WO2011013806A1 - Procédé de production de cellules de schwann progénitrices, et procédé de prolifération desdites cellules de schwann progénitrices - Google Patents

Procédé de production de cellules de schwann progénitrices, et procédé de prolifération desdites cellules de schwann progénitrices Download PDF

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Publication number
WO2011013806A1
WO2011013806A1 PCT/JP2010/062915 JP2010062915W WO2011013806A1 WO 2011013806 A1 WO2011013806 A1 WO 2011013806A1 JP 2010062915 W JP2010062915 W JP 2010062915W WO 2011013806 A1 WO2011013806 A1 WO 2011013806A1
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Prior art keywords
cells
cell
transplant composition
schwann
isolated
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PCT/JP2010/062915
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English (en)
Japanese (ja)
Inventor
栄之 岡野
芳昭 戸山
雅也 中村
賢 石井
岳彦 高木
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学校法人慶應義塾
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Priority to JP2011524857A priority Critical patent/JP5794694B2/ja
Publication of WO2011013806A1 publication Critical patent/WO2011013806A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3878—Nerve tissue, brain, spinal cord, nerves, dura mater
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/383—Nerve cells, e.g. dendritic cells, Schwann cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Botany (AREA)
  • Dermatology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Transplantation (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Ophthalmology & Optometry (AREA)
  • Vascular Medicine (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un procédé de prolifération de cellules de Schwann progénitrices et un procédé de production de cellules de Schwann progénitrices. Quand des nerfs périphériques sont lésés, des cellules qui sont dérivées desdits nerfs périphériques et qui ont été isolées 24 heures à 6 semaines après la lésion, sont soumises à une culture flottante. Des cellules de Schwann progénitrices indifférenciées ayant une capacité de prolifération élevée peuvent ainsi être produites. Une composition de transplantation contenant lesdites cellules de Schwann progénitrices indifférenciées est efficace pour traiter les maladies associées à une lésion nerveuse ou à une neuropathie.
PCT/JP2010/062915 2009-07-30 2010-07-30 Procédé de production de cellules de schwann progénitrices, et procédé de prolifération desdites cellules de schwann progénitrices WO2011013806A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2011524857A JP5794694B2 (ja) 2009-07-30 2010-07-30 シュワン前駆細胞の製造方法及び増殖方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009-178340 2009-07-30
JP2009178340 2009-07-30

Publications (1)

Publication Number Publication Date
WO2011013806A1 true WO2011013806A1 (fr) 2011-02-03

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ID=43529457

Family Applications (1)

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PCT/JP2010/062915 WO2011013806A1 (fr) 2009-07-30 2010-07-30 Procédé de production de cellules de schwann progénitrices, et procédé de prolifération desdites cellules de schwann progénitrices

Country Status (2)

Country Link
JP (1) JP5794694B2 (fr)
WO (1) WO2011013806A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106687582A (zh) * 2014-08-04 2017-05-17 堪萨斯大学 哺乳动物多能干细胞、其产生方法及其用途
JP7214081B2 (ja) 2014-09-12 2023-01-30 ザ アドミニストレイターズ オブ ザ テューレイン エデュケイショナル ファンド 神経ミクロ生理学的システム及びこれを使用する方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000083655A (ja) * 1998-09-07 2000-03-28 Hitoshi Hirata シュワン細胞の純粋培養方法
JP2003070465A (ja) * 2001-08-31 2003-03-11 Keio Gijuku シュワン細胞の培養方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000083655A (ja) * 1998-09-07 2000-03-28 Hitoshi Hirata シュワン細胞の純粋培養方法
JP2003070465A (ja) * 2001-08-31 2003-03-11 Keio Gijuku シュワン細胞の培養方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HIROSHI HAMAOKA ET AL.: "Experimental Studies on the Purification of Schwann Cells by Using Double Soft Agar Culture", JOURNAL OF THE JUZEN MEDICAL SOCIETY, vol. 97, no. 4, 1988, KANAZAWA UNIVERSITY, pages 869 - 882 *
KEILHOFF G. ET AL.: "In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits.", J.NEUROSCI.METHODS, vol. 89, no. 1, 1999, pages 17 - 24 *
MITSURU IKEDA ET AL.: "A Novel Culture Method of Schwann Cell with the Serum-free Medium for Tissue-Engineered Nerve", PERIPHERAL NERVE, vol. 13, no. 1, 2002, pages 75 - 78 *
RODRIGUEZ F.J. ET AL.: "Nerve guides seeded with autologous schwann cells improve nerve regeneration.", EXP.NEUROL., vol. 161, no. 2, 2000, pages 571 - 584 *
TAKEHIKO TAKAGI ET AL.: "Sonsho Massho Shinkei Yurai no Mibunka Saibokai no Kaiseki", DAI 24 KAI ANNUAL RESEARCH MEETING OF THE JAPANESE ORTHOPAEDIC ASSOCIATION, ABSTRACTS, 25 August 2009 (2009-08-25), pages S1072 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106687582A (zh) * 2014-08-04 2017-05-17 堪萨斯大学 哺乳动物多能干细胞、其产生方法及其用途
JP2017529066A (ja) * 2014-08-04 2017-10-05 ユニバーシティ・オブ・カンザス 哺乳動物多能性幹細胞、それらの調製のための方法、およびその使用
EP3194573A4 (fr) * 2014-08-04 2018-01-17 University Of Kansas Cellules souches pluripotentes de mammifère, des procédés pour leur production et leurs utilisations
US10787658B2 (en) 2014-08-04 2020-09-29 University Of Kansas Mammalian pluripotent stem cells, methods for their production, and uses thereof
JP7214081B2 (ja) 2014-09-12 2023-01-30 ザ アドミニストレイターズ オブ ザ テューレイン エデュケイショナル ファンド 神経ミクロ生理学的システム及びこれを使用する方法

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JP5794694B2 (ja) 2015-10-14
JPWO2011013806A1 (ja) 2013-01-10

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