WO2013022203A3 - 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법 - Google Patents

인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법 Download PDF

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Publication number
WO2013022203A3
WO2013022203A3 PCT/KR2012/005971 KR2012005971W WO2013022203A3 WO 2013022203 A3 WO2013022203 A3 WO 2013022203A3 KR 2012005971 W KR2012005971 W KR 2012005971W WO 2013022203 A3 WO2013022203 A3 WO 2013022203A3
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Prior art keywords
nucleic acid
metal nanoparticles
intercalator
present
conjugated
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PCT/KR2012/005971
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English (en)
French (fr)
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WO2013022203A2 (ko
WO2013022203A9 (ko
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정봉현
김상규
조현민
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인텔렉추얼디스커버리 주식회사
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Application filed by 인텔렉추얼디스커버리 주식회사 filed Critical 인텔렉추얼디스커버리 주식회사
Priority to EP12822084.5A priority Critical patent/EP2740802A4/en
Priority to US14/237,324 priority patent/US20140295567A1/en
Priority to CN201280038262.6A priority patent/CN103717754B/zh
Publication of WO2013022203A2 publication Critical patent/WO2013022203A2/ko
Publication of WO2013022203A9 publication Critical patent/WO2013022203A9/ko
Publication of WO2013022203A3 publication Critical patent/WO2013022203A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법에 관한 것으로, 보다 구체적으로, 기판 상에 DNA 프로브가 고정된 DNA칩에 목적핵산을 포함하는 시료를 적용시킨 다음, 이중나선 핵산과 결합하는 인컬레이터가 컨쥬게이트된 금속 나노입자를 반응시킨 후 금속 인핸싱 용액(enhancing solution)을 반응시켜서 상기 금속 나노입자의 크기를 증폭하여 육안으로 목적핵산을 검출하는 방법에 관한 것이다. 본 발명에 따른 핵산의 검출방법은 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용함으로써, 다른 어떠한 장비 없이도 육안으로 핵산 검출이 가능하여 기존의 검출 방식보다 검출 시간 및 분석 비용이 절감되는 효과뿐만 아니라 장치의 소형화가 가능하여 가축 농장이나 가정 등에서 현장 진단이 가능하다는 장점이 있다.
PCT/KR2012/005971 2011-08-05 2012-07-26 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법 WO2013022203A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP12822084.5A EP2740802A4 (en) 2011-08-05 2012-07-26 METHOD OF DETECTING NUCLEIC ACID USING METAL NANOPARTICLES CONJUGATED TO AN INTERCALATOR
US14/237,324 US20140295567A1 (en) 2011-08-05 2012-07-26 Method for detecting nucleic acid using intercalator-conjugated metal nanoparticles
CN201280038262.6A CN103717754B (zh) 2011-08-05 2012-07-26 使用与嵌入剂偶联的金属纳米粒子检测核酸的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0078052 2011-08-05
KR1020110078052A KR101397793B1 (ko) 2011-08-05 2011-08-05 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법

Publications (3)

Publication Number Publication Date
WO2013022203A2 WO2013022203A2 (ko) 2013-02-14
WO2013022203A9 WO2013022203A9 (ko) 2013-04-04
WO2013022203A3 true WO2013022203A3 (ko) 2013-06-13

Family

ID=47669037

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/005971 WO2013022203A2 (ko) 2011-08-05 2012-07-26 인터컬레이터가 컨쥬게이트된 금속 나노입자를 이용한 핵산의 검출방법

Country Status (5)

Country Link
US (1) US20140295567A1 (ko)
EP (1) EP2740802A4 (ko)
KR (1) KR101397793B1 (ko)
CN (1) CN103717754B (ko)
WO (1) WO2013022203A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015123654A1 (en) * 2014-02-17 2015-08-20 The Cleveland Clinic Foundation Amine passivated nanoparticles for cancer treatment and imaging
US11293055B2 (en) * 2015-07-13 2022-04-05 National Cancer Center Nucleic acid detection kit and nucleic acid detection method using nanoparticles
KR102082117B1 (ko) * 2018-03-30 2020-02-27 한국과학기술원 육안으로 판독 가능한 입자 기반 유전자 측정 방법 및 시스템
KR102242704B1 (ko) * 2019-01-31 2021-04-21 한국과학기술원 모바일 기기와 통합된 병원체의 진단 시스템

Citations (4)

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Publication number Priority date Publication date Assignee Title
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
KR100498292B1 (ko) * 2003-02-06 2005-07-01 엘지전자 주식회사 경사각이 형성된 구멍을 갖는 핵산 칩을 이용하는 핵산혼성화 검출기 및 핵산 혼성화 검출방법
KR20070060592A (ko) * 2005-12-09 2007-06-13 래플진(주) 금속 나노 교상체 및 인터컬레이터를 이용한 핵산의검출방법
KR101048429B1 (ko) * 2009-09-09 2011-07-11 한국생명공학연구원 바이오칩을 이용한 표적 물질 검출 및 정량 방법

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US20100291707A1 (en) * 2009-04-29 2010-11-18 Northwestern University Multiplexed Scanometric Assay for Target Molecules
CN103154735B (zh) * 2009-05-11 2018-10-02 连接Dx股份有限公司 用于检测分析物的方法和组合物

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
KR100498292B1 (ko) * 2003-02-06 2005-07-01 엘지전자 주식회사 경사각이 형성된 구멍을 갖는 핵산 칩을 이용하는 핵산혼성화 검출기 및 핵산 혼성화 검출방법
KR20070060592A (ko) * 2005-12-09 2007-06-13 래플진(주) 금속 나노 교상체 및 인터컬레이터를 이용한 핵산의검출방법
KR101048429B1 (ko) * 2009-09-09 2011-07-11 한국생명공학연구원 바이오칩을 이용한 표적 물질 검출 및 정량 방법

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CHO, H. ET AL.: "Scanometric analysis of DNA microarrays using DNA intercalator- conjugated gold nanoparticles", CHEM. COMMUN. (CAMB)., vol. 48, no. 61, 7 August 2012 (2012-08-07), pages 7601 - 7603, XP055071199 *

Also Published As

Publication number Publication date
KR20130015810A (ko) 2013-02-14
CN103717754A (zh) 2014-04-09
EP2740802A4 (en) 2015-03-25
WO2013022203A2 (ko) 2013-02-14
CN103717754B (zh) 2016-03-09
EP2740802A2 (en) 2014-06-11
US20140295567A1 (en) 2014-10-02
KR101397793B1 (ko) 2014-05-27
WO2013022203A9 (ko) 2013-04-04

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