BR102020004436A8 - Dispositivo microfluídico - Google Patents

Dispositivo microfluídico

Info

Publication number
BR102020004436A8
BR102020004436A8 BR102020004436A BR102020004436A BR102020004436A8 BR 102020004436 A8 BR102020004436 A8 BR 102020004436A8 BR 102020004436 A BR102020004436 A BR 102020004436A BR 102020004436 A BR102020004436 A BR 102020004436A BR 102020004436 A8 BR102020004436 A8 BR 102020004436A8
Authority
BR
Brazil
Prior art keywords
selection
microfluid device
microfluid
selex
aptamers
Prior art date
Application number
BR102020004436A
Other languages
English (en)
Other versions
BR102020004436A2 (pt
Inventor
De Simone Molina Erika
Galves Moretto Emerson
Original Assignee
Bioptamers Inc
Bioptamers Ltda
Bioptamers Pesquisa E Desenvolvimento Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioptamers Inc, Bioptamers Ltda, Bioptamers Pesquisa E Desenvolvimento Ltda filed Critical Bioptamers Inc
Priority to BR102020004436A priority Critical patent/BR102020004436A8/pt
Priority to MX2022010876A priority patent/MX2022010876A/es
Priority to EP21764657.9A priority patent/EP4116433A1/en
Priority to US17/908,712 priority patent/US20230159988A1/en
Priority to PCT/BR2021/050096 priority patent/WO2021174327A1/pt
Priority to CN202180018915.3A priority patent/CN115244187A/zh
Publication of BR102020004436A2 publication Critical patent/BR102020004436A2/pt
Publication of BR102020004436A8 publication Critical patent/BR102020004436A8/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

DISPOSITIVO MICROFLUÍDICO. A presente invenção materializa-se em um dispositivo microfluídico inovador capaz de melhorar a seleção de ligantes de maior especificidade de bibliotecas de biomoléculas contra culturas de células ou tecidos humanos, especialmente durante a seleção de aptâmeros por SELEX.
BR102020004436A 2020-03-05 2020-03-05 Dispositivo microfluídico BR102020004436A8 (pt)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BR102020004436A BR102020004436A8 (pt) 2020-03-05 2020-03-05 Dispositivo microfluídico
MX2022010876A MX2022010876A (es) 2020-03-05 2021-03-05 Metodo para seleccionar aptameros con alta especificidad de diana en una plataforma de dispositivo microfluidico para el cocultivo de multiples tejidos.
EP21764657.9A EP4116433A1 (en) 2020-03-05 2021-03-05 Method for selecting aptamers with high target specificity in a microfluidic device platform for multiple tissue cocultivation
US17/908,712 US20230159988A1 (en) 2020-03-05 2021-03-05 Method for selecting aptamers with high target specificity in a microfluidic device platform for co-culture of multiple tissues
PCT/BR2021/050096 WO2021174327A1 (pt) 2020-03-05 2021-03-05 Método para seleção de aptâmeros com alta especificidade pelo alvo em plataforma de dispositivos microfluídicos para co-cultivo de tecidos múltiplos
CN202180018915.3A CN115244187A (zh) 2020-03-05 2021-03-05 多组织共培养微流体装置平台中筛选高靶向特异性适配体的方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR102020004436A BR102020004436A8 (pt) 2020-03-05 2020-03-05 Dispositivo microfluídico

Publications (2)

Publication Number Publication Date
BR102020004436A2 BR102020004436A2 (pt) 2022-04-26
BR102020004436A8 true BR102020004436A8 (pt) 2022-11-22

Family

ID=77612518

Family Applications (1)

Application Number Title Priority Date Filing Date
BR102020004436A BR102020004436A8 (pt) 2020-03-05 2020-03-05 Dispositivo microfluídico

Country Status (6)

Country Link
US (1) US20230159988A1 (pt)
EP (1) EP4116433A1 (pt)
CN (1) CN115244187A (pt)
BR (1) BR102020004436A8 (pt)
MX (1) MX2022010876A (pt)
WO (1) WO2021174327A1 (pt)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
JP5597633B2 (ja) 2008-08-15 2014-10-01 コーネル ユニバーシティ 化学的ターゲットと特異的に結合する核酸の迅速な確認のための装置
WO2011014674A2 (en) * 2009-07-29 2011-02-03 Cornell University Microfluidic device for pharmacokinetic-pharmacodynamic study of drugs and uses thereof
CN102639720B (zh) 2010-10-05 2015-03-18 Pcl公司 用于筛选核酸适配子的多元微流体装置及利用此装置的核酸适配子的高通量筛选方法
JP2012196197A (ja) 2011-03-04 2012-10-18 Kanagawa Acad Of Sci & Technol 標的細胞表面の標的分子に特異的に結合する核酸の選択法
CN102703454B (zh) 2012-06-27 2014-02-26 湖南大学 可用于检测肌红蛋白的核酸适体、筛选用微流控芯片及其筛选方法和应用
WO2018067802A1 (en) 2016-10-07 2018-04-12 The Regents Of The University Of Michigan Systems and methods for high throughput screening
CN106480039B (zh) 2016-10-13 2019-11-12 南京大学 一种利用微阵列微流控芯片筛选适配体的方法

Also Published As

Publication number Publication date
US20230159988A1 (en) 2023-05-25
BR102020004436A2 (pt) 2022-04-26
WO2021174327A1 (pt) 2021-09-10
MX2022010876A (es) 2022-11-07
EP4116433A1 (en) 2023-01-11
CN115244187A (zh) 2022-10-25

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Owner name: BIOPTAMERS INC (US)

Free format text: A FIM DE ATENDER A TRANSFERENCIA, REQUERIDA ATRAVES DA PETICAO NO 870210020781 DE 04/03/2021, E NECESSARIO APRESENTAR DOCUMENTO QUE COMPROVE QUE O REPRESENTANTE DA EMPRESA CEDENTE TEM PODERES PARA REALIZAR TAL ATO, ALEM DA GUIA DE CUMPRIMENTO DE EXIGENCIA.

B25C Requirement related to requested transfer of rights

Owner name: BIOPTAMERS INC (US)

Free format text: A FIM DE ATENDER A TRANSFERENCIA, REQUERIDA ATRAVES DA PETICAO NO 870210079710 DE 30/08/2021, E NECESSARIO APRESENTAR DOCUMENTO NOTARIZADO E COM APOSTILAMENTO OU LEGALIZACAO CONSULAR DA EMPRESA CEDENTE. ALEM DISSO, E PRECISO APRESENTAR A GUIA DE CUMPRIMENTO DE EXIGENCIA.

B25A Requested transfer of rights approved

Owner name: BIOPTAMERS LTDA (BR/SP)

B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B25F Entry of change of name and/or headquarter and transfer of application, patent and certif. of addition of invention: change of name on requirement

Owner name: BIOPTAMERS LTDA (BR/SP)

Free format text: A FIM DE ATENDER A ALTERACAO DE NOME REQUERIDA ATRAVES DA PETICAO NO 870220074268, DE 18/08/2022, E NECESSARIO APRESENTAR DOCUMENTO QUE EVIDENCIE A ALTERACAO DE NOME SOLICITADA. ALEM DISSO, E PRECISO APRESENTAR A GUIA DE CUMPRIMENTO DE EXIGENCIA.

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Owner name: BIOPTAMERS PESQUISA E DESENVOLVIMENTO LTDA (BR/SP)