Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
  • G01D11/00—Component parts of measuring arrangements not specially adapted for a specific variable
  • G01D11/30—Supports specially adapted for an instrument; Supports specially adapted for a set of instruments
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
• • C—CHEMISTRY; METALLURGY
  • C21—METALLURGY OF IRON
  • C21C—PROCESSING OF PIG-IRON, e.g. REFINING, MANUFACTURE OF WROUGHT-IRON OR STEEL; TREATMENT IN MOLTEN STATE OF FERROUS ALLOYS
  • C21C5/00—Manufacture of carbon-steel, e.g. plain mild steel, medium carbon steel or cast steel or stainless steel
  • C21C5/52—Manufacture of steel in electric furnaces
  • C21C5/527—Charging of the electric furnace
• • C—CHEMISTRY; METALLURGY
  • C21—METALLURGY OF IRON
  • C21C—PROCESSING OF PIG-IRON, e.g. REFINING, MANUFACTURE OF WROUGHT-IRON OR STEEL; TREATMENT IN MOLTEN STATE OF FERROUS ALLOYS
  • C21C5/00—Manufacture of carbon-steel, e.g. plain mild steel, medium carbon steel or cast steel or stainless steel
  • C21C5/56—Manufacture of steel by other methods
  • C21C5/562—Manufacture of steel by other methods starting from scrap
  • C21C5/565—Preheating of scrap
• • C—CHEMISTRY; METALLURGY
  • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
  • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
  • C22B1/00—Preliminary treatment of ores or scrap
  • C22B1/005—Preliminary treatment of scrap
• • C—CHEMISTRY; METALLURGY
  • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
  • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
  • C22B19/00—Obtaining zinc or zinc oxide
  • C22B19/30—Obtaining zinc or zinc oxide from metallic residues or scraps
• • C—CHEMISTRY; METALLURGY
  • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
  • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
  • C22B7/00—Working up raw materials other than ores, e.g. scrap, to produce non-ferrous metals and compounds thereof; Methods of a general interest or applied to the winning of more than two metals
  • C22B7/006—Wet processes
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
  • Y02P10/00—Technologies related to metal processing
  • Y02P10/20—Recycling

Definitions

• the invention relates to a container having a sensor adapter for receiving a sensor arrangement for measuring at least one parameter of media contained in a container interior, wherein the sensor adapter is arranged on the wall to the container interior and an externally accessible receiving opening of a bounded towards the container interior receiving channel for the adaptation of Having sensor arrangement.
• a bioreactor or container which is in particular designed as a disposable mixing bag and a sensor adapter for receiving a sensor assembly in the form of an optical sensor, which is formed by one end of a light guide and is reversibly mounted has.
• the arranged in the wall to the reactor interior sensor adapter has an externally accessible receiving opening of a reactor to the interior of a transparent material bounded receiving channel.
• a disadvantage of the known sensor adapter is that it is only suitable for optical measuring operations. For installation of classical reversible electrodes or sensor arrangements for measuring the pH, the content of carbon dioxide or oxygen or for measuring the conductivity of the sensor adapter is not suitable. Also, the known adapters are not suitable for the use of gas sensors, as they are known for dissolved oxygen or dissolved carbon dioxide.
• sensor adapters are known from DE 10 2006 022 307 A1 and from WO 2006/017951 A1, which likewise have the abovementioned disadvantages.
• the known adapter must either use built-in sensors that are not reversible on the one hand and on the other hand are vulnerable to a necessary sterilization or the adapter must be open to the reactor interior. As far as the adapters are opened to the reactor interior, although replaceable gas sensors can be used, but they must then also be sterilized, which is associated with problems.
• containers are in particular containers for mixing, storing and transporting, as well as bioreactors and fermenters.
• the containers may be formed in particular from plastic.
• the media used are in particular liquids, gases, suspensions, dispersions, buffers and cell culture broths.
• parameters are understood to mean in particular concentration of substances, pressure and partial pressure of gases (oxygen, carbon dioxide), moisture, number of particles, turbidity, temperature, pH, electromagnetic radiation, fluorescence, electrical conductivity, as well as capacitive and electrical resistances.
• Object of the present invention is therefore to further develop the known container with a sensor adapter such that sensor arrangements can be used that need contact with the media to be measured, such as gas sensors.
• the interface to the reactor interior By the formation of at least part of the interface to the reactor interior as a membrane, it is possible that the medium to be measured with the sensors of the sensor array comes into direct contact. At the same time, it is avoided by the membrane that the interior of the reactor is contaminated by the sensor arrangement or its sensors.
• the sensor array with sensitive sensors need not be sterilized but only the sensor adapter attached to the bioreactor.
• the membranes have a pore size between 0.1 and 0.4 .mu.m, preferably of ⁇ 0.2 .mu.m.
• the membranes form a sterile barrier between the reactor interior and the reversibly attachable sensor arrangement.
• the membrane has hydrophilic, hydrophobic or oleophobic properties depending on the contacted medium.
• the first membrane can submerge in the liquid medium arranged in the reactor interior, while, for example, the second membrane is arranged in the head space of the reactor interior and has contact with the gas phase.
• the membrane has one or more regions with hydrophilic properties and one or more regions with hydrophobic properties.
• the membrane is formed as a hydrophilic membrane having a plurality of hydrophobic spots or as a hydrophobic membrane having a plurality of hydrophilic spots. So can at a feed of the Sensor assembly on the hydrophobic areas of the membrane gas are pressed sterile into the container interior. Liquid medium from the container interior can penetrate the membrane over the hydrophilic areas and wash around the sensor of the sensor arrangement.
• the membrane is made of a natural polymer, e.g. Cellulose acetate or regenerated cellulose formed.
• the membrane is formed of a synthetic polymer, such as polysulfone.
• the receiving channel forms a projecting into the container interior adapter shaft having two successively arranged membranes of different properties and the end face is closed.
• the receiving channel forms an adapter shaft projecting into the container interior, which has a first membrane closing its end face and a second membrane arranged on its circumference with different properties relative to the first membrane.
• the receiving channel may also form an adapter shaft projecting into the container interior, which has a first membrane arranged on its circumference, the end face of which is closed and having a side channel leading to the outside, the access opening lying outside of the reactor interior is also covered by a membrane or a membrane element having a filter element.
• the second membrane may also have hydrophilic, hydrophobic or other properties.
• the side channel leading to the outside is particularly suitable for obtaining cell-free liquid, which can be supplied to an analyzer.
• the sensor arrangement is piston-shaped in the receiving channel for generating a pressure arranged longitudinally displaceable.
• the longitudinal displaceability can be achieved, for example, by a bellows, via which the sensor arrangement arranged in the receiving channel is connected to the sensor adapter.
• the senor adapter is formed of a material resistant to beta radiation and / or gamma radiation and / or ethylene oxide (ETO).
• ETO ethylene oxide
• the sensor adapter can be arranged for example in a side wall or in the head region of the container. So its installation in height of the liquid located in the reactor interior is possible, but also an installation in the headspace.
• the membrane may be in contact with the liquid media, but may also be in contact with the gas phase in the headspace.
• the sensor adapter must be adapted to the intended sensor arrangement in each case.
• the container is designed as a disposable bioreactor.
• FIG. 1 shows a schematic side view of a container in an exploded view with a sensor adapter in section and an uncut sensor arrangement
• FIG. 2 shows a side view in section and elevation of a further container with a sensor adapter
• FIG. 3 shows a side view in section and elevation of a further container with a sensor adapter and outwardly leading side channel
• Figure 4 a side view in section and elevation of another container with a sensor adapter and a sensor assembly
• Figure 5 is a side view in section and elevation of another container with a sensor adapter, outwardly leading side channel and a membrane with different areas
• a container 2 which is formed in the embodiment as a disposable bioreactor 1, consists essentially of the container 2 and a sensor adapter.
• the container 2 is formed in the embodiment of Figure 1 as a foldable flexible bag, in the wall 4 of the sensor adapter 3 is arranged.
• the sensor adapter 3 has a flange 5, which is welded to the wall 4.
• the sensor adapter 3 has an adapter shaft 6, which projects from outside the container 2 through the wall 4 into the reactor interior 7.
• the sensor adapter 3 or its adapter shaft 6 has a receiving channel 8 centrally with a receiving opening 9 accessible from the outside. Via the receiving opening 9, a sensor arrangement for measuring a parameter of media contained in the reactor interior 7 into the receiving channel 8 can be inserted and reversibly connected to the sensor adapter 3.
• the adapter shaft 3 protrudes with its container-side end 11 into the reactor interior 7 inside.
• the container-side end 11 of the adapter shaft 6 is closed at its end face by a cover 12, which may be transparent.
• the adapter shaft 6 or the receiving channel 8 has a first diaphragm 13.
• the receiving channel 8 or the container-side end 11 of the adapter shaft 6 has a second membrane 14.
• the formed from a polymer membranes 13, 14 have a pore size of ⁇ 0.2 microns to form a sterile barrier between the reactor interior 7 and the receiving channel 8.
• the sensor arrangement 10 used in the receiving channel 8 has sensors 15, 16 assigned to the membranes 13, 14. At its end 17, which has the receiving opening 9, the receiving channel 8 or the adapter shaft 6 has a thread 18 for fastening the sensor arrangement 10.
• the wall of the receiving channel 8 has a sealing ring 18 for sealing and guiding the sensor assembly 10.
• the adapter shaft 6 'of the sensor adapter 3' at its the reactor interior 7 facing end side, the first membrane 13 'and adjacent to the periphery of the receiving channel 8, the second membrane 14' on.
• the adapter shaft 6 "of the sensor adapter 3" has the first diaphragm 13 "on its container-side end H". At its front end of the receiving channel 8 '' is closed by the cover 12.
• the adapter shaft 6 "has an outwardly leading side channel 19 from the receiving channel 8" on, which lies outside of the container interior access opening 20 of a second membrane 14 '' is covered.
• the membrane 13 '' '' has a region 22 with hydrophilic properties, which is interspersed by spots or regions 23 which form small islands and have hydrophobic properties.
• the adapter projection 25 is connected via a bellows 24 with the adapter shaft 6 '''', so that the sensor assembly 10 '''' in the receiving channel 8 '''' of the adapter shaft 6 '''' longitudinally displaceable.
• the adapter shaft 6 '''' has from the receiving channel 8 '''' an outwardly leading side channel 19 '''', the outside of the container interior I 1 '''lying access opening 20'''''of a second membrane 14'''' is covered.

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• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Organic Chemistry (AREA)
• Materials Engineering (AREA)
• Manufacturing & Machinery (AREA)
• Metallurgy (AREA)
• Mechanical Engineering (AREA)
• Life Sciences & Earth Sciences (AREA)
• Geology (AREA)
• General Life Sciences & Earth Sciences (AREA)
• Environmental & Geological Engineering (AREA)
• General Physics & Mathematics (AREA)
• Physics & Mathematics (AREA)
• Geochemistry & Mineralogy (AREA)
• Wood Science & Technology (AREA)
• Zoology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Health & Medical Sciences (AREA)
• Biotechnology (AREA)
• Genetics & Genomics (AREA)
• General Health & Medical Sciences (AREA)
• General Engineering & Computer Science (AREA)
• Biochemistry (AREA)
• Sustainable Development (AREA)
• Microbiology (AREA)
• Biomedical Technology (AREA)
• Analytical Chemistry (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Sampling And Sample Adjustment (AREA)
• Separation Using Semi-Permeable Membranes (AREA)

Priority Applications (3)

Applications Claiming Priority (4)

Publications (2)

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• 2009
  • 2009-08-14 DE DE200910037345 patent/DE102009037345A1/de not_active Withdrawn
• 2010
  • 2010-05-07 JP JP2012515367A patent/JP5781505B2/ja active Active
  • 2010-05-07 EP EP10739293.8A patent/EP2443420B1/de active Active
  • 2010-05-07 WO PCT/EP2010/002808 patent/WO2010145735A2/de not_active Ceased
  • 2010-05-07 US US13/376,454 patent/US9103703B2/en active Active

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