WO2010075960A3 - Verfahren zur herstellung von riboflavin - Google Patents

Verfahren zur herstellung von riboflavin Download PDF

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Publication number
WO2010075960A3
WO2010075960A3 PCT/EP2009/008987 EP2009008987W WO2010075960A3 WO 2010075960 A3 WO2010075960 A3 WO 2010075960A3 EP 2009008987 W EP2009008987 W EP 2009008987W WO 2010075960 A3 WO2010075960 A3 WO 2010075960A3
Authority
WO
WIPO (PCT)
Prior art keywords
riboflavin
host cell
producing riboflavin
producing
riboflavin production
Prior art date
Application number
PCT/EP2009/008987
Other languages
English (en)
French (fr)
Other versions
WO2010075960A8 (de
WO2010075960A2 (de
Inventor
Florian Kirchner
Klaus Mauch
Joachim Schmid
Original Assignee
Dsm Ip Assets B.V.
In Silico Biotechnology Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V., In Silico Biotechnology Ag filed Critical Dsm Ip Assets B.V.
Priority to US13/139,896 priority Critical patent/US8759024B2/en
Priority to KR1020117016203A priority patent/KR101739147B1/ko
Priority to EP09796959A priority patent/EP2358893A2/de
Priority to JP2011539963A priority patent/JP5813512B2/ja
Priority to CN200980150515.7A priority patent/CN102245781B/zh
Publication of WO2010075960A2 publication Critical patent/WO2010075960A2/de
Publication of WO2010075960A3 publication Critical patent/WO2010075960A3/de
Publication of WO2010075960A8 publication Critical patent/WO2010075960A8/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft Verfahren und Mittel zur biotechnologisch fermentativen Herstellung von Riboflavin (im folgenden auch als Vitamin B2 bezeichnet) sowie Mittel zur Durchführung dieses Verfahrens, besonders eine modifizierte mikrobielle Wirtszelle mit gesteigerter Riboflavinausbeute. Die Erfindung stellt somit neue Verfahren und Mittel zur Regulation der Expression von an der Riboflavinproduktion der Wirtszelle beteiligten Enzymaktivitäten bereit.
PCT/EP2009/008987 2008-12-15 2009-12-15 Verfahren zur herstellung von riboflavin WO2010075960A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US13/139,896 US8759024B2 (en) 2008-12-15 2009-12-15 Process for the production of riboflavin
KR1020117016203A KR101739147B1 (ko) 2008-12-15 2009-12-15 리보플라빈의 제조 방법
EP09796959A EP2358893A2 (de) 2008-12-15 2009-12-15 Verfahren zur herstellung von riboflavin
JP2011539963A JP5813512B2 (ja) 2008-12-15 2009-12-15 リボフラビンの生産方法
CN200980150515.7A CN102245781B (zh) 2008-12-15 2009-12-15 用于生产核黄素的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008063234A DE102008063234B4 (de) 2008-12-15 2008-12-15 Biotechnologische Herstellung von Riboflavin mit hoher Ausbeute
DE102008063234.1 2008-12-15

Publications (3)

Publication Number Publication Date
WO2010075960A2 WO2010075960A2 (de) 2010-07-08
WO2010075960A3 true WO2010075960A3 (de) 2010-09-10
WO2010075960A8 WO2010075960A8 (de) 2011-06-23

Family

ID=42025765

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/008987 WO2010075960A2 (de) 2008-12-15 2009-12-15 Verfahren zur herstellung von riboflavin

Country Status (7)

Country Link
US (1) US8759024B2 (de)
EP (1) EP2358893A2 (de)
JP (1) JP5813512B2 (de)
KR (1) KR101739147B1 (de)
CN (1) CN102245781B (de)
DE (1) DE102008063234B4 (de)
WO (1) WO2010075960A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102629304B (zh) * 2012-04-05 2015-02-04 天津大学 基于基因组尺度代谢网络模型的代谢工程设计预测方法
CN102816823B (zh) * 2012-09-18 2014-10-22 江南大学 利用多阶段转速调控策略提高Bacillus subtilis发酵生产核黄素产量的方法
WO2017036903A1 (en) * 2015-08-28 2017-03-09 Dsm Ip Assets B.V. Improved vitamin production
CN108588108B (zh) * 2018-05-04 2021-06-01 湖北大学 一种高效代谢甘油的芽胞杆菌的制备方法和应用
BR112021009177A2 (pt) * 2018-11-15 2021-10-26 Dsm Ip Assets B.V. Produção aprimorada de riboflavina
KR20230168477A (ko) * 2022-06-07 2023-12-14 씨제이제일제당 (주) 리보플라빈 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 리보플라빈을 생산하는 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0821063A2 (de) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Verbesserte Riboflavin-Herstellung
WO2003072785A2 (en) * 2002-02-27 2003-09-04 Dsm Ip Assets B.V. Fermentation process

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1066486C (zh) 1989-06-22 2001-05-30 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
CN1809631B (zh) 2003-06-18 2010-05-26 帝斯曼知识产权资产管理有限公司 用不能形成芽孢的微生物来生产泛酸盐/酯的方法
ATE483726T1 (de) * 2004-12-22 2010-10-15 Dsm Ip Assets Bv Neue gene, die an der gluconeogenese beteiligt sind
US8257944B2 (en) * 2005-11-02 2012-09-04 Dsm Ip Assets B.V. Modified transketolase and use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0821063A2 (de) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Verbesserte Riboflavin-Herstellung
WO2003072785A2 (en) * 2002-02-27 2003-09-04 Dsm Ip Assets B.V. Fermentation process

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt 23 October 2007 (2007-10-23), CHEN X.H. ET AL.: "SubName: Full=CcpC", XP002575051, Database accession no. A7Z428 *
DATABASE UniProt 30 May 2000 (2000-05-30), SCANLAN E., DEVINE K.M.: "RecName: Full=Uncharacterized HTH-type transcriptional regulator ykuM", XP002575050, Database accession no. O34827 *
JOURLIN-CASTELLI C ET AL: "CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 295, no. 4, 28 January 2000 (2000-01-28), pages 865 - 878, XP004465875, ISSN: 0022-2836 *
KIM HYUN-JIN ET AL: "Regulation of the bacillus subtilis ccpC gene by ccpA and ccpC.", MOLECULAR MICROBIOLOGY JAN 2002, vol. 43, no. 2, January 2002 (2002-01-01), pages 399 - 410, XP002575949, ISSN: 0950-382X *
YINGBO ZHU ET AL: "Over-expression of glucose dehydrogenase improves cell growth and riboflavin production in Bacillus subtilis", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DO, vol. 28, no. 20, 16 August 2006 (2006-08-16), pages 1667 - 1672, XP019391630, ISSN: 1573-6776 *
ZHANG F ET AL: "Knockout of the HprK Gene in Bacillus subtilis CcpA Mutant and Its Influence on Riboflavin Fermentation", CHINESE JOURNAL OF BIOTECHNOLOGY,, vol. 22, no. 4, 1 July 2006 (2006-07-01), pages 534 - 538, XP022857381, ISSN: 1872-2075, [retrieved on 20060701] *

Also Published As

Publication number Publication date
KR101739147B1 (ko) 2017-05-23
DE102008063234A1 (de) 2010-06-17
JP5813512B2 (ja) 2015-11-17
DE102008063234B4 (de) 2011-06-22
KR20110104955A (ko) 2011-09-23
CN102245781B (zh) 2015-11-25
EP2358893A2 (de) 2011-08-24
CN102245781A (zh) 2011-11-16
JP2012511898A (ja) 2012-05-31
US8759024B2 (en) 2014-06-24
WO2010075960A8 (de) 2011-06-23
WO2010075960A2 (de) 2010-07-08
US20110312026A1 (en) 2011-12-22

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