KR20150114312A - Oral composition for halitosis suppression - Google Patents

Abstract

The present invention relates to an oral composition which has excellent effects on inhibition of halitosis, dental caries, and periodontal diseases, wherein the oral composition comprises natural medicinal ingredient extracts essentially including a Scutellaria baicalensis extract and a Schizonepeta rhizome extract, and additionally including a forsythia fruit extract, a lonicer aflower extract, and a platycodon extract, etc, and the oral composition further comprises tocopheryl acetate and malic acid. The oral composition comprises 0.1 to 5.0 wt% of a mixture of the natural medicinal ingredient extracts including the Scutellaria baicalensis extract and the Schizonepeta rhizome extract; 0.01 to 1.0 wt% of tocopheryl acetate; and 0.01 to 1.0 wt% of malic acid. The oral composition has an antibacterial activity against Streptococcus mutans which is known as a cariogenic streptococcus, Fusobacterium nucleatum, Porphyromona gingivalis, and Prevotella intermedia which are known as causative bacteria of halitosis, and inhibits activity of MMP3 which is causative bacteria of a periodontal disease, and expression of interleukin-1β and cyclooxygenase-2 (cox-2) which are an index of an anti-inflammation.

Description

Oral composition for halitosis suppression [

The present invention relates to an oral composition for restraining bad breath, and more particularly, it relates to a composition containing a golden extract and a mold extract, And tocopherol acetate and malic acid to further improve oral cavity suppression, dental caries inhibition and periodontal disease inhibitory effect.

It is a disease in which the carbohydrate in the food is metabolized by the microorganisms existing in the tooth surface membrane of the dentate gingiva and the gingival hyphae and the hard tissue of the tooth is destroyed by the action of the locally produced organic acid. It is one of my interests. Although tooth decay is not directly related to human life, it affects the digestive system diseases directly or indirectly because the chewing function of the food is deteriorated. The morbidity rate is especially large for children, and everyone experiences or experiences tooth decay as much as 85% of 5-year-old children. This cavity is caused by the microorganisms in the mouth, especially Streptococcus mutans ( S. mutans ). Streptococcus mutans is a Gram-positive anaerobic bacterium that resides in the bacterial membranes in the oral cavity and decomposes carbohydrates, especially glucose and fructose, contained in foods and releases organic acids, mainly lactic acid, (Kim, Jongbae et al., Public Oral Health and Giftedness, pp. 68-84, 1991).

In most cases, halitosis is believed to be responsible for more than 85% of the oral cavity in the oral cavity, as a result of bacterial contamination of host components and food residues. There are many causes in the oral cavity such as sickle, periodontal disease, impure mouth hygiene, food residue, improper prosthesis, unsanitary denture, mouth cancer. Currently, the most important cause of bad breath is sore throat and periodontal disease. Volatile sulfur compounds (VSC) are the major causative factors of bad breath including hydrogen sulfide (H ₂ S), methyl mercaptan (CH ₃ SH), dimethyl sulfide (CH ₃ SCH ₃ ) , And hydrogen sulfide and methyl mercaptan account for about 90%. These VSCs are mainly caused by the corruption activity of anaerobic bacteria in the mouth using proteins (especially those containing cysteine and methionine), peptides, amino acids, etc. contained in the gingival crevicular fluid, saliva and food residues . Representative Gram-negative anaerobic bacteria include Porphyromonas gingivalis , Prevotella intermedia , Fusobacterium nucleatum , etc., and a large amount of hydrogen sulfide and methyl mercaptan Is being reported. As a result, it has been reported that periodontal disease bacteria play an important role in halothane induction. Many studies have shown that volatile sulfur compounds have deleterious effects on periodontal tissue. VSC effects on tissue include increased mucosal permeability, increased collagen degradation, altered activity of gingival fibroblasts and periodontal ligament cells, And delayed healing were demonstrated experimentally (Kwon, Jin-Hee, et al., Korean Journal of Periodontology 2000).

MMP3, an intracellular matrix protein, is regarded as an important proteolytic enzyme involved in tissue destruction and tissue reconstruction in various diseases such as chronic inflammatory diseases, rheumatoid arthritis, skin diseases, cancer, and the like. Polymorphonuclear leukocytes, , Macrophages, and epithelial cells, and are known to be causative agents of periodontitis (Cho, JH et al., 1999).

Periodontitis is an inflammatory disease that results in the destruction of tooth-supporting tissues such as gingiva, periodontal ligament, cementum and alveolar bone. Periodontitis is strongly associated with the presence of cytokines such as interleukin-1β (IL-1β). Physiologically, activation of cyclooxygenases (COX) is also closely related to periodontitis. In particular, COX-2 is activated by pre-inflammatory cytokines and by products from bacteria (Kim, Sun-Woo et al., 2007).

On the other hand, it is possible to see records using various herbal medicines for preventing or treating oral diseases, and it has been known that various available substances such as honey, rose oil, roasted salt, and herbal medicines have been used for the prevention and treatment of oral diseases However, it is still required to develop an oral composition which is excellent in halitosis suppression, dental caries inhibition and periodontal disease inhibitory effect.

Disclosure of the Invention The present invention has been made in order to solve the problems of the prior art as described above, and as a result, it has been found that a natural medicinal plant extract containing a golden extract and a mold extract as essential components and further containing a combination extract, a Ganoderma lucidum extract, The present invention has been accomplished on the basis of this finding that it is possible to provide an oral composition excellent in halitosis suppression, dental caries inhibition and periodontal disease inhibition by further containing tocopherol acetate and malic acid.

Particularly, it is an object of the present invention to provide an oral composition having a composition which allows the pH to be maintained at preferably 6 to 8, more preferably neutral such as saliva.

The oral composition for suppressing bad breath according to the present invention comprises 0.1-5.0% by weight of a mixture of a natural medicinal plant extract containing a gold extract and a mold extract, 0.01-1.0% by weight of tocopheryl acetate, and 0.01-1.0% by weight of malic acid.

Preferably, the natural medicinal plant extract may further contain 0.1 to 5.0% by weight of an extract selected from the group consisting of allium extract, Gingkohwaseae extract, Gwangyang extract, and a mixture of two or more thereof.

Preferably, the oral composition for restraining bad breath may have a pH within a range of 6 to 8, more preferably neutral.

The oral composition for restraining bad breath may be in a paste form, and in particular, it may be one formulated as a toothpaste, mouthwash or mouthwash.

The oral composition for restraining bad breath may preferably be filled in a double container having a diaphragm.

According to the present invention, there is provided a pharmaceutical composition comprising a natural extract of a natural herbal extract, which comprises a golden extract and a mold extract as essential components, and further comprises a combination extract, a ginger extract, a Gingko extract and the like, and further contains tocopherol acetate and malic acid Thereby providing an oral composition excellent in halitosis suppression, dental caries inhibition and periodontal disease inhibitory effect.

FIG. 1 is a graph showing the inhibitory effect of the oral composition according to the present invention on the expression of interleukin 1? Gene compared with the control group and the comparative example.
FIG. 2 is a graph showing the inhibitory effect of the oral composition according to the present invention on cyclooxygenase-2 (COX-2) gene expression compared with the control group and the comparative example.

Hereinafter, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The oral care composition for suppressing bad breath according to the present invention comprises 0.1 to 5.0% by weight of a mixture of a natural medicinal plant extract containing a golden extract and a mold extract, 0.01 to 1.0% by weight of tocopheryl acetate, and 0.01 to 1.0% do.

The gingival sphincter of the present invention may be extracted by conventional extraction methods well known in the art, preferably by hot water extraction, ethanol, water, and ethane, Can be used.

The gold extract in the natural medicinal plant extract is an extract of roots of gold ( Scutellaria baicalensis GEORGE ), a perennial herbaceous plant belonging to the family Lamiaceae. The herbaceous plant gold is cultivated in various places and widely used for medicinal purposes. The active ingredients are Baicalein, Baicalin, Wogonin, Wogonoside and the like. Taste is good and weakness is cold. Efficacy is a real fire, so the body is a lot of fever, fever and thirst are severely manifested, the severity of consciousness to improve the complicated symptoms. It is known that it has high antipyretic effect from high fever due to cold to high fever of common infectious disease. It shows a remarkable response to relieving heat buildup in the lungs, which is an excellent response to acute pneumonia, high fever of acute bronchitis, and asthma. If digestive disorders are severe and worse with fever in the stomach, it is said to be effective even for acute jaundice. It removes the hyperemia and pain caused by excessive accumulation of heat in the body. It is also used for hypertension, headache, nosebleeds, uterine bleeding, and also has effect on skin eruption boil. This effect is known to be due to relieving symptoms because cold fever causes fever. It relieves the thirst that is caused by the severe heat, and it relieves the fever and sickness every joint of the whole body. In pharmacological experiments, anti - inflammatory reaction, anti - hospital microbial action, antipyretic, diuretic, hypotensive action, hypoglycemic hypoglycemia, stimulation of bile secretion, sedation.

The mold extract is an extract of perennial plant belonging to the family Lamiaceae. The mold is similar in shape and color to the purple lanceolate, which is called as potato, and is mainly used for medicines rather than edible. In one room, the outpost of a dog and Isaac with its flower are used as medicines, which are called "molds". According to the Dongbok-boon primecho, "the nature is warm, it is very tasty and poisonous. It has a bad wind, a strong wind, a lack of sensation in the whole body, a head aches in upper body, a tendon and bones with a sickness, blood, and a fever. Plant in the field. Because it is fragrant and tastes when it is young, it can be eaten as a vegetable. Also, if you make tea for a month, your head and eyes will be cool. It is made with flowers and seeds, and is dried in the sun and used as medicine. My original name is Cassos, because it has a similar smell and taste. " According to the Dongbok Benggang Uni-bang, it is a medicinal medicine that is important for the two winds to heal dizziness in front of the eyes by turning round head and eating " It is good to eat on the month or eat the powder. To treat the breeze, take the same amount of molds and plaster (danggun), and put the powder into the ginger or water with 8g at a time. It is also called aka type estimate. Sweating. In addition, the blood is also treated to drenched in water. The tendon of the hands and feet heals the grounding. It eats on the water in the month. It is also good to eat raw bowls with a single word. A paralytic cures the mouth and eyes of the crooked, jerky, and all winds. It eats on the water in the month. It is effective if you put it on the vinegar and it attaches to the vinegar. It is good to dine deep in water. When the head is sore at the upper limit, 40 grams of mold is darkly eaten. Sweat and untie the table. It eats on the water in the month. It heals what it does not stop. I burn the remaining fragrance and eat it 8g at a time. "

The present invention includes a natural medicinal herb extract which essentially comprises the gold extract and the mold extract, wherein the natural medicinal herb extract is contained in an amount within a range of 0.1 to 5.0% by weight based on the total weight of the oral composition for restraining bad breath according to the present invention Is preferable for the bad breath suppressing effect.

The oral composition for suppressing bad breath according to the present invention further comprises tocopherol acetate and malic acid in addition to the above-mentioned natural medicinal plant extract.

The tocopherol acetate is vitamin E acetate, which is different from free vitamin E, and its molecular formula is C ₃₁ H ₅₂ O ₃ , molecular weight of 472.73, which is a pale yellow viscous liquid. Acetic acid acetate is not affected by oxidizing agents such as air, light, and ultraviolet rays. It does not dissolve in water. It is soluble in acetone, chloroform, and ether, and slightly soluble in alcohol. Tocopherol is a vitamin E oil that not only acts as a natural antioxidant but also has the effect of aging skin and cell regeneration, and is mainly used as a capsule in skin. Especially in teeth, it is known that adding an effective amount of this function is effective for prevention of gingivitis, periodontitis (dental flossing), prevention of periodontal disease and gingival disease. The tocopheryl acetate is preferably used in an amount within a range of 0.01 to 1.0% by weight based on the total weight of the oral composition for restraining bad breath according to the present invention.

Malic Acid is also known as malic acid. It is a colorless needle-like crystal having the formula C ₄ H ₆ O ₅ , one asymmetric carbon atom, and D-, L- and DL-3 optical isomers. Left-turn adult L-malic acid is contained in natural fruits and vegetables such as apples and grapes. It has a molecular weight of 134.09, a specific gravity of 1.595, a melting point of 100 ° C, and a boiling point of 140 ° C. The acidity is stronger than the same acidic Jane citric acid, and it is not only cheaper but also has an elegant and sophisticated high-quality acidity and a long duration of acidity, which is used to make a person's taste more pleasant and to sour a refreshing drink. It is also an acidic agent necessary for the metabolism of carbohydrates in humans. In particular, it is a acidic acid in the oral cavity to reduce and inhibit bad breath and act as a pH regulator. The malic acid is preferably used in an amount within a range of 0.01 to 1.0% by weight based on the total weight of the oral composition for preventing bad breath according to the present invention.

Alternatively, the natural medicinal extract may contain an extract selected from the group consisting of allium extract, gingko ghrell extract, ganoderma extract, and a mixture of two or more thereof in an amount of 0.1-5.0 wt%, based on the total weight of the oral composition for the prevention of bad breath, By weight of the total amount of the composition.

The tangle extract is an extract of fruit of the common foreskin and tarsal plants belonging to the ash branch called the tangle. The mature nature of the alchemy is used and cold, and the alum is known to act on the heart, menopause, and liver. It is also known to have efficacies such as sweating, chewing action, drainage detoxification, antipyretic action, antibacterial, antiinflammatory, fever, headache, gout, seawater, sore throat, vaginal discharge, vomiting suppression, vasodilation and blood flow improvement. In addition, the diuretic action (to urinate well), the strong diuretic action (to strengthen the heart and urinate well), detoxification (to remove the toxins from the heat), the incarceration ) Is known to have the effect.

The gingkohwang extract is a semi-evergreen vine plant with a gingko ginseng, which is an extract of the plant of the acorn tree of the dicotyledonous plant. From the past, it has been known that when treating thirsty, treating boils, tearing the skin, releasing inflammation and aggravation of organs, skin necrosis caused by dysentery or fever, mumps or conjunctivitis, tonsillitis, sore throat, bronchitis , Cystitis, and the like. According to the classical medical books, the nature of the rhizome is cold, it tastes a little sweet, it works on the heart and the menopause, it releases the heat, it releases the poison, it makes the gynecology well, it has excellent effect on various inflammatory diseases, And swelling, swelling is said to fix.

The ginseng extract is an extract of Gakgyeong, or bellflower. The bellflower is a native material throughout Korea, and it is a common material to be recognized as one of the three tricolor herbs that are widely used as bellflower seeds, bellflower herbs, and so on. Old bellflower is better than wild ginseng. " In fact, the excellent alkaline food, bellflower, dietary fiber as well as minerals such as calcium, iron, potassium, vitamin B1, B2, vitamin C and tyrosine, tryptophan and rich beneficial amino acids. Because of these nutrients, the Dongbu feelings are said to have 278 records of the effectiveness of the bellflower. Bellflower is a perennial plant belonging to lanterns. It is also called Gakgyeong, Bungdo, Hwacheon, Gyeongcho, Gogyeong, Lee. It is mainly used as a medicine for roots. It is picked in autumn, peeled and dried in the sun, then peeled off the faucet. Platycodin, a kind of saponin, and platycodigenin are contained in roots. Balloons are commonly known to be used for coughs and phlegm. Especially, in bellflower, saponin having a brittle taste, which is known as a major component of ginseng, is contained about 2 g per 100 g. In the case of triterpenoid saponin, it shows efficacy against respiratory diseases. It is known to increase the secretion of mucus from the respiratory mucosa, and to reduce sputum. Bellflower juice, which is a blend of bellflower and pear, is widely used as a preventative for winter colds. The main ingredient of Yonggak Mountain, which is famous for cough and phlegm, is bellflower. Especially in oriental medicine, it is known that it is effective for drainage, genomes, tonsillitis, Choi yu, Jinhae, purulent boils, expectorant of asthma and pulmonary tuberculosis, and pleurisy. Bellflower has the ability to lower blood pressure and blood sugar. It is said that it is beneficial for people with high blood pressure and diabetes who are overweight. Platycodon is known to help people with hyperlipidemia because it enhances secretion of steroids and galic acid in the body and leads to a decrease in body cholesterol. In recent years, tumor suppressing substances have been isolated from platycodon, and antimutagenic effects have also been confirmed. It has also been found that when a substance extracted from ethyl acetate of bellflower is reacted with liver cancer cells, it has a strong antioxidative effect and protects cells against cancer due to oxidative stress.

In addition, the oral composition for restraining bad breath according to the present invention preferably further comprises citric acid in an amount within a range of 0.01 to 1.0 wt% in addition to the malic acid.

It is preferable that the oral composition for restraining bad breath is used in an amount such that the pH is within a range of 6 to 8, preferably neutral.

In the present invention, the oral composition containing the extract or the compound of the present invention can be used in the form of a paste and a liquid. Preferably, the oral composition for suppressing bad breath is paste, but is not limited thereto.

The oral composition of the present invention can be formulated as a toothpaste, mouthwash or mouthwash. In particular, the dentifrice may contain a composition selected from the group consisting of an abrasive, a binder, a moisturizer, an active ingredient, a foaming agent, a sweetener, and a flavoring agent as a formulating ingredient, and the formulation ingredients for formulation into these dentifrices And can be understood as being well known.

Examples of the abrasive include aluminum hydroxide, silicic anhydride, aluminum silicate, calcium secondary phosphate, secondary hydroxide and anhydride, calcium tertiary phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, magnesium tertiary phosphate, magnesium carbonate, Calcium, polymethyl methacrylate, and other synthetic resins may be used in an amount of about 20 to 90% by weight of the total composition (in the case of a paste toothpaste, it may be in a range of 20 to 60% by weight .

Examples of the viscous solution include carrageenin, various incremental cellulose derivatives, zeolite such as xanthan gum and tragacanth gum, synthetic compounds such as polyvinyl alcohol, sodium polyacrylate, polyacrylic acid / maleic acid copolymer and carboxyvinyl polymer Polymer derivatives and the like, and inorganic binders such as silica and laponite in an amount within a range of about 0.3 to 5% by weight of the total composition.

As the moisturizing agent, one or two or more of sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol and the like as a moisturizing agent may be added in an amount of about 10 to 70% By weight.

In addition, sweeteners such as sodium saccharin, stevioside, aspartame, D-xylose and the like may be used in an amount within a range of 0 to 1%, preferably 0.01 to 0.5% by weight of the total composition .

In addition, anionic surfactants, nonionic surfactants, amphoteric surfactants, and / or fluorine (Pluron) and poloxamer surfactants can be used as the surfactant used as the foaming component in the oral composition of the present invention . Examples of the anionic surfactant include sodium alkylsulfate such as sodium laurylsulfate and sodium myristylsulfate, sodium N-lauroylsarcosinate, sodium N-miristyl sarcosinate, and the like Sodium N-acylsarcosinate, and the like. Examples of the nonionic surfactant include sugar-derived fatty acid esters such as sugar fatty acid esters, maltose fatty acid esters, and lactose fatty acid esters, polyoxyethylene Hardened castor oil, polyoxyethylene higher alcohol ether, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene polypropylene fatty acid ester, and the like. Examples of the amphoteric surfactant include N-alkyldiaminoethylglycine such as N-lauryldiaminoethylglycine and N-myristyldiaminoethylglycine, N-alkyl-N-carboxymethylammonium betaine, 2- -Hydroxyethyl imidazoline betaine sodium and the like can be used. And fluorine-based surfactants and poloxamer-based surfactants. In this case, anionic surfactant is mainly used as a foaming component, and sodium alkylsulfate such as sodium lauryl sulfate is the most widely used. These surfactants are used alone or in admixture of two or more. The amount of the surfactant is generally in the range of 0.01 to 0.5% by weight, more preferably 0.05 to 0.3% by weight of the total composition.

In addition to the above, the oral composition of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts thereof, organic acids, protective colloid thickeners, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the oral compositions of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so important, but is generally selected in the range of 0 to 20 parts by weight per 100 parts by weight of the composition of the present invention.

The mouthwash or mouthwash may also contain suitable formulating ingredients, and the formulation ingredients for formulating the oralwashing detergent or mouthwash may be understood to be well known to those skilled in the art, for example, Water having a liquid state at room temperature, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, and the like, and may further contain other coloring agents, flavoring agents, flavoring agents, sweeteners and the like.

The oral composition for restraining bad breath may be filled in a double container having a diaphragm. When filled in a double container having such a diaphragm, the same composition is provided on both sides based on the diaphragm of the double container, but filling with different colors may be preferable for satisfying the preference of the consumer.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be construed as limiting the scope of the invention.

Example 1 shown in the following Table 1 is an oral composition comprising a natural medicinal herb extract containing gold extract and mold extract, essential tocopherol acetate and malic acid as acetic acid according to the present invention, Example 2 is a natural composition The composition of the present invention is the same as the composition of Example 1, except that it further contains an equivalent amount of Yeast Extract, Ganoderma lucidum Extract, and Gwangyang Extract as an extract. On the other hand, the oral composition of Comparative Example 1 is the same oral composition as in Example 1, except that it does not contain the gold extract, mold extract, tocopheryl acetate and malic acid in Example 1 above.

The compositions and contents of the following tables were precisely weighed and mixed, and then they were made into toothpaste as a general toothpaste manufacturing method.

<Experimental Example 1> Antibacterial test of Streptococcus mutans

The oral compositions of Comparative Example 1 and Examples 1 and 2 were formulated as dentifrices and 2 g of the oral composition formulated with toothpaste was diluted and suspended in 9 g of sterilized phosphate buffer.

Raw material name Comparative Example 1 Example 1 Example 2 Amorphous sorbitol 30 30 30 Polyethylene glycol 300 2 2 2 Carboxymethylcellulose One One One Tocopheryl acetate - 0.1 0.1 Sodium saccharin 0.22 0.22 0.22 Sasa and Mt. - 0.1 0.1 Calcium Phosphate Chloride 0.1 0.1 0.1 Precipitated silica (for abrasives) 30 30 30 Precipitated silica (incremental) 3 3 3 Sodium lauryl sulfate 1.55 1.55 1.55 Sodium fluoride 0.22 0.22 0.22 Golden extract - 0.5 0.5 Mold extract - 0.5 0.5 Almond extract - - 0.5 Gold Euphoria extract - - 0.5 Gwangyang extract - - 0.5 Yellow No. 4 (FDC # 5) 0.002 0.002 0.002 Green No. 3 (FDC # 5) 0.0005 0.0005 0.0005 Toothpaste Flavor 1.05 1.05 1.05 Titanium dioxide 0.1 0.1 0.1 Sodium phosphate monobasic 0.1 0.1 0.1 Sodium tertiary phosphate 0.05 0.05 0.05 Purified water 30.6075 29.4075 27.9075

The diluted suspension was serially diluted 2-fold with BHI (Brain heart infusion) medium and injected into a 48-well plate in an amount of 1 ml each. One colony of Streptococcus mutans (ATCC 25175) in an agar medium was suspended in 20 ml of BHI medium, and then further injected into the diluted 48 well plate. After incubation at 37 ° C for 24 hours, the antibacterial activity Were measured by MIC (minimal inhibition concentration), and the results are shown in Table 2 below.

Kinds Comparative Example 1 Example 1 Example 2 Mg / ml 12.5 6.25 6.25

As a result, according to the present invention, in addition to the oral composition (Example 1) containing natural extracts of gold extracts and mold extracts, tocopherol acetate and malic acid acetate (Example 1), and the yeast extract, Ganoderma lucidum extract and Gakgye extract It was confirmed that the oral composition (Example 2) contained was superior in antibacterial activity to Comparative Example 1 in which it was not.

<Experimental Example 2> Antimicrobial activity test in anaerobic microorganism

As in Experimental Example 1, 2 g of the oral composition of Comparative Examples and Examples were diluted and suspended in 9 g of sterilized phosphate buffer, and the experiment was carried out as follows. The MIC method was performed in the same manner as in Experimental Example 1. However, since the anaerobic microorganisms do not grow in the presence of oxygen, they were cultured in an anaerobic incubator (Sheldon Manufactoring Bac 2-2E) fed with 5% hydrogen (H ₂ ) and 95% nitrogen (N ₂ ) The results are shown in Table 3 below.

The strain (mg / ml) Comparative Example 1 Example 1 Example 2 Peugeot Monas Jinji Balis
ATCC 33277 12.50 6.50 6.50 Priotella Intermedia
ATCC 25611 6.50 6.50 6.50 Fusobacterium Nucleotum
ATCC 25586 6.50 6.50 6.50

As a result, according to the present invention, oral compositions (Examples 1 and 2) containing herbal remedies showed similar antibacterial activity to Comparative Example 1, but they also exhibited antibacterial activity against the periodontal disease-causing ginger It is also expected to be effective in suppressing periodontal inflammation.

<Experimental Example 3> Evaluation of inhibitory activity of MMP-3

(Comparative Example 2 and Examples 3 and 4) were used except for sodium fluoride, sodium lauryl sulfate, flavor, pigment and the like in the oral compositions of Comparative Example 1 and Examples 1 and 2 as shown in Table 4 below The inhibitory activity of MMP-3 was evaluated.

Raw material name Comparative Example 2 Example 3 Example 4 Amorphous sorbitol 30 30 30 Polyethylene glycol 300 2 2 2 Carboxymethylcellulose One One One Tocopheryl acetate - 0.1 0.1 Sodium saccharin 0.22 0.22 0.22 Sasa and Mt. - 0.1 0.1 Calcium Phosphate Chloride 0.1 0.1 0.1 Golden extract 0.5 0.5 Mold extract 0.5 0.5 Almond extract - 0.5 Gold Euphoria extract - 0.5 Gwangyang extract - 0.5 Titanium dioxide 0.1 0.1 0.1 Sodium phosphate monobasic 0.1 0.1 0.1 Sodium tertiary phosphate 0.05 0.05 0.05 Purified water 66.43 65.23 63.73

To examine the inhibitory activity of MMP-3, fibroblasts were cultured and treated with the oral composition as described below. Human Gingival Fibroblast was collected from healthy tissue of a person without periodontal disease and used for the first culture. (GibcoBRL, USA) and 1% antibiotics / antimycotics (100 U / ml penicillin, 25 μg / ml amphotericin) supplemented with 10% FBS (Fetal bovine serum) ) D and 100 μg / ml of streptomycin in Dulbecco's modified Eagle's medium (GibcoBRL, USA) at 37 ° C. in a 5% CO ₂ incubator. Gum fibroblast cultured in the medium was replaced with culture medium containing 1% FBS and cultured for 24 hours. Then, sodium fluorophosphate, sodium laurylsulfate, parahydroxybenzoate and mint flavor B70097 (100 ng / ml) was added for 48 hours (MMP-3) after pretreatment for 30 minutes at a concentration of 200 ppm and 500 ppm using phosphate buffer. After culturing, the culture medium was collected at 1500 rpm / 5 min And centrifuged to obtain only the supernatant. The activity of MMP-3 was measured using an ELISA kit (Amersham Bioscience, UK). The results are shown in Table 7.

MMP-3 activity (ng / ml) Untreated group IL-1? Comparative Example 2
+ IL-1? Example 3
+ IL-1? Example 4
+ IL-1? 200ppm 4.25 17.50 16.36 9.25 8.95 500ppm 4.67 18.10 14.56 4.76 4.50

Based on the fact that the composition of Oriental herb medicine (Example 3) containing the extract of Kangyo and extracts of the mold according to the present invention is more effective than the compound of Comparative Example 2 which is not, the extract of Kangyo . In addition, it was found that the herbal composition (Example 4), which contains the extract of Oriental japonica, mold extract, Gingkohwaseae extract and Gil Gyeonggye extract, had a slightly better inhibitory effect on MMP-3 than that of Example 3, Extracts and synergistic effects.

Experimental Example 4 Expression Inhibition Test (RT-PCR) of Anti-inflammatory Indices Interleukin-1? And Cyclooxygenase-2 (cox-

1 × 10 ⁶ Raw 264.7 cell lines were dispensed in a 100 mm Petri dish and an oral composition except one sodium fluorophosphate, sodium lauryl sulfate, p-hydroxybenzoic acid methylate and mint flavor B70097, which could induce toxicity of the cells in the formulation composition (Invitrogen, CA, USA) was used to remove the cells from the culture medium. The cells were then treated with phosphate buffer solution (200 ppm, 500 ppm) Were homogenized. Five minutes later, the cells were collected, transferred to a centrifuge tube, and mixed with 200 μl of chloroform for 15 seconds, left for 3 minutes, and centrifuged at 14000 rpm for 15 minutes. The supernatant containing the ribonucleic acid was transferred to a new tube and mixed with 500 μl of isopropyl alcohol. After 10 minutes, the supernatant was discarded, and 1 ml of 75% ethanol was added to the precipitate. The supernatant was discarded at 10000 rpm for 5 minutes. The supernatant was discarded and the precipitated ribonucleic acid was dried at room temperature for 20 minutes. The dried ribonucleic acid was suspended in distilled water treated with DEPC (Diethylpyrocarbonate, Sigma). After quantification of ribonucleic acid, complementary nucleic acid (cDNA) was synthesized using RT-PreMix (AccuPower RT PreMix, Bioneer). Interleukin-1? And cyclooxygenase-2 primers were mixed with the synthesized cDNA as a template, and the degree of expression of ribonucleic acid was confirmed using PCR Premix (AccuPower PCR PreMix, Bioneer). The synthesized cDNA was mixed with the COX-2 primer as a template, and the degree of expression of ribonucleic acid was confirmed using PCR Premix (AccuPower PCR PreMix, Bioneer). Quantitative analysis was carried out using a densitometer (Image Gauge V 3.46, Koshin Graphic Systems, Fuji Photo Film Co., Japan).

As a result, as shown in Fig. 1 and Fig. 2, the relative inhibition of expression of LPS-treated group was examined. As a result, it was confirmed that the compositions (Examples 3 and 4 ) Effectively inhibited the expression of interleukin-1? And cyclooxygenase-2 gene in Comparative Example 2. In particular, it was confirmed that the expression inhibition rate of cyclooxygenase-2 was remarkably decreased by 50% or more at 500 ppm of Examples 3 and 4. From these results, it is judged that the inhibition rate of the oral composition (Examples 3 and 4) containing the oriental medicine according to the present invention is excellent. From this, it can be confirmed that the oral composition prescribed according to the present invention is highly effective for inhibiting periodontal inflammation.

The oral composition of Example 1 of the present invention including a prescription for oriental medicine, which effectively inhibits growth of dental caries, bad breath, and periodontitis causing bacteria, is effective against MMP-3, which is directly involved in periodontal tissue destruction, and interleukin- Lt; RTI ID = 0.0 &gt; ShigaNase-2 &lt; / RTI &gt; gene expression.

The present invention can be used in the industry for producing oral compositions such as toothpastes and oral cleansers, etc.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

(Without reference numerals)

Claims (7)

Wherein the composition comprises 0.1 to 5.0% by weight of a mixture of a natural medicinal extract comprising a golden extract and a mold extract, 0.01 to 1.0% by weight of tocopheryl acetate, and 0.01 to 1.0% by weight of malic acid. The method according to claim 1,
Wherein the natural medicinal extract further comprises from 0.1 to 5.0% by weight of an extract selected from the group consisting of Yeast Extract, Gingkohyeol Extract, Gakgye Extract, and a mixture of two or more thereof. The method according to claim 1,
Wherein the oral composition for restraining bad breath is in a range of pH within a range of 6 to 8. The method of claim 3,
Wherein the oral composition for halitosis suppression is neutral. The method according to claim 1,
Wherein the oral composition for restraining bad breath is a pasty composition. The method according to claim 1,
Wherein the oral composition for restraining bad breath is formulated into a toothpaste, mouthwash, mouthwash or mouthwash. The method according to claim 1,
Wherein the oral composition for halitosis suppression is filled in a double container having a diaphragm.

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