KR20130071028A - Agent for sacbrood virus of bees - Google Patents

Abstract

PURPOSE: A therapeutic agent for treating sacbrood virus of bees is provided to protect honey bees from infection by sacbrood by injecting to a beehive before the bees are infected. CONSTITUTION: A therapeutic agent for treating sacbrood virus of bees contains 0.1-10 wt% of thymol, 0.1-10 wt% of camphorum, 0.1-10 wt% of tea tree oil, 0.1-10 wt% of pinene, 0.1-5 wt% of chlorine dioxide, 0.1-5 wt% of acetic acid, 0.1-5 wt% of a red ginseng extract, and 50-80 wt% of purified water. The therapeutic agent is used by mixing with 1L of sugar liquid. The therapeutic agent is injected to a beehive with a sponge containing a disinfectant for beekeeping. When larvae of honey bees are dead, the therapeutic agent is used after removing eggs and larvae. [Reference numerals] (AA) Average accumulated number of dead larvae; (BB) The number of dead larvae; (CC) A group; (DD) B group; (EE) C group; (FF) D group; (GG) Artificial infection

Description

AGENT FOR SACBROOD VIRUS OF BEES

The present invention relates to an agent for treating beetleworm rot of bees, and more particularly, to a bee virus cure agent for treating bee bugworms because of thymol and stabilized chlorine dioxide having excellent bactericidal effects.

There is much interest in the world about the disease that has occurred in honeybees due to the recent efforts to protect honeybees and products related to honeybee products. Bee parasitic viruses are found throughout the world.

The most common and common among such viruses are Deformed Wing Virus (DWV), Acute Bee Paralysis Virus (ABPV), Chronic Bee Paralysis Virus (CBPV), and Black Queen Cell Virus. (Black Queen Cell Virus; BQCV), Kashmir Bee Virus (KBV), and Sacbrood Bee Virus (SBV). Most of these viruses infect bees without any syndrome.

Currently, the beekeeping scale of Korea is about 2 million counties in 40,000 households. Most beekeeping farmers are suffering from bee mite, gypsum disease, old horse disease, buzzer disease, and wormworm rot disease.

In particular, native farms are suffering from worms, which are viral diseases, but there is no officially approved treatment, and the use of unvalidated drugs should be improved quickly.

In 2009, Chinese sacbrood virus (CSBV) was identified in domestic bees (American bee: Apis cerana). The virus is indigenous to the Asian continent and has been known to evolve with Asian bees for many years. Since its discovery in Thailand in 1981, it has been found in nearly all Asian countries with distributions of Indian, Pakistan, Nepal and Asian bees. It is believed that larvae are infected through secretions from the glands of the nurse bee, which is responsible for all child-rearing activities such as feeding milk (royal jelly) or feeding (honey and pollen). In Korea, it started to occur in spring 2009 and spread throughout the country. As of July 2011, about 90% of native bees are reported to have died.

In the Tobong area, where the epidemic occurred, all the rebels became infected within about a month, and died in about one month thereafter. If the virus does not reach the indigenous area, the rebels can survive, and if the virus develops, it is currently impossible to prevent or treat it. In order to prevent spread, it is known that all native bees within a radius of six kilometers must be killed.

According to the Korea Tobong (National Bee) Association, more than 55,000 native bees have been infected with worms and worms from 2,500 native bee farms in Chungcheong, Jeolla and Gangwon.

The National Veterinary Research and Quarantine Service also surveyed three farms in Namwon, Sunchang, and Imsil, Jeonbuk, and found that more than 70% of Bonggun were infected with this disease. The Farmers Newspaper reported that 39.9% (as of the end of July 2011) of native bee farms in Gangwon, Chungbuk, Jeonnam, Jeonbuk, and Gyeongnam were affected.

According to the Ministry of Food, Agriculture, Forestry and Fisheries, native bees died or became infected in 16,6782 groups, or 49.9% of the 418,000 groups this year.

There are approximately 10,000 bees living in one beehive (referred to as Bonggun). Nangchungbonghyeong disease, which first developed in Gangwon-do in May last year, spread to Chungnam, Chungbuk and Gyeongnam to Jeonnam and Jeonbuk, and it is estimated that half of native bee has already been damaged in Gangwon-do. Occurrence of worms can lead to a sharp drop in production, leading to a sharp rise in native honey prices, as well as to the fact that the bees have died in nature, making natural fertilization difficult, as well as secondary damage to fruit trees and flower farmers.

Prophylactic methods and treatments that are not accurately validated are used, and there are no drugs, prophylaxis or treatments derived from accredited clinical trials as of July 2011.

Study on Sacbrood Virus and Major Royal Jelly Protein family from Honeybee Apis mellifera and on AHLase from Bacillus thuringiensis: Part 1.Study on Honeybee Virus and Honeybee Products: Part 2. Study on quorum quenching from Bacillus thuringiensis, Department of Biology, Kyonggi University (2010)

The present invention has been made to solve the problems described above, the object of the present invention is to prevent the spread of worms rot decay disease, a kind of viral disease that is a problem occurring in Tobong farms and to protect the native bees It is intended to provide a cure for bony rot.

In order to achieve the above object, cystic rodent rot treatment agent of the present invention, thymol 0.1-10% by weight, camphor 0.1-10% by weight, tea tree oil 0.1-10% by weight, Pinene 0.1-10% by weight, chlorine dioxide water (Chlorine dioxide) 0.1-5% by weight, 0.1-5% by weight acetic acid and 0.1-5% by weight red ginseng extract and 50-80% by weight purified water.

In the nematode rodent rot treatment agent according to the present invention, the specification drug solution made by mixing 0.005 ~ 0.02L worm rodent rot disease treatment agent to 1L of 50% by weight sugar solution is used.

In the nematode rodent rot treatment agent according to the present invention, the rodent rodent rot treatment agent is injected from the beehive installed at the entrance of the bee with the sponge which absorbed the bee disinfection agent.

In the nematode rodent rot treatment agent according to the present invention, when bee larvae die in the beehive beehives, it removes the bee spawning and granulation from the beehives, and puts a scattering plate free of contamination to stabilize the bee group and perform disinfection. .

In the nematode rodent rot treatment agent according to the present invention, the beetle rodent rot treatment agent is administered to bees once or twice a day.

If the therapeutic agent for cystic beetle rot disease according to the present invention is put into a beehive before infection and continuously preventive treatment for honey bees, it is possible to protect the honey bee without being infected with cystic beetle rot disease.

Survival rate of 22% or more can be confirmed when the composition for cystic beetle rot disease according to the present invention is put into the pre-infection Bong-gun. Increased immunity can prevent bees from dying and be a means of restoring the rebel forces.

Figure 1 is a graph showing the experimental results of the beetle insect rot virus of the bee according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, it should be understood that the following embodiments are provided so that those skilled in the art will be able to fully understand the present invention, and that various modifications may be made without departing from the scope of the present invention. It is not. Wherein like reference numerals refer to like elements throughout.

The therapeutic agent for cystic beetle rot disease according to the present invention, 0.1-10% by weight of thymol, 0.1-10% by weight of camphor, 0.1-10% by weight of tea tree oil, 0.1-10% by weight of pinene, Chlorine dioxide 0.1-5% by weight, acetic acid (0.1-5% by weight) and red ginseng extract 0.1-5% by weight and purified water 50-80% by weight.

Timol is a natural emulsion found in some plants, such as thyme. It is known that these thymols are effective in controlling bee mite parasites. However, the vaporization of thymol is sensitive to changes in temperature, so when the weather is cold, the mite control efficiency is lowered, while the degree of vaporization in the hot summer is increased, and the bee is toxic to bees. In order to make up for the shortcomings, the gel was developed in order to cope with the vaporization of thymol, and a product using the microcapsule technique has recently been developed.

Thus, when the thymol, a tick control agent having a liquid form bee mite control effect of the thymol component of the essential oil, is administered in a spray method, a drip method or a shedding method, the killing effect on the bee mite is known to be excellent.

Camphor is an aromatic organic compound that is permeable and has a mildew odor. The molecular formula is C ₁₀ H ₁₆ O and has been used for centuries as a component of fragrances and medicines. In modern times, it is used as a plasticizer and insect repellent (particularly as an insect repellent against moths) used to make cellulose nitrate. It is mainly produced from camphor trees in China, Taiwan and Japan. The camphor is separated by condensing the gas produced by passing steam through the powdered wood. Camphor is crystallized from the oil component of the distillate and is purified by compression and sublimation.

Since the early 1930's, camphors have been made in alpha-α-Pyenne through several processes. Camphor is one of the organic compounds belonging to the terpene type ketone. In 19th-century organic chemistry, studying the structure of camphor and its response was an important task. Pure compound is white and has a melting point of 178 ~ 179 ℃ as a smooth solid.

Tea tree oil shows broad spectrum antimicrobial activity. The ability of tea tree oil to disrupt the permeation walls of cell membrane structures, and thus the loss of its ability to control chemical penetration, is due to the lethal action of tea tree oil. In addition, a placebo controlled previous study with tea tree oil in the treatment of herpes rashes found that tea tree oil had a similar effect to 5% asaclovir.

Tea tree oil has been reported to be effective as a local disinfectant that kills the bacteria that cause acne.

Pinene is a hydrocarbon belonging to the monoterpenes, and has two ring structures and one double bond in the molecule. There are three positional isomers and respective optical isomers according to the difference of the double bonds.

Formula C ₁₀ H ₁₆ has a molecular weight of 136.24. Insoluble in water, but insoluble in organic solvents such as ethanol, ether and benzene. α-pinene is a main component of terebin oil in both d-type and l-type, and is widely distributed in other essential oils, including conifer oil. Melting point -55 ℃, boiling point 156 ℃, specific gravity 0.87. It is a colorless liquid with a fresh scent.

Used as a solvent for paints, synthetic raw materials for camphor. β-pinene coexists with α-pinene, but in small amounts. It is a colorless liquid, melting point -50 ℃, boiling point 165 ℃, specific gravity 0.87. δ-pinene does not exist in nature, but is synthesized from tosylate of d-isoberbanol and d-pinocamperol, respectively. The d-cis isomer is a colorless liquid with a boiling point of 159-161 ° C. The l-trans isomer is a colorless liquid with a boiling point of 157 to 158 ° C.

Chlorine dioxide (ClO ₂ ) is a non-chlorine disinfectant that is a water-soluble oxidant in which pure chlorine dioxide gas has the strongest sterilizing power and deodorizing bleaching power following ozone. It is harmless to human body, and it is an environmentally friendly substance that does not produce carcinogens such as trihalomethane and is easily decomposed by light.

Chlorine dioxide is a low concentration of a variety of bacteria (fungal) bacteria such as avian influenza, swine flu (H1N1), superbacteria, acne, fungi, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella, Candida, etc. It does not produce harmful by-products such as trihalomethane (THM) and haloisetonitrile (HAN).

Acetic acid is a colorless liquid with an irritating odor and acidity. Carbon, oxygen, hydrogen compounds, acidic weak monobasic acid. It is a raw material such as glacial acetic acid fiber, glacial acetic acid vinyl, anhydrous glacial acetic acid, acetone, etc., and has a characteristic of being used in fiber processing and the like.

Red ginseng extract refers to ginseng extract. Ginseng is known to have an immunological function. Especially, red ginseng extract activates and increases the function of natural killer cells in rats and increases cytotoxic immunity and antibody-dependent cytotoxicity. Other studies have also suggested that ginseng acts as an anticancer by activating the host's immune system.

Distilled water is used as a solvent that can be mixed therein.

Honey bee worms are given once or twice daily.

Cystic beetle rot disease treatment agent is a specification drug made by mixing 0.005 to 0.02 liters of cystic beetle rot disease treatment agent in 1 liter of 50% by weight sugar solution.

The cystic rod decay treatment agent is preferably injected from a beehive installed at the entrance of the bee together with the sponge absorbing the beekeeping disinfectant. In addition, when bee larvae die in the beehive beehives, it is preferable to remove the bees spawning and granulation from the beehives, and to put the scattering plate free of contamination to stabilize the bee group and perform disinfection.

In the following, embodiments of the bee worm worm rot disease treatment agent according to the present invention will be described in more detail, the present invention is not limited to the following examples.

≪ Example 1 >

The test drug was mixed with 150 ml of a bee worm bug rot decay disease drug in 50 weight% 18 liters to make a specification drug. The test drug for the wormworm worms, 0.1-10% by weight of thymol, 0.1-10% by weight of camphor, 0.1-10% by weight of tea tree oil, 0.1-10% by weight of pinene, 0.1-5% by weight of chlorine dioxide %, Acetic acid (0.1-5% by weight) and red ginseng extract 0.1-5% by weight and purified water 50-80% by weight.

The test drug thus prepared was administered orally in the manner of refraining about once every 10 days or every other day.

Hereinafter, the method of dividing the test drug according to the item to be tested will be described in detail.

Group A is a test group of prophylactic effects on the treatment of beeworm rot decay. Analysis of honey bee swarm prior to administration of bee nematode rot rot treatment should be negative. In order to confirm the preventive effect of the beetle beetle rot virus, the bee was administered once a day before artificial infection for 10 days. The next day after the completion of the administration artificial infection of the cystic rodent rot virus was confirmed, the virus prevention effect verification and safety was confirmed.

Group B is the result of the bee group's initial treatment effect test. As in Group A, the results of an analysis of honey bee swarm prior to administration of a bee worm decay disease treatment should be negative.

In order to confirm the initial treatment effect of bee worm rodentine rot virus, it was administered once a day for 10 days based on the 50 ml of the above-mentioned specification chemicals at the same time as the artificial infection at the time of sunset for the virus infection effect and safety. Confirmed.

Group C is a test for the identification of the therapeutic effect of the bee worm bug rot virus. In order to confirm the therapeutic effect, group C was collected from the digestive tract of artificially infected Sungbong rods by PCR (polymerase chain reaction; PCR) technique. Confirmation of infection and positivity of decay disease was confirmed, and the therapeutic effect and safety of the virus were checked by administering a cure for beetle worm decay disease, which is a therapeutic drug.

< Experimental Example  1> open air effect test

One -1 virus eradication test inside the body of a bee

In order to perform a test for the parasitic virus in the body of the bee to feed the bees to the specification drug made by mixing the beetle bug rot decay virus treatment of the bee according to the present invention to determine whether the bee virus is treated in the gut .

1-2 virus-proof test outside the body of a bee

In order to check whether there is a therapeutic effect against the bee worm bug rot virus outside the body of the bee, the bee disinfectant for external beehives was first disinfected. The above-mentioned beekeeper disinfectant is diluted 50 times to disinfect internal and external beehives including bees, and disinfects beekeepers using spray or precipitation method and absorbs beekeeper disinfectant for the purpose of prevention and disinfection until the treatment is completed. A sponge was installed at the entrance of the bee, allowing the bees to step on it.

Beekeeping disinfectants include organic acid, sodium bicarbonate, including any one selected from the group consisting of potassium perpersium monopersulfate, sulfamic acid, adipic acid, and combinations thereof. A foamed disinfectant comprising a carbonate, a surfactant, and a binder, wherein the foamed disinfectant comprises 45 to 50 parts by weight of potassium peroxide, 10 to 25 parts by weight of sulfamic acid, and adipic acid based on 100 parts by weight of the total of the foamed disinfectant. 1.0 to 15.0 parts by weight, 7.5 to 15.0 parts by weight of sodium bicarbonate, 0.2 to 2.0 parts by weight of a surfactant including α-olefin sulfonate as a surfactant, polyvinylpyrrolidone and hydroxyl propylmethylcellulose as a binder 1.0 to 5.0 parts by weight of the binder, 0.5 to 5.0 parts by weight of an oxidizing aid including sodium chloride, sodium hexameta Stabilizer 3.0 to 7.5 parts by weight comprising a switch sulfate (sodium hexametaphosphate), and a foam-type disinfectant comprises dye and a flavoring 0.01 to 1.0 parts by weight.

These foamed disinfectants have excellent disinfection power and can be easily obtained by quantifying disinfectant as needed by simply putting in water, and because they are foamed tablets, they can be completely dissolved in a short time without dissolving device, thereby reducing work time. It can be stored in the form of tablets, which reduces the cost of storage, handling and transportation, and in particular, the concentration or amount can be adjusted as needed, so there is no overdose or waste, and the disinfectant component is blown away as when using granules or powder formulations. It is safe to handle because there is no workable factor, and it is easy to calculate the standard volume when the dilution concentration needs to be simplified due to the workplace conditions.

In order to establish a general environment for this external parasitic virus eradication test, a test rod was selected as a place where no other rods were located within a radius of 4 km starting from an outdoor clinical test rod. The measures described above were intended to ensure that the spread of dobong and other diseases would not interfere with the test.

At this time, the bee breeding method was carried out in an improved hive of the langstrope type, which had a length × width × high = 500 mm × 550 mm × 430 mm (outer diameter).

In selecting the test group, the group was a healthy group with similar taxes and no disease infection.

When the field test site was promoted, sterilization was performed with a beekeeper disinfectant and placed in advance 10 days before the experiment 2 to stabilize the bees to be used in the experiment 2.

In order to ensure that the test results for each test example do not influence each other, the beehives are spaced at least 4 m apart to give a unique number and to check the number of insects and sand sticks.

Table 1 is a table comparing the classification and the method according to the conditions of the experiment according to the present invention.

division Prevention effect test group (infection after drug administration) Initial infection test group (dose on the day of infection) Treatment effect test after confirmation of infection No-Drug Control Classification Group A Group B Group C Group D Breeding way Improvement Method Improvement Method Improvement Method Improvement Method Bonggun 5 5 5 5 number A1-A5 C1-C5 C1-C5 D1-D5

For the automatic feeding of the liquid feed, a liquid feed automatic specification feeding system was installed 10 days before the experiment 2 was conducted. The feed used at this time was the specification solution and potted rice cake.

Table 2 is a table showing the composition of the specification solution and potted rice cake.

division Manufacturing method Specification Chemical Water: Sugar = 50:50 (50% sugar) Potted rice cake Natural pot: Sugar: Water = 5: 5: 1.5 mixed rice cake form

The above specifications chemical solution was administered once a day or every other day at a rate of 50 ml per sheet at the time of sunset to prevent the dobong to be a smooth outdoor experiment.

Potted rice cake began the experiment after supplying more than 1kg and used a method of adding if insufficient.

< Experimental Example  2> Nymphworm  Corruption virus culture experiment

Larvae infected with the cystic rod rot virus were collected and stored at -80 ° C in a sealed state and used within 10 days. To determine whether the infected larvae were infected with the worm-worm virus, the estimated larvae were first frozen with liquid nitrogen, crushed and placed in a 1.5 ml eppendrof tube. RNA (ribonucleic acid) was isolated and finally eluted in 50 μl RNase-free water. Total RNA was synthesized cDNA by reverse transcriptase reaction PCR with oligo -dT primer and cDNA synthesis kit (promega). PCR was performed using SBV-F (5`-gctgaggtaggatctttgcgt-3`) and SBV-R (5`-tcatcatcttc accatccga-3`) primers, which are gene-specific primers of the beetle rot virus, using the synthesized cDNA as a template. The infection was determined by comparing the sequence of cDNA amplified by the nucleotide sequence registered in the gene bank. 2 g of the confirmed larvae were placed in a 6 ml sterile PBS (phosphate buffered solution) (137 mM NaCl, 2.7 mM KCl, 10 mM Na ₂ HPO ₄ , pH 7.4), crushed, and centrifuged at 12,000 rpm for 10 minutes at 4 ° C to separate the supernatant. Virus was recovered.

2 -2 Virus Culture Using Bee Larvae

Bee larvae were transferred to cell culture plates and improved artificial specification solution (6% D-glucose, 6% D-fructose, 1% enzyme extract at 37 ° C, 90% humidity). extracts, 50% royal jelly, and the recovered virus were incubated for 2 days, and 0.2 g of the cultured larvae were 6 ml sterile PBS (phosphate buffered solution) (137 mM NaCl, 2.7 mM KCl, 10 mM Na ₂ HPO _4). , pH 7.4), pulverized, and centrifuged at 12,000 rpm for 4 minutes to separate the supernatant and recover the virus. The recovered virus supernatant was measured for virus concentration (copies / μl) using a real time PCR method and diluted to 1 × 10 ⁵ (copies / ml) for the experiment.

< Experimental Example  3> Artificial infection method, infection status check method and treatment method

3-1 Artificial Infection Methods

80 ml of 50% sugar syrup was mixed with 10 ⁵ copies / ml of nematode rot disease virus and 2 ml aliquots per group were infected by adult feeding or spraying.

3-2 How to check for infection

One week after the artificial infection, the digestive tract of the artificially infected Seongbong was harvested and confirmed by infection with cystic beetle rot.

< Experimental Example  4> Evaluation method and evaluation standard of outdoor experiment safety

4-1 Evaluation Method

Visually confirm the degree of digestion of the supplied amount of the specification drug solution made with the test drug once daily, based on 50 ml per consumption. (Observed while feeding for 10 days.)

After dosing, the bee's disturbance and the number of sand bars within 2m of the experimental Bonggun during the experiment shall be checked visually.

Check for doping due to the experimental drug. Check if there are any side effects such as stopping spawning due to the experimental drug.

4-2 Evaluation Criteria

The total amount of the specifications solution prepared as experimental drug should be digested once a day based on 50 ml per sheet.

There should be no disturbance of bees after administration of the test agent, and there should be no bee due to the test agent within 2 meters of the slope of the test group during the test period. There should be no dosing caused by the test agent. There should be no side effects such as interruption of spawning due to the experimental drug.

<Experimental Results>

Group A prevention effect test result

Although the degree of mite infection was similar, it was found to be positive as in other experimental groups. The total number of worms was 1132, and compared with the worms of the experimental group D (1462). The survival rate increased by about 22%. RT-PCR also confirmed that the forces of the virus of cystic rodent rot were weakened. In the case of bee worm bug rot decay treatment according to the present invention was considered to be a way to prevent the death of the bee and to restore the forces of the bee bong by increasing the immunity of the bee when treated in a preventive manner before the virus infection.

Experimental results of early treatment of group B infection

The degree of infection of the mites was similar, but the infection of the worms was confirmed according to the test method. As a result of the confirmation of infection, it was positive like other experimental groups, and 1343 worms totaled. The survival rate was increased by about 8% compared to the non-treated group D.

Group C After infection  Treatment effect confirmation experiment

The degree of mite infection was similar, but infection of cystic rod rot was confirmed according to the test method.

As a result of infection, it was found to be positive like other experimental groups. The total number of 1407 insects was similar, and the survival rate was similar when compared to the insects of the experimental group D (1462), which showed similar trends in PCR results. Showed

Table 3 is a table showing the results of insect killing confirmation.

Military number Infection 0day 10day 10day 17day 24day 31day Group A A-1 - - sign

ball

feeling

salt +++ ++ + A-2 - - +++ ++ + A-3 - - +++ ++ ++ A-4 - - +++ ++ + A-5 - - +++ ++ ++ Group B B-1 - - +++ +++ +++ B-2 - - +++ +++ +++ B-3 - - +++ +++ +++ B-4 - - +++ +++ +++ B-5 - - +++ +++ +++ Group C C-1 - - +++ +++ +++ C-2 - - +++ +++ +++ C-3 - - +++ +++ +++ C-4 - - +++ +++ +++ C-5 - - +++ +++ +++ Group D D-1 - - +++ +++ +++ D-2 - - +++ +++ +++ D-3 - - +++ +++ +++ D-4 - - +++ +++ +++ D-5 - - +++ +++ +++

Figure 5 is a table showing the experimental results of the beetle insects rot virus of the bee according to the present invention.

Referring to Figure 5, it can be seen that the number of larvae significantly decreased compared to the other experimental group only in the case of group A.

< Experimental Example 5 > Outdoor Experiment Safety Assessment Results

The indicators used as the safety evaluation criteria were judged based on the degree of consumption of the specification drug, the number of the four-slopes within 2m of the experimental Bong-gun, the presence or absence of the rods and the stopping of spawning.

5-1 Safety

In the case of the bee worm bug rot disease treatment according to the present invention, the bees did not cause a disturbance, and no sapons were identified.

Table 4 is a table showing the safety results for the bee according to the present invention for the treatment of worms bugs rot.

division 1st test Second test Remarks Total amount Average Total amount Average Dosing group total salary (10 groups) 138 13.8 142 14.2 Non-medicated group natural sapon (Group 5) 66 13.1 62 12.4 Sabongsu compared to natural 0.7 1.8

5-2 side effects

No side effects were observed with the use of a bee worm bug rot decay agent according to the present invention. There was a record that bees did not eat due to the odor of the therapeutic agent when excessively used in excess of the dosage.

As mentioned above, although preferred embodiment of this invention was described in detail, this invention is not limited to the said embodiment, A various deformation | transformation by a person of ordinary skill in the art within the scope of the technical idea of this invention is carried out. This is possible.

Claims (6)

0.1-10% by weight of thymol, 0.1-10% by weight of camphor, 0.1-10% by weight of tea tree oil, 0.1-10% by weight of pinene, 0.1-5% by weight of chlorine dioxide, 0.1% of acetic acid -5% by weight, red ginseng extract 0.1-5% by weight and purified water 50-80% by weight of the bee worms rot rot disease treatment. The method of claim 1,
The cystic rod rot treatment agent is a nymph rot rot disease treatment agent characterized in that using the specification drug made by mixing the cystic rod rot treatment agent 0.005 ~ 0.02L to 1L of 50% by weight sugar solution. The method according to claim 1 or 2,
The beetle bug rot treatment agent is a beetle bug rot disease treatment, characterized in that the bee in the beehive installed in the entrance of the bee together with the sponge absorbing the bee disinfectant The method according to claim 1 or 2,
When the larvae of the bees in the beehive beehive death, bee larvae and rot decay disease, characterized in that to remove the scattering and granulation of the bees from the beehive and to put the contamination-free scattering plate to stabilize the bonggun and disinfect. The method according to claim 1 or 2,
The beetle bug decay treatment agent of bees, characterized in that administered to bees once or twice a day beetle bug rot disease. The method of claim 3,
The bee beekeeping disinfectant includes an organic acid, sodium hydrogencarbonate, including any one selected from the group consisting of potassium perpersium monopersulfate, sulfamic acid, adipic acid, and combinations thereof. It is a foamed disinfectant comprising a carbonate, a surfactant, and a binder, wherein the foamed disinfectant is 45 to 50 parts by weight of the potassium peroxide sulfate, 10 to 25 with respect to 100 parts by weight of the foamed disinfectant Parts by weight, 1.0 to 15.0 parts by weight of the adipic acid, 7.5 to 15.0 parts by weight of the sodium bicarbonate, 0.2 to 2.0 parts by weight of a surfactant containing α-olefin sulfonate as the surfactant, polyvinylpyrrolidone as the binder And 1.0 to 5.0 parts by weight of a binder containing hydroxyl propylmethylcellulose, including sodium chloride. Beetle insects, characterized in that the foaming disinfectant comprising 0.5 to 5.0 parts by weight of the oxidizing aid, 3.0 to 7.5 parts by weight of a stabilizer containing sodium hexametaphosphate, and 0.01 to 1.0 parts by weight of pigments and fragrances. Treatment of bud rot.

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