Examples 2 to 5).

In particular, Example 5, in which the mixing ratio was changed to 3:3:2:2, had better halitosis inhibitory activity than Example 4 in which the mixing ratio was 1:1:1:1. This was further enhanced (Examples 6 to 8).

Experimental Example 5: Evaluation of periodontal disease inhibitory activity ( In-vivo )

For patients with gingivitis with even dentition and no missing teeth, precise oral examinations were performed according to gender at 10-year intervals from 30 to 50 years of age by age, and 80 subjects were selected.

The mouthwashes of Examples and Comparative Examples were divided into 16 groups of 5 persons each and provided, and then used before bedtime, and the gingivitis index was examined 1 month and 3 months later.

The gingivitis index is measured by inserting a periodontal probe into the gingival sulcus, burning the probe around each tooth without applying any force, and measuring the bleeding state after 30 seconds have elapsed. The results were calculated by recording.

0 points: No bleeding

1 point: Normal bleeding

2 points: glandular bleeding

3 points: Triangular bleeding in the interdental region

4 points: Total gingival bleeding

According to the evaluation method, the average value of the gingivitis index change after 1 month and 3 months was calculated (Table 7).

division 1 month passed 3 months passed Example 1 -0.41 -0.88 Example 2 -0.47 -0.93 Example 3 -0.49 -0.95 Example 4 -0.55 -1.02 Example 5 -0.59 -1.12 Example 6 -0.45 -0.93 Example 7 -0.60 -1.07 Example 8 -0.67 -1.17 Comparative Example 1 -0.21 -0.38 Comparative Example 2 -0.19 -0.35 Comparative Example 3 -0.23 -0.34 Comparative Example 4 -0.11 -0.28 Comparative Example 5 -0.12 -0.30 Comparative Example 6 -0.29 -0.39 Comparative Example 7 -0.33 -0.43 Comparative Example 8 -0.31 -0.45

Referring to Table 7, the oral composition of the Example containing both the yellow lily extract and the ginseng extract simultaneously had excellent gingivitis inhibitory activity compared to the comparative example, and the gingivitis inhibitory activity was further enhanced by the addition of licorice extract and green tea extract (Examples 2 to 5).

In particular, Example 5, in which the mixing ratio was changed to 3:3:2:2, had better gingivitis inhibitory activity than Example 4 in which the mixing ratio was 1:1:1:1, and Examples 6 to 6 to which the ethanol extract was added. The sample of No. 8 had better gingivitis inhibitory activity than the hot water extract (Examples 1, 4, 5).

The above results suggest that synergistic activity can be realized by the simultaneous action of the extract of H.

The description of the present invention described above is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (8)

Hwangryeon extract, licorice extract, green tea extract and ginseng extract in a weight ratio of 3:3:2:2,
Including antibacterial use against P. Gingivitis,
anti-inflammatory, anti-tartar and anti-halitosis use;
The extract is an ethanol extract at a concentration of 60 to 80% (v/v), an oral composition for improving periodontal disease. delete delete delete According to claim 1,
An oral composition further comprising an antibacterial use against S. Mutans. delete delete According to claim 1,
An oral composition formulated with one or more selected from the group consisting of mouthwash, toothpaste, oral spray, oral ointment, oral patch, and gum.