WO2011016485A1 - PROCÉDÉ D'INDUCTION D'UNE DIFFÉRENCIATION DE CELLULES iPS EN CELLULES PARENCHYMATEUSES HÉPATIQUES - Google Patents

PROCÉDÉ D'INDUCTION D'UNE DIFFÉRENCIATION DE CELLULES iPS EN CELLULES PARENCHYMATEUSES HÉPATIQUES Download PDF

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WO2011016485A1
WO2011016485A1 PCT/JP2010/063185 JP2010063185W WO2011016485A1 WO 2011016485 A1 WO2011016485 A1 WO 2011016485A1 JP 2010063185 W JP2010063185 W JP 2010063185W WO 2011016485 A1 WO2011016485 A1 WO 2011016485A1
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cells
ips
differentiation
hepatocytes
cell
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PCT/JP2010/063185
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English (en)
Japanese (ja)
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直哉 小林
和秀 山本
雅也 岩室
克身 持立
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国立大学法人岡山大学
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Priority to JP2011525912A priority Critical patent/JPWO2011016485A1/ja
Publication of WO2011016485A1 publication Critical patent/WO2011016485A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention porte sur un procédé pour induire une différenciation de cellules iPS en cellules parenchymateuses hépatiques. Ledit procédé fournit des cellules parenchymateuses hépatiques sans danger, suffisamment fonctionnelles, et peut produire de grandes quantités. Le procédé fourni comprend : (a) une étape dans laquelle des cellules iPS sont cultivées dans une culture en suspension et des corps embryoïdes sont formés; (b) une étape dans laquelle 10 à 15 corps embryoïdes par puits sont amenés à adhérer à une plaque recouverte de gélatine et cultivés, induisant des cellules d'endoderme embryonnaires; et (c) une étape dans laquelle HFG et DMSO sont ajoutés et les cellules d'endoderme embryonnaires sont cultivées sur un substrat de membrane basale, induisant une différenciation en cellules parenchymateuses hépatiques.
PCT/JP2010/063185 2009-08-04 2010-08-04 PROCÉDÉ D'INDUCTION D'UNE DIFFÉRENCIATION DE CELLULES iPS EN CELLULES PARENCHYMATEUSES HÉPATIQUES WO2011016485A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2011525912A JPWO2011016485A1 (ja) 2009-08-04 2010-08-04 ｉＰＳ細胞から肝実質細胞への分化誘導方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009-181891 2009-08-04
JP2009181891 2009-08-04

Publications (1)

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WO2011016485A1 true WO2011016485A1 (fr) 2011-02-10

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PCT/JP2010/063185 WO2011016485A1 (fr) 2009-08-04 2010-08-04 PROCÉDÉ D'INDUCTION D'UNE DIFFÉRENCIATION DE CELLULES iPS EN CELLULES PARENCHYMATEUSES HÉPATIQUES

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JP (1) JPWO2011016485A1 (fr)
WO (1) WO2011016485A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015523063A (ja) * 2012-05-23 2015-08-13 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 内胚葉細胞および肝実質細胞の組成物ならびにそれらの細胞を入手および使用する方法
WO2019131938A1 (fr) * 2017-12-27 2019-07-04 国立研究開発法人国立成育医療研究センター Procédé de préparation de cellules progénitrices hépatiques fonctionnelles ou de cellules hépatiques, ou de préparation de cellules progénitrices épithéliales de l'intestin grele fonctionnelles ou de cellules épithéliales de l'intestin grele
EP3431586A4 (fr) * 2016-02-29 2019-10-23 Yoshikazu Yonemitsu Cellules hépatiques hautement fonctionnelles et utilisation correspondante
CN114381420A (zh) * 2020-10-19 2022-04-22 清华大学 类肝组织结构体及其制备方法与应用
CN114377209A (zh) * 2020-10-19 2022-04-22 清华大学 含有胆管和肝脏组织的人工肝结构体及其制备方法与应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003093050A (ja) * 2001-09-25 2003-04-02 Japan Science & Technology Corp 基底膜の調製方法
JP2003093053A (ja) * 2001-09-25 2003-04-02 Japan Science & Technology Corp 基底膜標品の作製方法
WO2008066199A1 (fr) * 2006-12-01 2008-06-05 Naoya Kobayashi Procédé d'induction de la différenciation de cellules souches embryonnaires en cellules sécrétrices d'insuline, cellules sécrétrices d'insuline induites par le procédé et leur utilisation
JP2008306987A (ja) * 2007-06-15 2008-12-25 Covalent Materials Corp 細胞培養担体および細胞の培養方法
WO2009057831A1 (fr) * 2007-10-31 2009-05-07 Kyoto University Procédé de re-programmation nucléaire

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003093050A (ja) * 2001-09-25 2003-04-02 Japan Science & Technology Corp 基底膜の調製方法
JP2003093053A (ja) * 2001-09-25 2003-04-02 Japan Science & Technology Corp 基底膜標品の作製方法
WO2008066199A1 (fr) * 2006-12-01 2008-06-05 Naoya Kobayashi Procédé d'induction de la différenciation de cellules souches embryonnaires en cellules sécrétrices d'insuline, cellules sécrétrices d'insuline induites par le procédé et leur utilisation
JP2008306987A (ja) * 2007-06-15 2008-12-25 Covalent Materials Corp 細胞培養担体および細胞の培養方法
WO2009057831A1 (fr) * 2007-10-31 2009-05-07 Kyoto University Procédé de re-programmation nucléaire

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BASMA, H. ET AL.: "Differentiation and Transplantation of Human Embryonic Stem Cell- Derives Hepatocytes.", GASTROENTEROLOGY, vol. 163, no. 3, 29 October 2008 (2008-10-29), pages 990 - 999 *
HOSOKAWA, T. ET AL.: "Differentiation of tracheal basal cells to ciliated cells and tissue reconstruction on the synthesized basement membrane substratum in vitro.", CONNECT TISSUE RES., vol. 48, no. 1, 2007, pages 9 - 18 *
IWAMURO, M. ET AL.: "Comparative analysis of endoderm formation efficiency between mouse ES cells and iPS cells.", CELL TRANSPLANT., vol. 19, no. 6, 29 June 2010 (2010-06-29), pages 831 - 839, XP055117434, DOI: doi:10.3727/096368910X508951 *
IWAMURO, M. ET AL.: "Hepatic differentiation of mouse iPS cells in vitro.", CELL TRANSPLANT., vol. 19, no. 6, 29 June 2010 (2010-06-29), pages 841 - 847 *
NAOYA KOBAYASHI ET AL.: "Regeneration medicine of the liver", CLINIC ALL-ROUND, vol. 58, no. 1, January 2009 (2009-01-01), pages 79 - 85 *
SOTO-GUTIERREZ, A. ET AL.: "Reversal of mouse hepatic failure using an implanted liver- assist device containing ES cell-derived hepatocytes.", NAT. BIOTECHNOL., vol. 24, no. 11, 5 November 2006 (2006-11-05), pages 1412 - 1419, XP008127326, DOI: doi:10.1038/nbt1257 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015523063A (ja) * 2012-05-23 2015-08-13 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 内胚葉細胞および肝実質細胞の組成物ならびにそれらの細胞を入手および使用する方法
JP2017113005A (ja) * 2012-05-23 2017-06-29 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 内胚葉細胞および肝実質細胞の組成物ならびにそれらの細胞を入手および使用する方法
EP3431586A4 (fr) * 2016-02-29 2019-10-23 Yoshikazu Yonemitsu Cellules hépatiques hautement fonctionnelles et utilisation correspondante
WO2019131938A1 (fr) * 2017-12-27 2019-07-04 国立研究開発法人国立成育医療研究センター Procédé de préparation de cellules progénitrices hépatiques fonctionnelles ou de cellules hépatiques, ou de préparation de cellules progénitrices épithéliales de l'intestin grele fonctionnelles ou de cellules épithéliales de l'intestin grele
US20200339955A1 (en) * 2017-12-27 2020-10-29 Akihiro Umezawa Method for preparing functional hepatic progenitor cells or hepatic cells, or functional small intestinal epithelial progenitor cells or small intestinal epithelial cells
JPWO2019131938A1 (ja) * 2017-12-27 2021-08-26 明弘 梅澤 機能的肝前駆細胞もしくは肝細胞または機能的小腸上皮前駆細胞もしくは小腸上皮細胞を調製する方法
JP7191041B2 (ja) 2017-12-27 2022-12-16 明弘 梅澤 機能的肝前駆細胞もしくは肝細胞または機能的小腸上皮前駆細胞もしくは小腸上皮細胞を調製する方法
CN114381420A (zh) * 2020-10-19 2022-04-22 清华大学 类肝组织结构体及其制备方法与应用
CN114377209A (zh) * 2020-10-19 2022-04-22 清华大学 含有胆管和肝脏组织的人工肝结构体及其制备方法与应用
CN114377209B (zh) * 2020-10-19 2022-09-23 清华大学 含有胆管和肝脏组织的人工肝结构体及其制备方法与应用
CN114381420B (zh) * 2020-10-19 2024-01-30 清华大学 类肝组织结构体及其制备方法与应用

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