WO2014131310A1 - 营养强化富硒大米及其栽培方法 - Google Patents
营养强化富硒大米及其栽培方法 Download PDFInfo
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- WO2014131310A1 WO2014131310A1 PCT/CN2013/090597 CN2013090597W WO2014131310A1 WO 2014131310 A1 WO2014131310 A1 WO 2014131310A1 CN 2013090597 W CN2013090597 W CN 2013090597W WO 2014131310 A1 WO2014131310 A1 WO 2014131310A1
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- Prior art keywords
- selenium
- cultivation method
- spraying
- protein
- rice
- Prior art date
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- 239000011669 selenium Substances 0.000 title claims abstract description 95
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 95
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 93
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 37
- 235000009566 rice Nutrition 0.000 title claims abstract description 37
- 238000012364 cultivation method Methods 0.000 title claims abstract description 19
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 36
- 235000015097 nutrients Nutrition 0.000 claims abstract description 34
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000005507 spraying Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 235000013343 vitamin Nutrition 0.000 claims abstract description 14
- 239000011782 vitamin Substances 0.000 claims abstract description 14
- 229940088594 vitamin Drugs 0.000 claims abstract description 14
- 229930003231 vitamin Natural products 0.000 claims abstract description 14
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 13
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000004368 Modified starch Substances 0.000 claims abstract description 9
- 229920000881 Modified starch Polymers 0.000 claims abstract description 9
- 235000019426 modified starch Nutrition 0.000 claims abstract description 9
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 3
- 230000000149 penetrating effect Effects 0.000 claims abstract description 3
- 229940091258 selenium supplement Drugs 0.000 claims description 91
- 235000018102 proteins Nutrition 0.000 claims description 38
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 108090000623 proteins and genes Proteins 0.000 claims description 38
- 239000002994 raw material Substances 0.000 claims description 28
- 239000000654 additive Substances 0.000 claims description 20
- 150000003384 small molecules Chemical class 0.000 claims description 15
- 230000000996 additive effect Effects 0.000 claims description 13
- 239000011573 trace mineral Substances 0.000 claims description 11
- 235000013619 trace mineral Nutrition 0.000 claims description 11
- 239000007921 spray Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 7
- 235000010755 mineral Nutrition 0.000 claims description 7
- 239000011707 mineral Substances 0.000 claims description 7
- -1 penetrants Substances 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical group [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- 235000013339 cereals Nutrition 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 239000011701 zinc Substances 0.000 claims description 4
- 229910052725 zinc Inorganic materials 0.000 claims description 4
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- OEUVSBXAMBLPES-UHFFFAOYSA-L calcium stearoyl-2-lactylate Chemical compound [Ca+2].CCCCCCCCCCCCCCCCCC(=O)OC(C)C(=O)OC(C)C([O-])=O.CCCCCCCCCCCCCCCCCC(=O)OC(C)C(=O)OC(C)C([O-])=O OEUVSBXAMBLPES-UHFFFAOYSA-L 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 229910052750 molybdenum Inorganic materials 0.000 claims description 3
- 239000011733 molybdenum Substances 0.000 claims description 3
- 150000003342 selenium Chemical class 0.000 claims description 3
- DNISEZBAYYIQFB-PHDIDXHHSA-N (2r,3r)-2,3-diacetyloxybutanedioic acid Chemical compound CC(=O)O[C@@H](C(O)=O)[C@H](C(O)=O)OC(C)=O DNISEZBAYYIQFB-PHDIDXHHSA-N 0.000 claims description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 239000003085 diluting agent Substances 0.000 claims description 2
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 2
- 239000006247 magnetic powder Substances 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 239000011572 manganese Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 239000011435 rock Substances 0.000 claims description 2
- JPJALAQPGMAKDF-UHFFFAOYSA-N selenium dioxide Chemical compound O=[Se]=O JPJALAQPGMAKDF-UHFFFAOYSA-N 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 229960001471 sodium selenite Drugs 0.000 claims description 2
- 239000011781 sodium selenite Substances 0.000 claims description 2
- 235000015921 sodium selenite Nutrition 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 229910052613 tourmaline Inorganic materials 0.000 claims description 2
- 229940070527 tourmaline Drugs 0.000 claims description 2
- 239000011032 tourmaline Substances 0.000 claims description 2
- 108010064851 Plant Proteins Proteins 0.000 claims 1
- 235000021120 animal protein Nutrition 0.000 claims 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 claims 1
- 235000010957 calcium stearoyl-2-lactylate Nutrition 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 150000002148 esters Chemical class 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 235000021118 plant-derived protein Nutrition 0.000 claims 1
- 229940080352 sodium stearoyl lactylate Drugs 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 22
- 239000000047 product Substances 0.000 description 12
- 235000013305 food Nutrition 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 7
- 230000035764 nutrition Effects 0.000 description 6
- 108010009736 Protein Hydrolysates Proteins 0.000 description 4
- 239000003531 protein hydrolysate Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000009469 supplementation Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 206010039921 Selenium deficiency Diseases 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
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- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 239000003223 protective agent Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000238876 Acari Species 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000000729 antidote Substances 0.000 description 1
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- 201000011510 cancer Diseases 0.000 description 1
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- 208000016097 disease of metabolism Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 238000010336 energy treatment Methods 0.000 description 1
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- 235000021321 essential mineral Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000014106 fortified food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000001935 peptisation Methods 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 208000017443 reproductive system disease Diseases 0.000 description 1
- 238000009287 sand filtration Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 235000010956 sodium stearoyl-2-lactylate Nutrition 0.000 description 1
- KNYAZNABVSEZDS-UHFFFAOYSA-M sodium;2-octadecanoyloxypropanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC(=O)OC(C)C([O-])=O KNYAZNABVSEZDS-UHFFFAOYSA-M 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/10—Fertilisers containing plant vitamins or hormones
Definitions
- Nutrition-enhanced selenium-enriched rice and cultivation method thereof are provided.
- the invention relates to a nutrient-enhanced selenium-enriched rice, in particular to a nutrient-enhanced selenium-enriched rice cultivated by a high-energy complex peptided protein selenium nutrient solution containing peptidated protein selenium, an energy promoter and other trace elements, and the rice Cultivation method.
- Selenium is hailed as "the fire of life” by the medical and nutrition circles at home and abroad, and enjoys the reputation of "longevity element", “king of anti-cancer", “heart patron” and “natural antidote”.
- Selenium is one of the essential trace elements in the human body. It is the active center of glutathione peroxidase (GSH-Px), which has the function of scavenging free radicals and anti-aging. In addition, selenium can improve human immunity and antagonize heavy metals. effect.
- Selenium is one-tenth of a million in human tissue, but it determines the existence of life. The great effect on human health is irreplaceable by other substances. Selenium deficiency directly leads to a decline in human immunity. Clinical medicine proves that more than 40 diseases that threaten human health and life are related to selenium deficiency in humans, such as cancer, cardiovascular disease, liver disease, cataract, endocrine metabolic disease, pancreatic disease. , diabetes, senile constipation, reproductive system diseases, etc.
- the Chinese Nutrition Society recommends a daily intake of 50 to 250 micrograms. However, the daily selenium intake of Chinese adults is only 26 to 50 micrograms. According to geologists, 72% of China's regions are selenium-deficient, and the content of selenium in natural foods such as grain is low; large and medium-sized cities such as North China, Northeast China, and Northwest China are all selenium-deficient areas, and vast areas of China's 22 provinces and cities. About 700 million people live in low-selenium areas.
- selenium there are three ways to supplement selenium: clinical selenium salt, difficult to master dose, and toxic side effects, difficult to promote application; taking selenium health products, such as selenium, gold partner, selenium capsule, etc., high prices, the majority of residents and The working class is economically unbearable; the selenium supplementation of food, that is, the supplementation of selenium through the fruits and foods that people often eat, is an economic, safe, efficient and convenient way to supplement selenium.
- the present invention provides a nutrient-enriched selenium-enriched rice which meets the national standard for selenium limit (GB13105-91), a grain oil material and a product thereof, 100-300 micrograms/kg, which is a good selenium-enriched health food.
- the present invention also provides a method for cultivating the rice.
- the cultivation method for the nutrient-enhanced selenium-enriched rice is characterized in that: the high-energy composite peptide-formed protein selenium nutrient solution and the small-molecule active water are diluted by 6000-8000 times, and sprayed three times by means of foliar spraying, each time required per acre 30 ⁇ 50 kg of diluted nutrient solution, the high-energy complex peptided protein selenium nutrient solution from selenium-containing raw material, protein hydrolysate, natural ore raw material, acetic acid, EDTA, penetrant, mineral additive, vitamin additive, organic Silicon, soluble modified starch, emulsifier, small molecule active water composition, the weight percentage of raw materials are: (1) 1-3% of selenium-containing raw materials, (2) 0.5% of natural ore raw materials.
- the spraying period is: the first spraying, spraying the dilution on the seedling page in the rice seedling stage; the second spraying, spraying on the rice leaf surface during the heading and flowering period; the third spraying Apply, sprayed on the rice leaf surface during the filling period.
- the second spraying is selected when the light is weak or in the afternoon or evening.
- the spraying is mechanical or artificial spray.
- the selenium-containing raw material is one of selenium element (SE), selenium oxide, sodium selenite, selenium salt, and organic selenium. Or a variety.
- SE selenium element
- selenium oxide sodium selenite
- selenium salt organic selenium. Or a variety.
- the natural ore raw material is one or more of medical stone, nano-scale magnetic powder, tourmaline, volcanic rock, and volcanic ash.
- the emulsifier is a monoglycoside: octadecanoic acid glycerol ester (English name Glycerin monostearate abbreviation: GMS), sodium stearoyl lactate (ssl), calcium stearoyl lactate (csl), diacetyl Tartaric acid monoglyceride (datem), sucrose fatty ester (se) or distilled monoglyceride (dmg).
- the penetrant is methanol or ethylene glycol.
- the protein is an animal or vegetable protein, a microbial protein or a peptidate.
- the composition of the trace element additive is zinc: 1-3%. Magnesium: 1-2%. Manganese: 1-2.5%. Molybdenum: 0.1-0.15%. ; II: 2-3%. .
- the composition of the vitamin additive is VC: 0.1%.
- VB 1, VD a total of 0.1.-0.2%. .
- the rice obtained by the above method was cultivated.
- the cultivation method for the nutrient-enhanced selenium-enriched rice of the invention adopts a self-made high-energy composite peptide-formed protein selenium nutrient solution, and sprays the rice seedling page three times to obtain a nutrient-enhanced selenium-enriched rice conforming to the standard.
- a method for using a high-energy complex peptided protein selenium nutrient solution Foliar application: The high-energy complex peptide protein selenium nutrient solution mother liquor is diluted 100 times with active water to prepare a stock solution. Take the appropriate amount of the original solution and add 60-80 times of the active water to dilute the nutrient solution diluted 30 ⁇ 50 kg per acre. Spray on the rice leaf page mechanically or artificially until the diluent is used up.
- the first step of the high-energy complex peptided protein selenium nutrient solution used in the invention is to enzymatically decompose the protein into an amino protein peptide, and the protein peptide and the selenium raw material act under the protection of the silicone protective agent to synthesize the peptided protein selenium.
- the compound, through the energy promoter, that is, the natural energy ore raw material, further enhances the energy of the product, and the nutrition absorbed by the crop is more comprehensive by adding vitamins and mineral additives, and the penetrating agent and the small molecule active water make the active ingredients faster and better. Absorbed.
- the high-energy complex peptided protein selenium nutrient solution not only greatly enhances the selenium-enriching ability of the crop, but also greatly improves the nutritional value, pest resistance and anti-corruption ability of the crop, and can increase the yield per acre by about 5-10%.
- the formula also adds protein, vitamins, minerals, energy promoters, etc., which greatly increases the content of essential mineral elements such as zinc, magnesium, manganese, molybdenum, boron and vitamins B, C and D in the crop, so that selenium is enriched.
- the rice surface is fine and delicate, the taste is very good, and the anti-oxidation and anti-bacteria ability is improved, so that the selenium-enriched agricultural products are resistant to storage and transportation.
- the selenium and boron elements in the formula are converted into phytonutrients, which are resistant to pathogens and pests, and have significant effects even on malignant pests such as nematodes, mites and fruit tree rot diseases, and can partially replace pesticides, thereby reducing the amount of pesticides and pesticide residues. Make selenium-enriched agricultural products easier to meet green standards.
- the small molecule active water is a multi-layer filtration of ordinary drinking water through sand filtration, carbon filtration, membrane filtration, etc., and then through an electro-optical ion exchange device with nanotechnology, separating the harmful acidic substances in the water.
- a water with a weak alkaline, small molecular group characteristics is a water with a weak alkaline, small molecular group characteristics.
- the small molecule water contributes to the sufficient dissolution of the above components and the rapid absorption of the crops, improves the absorption rate of the nutrient solution, and makes the crop nutrition comprehensively balanced.
- the selenium-enriched nutrient solution of the invention not only provides selenium for crops, but also provides various trace elements and energy promoters required for normal growth of crops, can promote crop growth and disease resistance, resist harmful substances, and improve crop quality and yield. .
- a nutrient-enriched selenium-enriched rice rich in selenium and other trace elements is cultivated in Rucheng County, Shandong province, and the rice seedlings are processed and harvested to obtain a finished product.
- the selenium content of selenium-enriched rice was tested by Shandong Analytical Testing Center (GB5009.93-2010), and the selenium content of the sample was 189 ⁇ g/kg.
- GB 13105-91 National standard for the regulation of selenium in food
- the high-energy complex peptided protein selenium nutrient solution mentioned in the present invention the selenium-containing raw material, the protein hydrolysate, the natural ore raw material, acetic acid, EDTA, penetrant, mineral additive, vitamin additive, silicone, soluble modified starch , emulsifier, small molecule active water composition
- the weight percentage of raw materials are: (1) 1-3% of selenium-containing raw materials, (2) 0.5% of natural ore raw materials. (3) 2-3% protein material, (4) 1 to 5% acetic acid.
- ( 6 ) 1% penetrant. (7) Trace element additives: 6-10%.
- Vitamin components 0.2-0.3%.
- Protein enzymatic hydrolysis enzymatic hydrolysis and peptization of the protein material to obtain an amino acid peptide which is a purified protein hydrolysate; the protein is enzymatically hydrolyzed by a neutral protease, the substrate concentration is 3%, and the neutral protein The enzyme concentration is 4.5%, the pH of the phosphate buffer solution is 7.0, the temperature is 50 ° C, and the enzyme is hydrolyzed for 1 to 2 hours. The undigested protein is separated by centrifugation to obtain the purified protein hydrolysate, that is, the amino protein peptide;
- step 2) Synthetic peptided protein selenium complex: In the substance formed in step 1), a silicone protective agent is added, and a selenium raw material is added, and uniformly stirred at 55 ° C for 1.5 hours;
- step 2) product, add EDTA, at 50 ⁇ 85 ° C, the reaction is 2.5h;
- Emulsification pretreatment The emulsifier is placed in a container and heated to melt into a liquid; 4 to 5 times the emulsifier weight part of the small molecule active water of about 70 ° C is added to the high-speed mixer, the mixer is started, and the hot water is intense Stirring; emulsifier that melts into a liquid, slowly added to the hot water being stirred and mixed, that is, a milky white hydrate paste can be formed, and then cooled to room temperature for use;
- Emulsification treatment Mix the products of step 4), step 5) and step 6), add appropriate amount of remaining small molecule active water, stir at room temperature for l h;
- a high-energy complex peptided protein selenium nutrient solution was prepared as described above. 1, 2 for nutrition-enhanced selenium-enriched rice cultivation
- the invention cultivates a nutrient-enhanced selenium-enriched rice rich in selenium and other trace elements in the Yucheng County of Shandong province through the following process steps. details as follows:
- the first spraying is carried out, and the configured high-energy complex peptided protein selenium nutrient solution is sprayed on the seedling leaf surface.
- Spraying amount 10 ⁇ 20kg/100 m 2 ;
- the rice seedlings are processed and harvested to obtain the finished product.
- the selenium content of selenium-enriched rice was tested by Shandong Analytical Testing Center (GB5009.93-2010), and the selenium content of the sample was 189 ⁇ g I kg.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
一种营养强化富硒大米的栽培方法,包括将由硒原料、蛋白质酶解产物、微量元素、天然矿石原料、醋酸、EDTA、维生素、有机硅、活性水、渗透剂、可溶性变性淀粉、乳化剂组成的营养液稀释后,在水稻秧苗期、抽穗开花期、灌浆结实期喷施在水稻叶面。由上述栽培方法获得的大米,其硒含量为189微克/公斤,每亩提高产量5-10%。
Description
营养强化富硒大米及其栽培方法 技术领域
本发明涉及一种营养强化富硒大米, 具体的说是利用含肽化蛋白硒、能量促 进剂及其他微量元素的高能复合肽化蛋白硒营养液培育的营养强化富硒大米,以 及该大米的栽培方法。
背景技术
硒被国内外医药界和营养学界尊称为"生命的火种", 享有"长寿元素"、 "抗癌 之王"、 "心脏守护神""天然解毒剂"等美誉。 硒是人体必需的微量元素之一, 它是 谷胱甘肽过氧化物酶 (GSH-Px)活性中心, 具有清除自由基、 抗衰老作用, 另外, 硒能够提高人体免疫力, 对重金属有拮抗作用。
硒在人体组织内含量为千万分之一,但它却决定了生命的存在, 对人类健康 的巨大作用是其他物质无法替代的。缺硒会直接导致人体免疫能力下降, 临床医 学证明, 威胁人类健康和生命的四十多种疾病都与人体缺硒有关, 如癌症、 心血 管病、 肝病、 白内障、 内分泌代谢病、 胰脏疾病、 糖尿病、 老年性便秘、 生殖系 统疾病等等。
中国营养学会推荐日摄入量为 50~250微克。 但我国成人的每日硒摄入量仅 有 26~50微克。 据地质学家考证: 中国 72%的地区属于缺硒地区, 粮食等天然 食物硒含量较低; 华北、 东北、 西北等大中城市都属于缺硒地区, 中国二十二个 省市的广大地区, 约七亿人生活在低缺硒地区。
WHO公布的资料表明, 全球有 40多个国家属于低硒或缺硒地区。 富硒农 副产品是人体补充硒元素有效途径, 因此, 富硒技术是科学家们研究开发的热点 领域。
低于推荐值。据专家考证,人需要终生补硒。无论是动物实验还是临床实践, 都说明了应该不断从饮食中得到足够量的硒,不能及时补充,就会降低祛病能力。
人应该像每天必须摄取淀粉、 蛋白质和维生素一样, 每天必须摄入足够量的硒。 因此, 补硒已经成为我们追寻健康的一种潮流, 也是势在必行的健康使命。
补硒的途径有三种: 临床补硒盐, 剂量难掌握, 且毒副作用大, 难以推广应 用; 服用含硒保健品, 如富硒康、 黄金搭档、 硒旺胶囊等, 价格高, 广大居民和 工薪阶层经济上难以承受; 食物补硒, 即通过人们经常食用的水果、粮食等来补 充硒, 是一种经济、 安全、 高效、 方便的补硒途径。
现在市场上己出现有富硒粮食,但多数采用无机硒和络合硒制剂, 其吸收率 低, 产能小。作为我国最主要的粮食作物大米, 也是我国大多数地区居民的常用 主食原料, 采用营养强化型富硒大米, 将对广大居民的健康提供有力的保障。 发明内容
针对上述领域中的需求, 本发明提供一种营养强化富硒大米, 符合国家规 定硒限量卫生标准( GB13105-91)粮食油料及其制品 100-300微克 /千克, 为良好 的富硒保健食品。
同时本发明还提供该大米的栽培方法。
营养强化富硒大米的栽培方法,其特征在于: 将高能复合肽化蛋白硒营养液 加小分子活性水稀释 6000-8000倍, 采用叶面喷施的方式, 喷洒 3次, 每次每亩 需要 30〜50公斤稀释好的营养液,所述高能复合肽化蛋白硒营养液由含硒原料, 蛋白质的酶解产物, 天然矿石原料, 醋酸, EDTA、 渗透剂, 矿物质添加剂, 维 生素添加剂, 有机硅, 可溶性变性淀粉, 乳化剂, 小分子活性水组成, 其原料重 量百分比为: (1 ) 含硒原料 1-3%, (2) 天然矿石原料 0.5%。, ( 3 ) 蛋白质原料 2-3% , (4) 醋酸 1~5%。, ( 5 ) EDTA 0.5~l%o, ( 6) 渗透剂 1%。, (7 ) 微量元素添 加剂 6-10%。, ( 8 ) 维生素添加剂 0.2-0.3%。. ( 9) 有机硅 1%。。 ( 10) 可溶性变性 淀粉 1%, ( 11 ) 乳化剂 1.5%— 5%, ( 12) 小分子活性水: 87-93%
所述喷施期间分别为: 第一次喷施, 在水稻秧苗期, 将稀释液喷施于秧苗 页面; 第二次喷施, 在抽穗开花期, 喷洒在水稻叶面上; 第三次喷施, 在灌浆 结实期, 喷洒在水稻叶面上。
所述第二次喷施时选择在光照较弱时或下午、 傍晚。
所述喷施为机械或人工雾状喷施。
所述含硒原料为硒元素 (SE)、 氧化硒、 亚硒酸钠、 硒盐、 有机硒中的一种
或多种。
所述天然矿石原料为麦饭石、 纳米级磁粉、 电气石、 火山岩、 火山灰中的一 种或多种。
所述的乳化剂为单苷酯: 单十八 (烷) 酸丙三醇酯 (英文名 Glycerin monostearate 简称: GMS ) , 硬脂酰乳酸钠 (ssl)、 硬脂酰乳酸钙 (csl)、 双乙酰 酒石酸单甘油酯 (datem)、 蔗糖脂肪酯 (se) 或蒸馏单甘酯 (dmg)。
所述渗透剂为甲醇或乙二醇。
所述的蛋白质为动植物蛋白、 微生物蛋白或肽化物。
所述的微量元素添加剂的组成为锌: 1-3%。; 镁: 1-2%。; 锰: 1-2.5%。; 钼: 0.1-0.15%。; II: 2-3%。。
所述维生素添加剂的组成为 VC: 0.1%。; VB 1、 VD总共为 0.1.-0.2%。。 采用上述方法栽培得到的大米。
本发明营养强化富硒大米的栽培方法, 采用自制的高能复合肽化蛋白硒营 养液, 分三次喷施稻苗页面, 得到符合标准的营养强化富硒大米。
高能复合肽化蛋白硒营养液的使用方法。 叶面喷施: 将高能复合肽化蛋白 硒营养液母液加活性水稀释 100 倍, 配制成原液。 取原液适量再加活性水稀释 60-80倍, 每亩需要 30〜50公斤稀释好的营养液。 以机械或人工喷雾于水稻叶 页, 直到稀释液用完为止。
本发明采用的高能复合肽化蛋白硒营养液第一步是通过酶解将蛋白质酶解 为氨基蛋白肽,此蛋白肽与硒原料在有机硅保护剂的保护下, 发生作用合成肽化 蛋白硒复合物, 通过能量促进剂即天然能量矿石原料, 使产品能量进一步提升, 通过添加维生素、矿物质添加剂使作物吸收的营养更全面, 渗透剂、 小分子活性 水使各有效成分能更快更好地吸收。该高能复合肽化蛋白硒营养液不仅使作物富 硒能力大大提高, 作物的营养价值、 抗病虫害能力、 抗腐败能力都大大提高, 每 亩可提高产量 5-10%左右。
其配方中同时添加了蛋白质、 维生素、 矿物质、 能量促进剂等, 大大提高了 作物中人体必需的矿物元素锌、 镁、 锰、 鉬、 硼及维生素 B、 C、 D的含量, 使 富硒大米表光细腻, 口感甚佳, 抗氧化、 抑病菌能力提高, 使富硒农产品耐储耐 运。
其配方中的硒及硼元素等转化为植物营养素, 可抵御病菌和害虫, 即使对线 虫、 韭蛆和果树腐烂病等恶性病虫害也有显著效果, 可部分代替农药, 使农药用 量和农药残留减少, 使富硒农产品更容易达到绿色标准。
所述的小分子活性水, 就是将普通饮用水经过砂滤、炭滤、膜滤等多层过滤 后, 再经过具有纳米技术的电生离子交换设备, 将水中对人体有害的酸性物质分 离出去, 而保留水中的矿物质离子, 是具有弱碱性、 小分子团特征的水。 小分子 水有助于上述组分的充分溶解及作物的快速吸收, 提高物质对营养液的吸收率, 使作物营养全面均衡。
利用本发明富硒营养液不仅为作物提供硒,还可提供农作物正常生长所需要 的多种微量元素及能量促进剂,可促进农作物的生长和抗病能力,抵御有害物质, 提高作物质量及产量。
采用上述栽培方法,在山东郯城县培育富含硒元素及其他微量元素的营养强 化富硒大米,稻苗成熟收割后经过加工即得成品。富硒大米的硒含量经山东省分 析测试中心检测 (GB5009.93-2010 ) , 送检样品硒含量为 189微克 /公斤。 完全 复合国家规定食品中硒限量卫生标准( GB 13105-91)粮食油料及其制品 100-300 微克 /千克。 具体实施方式
下面通过实施例对本发明作进一步说明。
本发明中提到的高能复合肽化蛋白硒营养液, 由含硒原料, 蛋白质的酶解产 物, 天然矿石原料, 醋酸, EDTA、 渗透剂, 矿物质添加剂, 维生素添加剂, 有 机硅, 可溶性变性淀粉, 乳化剂, 小分子活性水组成, 其原料重量百分比为: (1 ) 含硒原料 1-3%,(2)天然矿石原料 0.5%。,(3 )蛋白质原料 2-3%,(4)醋酸 1~5%。, ( 5 ) EDTA 0.5~1%0, ( 6 ) 渗透剂 1%。, ( 7 ) 微量元素添加剂: 6-10%。, (8 ) 维 生素组分: 0.2-0.3%。. ( 9 )有机硅 1%。。 ( 10 )可溶性变性淀粉 1%。(11 )乳化剂: 1.5 -5 ( 12) 小分子活性水: 87-93%
配制方法:
1 ) 蛋白质酶解: 对蛋白质原料进行酶解肽化处理, 得到纯化蛋白质酶解物即 氨基蛋白肽; 所述蛋白质酶解为采用中性蛋白酶酶解, 底物浓度 3%, 中性蛋白
酶浓度 4.5%, 磷酸盐缓冲溶液 PH值 7.0, 温度 50°C, 酶解 1~2小时, 离心分 离未酶解蛋白质, 获得纯化蛋白质酶解物, 即氨基蛋白肽;
2) 合成肽化蛋白硒复合物: 在步骤 1 ) 中所生成的物质中, 添加有机硅保护 剂, 添加硒原料, 在 55°C情况下, 均匀搅拌 1.5h;
3) 能量化处理: 将天然矿石原料、 微量元素添加剂、 醋酸、 可溶性变性淀粉 混合在一起, 在 42~70°C情况下, 均匀搅拌 lh;
4) 络合反应: 步骤 2)产物中,加上 EDTA, , 在 50~85°C情况下, 反应 2.5h;
5) 强化处理: 步骤 3)产物中, 添加渗透剂、 维生素添加剂, 在常温下搅拌 1 h;
6) 乳化预处理: 将乳化剂置于容器内, 加热熔化成液体; 将 4一 5倍于乳化 剂重量份的约 70°C的小分子活性水加入高速搅拌机, 启动搅拌机, 将热 水激烈搅动; 将熔化成液体的乳化剂, 徐徐加入正在被搅拌的热水中搅打 混合, 即可以生成乳白的水合物膏体, 后冷却到室温待用;
7) 乳化处理: 将步骤 4)、 步骤 5) 和步骤 6) 产物混合, 加上适量其余小分 子活性水, 室温搅拌 l h;
8) 检验合格, 灭菌后, 制作成合格的高能复合肽化蛋白硒营养液母液储存。
以下实施例仅用于阐述本发明, 而本发明的保护范围并非仅仅局限于以下 实施例。所述技术领域的普通技术人员依据以上本发明的公开内容, 均可实现本 发明的目的。
实施例 1
1、 1原料配料
( 1 )含硒原料 2KG, (2)天然矿石原料 0.05KG, (3)蛋白质原料 2KG, (4) 醋酸 0.3KG, (5) EDTA 0.1KG, ( 6)渗透剂 0.1KG, (7) 微量元素添加剂, 锌: 0.1KG; 镁: 0.1KG; 猛: 0.2KG; ·目: 0.01KG; II: 0.21KG, ( 8) 维生素添 加剂, VC: 0.01KG; VB1、 VD总共为 0.02KG, (9)有机硅 0.1KG, ( 10)可溶 性变性淀粉 1KG, ( 11 ) 乳化剂 3KG, ( 12) 小分子活性水约为 90.7KG, 配料总 共为 100KG。
按上述方法制备得到高能复合肽化蛋白硒营养液。
1、 2 进行营养强化富硒大米栽培
本发明通过以下的工艺步骤,在山东郯城县培育富含硒元素及其他微量元素 的营养强化富硒大米。 具体如下:
1、 先制备叶面喷施营养液: 利用发明内容中所提到的高能复合肽化蛋白硒营养 液,加小分子活性水稀释 100倍,配制成原液。取原液适量再加活性水稀释 60~80 倍, 稀释好的高能复合肽化蛋白硒营养液。
2、 在水稻秧苗期, 进行第一次喷洒, 将配置好的高能复合肽化蛋白硒营养液喷 施在秧苗叶面上。 喷施的用量 10〜20kg/100 m2;
3、在抽穗开花期进行第二次喷洒, 喷洒在水稻叶面上,每亩地喷洒营养液 30-50 kg; 喷洒的时间选择在光照较弱时或下午、 傍晚。
4、在灌浆结实期,也就是在第二次喷洒后间隔 5-7天喷洒第三次,每亩需要 30〜 50 kg, 15-20天后即可收割。
经过上述各步骤处理后,稻苗成熟收割后经过加工即得成品。富硒大米的硒 含量经山东省分析测试中心检测 (GB5009.93-2010), 送检样品硒含量为 189微 克 I公斤。 完全复合国家规定食品中硒限量卫生标准( GB13105-91)粮食油料及 其制品 100-300微克 /千克。
Claims
1、 营养强化富硒大米的栽培方法, 其特征在于: 将高能复合肽化蛋白硒营 养液加小分子活性水稀释 6000-8000倍, 采用叶面喷施的方式, 喷洒 3次, 每次 每亩需要 30〜50公斤稀释好的营养液, 所述高能复合肽化蛋白硒营养液由含硒 原料, 蛋白质的酶解产物, 天然矿石原料, 醋酸, EDTA、 渗透剂, 矿物质添加 剂, 维生素添加剂, 有机硅, 可溶性变性淀粉, 乳化剂, 小分子活性水组成, 其 原料重量百分比为: (1 )含硒原料 1-3%, (2) 天然矿石原料 0.5%。, ( 3 ) 蛋白质 原料 2-3%, (4)醋酸 1~5%。, ( 5 ) EDTA 0.5~1%。, ( 6)渗透剂 1%。, (7 )微量元 素添加剂 6-10%。, ( 8 ) 维生素添加剂 0.2-0.3%。. ( 9) 有机硅 1%。。 ( 10) 可溶性 变性淀粉 1%, ( 11 ) 乳化剂 1.5%— 5%, ( 12) 小分子活性水: 87-93%
2、 根据权利要求 1所述的栽培方法, 所述喷施期间分别为: 第一次喷施, 在水稻秧苗期, 将稀释液喷施于秧苗页面; 第二次喷施, 在抽穗开花期, 喷洒 在水稻叶面上; 第三次喷施, 在灌浆结实期, 喷洒在水稻叶面上。
3、 根据权利要求 2所述的栽培方法, 所述第二次喷施时选择在光照较弱时 或下午、 傍晚。
4、 根据权利要求 1所述的栽培方法, 所述喷施为机械或人工雾状喷施。
5、 根据权利要求 1所述的栽培方法, 所述含硒原料为硒元素、 氧化硒、 亚 硒酸钠、 硒盐、 有机硒中的一种或多种。
6、 根据权利要求 1所述的栽培方法, 所述天然矿石原料为麦饭石、 纳米级 磁粉、 电气石、 火山岩、 火山灰中的一种或多种。
7、 根据权利要求 1所述的栽培方法, 所述的乳化剂为单苷酯: 单十八烷酸 丙三醇酯, 硬脂酰乳酸钠、 硬脂酰乳酸钙、 双乙酰酒石酸单甘油酯、 蔗糖脂肪酯 或蒸馏单甘酯。
8、 根据权利要求 1所述的栽培方法, 所述渗透剂为甲醇或乙二醇。
9、 根据权利要求 1所述的栽培方法, 所述的蛋白质为动植物蛋白、 微生物 蛋白或肽化物。
10、 根据权利要求 1所述的栽培方法, 所述的微量元素添加剂的组成为锌: 1-3%。; 镁: 1-2%。; 锰: 1-2.5%。; 钼: 0.1-0.15%。; 硼: 2-3%。。
11、根据权利要求 1所述的栽培方法,所述维生素添加剂的组成为 vc: 0.1
VB1、 VD总共为 0.1.-0.2%。。
12、 采用权利要求 1-11任一方法栽培得到的大米。
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