WO2012161522A3 - Ｌ-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법 - Google Patents

Ｌ-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법 Download PDF

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WO2012161522A3
WO2012161522A3 PCT/KR2012/004089 KR2012004089W WO2012161522A3 WO 2012161522 A3 WO2012161522 A3 WO 2012161522A3 KR 2012004089 W KR2012004089 W KR 2012004089W WO 2012161522 A3 WO2012161522 A3 WO 2012161522A3
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ornithine
microorganism
preparing
production
present
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WO2012161522A2 (ko
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조재용
고영웅
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(주)강원지역대학연합기술지주회사
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Publication of WO2012161522A2 publication Critical patent/WO2012161522A2/ko
Publication of WO2012161522A3 publication Critical patent/WO2012161522A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/9901—Glucose dehydrogenase (acceptor) (1.1.99.10)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 L-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 L-오르니틴의 제조방법에 관한 것으로, 본 발명은 보다 구체적으로 내재적 글루코스 디하이드로게나제(GDH)의 활성이 감소 또는 불활성화되어, L-오르니틴 생산능이 향상된 코리네박테리움 속 미생물, 상기 미생물의 제조방법 및 상기 미생물을 글루코스를 탄소원으로 사용하여 배양하고, 상기 배양물로부터 L-오르니틴을 회수하는 단계를 포함하는 L-오르니틴의 제조방법에 관한 것이다. 본 발명의 내재적 글루코스 디하이드로게나제 효소의 활성이 감소된 코리네박테리움 속 미생물을 이용하면, 오탄당 인산화 경로가 활성화될 뿐만 아니라, 오탄당 인산화 경로에 관여하는 효소인 G6PDH와 6PGD의 특이적 활성의 증대에 의하여 NADPH 생산수율이 증가하고, 이에 따라 상기 NADPH를 이용한 L-오르니틴의 생산수율을 향상시킬 수 있으므로, L-오르니틴의 효율적이고 경제적인 생산에 널리 활용될 수 있을 것이다.
PCT/KR2012/004089 2011-05-23 2012-05-23 Ｌ-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법 WO2012161522A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110048623A KR101300186B1 (ko) 2011-05-23 2011-05-23 Ｌ-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법
KR10-2011-0048623 2011-05-23

Publications (2)

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WO2012161522A2 WO2012161522A2 (ko) 2012-11-29
WO2012161522A3 true WO2012161522A3 (ko) 2013-01-24

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PCT/KR2012/004089 WO2012161522A2 (ko) 2011-05-23 2012-05-23 Ｌ-오르니틴 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법

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KR (1) KR101300186B1 (ko)
WO (1) WO2012161522A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103014086A (zh) * 2012-12-12 2013-04-03 武汉圣佳化工有限公司 一种酶固定化连续生产l-鸟氨酸复合盐的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101526047B1 (ko) * 2013-04-26 2015-06-04 상지대학교산학협력단 아미노트랜스퍼라제 활성 강화를 통하여 ｌ-오르니틴 생산능이 향상된 미생물 및 이를 이용한 ｌ-오르니틴의 제조방법
KR101835935B1 (ko) 2014-10-13 2018-03-12 씨제이제일제당 (주) L-아르기닌을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아르기닌의 제조 방법
WO2016060437A1 (ko) * 2014-10-13 2016-04-21 씨제이제일제당 주식회사 L-아르기닌을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아르기닌의 제조 방법

Citations (3)

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KR20070056805A (ko) * 2005-11-30 2007-06-04 씨제이 주식회사 Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 ｌ-라이신을 생산하는 방법
WO2010027045A1 (ja) * 2008-09-08 2010-03-11 味の素株式会社 Ｌ－アミノ酸を生産する微生物及びｌ－アミノ酸の製造法
EP2218789A1 (en) * 2004-03-18 2010-08-18 Evonik Degussa GmbH Process for the production of L-amino acids using coryneform bacteria

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* Cited by examiner, † Cited by third party
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EP2218789A1 (en) * 2004-03-18 2010-08-18 Evonik Degussa GmbH Process for the production of L-amino acids using coryneform bacteria
KR20070056805A (ko) * 2005-11-30 2007-06-04 씨제이 주식회사 Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 ｌ-라이신을 생산하는 방법
WO2010027045A1 (ja) * 2008-09-08 2010-03-11 味の素株式会社 Ｌ－アミノ酸を生産する微生物及びｌ－アミノ酸の製造法

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DATABASE GENBANK 24 September 2009 (2009-09-24), accession no. P531537.1 *
DATABASE GENBANK 7 September 2010 (2010-09-07), accession no. H735883.1 *
HWANG, GWI HYE ET AL., APPLIED ANIMAL SCIENCE, THESIS FOR MASTER COURSE, June 2009 (2009-06-01) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103014086A (zh) * 2012-12-12 2013-04-03 武汉圣佳化工有限公司 一种酶固定化连续生产l-鸟氨酸复合盐的方法
CN103014086B (zh) * 2012-12-12 2015-02-11 武汉圣佳化工有限公司 一种酶固定化连续生产l-鸟氨酸复合盐的方法

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KR20120130580A (ko) 2012-12-03
WO2012161522A2 (ko) 2012-11-29

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