WO2014106309A1 - 一种检测前列腺液游离血红素的方法及检测盒 - Google Patents
一种检测前列腺液游离血红素的方法及检测盒 Download PDFInfo
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- WO2014106309A1 WO2014106309A1 PCT/CN2013/000070 CN2013000070W WO2014106309A1 WO 2014106309 A1 WO2014106309 A1 WO 2014106309A1 CN 2013000070 W CN2013000070 W CN 2013000070W WO 2014106309 A1 WO2014106309 A1 WO 2014106309A1
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- prostatic fluid
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- sampling sleeve
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- the present invention relates to a detection method, and in particular to a method for detecting free hemoglobin in prostatic fluid, and to a special detection kit for detecting free hemoglobin in prostatic fluid.
- Free heme in prostatic fluid is often caused by the release of free hemoglobin from cytochromes, oxygen receptors and FixL transmembrane proteins of tissue tumor cells, precipitation or tumor tissue microcirculation exudation, and its free heme content.
- cytochromes, oxygen receptors and FixL transmembrane proteins of tissue tumor cells precipitation or tumor tissue microcirculation exudation, and its free heme content.
- FixL transmembrane proteins of tissue tumor cells precipitation or tumor tissue microcirculation exudation, and its free heme content.
- Very few, invisible to the naked eye generally required to use advanced instruments and analytical chemistry for testing, high cost, long cycle, so invent a simple, low-cost detection method to quickly determine whether the prostatic fluid contains free heme , become an urgent problem to be solved.
- the present invention provides a method for detecting free hemoglobin in prostatic fluid, and the method provided by the invention has high sensitivity.
- the invention is low in cost and can quickly detect free hemoglobin in the prostatic fluid; and the invention also provides a detection box with a special sampling sleeve for detecting free hemoglobin in the prostatic fluid.
- the method for detecting free hemoglobin in prostatic fluid comprises the following detection steps:
- the distal end of the finger is massaged inward and forward, about 4-5 times on each side, and then the hand is moved to the upper part of the gland and squeezed down the middle groove, so that the prostatic fluid can be discharged to the prostatic fluid sampling set.
- the prostatic fluid sampling set On the top, take the specimen for inspection; wherein, the amount of prostate fluid taken is 0.5-1 ml;
- the preparation method of the reagent A is as follows: Take 3,3', 5.5'-tetraf if3 ⁇ 4 benzidine 50mg dissolved ten PH is 7.4 citric acid buffer 100 ml of the solution was mixed; the reagent B was a 3% hydrogen peroxide solution.
- the present invention also provides a detection kit for detecting free hemoglobin in prostatic fluid.
- the invention relates to a test box for detecting free hemoglobin of prostate, comprising a box body and an outer package box outside the outer box; the outer surface of the outer package box is provided with a color swatch plate, and the box body is provided with a scorpion storage body which is recessed inwardly a sample cup storage area, a dropper storage area and a test cell, wherein the test cell stores a columnar gland sampling sleeve, the prostatic fluid sampling sleeve has a cylindrical shape closed at the bottom end, and the bottom end is further provided with a convex portion a small pouch.
- the prostate sampling sleeve is made of an absorbent nonwoven fabric.
- the prostatic fluid sampling sleeve has a diameter of 3.5 cm and a length of 5 cm, and the small capsule has a diameter of lcm and a depth of 0.5 cm.
- test pool is disposed above the test box, having a diameter of 1.8 cm and a depth of 1.2 cm.
- the dropper storage area is disposed on the two book sides of the test cell, the sample cup storage area is disposed on the left side of the test cell, and the scorpion placement area is disposed on the left side of the sample cup storage area.
- a dropper is arranged in the dropper storage area, and the dropper is provided with a scale.
- a sample cup is disposed in the sample cup placement area, and the sample cup is provided with a sealing cover, and the cup of the sample cup has a cylindrical shape.
- the dice storage area is provided with a dice, and the dice are plastic crucibles.
- the detection method provided by the invention is simple and practical, low in cost, and can quickly screen a sample containing no free hemoglobin in a prostatic fluid, a sample containing free hemoglobin, and a sample which may contain free hemoglobin,
- the preparation of the test can be carried out; the lowest free hemoglobin concentration detectable by the invention can reach 0.5 ng/L, and the sensitivity is high; and the present invention also provides a sample of glandular fluid sampling sleeve, which can be used for collecting the glandular fluid;
- the invention also provides a detection box, which can be used exclusively for holding the utensils and reagents used in the detection process.
- the kit has a simple structure, and the entire detection step can be completed with only one kit, saving space and reagents. At the same time, it also reduces the operator's labor and throws.
- 1 is a schematic structural view of an outer package.
- FIG. 2 is a schematic structural view of a casing.
- FIG. 3 is a schematic view showing the structure of a gland liquid sampling sleeve.
- the subject is taken to the chest position, and the outer box of the test box is opened.
- the prostate fluid sampling sleeve 8 is taken out from the box body 3, and is placed over the coronary sulcus at the glans head of the subject.
- the examiner supports the left shoulder or buttocks of the patient in the left hand, gloves or fingertips in the right hand, Vaseline or liquid paraffin on the fingertips, the index finger is gently massaged at the anus, then the fingers are slowly inserted into the anus, and the fingertip enters the rectum about 5 cm away from the anus.
- the prostate can be touched at the front wall, and the inner end of the finger is massaged inward and forward, about 45 times on each side, and then the hand is moved to the upper part of the gland and squeezed down the middle groove, so that the prostatic fluid can be
- the mountain urethra is discharged to the prostate fluid sampling sleeve 8, and the specimen is taken for inspection.
- the amount of prostatic fluid taken is 0.5-1 ml; the sample cup of the sample cup placement area 5 is prefilled with 3 ml of tris (hydroxymethylaminomethane-hydrochloric acid buffer) having a pH of 7.4, and the solution with prostate fluid is placed.
- the prostate sampling sleeve 8 is placed in the sample cup and mixed; two dropper tubes are taken out from the dropper storage area 6, and the two droppers are pre-loaded with reagent A and reagent B, respectively, and the sample liquid is sequentially added in the test tank 7 , reagent A 0.5ml, reagent B 0.5ml, mix, observe the color change within 2 minutes, compare with the colorimetric plate 2 of the test box packaging, and record the test result;
- the preparation method of the reagent A is as follows: 50 mg of 3',5,5'-tetramethylbenzidine was dissolved in 100 ml of a pH 4.4 citrate buffer; the reagent B was a 3% hydrogen peroxide solution.
- the detection result is determined as follows:
- the detection method of the permeate having a free hemoglobin content of 0.5 ng/L or more can be initially determined, and the detection method of the exudate having a free hemoglobin content of 20 ng/L or more can be used. It is determined that the exudate contains free hemoglobin, so the sensitivity of the detection method is high.
- a detection kit for detecting a free hemoglobin of a gland comprising a casing 3 and an outer casing 1 outside the casing, the outer surface of the outer casing 1 being provided with a color palette 2, the casing being arranged inwardly
- the trapped tweezers store Lx: 4, the sample cup storage 5, the dropper storage area 6 and the test cell 7, the test cell 7 stores the prostatic fluid sampling sleeve 8, and the prostatic fluid sampling sleeve 8 is closed at the bottom end.
- the bottom end is further provided with a small capsule 9 of ⁇ "
- the column of the gland liquid sampling sleeve 8 is adsorbed Description
- the non-woven fabric, the prostatic fluid sampling sleeve 8 has a diameter of 3.5 cm and a length of 5 cm, and the small capsule 9 has a diameter of 1 cm and a depth of 0.5 cm.
- the test cell 7 is disposed above the box body 3 and has a diameter of 1.8 cm. 1.2cm, the dropper storage area 6 is disposed on both sides of the test cell 7, the sample cup storage area 5 is disposed on the left side of the test cell 7, and the forceps placement area 4 is disposed on the left side of the sample cup storage area 5 A dropper is arranged in the dropper storage area 6, and the dropper is provided with a scale.
- the sample cup placement area 5 is provided with a sample cup.
- the sample cup is provided with a sealing cover, and the sample cup has a cylindrical shape.
- the raft storage area 4 is provided with a rafter, and the rafter is a plastic raft.
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Abstract
本发明提供了一种检测前列腺液游离血红素的的方法，包括获取前列腺液渗液；用PH为7.4的三羟甲基氨基甲烷-盐酸缓冲液处理渗液；后再与显色试剂反应再与比色板比对记录检测结果；本发明简单实用，成本低廉，可快速的筛查出宫颈渗液中不含血红素的样本、含血红素的样本和可能含有血红素的样本，为进一步筛查做准备。同时本发明还涉及一种检测前列腺液渗液的检测盒。
Description
说 明 一种检测前列腺液游离血红素的方法及检测盒 技术领域
[0001] 本发明涉及一种检测方法， 具体涉及一种检测前列腺液游离血红素的方法， 同时本 发明还涉及一种检测前列腺液游离血红素的专用检测盒。
背景技术
[0002] 前列腺液中的游离血红素往往是由组织肿瘤细胞的细胞色素、 氧感受器和 FixL跨膜 蛋白的游离血红素释放、 析出或肿瘤组织微循环渗出引起的， 其游离血红素的含量极少， 肉 眼不可得见， 一般需借助先进的仪器和分析化学方法进行检测， 费用高， 周期长， 所以发明 一种操作简单， 成本低廉的检测方法快速的确定前列腺液中是否含有游离血红素， 成为一个 亟需解决的问题。
发明内容
[0003] 为了解决现有技术中检测前列腺液中游离血红素的方法费用高、 周期长的问题， 本 发明提供了一种检测前列腺液游离血红素的方法， 本发明提供的方法其灵敏度高， 成本低 廉， 可快速的检测前列腺液中的游离血红素； 同时本发明还提供了一种检测前列腺液中游离 血红素的带有专用取样套的检测盒。
[0004] 本发明的技术方案如下：
本发明提供的检测前列腺液中游离血红素的方法， 包括以下检测歩骤：
1) 使受检者取膝胸位， 打开检测盒的外包装盒， 从盒体内取出前列腺液取样套， 套在受 检者阴茎龟头部位超过冠状沟， 检査者左手扶持患者左肩或臀部， 右手戴手套或指套， 指端 涂凡士林或液体石蜡； 食指先在肛门口轻轻按摩， 然后将手指徐徐插入肛门， 当指端进入距 肛门门约 5cm直肠前壁处即可触及前列腺， 以手指末节作向内、 向前徐徐按摩， 每侧约 4-5 次， 然后再将手移至腺体的上部顺正中沟向下挤压， 这样前列腺液即可 ώ尿道排出至前列腺 液取样套上， 留取标本送检； 其中， 所取前列腺液量为 0.5-1 ml;
2) 把带有前列腺液的前列腺液取样套放入样品杯内， 样品杯内预装有三羟甲基氨基甲烷- 盐酸缓冲液， 缓冲液的量为 3ml， 混匀， 得样品液； 优选的， 三羟甲基氨基甲垸 -盐酸缓冲液 的 PH值为 7.4 ；
3 ) 在测试池内顺序加入样品液 lml， 试剂 A 0.5ml， 试剂 B 0.5ml ; 混匀， 2分钟内观察颜 色变化， 与比色板比对 i ^检测结果；
所述试剂 A的配制方法如卜： 取 3，3'，5.5'-四 if ¾联苯胺 50mg溶十 PH为 7.4柠檬酸缓冲
液 100ml混匀而成； 所述试剂 B为 3%的过氧化氢溶液。
[0005】 本发明同时提供了一种检测前列腺液游离血红素的检测盒。
一种检测前列腺游离血红素的检测盒， 包括盒体和盒体外的外包装盒， 所述外包装盒的外表 面设有比色板， 所述盒体设有向内凹陷而成的镊子存放区、 样品杯存放区、 滴管存放区和测 试池， 所述测试池内存放有 列腺液取样套， 所述前列腺液取样套呈底端封闭的圆筒状， 所 述底端还设有一凸起的小囊。
[0006] 进一歩的， 所述前列腺取样套材质为吸附性无纺布。
[0007] 进一歩的， 所述前列腺液取样套的直径 3.5cm， 长度 5cm， 所述小囊直径 lcm， 深度 0.5cm。 说
[0008] 进一步的， 所述测试池设于测试盒中上方， 直径 1.8cm， 深度 1.2cm。
[0009] 进一步的， 所述滴管存放区设于测试池两书侧， 所述样品杯存放区设于测试池左侧， 所述镊子放置区设于样品杯存放区左侧。
[0010] 进一歩的， 所述滴管存放区内设有滴管， 滴管上带有刻度。
[0011] 进一歩的， 所述样品杯放置区内设有样品杯， 所述样品杯设有密封盖， 样品杯的杯体 呈圆筒状。
[0012] 进一歩的， 所述镊子存放区内设有镊子， 所述镊子为塑料镊。
[0013] 本发明提供的检测方法， 简单实用， 成本低廉， 可快速的筛査出前列腺液中不含游 离血红素的样本、 含游离血红素的样本和可能含有游离血红素的样本， 为进一歩检查做准 备； 本发明可检测出的最低游离血红素浓度可达 0.5ng/L，， 灵敏度高； 同时本发明还提供了 - 种 列腺液取样套， 可专用于采集 ^列腺液； 同时本发明还提供了一种检测盒， 可专用来 盛放检测过程中用到的器皿和试剂， 本试剂盒结构简单， 只需一个试剂盒便能完成整个检测 歩骤， 节省空间和试剂， 同时也减轻操作者劳动负扔。
附图说明
[0014] 图 1为外包装盒的结构示意图。
[0015] 图 2为盒体的结构示意图。
[0016] 图 3为 列腺液取样套的结构示意图。
[0017] 图中， 1.外包装盒； 2.比色板； 3.盒体； 4.镊子存放 ; 5.样品杯存放区； 6.滴管存放 区； 7.测试池； 8.前列腺液取样套； 9.小囊。
具体实施方式
[0018] 卜面结合实施例对本发明作进一 的说明。
说 明 书
[0019]
实施例 1
[0021] 使受检者取膝胸位， 打开检测盒的外包装盒 1， 从盒体 3内取出前列腺液取样套 8， 套在受检者阴茎龟头部位超过冠状沟。 检查者左手扶持患者左肩或臀部， 右手戴手套或指 套， 指端涂凡士林或液体石蜡， 食指先在肛门口轻轻按摩， 然后将手指徐徐插入肛门， 当指 端进入距肛门门约 5cm直肠前壁处即可触及前列腺， 以手指末节作向内、 向前徐徐按摩， 每侧约 4 5次， 然后再将手移至腺体的上部顺正中沟向下挤压， 这样前列腺液即可山尿道 排出至前列腺液取样套 8上， 留取标本送检。 其中， 所取前列腺液量为 0.5-1 ml; 取样品杯 放置区 5的样品杯内预装有 PH值为 7.4的三羟甲基氨基甲烷 -盐酸缓冲液 3 ml, 把带有前列 腺液的前列腺取样套 8放入样品杯中， 混匀； 从滴管存放区 6内取出两支滴管， 两支滴管分 别预装有试剂 A和试剂 B , 在测试池 7内顺序加入样品液 lml， 试剂 A 0.5ml， 试剂 B 0.5ml, 混匀， 2分钟内观察颜色变化， 与检测盒外包装的比色板 2比对 i己录检测结果； 所述 试剂 A的配制方法如下： 取 3,3',5，5'-四甲基联苯胺 50mg溶于 PH4.4柠檬酸缓冲液 100ml混 匀而成； 所述试剂 B为 3%的过氧化氢溶液。
[0020] 检测结果判定如下：
若不显色则为阴性， 说明渗液中无游离血红素； 若显示浅绿蓝色至浅蓝色， 可能含有游离血 红素， 需定期进行复査； 若显示蓝色至深蓝色则说明渗液中含有游离血红素。 若即刻显示深 蓝色且瞬间转为深黄色或棕红色， 预示有肿瘤组织出血， 亦应视为强阳性。
[0021] 实验资料：
分别取检测为不显色、 显示浅绿蓝色至浅蓝色、 蓝色至深蓝色的样品各二例， 用质谱分析仪 进行血红素的检测， 检测结果如下： 不显色均未测及血红素； 浅绿色至浅蓝色为： 0.5 ng/L ， 1.7ng/L 、 4.3 ng/L ; 蓝色至深蓝色为 20ng/L、 2.5 g/L、 52 μ g/L 。
[0022] 通过以上数据可以看出， 对游离血红素含量在 0.5ng/L以上的渗液本检测方法就可以 初步的判断， 游离血红素含量在 20ng/L以上的渗液本检测方法就可以确定为渗液中含有游 离血红素， 所以本检测方法的灵敏度高。
[0023] 实施例 2
- -种检测 列腺游离血红素的检测盒， 包括盒体 3和盒体外的外包装盒 1， 所述外包装盒 1 的外表面设有比色板 2， 所述盒体设冇向内 陷而成的镊子存放 Lx: 4、 样品杯存放 5、 滴 管存放区 6和测试池 7， 所述测试池 7内存放冇前列腺液取样套 8， 所述前列腺液取样套 8 呈底端封闭的圆简状， 所述底端还设有 Ί"ι起的小囊 9， 所述 列腺液取样套 8材质为吸附
说 明 书
性无紡布， 所述前列腺液取样套 8的直径 3.5cm， 长度 5cm， 所述小囊 9直径 lcm， 深度 0.5cm, 所述测试池 7设于盒体 3中上方， 直径 1.8cm， 深度 1.2cm， 所述滴管存放区 6设于 测试池 7两侧， 所述样品杯存放区 5设于测试池 7左侧， 所述镊子放置区 4设于样品杯存放 区 5左侧， 所述滴管存放区 6内设有滴管， 滴管上带有刻度， 所述样品杯放置区 5内设有样 品杯， 所述样品杯设有密封盖， 样品杯的杯体呈圆筒状， 所述镊子存放区 4内设有镊子， 所 述镊子为塑料镊。
Claims
1. 一种检测前列腺液游离血红素的方法， 包括以下检测歩骤：
1) 使受检者取膝胸位， 打开检测盒的外包装盒， 从盒体内取出前列腺液取样套， 套在受 检者阴茎龟头部位超过冠状沟， 检查者左手扶持患者左肩或臀部， 右手戴手套或指套， 指端 涂凡士林或液体石蜡； 食指先在肛门口轻轻按摩， 然后将手指徐徐插入肛门， 当指端进入距 肛门门约 5cm直肠前壁处即可触及前列腺， 以手指末节作向内、 向前徐徐按摩， 每侧约 4-5 次， 然后再将手移至腺体的上部顺正中沟向下挤压， 这样前列腺液即可 ώ尿道排出至前列腺 液取样套上， 留取标本送检； 其中， 所取前列腺液量为 0.5-1 ml;
2) 把带有前列腺液的前列腺液取样套放入样品杯内， 样品杯内预装有三羟甲基氨基甲烷- 盐酸缓冲液， 缓冲液的量为 3ml， 混匀， 得样品液； 3 ) 在测试池内顺序加入样品液 lml， 试剂 A 0.5ml， 试剂 B 0.5ml; 混匀， 2分钟内观察颜色变化， 与比色板比对记录检测结果； 所述试剂 A的配制方法如下： 取 3,3',5,5'-四甲基联苯胺 50mg溶于 PH为 7.4柠檬酸缓冲液 100ml混匀而成； 所述试剂 B为 3%的过氧化氢溶液。
2. 根据权利要求 1所述的检测前列腺液游离血红素的方法， 其特征在于， 所述的三羟甲基 氨基甲烷-盐酸缓冲液的 PH值为 7.4 。
3. ·—种权利要求 1或 2所述的检测前列腺液游离血红素的方法的检测盒， 其特征在于， 包 括盒体和盒体外的外包装盒， 所述外包装盒的外表面设有比色板， 所述盒体设有向内凹陷而 成的镊子存放区、 样品杯存放区、 滴管存放区和测试池， 所述测试池内存放有前列腺液取样 套， 所述前列腺液取样套呈底端封闭的圆筒状， 所述底端还设有一凸起的小囊。
4. 根据权利要求 3所述的检测盒， 其特征在于， 所述前列腺取样套材质为吸附性无紡布。
5. 根据权利要求 4所述的检测盒， 其特征在于， 所述前列腺液取样套的直径 3.5cm， 长度 5cm, 所述小囊直径 lcm， 深度 0.5cm。
6. 根据权利要求 3所述的检测盒， 其特征在于， 所述测试池设于测试盒中上方， 直径 1.8cm, 深度 1.2cm。
7. 根据权利要求 3所述的检测盒， 其特征在于， 所述滴管存放区设于测试池两侧， 所述样 品杯存放 K设于测试池左侧， 所述镊子放置区设于样品杯存放区左侧。
8. 根据权利要求 3或 7所述的检测盒， 其特征在于， 所述滴管存放 内设有滴管， 滴管上 带有刻度。
9. 根据权利要求 3所述的检测盒， 其特征在于， 所述样品杯放置区内设有样品杯， 所述样 品杯设有密封盖， 样品杯的杯体呈圆筒状。
10. 根据权利要求 3所述的检测盒， π特征在 J:， 所述镊丫 放 内设冇镊了'， 所述镊子为
权 利 要 求 书 塑料镊。
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| WO2005106498A1 (en) * | 2004-04-30 | 2005-11-10 | Enterix Pty. Limited | Device and method for detecting the presence of hemoglobin in a biological sample |
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