WO2013082542A3 - Procédés de biosynthèse du 1,3-butadiène - Google Patents

Procédés de biosynthèse du 1,3-butadiène Download PDF

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Publication number
WO2013082542A3
WO2013082542A3 PCT/US2012/067463 US2012067463W WO2013082542A3 WO 2013082542 A3 WO2013082542 A3 WO 2013082542A3 US 2012067463 W US2012067463 W US 2012067463W WO 2013082542 A3 WO2013082542 A3 WO 2013082542A3
Authority
WO
WIPO (PCT)
Prior art keywords
3butadiene
biosynthesizing
methods
butadiene
pathways
Prior art date
Application number
PCT/US2012/067463
Other languages
English (en)
Other versions
WO2013082542A2 (fr
Inventor
Paul S. Pearlman
Changlin Chen
Adriana BOTES
Alex van Eck CONRADIE
Original Assignee
Invista North America S.A.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invista North America S.A.R.L. filed Critical Invista North America S.A.R.L.
Priority to CN201280068870.1A priority Critical patent/CN104321434A/zh
Priority to BR112014012999A priority patent/BR112014012999A2/pt
Priority to EP12799032.3A priority patent/EP2785848A2/fr
Publication of WO2013082542A2 publication Critical patent/WO2013082542A2/fr
Priority to CN201380043586.3A priority patent/CN104769119A/zh
Priority to PCT/US2013/045430 priority patent/WO2013188546A2/fr
Priority to JP2015517396A priority patent/JP2015519083A/ja
Priority to EP13739305.4A priority patent/EP2861745A2/fr
Priority to IN309DEN2015 priority patent/IN2015DN00309A/en
Publication of WO2013082542A3 publication Critical patent/WO2013082542A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01033—Diphosphomevalonate decarboxylase (4.1.1.33), i.e. mevalonate-pyrophosphate decarboxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des voies biochimiques pour produire du butadiène par formation de deux groupes vinyle dans un substrat de synthèse de butadiène. Ces voies selon l'invention utilisent des enzymes telles que le mévalonate diphosphate décarboxylase, l'isoprène synthase et les déshydratases dans l'étape enzymatique finale.
PCT/US2012/067463 2011-06-17 2012-11-30 Procédés de biosynthèse du 1,3-butadiène WO2013082542A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CN201280068870.1A CN104321434A (zh) 2011-12-02 2012-11-30 生物合成1,3丁二烯的方法
BR112014012999A BR112014012999A2 (pt) 2011-12-02 2012-11-30 método para biossíntese de butadieno
EP12799032.3A EP2785848A2 (fr) 2011-12-02 2012-11-30 Procédés de biosynthèse du 1,3-butadiène
CN201380043586.3A CN104769119A (zh) 2012-06-15 2013-06-12 生物合成1,3丁二烯的方法
PCT/US2013/045430 WO2013188546A2 (fr) 2012-06-15 2013-06-12 Procédés pour la biosynthèse de 1,3-butadiène
JP2015517396A JP2015519083A (ja) 2012-06-15 2013-06-12 １，３−ブタジエンを生合成するための方法
EP13739305.4A EP2861745A2 (fr) 2012-06-15 2013-06-12 Procédés pour la biosynthèse de 1,3-butadiène
IN309DEN2015 IN2015DN00309A (fr) 2011-06-17 2013-06-12

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161566085P 2011-12-02 2011-12-02
US61/566,085 2011-12-02
US201261714883P 2012-10-17 2012-10-17
US61/714,883 2012-10-17

Publications (2)

Publication Number Publication Date
WO2013082542A2 WO2013082542A2 (fr) 2013-06-06
WO2013082542A3 true WO2013082542A3 (fr) 2013-08-22

Family

ID=47326427

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/067463 WO2013082542A2 (fr) 2011-06-17 2012-11-30 Procédés de biosynthèse du 1,3-butadiène

Country Status (4)

Country Link
EP (1) EP2785848A2 (fr)
CN (1) CN104321434A (fr)
BR (1) BR112014012999A2 (fr)
WO (1) WO2013082542A2 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9422578B2 (en) 2011-06-17 2016-08-23 Invista North America S.A.R.L. Methods for biosynthesizing 1,3 butadiene
WO2012174439A2 (fr) 2011-06-17 2012-12-20 Invista Technologies S.A R.L. Procédés d'obtention de molécules à quatre carbones
WO2012174451A1 (fr) 2011-06-17 2012-12-20 Invista Technologies S.A.R.L. Utilisation d'hydrolases pour augmenter la teneur en monomères dans un flux de déchets
JP2014525741A (ja) 2011-06-30 2014-10-02 インビスタ テクノロジーズ エス．アー．エール．エル． ナイロン−７、ナイロン−７，７、およびポリエステルを生産するための生物変換方法
US9102958B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
US9102960B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
AU2012357857B2 (en) * 2011-12-20 2016-01-21 Scientist Of Fortune S.A. Production of 1,3-dienes by enzymatic conversion of 3-hydroxyalk-4-enoates and/or 3-phosphonoxyalk-4-enoates
CN104769119A (zh) * 2012-06-15 2015-07-08 英威达技术有限责任公司 生物合成1,3丁二烯的方法
US8703455B2 (en) 2012-08-29 2014-04-22 Scientist of Fourtune, S.A. Production of volatile dienes by enzymatic dehydration of light alkenols
WO2014085612A1 (fr) 2012-11-28 2014-06-05 INVISTA North America S.á r.l. Procédés de biosynthèse d'isobutène
US9790525B2 (en) 2012-12-14 2017-10-17 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
EP2938736A2 (fr) 2012-12-31 2015-11-04 Invista Technologies S.A R.L. Procédés de production d'agents chimiques à 7 atomes de carbone par allongement de la chaîne carbonée c1 associé à la synthèse de la coenzyme b
CN105026569A (zh) 2012-12-31 2015-11-04 英威达技术有限责任公司 通过丙酮酸和琥珀酸半醛羟醛缩合生产7-碳化学物的方法
CN105008543A (zh) 2012-12-31 2015-10-28 英威达技术有限责任公司 通过芳香族化合物生产7-碳化学物的方法
US9920336B2 (en) 2012-12-31 2018-03-20 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals from long chain fatty acids via oxidative cleavage
WO2014105788A2 (fr) 2012-12-31 2014-07-03 Invista North America S.A.R.L. Procédés de production d'agents chimiques à 7 atomes de carbone par allongement de chaîne carbone associé à la synthèse de cyclohexane carboxylate
EP2938733A2 (fr) 2012-12-31 2015-11-04 Invista North America S.a.r.l. Procédés de production d'agents chimiques par l'allongement de chaîne carbonée protégée par de l'ester méthylique
CN105073214A (zh) 2012-12-31 2015-11-18 英威达技术有限责任公司 通过甲酯保护的碳链延伸生产6碳化学物的方法
EP3017051B1 (fr) 2013-07-03 2021-06-30 Scientist of Fortune S.A. Procédé de production enzymatique de 3-butèn-2-one
US10294496B2 (en) 2013-07-19 2019-05-21 Invista North America S.A.R.L. Methods for biosynthesizing 1,3 butadiene
BR112016002104A2 (pt) * 2013-07-29 2017-08-01 Invista Tech Sarl método de síntese de 3-oxopent-4-enoil-coa
CN105683385A (zh) 2013-08-05 2016-06-15 英威达技术有限责任公司 用于生物合成异戊二烯的方法
CN105683384A (zh) 2013-08-05 2016-06-15 英威达技术有限责任公司 用于生物合成异丁烯的方法
WO2015031496A1 (fr) * 2013-08-27 2015-03-05 Invista North America S.A.R.L. Procédés et matériels pour la production d'éléments structuraux à cinq atomes de carbone à partir de proline
BR112016026461A2 (pt) 2014-05-15 2017-12-12 Invista Tech Sarl métodos de produção de produtos químicos com 6 carbonos com uso de 2,6-diaminopimelato como precursor para 2-aminopimelato
BR112016029375A2 (pt) 2014-06-16 2017-10-17 Invista Tech Sarl métodos, reagentes e células para biossintetizar compostos
CN106795535A (zh) 2014-06-16 2017-05-31 英威达技术有限责任公司 用于生物合成化合物的方法，试剂和细胞
EP3155106A2 (fr) 2014-06-16 2017-04-19 Invista Technologies S.à.r.l. Procédés, réactifs et cellules utilisables en vue de la biosynthèse d'un composé
CN106795519A (zh) 2014-06-16 2017-05-31 英威达技术有限责任公司 用于生成戊二酸和戊二酸甲酯的方法
US10214752B2 (en) * 2014-11-14 2019-02-26 Invista North America S.A.R.L. Biosynthesis of 1,3-butanediol
US10801050B2 (en) 2015-10-23 2020-10-13 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
US11286490B2 (en) 2016-07-12 2022-03-29 Braskem S.A. Formation of alkenes through enzymatic dehydration of alkanols
WO2019006255A1 (fr) 2017-06-30 2019-01-03 Invista North America S.A.R.L. Procédés, matériaux, hôtes synthétiques et réactifs pour la biosynthèse d'hydrocarbures et leurs dérivés
US11162115B2 (en) 2017-06-30 2021-11-02 Inv Nylon Chemicals Americas, Llc Methods, synthetic hosts and reagents for the biosynthesis of hydrocarbons
US11505809B2 (en) 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis
CN108690851B (zh) * 2018-05-30 2020-11-06 青岛农业大学 一种丁二烯生产菌及其生产丁二烯的方法
CN109097409A (zh) * 2018-08-10 2018-12-28 浙江正硕生物科技有限公司 D-氨基酸和阿尔法酮酸的制备方法
CN110305856B (zh) * 2019-06-27 2020-12-01 华中农业大学 一种细胞色素p450酶的应用
CN114107141B (zh) * 2021-08-19 2022-07-12 中国科学院天津工业生物技术研究所 高产l-脯氨酸的谷氨酸棒杆菌以及高产l-脯氨酸的方法

Citations (3)

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WO2009155382A1 (fr) * 2008-06-17 2009-12-23 Genomatica, Inc. Micro-organismes et procédés pour la biosynthèse de fumarate, malate, et acrylate
WO2011140171A2 (fr) * 2010-05-05 2011-11-10 Genomatica, Inc. Micro-organismes et procédés pour la biosynthèse de butadiène
US20120021478A1 (en) * 2010-07-26 2012-01-26 Osterhout Robin E Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene

Family Cites Families (2)

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Publication number Priority date Publication date Assignee Title
AU2012357857B2 (en) * 2011-12-20 2016-01-21 Scientist Of Fortune S.A. Production of 1,3-dienes by enzymatic conversion of 3-hydroxyalk-4-enoates and/or 3-phosphonoxyalk-4-enoates
CN104769119A (zh) * 2012-06-15 2015-07-08 英威达技术有限责任公司 生物合成1,3丁二烯的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009155382A1 (fr) * 2008-06-17 2009-12-23 Genomatica, Inc. Micro-organismes et procédés pour la biosynthèse de fumarate, malate, et acrylate
WO2011140171A2 (fr) * 2010-05-05 2011-11-10 Genomatica, Inc. Micro-organismes et procédés pour la biosynthèse de butadiène
US20110300597A1 (en) * 2010-05-05 2011-12-08 Burk Mark J Microorganisms and methods for the biosynthesis of butadiene
US20120021478A1 (en) * 2010-07-26 2012-01-26 Osterhout Robin E Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene

Also Published As

Publication number Publication date
WO2013082542A2 (fr) 2013-06-06
BR112014012999A2 (pt) 2017-06-13
EP2785848A2 (fr) 2014-10-08
CN104321434A (zh) 2015-01-28

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