WO2012026645A1

WO2012026645A1 - Method for treating and preventing sacbrood virus infection

Info

Publication number: WO2012026645A1
Application number: PCT/KR2010/006278
Authority: WO
Inventors: 정형범
Original assignee: Jung Hyeong Beom
Priority date: 2010-08-27
Filing date: 2010-09-14
Publication date: 2012-03-01

Abstract

The present invention relates to a method for treating and preventing sacbrood virus infection, and more particularly, to a method for treating and preventing sacbrood virus infection comprising the following steps: mixing wild ginseng and water at a weight ratio of 1:1-2 to obtain wild ginseng extract at a low temperature and under vacuum, then concentrating same; mixing the wild ginseng concentrate with water to manufacture liquid wild ginseng having a sugar content of at least 45 Brix; and diluting and providing the liquid wild ginseng to honeybees which have or have not contracted the sacbrood virus. According to the present invention, sacbrood virus infection can be effectively treated or prevented in Apis cerena or Apis mellifera.

Description

How to treat and prevent viral worms

The present invention relates to a method for treating and preventing Tobong virus worms.

Goat's ginseng is obtained by cultivating wild ginseng, which has been known to have similar efficacy. Among the 35 saponins contained in wild ginseng, the main roles of saponins medically revealed in Healthweek include Rb1-Liver Function Recovery / Fatigue Recovery, Rb2-Diabetes Control / Obesity and Atherosclerosis / Liver Proliferation, Rh1-Liver Function Improvement / Fatigue Restoration / platelet coagulation inhibition, Rh2-anticancer and immunity enhancement, Rg1-memory and dementia prevention / diabetes control / obesity prevention / fatigue prevention, Rg2-memory prevention and platelet coagulation inhibition, Rg3-anticancer and anticancer resistance / Immuno-enhancing, Re-Liver Protection / Promoting Bone Marrow Cell Production / Strengthening Memory and Preventing Dementia, Other Rf, Ro, Rd, etc.Let Lipid Peroxidation / Cerebral Nerve Cell Analgesic / Alcohol Detoxification, Inflammation Treatment / Protein and Lipid Synthesis / Corticosteroids secretion is promoted.

On the other hand, worms are caused by malnutrition of bees caused by the recent disappearance of flowers. Currently, more than 70% of Tobong farmers in Korea have a fatal blow to Tobong (Hanbong) bees domestically and globally. Tobong farmers are in serious danger due to the infection of “worm bugs” and the Ministry of Food, Agriculture, Forestry and Fisheries and the National Veterinary Research and Quarantine Service have mobilized their own methods, but they have yet to find a clear treatment.

The content of advertisements related to ginseng, tissue culture ginseng, red ginseng and other ginsengs is based on the degree of inclusion of ginsenosides and scafponins. This proves that the role of saponin in ginseng and the like is great, and in the process of discovering this, the applicant has confirmed that the superior mountain ginseng has more saponin content and types in Korea.

In other words, the better mountain ginseng is “sweeter and stronger in scent,” which is the word that has been handed down in the oral and ancient books, and the fact that wild ginseng is higher in sugar than any other fruits such as watermelon, strawberry, tangerine, and saponin is higher. The fact that the type or content is large, which revealed that the saponin and sugar content is proportional to the world for the first time, it was intended to apply to the present invention.

The inventors of the present invention, the higher the sugar content of the goat ginseng contains a large amount of saponin, and the saponins have a high antimicrobial and antiviral effect, and tried to apply it to the treatment and prevention of bees infected with cystitis rot disease.

Accordingly, an object of the present invention is to provide a method for the treatment and prevention of viral worms.

It is another object of the present invention to provide an agent for the treatment and prevention of viral worms.

As an example for achieving the above object, the method for treating and preventing viral nematode buds rot of the present invention is mixed with goat ginseng and water in a weight ratio of 1: 1 to 2 to obtain a goat goat extract in a vacuum condition at low temperature, and then concentrated goat goat concentrate Preparing a step, preparing a liquid goat ginseng adjusted to have a sugar content of at least 45BRIX by mixing the goat goat concentrate with water, and diluting the liquid goat ginseng is fed to bees infected or not infected with viral worms Characterized in that it comprises a step.

In particular, the salary for the bees infected with the worm beetle rot disease was diluted in a ratio of 1 g of goat's ginseng and 250 to 300 ml of water, and prepared liquid goat ginseng adjusted to sugar with sugar and then fed at a rate of 400 to 600 ml of liquid goat ginseng per beehive However, the treatment of 20 times a day once a treatment to treat, the beetle not infected with the worm beetle is a healthy goat ginseng diluted by 1.5 g of goat ginseng concentrate and 250 to 300 ml of water and adjusted to sugar with sugar After the production of a bee hive per one liquid goat ginseng 400 to 600ml ratio, it is preferable to prevent it by feeding once every two days.

As another example for achieving the above object, the present invention, goats ginseng and water 1: 1 to 2 by weight ratio by mixing the goats ginseng extract obtained under a low-temperature vacuum condition and then concentrated 1.0 to 1.5 g and water 250 obtained It is characterized by a cystic rodents rot disease treatment and prevention agent comprising a liquid goat ginseng adjusted to a sugar content of at least 45BRIX by mixing at a ratio of 300 to 300ml.

Hereinafter, the present invention will be described in detail with a focus on treatment and prevention methods.

First, a step of obtaining a goat ginseng concentrate.

In the present invention, the goat ginseng is mixed with a small amount of water to obtain a concentrate by vacuum extraction at low temperature. When the concentrate is obtained in this way, it is possible to obtain a goat goat concentrate that reflects the pharmacological effects of goat goat. Ginseng, fresh ginseng, white ginseng, red ginseng can be used instead of goat ginseng, and it is possible to obtain a concentrate with sufficient pharmacological effects such as ginseng even if it is not possible to obtain a concentrate with sufficient saponin content as that of goat ginseng. . In addition, when using the wild ginseng culture root will be able to obtain a similar effect to the wild ginseng, it would be possible to apply a wild ginseng culture root instead of a goat.

In addition, according to the present invention, depending on the harvesting time of goats may be different parts used to obtain a goat goat concentrate, in the case of goats harvested in early May to the end of August can use both roots and shoots and leaves of goats Goat ginseng, which is harvested from early September to late April, should be rooted.

It is preferable that the cultivation period is more than 7 years, and if necessary, the cultivation period is less than this, but it is preferable to use 7 years old rooted for 7 years.

In order to obtain a concentrate of goat's ginseng, goats and water are mixed in a ratio of 1: 1 to 2, preferably 1: 1. In order to maximize the use of saponin contained in a large amount of goat ginseng in the present invention, a goat goat extract is concentrated in a short time under low temperature vacuum conditions.

The extraction of the goat is made in a vacuum, it is preferable that the goat is made at a low temperature in the range of 50 to 90 ℃ in terms of protection of the active ingredient of goat. Goat ginseng extract extracted under the vacuum condition of the low temperature is concentrated under vacuum conditions to obtain a goat goat concentrate. The extraction and concentration of the goat goat is made in a short period of time within 30 minutes to 2 hours.

Next, a liquid goat ginseng is prepared.

In this step, the concentrated goat goat extract is prepared in liquid form to feed the bees to the bees. For this purpose, the goat goat concentrate is mixed with water. At this time, the goat ginseng concentrate is mixed with water to adjust the sugar content to at least 45BRIX. If the sugar content is too low it may contain additional sugar. Goat ginseng is higher in sugar than ordinary ginseng, and thus, the above sweetness is easily achieved. In addition, if the sugar is less than the above range, the bees will not easily eat, it is preferable to adjust the sugar to the above range.

In the present invention, the wild ginseng discarded the preconceived notation that saponin will be used, and by the phrase "the better goat is stronger and sweeter", it was confirmed that the sugar content of goat ginseng was proportional to the saponin content. It is desirable to adjust the above range because it may not be fed to the food.

Next, the liquid goat ginseng is fed to the bees.

The liquid goat ginseng is diluted to feed to bees infected with or not infected with virus worms. The feed for bees infected with worms is 1% goat ginseng concentrate in a ratio of 250 to 300 ml of water, preferably 290 ml of water. After preparing a liquid goat ginseng diluted in sugar and adjusted to sugar content, the liquid goat ginseng per beehive 400 to 600ml ratio, preferably in a liquid goat ginseng 500ml per one beehive, but 20 salary twice a day Treat it. After a total of 40 days, honeybees infected with cystic beetle rot return to immunity and complete prevention, and produce honey normally.

On the other hand, as a method for the prevention of viral cystic rodent rot, the feed for honeybees infected with the cystic rodent rot is diluted 1.5 g of goat ginseng concentrate in a ratio of 250 to 300 ml of water, preferably 290 ml of water, and sugar content in sugar. After preparing a liquid goat ginseng adjusted to control the amount of liquid goat ginseng 400 to 600ml per beehive, preferably in a ratio of 500 ml of liquid goat ginseng per beehive, it is preferable to prevent it by feeding once every two days.

Therefore, goat and ginseng are mixed at a weight ratio of 1: 1 to 2 to obtain a goat goat extract under a low temperature vacuum condition, and then concentrated at 1.0-1.5 g of a goat goat concentrate obtained by concentrating with water at a ratio of 250 to 300 ml to have a sugar content of at least 45 BRIX. Controlled liquid goat ginseng is sufficient as a treatment or prevention of cystic rodent rot.

According to the present invention described above, it is possible to treat bees infected with cystic beetle rot disease, and also to anticipate the effect of preventing infection to the necrotic beetle rot disease.

According to the present invention described above it can be expected to provide an effect of providing a therapeutic and preventive worms worms that can be treated or prevented bees infected with worms.

1 is a photograph of a process of injecting a liquid goat ginseng prepared by the present invention into the body as a bee food.

Figure 2 is a photograph of a bee infected with virus worms rot.

3 is a photograph of a bee treated with viral worms.

Hereinafter, the present invention will be described in detail with reference to examples and drawings, but the present invention is not limited by the following examples.

실시예 1. 산양삼 농축물의 제조Example 1 Preparation of Goat Ginseng Concentrate

From the beginning of May to the end of August, after cleaning the whole root including sprouts and fresh leaves of 7-year-old goats grown in the wild of pesticide-free and wild nature, 100g per 100ml of fresh water is added to the ultra-low vacuum (90 ℃). It was put in a concentrated extractor and operated for 30 minutes to obtain a goat goat concentrate.

실시예 2. 산양삼 농축물의 제조Example 2 Preparation of Goat Ginseng Concentrate

From the beginning of September to the end of April, only the roots of goats with no more than 7 years old shoots and leaves, which are grown in the wild without pesticides and nomenclature, are washed thoroughly. It was put in a concentrated extractor and operated for 30 minutes to obtain a goat goat concentrate.

실시예 3. 바이러스 낭충봉아 부패병에 감염된 꿀벌에 급여Example 3 Feeding to Honey Bees Infected with Virus Cytomegalovirus rot

1 g of goat's ginseng concentrate obtained in Example 1 was mixed with 290 ml of bottled water, and sugar was prepared by mixing until the sugar content of 45BRIX was suitable as a food for bees to prepare a liquid goat.

This was put in the holding container at the bottom of the beehive inhabited bees infected with viral worms decay disease from the first sale, shown in Figure 2 ,. Feed amount was adjusted to 500ml of liquid goat ginseng per beehive, the feeding cycle was adjusted once every two days, the total number of salaries was 20 times 40 days, was confirmed that the treatment was made [Fig. 3].

실시예 4. 바이러스 낭충봉아 부패병에서 회복된 꿀벌에 급여Example 4 Feeding to Honey Bees Recovered from Virus Cytomegalovirus rot

1.5 g of goat's goat concentrate obtained in Example 2 was mixed with 290 ml of mineral water, and sugar was mixed with sugar until the sugar content of 45 BRIX was adjusted to prepare a liquid goat goat.

This was put in a holding container at the bottom of the beehive inhabited by the infected bees recovered from the virus worm rodent rot shown in FIG. Feeding amount was adjusted to 500ml of liquid goat ginseng per beehive. After 15 days of salary, about 1 kg of goat ginseng honey was obtained from a single beehive.

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

Claims

Preparing a goat goat concentrate by mixing goat goat with water at a weight ratio of 1: 1 to 2 to obtain a goat goat extract under a vacuum at low temperature;

Mixing the goat goat concentrate with water to prepare a liquid goat goat adjusted to have a sugar content of at least 45 BRIX, and

Feeding the liquid goat ginseng to bees infected with or not infected with virus worms

Viral cystic rod rot treatment and prevention method comprising a.
The method according to claim 1,

The goat ginseng is a method for the treatment and prevention of virus worms rot disease, characterized in that using the root or roots and shoots and leaves of goats.
The method according to claim 1,

Extract of the goat ginseng is a virus worms rot disease treatment and prevention method, characterized in that made in a low temperature range of 50 to 90 ℃.
The method according to claim 1,

Viral cystitis rot disease, characterized in that to control the sugar content by additionally comprising sugar in the liquid goat ginseng.
The method according to claim 1,

Feeding for the bees infected with the worm beetle rot disease is diluted in the ratio of 1g goat goat concentrate and 250 to 300ml of water, and prepared liquid goat ginseng adjusted to sugar with sugar, and then fed at the rate of 400 to 600ml liquid goat ginseng per beehive, 20 salaries once a day for treatment,

Feeding for honeybees not infected with the worm beetle rot disease was prepared by diluting goat ginseng concentrate 1.5g and water 250 to 300ml ratio and producing a liquid goat ginseng adjusted sugar with sugar at a rate of 400 to 600ml liquid goat ginseng per beehive Viral worms and rot disease treatment and prevention method characterized in that the salary, but prevented by once every two days.
The method according to claim 5,

Feeding for the bee infected with the cystic rodents rot disease was diluted in the ratio of 1 g of goat's ginseng concentrate and 290ml of water, and administered liquid goat ginseng with sugar content adjusted to sugar to be administered to the honeybees infected with cystworm rot,

Feeding for the bees not infected with the cystic rodents rot disease is a cystic rodents rot disease, characterized in that the administration of a liquid goat ginseng dilution of 1.5 g of goats ginseng concentrate and 290 ml of water and adjusted the sugar content with sugar to prevent cystic rodents rot Treatment and prevention methods.
Goat ginseng and water is mixed at a weight ratio of 1: 1 to 2 to obtain a wild ginseng extract under a low temperature vacuum condition, and then concentrated at a rate of at least 45BRIX by mixing 1.0-1.5 g of concentrated goat ginseng concentrate at a ratio of 250-300 ml of water. Cystic beetle rot disease treatment and prevention agent comprising a liquid goat ginseng.