WO2012047015A3 - 핵산 압타머 선별을 위한 멀티플렉스 미세 유체 장치 및 이를 이용한 핵산 압타머의 고속 선별방법 - Google Patents

핵산 압타머 선별을 위한 멀티플렉스 미세 유체 장치 및 이를 이용한 핵산 압타머의 고속 선별방법 Download PDF

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WO2012047015A3
WO2012047015A3 PCT/KR2011/007367 KR2011007367W WO2012047015A3 WO 2012047015 A3 WO2012047015 A3 WO 2012047015A3 KR 2011007367 W KR2011007367 W KR 2011007367W WO 2012047015 A3 WO2012047015 A3 WO 2012047015A3
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nucleic acid
acid aptamers
high throughput
multiplex
microfluidic device
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PCT/KR2011/007367
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French (fr)
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WO2012047015A2 (ko
WO2012047015A9 (ko
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김소연
안지영
조민정
김태경
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피씨엘 (주)
동국대학교 산학협력단
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Priority to CN201180004708.9A priority Critical patent/CN102639720B/zh
Priority to US13/877,862 priority patent/US10221412B2/en
Publication of WO2012047015A2 publication Critical patent/WO2012047015A2/ko
Publication of WO2012047015A3 publication Critical patent/WO2012047015A3/ko
Publication of WO2012047015A9 publication Critical patent/WO2012047015A9/ko

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Abstract

본 발명은 핵산 압타머 선별을 위한 멀티플렉스 미세 유체 장치 및 이를 이용한 핵산 압타머의 고속 선별방법에 관한 것으로, 보다 구체적으로는 기존 단일 타겟에 대한 압타머 개발방식을 개선된 멀티플렉스 플랫폼으로 전환시킨 멀티플렉스 마이크로 유체 장치 (SELEX lap-on-a-chip) 및 이를 고속 시퀀싱법(high throughput sequencing)과 연계한 것을 특징으로 하는 핵산 압타머의 고속 선별방법(high throughput selection mehtod)에 관한 것이다. 본 발명에 따른 멀티플렉스 유체 장치는 다수개의 타겟에 대한 각 압타머를 동시에 검출할 수 있을 뿐만 아니라, 종래의 멀티플렉스 기법에 비하여 처리량 및 처리시간을 크게 감축하는 장점이 있으며, 특히 본 발명에 따른 장치를 고속 시퀀싱법과 연계하여 압타머 선별공정을 수행함으로써 타겟결합/분리/증폭 공정 라운드를 대폭 감소시키고 자동화 공정의 수행을 가능하게 하는바, 매우 유용하다.
PCT/KR2011/007367 2010-10-05 2011-10-05 핵산 압타머 선별을 위한 멀티플렉스 미세 유체 장치 및 이를 이용한 핵산 압타머의 고속 선별방법 WO2012047015A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201180004708.9A CN102639720B (zh) 2010-10-05 2011-10-05 用于筛选核酸适配子的多元微流体装置及利用此装置的核酸适配子的高通量筛选方法
US13/877,862 US10221412B2 (en) 2010-10-05 2011-10-05 Multiplex microfluidic device for selecting nucleic acid aptamers, and high throughput selection method for nucleic acid aptamers using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100096685 2010-10-05
KR10-2010-0096685 2010-10-05

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WO2012047015A2 WO2012047015A2 (ko) 2012-04-12
WO2012047015A3 true WO2012047015A3 (ko) 2012-06-21
WO2012047015A9 WO2012047015A9 (ko) 2012-08-09

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US (1) US10221412B2 (ko)
KR (1) KR101423032B1 (ko)
CN (1) CN102639720B (ko)
WO (1) WO2012047015A2 (ko)

Families Citing this family (14)

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WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
DE102011085473A1 (de) * 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren
CN103278643B (zh) * 2013-05-16 2015-05-27 中国科学院上海微系统与信息技术研究所 一种用于微量蛋白检测的微芯片的制备方法
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
KR101849557B1 (ko) * 2014-09-29 2018-04-17 동국대학교 산학협력단 신규 세포 타겟 압타머 제조방법
US20190091689A1 (en) * 2014-10-17 2019-03-28 Ecole Polytechnique Federale De Lausanne (Epfl) Microfluidic Device and Method for Isolation of Nucleic Acid
US10546650B2 (en) * 2015-10-23 2020-01-28 Google Llc Neural network for processing aptamer data
US10882045B2 (en) * 2016-01-08 2021-01-05 Hewlett-Packard Development Company, L.P. Polymerase chain reaction device including ejection nozzles
CN109694806B (zh) * 2017-10-24 2021-11-19 湖南大学 一种微流体装置及基于微流体装置的单细胞核酸适体单轮筛选方法
TWI675106B (zh) * 2018-03-21 2019-10-21 緯創資通股份有限公司 液體處理模組、液體檢測系統及利用液體處理模組進行檢測的檢測方法
CN110564815A (zh) * 2019-09-03 2019-12-13 集美大学 一种筛选获得极短序列核酸适配体的方法
KR102414945B1 (ko) * 2019-10-02 2022-07-05 한국식품연구원 핵산 선별용 졸-겔 나노 필터 및 이를 이용한 핵산 선별 방법
BR102020004436A8 (pt) 2020-03-05 2022-11-22 Bioptamers Inc Dispositivo microfluídico
US11845079B2 (en) * 2021-12-08 2023-12-19 International Business Machines Corporation Integrated silicon platform for electronic biosensors

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WO2010019969A1 (en) * 2008-08-15 2010-02-18 Cornell University Device for rapid identification of nucleic acids for binding to specific chemical targets

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US20130274113A1 (en) 2013-10-17
US10221412B2 (en) 2019-03-05
KR101423032B1 (ko) 2014-07-28
WO2012047015A2 (ko) 2012-04-12
CN102639720B (zh) 2015-03-18
WO2012047015A9 (ko) 2012-08-09
CN102639720A (zh) 2012-08-15
KR20120035893A (ko) 2012-04-16

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