JP5892716B2 - Oral composition - Google Patents

Description

  The present invention relates to an oral composition that has an excellent GTase inhibitory action and can effectively prevent dental caries by suppressing the formation of dental plaque.

  As one of the diseases in the oral cavity, caries (tooth decay) is known. The occurrence mechanism of this caries is considered as follows. On the tooth surface, a pellicle layer is formed by glycoprotein which is a component in saliva, and bacteria such as mutans bacteria adhere to the pellicle layer. This bacterium is modified by the action of glucosyltransferase (hereinafter referred to as “GTase”), an enzyme that is secreted outside the cell body, to denature food-derived sucrose into an adhesive polysaccharide, and further grows firmly in the teeth. Adhere to the surface. This is generally called plaque, but the attached bacteria metabolize saccharides in plaque and produce acid as a metabolite. This acid then decalcifies the tooth enamel surface, causing caries. In addition to dental caries, plaque is said to cause halitosis, periodontal disease, gingivitis, and alveolar pus leakage.

Today, it is considered effective to inhibit the formation of dental plaque by inhibiting the action of GTase as one measure for preventing dental caries. From such a viewpoint, in recent years, a substance having a GTase inhibitory action has been vigorously developed, and various plant extracts having a GTase inhibitory action have been reported (for example, see Patent Document 1). However, many of the plant extracts reported so far cannot be put to practical use for reasons such as insufficient GTase inhibitory activity, and development and provision of further GTase inhibitory components and oral compositions using the same. Is desired.
Japanese Patent Laid-Open No. 4-95020

  Therefore, an object of the present invention is to provide an oral composition that has an excellent GTase inhibitory action and can effectively prevent dental caries by suppressing the formation of plaque.

  The present inventor has intensively studied to solve the above-mentioned problems. As a result, the polar solvent extract of the plant of the genus Ranaaceae has an excellent GTase inhibitory action. It was found that it is useful for prevention of pitting. The present invention has been completed by making further improvements based on such findings.

That is, this invention includes the invention of the aspect hung up below:
Item 1. A composition for oral cavity for anti-caries , comprising a polar solvent extract of a plant of the genus Rubiaceae.
Item 2. Item 2. The oral cavity composition for caries according to Item 1, which contains 0.00001 to 20% by weight of a polar solvent extract of the plant of the genus Rubiaceae.
Item 3. Item 3. The oral cavity composition for caries according to Item 1 or 2 , wherein the extract is an extract of a seed of the genus Oleaceae.
Item 4. Item 4. The oral cavity composition for anti-carious disease according to any one of Items 1 to 3 , wherein the plant of the genus Rabbitaceae is an evening primrose.

The composition for oral cavity of the present invention is characterized by containing a polar solvent extract of a plant of the genus Oenothera .

The onagraceae evening primrose genus plants used in the present invention is not particularly limited, for example, evening primrose (Oenothera biennis), evening primrose (O enothera striata), evening primrose (Oenothera erythrosepala), Oenothera laciniata (Oenotheralaciniata) and the like . Of these, the evening primrose is preferable.

  The extract of the genus Oenothera spp. Is not limited as long as it is extracted from the whole plant of the plant or a part of the plant such as seeds, roots, stems, leaves, and florets. Extracted from plant seeds.

  Moreover, as a solvent used for the extraction of the extract of the genus Ranunculaceae, there is no particular limitation as long as it is a polar solvent. Examples of such polar solvents include water; lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propanol, isopropyl alcohol, and butanol; polyhydric alcohols such as 1,3-butylene glycol, glycerin, and polyethylene glycol. Acetone; ethyl ether; ethyl acetate; methyl acetate and the like. These polar solvents may be used alone or in combination of two or more. The extraction solvent is preferably water, a lower alcohol having 1 to 5 carbon atoms, and a mixed liquid of water having a lower alcohol having 1 to 5 carbon atoms and water; more preferably water, ethanol, and a mixed liquid of ethanol and water; Is a mixture of ethanol and water. When a mixed solution of lower alcohol having 1 to 5 carbon atoms and water is used as the extraction solvent, the content ratio of the lower alcohol to the total weight of the mixed solution is, for example, 0.0001 to 99.9% by weight, preferably Is a ratio of 40 to 99.9% by weight, more preferably 50 to 90% by weight.

  The extract of the genus Rhizophoraceae is obtained by extracting the plant part to be extracted as it is or after drying, shredded, crushed, pressed, boiled or fermented with the above extraction solvent. It is done. As the extraction method, a commonly used extraction method for plant extracts can be employed, and specifically, immersion methods such as cold immersion and digestion; a method of stirring under heating; or a percolation method, etc. Is exemplified.

  In addition, prior to the extraction of the genus Avenidae with a polar solvent, as a pretreatment, a degreasing treatment is performed in advance by using a non-polar organic solvent such as a pressing method or hexane, and an extra portion is removed from the plant during the extraction treatment. It is desirable to prevent the extraction of fresh lipids.

  The extract of the plant of the genus Rubiaceae obtained by the above method is in a liquid state, and in the present invention, the extract may be used in a liquid state, and it is concentrated by subjecting it to a treatment such as concentration and drying as necessary. You may use as a thing or a dry thing. Further, after concentration or drying, the concentrated or dried product may be used after being purified by washing with an insoluble solvent, or may be used by further dissolving or suspending it in an appropriate solvent. It is also possible to obtain and purify a fraction having GTase inhibitory activity using the obtained extract using a conventional purification method such as a countercurrent distribution method or liquid chromatography. .

  In addition, for convenience, a commercially available extract can be used as the extract of the plant of the genus Ranunculaceae.

  In the composition for oral cavity of the present invention, the blending ratio of the extract of the genus Acanthaceae, which depends on the form of the composition for oral cavity, the concentration rate of the extract, etc., cannot be defined uniformly. As an example, the extract (in terms of dry weight) is usually 0.00001 to 20% by weight, preferably 0.0001 to 10% by weight, more preferably 0.001 to 5%, based on the total amount of the composition for oral cavity. The ratio which becomes weight% is illustrated.

The composition for oral cavity of the present invention has an anti-cariogenic effect by combining it with an antibacterial agent against carious bacteria and other GTase inhibitors in addition to the above-mentioned extract of the genus Rhizopus. It can also be strengthened synergistically or synergistically. Examples of such antibacterial agents include chlorhexidine hydrochloride, cetylpyridinium chloride, sorbic acid, hinokitiol, isopropylmethylphenol, decalinium chloride, triclosan, chlorhexidine hydrochloride, potassium iodide and the like. Other GTase inhibitors include, for example, Vitis plant extracts, dextranase, mutanase, sodium monofluorophosphate, sodium fluoride, stannous fluoride, tastein, tannins , Ellagic acid, polyphenol, oolong tea extract, green tea extract, assembly, Taiso, fennel, glaze, gentian, senso, rye gall, yellow ream and the like.

  As a preferred embodiment of the composition for oral cavity of the present invention, in addition to the polar solvent extract of the above-mentioned Aceraceae Oenothera genus plant, a composition further containing an extract of a Grapeaceae genus plant as a GTase inhibitor is exemplified. The

The grapevine genus plant is not particularly limited, and European grape ( Vitis vinifera L.), American grape ( Vitis labrusca L.) and the like can be used. Preferred is European grape.

  Although the extract of the grape genus Grapes plant should just be extracted from the leaf part, stem part, root part, or these mixture of this plant body, Preferably it is extracted from the leaf part.

  In addition, the solvent used for the extraction of the extract of the genus Grapeaceae is not particularly limited. For example, water; lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propanol, isopropyl alcohol, butanol; Examples include polyhydric alcohols such as 1,3-butylene glycol, glycerin and polyethylene glycol; polar solvents such as acetone; ethyl ether; ethyl acetate; These solvent may be used individually by 1 type, and may be used in combination of 2 or more type. The extraction solvent is preferably water, a lower alcohol having 1 to 5 carbon atoms, and a mixed liquid of water having a lower alcohol having 1 to 5 carbon atoms and water; more preferably water, ethanol, and a mixed liquid of ethanol and water; Is water. When a mixed liquid of a lower alcohol having 1 to 5 carbon atoms and water is used as the extraction solvent, the content ratio of the lower alcohol to the total weight of the mixed liquid is, for example, 0.1 to 99.9% by weight, preferably Is 0.1 to 70% by weight, more preferably 0.1 to 50% by weight.

  Extraction of an extract of a vine belonging to the genus Grapeaceae can be performed by a method similar to the case of the extract of the genus Acanthaceae. Prior to solvent extraction of Grapeaceae plants, as a pretreatment, the plant may be preliminarily degreased by using a pressing method or a nonpolar organic solvent such as hexane.

  As in the case of the above-mentioned extract of the genus Aceraceae, the extract of the grape genus grape genus plant may be liquid, or may be in the form of a concentrate or a dried product.

  When blending the above-mentioned grapevine plant extract in the oral composition of the present invention, the blending ratio of the extract is not particularly limited, but is, for example, 0.00001 to 20% by weight, preferably 0.8. A ratio of 0001 to 10% by weight, more preferably 0.001 to 5% by weight, is mentioned.

  The composition for oral cavity of the present invention may contain components usually used in the composition for oral cavity depending on its form. Examples of such components include, but are not limited to, abrasives, binders, thickeners, foaming agents, fragrances, flavoring agents, sweeteners, preservatives, anti-inflammatory agents, disinfectants, antibacterial agents, dyes, Examples thereof include a viscosity agent, a surfactant, a pH adjuster, and an antioxidant.

In the present invention, the oral composition, is intended to include both compositions that are used in the oral cavity as through the mouth to ingested composition, and toothpaste and mouthwash, for example.

Specific examples of the oral composition of the present invention, edible films, lozenges, the tablet, granule, capsule Le; drinks; dentifrices (kneaded-like, liquid, powder solid), breath fresheners (mouth spray Etc.), chewing agents, lozenges, oral pastes, gargles, syrups, gingival massage creams and other drugs or quasi-drugs; oral cosmetics such as dentifrices, mouthwashes, mouth rinses, etc. . Preferably Among these may be mentioned edible films, dental Migakuzai, breath fresheners, mouthwash (mouth wash), a mouthrinse or the like. These forms and dosage forms are not particularly limited, and can be arbitrarily determined according to the type.

Moreover, the composition for oral cavity of this invention can show effects, such as a GTase inhibitory effect, a plaque formation inhibitory effect, an anti-caries effect, by the effect | action of the polar-solvent extract of the Acrobaceae evening primrose plant to contain. That is, the oral composition of the present invention is useful as a GTase inhibitor, a plaque formation inhibitor, or an anti-cariogenic agent. Furthermore, the present invention provides the following aspects of the invention from another viewpoint:
(1) A GTase inhibitor, a plaque formation inhibitor or an anti-cariogenic agent, characterized by containing a polar solvent extract of a plant belonging to the genus Rubiaceae.
(2) The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to (1), wherein the extract is an extract of the seeds of the genus Oenothera spp.
(3) The GTase inhibitor, plaque formation inhibitor, or anti-cariogenic agent according to (1) or (2), wherein the plant of the genus Ranunculaceae is the evening primrose.
(4) The above-mentioned extract of the genus Ranaaceae is 0.00001 to 20% by weight, preferably 0.0001 to 10% by weight, more preferably 0.001 to 5% by weight, based on the total amount of the agent. The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to any one of (1) to (3), which is contained in a proportion of
(5) In addition, the GTase inhibitor according to any one of (1) to (4), further comprising an extract of a leaf part, a stem part, a root part, or a mixture thereof, of a Grapeaceae genus plant, plaque formation inhibition Or anti-cariogenic agent.
(6) The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to (5), wherein the extract of the Grapeaceae genus plant is obtained using a polar solvent.
(7) The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to (5) or (6), wherein the Grapeaceae genus plant is European grape.
(8) The extract of the Grapeaceae genus plant is 0.00001 to 20% by weight, preferably 0.0001 to 10% by weight, more preferably 0.001 to 5% by weight, based on the total amount of the agent. The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to any one of (5) to (7), which is contained in a proportion of
(9) The GTase inhibitor, plaque formation inhibitor or anti-cariogenic agent according to any one of (1) to (8), which is an oral composition.
(10) and Drug, a quasi drug or oral cosmetics, GTase inhibitors, plaque formation inhibitor or anti-dental caries agent according to (9).

  In addition, in the above GTase inhibitor, plaque formation inhibitor, and anti-cariogenic agent, the polar solvent extract of the plant of the genus Aceraceae that is used, the blending ratio of the extract, the components that can be blended in the inhibitor, the form of use, etc. About the case of the composition for oral cavity mentioned above can be used.

  According to the oral composition of the present invention, an excellent GTase inhibitory effect can be achieved in the oral cavity based on the action of the polar solvent extract of the genus Oenothera spp., Thereby inhibiting plaque formation. Caries can be effectively prevented.

Moreover, the polar solvent extract of the plant of the genus Rubiaceae that is blended in the composition for oral cavity of the present invention is a naturally derived component and has high safety .

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited to these Examples.

Production Example 1
100 g of pulverized seeds of Oenothera biennis plant were degreased according to a conventional method, and the residue was collected by filtration. After the defatted defatted seed pulverized product was dried, extraction was performed at 45 to 55 ° C. for 2 hours using a water ethanol mixed solution (containing 30 wt% water and 70 wt% ethanol) as an extraction solvent. Next, the residue was removed by filtration to obtain an extract, which was concentrated with an evaporator and spray-dried to obtain 5 g of a plant extract (hereinafter referred to as “Oenothera plant extract”).

Reference production example 1
Extraction processing was performed by a dipping method using a ground product of European grape ( Vitis vinifera L.) as a raw material and purified water as an extraction solvent. After the extraction treatment, the residue was removed by filtration to obtain an extract, which was concentrated and spray-dried with an evaporator to obtain a plant extract (hereinafter referred to as “Grape plant extract”).

Test Example 1 Evaluation of inhibitory effect on GTase produced by Streptococcus sobrinus
In order to evaluate the inhibitory action of Oenothera genus plant extracts on GTase produced by Streptococcus sobrinus 6715 (distributed from Osaka University School of Dentistry), the following test was conducted.
1. Preparation of Streptococcus sobrinus 6715 production GTase
(1) Preparation of medium
Water was added to 15 g of Trypticase, 4 g of Tryptose, 4 g of yeast extract, ₂ g of K ₂ HPO ₄ , 5 g of KH ₂ PO ₄ , ₂ g of Na ₂ CO ₃ , and NaCl 2 g to make a total of 900 ml. Separately, water was added to 10 g of glucose to make a total of 100 ml. After these were heat sterilized separately and before culturing, the medium was prepared by mixing them.
(2) a Streptococcus sobrinus 6715 strain Streptococcussobrinus 6715 strain that had been pre-cultured culture was inoculated into the above medium 1000 ml, 18 hours at 37 ° C., was stationary culture.
(3) Purification of GTase The culture solution obtained in (2) above was centrifuged, and the supernatant was recovered. Ammonium sulfate was added to the obtained supernatant to a final concentration of 50% by weight, and then centrifuged again to collect the precipitate. The collected precipitate was washed with a phosphate buffer (pH 6.5) and then centrifuged again to collect the precipitate. An appropriate amount of phosphate buffer (pH 6.5) was added to the resulting precipitate, and dialysis was performed using the same buffer. After dialysis, a solution containing GTase was collected and used as a GTase solution. The obtained GTase solution was stored at −80 ° C.

2. Evaluation test of inhibitory action against Streptococcus sobrinus 6715 production GTase In each hole of 96-well plate, substrate solution (0.1 M phosphate buffer (pH 6.5), 2 wt% sucrose, 0.1 wt% sodium azide, 40 μM dextran 10 (Molecular weight 10,000) included) 260 μl, GTase solution 20 μl, and Oenothera plant extract solution (adjusted so that the final concentration of Oenothera plant extract in the hole is 0.063-2 mg / ml) are added, 37 Incubated for 18 hours at ° C. Thereafter, the absorbance at 550 nm was measured in order to determine the amount of synthesized glucan. Further, as a control, the same measurement was performed on a hole to which no extract of the genus Oenothera was added. Taking the absorbance at 550 nm of the control as the GTase activity value of 100%, the GTase activity value (%) in the presence of the extract of the genus Oenothera plant at each concentration was calculated. For comparison, a test was performed in the same manner as described above, using a oolong tea extract (trade name “San Oolong” manufactured by Suntory Ltd.) with a known GTase inhibitory activity. For reference, a test was carried out in the same manner as described above using a grape plant extract.

  The obtained results are shown in FIG. From this result, it was confirmed that the Oenothera genus plant extract has a significantly superior GTase inhibitory action compared to the oolong tea extract, and it is clear that the extract can exhibit an effective anti-cariogenic action. became. In addition, it was confirmed that the grape plant extract evaluated for reference has an excellent GTase inhibitory action.

Test Example 2 Streptococcus sobrinus further assessment of inhibitory effect against GTase produced, for Streptococcus sobrinus 6715 strain (dispensing from Osaka Dental) to evaluate the inhibitory effect of evening primrose genus plant extract on GTase produced, in Test Example In the same manner as in Example 1 (however, the final concentration in the well of the extract was 0.5 to 2 mg / ml), the inhibitory effect of the evening primrose plant extract on GTase was evaluated. Here, for comparison, a green tea extract (trade name “Green Tea Dried Extract”, Ask Chemical Co., Ltd.) was used for the same test. For reference, a grape plant extract was similarly tested.

  The obtained results are shown in FIG. From this result, it is clear that the Oenothera plant extract has a significantly superior GTase inhibitory action compared to the green tea extract with known GTase inhibitory activity, It was confirmed that the caries action can be effectively exhibited.

Test Example 3 Evaluation of inhibitory action on GTase produced by Streptococcus mutans
The inhibitory action of evening primrose extract on GTase produced by Streptococcus mutans MT8148 strain (distributed from Osaka University Dental School) was evaluated. Specifically, Streptococcusmutans MT8148 strain-produced GTase was prepared by the method described below, and an evaluation test of the inhibitory effect of evening primrose plant extract on the GTase was performed by the same method as in Test Example 1.
<Preparation of Streptococcus mutans MT8148 strain production GTase>
(1) Preparation of medium
Water was added to 15 g of Trypticase, 4 g of Tryptose, 4 g of yeast extract, ₂ g of K ₂ HPO ₄ , 5 g of KH ₂ PO ₄ , ₂ g of Na ₂ CO ₃ , and NaCl 2 g to make a total of 900 ml. Separately, water was added to 10 g of glucose to make a total of 100 ml. After these were heat sterilized separately and before culturing, the medium was prepared by mixing them.
(2) the Streptococcus mutans MT8148 strain Streptococcusmutans MT8148 strain which had been precultured culture was inoculated into the above medium 1000 ml, 18 hours at 37 ° C., was stationary culture.
(3) Purification of GTase The culture solution obtained in (2) above was centrifuged, and the precipitate was collected. The obtained precipitate was washed with a phosphate buffer (pH 6.5) and then centrifuged again to collect the precipitate. Next, 8M urea was added to the collected precipitate for extraction. The obtained supernatant was dialyzed with a phosphate buffer (pH 6.5) and subjected to ultrafiltration to obtain a GTase solution. The obtained GTase solution was stored at −80 ° C.

  The obtained results are shown in FIG. From this result, as in Test Example 1, it was confirmed that the evening primrose plant extract has an excellent GTase inhibitory action. In addition, it was confirmed that the grape plant extract evaluated for reference also has an excellent GTase inhibitory action.

Test Example 4 Evaluation of Plaque Formation Inhibitory Action In order to evaluate the plaque formation inhibitory action of an extract of the genus Oenothera plant, 12 subjects (25-30 year old men without periodontal disease) The following tests were conducted using the mouthwashes shown (Example 1 and Comparative Example 1).

  First, dental plaque in the oral cavity was completely removed using an electric toothbrush or dental floss. It was confirmed that plaque in the oral cavity was completely removed using a plaque staining solution (trade name “DENT Liquid Plaque Tester”; manufactured by Lion Corporation). The mouthwash was carried out for 2 days using the mouthwash of Example 1. The mouthwash was performed with 10 ml of the mouthwash of Example 1 for 20 seconds before eating, after eating, before going to bed, before snacking, and after snacking. During the period, the subject was prohibited from physical or chemical oral cleaning except for the mouthwash of Example 1. Japanese tea, tea, coffee and wine were also prohibited, but other foods, sweeteners and smoking were not restricted. Two days after the start of use of the mouthwash of Example 1, the state of adhesion of plaque was confirmed. After the test period using the mouthwash of Example 1 was completed, the mouthwash was performed under the same conditions using the mouthwash of Comparative Example 1 with an interval of 5 days, and the adhesion status of plaque was determined. confirmed.

Evaluation of plaque adhesion was performed on the maxillary right first molar, maxillary left central incisor, maxillary left first premolar, mandibular left first premolar, mandibular right central incisor, and mandibular right first premolar. After staining with dental plaque staining solution (trade name “DENT Liquid Plaque Tester”; manufactured by Lion Corporation), both cheek and palate sides are observed using a mirror holder and the following criteria are used: Was scored according to
<Criteria for determining plaque adhesion>
Plaque score 0: No stained area 1: Slightly stained area 2: Area of 1/3 or less of the surface area is stained 3: Area greater than 1/3 of the surface area and 2/3 or less 4: Area larger than 2/3 of the surface area is stained For reference, Fig. 4 shows a representative example of the relationship between plaque score and plaque adhesion (stained area). .

In addition, after the above test was completed, the subjects were evaluated for the feeling of use of the mouth washes of Example 1 and Comparative Example 1 by scoring “actual feeling of plaque formation inhibiting effect” and “taste” according to the following criteria.
<Judgment criteria for feeling of mouthwash>
・ Real feeling of plaque formation inhibitory effect 5: Example 1 feels that plaque formation inhibitory effect is superior to Comparative Example 1. 4: Example 1 has better plaque formation inhibitory effect than Comparative Example 1. 3: I feel that the plaque formation inhibitory effect is the same between Example 1 and Comparative Example 2. 2: The plaque formation inhibitory effect of Example 1 is slightly higher than that of Comparative Example 1. I feel that it is inferior 1: I feel that Example 1 is inferior in plaque formation inhibition effect compared with Comparative Example 1
・ Taste

5: Example 1 feels better in taste than Comparative Example 1 4: Example 1 feels slightly better in taste than Comparative Example 3: Compared with Example 1 It feels that taste is the same as in Example 1: 2: Feels that Example 1 is slightly inferior in taste compared to Comparative Example 1: 1: Example 1 is more tasteful than Comparative Example 1 I feel inferior.

  The obtained results are shown in Tables 2 and 3. From this result, when the mouthwash was washed with the mouthwash of Example 1, plaque adhesion was clearly reduced as compared with the mouthwash of Comparative Example 1, and it was found that Was confirmed to have an effect of inhibiting the formation of plaque. In addition, it was found that the mouthwash of Example 1 was able to realize a plaque formation inhibitory effect for the user, and also had good taste, and was also excellent in terms of usability.

Example 3 Tablet
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Polydextrose 7
Sugar ester 2
Fragrance 1
Xylitol 15
Palatinose remaining
Total 100

Example 4 Sugar-coated tablets Sugar-coated tablets were prepared using tablets and sugar-coated tablets having the following composition.
Tablet (% by weight)
Oenothera plant extract obtained in Production Example 1.5
Sugar ester 1
Guam gum 0.2
Aspartame 0.01
Palatinose remaining
Perfume
Total 100
Sugar coating (wt%)
Calcium phosphate 1
Aspartame 0.01
Gum arabic 0.5
Carbana wax 0.1
Shellac 0.2
Multi-tose remaining
Fragrance 0.4
Total 100

Example 6 Toothpaste
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Calcium phosphate 30
Glycerin 10
Sorbitol 20
Sodium carboxymethylcellulose 1
Sodium laurate 1.5
Carrageenan 0.5
Saccharin sodium 0.1
Sodium benzoate 0.3
Fragrance 1
Water balance
Total 100

Example 7 Toothpaste
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Sodium laurate 0.8
Lauric acid diethanolamide 1
Propylene glycol 3
60% by weight sorbit solution 35
Methylparaben 0.2
Butylparaben 0.01
Saccharin sodium 0.15
Gelatin 0.3
Sodium alginate 0.9
Aluminum hydroxide 45
Sodium monofluorate 0.76
Fragrance remainder
Total 100

Example 8 mouthwash
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Ethanol 10
Glycerin 5
Citric acid 0.01
Sodium citrate 0.1
Polyoxyethylene hydrogenated castor oil 0.5
Methyl paraoxybenzoate 0.1
Fragrance 0.2
Water balance
Total 100

Example 9 mouthwash
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Sodium laurate 0.8
Lauric acid diethanolamide 0.8
Glycerin 12
Saccharin sodium 0.2
Peppermint oil 0.8
Arginine 0.1
Disodium hydrogen phosphate 0.5
Dipotassium hydrogen phosphate 0.08
Water balance
Total 100

Example 10 Lozenges
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Maltitol 21
Gum arabic 1.5
Sucrose fatty acid ester 2.5
Citric acid 4
Powder flavor 1
Xylitol balance
Total 100

Example 11 Oral Pasta
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Liquid paraffin 13
Cetanol 10
Glycerin 25
Sorbitan monopalmitate 0.6
Polyoxyethylene sorbitan monostearate 5
Sodium lauryl sulfate 0.1
Benzotonium chloride 0.1
Methyl salicylate 0.1
Saccharin 0.2
Fragrance 0.25
Water balance
Total 100

Example 12 mouthwash
(weight%)
Oenothera plant extract obtained in Production Example 1 1
Grape genus plant extract obtained in Reference Production Example 1 1
Ethanol 10
Glycerin 5
Citric acid 0.01
Sodium citrate 0.1
Polyoxyethylene hydrogenated castor oil 0.5
Methyl paraoxybenzoate 0.1
Fragrance 0.2
Water balance
Total 100

Example 13 mouthwash
(weight%)
Oenothera plant extract obtained in Production Example 1 0.5
Concentrated glycerin 5
Propyl paraoxybenzoate 0.02
Polyoxyethylene hydrogenated castor oil 2
Fragrance 0.1
Ethanol 5
Purified water balance
Total 100

It is a figure which shows the strength of the inhibitory action which an evening primrose plant extract has with respect to the result of Test Example 1, ie, GTase derived from Streptococcus sobrinus . It is a figure which shows the strength of the inhibitory action which an evening primrose plant extract has with respect to the result of Test Example 2, ie, GTase derived from Streptococcus sobrinus . It is a figure which shows the strength of the inhibitory action which an evening primrose genus plant extract has with respect to the result of Test Example 3, ie, GTase derived from Streptococcus mutans . It is a figure which shows an example of the criterion of the adhesion state of the plaque in Test Example 4, ie, the representative example about the relationship between each plaque score and the adhesion state (stained area) of plaque.

Claims (6)

A method for producing an oral composition for oral cavity prevention, comprising: a step of defatting a red pine genus plant, extracting with a polar solvent, and a step of blending the extract obtained in the step .   The manufacturing method according to claim 1, wherein the composition for oral cavity for dental caries contains 0.00001 to 20% by weight of the extract.   The production method according to claim 1 or 2, wherein the extract is an extract of seeds of the genus Oleaceae.   The production method according to any one of claims 1 to 3, wherein the plant of the genus Rubiaceae is the evening primrose. A method for producing a GTase inhibitor, comprising: a step of defatting a red pine genus plant, followed by a step of extracting with a polar solvent, and a step of blending the extract obtained in the step .   The manufacturing method according to claim 5, wherein the extract is an extract of seeds of the genus Oleaceae.