WO2010095677A1 - Plaque cible et procédé de production associé - Google Patents

Plaque cible et procédé de production associé Download PDF

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Publication number
WO2010095677A1
WO2010095677A1 PCT/JP2010/052424 JP2010052424W WO2010095677A1 WO 2010095677 A1 WO2010095677 A1 WO 2010095677A1 JP 2010052424 W JP2010052424 W JP 2010052424W WO 2010095677 A1 WO2010095677 A1 WO 2010095677A1
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WO
WIPO (PCT)
Prior art keywords
target plate
sample
lid
contact
holder
Prior art date
Application number
PCT/JP2010/052424
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English (en)
Japanese (ja)
Inventor
渉 服部
Original Assignee
日本電気株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日本電気株式会社 filed Critical 日本電気株式会社
Publication of WO2010095677A1 publication Critical patent/WO2010095677A1/fr

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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Definitions

  • the present invention relates to a target plate used for a matrix-assisted laser desorption / ionization mass spectrometer (MALDI-MS) for fixing a sample mounting substrate and a manufacturing method thereof.
  • MALDI-MS matrix-assisted laser desorption / ionization mass spectrometer
  • MALDI-TOF-MS matrix-assisted laser desorption ionization time-of-flight mass spectrometer
  • imaging MS a mass spectrometry imaging technique called imaging MS
  • a tissue section is irradiated with a laser, and mass spectrometry is performed for each irradiation position.
  • the sample separated in the flow path provided on the chip is irradiated with a laser according to the separated position, and mass spectrometry is performed for each irradiation position.
  • a sample such as a biological tissue used in such an analysis has a thickness, and it is not easy to obtain the conductivity of the sample even if it is placed on a conductive substrate.
  • a sample to be analyzed may be placed on an insulating substrate such as placed on a slide glass or fixed with paraffin or the like.
  • separation techniques such as capillary electrophoresis and electrophoresis chromatography are often used for the chip, and the chip is often an insulator. Therefore, it is important to perform mass spectrometry imaging using MALDI-TOF-MS on an insulating substrate.
  • the filler contained in the applied matrix can be made conductive, and can be electrically connected to the target plate on which the slide glass or the chip is placed, thereby preventing charging.
  • this method has a problem that the metal filler reflects the laser and suppresses ionization of the sample.
  • the laser absorbs a laser and generates heat, partially decomposes the sample and lowers the detection sensitivity of the sample.
  • an ionic liquid having conductivity may be used as a matrix.
  • the liquid matrix is in a liquid state even at room temperature, the position and amount on the sample surface are not stable, such as flowing on the sample surface. Further, coupled with fluidity, for example, the pattern of the sample separated on the chip may be disturbed. For this reason, when using an ionic liquid as a matrix, there existed a problem of reducing position resolution.
  • the thickness of the conductive film is approximately 100 nm or less, the laser transmits to some extent, so that mass spectrometry can be performed.
  • the laser intensity decreases.
  • the number of samples to be ionized decreases and the detection sensitivity decreases.
  • a method for solving this problem a method called atmospheric pressure MALDI-TOF-MS has been devised.
  • a sample is first placed in the atmosphere, and a sample ionized by laser irradiation is sucked into the vacuum together with the atmosphere. After that, acceleration is performed by applying a voltage, and the time of flight is measured for mass analysis. In this case, since a voltage is not directly applied to the insulator substrate, charging of the sample can be prevented.
  • the sample since the sample is installed in the atmosphere, even if the sample is charged, the sample is neutralized by a large number of ions present in the atmosphere.
  • the sample in the method using the atmospheric pressure MALDI-TOF-MS, the sample is easily oxidized and deteriorated because the sample is in the atmosphere.
  • the flight path of the ionized sample since the flight path of the ionized sample is complicated, there are many sample losses and the sensitivity is poor.
  • the number of manufacturers that manufacture atmospheric pressure MALDI-TOF-MS is limited, there is a problem that the diffusion rate of the apparatus is extremely low.
  • the present invention has been made to solve the above-described problems, and it is an object of the present invention to enable accurate mass spectrometry even when an insulating substrate is used in MALDI-MS analysis.
  • a target plate according to the present invention is a target plate used in a matrix-assisted laser desorption / ionization mass spectrometer, a holder for holding a sample substrate on which a sample is placed, and an opening corresponding to a measurement target region of the sample substrate
  • the holder and the lid portion have conductivity at least on the surface, and the opening portion has a contact portion that comes into contact with the sample substrate.
  • the target plate manufacturing method is a target plate manufacturing method used in a matrix-assisted laser desorption / ionization mass spectrometer, and includes a step of forming a holder for holding a sample substrate on which a sample is placed. And a step of forming a lid, and a step of forming an opening corresponding to the measurement target region of the sample substrate of the lid.
  • the holder and the lid have conductivity at least on the surface, and the opening is And a contact portion that contacts the sample substrate.
  • FIG. 1 is a perspective view showing a configuration of a target plate in Embodiment 1 of the present invention.
  • FIG. 2 is a perspective view showing the configuration of the target plate in the second embodiment of the present invention.
  • FIG. 3 is a cross-sectional view showing the configuration of the target plate in the second embodiment of the present invention.
  • FIG. 4 is a cross-sectional view showing the configuration of the target plate in the third embodiment of the present invention.
  • FIG. 5 is a perspective view showing the configuration of the target plate in the fourth embodiment of the present invention.
  • FIG. 6 is a cross-sectional view showing the configuration of the target plate in the fifth embodiment of the present invention.
  • FIG. 1 is a perspective view showing a configuration of a target plate in Embodiment 1 of the present invention.
  • This target plate is used in a matrix-assisted laser desorption / ionization mass spectrometer (MALDI-MS), and includes a holder 102 that holds a sample substrate 111 on which a sample is placed, and a measurement target region 181 of the sample substrate 111. And a lid 101 in which an opening 108 corresponding to is formed.
  • MALDI-MS includes time-of-flight (TOF), quadrupole, and Fourier transform ion cyclotron resonance types.
  • the lid 101 and the holder 102 have conductivity at least on the surface.
  • the lid 101 and the holder 102 are made of a metal material such as an aluminum alloy or SUS403.
  • the lid 101 has a thickness of about 0.3 mm to 0.5 mm. Therefore, when the lid 101 is placed on the holder 102 on which the sample substrate 111 is placed and the contact portion 109 is brought into contact with the sample substrate 111, the distance between the upper surface of the lid 101 and the surface of the sample substrate 111 is The lid 101 has a thickness of about 0.3 to 0.5 mm.
  • the lid 101 and the holder 102 may be made of, for example, plastic, and a metal film may be formed on the surface.
  • the target plate in the present embodiment is characterized in that the opening 108 of the lid 101 has at least two linear contact portions 109 that extend to the measurement target region 181 and abut against the sample substrate 111.
  • FIG. 1 shows a case where the opening 108 is formed in a rectangular shape and includes two linear contact portions 109 arranged in parallel, and these contact portions 109 come into contact with the contact portion 191 of the sample substrate 111. Yes. Note that two contact portions 109 adjacent to the opening 108 may be in contact with the sample substrate 111. Further, the contact portions 109 on the four sides constituting the opening 108 may be in contact with the sample substrate 111.
  • a method for manufacturing the above-described target plate will be described.
  • a holder for holding a sample substrate on which a sample is placed is formed.
  • a cover part is formed.
  • an opening corresponding to the measurement target region of the sample substrate of the lid is formed.
  • the holder and the lid are formed with conductivity at least on the surface.
  • two linear contact portions that extend to at least the measurement target region and abut against the sample substrate are formed in the opening.
  • the two contact portions 109 come into contact with the sample substrate 111.
  • the two contact portions 109 are formed in a straight line and extend to the measurement target region 181. Accordingly, the contact portion 109 comes into contact with the surface of the sample substrate 111 so that a surface (plane) equipotential to the lid portion 101 is formed.
  • a plane equipotential to the lid 101 is formed over the entire measurement target region 181.
  • the lines of electric force generated by the applied voltage for extracting and accelerating the ions are generated in the sample region 181 in the sample substrate. Incidently enters the surface of 111.
  • the lines of electric force that should be perpendicularly incident on the sample substrate may be distorted by charging of the sample.
  • the surface having the same potential as the lid 101 is provided in contact with the surface of the sample substrate 111.
  • electric force lines which are properties of electric lines of force, have the continuity with the surrounding equipotential surfaces even on the sample substrate 111 made of an insulating material because of the property of being arranged in the most spatially separated manner. It tries to arrange it so that it is perpendicularly incident. Due to this property, an equipotential surface is also formed on the surface of the sample substrate 111.
  • the lines of electric force generated by the applied voltage for extracting and accelerating the ions are perpendicularly incident on the surface of the sample substrate 111 if an equipotential surface is formed.
  • the contact part may be either linear or curved.
  • the straight line can more prevent the sample from being charged.
  • the contact portion may be in contact with the sample substrate. Furthermore, it is preferable that the contact portion extends near or in the measurement target region of the sample substrate and is in contact with the sample substrate. The contact portion preferably extends, for example, to a distance within about 1.5 mm from the outer extension of the sample measurement target region.
  • the lines of electric force are incident vertically, according to the present embodiment, it is possible to use a normal matrix material that is dried and becomes a solid. Further, it is not necessary to mix a filler that causes a decrease in sensitivity into the matrix solution and to form a conductive film. Therefore, it is possible to accurately measure the mass of ions generated from the sample without causing problems such as a decrease in position resolution and a decrease in detection sensitivity caused by these matrices.
  • FIG. 2 is a perspective view showing the configuration of the target plate in the second embodiment of the present invention.
  • This target plate is used in a matrix-assisted laser desorption / ionization mass spectrometer, and has a holder 202 for holding a sample substrate (not shown) on which a sample is placed, and an opening corresponding to a measurement target region of the sample substrate. And a lid 201 on which a portion 208 is formed.
  • the holder 202 includes a guide 203, a positioning pin 204, a magnet 206, and a housing part 207.
  • the lid 201 includes a through hole 205 and a conductive rubber sheet 209.
  • a guide 203 is added to the configuration of the first embodiment described above, and a positioning pin 204 and a through hole 205 are added.
  • the lid 201 is fitted in the holder 202 in accordance with the guide 203.
  • a pair (one set) of positioning pins 204 are fitted into a pair (one set) of through holes 205 provided in the lid portion 201, and the relative position between the lid portion 201 and the holder 202 is determined. The Therefore, according to the second embodiment, it is easy to determine the relative positions of the holder 202, the sample substrate, and the lid 201.
  • the positioning pin 204 is provided on the holder 202 side and the corresponding through hole 205 is provided on the lid 201 side.
  • the present invention is not limited to this.
  • a positioning pin may be provided on the lid 201 and a hole corresponding to the holder 202 may be provided.
  • the magnet 206 is added to the configuration of the first embodiment described above.
  • the cover part 201 is comprised from the electroconductive material which has magnetism, such as SUS403, for example.
  • the lid 201 is attracted to a group of magnets 206 embedded in the holder 202 with the same polarity, and the lid 201 can be fixed to the holder 202.
  • attachment and detachment become easy by fixing with a magnet.
  • the magnet 206 embedded in the holder 202 has, for example, the upper side (the lid 201 side) aligned with the S pole. Further, the lid 201 that is attracted to the magnet 206 is used. By doing so, when the target plate is inserted into the MALDI-MS, the magnetic field is uniformly applied to the electromagnetic optical system located on the target plate, and adverse effects on the measurement can be suppressed.
  • the holder 202 is preferably made of a conductive material. Further, in order to make the polarity of the magnet 206 to be embedded uniform, it is desirable to make it from a non-magnetized material such as aluminum.
  • an accommodating portion 207 made of a recess into which the sample substrate is fitted is formed on the upper surface of the holder 202.
  • two accommodating portions 207 are provided on the upper surface of the holder 202.
  • a sample substrate on which a sample is placed is fitted in the accommodating portion 207, and the lid portion 201 is placed thereon.
  • the opening portion 208 is provided in the lid portion 201 in accordance with the position of the accommodating portion 207 so that a portion (measurement target region) of the sample to be measured is exposed from the target plate (lid portion 201).
  • a conductive rubber sheet 209 is provided on each side of the opening 208 formed in a rectangular shape.
  • the conductive rubber sheet 209 is used as the contact portion 109 of the first embodiment described above.
  • the conductive rubber sheet 209 is electrically connected to the lid portion 201.
  • the conductive rubber sheet 209 has elasticity with the sample substrate when covered with the lid portion 201 and uniformly contacts along the side of the opening 208, and electrically connects the lid portion 201 and the sample substrate. Connect. Therefore, according to the second embodiment, compared to the first embodiment described above, the lid 201 and the sample substrate can be contacted more uniformly as will be described later.
  • the conductive rubber sheet 209 is disposed on all four sides of the opening 208.
  • the lid portion 201 is attracted by the magnet 206, and the conductive rubber sheet 209 becomes the chip 201. Pressed against the surface.
  • FIG. 3 shows a cross section taken along line AA 'of FIG.
  • the surface of the insulating chip 201 is affected by the equipotential surface formed by the conductive rubber sheet 209.
  • the lines of electric force arranged in the are arranged so as to form an equipotential surface.
  • the conductive rubber sheet 209 and the surface of the chip 201 in contact with the conductive rubber sheet 209 are equipotential. As a result, charging of the sample can be prevented.
  • tip which can measure exact mass can be provided.
  • the conductive rubber sheet 209 is used, but the present invention is not limited to this.
  • the portion of the conductive rubber sheet 209 only needs to be made of an elastic body having conductivity.
  • a spring such as a coil spring or a leaf spring made of a conductive material such as metal may be used.
  • FIG. 4 is a cross-sectional view showing the configuration of the target plate in the third embodiment of the present invention.
  • This target plate is used in a matrix-assisted laser desorption / ionization mass spectrometer, and has a holder 402 for holding a slide glass 410 on which a sample is placed, and an opening 408 corresponding to a measurement target region of the slide glass 410.
  • a lid portion 401 formed with the.
  • the holder 402 includes a guide 403, a fitting recess 404, a screw hole 405, and a storage portion 407.
  • the lid portion 401 includes a fitting convex portion 411, a leaf spring portion 412, and a through hole 413.
  • the lid 401 can be fixed to the holder 402 by fitting the fitting convex portion 411 into the fitting concave portion 404 and screwing the screw 421 passing through the through hole 413 into the screw hole 405. Further, the slide glass 410 is pressed against the housing portion 407 and fixed by the leaf spring portion 412 of the lid portion 401 fixed to the holder 402 in this way.
  • the contact portion 109 in the first embodiment is configured by the leaf spring portion 412. Therefore, according to the third embodiment, the lid 201 and the sample substrate can be brought into contact with each other more reliably than in the first embodiment described above.
  • the cover part 401 is comprised from a metal plate with a plate thickness of about 300 ⁇ m
  • the leaf spring part 412 can be formed integrally with the cover part 401 by bending or cutting.
  • FIG. 5 is a perspective view showing the configuration of the target plate in the fourth embodiment of the present invention.
  • This target plate is used in a matrix-assisted laser desorption / ionization mass spectrometer, and has a holder 502 for holding a sample substrate (not shown) on which a sample is placed, and an opening corresponding to a measurement target region of the sample substrate.
  • two lid portions 501 formed with a portion 508.
  • the holder 502 includes a guide 503, a positioning pin 504, a magnet 506, and two accommodating portions 507.
  • the lid portion 501 includes a through hole 505 and a conductive rubber sheet 509.
  • Each lid 501 is fitted in the holder 502 in accordance with the guide 503.
  • a set of positioning pins 504 are fitted into a set of through holes 505 provided in the lid portion 501, and the relative positions of the lid portion 501 and the holder 502 are determined.
  • a positioning pin 504 is provided on the holder 502 side, and a corresponding through hole 505 is provided on the lid portion 501 side.
  • a positioning pin may be provided on the lid 501 and a hole corresponding to the holder 502 may be provided.
  • the lid portion 501 may be made of a conductive material having magnetism such as SUS403. By being made of a material having magnetism, the lid portion 501 is attracted to a group of magnets 506 embedded in the holder 502 with the same polarity, and the lid portion 501 can be fixed to the holder 502. Moreover, if it fixes with a magnet, attachment or detachment will become easy.
  • the magnet 506 embedded in the holder 202 has, for example, the upper side (the lid portion 501 side) aligned with the S pole. Further, a lid 501 that is attracted to the magnet 506 is used. By doing so, when the target plate is inserted into the MALDI-MS, the magnetic field is uniformly applied to the electromagnetic optical system located on the target plate, and adverse effects on the measurement can be suppressed.
  • the holder 502 is preferably made of a conductive material. Furthermore, in order to align the polarity of the magnet 506 to be embedded, it is desirable to make it from a non-magnetized material such as aluminum.
  • an accommodating portion 507 formed of a recess into which the sample substrate is fitted is formed on the upper surface of the holder 502.
  • two accommodating portions 507 are provided.
  • a plurality of lid portions 501 are provided in accordance with the number of accommodating portions 507.
  • a sample substrate on which a sample is placed is fitted in each of the two accommodating portions 507, and a lid portion 501 is placed on each sample substrate.
  • the lid portions 501 can be covered in accordance with the thicknesses of the respective sample substrates.
  • an opening 508 is provided in the lid portion 501 in accordance with the position of the accommodating portion 507 so that a portion (measurement target region) of the sample to be measured is exposed from the target plate (lid portion 501).
  • a conductive rubber sheet 509 is provided on the side of the opening 508.
  • the conductive rubber sheet 509 is provided in a state of being electrically connected to the lid portion 501.
  • the conductive rubber sheet 509 has elasticity with the sample substrate when the cover portion 501 is covered, and makes uniform contact along the side of the opening 508, and electrically connects the cover portion 501 and the sample substrate. Connect.
  • conductive rubber sheets 509 are provided on two opposite sides of the opening 508.
  • conductive rubber sheets 509 are provided on two sides along the wide direction of the holder 502 that is rectangular in plan view.
  • a chip 510 as a sample substrate is fitted in the housing portion 507 and a lid portion 501 is put on the housing portion 507.
  • the lid 501 is attracted by the magnet 506 and the conductive rubber sheet 509 is pressed against the surface of the chip 510.
  • a relief is formed in consideration of the protruding region of the conductive rubber sheet 509, and the stepping for positioning the substrate is performed. Can be done.
  • FIG. 6 shows a cross section taken along line BB ′ of FIG.
  • the surface of the insulating chip 510 is affected by the equipotential surface formed by the conductive rubber sheet 509.
  • the lines of electric force arranged in the are arranged so as to form an equipotential surface.
  • the conductive rubber sheet 509 and the surface of the chip 510 in contact with the conductive rubber sheet 509 are equipotential and are not charged.
  • a measure that tends to be equipotential is taken especially in the direction in which the lines of electric force tend to be distorted. Will be.
  • a lid without an opening 508 may be used in the accommodating portion 507 where the sample substrate is not disposed.
  • the recess of the accommodating portion 507 in which the sample substrate is accommodated is closed, and an equipotential surface with higher flatness is formed on the target plate.
  • the target plate in the present embodiment at least two sides of the surface of the sample substrate are in contact with at least a length along the side surrounding the region to be measured of the sample on the sample substrate. It has the structure to do.
  • the part in contact is a part of the lid part or a conductor (conductive rubber sheet 509) that is electrically connected to the lid part.
  • a target plate used in a matrix-assisted laser desorption / ionization mass spectrometer A holder for holding a sample substrate on which the sample is placed; A lid portion having an opening corresponding to the measurement target region of the sample substrate, The holder and the lid have conductivity on at least the surface, The opening is a target plate having a contact portion that comes into contact with the sample substrate.
  • the opening is a target plate having at least two contact portions that come into contact with the sample substrate.
  • the said contact part is a target plate arrange
  • the opening is a target plate that extends to the measurement target region and contacts the sample substrate.
  • the said contact part is a target plate comprised from the elastic body which has electroconductivity, and is electrically connected and arrange
  • the contact portion is a target plate disposed on the leaf spring portion.
  • the said cover part is a target plate provided with the said several opening part.
  • a target plate provided with a recess-shaped accommodation part for accommodating the sample substrate in the holder.
  • a target plate comprising a plurality of the accommodating portions and a plurality of the lid portions for each accommodating portion.
  • the lid corresponding to the accommodating portion that does not accommodate the sample substrate is a target plate in which the opening is not formed.
  • a target plate provided with a pin for determining the position of the lid with respect to the holder and a through hole corresponding to the pin in the holder and the lid.
  • a method for producing a target plate for use in a matrix-assisted laser desorption / ionization mass spectrometer comprising: Forming a holder for holding a sample substrate on which the sample is placed; Forming a lid, Forming an opening corresponding to the measurement target region of the sample substrate of the lid, and The holder and the lid have conductivity on at least the surface,
  • the said opening part is a manufacturing method of the target plate which has a contact part contact
  • the said opening part is a manufacturing method of the target plate which has at least 2 contact part contact
  • Appendix 15 In the method for manufacturing a target plate according to appendix 14, The method for manufacturing a target plate, wherein the at least two contact portions are linear.
  • a target plate that suppresses charging of a sample when a sample placed on an insulating sample substrate is analyzed by MALDI-MS.
  • the target plate of the present invention it is possible to suppress the deterioration of performance such as the sample itself, detection sensitivity and position resolution, and to measure an accurate mass.
  • a slide glass or a chip can be placed on the target plate.

Abstract

L'invention concerne une plaque cible composée d'un dispositif de maintien (102) qui maintient un substrat d'échantillon (111) sur lequel est placé un échantillon, et une fermeture (101) qui comporte une ouverture (108) correspondant à une région cible de mesure (181) du substrat d'échantillon (111). Au moins les surfaces de la fermeture (101) et du dispositif de maintien (102) sont électroconductrices et l'ouverture (108) comporte une portion de contact (109) en butée avec le substrat d'échantillon (111).
PCT/JP2010/052424 2009-02-18 2010-02-18 Plaque cible et procédé de production associé WO2010095677A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009034991 2009-02-18
JP2009-034991 2009-02-18

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WO2010095677A1 true WO2010095677A1 (fr) 2010-08-26

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014021048A (ja) * 2012-07-23 2014-02-03 Jeol Ltd サンプルプレートおよび質量分析装置
JP2017117719A (ja) * 2015-12-25 2017-06-29 国立大学法人秋田大学 試料ホルダーおよびその固定具
WO2021157169A1 (fr) * 2020-02-04 2021-08-12 浜松ホトニクス株式会社 Porte-échantillon et procédé de fabrication de porte-échantillon
CN113574374A (zh) * 2019-03-19 2021-10-29 浜松光子学株式会社 试样支承体、离子化方法和质量分析方法
CN113574372A (zh) * 2019-03-19 2021-10-29 浜松光子学株式会社 试样支承体、离子化方法和质量分析方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003510776A (ja) * 1999-09-27 2003-03-18 ルドウイッヒ インスティテュート フォア キャンサー リサーチ 液体ｍａｌｄｉｍｓで使用される改良イオン源ターゲット
JP2003534634A (ja) * 2000-05-23 2003-11-18 エピゲノミクス アーゲー 質量分析計の試料支持台
JP2007508552A (ja) * 2003-10-10 2007-04-05 プロテイン・デイスカバリー・インコーポレーテツド マトリックス支援レーザー脱離／イオン化（ｍａｌｄｉ）質量分析法（ｍｓ）を包含する化学分析のための被検体の濃縮および精製のための方法および装置
JP2007508546A (ja) * 2003-10-10 2007-04-05 アプレラ コーポレイション 取り外し可能な挿入物を備えるｍａｌｄｉプレート
JP2007514956A (ja) * 2003-12-19 2007-06-07 アプレラ コーポレイション 格子を備えるｍａｌｄｉプレート構築物

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003510776A (ja) * 1999-09-27 2003-03-18 ルドウイッヒ インスティテュート フォア キャンサー リサーチ 液体ｍａｌｄｉｍｓで使用される改良イオン源ターゲット
JP2003534634A (ja) * 2000-05-23 2003-11-18 エピゲノミクス アーゲー 質量分析計の試料支持台
JP2007508552A (ja) * 2003-10-10 2007-04-05 プロテイン・デイスカバリー・インコーポレーテツド マトリックス支援レーザー脱離／イオン化（ｍａｌｄｉ）質量分析法（ｍｓ）を包含する化学分析のための被検体の濃縮および精製のための方法および装置
JP2007508546A (ja) * 2003-10-10 2007-04-05 アプレラ コーポレイション 取り外し可能な挿入物を備えるｍａｌｄｉプレート
JP2007514956A (ja) * 2003-12-19 2007-06-07 アプレラ コーポレイション 格子を備えるｍａｌｄｉプレート構築物

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014021048A (ja) * 2012-07-23 2014-02-03 Jeol Ltd サンプルプレートおよび質量分析装置
JP2017117719A (ja) * 2015-12-25 2017-06-29 国立大学法人秋田大学 試料ホルダーおよびその固定具
CN113574374A (zh) * 2019-03-19 2021-10-29 浜松光子学株式会社 试样支承体、离子化方法和质量分析方法
CN113574372A (zh) * 2019-03-19 2021-10-29 浜松光子学株式会社 试样支承体、离子化方法和质量分析方法
WO2021157169A1 (fr) * 2020-02-04 2021-08-12 浜松ホトニクス株式会社 Porte-échantillon et procédé de fabrication de porte-échantillon
JP7333280B2 (ja) 2020-02-04 2023-08-24 浜松ホトニクス株式会社 試料支持体及び試料支持体の製造方法

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