WO2014164442A1 - Phytase - Google Patents

Phytase Download PDF

Info

Publication number
WO2014164442A1
WO2014164442A1 PCT/US2014/022432 US2014022432W WO2014164442A1 WO 2014164442 A1 WO2014164442 A1 WO 2014164442A1 US 2014022432 W US2014022432 W US 2014022432W WO 2014164442 A1 WO2014164442 A1 WO 2014164442A1
Authority
WO
WIPO (PCT)
Prior art keywords
seq
phytase
polypeptide
sequence
nucleic acid
Prior art date
Application number
PCT/US2014/022432
Other languages
English (en)
Inventor
Xuqiu Tan
Arne I. SOLBAK
Original Assignee
Verenium Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verenium Corporation filed Critical Verenium Corporation
Priority to PL14780237T priority Critical patent/PL2970925T3/pl
Priority to KR1020157028716A priority patent/KR102307173B1/ko
Priority to CN201480014287.1A priority patent/CN105229146B/zh
Priority to US14/771,143 priority patent/US9879238B2/en
Priority to EP14780237.5A priority patent/EP2970925B1/fr
Priority to BR112015021997A priority patent/BR112015021997A2/pt
Priority to ES14780237T priority patent/ES2806748T3/es
Priority to CA2910318A priority patent/CA2910318C/fr
Publication of WO2014164442A1 publication Critical patent/WO2014164442A1/fr
Priority to US15/879,379 priority patent/US10550371B2/en
Priority to US16/714,393 priority patent/US10982199B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)

Definitions

  • An embodiment of the invention is to provide a gene encoding a phytase which is produced at high levels in a gram-negative bacteria expression system. Another embodiment is to provide a gene encoding a phytase which is produced at high levels via intracellular expression. Another embodiment, is to produce a phytase in a gram-negative bacteria expression system, via intracellular expression, wherein the host organism is Pseudomonas. Another embodiment, is to produce a phytase in Pseudomonas fluorescens.
  • the expression system could be any Pseudomonas fluorescens expression system known in the art, for example, the Pseudomonas fluorescens expression system that is commercially available from Dow Global Technologies Inc., strain DC454 (US Patent PUB. APP. NO. 20050130160 and US Patent PUB. APP. NO. 20050186666).
  • a nucleic acid sequence encoding the phytase enzyme or polypeptide is inserted either in the pMYC vector (Dow Global Technologies Inc., US Patent PUB. APP. NO. 20050130160) or in the pDOW1169 vector (Dow Global Technologies Inc., US Patent PUB. APP. NO. 20080058262) and then introduced into the Pseudomonas fluorescens host by electroporation.
  • pMYC vector Wide Global Technologies Inc., US Patent PUB. APP. NO. 20050130160
  • pDOW1169 vector Wide Global Technologies Inc., US Patent P
  • the DNA encoding the phytase may be introduced, either on a plasmid or stably transformed into the genome of, for example, any number of gram negative bacterial systems such as E. coli, Pseudomonas species such as fluorescens, Pseudomonas putida, Pseudomonas aeruginosa, Ralstonia species, or Caulobacter species.
  • E. coli E. coli
  • Pseudomonas species such as fluorescens, Pseudomonas putida, Pseudomonas aeruginosa, Ralstonia species, or Caulobacter species.
  • a "codon” is a three polynucleotide sequence that specifies the identity of an amino acid to be added to a protein.
  • the mutations to be made in a protein of interest are determined by various factors including analysis of the two dimensional and three dimensional structure of the 5 prime end of the predicted mR A structure of an open reading frame (ORF) or gene of interest, as well as the preferred codons of the host, to select mutations which may enhance the expression of the protein of interest.
  • the mutations are not selected solely on the basis of the preferred codons of the host, that is, codon optimization, nor a codon optimization program.
  • a recombinant microorganism or cell culture is described as hosting an "expression vector" this includes both extra-chromosomal circular and linear DNA and DNA that has been incorporated into the host chromosome(s).
  • the vector may either be stably replicated by the cells during mitosis as an autonomous structure, or is incorporated within the host's genome.
  • the invention provides amplification primer sequence pairs for amplifying nucleic acids encoding polypeptides with a phytase activity, or subsequences thereof.
  • amplification primer sequence pairs for any part of or the full length of these sequences.
  • Amplification methods are also well known in the art, and include, e.g., polymerase chain reaction, PCR; transcription amplification; self-sustained sequence replication; Q Beta replicase amplification; and other RNA polymerase mediated techniques.
  • Variant includes derivatives or analogs of these polypeptides.
  • the variants may differ in amino acid sequence from the polypeptides of the invention, and sequences substantially identical thereto, by one or more substitutions, additions, deletions, fusions, and truncations, which may be present in any combination.
  • the supernatant was DNAse treated and buffer exchanged with 100 mM Tris pH 8.0.
  • the samples were then loaded onto an ion exchange column (FPLC) and fractions were collected. Eluted fractions were tested for activity and run on an SDS-PAGE; pure fractions were pooled.
  • Protein concentration was determined via spectrophotometry (absorbance at 280nm). The specific protein absorption coefficient (280nm) was determined using the peptide based software calculator, VECTOR NTI (Life Technologies, Carlsbad, CA).

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Epidemiology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)

Abstract

L'invention concerne des polypeptides ayant une activité phytase et des séquences polynucléotidiques codant pour les phytases. Le gène exprime la phytase à un taux d'au moins 7 g/L à 40 g/L. La phytase a une activité spécifique supérieure, conserve une activité à faible pH, et conserve une activité à température élevée. La phytase peut être utilisée dans une variété de compositions, comprenant un aliment, un aliment pour animaux, des produits pharmaceutiques, et de nettoyage.
PCT/US2014/022432 2013-03-12 2014-03-10 Phytase WO2014164442A1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
PL14780237T PL2970925T3 (pl) 2013-03-12 2014-03-10 Fitaza
KR1020157028716A KR102307173B1 (ko) 2013-03-12 2014-03-10 피타아제
CN201480014287.1A CN105229146B (zh) 2013-03-12 2014-03-10 肌醇六磷酸酶
US14/771,143 US9879238B2 (en) 2013-03-12 2014-03-10 Phytase
EP14780237.5A EP2970925B1 (fr) 2013-03-12 2014-03-10 Phytase
BR112015021997A BR112015021997A2 (pt) 2013-03-12 2014-03-10 Fitase
ES14780237T ES2806748T3 (es) 2013-03-12 2014-03-10 Fitasa
CA2910318A CA2910318C (fr) 2013-03-12 2014-03-10 Phytase
US15/879,379 US10550371B2 (en) 2013-03-12 2018-01-24 Phytase
US16/714,393 US10982199B2 (en) 2013-03-12 2019-12-13 Phytase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361777139P 2013-03-12 2013-03-12
US61/777,139 2013-03-12
GB1308828.1 2013-05-16
GB201308828A GB201308828D0 (en) 2013-03-12 2013-05-16 Phytase

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/771,143 A-371-Of-International US9879238B2 (en) 2013-03-12 2014-03-10 Phytase
US15/879,379 Division US10550371B2 (en) 2013-03-12 2018-01-24 Phytase

Publications (1)

Publication Number Publication Date
WO2014164442A1 true WO2014164442A1 (fr) 2014-10-09

Family

ID=48746850

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/022432 WO2014164442A1 (fr) 2013-03-12 2014-03-10 Phytase

Country Status (12)

Country Link
US (3) US9879238B2 (fr)
EP (1) EP2970925B1 (fr)
KR (1) KR102307173B1 (fr)
CN (2) CN105229146B (fr)
AR (1) AR095172A1 (fr)
BR (1) BR112015021997A2 (fr)
CA (1) CA2910318C (fr)
ES (1) ES2806748T3 (fr)
GB (1) GB201308828D0 (fr)
PL (1) PL2970925T3 (fr)
TW (2) TWI686479B (fr)
WO (1) WO2014164442A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103757019A (zh) * 2013-12-23 2014-04-30 青岛蔚蓝生物集团有限公司 一种启动子及表达外源蛋白的重组表达系统
WO2015197871A1 (fr) 2014-06-27 2015-12-30 Dsm Ip Assets B.V. Procede pour ameliorer la valeur nutritive d'aliments pour animaux
EP3027762A4 (fr) * 2013-07-25 2017-06-28 BASF Enzymes LLC Phytase
CN107287176A (zh) * 2016-04-12 2017-10-24 武汉新华扬生物股份有限公司 一种耐高温中性植酸酶Physh-A及其基因和应用
US9879238B2 (en) 2013-03-12 2018-01-30 Basf Enzymes Llc Phytase
US10518233B2 (en) 2013-03-14 2019-12-31 Basf Enzymes Llc Phytase formulation
CN112679581A (zh) * 2021-01-27 2021-04-20 华南农业大学 一种新型罗非鱼鳞促骨形成肽及其应用
EP3970506A1 (fr) 2015-07-02 2022-03-23 Novozymes A/S Compositions alimentaires pour animaux comprenant du lysozyme gh25 et de la phytase ec 3.1.3.26 et leurs utilisations

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3120360A1 (fr) 2018-11-20 2020-05-28 Dupont Nutrition Biosciences Aps Polypeptides robustes modifies a clade de phytase a tm elevee et fragments de ceux-ci
BR112022017034A2 (pt) 2020-02-28 2022-12-13 Dupont Nutrition Biosci Aps Composições de ração
CN113699134B (zh) * 2020-05-22 2024-04-05 潍坊康地恩生物科技有限公司 植酸酶突变体
CN112851758B (zh) * 2021-01-27 2022-03-22 华南农业大学 一种罗非鱼鳞促骨形成肽及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007813A2 (fr) * 2003-07-03 2005-01-27 Kemin Industries, Inc. Nouvelle phytase et gene correspondant
WO2010135588A2 (fr) * 2009-05-21 2010-11-25 Verenium Corporation Phytases, acides nucléiques codant pour elles et procédés de fabrication et d'utilisation associés

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1505388A (en) 1975-11-27 1978-03-30 Bp Chem Int Ltd Acid salt solutions
DE3587814T2 (de) 1985-03-30 1994-11-10 Marc Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
CA2098824A1 (fr) 1990-12-20 1992-06-21 William D. Huse Optimisation de proteines fixatrices
EP0626010A1 (fr) 1992-02-13 1994-11-30 Gist-Brocades N.V. Formulations liquides, aqueuses et stabilisees de phytase
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ATE332378T1 (de) * 1994-04-25 2006-07-15 Dsm Ip Assets Bv Polypeptide mit phytase wirkung
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5707843A (en) 1996-09-03 1998-01-13 Monte; Woodrow C. Lactose enzyme compositions and method
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US7432097B2 (en) 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US5876997A (en) 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6855365B2 (en) 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
ES2264181T3 (es) * 1997-10-01 2006-12-16 Dsm Ip Assets B.V. Procedimiento para la produccion de proteinas.
CN1197963C (zh) 1998-10-02 2005-04-20 诺维信公司 固态肌醇六磷酸酶组合物
PL343506A1 (en) 1999-02-10 2001-08-27 Dsm Nv Granulates containing feed-enzymes
JP2003514922A (ja) 1999-10-15 2003-04-22 ジェネンコア インターナショナル インコーポレーテッド 蛋白質含有顆粒および顆粒配合物
US6309872B1 (en) 2000-11-01 2001-10-30 Novozymes Biotech, Inc Polypeptides having glucoamylase activity and nucleic acids encoding same
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
TWI262083B (en) 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
MXPA04007886A (es) 2002-02-13 2004-10-15 Dow Global Technologies Inc Sobre-expresion de genes de extremozimo en pseudomonadas y bacterias estrechamente relacionadas.
CA2545610C (fr) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Bacterie pseudomonas fluorescens auxotrophique pour expression de proteine recombinante
KR100581318B1 (ko) 2003-12-15 2006-05-17 주식회사 중앙바이오텍 페니실리움 옥살리쿰 유래의 신규 파이테즈 유전자 및파이테즈를 생산하는 형질전환 피치아 파스토리스 균주
BRPI0507188A (pt) 2004-01-30 2007-06-26 Basf Ag formulação de enzima sólida ou lìquida estabilizada, processo para a preparação de gránulo (s) contendo enzimas, gránulo (s) contendo enzimas, processos para a preparação de uma ração animal, ou pré-mistura ou precursor para uma ração animal e para a preparação de uma composição, ou uma pré-mistura ou um precursor adequado para nutrição humana, uso de formulação sólida e/ou lìquida estabilizada, e, processo para promover o crescimento de um animal e/ou melhorar a taxa de conversão de alimento
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
DE102005043327A1 (de) 2005-09-12 2007-03-15 Basf Ag Verfahren zur Herstellung von festen Enzymgranulaten für Futtermittel
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
BRPI0711878A2 (pt) 2006-05-30 2012-01-10 Dow Global Technologies Inc método de otimização do códon
WO2008027099A2 (fr) 2006-05-30 2008-03-06 Dow Global Technologies Inc. Optimisation de rpa
EP2617814B1 (fr) 2006-09-21 2015-11-18 BASF Enzymes LLC Phytases, acides nucléiques encodant celles-ci et méthodes pour leur fabrication et leur utilisation
EP3031919B1 (fr) 2007-07-31 2018-09-19 BASF Enzymes LLC Assemblage combinatoire multi-sites sur mesure
CN101883500A (zh) * 2007-12-03 2010-11-10 先正达参股股份有限公司 工程化改造酶易感性蛋白质
CN101418276B (zh) 2008-12-08 2010-12-22 江南大学 一种宿主细胞及其用于重组蛋白高效分泌表达的方法
US20120107876A1 (en) 2009-05-01 2012-05-03 Lupin Limited Novel fusion tag offering solubility to insoluble recombinant protein
US8334124B1 (en) 2009-09-23 2012-12-18 The United States Of America As Represented By The Secretary Of Agriculture Modified Aspergillus niger phytase
US20130195829A1 (en) 2010-03-26 2013-08-01 Novozymes A/S Themostable Phytase Variants
WO2012025462A1 (fr) 2010-08-26 2012-03-01 Dsm Ip Assets B.V. Phytase dans des boissons gazeuses prêtes à boire
US8361956B2 (en) 2010-11-18 2013-01-29 Mohammad Ali Malboobi Recombinant APases nucleic acid sequences
CN102586294B (zh) 2011-01-12 2013-07-03 上海市农业科学院 一种适于毕赤酵母表达的高比活植酸酶基因及其制备方法和表达
WO2011141613A2 (fr) 2011-08-08 2011-11-17 Fertinagro Nutrientes, S.L. Nouvelle phytase, son procédé d'obtention et son utilisation
CN102943083B (zh) 2012-11-27 2013-12-25 青岛根源生物技术集团有限公司 一种定点突变的耐高温植酸酶基因tp及其表达载体和应用
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
AR095173A1 (es) 2013-07-25 2015-09-30 Basf Enzymes Llc Fitasa

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007813A2 (fr) * 2003-07-03 2005-01-27 Kemin Industries, Inc. Nouvelle phytase et gene correspondant
WO2010135588A2 (fr) * 2009-05-21 2010-11-25 Verenium Corporation Phytases, acides nucléiques codant pour elles et procédés de fabrication et d'utilisation associés

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2970925A4 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9879238B2 (en) 2013-03-12 2018-01-30 Basf Enzymes Llc Phytase
US10518233B2 (en) 2013-03-14 2019-12-31 Basf Enzymes Llc Phytase formulation
EP3027762A4 (fr) * 2013-07-25 2017-06-28 BASF Enzymes LLC Phytase
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
CN103757019A (zh) * 2013-12-23 2014-04-30 青岛蔚蓝生物集团有限公司 一种启动子及表达外源蛋白的重组表达系统
CN103757019B (zh) * 2013-12-23 2015-12-30 青岛蔚蓝生物集团有限公司 一种启动子及表达外源蛋白的重组表达系统
WO2015197871A1 (fr) 2014-06-27 2015-12-30 Dsm Ip Assets B.V. Procede pour ameliorer la valeur nutritive d'aliments pour animaux
US10980249B2 (en) 2014-06-27 2021-04-20 Dsm Ip Assets B.V. Method for improving the nutritional value of animal feed
EP3970506A1 (fr) 2015-07-02 2022-03-23 Novozymes A/S Compositions alimentaires pour animaux comprenant du lysozyme gh25 et de la phytase ec 3.1.3.26 et leurs utilisations
CN107287176A (zh) * 2016-04-12 2017-10-24 武汉新华扬生物股份有限公司 一种耐高温中性植酸酶Physh-A及其基因和应用
CN107287176B (zh) * 2016-04-12 2020-09-25 武汉新华扬生物股份有限公司 一种耐高温中性植酸酶Physh-A及其基因和应用
CN112679581A (zh) * 2021-01-27 2021-04-20 华南农业大学 一种新型罗非鱼鳞促骨形成肽及其应用

Also Published As

Publication number Publication date
EP2970925A4 (fr) 2016-11-09
BR112015021997A2 (pt) 2017-08-29
CN110577943A (zh) 2019-12-17
PL2970925T3 (pl) 2020-09-07
US20180258410A1 (en) 2018-09-13
CA2910318C (fr) 2021-05-25
ES2806748T3 (es) 2021-02-18
CA2910318A1 (fr) 2014-10-09
US20200248154A1 (en) 2020-08-06
EP2970925A1 (fr) 2016-01-20
US10982199B2 (en) 2021-04-20
EP2970925B1 (fr) 2020-05-06
US20160083700A1 (en) 2016-03-24
TW201512400A (zh) 2015-04-01
KR102307173B1 (ko) 2021-10-01
CN105229146B (zh) 2019-10-01
TW201907007A (zh) 2019-02-16
CN105229146A (zh) 2016-01-06
TWI686479B (zh) 2020-03-01
TWI635178B (zh) 2018-09-11
KR20150131206A (ko) 2015-11-24
US10550371B2 (en) 2020-02-04
AR095172A1 (es) 2015-09-30
US9879238B2 (en) 2018-01-30
GB201308828D0 (en) 2013-07-03

Similar Documents

Publication Publication Date Title
US10982199B2 (en) Phytase
US20190100734A1 (en) Phytase
US9109211B2 (en) Polypeptides having phytase activity and polynucleotides encoding same
US6475762B1 (en) Phytase enzymes nucleic acids encoding phytase enzymes and vectors and host cells incorporating same
US11214776B2 (en) Phytase mutant
CN110527676A (zh) 多肽，分离的其多核苷酸，以及包含多肽的添加剂、其用途及方法
WO2018025997A1 (fr) Hémicellulase
Tan et al. Cloning, overexpression, and characterization of a metagenome-derived phytase with optimal activity at low pH
Ghasemian et al. Production of recombinant microbial thermostable lipases
Bae et al. Molecular cloning and characterization of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723
AU2013254902A1 (en) Polypeptides having phytase activity and polynucleotides encoding same

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201480014287.1

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14780237

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 14771143

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2014780237

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 20157028716

Country of ref document: KR

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2910318

Country of ref document: CA

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112015021997

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112015021997

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20150909