JP2001278798A - Cariostatic agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cariostatic agent comprising a substance safe for human bodies and available at a low cost as an active ingredient and a cariostatic agent comprising a substance capable of being not only safely formulated in a composition for the oral cavity such as a mouthwash but also formulated as a food even in a food and drink as an active ingredient. SOLUTION: This cariostatic agent comprises flesh or a fruit juice obtained from fruits of a melon, a mandarin, a valencia orange, a banana and Citrus sudachi hort. ex Shirai as the active ingredient. The composition for the oral cavity contains the above cariostatic agent.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an anti-carious agent. More specifically, the present invention relates to an anti-carious agent containing juice obtained by being squeezed or extracted from a specific fruit as an active ingredient.

[0002]

2. Description of the Related Art Caries are generally called caries, and the causes thereof are considered as follows. Naturally, certain kinds of bacteria are adsorbed on teeth due to the interaction between the glycoprotein, which is a component in saliva, and the cell surface substance of bacteria resident in the oral cavity. This bacterium is a glucosyltransferase (hereinafter referred to as "G"), which is an enzyme secreted outside the cells.
Tase ”. ), Food-derived sucrose is denatured into a sticky polysaccharide, and adheres more firmly to the tooth surface. This is generally called plaque, and the attached bacteria metabolize sugars in the plaque and generate an acid as a metabolite thereof.
The acid demineralizes the enamel surface of the tooth and promotes dental caries. It is said that plaque causes not only caries, but also bad breath, and depending on its progress, periodontal disease, gingivitis, and even alveolar pyorrhea.

[0003]

An object of the present invention is to provide an anti-cariogenic agent which is available at a low cost and contains a substance which is safe for the human body as an active ingredient. Further, a second object of the present invention is to provide an anti-cariogenic agent comprising, as an active ingredient, a substance which can be safely compounded in oral compositions such as mouthwashes and dentifrices, and which can also be compounded in foods and drinks. It is to be.
A third object of the present invention is to provide an anti-cariogenic agent which is useful for preventing oral diseases such as bad breath, periodontal disease, gingivitis and alveolar pyorrhea by suppressing the formation of plaque. It is.

[0004]

SUMMARY OF THE INVENTION In view of the above circumstances, the present inventor has conducted intensive studies day and night in search of an anti-cariogenic agent which is suitable for prevention of dental caries and periodontal disease and which is safe for the human body. However, the melon, mandarin, Valencia orange, banana or citrus fruit or juice obtained by squeezing or extracting fruit has the effect of significantly preventing S. mutans and S. sobrinus from adhering to teeth. Finding something,
It was confirmed that it was effective as an anti-carious agent. The present invention
It has been completed based on such knowledge.

That is, the present invention relates to melon, mandarin,
It is an anti-cariogenic agent containing as an active ingredient the pulp or juice of fruits such as Valencia orange, banana or Sudachi.

The present invention is also an oral composition (including food and drink) containing the above-mentioned anti-carious agent.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The flesh or juice of melon, mandarin, Valencia orange, banana or banana used as an active ingredient of the anti-carious agent of the present invention crushes the fruit of melon, mandarin, Valencia orange, banana or banana, respectively. , Pulp, juice or extract that can be obtained by pressing or extracting. Here, the fruit does not matter whether the pulp or the skin is used, and any of the fruit including the pulp and the rind, the pulp excluding the rind, or the rind can be used as a raw material.

[0008] The method of squeezing is not particularly limited.
Raw fruit as it is, or while adding an antioxidant such as vitamin C and erythorbic acid, obtain squeezed fruit juice by crushing or squeezing, preferably pectin-degrading enzyme treatment, and then, if necessary, centrifugation or A method of removing solid components such as pulp by a separation means such as filtration to obtain a juice can be given. Also, as the extraction method,
Although not particularly limited, the raw fruit is mixed with an organic solvent such as alcohol (ethanol or the like), crushed, and immersed and pressed for extraction, or, if necessary, while heating and refluxing, and then extracting the organic solvent. After evaporation, centrifuge or organic solvent (hexane, chloroform, etc.)
And subjecting the mixture to a fractionation step to obtain an extract. The obtained squeezed juice or extract can be used as it is, but it is further subjected to a concentration step as concentrated fruit juice, puree or paste, or to a drying step such as spray drying, reduced pressure drying or freeze drying. Can be used as dried fruit juice. In addition, these fruit juices are all sold commercially as foods or food ingredients, and they can be used simply.

The anti-cariogenic agent of the present invention contains the above-mentioned melon, mandarin, Valencia orange, banana or succulent flesh or juice as an active ingredient, and is used for various oral medicines such as foods, quasi-drugs, and pharmaceuticals. By being blended as a component of the composition, it can be effectively used to prevent plaque formation in the oral cavity. The anti-cariogenic agent of the present invention only needs to contain an effective amount of the fruit juice component of the fruit, and as long as it does not limit inclusion of an insoluble solid component such as a pulp component or a pulp component.

[0010] Oral compositions include both those which are taken orally, such as food and drink, and those used in the mouth, such as toothpaste and mouthwash. Specific examples of the oral composition include various foods such as troches, chewing gum, candy, gummy candy, chocolate, juice, and the like; dentifrice (paste,
Liquid, powder solid), mouth refresher such as mouth spray, chewing agent, troche, oral pasta, gargle,
Pharmaceutical or quasi-drugs such as syrups; and oral cosmetics such as dentifrices, mouthwashes and mouth rinses. Preferably, foods such as troches, chewing gums and candy, and mouthwashes such as dentifrices, mouthwashes and mouth rinses can be mentioned. These forms and dosage forms are not particularly limited, and can be arbitrarily determined according to the type.

The application concentration of the anti-caries agent of the present invention can be appropriately selected and determined in consideration of various factors such as the type of the oral composition, the ratio of the active ingredient contained in the fruit juice, and dilution in the mouth. As a ratio of various fruit juice (pulp) components blended in 100% by weight of the oral composition, specifically, melon juice (100% squeezed juice) contains 0.1% by weight or more, particularly 5% by weight or more of mandarin Fruit juice (100% juice) is 0.1
% Or more, especially 3% or more, Valencia orange juice (100% juice) is 0.1% or more, especially 5% or more, and banana pulp (juice) (100% juice) is 0.1% or more.
1% by weight or more, especially 1% by weight or more, and further, citrus fruit juice (vinegar) (100% squeezed juice) can be 0.1% by weight or more, especially 5% by weight or more.

The anti-cariogenic agent of the present invention is known or may be known in the future as an antibacterial agent against S. mutans or S. sobrinus , as long as the effect of the present invention is not impaired. Anti-adhesion agent to surface or anti-GTase
It can also be compounded in combination with components such as an anti-caries agent or an anti-inflammatory agent.

The anti-caries agent of the present invention can be used in combination with a component usually contained in the oral composition according to the type of the oral composition to be applied.

For example, in the case of toothpaste, an abrasive such as dibasic calcium phosphate, calcium carbonate, insoluble sodium metaphosphate, amorphous silica, aluminum oxide, etc .; carboxymethyl cellulose, hydroxyethyl cellulose, alginate, carrageenan, gum arabic, polyvinyl alcohol, etc. Binders such as polyethylene glycol, sorbitol, glycerin, propylene glycol;
Sodium lauryl sulfate, sodium dodecylbenzene sulfonate, hydrogenated coconut fatty acid sodium monoglyceride monosulfate, sodium lauryl sulfoacetate,
Foaming agents such as sodium N-lauroyl sarcosinate, N-acyl glutamate, sucrose fatty acid esters and the like can be mentioned. Further, flavoring agents such as menthol and flavoring or sweetening agents, preservatives and the like which are usually used therefor can be used. Can be blended. In the case of mouthwashes such as mouthwashes and foods such as chewing gum, conventional ingredients can be used in combination. In addition, as a sweetener combined and used, those with low or no caries are preferable, and for example, D-xylose, xylitol, saccharin sodium, aspartame, trehalose and the like can be suitably mentioned.

Further, the anti-caries agent of the present invention comprises lysozyme chloride, lytic enzyme, dextranase, mutanase, chlorhexidine, sorbic acid, alexidine, hinokitiol, cetylpyridinium, alkylglycine, alkyldiaminoethylglycine salt, monofluorophosphate Sodium, sodium fluoride, stannous fluoride, water-soluble primary or secondary phosphate, quaternary ammonium compound,
It can be used in combination with components such as sodium chloride.

[0016]

The present invention will be described in more detail with reference to the following Test Examples and Examples, which should not be construed as limiting the present invention. <Test Example> Test Example 1 Melon Juice Plaque (bacteria) described below shows the anti-cariogenic effect of melon juice.
It was evaluated by an adhesion suppression test. The plaque (bacteria) adhesion test was performed using a glass test tube instead of the tooth surface. (i) Streptococcus mutans plaque against bacteria (bacteria) adhesion prevention effect First, Streptococcus mutans bacteria MT8148 strain 1% overnight culture 30μl of brain heart infusion liquid medium containing (serotype c) (BHI medium) sucrose 3 ml of a BHI medium containing sugar (test tube with a sterilized lid) was added thereto, and 5-fold concentrated melon juice was mixed at various concentration ratios. The test tube was tilted to 30 ° and incubated at 37 ° C. for 18 hours. Stationary culture was performed.

The melon juice was prepared as follows. First, the melon pulp is crushed and pressed to collect the juice, then the solid content is separated and removed by centrifugation, and the obtained juice is sterilized at 100 ° C. for 50 seconds, and then cooled, and the juice concentration is about 5 times. To obtain a cloudy 5-fold concentrated melon juice.

After culturing for 18 hours, the test tube was slowly rotated three times while keeping the angle of 30 °, and the culture solution was slowly decanted into an empty test tube (sample 1). 3 ml of the above-mentioned 1% sucrose-containing BHI medium is placed in an empty test tube having plaque (fungi) adhered to the inside surface of the test tube, and the mixture is stirred with a vortex mixer to attach plaque (fungus) on the inside surface of the test tube. A part of the kimono was washed and eluted, and the culture solution containing the plaque (bacteria) eluate was decanted into an empty test tube (sample 2).
Then, 3 ml of 1% sucrose-containing BHI medium was again added to the obtained plaque (bacteria) attached test tube, the plaque (bacteria) firmly adhered to the inner surface of the test tube was scraped off with a spatula, and the solution was sampled. It was set to 3. Note that, of the above operations, the vortex stirring operation is based on the assumption that the mouth is rinsed. That is, Sample 2 reflects the amount of plaque (bacterium) that can be obtained by rinsing the mouth, and Sample 3 reflects the amount of plaque (bacterium) that cannot be obtained by rinsing the mouth.

Further, the amount of plaque (bacteria) contained in each sample solution was determined by measuring the turbidity (OD: absorbance at 550 nm) of the solution. Next, the plaque (bacteria) adhesion rate was determined from the turbidity of the solution according to the following formula, and the plaque (bacterium) adhesion suppression effect of melon juice was evaluated based on the adhesion rate (%).

[0020]

(Equation 1)

The results are shown in Table 1. In addition, as a control, the experiment was carried out in the same manner as described above without adding melon juice to the test tube (melon juice concentration: 0 μl / 3 ml), and as a comparative example, peach juice was replaced with melon juice (5-fold concentrated juice). Table 1 also shows the plaque (bacteria) adhesion rate when the same test was performed using 4 times concentrated juice).

[0022]

[Table 1]

[0023] (ii) Streptococcus performed plaque (bacteria) adhering inhibition tests in the same manner as above (i) the plaque (bacteria) adhering inhibition Streptococcus sobrinus bacteria 6715 strain (serotype g) for sobrinus bacteria, melon The plaque (bacteria) adhesion inhibitory effect of the juice was evaluated. Table 2 shows the results.

[0024]

[Table 2]

From the above results, melon juice was found to be plaque (fungus)
It has an antiadhesive effect and was found to be an effective anti-carious agent.

Test Example 2 Mandarin Juice The anti-cariogenic effect of mandarin juice was evaluated in the same manner as in Test Example 1 (i) and (ii) by performing a plaque (fungus) adhesion inhibition test.

About such mandarin juice (i) Strep
plaque (bacteria) adhesion inhibitory action against tococcus mutans bacteria,
And (ii) Table 3 and Table 4 show the results of examining the plaque (fungus) adhesion inhibitory effect on Streptococcus sobrinus bacteria, respectively. The plaque (bacteria) adhesion inhibitory effect on S. mutans bacteria was compared with mandarin juice (6
The results of the same test using peach juice (4-fold concentrate) instead of the 2-fold concentrate are also shown.

[0028]

[Table 3]

[0029]

[Table 4]

From the above results, it was found that mandarin juice has a plaque (bacteria) adhesion inhibitory action and is an effective anti-carious agent.

Test Example 3 Valencia Orange Juice The anti-cariogenic effect of Valencia orange juice was measured using the test example 1 described above.
In the same manner as (i) and (ii), evaluation was performed by performing a plaque (bacteria) adhesion inhibition test.

Valencia Orange Juice (i) Str
Tables 5 and 6 show the results of examining the plaque (bacteria) adhesion inhibitory effect on eptococcus mutans bacteria and (ii) the plaque (bacteria) adhesion inhibition effect on (ii) Streptococcus sobrinus bacteria, respectively. As a comparative example, the plaque (four-fold concentrate) was replaced with peach juice (five-fold concentrate) for the plaque (bacteria) adhesion inhibitory effect on S. mutans bacteria.
The results of a similar test using are also shown.

[0033]

[Table 5]

[0034]

[Table 6]

From the above results, it was found that Valencia orange juice has a plaque (bacteria) adhesion inhibitory action and is an effective anti-carious agent.

Test Example 4 Banana pulp (pure) The anti-cariogenic effect of the banana pulp was determined by examining (i) and (i) in Test Example 1 above.
Evaluation was performed by performing a plaque (bacteria) adhesion inhibition test in the same manner as in i). In addition, squeezing the fruit as banana pulp,
Banana puree prepared by heating and cooling was used.

About such banana juice (i) Streptoco
plaque (fungus) adhesion inhibitory action against ccus mutans bacteria, and
(ii) Plaque (bacteria) against Streptococcus sobrinus bacteria
Tables 7 and 8 show the results of examining the adhesion suppressing effect, respectively. The plaque (fungus) adhesion inhibitory effect on S. mutans bacteria is also shown as a comparative example by using peach juice (4-fold concentrate) instead of banana pulp (pure).

[0038]

[Table 7]

[0039]

[Table 8]

From the above results, banana pulp is plaque (fungi)
It has an antiadhesive effect and was found to be an effective anti-carious agent.

Test Example 5 Sudachi Juice The anti-cariogenic effect of Sudachi juice was determined by comparing (i) and (i) in Test Example 1 above.
Evaluation was performed by performing a plaque (bacteria) adhesion inhibition test in the same manner as in i).

[0042] Specifically, the citrus fruit juice (5-fold concentrate) has the following effects: (i) the action of inhibiting plaque (fungus) adhesion to Streptococcus mutans bacteria, and (ii) the effect of Streptococcus sobrin.
The plaque (bacteria) adhesion-suppressing action against the U.S. bacteria was examined. The results are shown in Tables 9 and 10, respectively. The plaque (bacteria) adhesion inhibitory effect on S. mutans bacteria was compared with the results of a similar test using peach juice (4-fold concentrate) instead of danchi juice (5-fold concentrate) as a comparative example. Shown.

[0043]

[Table 9]

[0044]

[Table 10]

From the above results, the citrus juice is plaque (fungus)
It has an antiadhesive effect and was found to be an effective anti-carious agent.

<Examples> Examples of application of the anti-caries agent of the present invention will be described below as examples. The unit of each prescription means part by weight unless otherwise specified.

Example 1 Chewing gum Calcium carbonate 5.0 Melon juice (5 times concentrated) 2.0 Gum base 30.0 Erythritol 10.0 Xylitol 40.0 Maltitol 12.0 Flavor 1.0 Total 100.0.

[0048]

Example 3 Sugar-Coated Tablet A mouthwash was prepared by coating 200 parts by weight of a tablet portion with 130 parts by weight of a sugar-coated portion.

[0050]

[0051]

[0052]

[0053]

[0054]

[0055]

Example 10 Oral Pasta Liquid Paraffin 13.00 Cetanol 10.00 Glycerin 25.00 Sorbitan Monopalmitate 0.60 Polyoxyethylene Sorbitan Monostearate 5.00 Sodium Lauryl Sulfate 0.10 Methyl Benzotonium Chloride 0.10 Methyl Salicylate 0.10 Saccharin 0.20 Flavor 0.25 Melon Juice (5 times concentrated) ) 2.00 water balance.

A total of 100.00.

In the above Examples 1 to 10, mandarin juice (6-fold concentration) was used instead of melon juice (5-fold concentration),
Alternatively, each formulation was prepared in the same manner using Valencia orange juice (5-fold concentration), banana puree, and Suzuchi juice (5-fold concentration).

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor: Shoko Yoshii 3-13-35, Mitsujinaminami, Yodogawa-ku, Osaka-shi, Osaka F-term (reference) 4C083 AA111 AA112 AA122 AB032 AB222 AB282 AC062 AC102 AC122 AC132 AC232 AC432 AC482 AC582 AC642 AC782 AC862 AD272 AD302 AD352 AD432 CC41 DD22 DD23 EE32 4C088 AB19 AB62 AB71 AC04 MA52 ZA67

Claims (2)

[Claims] 1. An anti-cariogenic agent comprising, as an active ingredient, at least one kind of fruit pulp or juice selected from the group consisting of melon, mandarin, Valencia orange, banana and citrus. 2. An oral composition comprising the anti-cariogenic agent according to claim 1.