WO2015013731A1 - Membrane de capteur pour la détection réversible d'analytes - Google Patents

Membrane de capteur pour la détection réversible d'analytes Download PDF

Info

Publication number
WO2015013731A1
WO2015013731A1 PCT/AT2014/000144 AT2014000144W WO2015013731A1 WO 2015013731 A1 WO2015013731 A1 WO 2015013731A1 AT 2014000144 W AT2014000144 W AT 2014000144W WO 2015013731 A1 WO2015013731 A1 WO 2015013731A1
Authority
WO
WIPO (PCT)
Prior art keywords
dye
indicator
indicator dye
sensor membrane
sensor
Prior art date
Application number
PCT/AT2014/000144
Other languages
German (de)
English (en)
Inventor
Gerhard Mohr
Original Assignee
Joanneum Research Forschungsgesellschaft Mbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joanneum Research Forschungsgesellschaft Mbh filed Critical Joanneum Research Forschungsgesellschaft Mbh
Publication of WO2015013731A1 publication Critical patent/WO2015013731A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N2021/758—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated using reversible reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G01N31/221—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for investigating pH value

Definitions

  • the present invention relates to a sensor membrane for the reversible detection of analytes comprising a polymeric support membrane, an indicator dye covalently bound to the support membrane, and optionally a cover layer.
  • Sensor membranes which have a covalently fixed indicator dye for detecting analytes on a carrier membrane have been known for some time and are used for a very wide variety of purposes depending on the bound indicator tartar dyes.
  • Indicator dyes are substances that indicate changes in state in chemical systems through a color change.
  • the conventional absorption indicators for example, pH indicators and redox indicators
  • fluorescence indicators for example, fluorescence indicators.
  • the measuring principle is based on a color change of the indicator dye, for example at a specific pH or a specific redox potential.
  • indicator dyes which either have a single color change or, such as methylene blue, also show a plurality of color changes depending on the pH.
  • a disadvantage of these known indicators is that the color change often takes place either between two poorly distinguishable colors, or that a relatively slow transition takes place from one color to another, such as Bromthymolblau, in which an envelope from yellow to green to takes place blue, so that it is not or very difficult to see, especially with the naked eye, when exactly the change between the two colors has taken place.
  • WO 03/036293 A1 discloses an optical method by means of which it is possible to determine the pH of a solution and its content of dissolved oxygen. It is based on the use of two optical indicators in only one sensor matrix, wherein the two indicators provide two differentiable optical signals, which can be assigned to the respective measured variables.
  • DE 10 2007 053 664 A1 discloses optical sensors for detecting ions, gases and biomolecules, comprising a matrix with one or more indicator substances contained therein, in which one or more indicator substances are present in at least one thin layer of ionic liquid Matrix are included.
  • DE 1603454 A discloses a fiber material which is dyed on the one hand with an indicator dye which is covalently bonded to the fiber material and which indicator should be moved to a color change with materials commonly used in the household such as lemon juice. According to DE 1603454 A, such materials are to be used, for example, in the toy industry in order to make the game interesting for a long time by achieving a color change. In such an indicator provided with color material is therefore completely irrelevant whether and how far the color change takes place within a range or exactly at one point, as long as the color change is reversible.
  • US Pat. No. 6,531,322 B1 discloses an optically evaluable blood glucose test strip in which an indicator dye or a mixture of indicator dyes is integrated on a test strip consisting of two membranes and an inert dye is incorporated in another layer in order to obtain a mixed color from which, in any case, a color shows a pH change, to allow a more accurate determination of the pH.
  • a disadvantage of this patent is the fact that both dyes are only physically integrated and thus can easily be washed out of the membrane, so that the reversibility or multiple use of such a sensor membrane appears only to a very limited extent possible.
  • the present invention now aims to provide a sensor membrane which, even after a plurality of uses, exhibits consistent color changes and color intensities during the color change and, in particular, provides a sensor membrane which exhibits a precise color change, which only occurs over one extends very narrow range of values, in order to make an exact measurement possible in this way.
  • the sensor membrane according to the invention is essentially characterized in that on the support membrane, another one of the indicator dye having different color dye is covalently fixed and that the further dye and the indicator dye are covalently bound to, in particular, different nanoparticles or microparticles consisting of cellulose, polyurethane hydrogel, poly (hydroxyethyl methacrylate), amino-polymer, polyacrylates, silicones, epoxides, sol-gel glasses or polyolefins, and then into a polymer support membrane embedded or physically or chemically coupled.
  • the support membrane By further covalently fixing a dye having a different color from the indicator dye to the support membrane, it is possible to provide a sensor membrane which is available for a plurality of measurements without either the indicator dye or the second sensor membrane Dye are washed out. Furthermore, by covalent fixing of both the indicator dye and the second dye, it is possible to provide a sensor element which has a reversible optical property in order to be able to continuously monitor, for example, a reaction proceeding over a long period of time or a chemical compound .
  • the optical property of the sensor element, to which the indicator dye and the second dye are covalently fixed can also be formed irreversibly, with which, for example, exceeding a limit value of a component to be monitored can be reliably detected.
  • the indicator dyes and other dyes are covalently bonded or copolymerized to particular different nano and microparticles consisting of cellulose, polyurethane hydrogel, poly (hydroxyethyl methacrylate), amino, polyacrylates, silicones, epoxies, sol-gel glasses or polyolefins can Any sensor membranes or sensor elements are produced, wherein the micro- and nanoparticles are physically or chemically coupled to the polymeric support membrane.
  • Indicator dyes and further dyes can be covalently bound to the same or to different nano- and microparticles.
  • the further colorant and the indicator dye are fixed to mutually different layers of the polymeric support membrane.
  • the sensor element is substantially designed so that the further dye with the indicator dye results in a clear color contrast in a color change of the indicator dye having mixed color.
  • the further dye with the indicator dye results in a mixed color which allows a clear color contrast in a color change of the indicator dye, it is possible to determine the envelope and thus the measured optical property of the monitored component or the substance to be monitored significantly more accurate than with an indicator dye alone.
  • an indicator dye alone the turning point with the naked eye can often not be clearly recognized, since until the actual color change a slow or continuous color change takes place, which is often very poorly recognized or distinguished by the naked eye.
  • the indicator dye is a pH indicator dye or a redox indicator dye or other selective indicator dye.
  • Both pH indicator dyes and redox indicator dyes can be used to track a wide variety of chemical reactions and thus for a wide variety of uses.
  • selective indicator dyes for example, oxygen, reactive oxygen species, such as hydrogen peroxide, CO2, amines, carboxylic acids, ketones, Na +, K +, Ca 2 +, Mg 2 +, ions, saccharides, alcohols, diols, thiols, nitrogen oxides, proteins, metabolites, organophosphorus Compounds and formic acid are investigated or these compounds and ions are traced.
  • the indicator dye is, for example, an indicator dye turning from yellow to red, in particular 2 - ((4- (2-hydroxyethylsulfonyl) phenyl) diazenyl) -4-methoxyphenol, 4-fluoro-2 - ((4 - (2-hydroxyethylsulfonyl) phenyl) dia- zenyl) phenol or 4-bromo-2 - ((4- (2-hydroxyethylsulfonyl) cyclohexa-1,4-dienyl) diazenyl) phenol.
  • the indicator dye is, for example, an indicator dye turning from yellow to red, in particular 2 - ((4- (2-hydroxyethylsulfonyl) phenyl) diazenyl) -4-methoxyphenol, 4-fluoro-2 - ((4 - (2-hydroxyethylsulfonyl) phenyl) dia- zenyl) phenol or 4-
  • an indicator dye turning from, for example, yellow to red and adding an inert dye which has a darker base color, it is possible to create a clear color change, so that the optical property in the form of a color change of the indicator element can be reliably perceived and, in particular, the smallest changes are perceived can be.
  • the indicator dye for example, 1-hydroxy-4- [4- (2-hydroxyethylsulfonyl) phenylazo] naphthale ne-2-sulfonic acid potassium salt (Chromoionophore XVI I), dilithium (1 +) ion 10-amino-3- (vinylsulfonyl) -2,4-dioxo-3-azatricyclo [7.3.1.0 A ⁇ 5, 13 ⁇ ] trideca-1 ( 13), 5,7,9, 11-pentaene-7,11-disulfonate (Lucifer Yellow VS dilithium salt), 4-hydroxy-3 - ((4- (2-hydroxyethylsulfonyl) phenyl) diazenyl) benzonitrile, 4- (trifluoroacetyl) -4 '- [N- (1-methacryloxyunde
  • the indicator dye for example, 1-hydroxy-4- [4- (2-hydroxyethylsulfonyl)
  • the further dye for example, 2- (3- (4-amino-9, 10-dihydro-3-sulfo-9, 10-dioxoanthracen-4-yl) aminobenzenesulfonyl ) vinyl) disodium sulfate, trade name Reactive Blue 19, trisodium 5-acetamido-4-hydroxy-3- (2- ⁇ 2-hydroxy-5- [2- (sulfonatooxy) ethanesulfonyl] phenyl ⁇ diazene-1 - yl) - naphthalenes-2-disulfonate, trade name Remazol Brilliant Violet 5R or tetrasodium (3Z) -5-amino-4-oxo-6- [4- (2-sulfonato-oxyethylsulfonyl) -phenyl] diazenyl-3 - [[4 (2-sulfonato-oxyethylsulfonyls) -phenyl]
  • the sensor membrane is essentially characterized in that the further dye and the indicator dye in a ratio of 1: 20 to 1: 1 are used. By choosing the ratio of the further dye and the indicator dye, it is possible to adjust the color depth and thus to maximize the color depth. adjust possible contrast of the two colors of the indicator dye before and after the turnover by adding another dye.
  • the invention is preferably developed in such a way that the polymeric carrier membrane is formed from a colorless, transparent polymer.
  • the polymeric carrier membrane is formed from a colorless, transparent polymer.
  • a polymeric support membrane for example cellulose membranes, polyurethane hydrogels, poly (hydroxyethyl methacrylates), amino polymers, polyacrylates, silicones, epoxies, sol-gel glasses or polyolefins are used, the parameter of the membrane and by selecting the porosity, the diffusion properties and the layer thickness of the membrane, in particular the response speed of the sensor membrane are controlled and thus a fine adjustment of the measuring range can be achieved.
  • the polymeric carrier membrane is formed multi-layered, any sensor membranes or sensor elements can be produced.
  • a support membrane layer may constitute a rigid layer, which is provided to protect the sensitive sensor membrane and the actual sensor membrane then applied only on this sensor, or it may be arranged one above the other several layers of the support membrane, of which further dyes and the Indicator dye are arranged only in one layer.
  • the invention is developed so that the polymeric support membrane is covered with a porous, transparent cover layer at least on one side having the covalently bound indicator dye ,
  • a porous, transparent cover layer on the sensor membrane or support membrane containing the indicator dye, it is possible, on the one hand, to prevent damage to the sensor membrane and, on the other hand, to ensure that For example, in the case of a redox indicator, the redox potentials can be reliably and reliably detected and detected despite the presence of a cover layer.
  • Example 1 2- (3- (4-Amino-9,10-dihydro-3-sulfo-9,10-dioxoanthracen-4-yl) aminobenzenesulfonyl) vinyl) disodium sulfate in combination with 1-hydroxy 4- [4- (2-hydroxyethylsulfonyl) phenylazo] naphthalene-2-sulfonic acid potassium salt at a dye ratio of 1: 5 a color change from olive green to violet:
  • Example 2 and FIG. 1 Tetrasodium (3Z) -5-amino-4-oxo-6- [4- (2-sulfonato-oxyethylsulfonyl) -phenyl] diazenyl-3 - [[4- (2-sulfonatooxyethylsulfonyl) -phenyl] -hydrazinylidenes] -naphthalenes-2,7-disulfonates in combination with 2 - ((4- (2-hydroxyethylsulfonyl) phenyl) diazenyl) -4-methoxyphenol at a dye ratio of 1:10 a color change from yellow-green to deep red wherein Figure 1 shows both the indicator set alone and the covalently bound indicator and further dye fixedly shown;
  • Example 3 2- (3- (4-Amino-9,10-dihydro-3-sulfo-9,10-dioxoanthracen-4-yl) aminobenzenesulfonyl) vinyl disodium sulphate in combination with 4-fluoro 2 - ((4- (2-hydroxyethylsulfonyl) phenyl) diazenyl) phenol at a dye ratio of 1:10 a change in color from green to deep red.
  • 100 mg of the indicator dye are thoroughly mixed with 1 g of concentrated sulfuric acid in a mortar and allowed to stand for 30 minutes in a desiccator. Thereby, the 2-hydroxyethylsulfonyl group of the indicator dye could be converted into a reactive sulfonate.
  • This mixture is then emptied into 900 ml of water and neutralized with 1 .6 ml of 32% sodium hydroxide solution. Thereafter, 25.0 g of sodium carbonate in 100 ml of water and subsequently 5.3 ml of a 32% sodium hydroxide solution are added. At the same time, a solution of 20 mg of the further dye in 100 ml of water is added. The polymer membranes are placed in this staining solution.
  • the dye sulfonate is converted into a chemically reactive vinyl sulfonyl derivative which covalently binds to the reactive groups of the poly- meres (for example, hydroxyl groups of cellulose or amino groups of the polyurethane) binds.
  • the additional dye thus binds to the reactive groups of the polymer.
  • Fig. 1 is an example of an indistinct color change of a pure covalent bound to a polymer membrane indicator dye compared to a significant color change in a mixture of indicator dye and further dye covalently bonded to a polymer membrane.
  • the color change of the pure indicator dye is shown with a color change from orange to red and in the lower series the color change of an indicator covalently attached to the support and a further dye covalently bonded to the support, with a color change from green to red ,

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

L'invention concerne une membrane de capteur destinée à la détection réversible d'analytes, comprenant une membrane de support polymère, un colorant indicateur lié de façon covalente à la membrane de support et éventuellement une couche de revêtement. Un autre colorant, de couleur différente de celle du colorant indicateur, est fixée de manière covalente à la membrane de support et le colorant indicateur et l'autre colorant sont liés de façon covalente à des nanoparticules ou des microparticules, notamment différentes les unes des autres et constituées de cellulose, d'hydrogel de polyuréthane, de polyhydroxyéthylméthacrylate, d'aminopolymère, de polyacrylates, de silicones, de résines époxydiques, de verres sol-gel, ou de polyoléfines, et sont ensuite incorporées dans une membrane de support polymère ou couplées physiquement ou chimiquement.
PCT/AT2014/000144 2013-07-31 2014-07-21 Membrane de capteur pour la détection réversible d'analytes WO2015013731A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA613/2013 2013-07-31
ATA613/2013A AT514611B1 (de) 2013-07-31 2013-07-31 Sensormembran zur reversiblen Detektion von Analyten

Publications (1)

Publication Number Publication Date
WO2015013731A1 true WO2015013731A1 (fr) 2015-02-05

Family

ID=51428984

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2014/000144 WO2015013731A1 (fr) 2013-07-31 2014-07-21 Membrane de capteur pour la détection réversible d'analytes

Country Status (2)

Country Link
AT (1) AT514611B1 (fr)
WO (1) WO2015013731A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019051205A1 (fr) * 2017-09-07 2019-03-14 Portland State University Capteurs à hydrogel permettant la détection d'ions métalliques
CN114460000A (zh) * 2020-11-10 2022-05-10 恩德莱斯和豪瑟尔分析仪表两合公司 用于确定溶液的pH值的指示剂

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111443007B (zh) * 2020-04-13 2022-08-05 厦门眼科中心有限公司 一种基于水凝胶复合膜流速测定透明质酸酶浓度的检测方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1603454A1 (de) 1965-11-24 1970-10-22 Mattel Inc Fasermaterial
US5853669A (en) 1991-09-30 1998-12-29 Merck Patent Gesellschaft Mit Beschrankter Haftung Sensor membrane for indicating the PH of a sample, the fabrication and use thereof
WO2002035228A2 (fr) * 2000-10-27 2002-05-02 Attomol Gmbh Molekulare Diagnostika Procede et necessaire d'essai pour la detection d'analytes dans un echantillon
US6531322B1 (en) 1998-08-13 2003-03-11 Lifescan, Inc. Visual blood glucose test strip
WO2003036293A1 (fr) 2001-10-26 2003-05-01 Chromeon Gmbh Procede de mesure optique simultanee d'un ph et d'une teneur en oxygene dissous
DE102007053664A1 (de) 2007-11-08 2009-05-14 Friedrich-Schiller-Universität Jena Optische Sensoren zur Detektion von Ionen, Gasen und Biomolekülen sowie Verfahren zu deren Herstellung und Verwendung

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019350A (en) * 1986-02-13 1991-05-28 Pfizer Hospital Products, Inc. Fluorescent polymers
US8313710B2 (en) * 2007-05-15 2012-11-20 Polestar Technologies, Inc. Multilayered optical sensing patch and retaining plug therefor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1603454A1 (de) 1965-11-24 1970-10-22 Mattel Inc Fasermaterial
US5853669A (en) 1991-09-30 1998-12-29 Merck Patent Gesellschaft Mit Beschrankter Haftung Sensor membrane for indicating the PH of a sample, the fabrication and use thereof
US6531322B1 (en) 1998-08-13 2003-03-11 Lifescan, Inc. Visual blood glucose test strip
WO2002035228A2 (fr) * 2000-10-27 2002-05-02 Attomol Gmbh Molekulare Diagnostika Procede et necessaire d'essai pour la detection d'analytes dans un echantillon
WO2003036293A1 (fr) 2001-10-26 2003-05-01 Chromeon Gmbh Procede de mesure optique simultanee d'un ph et d'une teneur en oxygene dissous
DE102007053664A1 (de) 2007-11-08 2009-05-14 Friedrich-Schiller-Universität Jena Optische Sensoren zur Detektion von Ionen, Gasen und Biomolekülen sowie Verfahren zu deren Herstellung und Verwendung

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANDREW BURNS ET AL: "Core/Shell Fluorescent Silica Nanoparticles for Chemical Sensing: Towards Single-Particle Laboratories", SMALL, vol. 2, no. 6, 1 June 2006 (2006-06-01), pages 723 - 726, XP055133830, ISSN: 1613-6810, DOI: 10.1002/smll.200600017 *
FENG WANG ET AL: "TOPICAL REVIEW; Luminescent nanomaterials for biological labelling", NANOTECHNOLOGY, IOP, BRISTOL, GB, vol. 17, no. 1, 14 January 2006 (2006-01-14), pages R1 - R13, XP020104209, ISSN: 0957-4484, DOI: 10.1088/0957-4484/17/1/R01 *
MCNAMARA K P ET AL: "SYNTHESIS, CHARACTERIZATION AND APPLICATION OF FLUORESCENCE SENSING LIPOBEADS FOR INTRACELLULAR PH MEASUREMENTS", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 73, no. 14, 15 July 2001 (2001-07-15), pages 3240 - 3246, XP001100438, ISSN: 0003-2700, DOI: 10.1021/AC0102314 *
STEPHANIE HORNIG ET AL: "Biocompatible fluorescent nanoparticles for pH-sensoring", SOFT MATTER, vol. 4, no. 6, 1 January 2008 (2008-01-01), pages 1169, XP055145340, ISSN: 1744-683X, DOI: 10.1039/b800276b *
YUKIKO TAKAHASHI ET AL: "Test Strips for Heavy-Metal Ions Fabricated from Nanosized Dye Compounds", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 45, no. 6, 30 January 2006 (2006-01-30), pages 913 - 916, XP008109097, ISSN: 1433-7851, DOI: 10.1002/ANIE.200503015 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019051205A1 (fr) * 2017-09-07 2019-03-14 Portland State University Capteurs à hydrogel permettant la détection d'ions métalliques
US11313843B2 (en) 2017-09-07 2022-04-26 Portland State University Hydrogel sensors for detection of metal ions
CN114460000A (zh) * 2020-11-10 2022-05-10 恩德莱斯和豪瑟尔分析仪表两合公司 用于确定溶液的pH值的指示剂

Also Published As

Publication number Publication date
AT514611B1 (de) 2016-08-15
AT514611A1 (de) 2015-02-15

Similar Documents

Publication Publication Date Title
DE69913103T2 (de) Optischer sensor und funktionsmethode
DE2851138C2 (fr)
DE2752352C3 (de) Prüfmittel zum Nachweis und zur Bestimmung einer Komponente in einer Probe
EP0175990B1 (fr) Membrane pour porter des réactifs, méthode pour sa préparation et son utilisation comme moyen analytique et dans des méthodes analytiques
EP2872876B1 (fr) Capteur opto-chimique et son utilisation
DE19629656A1 (de) Diagnostischer Testträger mit mehrschichtigem Testfeld und Verfahren zur Bestimmung von Analyt mit dessen Hilfe
EP3030886B1 (fr) Capteur optique et arrangement pour la détection quantitative d'un analyte
DE3630999A1 (de) Mehrschichtiger testtraeger
DE2518721A1 (de) Vorrichtung zur analyse von loesungen und koerperfluessigkeiten
DE1944246A1 (de) Verfahren und Vorrichtung zur quantitativen Bestimmung von chemischen Blutsubstanzen in Blutderivaten
EP0995994B1 (fr) Couches fonctionnelles à haute précision, méthode pour leur préparation et bandes d'essai contenants ces couches fonctionelles
EP0995992B1 (fr) Couches de diffusion, agents mouillants pour leurs préparation et leurs utilisation dans les bandes d'essai
DE102014107837A1 (de) Optischer Sensor zum quantitativen Nachweis eines Analyten in einer Probe und Verfahren zur Herstellung des Sensors
EP0309883A2 (fr) Bande d'analyse pour la détermination d'un composant dans un fluide corporel
DE3042857A1 (de) Mehrschichtiges chemisches analysenmaterial zur analyse waessriger fluessigkeiten
AT514611B1 (de) Sensormembran zur reversiblen Detektion von Analyten
DE3823151A1 (de) Verfahren zur bestimmung der ionenstaerke oder des spezifischen gewichts von waessrigen fluessigkeiten
DE4205876A1 (de) Testtraeger zur bestimmung eines analyten aus vollblut
DE2944980C2 (de) Zusammensetzung, Vorrichtung und Verfahren zum Bestimmen der Ionenstärke oder des spezifischen Gewichtes einer Flüssigkeitsprobe
DE3213183C2 (fr)
EP3578991B1 (fr) Essai de détermination de la concentration en phosphate
DE2948904A1 (de) Indikatorraum mit einem reaktionsraum
EP2636751A2 (fr) Procédé de détermination d'un liquide corporel
EP0358991B2 (fr) Dispositif de test pour la détermination optique de cations, d'anions ou de substances ionogènes électriquement neutres et procédé de test utilisant ce dispositif
DE112012001473B4 (de) Verfahren zum Messen einer Probenlösungs-Konzentration und Vorrichtung zum Messen einer Probenlösungs-Konzentration

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14757824

Country of ref document: EP

Kind code of ref document: A1

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14757824

Country of ref document: EP

Kind code of ref document: A1