WO2011096659A2 - Récipient de culture de microorganismes ou de cellules - Google Patents

Récipient de culture de microorganismes ou de cellules Download PDF

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Publication number
WO2011096659A2
WO2011096659A2 PCT/KR2011/000440 KR2011000440W WO2011096659A2 WO 2011096659 A2 WO2011096659 A2 WO 2011096659A2 KR 2011000440 W KR2011000440 W KR 2011000440W WO 2011096659 A2 WO2011096659 A2 WO 2011096659A2
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WO
WIPO (PCT)
Prior art keywords
culture vessel
culture
elastic membrane
container body
elastic
Prior art date
Application number
PCT/KR2011/000440
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English (en)
Korean (ko)
Other versions
WO2011096659A3 (fr
Inventor
전민용
Original Assignee
Jeon Min-Yong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jeon Min-Yong filed Critical Jeon Min-Yong
Publication of WO2011096659A2 publication Critical patent/WO2011096659A2/fr
Publication of WO2011096659A3 publication Critical patent/WO2011096659A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible

Definitions

  • the present invention relates to a microorganism or a cell culture vessel, and to a culture vessel that blocks invasion of bacteria or various bacteria and reduces contamination by using an elastic membrane in a culture propagation apparatus such as a microorganism or a living tissue.
  • aseptic culture of all animal and plant cells and tissues is injected into a sterile culture vessel, the sterile cells and tissues are healed therein, and then filled with a stopper to prevent external contamination. It is a so-called subculture that is cultured for a period of time, and when the culture is grown after a certain period of time and the space in the culture container is physically insufficient, or when the amount or moisture of the culture medium is depleted, the culture is changed to fresh culture. It is done periodically.
  • Stem cells include embryonic stem cells from early blastocysts (Blstocyst) and adult stem cells from adult or placenta after the developmental process. There are three main methods of obtaining these adult stem cells: first through bone marrow, second through umbilical cord blood, and then peripheral blood. easily obtained through mesenchymal blood. In addition, techniques for obtaining adult stem cells from adipocytes or other tissues of the body have been researched and developed.
  • stem cells are collected in such a small number, they make a large amount of stem cells through culture and inject them into tissues that require cell regeneration such as skin, cardiovascular, and organ tissues.
  • the collected stem cells are inoculated and cultured in culture vessels of various shapes and types by traditional culture methods.
  • the conventional method of culturing strains or cells is to sterilize the inoculation needle with an alcohol lamp on a work bench that is blocked from outside air called cleanbench in a laboratory, and then remove the lid or stopper of the culture vessel containing the strain or biological tissue to be inoculated. After opening, use a needle to remove some of the strains, cells, etc., the chalena triangle flask containing the culture solution, or the culture vessel with the lid or stopper of the culture vessel or tissue culture well or plate open. After plating on the culture medium, the inoculation process is performed by sealing or covering the lid or stopper of the culture vessel.
  • Inoculation and incubation process of the microbial strain of the conventional method sterilize the tweezers or inoculation needle in the flame of the alcohol lamp in the open space, open the lid (2) of the cell (1) containing the cell culture medium
  • the strain in (17) was buried in the inoculation needle (3), and then the lid (2) of the casserole (1) to be cultured was opened to spread the strain on the inoculation needle (3) onto the medium (17).
  • the lid 2 is covered with a tape sealing process.
  • Subsequent cultures are incubated for a certain period of time in an incubator capable of controlling the environment, that is, the temperature or the atmosphere of the stem cells to be grown, and the medium is replaced or subcultured as necessary. The process is repeated.
  • microbiological laboratories are equipped with a microbiological aseptic workbench, which occupies a large space called a clean bench, and even though work is performed in such a space, work failure occurs due to contamination of the culture proliferation caused by the contamination of the microorganisms described above. have.
  • Patent Publication No. 2001-0029122 discloses an animal and plant cell culture vessel for preventing microbial contamination, which is equipped with a special micro filter that does not pass external pollutants and can easily exchange gas to prevent microbial contamination in the culture process. Suggesting.
  • Patent Publication No. 1020040051602 discloses a container and a cap for a container provided with a filter medium for aeration of a container and a filter medium for aeration, in order to prevent entry of germs or mites into a medium in a sterilized culture container.
  • Ventilation filter media for Iii) and a cultivation vessel with such media and a cap for the cultivation vessel is shown.
  • This cultivation bottle is provided with a bottle main body and the cap attached to an opening part.
  • the cap has a cap body and a filter medium for ventilation.
  • the cap body is provided with a hole.
  • a filter medium for aeration a porous membrane made of polytetrafluoroethylene and having micropores having a diameter of 0.1 to 50 ⁇ m or less is provided.
  • clean benches which are microbiological aseptics, are equipped with a device that blocks the inflow of external air and prevents and sterilizes microorganisms in the air or the fallout of its spores.
  • this small medical institution there is a difficulty that cannot be provided.
  • a medical institution, a microbiological laboratory, or a bio-industrial equipped with such equipment is present, contamination of the tissue culture during microbial or stem cell cultivation depending on the skill of the operator or the degree of microbial contamination in the clean bench. This is inevitable, which wastes time and increases work cost.
  • the present invention it is possible to improve the convenience of operation in the apparatus for inoculating and cultivating animal or plant tissues, strains and stem cells, to prevent contamination of microorganisms that may occur in the working process, and to cultivate economically and efficiently cells.
  • the present invention is different from the conventional method of opening and closing a container containing a culture medium or a strain and a flora and fauna tissue, and contaminating cells and microorganisms due to the inflow of microorganisms present in the air by injecting or withdrawing the contents without opening and closing the culture vessel. To prevent it.
  • the present invention is to provide a culture vessel capable of performing aseptic microbial and cell manipulation even in the absence of a clean bench.
  • the present invention is to provide a culture vessel for storing the culture medium in a powder state in order to increase the shelf life of the culture medium container.
  • the present invention is intended to be able to quantitatively inject a strain or a solution in a closed state through the elastic membrane of the culture vessel with the tip of the pointed micropipette in order to inject the correct amount into the culture vessel.
  • the present invention exports the gas generated from the cells or strains in culture, the required gas is introduced into the culture vessel to block the infiltration of microorganisms or their spores that cause contamination of the culture, the external contaminating microorganisms or their spores It is prevented from being penetrated into the culture vessel by being buried on the surface of the operating device such as the needle inserted into the microorganism or the inoculation needle micropipette tip, and even though the microorganism penetrated through the primary elastic membrane is blocked by the secondary elastic membrane. Since the contact with the sterile layer is limited is to provide a culture vessel that is naturally inhibited and killed.
  • a rubber, silicone, or the like is used in the body or stopper of a culture vessel such as a culture medium, an enzyme, a reagent, a culture medium or a container containing, collecting or culturing microorganisms or biological tissues, that is, a tissue culture well or cave, an Erlenmeyer flask, a culture bottle, or a plate.
  • a culture vessel such as a culture medium, an enzyme, a reagent, a culture medium or a container containing, collecting or culturing microorganisms or biological tissues, that is, a tissue culture well or stii, an Erlenmeyer flask, a culture bottle, or a plate.
  • Forming an elastic membrane or elastic stopper formed by using the same, and using the operating mechanism, such as inoculation needle, needle or scraper microneedle tip passing through the elastic membrane or elastic stopper is collected and collected by the culture solution, bacteria, biological tissues, etc. Sealing operation of the culture medium or animal or plant tissues, strains
  • the present invention is a culture vessel for culturing a strain or cells, comprising a container body having a through hole on one surface, and an elastic membrane made of an elastic body blocking the through hole of the container body.
  • the elastic membrane is fixedly attached around the through-hole or coupled in a detachable stopper form, or the elastic membrane is a laminated elastic membrane laminated with two or more elastic membranes, a sterilization layer between the elastic membrane of the laminated elastic membrane Is formed.
  • a through hole is formed in a part of the container body, and a filter is attached to block the through hole.
  • the elastic membrane of the present invention has a filter that blocks a part of the through hole and blocks the remaining unblocked part of the through hole.
  • the culture vessel of the present invention has a three-dimensional structure according to the type of culture strain or cells that have three or more small chalena or cell culture wells formed inside the container body, and three-dimensional uneven treatment on the inner bottom of the container body.
  • One containing a three-dimensional cell culture artificial material inside the container body, coated with a material that aids the attachment of cells to the inner surface of the container body, or containing a solid or liquid medium inside the container body will be.
  • the elastic membrane serves as a filter and the elastic membrane at the same time, or a partition wall is formed on the bottom of the container body.
  • the tip of the micropipette for injecting or withdrawing a certain amount of material through the elastic membrane has a sharply shaped tip, or the needle portion of the micropipette tip is formed of a metal material and attached to the micropipette or the pipette.
  • the part is formed of synthetic plastic.
  • the present invention is to keep the culture solution in a powder or dried state in the container body, sterile distilled water, physiological saline, or other solvent through the elastic membrane immediately before inoculating microorganisms or cells to be used.
  • Stem cell culture vessel to prevent the contamination of microorganisms according to the present invention has the following effects.
  • the tip of the tip of the micropipette can penetrate the elastic membrane of the culture vessel and quantitatively inject the strain or solution in a closed state.
  • the gas generated from the cells or strains in culture is discharged, and the required gas flows into the culture vessel, and at the same time a filter is formed to block the infiltration of microorganisms or spores that cause contamination of the culture, thereby preventing contamination in microbial culture. Block it.
  • 1 is an exemplary view showing a process of a conventional microbial operation
  • FIG. 2 and 3 is a view showing a first embodiment of the culture vessel having an antifouling elastic membrane of the present invention.
  • components or parts having the same function are denoted by the same reference numerals for convenience of understanding.
  • the present invention forms a through hole in one side of the lid 2 or the body 16 of the mast 1, which is a culture vessel, and is closed with an elastic membrane 4. It is formed in at least one through-hole 5 formed in the body 16 or the lid 2 of a synthetic plastic or glass material.
  • the medium 17 is installed in the body 16.
  • the elastic membrane 4 is usually made of elastic material such as rubber, silicone, or the like, and is bonded around the through hole 5 formed on either side of the body 16 or the lid 2 of the culture vessel. The edge portion of the elastic membrane is fused with the container by heat, ultrasonic waves, or high frequency fusion to form an adhesive layer 6.
  • the material of the elastic membrane is not limited to rubber or silicone, and the elastic membrane 4 may be made of a thin film as a material having elastic force. Even if a puncture occurs by the needle 8 or the like, the puncture is closed again to close the culture vessel. You can make it using recoverable materials.
  • the elastic membrane 4 can also be made of synthetic plastics that act as filters.
  • the elastic membrane 4 may be formed to have a composite function in which rubber or silicon is coated on a portion thereof.
  • the elastic membrane 4 may be formed in a fine fiber shape so as to perform the role of the filter 15 at the same time.
  • FIG. 4 shows another embodiment of the present invention, showing that the elastic membrane 4 is coupled to the through hole 5 of the lid 2 in the form of a stopper 7.
  • the elastic membrane 4 may be coupled to the through hole of the container body 16 or the lid 2 and fitted into a hole formed in a portion of the elastic membrane 4 fixedly attached to the through hole 5. May be combined.
  • the stopper 7 may be detachably coupled to the lid 2 or a part of the container body 16 and a part of the stopper 7 in a fused form, or the stopper 7 may be attached to a part of the elastic membrane 4. A part of) may be formed by fusion.
  • the same function as in the first embodiment except that the plug can be opened and closed.
  • FIG 5 is a view showing another embodiment of the present invention, in which the plurality of planets 1 are disposed in the body 16 of the culture vessel, and the lid covering the body corresponds to the position of each planet. Stopper 4 is formed in the position.
  • FIG. 6 is a view showing another embodiment of the present invention, in which the entire opening portion is sealed with the elastic membrane 4 instead of the lid of the culture vessel body 16.
  • the elastic membrane 4 instead of the lid of the culture vessel body 16.
  • it is provided with the necessary medium 17 and various components necessary for cultivation and then sealed with the elastic membrane 4.
  • it is necessary to make the jaw 12 to put the adhesive area with the elastic membrane 4 on the edge and to form the adhesive layer 6 thereon. good.
  • the elastic membrane 4 has a needle 1 through which the needle 8, the inoculation needle 3, or the tip 9 of the micropipette 19 is sharply formed like a needle. Inoculate strains, animals and plants and stem cells into the culture medium (17) or supplement the culture solution, etc., and remove the holes so that the perforated holes are elastically closed. Function.
  • the end cross section of the conventional micropipette tip 9 is rounded or cut at an angle of 90 degrees, in the present invention, the micropipette tip 9 penetrates the elastic membrane 4 to the plin 1
  • the tip is sharply inclined so as to penetrate the inside, like a syringe needle, so that the elastic membrane 4 can be easily penetrated.
  • the material of the micropipette tip 9 may be made of synthetic plastic or metal material, or the end portion penetrating and penetrating the elastic membrane 4 is formed of metal and inserted into the micropipette 19 or the pipette.
  • the losing part may be formed of synthetic plastics such as PE, PP, and PET.
  • the groove 10 is formed so that the rubber or silicone ring 11 is fitted to the side edge of the micropipette or the pipette, and the sealing ring 11 is fitted to engage the micropipette tip 9 so as to strongly bind the pipette or the micropipette. So that it does not fall easily during microbial manipulation.
  • Penetrating the elastic membrane 4 is not limited to the needle (8) inoculation needle (3) micropipette tip (9) described above, in the culture vessel such as Georgias (1), Erlenmeyer flasks, culture bottles, tissue culture wells, etc. Instruments that are deemed necessary for a variety of manipulation operations, such as micro-tweezers, micro-scissors, knives, scrapers, etc., are operated without opening the cap (7) or lid (2) of the culture vessel outside the culture vessel. Any apparatus that can be used for the work can be used.
  • the instruments used for the operation work are put into the container, and inoculated and injected with the cells of the required strain or animal or plant or the necessary culture solution, cell differentiation inducing solution, enzyme reagent, or the like.
  • the microorganisms and stem cell manipulation operations can be performed in conventional culture vessels or culture vessels by performing an operation for separating and transplanting cultured cells or strains and replenishing the necessary culture solution in a closed culture vessel. Compared to contaminated through the open and close operation of the open and closed process, the contamination is significantly reduced, eliminating the cost and waste of time, and also reducing the need for a clean bench that takes up a large amount of space. It is very convenient to use in hospitals, schools, laboratories, etc. All.
  • Figure 7 is a view showing another embodiment of the present invention, the culture vessel body 16 is used to have a form such as a bottle, the lid 2 is a structure to be sealed using a bottle cap.
  • the through-holes are formed on one side of the body, and then the elastic membrane 4 is attached, and the through-holes are formed on one side of the body for the distribution of gas necessary for cultivation, and the filter 15 is attached.
  • the filter may be a microfilter that allows gas and gas generated at the time of cultivation to be used for the strain or cell to be cultured.
  • the medium 17 is installed on the lower side to which the elastic membrane and the filter are not attached. This embodiment shows that the elastic membrane 4 can be formed in the body 16 of the culture vessel as well as the lid 2 according to the shape and use of the culture vessel.
  • the culture vessel has a body 16 having a plurality of culture cell castings and a lid 2 formed with an elastic membrane over a wide range. This lid is provided with the filter 15 superimposed on the elastic membrane 4.
  • the culture vessel may be a round or square dish as well as a triangular flask or bottle type or a type of cell culture plate in which a plurality of compartment cells or wells are formed.
  • the inner surface of the culture vessel may be coated with a material that helps the adhesion of the cells, it may be in the form of a culture vessel to induce three-dimensional cell proliferation by putting a three-dimensional artificial material inside.
  • the elastic membrane 4 may be formed on the lid 2 of the double culture vessel in which a plurality of planets 1 are disposed, and the medium 17 of the liquid or powder may be pre-injected.
  • a culture vessel may form a ring of silicone or rubber material between the culture vessel and the lid 2 in order to increase the sealing effect between the body 16 and the lid 2, and the screw or coupling formed on the body and the lid 2
  • the jaws can be attached to each other.
  • a culture vessel may be in the form of a plurality of the above-described cell culture wells, or alternatively, may be in the form of a culture vessel containing a plurality of containers.
  • the culture may be well performed by applying electric energy or magnetic energy from the outside of the culture vessel to the inside of the vessel.
  • a culture medium container for storing the culture medium which affects the cell growth of microorganisms or animals or plants, differentiation culture medium which induces differentiation of various cells, enzymes and reagents, etc. It may be applied to the stopper portion of the reagent vessel and formed.
  • a stopper formed of a material such as plastic or glass is contained in a culture container containing a conventional culture solution, there is a possibility of causing microbial contamination during the opening and closing of the stopper to obtain its contents, and a reagent containing a liquid enzyme or reagent. The same holds true for containers.
  • the lyophilized medium 17 component in powder form may also be applied.
  • the culture vessel or the reagent vessel sealed with the stopper 7 formed with the elastic membrane 4 of the present invention can be used to remove the contents using the needle 8 or the tip 9 of the micropipette without opening the stopper 7. It is possible to reduce the microbial contamination through such a closed operation process because it can be applied or injected into the culture vessel, or on the contrary to inject the required solvent components in the culture vessel.
  • the elastic membrane 4 is formed on the film-shaped filter 15 attached to the adhesive layer 6 around the through hole 5 formed in the lid 2.
  • a filter 15 is attached for the release of the gas generated during the at least one microbial metabolism of the culture vessel or the body of the culture vessel and the growth of the flora and fauna and the introduction of the required gas.
  • the filter 15 is formed of a material having a permeability enough to block the influx of microorganisms or spores of the pollutant and to distribute the gas generated during the growth and cultivation of cultured cells or strains.
  • a portion of the filter 15 may be formed by coating the elastic membrane 4.
  • a membrane made of a material having gas permeability and elastic force at the same time may be formed as the elastic membrane 4 or the filter 15 to form part or the whole of the culture vessel.
  • FIG 11 shows another embodiment of the present invention.
  • the lid 2 portion of the culture vessel containing the medium 17 is fused or adhered to the body 16 of the container by the elastic membrane 4 as a whole.
  • the elastic membrane 4 is attached to the jaw 12 formed on the body 16, the elastic membrane through which the operating mechanism, such as the needle (8), inoculation needle (3), micropipette tip (9) ( 4)
  • the second elastic film 13 is added to the site to form a double. It is better to form a film coated with a disinfectant in the double layer of elastic membrane to disinfect the needle entering and exiting the layer.
  • the elastic membrane 4 through which the operating mechanisms such as the needle 8, the inoculation needle 3, and the micropipette tip 9 penetrates is doubled, and microorganisms that may be buried on the surface of the operating mechanism, Spores, etc. are primarily blocked by the external second elastic film 13, or the remaining spores or microorganisms passing therethrough are once again blocked by the elastic membrane 4 formed below.
  • the elastic membrane 4 even if microorganisms infiltrated through the first elastic layer 13 by the sterilization layer 14 provided between the double elastic membranes 4 and 13 exist, they are blocked by the elastic membrane 4 and contaminated with limited contamination. Since the microorganisms come into contact with the bactericidal layer, their activity is naturally inhibited and killed.
  • This structure is to inject a certain amount of medium (17) into the culture vessel automatically in the factory for the efficiency of mass production, and the second elastic membrane ( When the edge portion of the elastic membrane 4 having 13) is fused or bonded, production efficiency is improved.
  • the culture vessel thus formed has the effect of blocking the contaminating microorganisms close to perfection, thereby reducing the space of the clean bench which occupies a large volume. It can be done.
  • microorganism or cell culture apparatus for preventing the microbial contamination of the present invention is not limited to the above-described embodiment, anyone of ordinary skill in the art without departing from the gist of the present invention and various modifications can be carried out It will be referred to the technical scope of this invention to the extent possible.
  • the present invention relates to a microorganism or a cell culture vessel, and provides a culture vessel that blocks invasion of bacteria or various bacteria and reduces contamination by using an elastic membrane in a culture propagation device such as a microorganism or a living tissue. It can be used in the medical industry, various laboratories, or the manufacture of medical devices. Culture growth of biological materials is the most fundamental work of bio industry such as identification and amplification of DNA as well as material analysis in microbiology genetic engineering and medicine. Aseptic culture of cells or tissues is designed to prevent external sources of contamination. Since the work is important, the present invention can be usefully used for this purpose. In particular, modern medicine is doing a variety of treatments using stem cells, which can be useful for culturing stem cells.

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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Abstract

La présente invention concerne un récipient pour cultiver des microorganismes ou des cellules dont une partie ou la totalité est constituée d'une membrane élastique et/ou d'une membrane filtrante comprenant du caoutchouc, du silicone, ou un matériau équivalent. Le récipient de culture comprend un corps ayant un trou traversant sur une surface et une membrane élastique à base d'élastomère pour interrompre le trou traversant du corps, la membrane élastique pouvant être une couche double. Une couche de stérilisation peut être incorporée, ou un filtre capable de ventiler l'air peut être ajouté.
PCT/KR2011/000440 2010-02-05 2011-01-21 Récipient de culture de microorganismes ou de cellules WO2011096659A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100010723A KR20110091078A (ko) 2010-02-05 2010-02-05 미생물의 오염을 방지하는 줄기세포 배양용기
KR10-2010-0010723 2010-02-05

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WO2011096659A2 true WO2011096659A2 (fr) 2011-08-11
WO2011096659A3 WO2011096659A3 (fr) 2012-02-02

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CN106967603A (zh) * 2017-04-11 2017-07-21 尹康康 一种趋磁细菌取样培养仪
CN107027694A (zh) * 2016-10-19 2017-08-11 中国水产科学研究院长江水产研究所 一种用于外源诱导剂导入鳖卵的装置及方法
CN107090401A (zh) * 2017-06-09 2017-08-25 天津施特雷生物科技股份有限公司 一种干制一次性无菌微生物培养皿
CN110205240A (zh) * 2019-06-22 2019-09-06 南阳市中心医院 一种基于用于检验科检验微生物细菌的培养皿
CN110447471A (zh) * 2019-07-31 2019-11-15 江南大学 一种叶片接种植物内生菌实验用植物培养装置
WO2023080852A1 (fr) * 2021-11-02 2023-05-11 Aktas Ranan Guelhan Récipient polyvalent pour matériaux biologiques et procédés
WO2023015293A3 (fr) * 2021-08-06 2024-04-04 The Regents Of The University Of California Dispositifs pour faire croître des cultures de micro-organismes

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KR101412155B1 (ko) * 2011-09-27 2014-06-27 동국대학교 산학협력단 3차원 세포 배양 용구 및 이를 이용한 세포의 3차원 배양 방법
WO2013047974A1 (fr) * 2011-09-27 2013-04-04 서울대학교 산학협력단 Appareil de culture de cellules tridimensionnelle et procédé de culture tridimensionnelle de cellules l'utilisant
KR101437147B1 (ko) * 2012-06-18 2014-09-02 주식회사 싸이토젠 3차원 스페로이드 배양 및 모니터링 시스템
WO2015056986A1 (fr) * 2013-10-16 2015-04-23 메디칸(주) Appareil et procédé pour culture cellulaire continue
KR200490369Y1 (ko) * 2014-10-30 2019-11-06 메디칸(주) 이동의 생존력을 유지하는 연속 세포 배양장치
KR102062585B1 (ko) * 2018-02-28 2020-01-06 글라드아이 주식회사 세포배양키트

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020134175A1 (en) * 2001-02-27 2002-09-26 Mehra Ravinder C. Pipette sampling system
US20070031963A1 (en) * 2005-06-01 2007-02-08 Chang Jim Y Cell culture flasks, systems, and methods for automated processing
JP2007192739A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 反応容器
WO2009026931A2 (fr) * 2007-08-24 2009-03-05 Smart Biosystems Aps Plateforme de bioréacteurs à moyenne échelle pour une perfusion
JP2009525756A (ja) * 2006-02-07 2009-07-16 ウェファージェン， インコーポレイテッド 温度制御された培養プレート
WO2009141163A2 (fr) * 2008-05-23 2009-11-26 Greiner Bio - One Gmbh Bioréacteur et procédé pour culture de cellules et de tissus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020134175A1 (en) * 2001-02-27 2002-09-26 Mehra Ravinder C. Pipette sampling system
US20070031963A1 (en) * 2005-06-01 2007-02-08 Chang Jim Y Cell culture flasks, systems, and methods for automated processing
JP2007192739A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 反応容器
JP2009525756A (ja) * 2006-02-07 2009-07-16 ウェファージェン， インコーポレイテッド 温度制御された培養プレート
WO2009026931A2 (fr) * 2007-08-24 2009-03-05 Smart Biosystems Aps Plateforme de bioréacteurs à moyenne échelle pour une perfusion
WO2009141163A2 (fr) * 2008-05-23 2009-11-26 Greiner Bio - One Gmbh Bioréacteur et procédé pour culture de cellules et de tissus

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016533177A (ja) * 2013-10-16 2016-10-27 メディカン インコーポレーテッド 連続的に細胞を培養する装置および方法
EP3059301A4 (fr) * 2013-10-16 2017-08-30 Medican Inc. Appareil et procédé pour culture cellulaire continue
CN107027694A (zh) * 2016-10-19 2017-08-11 中国水产科学研究院长江水产研究所 一种用于外源诱导剂导入鳖卵的装置及方法
CN107027694B (zh) * 2016-10-19 2019-10-01 中国水产科学研究院长江水产研究所 一种用于外源诱导剂导入鳖卵的装置及方法
CN106967603A (zh) * 2017-04-11 2017-07-21 尹康康 一种趋磁细菌取样培养仪
CN106967603B (zh) * 2017-04-11 2019-12-10 苏州理合文科技有限公司 一种趋磁细菌取样培养仪
CN107090401A (zh) * 2017-06-09 2017-08-25 天津施特雷生物科技股份有限公司 一种干制一次性无菌微生物培养皿
CN110205240A (zh) * 2019-06-22 2019-09-06 南阳市中心医院 一种基于用于检验科检验微生物细菌的培养皿
CN110447471A (zh) * 2019-07-31 2019-11-15 江南大学 一种叶片接种植物内生菌实验用植物培养装置
WO2023015293A3 (fr) * 2021-08-06 2024-04-04 The Regents Of The University Of California Dispositifs pour faire croître des cultures de micro-organismes
WO2023080852A1 (fr) * 2021-11-02 2023-05-11 Aktas Ranan Guelhan Récipient polyvalent pour matériaux biologiques et procédés

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