WO2012008722A2 - 조직 증강용 충전 조성물 - Google Patents
조직 증강용 충전 조성물 Download PDFInfo
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- WO2012008722A2 WO2012008722A2 PCT/KR2011/005072 KR2011005072W WO2012008722A2 WO 2012008722 A2 WO2012008722 A2 WO 2012008722A2 KR 2011005072 W KR2011005072 W KR 2011005072W WO 2012008722 A2 WO2012008722 A2 WO 2012008722A2
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- hyaluronic acid
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- alkylenediamine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/16—Amides, e.g. hydroxamic acids
- A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
- A61K31/167—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the nitrogen of a carboxamide group directly attached to the aromatic ring, e.g. lidocaine, paracetamol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/402—Anaestetics, analgesics, e.g. lidocaine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
Definitions
- the present invention relates to biocompatible filling compositions useful for tissue augmentation.
- Tissue enhancers such as soft tissue enhancers
- soft tissue enhancers have been used for medical and cosmetic purposes. Such augmentation may be done surgically through plastic surgery, or in a non-surgical way to increase the volume of soft tissue by administering a biocompatible material.
- it can be used medically for facial atrophy in HIV-positive patients. Facial atrophy is very thin, referring to the loss of subcutaneous fat in the temples and cheeks.
- it has been used to cosmetically enlarge the volume of cheeks, lips, chest and hips, and reduce fine lines and deep wrinkles on the skin.
- Hyaluronic acid is a biopolymer material in which repeating units composed of N-acetyl-D-glucosamine and D-glucuronic acid are linearly connected. Since it has an ophthalmic surgical aids, joint function improvers, drug delivery materials, eye drops, anti-wrinkle agents and the like are widely used in medical and medical applications or cosmetics. However, hyaluronic acid itself has a short half-life of only a few hours in the body, so the application is limited, and studies have been conducted to increase the half-life (persistence in the body) through crosslinking.
- 5,827,937 discloses a method for preparing a hyaluronic derivative crosslinked product using a multifunctional epoxy compound as a crosslinking agent, and 1,4-butanediol diglycidyl ether as a multifunctional epoxy compound.
- BDDE the hyaluronic acid cross-linked water a hydrogel form of Restylane ® produced by using as crosslinking agents are commercially available throughout the world as a filler for tissue enhancement, with the approval of the United States FDA.
- the recognition site of hyaluronic acid degrading enzyme is a carboxyl group of hyaluronic acid, and thus adipic dihydrazide (ADH) which binds to the carboxyl group of hyaluronic acid is used.
- ADH adipic dihydrazide
- hyaluronic acid-ADH crosslinked hydrogels showed significantly higher biopersistance compared to commercially available hyaluronic crosslinked hydrogels, but due to their low viscoelasticity, they are not brittle, do not swell well, and are not evenly distributed in tissues. A problem was found.
- the hyaluronic acid crosslinked product is manufactured using a chemical that can be recognized as a foreign substance in the body as a crosslinking agent, when it is subsequently administered to a living body and decomposed, there may be a problem such as an immune reaction due to the remaining crosslinking agent.
- the amount of crosslinking agents or additives that can act as foreign substances during the manufacturing process should be minimized.
- physicochemical properties such as viscoelasticity required for tissue correction should be maintained even after sterilization.
- the present invention is intended to solve the problems of the prior art as described above, so that it can be used as an excellent tissue correction filler, excellent in vivo sustainability and biocompatibility, good physical properties such as viscoelasticity required for tissue correction even after sterilization It is an object of the present invention to provide a composition comprising a crosslinked hyaluronic acid hydrogel, which can be easily filled with an injection, and injected into biological tissues, and a method of preparing the same.
- the present invention provides a filling composition for tissue enhancement comprising a hydrogel of the hyaluronic acid and alkylenediamine crosslinked formula of the following formula 1;
- HA represents hyaluronic acid or a salt thereof except one carboxyl group
- R 1 is a C 3 -C 10 alkylene group unsubstituted or substituted with hydroxy, C 1 -C 6 alkyl, or C 1 -C 6 alkoxy
- m and n are integers of 10,000 to 4,000,000, preferably 20,000 to 3,000,000).
- the hyaluronic acid and alkylenediamine crosslinked hydrogel is included in 1 to 3 (w / w)% based on the total weight of the composition.
- the composition further comprises unmodified (ie, uncrosslinked) hyaluronic acid, thereby reducing the extrusion force of the composition, so that it can be easily injected into tissue by filling into a syringe.
- unmodified (ie, uncrosslinked) hyaluronic acid thereby reducing the extrusion force of the composition, so that it can be easily injected into tissue by filling into a syringe.
- the unmodified hyaluronic acid is included at 0.05 to 1 (w / w)% based on the total weight of the composition.
- the composition further comprises lidocaine in the hydrogel and unmodified hyaluronic acid of the hyaluronic acid and the alkylenediamine crosslinked product, thereby preventing the elasticity decrease after sterilization of the composition and exhibiting local anesthetic effects.
- the lidocaine is included in 0.1 to 0.4 (w / w)% based on the total weight of the composition.
- composition consists of pharmaceutically suitable carriers such as water, saline, and the like.
- the molecular weight of hyaluronic acid is preferably 20,000 Daltons to 4,000,000 Daltons.
- the alkylenediamine is preferably hexamethylenediamine.
- the hyaluronic acid and the alkylenediamine crosslinked hydrogel are preferably selected from the group consisting of hydroxy, C 1 -C 6 alkyl, hyaluronic acid at pH 5.5 to 6.5 in the presence of a carboxyl activator and a peptide binding promoter. Or reacted with a C 3 -C 10 alkylene diamine compound substituted or unsubstituted with C 1 -C 6 alkoxy.
- the crosslinking rate of the hyaluronic acid and the alkylenediamine crosslinked hydrogel is preferably 5 to 35% (TNBS assay).
- tissue enhancing filler means an increase in tissue volume (eg tissue enlargement) and / or an improvement in tissue function.
- tissue enhancing filler of the present invention may be used to indicate the increase in tissue volume or improvement of tissue function of soft tissues medically or cosmetically, in particular to remove or improve wrinkles of the skin; It can be used to enlarge the volume of body parts such as cheeks, lips, chest and hips.
- hyaluronic acid is used herein to mean both hyaluronic acid itself as well as salts and derivatives thereof.
- aqueous solution of hyaluronic acid as used below is a concept including all of aqueous solutions of hyaluronic acid, aqueous solutions of hyaluronic acid salts, and mixtures thereof.
- the present invention also provides a method for preparing the composition of the present invention, which
- the method of the present invention preferably dissolves the carboxyl group activator and the peptide bond promoter in water and adds the hyaluronic acid and the alkylene diamine compound mixture to the mixture of hyaluronic acid and the alkylene diamine compound within 30 minutes.
- Water hydrogels can be prepared.
- the hyaluronic acid and the alkylenediamine are sufficiently and completely crosslinked by allowing the reaction system to stand at 30 ° C. to 50 ° C. for at least 9 hours without agitation. Rendiamine crosslinked hydrogels can be prepared.
- the method of the present invention is preferably sterilized by using a small amount of crosslinker (alkylenediamine) and auxiliaries (carboxyl group activator and peptide bond promoter) by preparing a hyaluronic acid and alkylenediamine crosslinked hydrogel at pH 5.5 to 6.5. Subsequently, hyaluronic acid and alkylenediamine crosslinked hydrogel having excellent physical properties such as elastic modulus and high biocompatibility can be prepared.
- crosslinker alkylenediamine
- auxiliaries carboxyl group activator and peptide bond promoter
- the method of the present invention by homogenizing the prepared hydrogel sieve, to obtain a homogenized hydrogel of uniform size and narrow particle distribution, showing a relatively low protrusion pressure even after sterilization easily filled into the syringe tissue Injectable compositions can be prepared.
- the method of the present invention is readily precipitated with an aqueous solution of C 1 -C 6 alcohol and washed with phosphate buffered saline (PBS) or sodium chloride buffer to facilitate alkylenediamine, carboxyl group activator and peptide in a short time. Unreacted materials such as binding promoters can be removed.
- PBS phosphate buffered saline
- sodium chloride buffer to facilitate alkylenediamine, carboxyl group activator and peptide in a short time. Unreacted materials such as binding promoters can be removed.
- hyaluronic acid and alkylenediamine crosslinked hydrogel according to the present invention, a composition comprising the same and a preparation method thereof will be described in detail below.
- Hyaluronic acid and alkylenediamine crosslinked hydrogels are prepared by reacting hyaluronic acid with an alkylene diamine compound in the presence of a carboxyl group activator and a peptide bond promoter.
- the hyaluronic acid (HA) used has a molecular weight of 10,000 Daltons to 4,000,000 Daltons, more preferably 20,000 Daltons to 3,000,000.
- In vivo degradation rate of the crosslinked hydrogel prepared by adjusting the molecular weight or concentration of HA can be controlled.
- the higher the initial concentration of HA the higher the crosslinking rate of the crosslinked hydrogel to be produced, and thus lower degradation rate in vivo.
- HA is used at a concentration of 1 to 3.5 (w / w)%, preferably 3 (w / w)%. If it is less than 1 (w / w)%, it is difficult to obtain a desired degree of biodegradation, and if it exceeds 3.5 (w / w)%, the prepared hydrogel is easily dried and broken.
- the alkylene diamine compound is a C 3 -C 10 alkylene diamine compound unsubstituted or substituted with hydroxy, C 1 -C 6 alkyl, or C 1 -C 6 alkoxy, preferably C 3 -C 7 alkylenediamine More preferably C 4 -C 6 alkylene diamine, most preferably C 6 alkylenediamine (hexamethylenediamine), 3.5 to 80 mole%, preferably 10 to 30 mole of the hyaluronic acid repeat unit %, Most preferably from 10 to 25 mole%.
- 1-alkyl-3- (3-dimethylaminopropyl) such as 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide) ) Carbodiimides; 1-alkyl-3- (3- (such as 1-ethyl-3- (3- (trimethylammonio) propyl) carbodiimide (ETC, 1-ethyl-3- (3- (trimethylammonio) propyl) carbodiimide) Trimethylammonio) propyl) carbodiimides; 1-cycloalkyl-3- (2-morpholinoethyl) such as 1-cyclohexyl-3- (2-morpholinoethyl) carbodiimide (CMC, 1-cyclohexyl-3- (2-morpholinoethyl) carbodiimide) Carbodiimide of water-soluble car
- Examples of the peptide bond promoters include 1-hydroxybenzotriazole (HOBt) and 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HOOBt). , 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine), 1-hydroxy-7-azabenzotriazole (HOAt, 1-hydroxy-7-azabenzotriazole), sulfo-N -Hydroxysulfosuccinimide (Sulfo-NHS, Sulfo-N-hydroxysulfosuccinimide), O- (1H-benzotriazole-1-y) -N, N, N ', N'-tetramethyluronium tetrafluoro Borate (TBTU, O- (1H-benzotriazole-1-yl) -N, N, N ', N'-tetramethyluronium tetrafluoro Borate (TBTU
- FIG. 1 shows a hyaluronic acid-hexamethylenediamine crosslinked product prepared using hexamethylenediamine (HMDA) as the alkylene diamine compound.
- HMDA hexamethylenediamine
- the carboxyl group of hyaluronic acid is an important group involved in the recognition of hyaluronic acid by hyaluronidase, a hyaluronic acid degrading enzyme, and the hyaluronic acid and alkylenediamine crosslinked product of the present invention is used in the living body by using the carboxyl group of hyaluronic acid for crosslinking. Minimization of hyaluronic acid degradation by degrading enzymes at the time of infusion increases bioavailability.
- the (-) charge of the carboxyl group affects physical properties such as swelling properties of the hydrogel, which greatly contributes to tissue enhancement.
- the crosslinking reaction is usually carried out in water.
- the hyaluronic acid, the alkylene diamine compound, the carboxyl group activator and the peptide bond promoter may be dissolved in water and mixed, respectively. Also good.
- the transformation may occur at weak acidity into N -ethyl- N- (3-dimethylaminopropyl) urea (EDU), so that the EDC is dissolved in water and crosslinked with hyaluronic acid and alkylene diamine mixture.
- EDC N -ethyl- N- (3-dimethylaminopropyl) urea
- the time required for the addition to a long time the physical properties of the prepared crosslinked hydrogel may be deteriorated, such as the elastic modulus of the composition is lowered after sterilization.
- the time required from dissolution to input is within 10 to 30 minutes, and most preferably within 10 to 20 minutes.
- reaction temperature is maintained at 30-50 degreeC, Preferably it is 43-45 degreeC.
- the hydrogelation reaction proceeds efficiently at this temperature, and usually hydrogel is produced within 30 minutes. It is also possible to prepare a hydrogel having good viscoelasticity by standing at this temperature for at least 9 hours. At this time, it is preferable to leave it without stirring. As the additional reaction time increases, physical properties such as viscoelasticity are increased, and there is almost no change in physical properties such as viscoelasticity of the prepared hydrogel at 9 hours or more.
- the crosslinking rate of the prepared crosslinked hydrogel varies according to pH at the time of manufacture, and physical properties may be changed.
- the pH can be adjusted by adding NaOH aqueous solution to the reaction solution, preferably 5.5 to 6.5, more preferably 6.0 to 6.3.
- reaction aid e.g., carboxyl group activator such as EDC and peptide bond promoter such as HOBt
- Hyaluronic acid and alkylenediamine crosslinked hydrogels having physical properties and without change in physicochemical properties such as viscoelasticity can be prepared even after sterilization.
- Hydrogels prepared in an acidic environment with a pH of less than 5.5 exhibit a relatively low modulus of elasticity (G ′) after preparation, and may further degrade elasticity during sterilization.
- the prepared hydrogel is passed through a sieve or homogenizer is used to form a uniform particle.
- a sieve or homogenizer is used to form a uniform particle.
- the buffer is preferably a PBS solution or sodium chloride (NaCl) solution, preferably using a sodium chloride (NaCl) solution.
- an aqueous ethanol solution such as an 80% aqueous ethanol solution is added, hydrogel particles are produced by precipitation, in which unreacted substances are easily removed.
- the precipitated hydrogel may be dried and powdered with nitrogen gas, and the powdered hydrogel may be rehydrated with a physiologically suitable aqueous solution.
- PBS or saline is preferable as a physiologically suitable aqueous solution, and may be used by rehydrating a physiologically suitable aqueous solution at a ratio of 20 to 30 (volume ratio) with respect to the hyaluronic acid-alkyldiamine crosslinked hydrogel powder. .
- the hyaluronic acid-alkylenediamine crosslinked hydrogel of the present invention has a crosslinking rate of 5 to 35%, and preferably has a crosslinking rate of 10 to 20%. If the crosslinking rate is less than 5%, the ratio of unreacted COOH groups not involved in crosslinking in hyaluronic acid is increased, so that it is easily decomposed by hyaluronidase, a degrading enzyme, and thus it is difficult to obtain a desired degree of biopersistence. .
- crosslinking rate exceeds 35%, it is difficult to show the degree of swelling and viscoelasticity useful for tissue enhancement, and the biocompatibility is low, such as the possibility of causing an inflammatory reaction due to a high content of residual crosslinking agent after degradation in vivo.
- the crosslinking rate of the hydrogel is low, physical properties such as viscoelasticity may be degraded after sterilization.
- the crosslinking rate of the hyaluronic acid-alkylenediamine crosslinked product of the present invention refers to the percentage of hyaluronic acid content participating in the crosslinking with respect to the total hyaluronic acid content.
- the crosslinking rate can be adjusted by adjusting the pH at the time of manufacture. Determination of the crosslinking rate can be performed by assays known in the art, such as NMR analysis and TNBS assay (Habeeb, AFSA, Determination of free amino groups in proteins by trinitrobenzenesulfonic acid.Anal. Biochem., 1966. 14: 328-33) This can be done easily.
- the hydrogel of the hyaluronic acid-alkylenediamine crosslinked product of the present invention has a significantly lower decomposition rate than the HA-DVS crosslinked hydrogel using divinyl sulfone (DVS) as a crosslinking agent, and adipic acid dihydrazide ( About twice as much swelling performance as compared to HA-ADH crosslinked hydrogel using ADH) as a crosslinking agent.
- DVD divinyl sulfone
- ADH adipic acid dihydrazide
- Filling composition for reinforcing tissue comprising the hyaluronic acid and alkylenediamine crosslinked hydrogel of the present invention is preferably 1 to 3 (w / w) based on the total weight of the composition of the hyaluronic acid and alkylenediamine crosslinked hydrogel %), Preferably 1.8 to 2.4 (w / w)%. If the hydrogel contains less than 1 (w / w)%, the composition prepared does not have the desired in vivo persistence and protrusion pressure, and if it contains more than 3 (w / w)%, the composition protrudes The pressure may be too high, making injection into the tissue difficult.
- composition of the present invention may further include unmodified (ie, uncrosslinked) hyaluronic acid in addition to the hyaluronic acid-alkylenediamine crosslinked hydrogel.
- Unmodified HA typically uses the same hyaluronic acid used to prepare the hyaluronic acid-alkylenediamine crosslinked hydrogel.
- Unmodified HA acts as a lubricant to lower the pressure over the composition, thereby filling the syringe and injecting the composition into the tissue at a low pressure when injected into the tissue.
- the unmodified hyaluronic acid is included in 0.05 to 1 (w / w)% with respect to the total weight of the composition, more preferably contained in 0.1 to 0.4 (w / w)%.
- the protrusion pressure When included less than 0.05 (w / w)%, the protrusion pressure does not decrease to the desired degree, and when included in excess of 1 (w / w)%, the protrusion pressure is too low, and the composition continues to be pushed out little by little even under one pressure load. It may cause the physical properties to deteriorate, such as excessively low elastic modulus after sterilization.
- composition of the present invention may further include a local anesthetic such as lidocaine in a composition composed of a hyaluronic acid-alkylenediamine crosslinked hydrogel and an unmodified hyaluronic acid, thereby exhibiting anesthetic effect and better physical properties after sterilization.
- a local anesthetic such as lidocaine
- the unmodified HA is mixed with the hydrogel of the hyaluronic acid-alkylenediamine crosslinked product, the extrusion pressure of the composition is lowered, but the elastic modulus (G ′) of the composition after sterilization is lower than that of the unmixed hydrogel.
- lidocaine when lidocaine was mixed, it showed no effect on the protrusion pressure of the composition, but the elastic modulus increased more than 1.28 times after sterilization. That is, by mixing the unmodified HA in the hydrogel of the hyaluronic acid-alkylenediamine crosslinked product of the present invention, the pressure of the composition is lowered and the lidocaine is mixed to prevent the elastic degradation after sterilization by the unmodified hyaluronic acid. have.
- Lidocaine is included in 0.1 to 0.4 (w / w)% based on the total weight of the composition, preferably 0.2 to 0.3 (w / w)%.
- composition consists of pharmaceutically suitable carriers such as water, saline, and the like.
- the composition using the hydrogel of the hyaluronic acid and the alkylenediamine crosslinked product of the present invention has excellent physical properties such as biocompatibility and swelling properties and can be maintained for a long time in a living body, thereby exhibiting an advantageous effect of reducing the number of exchanges.
- the unmodified hyaluronic acid and the composition in which the local anesthetic is further added can be easily sterilized because the physical properties necessary for tissue enhancement such as a decrease in elastic modulus are not degraded even after sterilization. There is an advantage that can be filled and injected into the tissue.
- FIG. 1 is a schematic diagram of the synthesis process of the HA-HMDA crosslinked product of the present invention and the synthesized crosslinked structure.
- Figure 2 shows the modulus of elasticity (G ') after sterilization of the composition comprising the HA-HMDA crosslinked hydrogel of the present invention ( ⁇ : Example 8, ⁇ : Example 9, ⁇ : Example 11).
- Figure 3 shows the degradation effect by hyaluronidase enzyme of the sterilized composition comprising the HA-HMDA crosslinked hydrogel of the present invention and the control product of the restilylene lidocaine in association with the elastic modulus (G ') ( ⁇ Example 11, ⁇ : control).
- Figure 4 is a photograph taken of the degree of dorsal epidermal wrinkle improvement of the wrinkle-induced negative control mouse, restillen administration positive control mouse and the hydrogel administration group mice of Examples 4 and 5 of the present invention observed 11 weeks after the sample injection.
- Figure 5 shows the effect on the tissue of the composition comprising the HA-HMDA cross-linked hydrogel of the present invention in comparison with the control, the tissue samples of the dorsal part of each group of mice stained using H & E and optical microscope The result of the photographing, and the bar indicated is 20 ⁇ m ((A): normal group, (B): wrinkle-induced negative control, (C): restylin administration positive control, (D): HA-HMDA hydro of Example 2 Gel administration group (E): HA-HMDA hydrogel administration group of Example 3, E: epidermis, D: dermis, H: subcutaneous).
- Figure 6 is an image of replica of each group of mice 13 weeks after the sample injection (A: normal group, B: wrinkle-induced negative control, C: restylingen lidocaine administration positive control, D: Example 11 administration group of the present invention).
- Figure 8 shows the effect of the HA-HMDA cross-linked hydrogel sterilized composition of the present invention in comparison with the control, the tissue samples of the dorsal part of each group of mice stained using H & E and photographed with an optical microscope As a result, the indicated bars represent 100 ⁇ m (A: normal group, B: wrinkle-induced negative control group, C: restilendolidocaine dose positive control group, D: Example 11 administration group, E: epidermis, D: dermis, H: subcutaneous) ).
- Hyaluronic acid (HA, manufacturer: Lifecore Core) having a molecular weight of about 230 kDa was completely dissolved in distilled water at a concentration of 1 (w / w)%, and then hexamethylenediamine (HMDA) was added to crosslink by reaction with a carboxyl group. HMDA was added at 72 mol% of the repeat units of HA.
- the carboxyl activator 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) were dissolved in distilled water in an amount of 1.4 times the HA repeating unit, and the HA was And HMDA were added to the mixture.
- the mixed solution was reacted at 37 ° C. for 1 hour for complete crosslinking of the HA-HMDA crosslinked product.
- the pH of the solution was 5.0-5.5.
- the prepared HA-HMDA hydrogel was sealed with a pre-washed dialysis membrane (molecular weight cut-off of 7 kDa) and dialyzed in 0.01 M PBS (phosphate buffered saline, pH 7.4) for 24 hours to maintain residual EDC, HOBt and HMDA. Was removed.
- the degree of crosslinking of the prepared HA-HMDA hydrogel was 8-9%.
- Hyaluronic acid (HA, manufacturer: Lifecore Core) having a molecular weight of about 230 kDa was completely dissolved in distilled water at a concentration of 1 (w / w)%, and then hexamethylenediamine (HMDA) was added to crosslink by reaction with a carboxyl group. HMDA was added at 72 mol% of the repeat units of HA.
- the carboxyl activator 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) were dissolved in distilled water in an amount of 1.4 times the HA repeating unit, and the HA was And HMDA were added to the mixture.
- the mixed solution was reacted at 37 ° C. for 1 hour for complete crosslinking of the HA-HMDA crosslinked product.
- the pH of the solution was 5.0-5.5.
- the prepared HA-HMDA hydrogel was pulverized first, and then passed through a 200 um pore size sieve under constant pressure to obtain a homogeneous hydrogel. 80% ethanol was added to obtain hydrogel powder as a precipitate, 1.3% NaCl solution was added to 100-fold volume and stirred for 1 hour. 80% ethanol was added again to obtain a precipitate, and the mixture was put in 100% ethanol for 10 minutes, and dried under reduced pressure at 40 ° C. for 12 hours to remove residual EDC, HOBt, and HMDA. The degree of crosslinking of the prepared HA-HMDA hydrogel was 8-9%.
- HA-HMDA hydrogel was prepared in the same manner as in Example 1-1 except that HMDA was added at 25 mol% of the HA repeat units.
- the degree of crosslinking of the prepared HA-HMDA hydrogel was 6-7%.
- HA was used in the same manner as in Example 1 except that hyaluronic acid having a molecular weight of about 1,000 kDa (HA, manufacturer: Lifecore Co.) was used at a concentration of 1 (w / w)% and the pH of the solution was 5.5-5.9.
- -HMDA crosslinked was prepared. The degree of crosslinking of the prepared HA-HMDA hydrogel was 11-13%.
- Example except that hyaluronic acid having a molecular weight of about 1,000 kDa (HA, manufactured by Lifecore Co.) was used at a concentration of 1.5 (w / w)%, and the amount of HMDA added was 25 mol% of the repeating unit of HA.
- a HA-HMDA crosslinked product was prepared.
- the pH of the prepared hydrogel was 5.0-5.5.
- the degree of crosslinking of the prepared HA-HMDA hydrogel was 11-13%.
- Hyaluronic acid (HA, manufacturer: Lifecore Co.) having a molecular weight of 1,000 kDa was completely dissolved in water at a concentration of 3 (w / w)%, and then hexamethylenediamine (HMDA) was added to crosslink by reaction with a carboxyl group. HMDA was added at 20 mol% of the repeating units of HA. The pH was adjusted to 6.0-6.5 by addition of 0.25 N NaOH aqueous solution.
- the carboxyl activator 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) were dissolved in distilled water in an amount of 1.0 times the HA repeating unit, and the HA was And HMDA aqueous solution.
- the pH was adjusted to 6.0-6.5 by addition of 0.25 N NaOH aqueous solution.
- the mixed solution was reacted at 45 ° C. for 1 hour for complete crosslinking of the HA-HMDA crosslinked product.
- the prepared HA-HMDA hydrogel was sealed with a pre-washed dialysis membrane (molecular weight cut-off of 7 kDa) and dialyzed in 0.01 M PBS (phosphate buffered saline, pH 7.4) for 24 hours to maintain residual EDC, HOBt and HMDA.
- PBS phosphate buffered saline, pH 7.4
- Hyaluronic acid (HA, manufacturer: Lifecore Co.) having a molecular weight of 1,000 kDa was completely dissolved in water at a concentration of 3 (w / w)%, and then hexamethylenediamine (HMDA) was added to crosslink by reaction with a carboxyl group. HMDA was added at 20 mol% of the repeating units of HA. The pH was adjusted to 6.0-6.5 by addition of 0.25 N NaOH aqueous solution. Carboxyl activator 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) were added to distilled water in an amount of 1.0 times the HA repeating unit for 20 minutes.
- EDC 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide
- HOBt 1-hydroxybenzotriazole
- HA and HMDA aqueous solution were added to the mixed solution of HA and HMDA aqueous solution. Stir at 45 ° C. for 30 minutes. The mixed solution was allowed to stand at 45 ° C. for 10 hours without agitation so that the HA-HMDA crosslinked material was completely crosslinked.
- the prepared HA-HMDA hydrogel was first ground and then passed through a 200 um pore size sieve to obtain a homogeneous hydrogel. 80% ethanol was added to obtain hydrogel powder as a precipitate, 1.3% NaCl solution was added to 100-fold volume and stirred for 1 hour. 80% ethanol was added again to obtain a precipitate, and the obtained hydrogel precipitate was put in 100% ethanol for 10 minutes, and then dried under reduced pressure at 40 ° C.
- HA-HMDA crosslinked hydrogel was prepared in the same manner as in Example 4-1 except for adding to the mixed solution of the mixed aqueous solution.
- An HA-HMDA crosslinked hydrogel was prepared in the same manner as in Example 4-1 except that the mixed solution was left at 45 ° C. for 3, 5, 7, 9 hours without stirring.
- a HA-HMDA crosslinked hydrogel was prepared in the same manner as in Example 4-1 except for homogenizing the hydrogel for 5 minutes at 8,000 rpm with a homogenizer (T-18 basic, IKA) instead of passing through a sieve. .
- HA-HMDA hydrogel was prepared in the same manner as in Example 4-1 except that the prepared HA-HMDA crosslinked product was sealed with a dialysis membrane (7 kDa molecular weight cut-off) and dialyzed in PBS (pH 7.4) for 24 hours. It was.
- the hyaluronic acid having a molecular weight of about 1,000 kDa was used at a concentration of 3 (w / w)%, the amount of HMDA was added using 20 mol% of the repeating unit of HA and the pH was adjusted to 5.5-5.9 by adding 0.25 N NaOH solution.
- a HA-HMDA crosslinked product was prepared in the same manner as in Example 4 except for the above. The degree of crosslinking of the prepared HA-HMDA hydrogel was 10-12%.
- HA-HMDA crosslinking in the same manner as in Example 4, except that hyaluronic acid having a molecular weight of about 1,000 kDa was used at a concentration of 3 (w / w)%, and the amount of HMDA added was 10 mol% of the repeating units of HA. Water was prepared. The degree of crosslinking of the prepared HA-HMDA hydrogel was 5-7%.
- HA-HMDA crosslinking was carried out in the same manner as in Example 4, except that hyaluronic acid having a molecular weight of about 1,000 kDa was used at a concentration of 3 (w / w)%, and the amount of HMDA added was 40 mol% of the repeating units of HA. Water was prepared. The degree of crosslinking of the prepared HA-HMDA hydrogel was 32-35%.
- hyaluronic acid-alkylenediamine hydrogel powder prepared and dried by the method of Example 4-1, 2 mg of hyaluronic acid (HA) having a molecular weight of 1,000 kDa was added and mixed and dissolved in 1 ml of PBS (pH 7.4). After stirring for 5 hours, a composition containing the hydrogel and unmodified hyaluronic acid of the present invention was obtained.
- HA hyaluronic acid
- hyaluronic acid (HA) having a molecular weight of 1,000 kDa was added and mixed and dissolved in 1 ml of PBS (pH 7.4). After stirring for 5 hours, a composition containing the hydrogel and unmodified hyaluronic acid of the present invention was obtained.
- hyaluronic acid-alkylenediamine hydrogel powder prepared and dried by the method of Example 4-1, 2 mg of hyaluronic acid (HA) having a molecular weight of 1,000 kDa and 3 mg of lidocaine were added and PBS (pH 7.4) 1 After mixing in ml, the mixture was stirred for 5 hours to obtain a composition containing the hydrogel, unmodified hyaluronic acid, and lidocaine of the present invention.
- HA hyaluronic acid having a molecular weight of 1,000 kDa
- lidocaine PBS (pH 7.4)
- hyaluronic acid-alkylenediamine hydrogel powder prepared and dried according to the method of Example 4-1, 4 mg of hyaluronic acid (HA) having a molecular weight of 1,000 kDa and 3 mg of lidocaine were added and PBS (pH 7.4) 1 After mixing in ml, it stirred for 5 hours and obtained the composition containing the hydrogel, unmodified hyaluronic acid, and lidocaine of this invention.
- HA hyaluronic acid having a molecular weight of 1,000 kDa and 3 mg of lidocaine
- the pH of the obtained HA / ADH mixed aqueous solution was adjusted to 4.8 using 1N hydrochloric acid aqueous solution, and then stirred thoroughly for 30 minutes. To this was added a 4-fold excess of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC, 0.191 g) powder in a molar ratio with good stirring to activate the carboxyl group of HA.
- EDC 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide
- 1N HCl aqueous solution was added to the aqueous solution and reacted for 2 hours while maintaining the pH at 4.8. After two hours, the reaction was stopped by adding 1N aqueous sodium hydroxide solution to raise the pH to 7.0.
- BS3 Bis [sulfosuccinimidyl] suberate (BS3), a crosslinking agent specific for hydrazide, was also dissolved in PBS and added to HA-ADH solution. At this time, the amount of BS3 added was 20 mol% of HA-ADH hydrazide. After thoroughly mixing the aqueous solution, the reaction was carried out at 37 ° C. for 1 hour to complete the crosslinking reaction to prepare a HA-ADH crosslinked hydrogel.
- BS3 Bis [sulfosuccinimidyl] suberate
- HA-DVS hydrogel was prepared.
- the prepared hydrogel was then sealed with a pre-washed dialysis membrane (molecular weight cut-off of 7 kDa) and dialyzed in PBS for 24 hours to diffuse and release ions (Na + and OH ⁇ ) through the dialysis membrane and sealed HA
- the pH of the -DVS crosslink was neutralized.
- HA-HMDA crosslinking in the same manner as in Example 4 except that hyaluronic acid having a molecular weight of about 1,000 kDa was used at a concentration of 3 (w / w)%, and the amount of HMDA added was 5 mol% of the repeat units of HA. Water was prepared. The cross-linking degree of the prepared HA-HMDA hydrogel was 2-4%, and the viscoelasticity was too low to be used as a tissue filler.
- HA-HMDA crosslinking in the same manner as in Example 4 except that hyaluronic acid having a molecular weight of about 1,000 kDa was used at a concentration of 3.3 (w / w)%, and the amount of HMDA added was 50 mol% of the repeating units of HA. Water was prepared. The degree of crosslinking of the prepared HA-HMDA hydrogel was 40-45%, which was well broken due to the formation of too dry crosslinks, making it difficult to use as a tissue filler.
- the hyaluronic acid degrading enzyme was tested on the hyaluronic acid crosslinked hydrogels of Example 1 and Comparative Examples 1 and 2.
- Example 1 and Comparative Examples 1 and 2 The hyaluronic acid crosslinked hydrogels of Example 1 and Comparative Examples 1 and 2 were packed in the same mass in each vial.
- 0.2 M PBS ( pH 6.2) with 50 U of hyaluronic acid degrading enzyme (hyaluronidase from Streptomyces hyalurolyticus , Sigma-Aldrich) was added. The mixture was reacted at 37 ° C. for a predetermined 48 hours. Thereafter, the supernatant was completely removed and the mass of the remaining hyaluronic acid crosslinked material was measured.
- hyaluronic acid degrading enzyme hyaluronidase from Streptomyces hyalurolyticus
- the degree of decomposition of the crosslinked product was calculated as the mass ratio (%) of the remaining crosslinked product and the original crosslinked product, and the degradation rate (degradation (%)) over time was shown in Table 1.
- Table 1 the HA-DVS crosslinked product of Comparative Example 2 was completely decomposed within about 25 hours, while the hyaluronic acid-alkylenediamine crosslinked hydrogel according to the present invention was partially decomposed after 40 hours. Only it was done. From this, it can be seen that the hyaluronic acid-alkylenediamine crosslinked hydrogel of the present invention has excellent biopersistence superior to commercial hydrogels.
- the hyaluronic acid-alkylenediamine crosslinked hydrogel according to the present invention exhibits a swelling ratio of two or more times higher than that of the HA-ADH crosslinked product of Comparative Example 1 showing a similar decomposition rate. From this, it can be seen that the hyaluronic acid-alkylenediamine crosslinked hydrogel according to the present invention is an excellent tissue enhancing filler which simultaneously exhibits bio-persistence and swelling properties.
- Hyaluronic acid crosslinked hydrogels from Example 1 to Example 7 were packed in the same mass in each vial.
- the mixture was reacted at 37 ° C. for 2 hours.
- 0.8 M potassium borate pH 9.1 was added and then heated at 100 ° C. for 3 minutes.
- Ehrlich's reagent was prepared according to the method of Reissig et al (J. Biol. Chem; 1955; 217: 959-996) to determine the amount of N-acetylglucosamine (NAG) that was degraded.
- the reagent was added to the vial and allowed to react at 37 ° C for 20 minutes. Thereafter, the supernatant was collected by centrifugation, and the amount of NAG in the decomposed crosslinked product was measured by UV at 585 nm.
- Degradation degree of the crosslinked product was set to 100% decomposition rate of Example 6, the decomposition rate of the remaining examples are shown in Table 2 below.
- the crosslinking rate was affected by the molecular weight, initial concentration and pH of HA.
- This experimental example was carried out to confirm the optimal hydrogel preparation concentration of the composition comprising the hyaluronic acid crosslinked hydrogel of the invention.
- the hyaluronic acid crosslinked hydrogel of Example 4-1 was dissolved in PBS (pH 7.4) at concentrations of 1%, 1.5%, 2%, and 2.4%, respectively, and stirred for 5 hours, and these were filled in a syringe, and 121 ° C and 1.5 degrees. After sterilization atm for 20 minutes, the degradation rate, protrusion pressure, and modulus of elasticity (G ′) of the hyaluronic acid degrading enzyme were measured for this sterilized composition.
- HA hyaluronic acid
- PBS pH 7.4
- the stirred composition was filled in 3 ml glass syringes with the same mass, followed by wet sterilization (121 ° C., 1.5 atm, 20 minutes), and the average particle size and protrusion pressure for each sterilized composition were measured and shown in Table 4 below.
- the average particle size and distribution were measured using a Malvern Mastersizer 2000 (Malvern Instruments LTD, Worcestershire, UK) equipment and measured using 0.9% NaCl solution as a dispersion solvent. Protruding pressure was measured up to 6 mm at a constant speed of 1 mm / min using a 27 gauge and 30 gauge needle and EZ-S SHIMADZU.
- the protrusion pressure was affected by the composition ratio of the unmodified HA. That is, it was confirmed that as the content of the unmodified HA in the composition increases, the unmodified HA acts as a lubricant, thereby reducing the protrusion pressure. According to the gauge of the needle used, the difference between the 30 gauge and the 27 gauge was approximately 1.6 times for the 3 ml syringe. On the other hand, the addition of lidocaine had little effect on the protrusion pressure. In the case of Examples 10 and 12 having a high content of unmodified HA in the composition, the protrusion pressure was too low, so that the composition continued to flow even after stopping after applying a constant force.
- Example 4-9 homogenized with a homogenizer without passing through a sieve had a higher protrusion pressure than that when homogenized with a sieve.
- a hydrogel with a wide particle distribution was obtained, and it was considered that the protrusion pressure was increased due to the irregularly sized hydrogel particles.
- HA hyaluronic acid
- PBS pH 7.4
- the stirred composition was filled into 3 ml glass syringes in equal mass and then wet sterilized (121 ° C., 1.5 atm, 20 minutes), and the method of Ghosh et al (Biomacromolecules 2005; 6: 2857-2865) was applied to each sterilized composition. Rheological tests were conducted.
- the elastic modulus is affected by the composition ratio of the unmodified HA.
- the modulus of elasticity of the sterilized composition increased by 25.6% compared to the case where only HA was added. It was confirmed.
- the modulus of elasticity was slightly reduced (4.9%) compared with the case of homogenization with a sieve. 2 shows the modulus of elasticity of 0.1-20 Hz of the sterilized compositions of Examples 8, 9 and 11.
- the elastic modulus after sterilization was measured for the compositions of Examples 4-1 to 4-4.
- the stirring time of EDC / HOBt which is a reaction aid, was changed to 20, 30, 40, and 60 minutes, respectively.
- Each prepared hydrogel powder was added to PBS (pH 7.4) at a concentration of 20 mg / ml, stirred for 5 hours, and then wet sterilized (121 ° C., 1.5 atm, 20 minutes) in a 3 ml glass syringe.
- PBS pH 7.4
- 4-cm 2 ° -cone and plate geometry, measured at 0.1-20 Hz in 1% strain and oscillation mode, measured at 3 Hz
- the coefficient (G ′) values are shown in Table 6 below. The deviation of the instrument was ⁇ 10% and the experiment was conducted at 25 ° C.
- the preparation of the hyaluronic acid cross-linked hydrogel was found to be affected by the time until the reaction dosing time after dissolving in an aqueous solution of EDC / EDU.
- Hyaluronic acid cross-linked hydrogel prepared by dissolution to charge within 20-30 minutes is similar to the elastic modulus after sterilization.
- the hydrogel produced from dissolution to infusion for 40 minutes or more was degraded after sterilization, and at 60 minutes or more, hydrogels having a low elastic modulus such as water were prepared after sterilization. From these results, it can be seen that the time from dissolution to dosing of EDC / HOBt in the preparation of the hyaluronic acid-alkylenediamine crosslinked hydrogel is a very important factor.
- the elastic modulus after sterilization of the compositions of Examples 4-1, 4-5 to 4-8 Measured.
- the hydrogels of Examples 4-1 and 4-5 to 4-8 were prepared by varying the additional standing time for the complete crosslinking reaction of the hyaluronic acid crosslinking to 3, 5, 7, 9, and 12 hours.
- Each prepared hydrogel powder was added to PBS (pH 7.4) at a concentration of 20 mg / ml, stirred for 5 hours, and then wet sterilized (121 ° C., 1.5 atm, 20 minutes) in a 3 ml glass syringe.
- PBS pH 7.4
- 4-cm 2 ° -cone and plate geometry, measured at 0.1-20 Hz in 1% strain and oscillation mode, measured at 3 Hz
- the coefficient (G ′) values are shown in Table 7 below. The deviation of the instrument was ⁇ 10% and the experiment was conducted at 25 ° C.
- the control limit of the hydrogel of Example 4-10 obtained by removing the unreacted substance by using dialysis membrane or the hydrogel of Example 4-1 removed using ethanol and buffer was controlled to 2 ppm or less. It was confirmed.
- the detection limit was 2 ppm or less in the hydrogel of Example 4-1, but in Example 4-10 obtained by removing the unreacted substance using a dialysis membrane, about 10 times as much HOBt was detected. This may be because HOBt tends to be more soluble in organic solvents, making it difficult to purify by dialysis.
- the hyaluronic acid crosslinked hydrogel sterilized composition of the present invention was evaluated by comparing the sensitivity to hyaluronic acid degrading enzyme with a value of G 'and comparing with restilylene lidocaine, a filler for enhancing tissue, which is commercially available.
- the sterile composition and restylene lidocaine of Example 11 of the present invention were dispensed in 800 ml in 1.5 ml test tubes.
- tissue enhancement effect and biocompatibility of the hyaluronic acid crosslinked hydrogel of the present invention were evaluated in comparison with Restylane ® , a filler for improving wrinkles on the market.
- the hyaluronic acid crosslinked hydrogel of the present invention is the hyaluronic acid crosslinked hydrogels of Examples 2, 3, 4 and 5 of the present invention, respectively, in a homogeneous group (T-18 basic, IKA, Tokyo, Japan) at 5 Prepared by homogenization for minutes, and 0.4 ml each was injected through a 30 gauge syringe needle into a tattooed rectangle in the dorsal subcutaneous layer of the wrinkle-inducing mouse.
- the wrinkle improvement area over time is shown in Table 8 below.
- Table 8 the group treated with Restylane ® after the induction of wrinkles as a positive control group, the HA-HMDA crosslinked hydrogel of Example 2 of the present invention, and the HA-HMDA crosslinked hydrogel of Example 3 were treated.
- mice showed a marked improvement in wrinkles compared to the wrinkle-induced negative control mice treated with nothing after the induction of wrinkles.
- FIG. 4 shows the hairless mice treated with wrinkle-induced group as a negative control after 11 weeks, hairless mice treated with Restylane ® after the induction of positive control, and hairless mice treated with HA-HMDA hydrogels of Examples 4 and 5 after wrinkles. Photograph showing the condition of the dorsal epidermis. As can be seen from Table 8 and Figure 4, the composition comprising the HA-HMDA hydrogel of the present invention showed a better wrinkle improvement effect than Restylane ® .
- H & E hematoxylin-iosin staining 12 weeks after hyaluronic acid crosslinked hydrogel administration. Skin samples were taken from each mouse and fixed in 10% by volume buffered formaldehyde, dehydrated with ethanol, then stored in paraffin to make samples, which were cut into 4 ⁇ m sections and stained with H & E. 5 shows the results of photographing the stained samples using an optical microscope. As shown in Figure 5, the mice administered the hydrogels of the present invention did not show any inflammatory response as the control mice did not cause wrinkles (in H & E, hematozain was blue Stained with iosin, which is stained red with hematoxylin as a contrast dye).
- the wrinkle-induced negative control (5B) compared to the normal group (5A) it can be seen that the epidermis (E) is flattened, the dermis (D) is thick and rough due to the skin damage caused by wrinkles.
- the test group injected with the positive control group administered with the Wrinkle-induced negative control group, Restylane ® remarkably expanded the lumen of the subcutaneous tissue, while regenerating by injecting the HA-HMDA crosslinked hydrogel of Example 3
- the lumen of the subcutaneous tissue was regenerated in almost the same form as the lumen of the control subcutaneous tissue that did not cause wrinkles.
- tissue enhancement effect and biocompatibility of the hyaluronic acid crosslinked hydrogel composition of the present invention were evaluated in comparison with Restylane ® Lidocaine, a filler for improving wrinkles on the market.
- Example 11 of the present invention Three days after the application of the wrinkle causing agent was stopped, 0.4 ml of the hyaluronic acid crosslinked hydrogel composition of Example 11 of the present invention was injected through a 3 ml syringe and a 27 gauge needle into a tattooed rectangle of the dorsal subcutaneous layer of the wrinkled mouse. .
- 0.4 ml of Restylane ® Lidocaine (manufactured by Q-Med AB; 20 mg / ml) was injected using a 27-gauge needle.
- H & E hematoxylin-iosin staining 14 weeks after hyaluronic acid crosslinked hydrogel administration. Skin samples were taken from each mouse and fixed in 10% by volume buffered formaldehyde, dehydrated with ethanol, then stored in paraffin to make samples, which were cut into 4 ⁇ m sections and stained with H & E. The result of photographing the stained sample using an optical microscope is shown in FIG. Mice administered with the sterilized hydrogel composition of Example 11 of the present invention did not show any inflammatory response as in the normal group mice (for H & E, hematoxylin is stained blue in the inflammatory reaction, Io God is dyed red as a contrast dye with hematozain).
- Wrinkle-induced negative control and restylingulidocaine-positive control showed some inflammatory responses in the dermis, but the hydrogel-administered group of the present invention did not have any hemolysis or inflammatory response as the normal group, and dermal tissue was regenerated.
- the thickness of the regenerated dermis was equivalent to the normal control and the positive control group receiving restylene lidocaine. This increase in dermal thickness effectively contributes to tissue enhancement.
- the test group injected with the anti-wrinkle negative control group, the positive control group administered with restylene lidocaine significantly expanded the lumen of the subcutaneous tissues, whereas the regenerated subcutaneous tissues were injected by injecting the sterilized composition of Example 11. The lumen was regenerated in almost the same way as the subcutaneous lumen of the normal group.
- the hyaluronic acid-alkylene diamine crosslinked hydrogel and the sterilized hydrogel of the present invention show excellent biocompatibility, and have excellent tissue enhancement due to proper swelling and regeneration of dermis and subcutaneous tissue. Effect), and since it lasts much longer in vivo than a commercial tissue filler, it can be effectively used as a tissue filler.
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Abstract
Description
Claims (21)
- 하기 식 1의 히알루론산 및 알킬렌디아민 가교물의 하이드로겔을 포함하는 조직 증강용 충전 조성물;[식 1][HA]m-C(O)-NH-R1-NH-C(O)-[HA]n(식에서, HA는 카르복실기 하나를 제외한 히알루론산 또는 그의 염을 나타내고, R1은 히드록시, C1-C6 알킬, 또는 C1-C6 알콕시로 치환되거나 비치환된 C3-C10 알킬렌기이며, m 및 n은 10,000 ~ 4,000,000의 정수이다).
- 제1항에 있어서, 미변형 히알루론산을 더 포함하는 조성물.
- 제2항에 있어서, 리도카인을 더 포함하는 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 히알루론산의 분자량이 20,000 달톤 내지 4,000,000 달톤인 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 알킬렌디아민이 헥사메틸렌디아민인 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 히알루론산 및 알킬렌디아민 가교물은 pH 5.5 내지 6.5에서, 카르복실기 활성화제와 펩티드 결합 촉진제의 존재하에 히알루론산을 히드록시, C1-C6 알킬, 또는 C1-C6 알콕시로 치환되거나 비치환된 C3-C10 알킬렌 디아민 화합물과 반응시켜 제조한 것인 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 가교물 하이드로겔의 가교율이 5 ~ 35 %인 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 히알루론산 및 알킬렌디아민 가교물 하이드로겔은 조성물 총 중량에 대하여 1 ~ 3 (w/w)%로 포함되는 조성물.
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 피부의 주름을 제거 또는 개선시키기 위한 것인 조성물.
- 제1항 내지 제3항 중 어느 한 항에 있어서, 볼, 입술, 가슴 및 엉덩이로부터 선택되는 신체부위의 부피를 확대시키기 위한 것인 조성물.
- (1) 카르복실기 활성화제와 펩티드 결합 촉진제의 존재하에 히알루론산을 히드록시, C1-C6 알킬, 또는 C1-C6 알콕시로 치환되거나 비치환된 C3-C10 알킬렌 디아민 화합물과 반응시켜 히알루론산 및 알킬렌디아민 가교물 하이드로겔을 제조하는 단계;(2) 제조된 하이드로겔을 균질화하는 단계; 및(3) 미반응물을 제거하는 단계를 포함하는 제1항의 조성물의 제조방법.
- 제11항에 있어서, 30분 이내에, 카르복실기 활성화제와 펩티드 결합 촉진제를 물에 용해시키고 히알루론산과 알킬렌 디아민 화합물 혼합물에 첨가하는 것인 방법.
- 제11항에 있어서, 상기 제1단계에서 30℃ 내지 50℃에서 9시간 이상 교반없이 방치하여 히알루론산 및 알킬렌디아민 가교물 하이드로겔을 제조하는 것인 방법.
- 제11항에 있어서, pH 5.5 내지 6.5에서 히알루론산 및 알킬렌디아민 가교물 하이드로겔을 제조하는 것인 방법.
- 하기 식 1의 히알루론산 및 알킬렌디아민 가교물 하이드로겔을 포함하는 조직증강용 충전제;[식 1][HA]m-C(O)-NH-R1-NH-C(O)-[HA]n(식에서, HA는 카르복실기 하나를 제외한 히알루론산 또는 그의 염을 나타내고, R1은 히드록시, C1-C6 알킬, 또는 C1-C6 알콕시로 치환되거나 비치환된 C3-C10 알킬렌기이며, m 및 n은 10,000 ~ 4,000,000의 정수이다).
- 제 15항에 있어서, 상기 히알루론산 및 알킬렌디아민 가교물은 히알루론산을 카르복실기 활성화제와 펩티드 결합 촉진제의 존재하에 히드록시, C1-C6 알킬, 또는 C1-C6 알콕시로 치환되거나 비치환된 C3-C10 알킬렌 디아민 화합물과 반응시켜 제조한 것인 조직증강용 충전제.
- 제15항에 있어서, 상기 알킬렌디아민이 헥사메틸렌디아민인 조직증강용 충전제.
- 제15항에 있어서, 상기 히알루론산의 분자량이 10,000 달톤 내지 4,000,000 달톤인 조직증강용 충전제.
- 제15항 내지 제18항 중 어느 한 항에 있어서, 상기 가교물의 가교율이 5 ~ 35 %인 조직증강용 충전제.
- 제15항 내지 제18항 중 어느 한 항에 있어서, 피부의 주름을 제거 또는 개선시키기 위한 것인 조직증강용 충전제.
- 제15항 내지 제18항 중 어느 한 항에 있어서, 볼, 입술, 가슴 및 엉덩이로부터 선택되는 신체부위의 부피를 확대시키기 위한 것인 조직증강용 충전제.
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US13/809,432 US9017712B2 (en) | 2010-07-12 | 2011-07-11 | Filler composition for tissue reinforcement |
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CA2805008C (en) | 2015-05-12 |
KR20120006451A (ko) | 2012-01-18 |
US20130203856A1 (en) | 2013-08-08 |
US9017712B2 (en) | 2015-04-28 |
WO2012008722A3 (ko) | 2012-05-03 |
JP2013530785A (ja) | 2013-08-01 |
CA2805008A1 (en) | 2012-01-19 |
KR101400907B1 (ko) | 2014-06-10 |
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