WO2011023843A3 - Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa - Google Patents

Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa Download PDF

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Publication number
WO2011023843A3
WO2011023843A3 PCT/ES2010/070569 ES2010070569W WO2011023843A3 WO 2011023843 A3 WO2011023843 A3 WO 2011023843A3 ES 2010070569 W ES2010070569 W ES 2010070569W WO 2011023843 A3 WO2011023843 A3 WO 2011023843A3
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WIPO (PCT)
Prior art keywords
agarose
tissue
cells
production
tissue engineering
Prior art date
Application number
PCT/ES2010/070569
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English (en)
French (fr)
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WO2011023843A2 (es
Inventor
Miguel Alaminos Mingorance
José Ignacio MUÑOZ ÁVILA
Miguel GONZÁLEZ ANDRADES
Antonio MUÑOZ CAMPOS
Ingrid Johanna GARZÓN BELLO
Original Assignee
Servicio Andaluz De Salud
Universidad De Granada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from ES200930625A external-priority patent/ES2353990B1/es
Priority claimed from ES200930943A external-priority patent/ES2362139B1/es
Application filed by Servicio Andaluz De Salud, Universidad De Granada filed Critical Servicio Andaluz De Salud
Priority to JP2012526090A priority Critical patent/JP6010460B2/ja
Priority to DK10811319.2T priority patent/DK2471902T3/en
Priority to US13/392,445 priority patent/US20120269776A1/en
Priority to ES10811319.2T priority patent/ES2677945T3/es
Priority to EP10811319.2A priority patent/EP2471902B9/en
Publication of WO2011023843A2 publication Critical patent/WO2011023843A2/es
Publication of WO2011023843A3 publication Critical patent/WO2011023843A3/es
Priority to US16/297,070 priority patent/US20190247541A1/en

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    • A—HUMAN NECESSITIES
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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Abstract

La presente invención proporciona un método in vitro de preparación de un tejido artificial, el tejido artificial obtenible por dicho método, la composición farmacéutica que lo comprende y el uso de este tejido o de esta composición para incrementar, restaurar o sustituir parcial o totalmente la actividad funcional de un tejido o un órgano dañado, preferiblemente piel, vejiga, uretra, córnea, mucosa oral, conjuntiva, pared abdominal, tímpano, faringe, laringe, intestino, peritoneo, ligamento, tendón, hueso, meninge o vagina. El método de la invención comprende los pasos de añadir fibrinógeno a células aisladas, preferiblemente, a fibroblastos o queratocitos, añadir un agente antifibrinolítico a la mezcla, añadir además un factor de coagulación, una fuente de calcio y trombina, añadir un polisacárido, preferiblemente agarosa, cultivar células, preferiblemente células epiteliales o células madre del cordón umbilical, en o sobre el producto resultante e inducir la nanoestructuracion de dicho producto. El método contempla también un paso intermedio que consiste en la adición de fibronectina tras el agente fibrinolítico, y otro paso en el que se añade colágeno después de la agarosa.
PCT/ES2010/070569 2009-08-25 2010-08-25 Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa WO2011023843A2 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2012526090A JP6010460B2 (ja) 2009-08-25 2010-08-25 フィブリンおよびアガロース生体材料を用いる組織工学による、人工組織の製造
DK10811319.2T DK2471902T3 (en) 2009-08-25 2010-08-25 Preparation of artificial tissues by tissue engineering with agarose-fibrin biomaterials
US13/392,445 US20120269776A1 (en) 2009-08-25 2010-08-25 Productions of artificial tissues by means of tissue engineering using agarose-fibrin biomaterials
ES10811319.2T ES2677945T3 (es) 2009-08-25 2010-08-25 Producción de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa
EP10811319.2A EP2471902B9 (en) 2009-08-25 2010-08-25 Production of artificial tissues by means of tissue engineering using agarose-fibrin biomaterials
US16/297,070 US20190247541A1 (en) 2009-08-25 2019-03-08 Preparation of artificial tissues by means of tissue engineering using fibrin and agarose biomaterials

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ES200930625A ES2353990B1 (es) 2009-08-25 2009-08-25 Elaboracion de tejidos artificiales mediante ingenieria tisular utilizando biomateriales de fibrina y agarosa.
ESP200930625 2009-08-25
ES200930943A ES2362139B1 (es) 2009-11-02 2009-11-02 Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina, agarosa y colágeno.
ESP200930943 2009-11-02

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/392,445 A-371-Of-International US20120269776A1 (en) 2009-08-25 2010-08-25 Productions of artificial tissues by means of tissue engineering using agarose-fibrin biomaterials
US16/297,070 Continuation US20190247541A1 (en) 2009-08-25 2019-03-08 Preparation of artificial tissues by means of tissue engineering using fibrin and agarose biomaterials

Publications (2)

Publication Number Publication Date
WO2011023843A2 WO2011023843A2 (es) 2011-03-03
WO2011023843A3 true WO2011023843A3 (es) 2011-07-07

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US (2) US20120269776A1 (es)
EP (1) EP2471902B9 (es)
JP (2) JP6010460B2 (es)
DK (1) DK2471902T3 (es)
ES (1) ES2677945T3 (es)
PT (1) PT2471902T (es)
TR (1) TR201809713T4 (es)
WO (1) WO2011023843A2 (es)

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EP2594295A1 (en) * 2011-11-16 2013-05-22 Servicio Andaluz De Salud Nerve implants based on a compacted biomaterial containing cells
FR2990106B1 (fr) * 2012-05-03 2014-05-09 Genoskin Systeme permettant la maintenance en survie et le transport de biospsies de peau et ses applications
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CL2013003066A1 (es) * 2013-10-22 2014-07-25 Univ Chile Composicion para tratamiento de heridas porque comprende matriz soporte y células troncalesmesenquiámicas de gelatina de wharton; metodo para tratar heridas que comprende aplicar dicha composicion
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