WO2010063471A1 - Inhibiteurs de l’accumulation de la protéine hif-1 - Google Patents

Inhibiteurs de l’accumulation de la protéine hif-1 Download PDF

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WO2010063471A1
WO2010063471A1 PCT/EP2009/008632 EP2009008632W WO2010063471A1 WO 2010063471 A1 WO2010063471 A1 WO 2010063471A1 EP 2009008632 W EP2009008632 W EP 2009008632W WO 2010063471 A1 WO2010063471 A1 WO 2010063471A1
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alkyl
phenyl
group
compound according
angiogenesis
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PCT/EP2009/008632
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Philipp Wabnitz
Matthias Gehling
Thomas Henkel
M. Lienhard Schmitz
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Intermed Discovery Gmbh
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Priority to JP2011538897A priority Critical patent/JP2012510964A/ja
Priority to CA2744391A priority patent/CA2744391A1/fr
Priority to AU2009321723A priority patent/AU2009321723A1/en
Priority to MX2011005482A priority patent/MX2011005482A/es
Priority to BRPI0922938A priority patent/BRPI0922938A2/pt
Priority to CN2009801557467A priority patent/CN102300568A/zh
Priority to EP09763878A priority patent/EP2373311A1/fr
Priority to SG2011036688A priority patent/SG171779A1/en
Publication of WO2010063471A1 publication Critical patent/WO2010063471A1/fr
Priority to IL213007A priority patent/IL213007A0/en
Priority to US13/152,365 priority patent/US20120040956A1/en

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    • A—HUMAN NECESSITIES
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    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
    • A61K31/343—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
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    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
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    • A—HUMAN NECESSITIES
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
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    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/77—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D307/93—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems condensed with a ring other than six-membered

Definitions

  • the invention relates to cyclopentabenzofurane derivatives useful for the treatment and/or prophylaxis of angiogenesis-related disorders, preferably pulmonary hypertension.
  • Hypoxia-inducible factor 1 (HIF-1 ) is a transcription factor that regulates the expression of several genes involved in key aspects of adaptive responses to hypoxia, including cellular immortalization, maintenance of stem cell pools, cellular de-differentiation, erythropoiesis, genetic instability, vascularisation, metabolic reprogramming, autocrine growth factor signaling, and invasion/metastasis.
  • the HIF-1 transcription factor is formed as a heterodimer by oxygene-regulated HIF-1 ⁇ and constitutively expressed HIF-1 ⁇ . The latter does also dimerize with the structurally and functionally related HIF-2 ⁇ protein regulating an overlapping battery of target genes.
  • the HIF-1 complex mediates expression of many genes such as e.g. VEGF, EPO, LDH, PDK1 etc. which are considered to be key mediators within the above mentioned biological processes.
  • HIF-1 The transcriptional activity of HIF-1 is closely controlled by a hypoxic stimulus.
  • HIF-1 ⁇ (as well as HIF-2 ⁇ ) as a subject to oxygen mediated prolyl hydroxylation by Prolyl Hydoxylase (PHD) underlies a high turn-over (half-life: ca. 5 min).
  • PHD Prolyl Hydoxylase
  • VHL von Hippel-Lindau tumor suppressor protein
  • VHL von Hippel-Lindau tumor suppressor protein
  • the ubiquitinated HIF-1 ⁇ protein is then degraded by the proteasomal complex.
  • Fig. 1a schematic drawing of HIF-1 protein mediated (pro-angiogenic) activity due to hypoxic or VEGF stimuli).
  • HIF-1 protein dependent transcription will allow specific modulation / treatment of vascularisation and vascular remodelling.
  • Pathological vascularisation and vascular remodelling are associated with multiple human disorders such as e.g. cancer (i.e. tumor vascularisation) or pulmonary hypertension and can be induced by e.g. lack of oxygen (hypoxia; compare Fig. 1a).
  • HIF-1 activity is induced in response to continuous hypoxia, intermittent hypoxia, growth factor stimulation and mediates e.g. maladaptive responses to chronic continuous and intermittent hypoxia, which underlie the development of pulmonary and systemic hypertension (Semenza GL. Physiology (Bethesda), 2009;24:97-106).
  • HIF-1 High-Varela ML et al., Expression of HIF-1alpha, VEGF and EPO in peripheral blood from patients with two cardiac abnormalities associated with hypoxia. Clin Biochem. 2009).
  • HIF-1 has been reported to be regulated oxygen-dependently thereby mediating the adaptive response to changes in tissue oxygenation, see J.J. Haddad, Oxygen-sensing mechanisms and the regulation of redox-responsive transcription factors in development and pathophysiology. Respir Res 2002, 3:26 and G. Semenza, Targeting HIF-1 for Cancer Therapy. NatRevCancer 2003, 3: 721-732.
  • HIF-1 has also been reported to stimulate transcriptional activation of vascular endothelial growth factor (VEGF), a ligand of the VEGF receptor family which in turn stimulates cellular proliferation and angiogenesis.
  • VEGF vascular endothelial growth factor
  • Suppression and loss-of-function of HIF-1 have been reported to be associated with reduced tumor growth, vascularisation and metastasis, see G. Semenza, Evaluation of HIF-1 inhibitors as anticancer agents.
  • HIF-1 overexpression has also been observed in animal models in association with tumor growth, increased vascularisation, and metastasis. Most of locally advanced solid tumors contain regions of reduced oxygen availability. This intratumoral hypoxia results out of the tumor cells distance from a functional blood vessel which hinders the diffusion of adequate amounts of oxygen as a result of rapid cancer cell proliferation and disturbed formation of blood vessels. In the meantime, immunohistochemical detection of HIF-1 ⁇ overexpression in biopsy sections has become a prognostic factor in many cancers. A growing number of novel anticancer agents have been shown to inhibit HIF-1 through a variety of molecular mechanisms (Semenza G. L.; 2007, Drug Discovery Today, Vol. 12, 19/20, 853-859). Determining which combination of drugs to administer to any given patient remains a major obstacle to improving cancer treatment outcomes.
  • HIF-1 inhibition has been shown to have the opposite effect, thus validating HIF-1 as a target for treatment of hypoxia and angiogenesis (neo-vascularization) related disorders.
  • HIF-1 action is located more downstream within a regulatory pathway triggered by e.g. hypoxia and other stimuli such as e.g. VEGF-receptor signal, the point of intervention targeted by compounds of this invention will result in much more specific effects compared to e.g. marketed VEGF-receptor inhibitors such as e.g. Sunitinib; Sorafenib and Avastin (see Fig. 1b).
  • compounds of this invention will be able to influence or modify hypoxia induced physiological signaling more directly and precisely than e.g. VEGF or other Receptor Tyrosine Kinase (RTK) inhibitors.
  • novel therapeutic approaches can be designed, therapies can be optimized (personalized) by e.g. combinatory therapeutic approaches and side effects can be reduced.
  • compounds of this invention will show superior effects compared to biological entities such as antibodies, since the pharmacologically useful action of the compounds of this invention are able to penetrate cellular membranes. Therefore compounds of this invention are able to take their effect inside of mammalian cells while anti-body based effectors will usually not be able to even reach the cells ' interior.
  • Endometriosis does mean the presence of ectopic endometrial tissue outside the uterine cavity.
  • E. is a common disease affecting women during their reproductive years.
  • Hif-1 has been reported to have a role in the regulation of endometrioisis, see Becker et al., 2-Methoxyestradiol Inhibits Hypoxia-Inducible Factor-1 alpha and Suppresses Growth of Lesions in a Mouse Model of Endometriosis. Am J Pathol 2008, 172:534-544.
  • Inhibitors of VEGF and/or HIF-1 (signal) as a mediator of VEGF expression has been described in the art as potential therapeutic approach to treat pulmonary disorders such as e.g. chronic obstructive pulmonary disease (COPD) and pulmonary hypertension, see H. Kanazawa, Role of vascular endothelial growth factor in the pathogenesis of chronic obstructive pulmonary disease. MedSciMonit 2007, 13(11 ): RA189-195.
  • COPD chronic obstructive pulmonary disease
  • HiF-I has been described to be associated with inflammatory processes via hypoxia and angiotensin receptor expression, see G. R. Smith, Cancer, inflammation and the AT1 and AT2 receptors. Journal of Inflammation 2004, 1 :3.
  • HIF-1 has been described in the art as target for therapeutic approaches towards hypoxia- induced kidney fibrosis and ESRD, see M. Nangaku et al., Role of chronic hypoxia and hypoxia inducible factor in kidney Disease. Chinese Medical Journal 2008; 121(3):257-264 257.
  • HIF-1 Up-regulation of HIF-1 has been described in the art to be associated with Peyronie ' s disease, see M. Lucattelli et al., A new mouse model of Peyronie's disease: an increased expression of hypoxia-inducible factor-1 target genes during the development of penile changes, lnt J Biochem Cell Biol. 2008, 40(11):2638-48.
  • HiM alpha overexpression has been associated in the art with erectile dysfunction, see M. Lee et al., Efficient gene expression system using the RTP801 promoter in the corpus cavernosum of high-cholesterol diet-induced erectile dysfunction rats for gene therapy. J Sex Med. 2008 Jun;5(6): 1355-64.
  • HIF-1 overexpression has been described to promote fibrosis, see V.H. Haase, Pathophysiological Consequences of HIF Activation: HIF as a modulator of fibrosis. Ann N Y Acad Sci. 2009,1177:57-65.
  • hypoxia-induced HIF-1 has been described as contributor to the progression of scleroderma, see K.H. Hong et al., Hypoxia induces expression of connective tissue growth factor in scleroderma skin fibroblasts. Clin Exp Immunol. 2006, 146(2):362-70.
  • HIF-1 overexpression due to hypoxia has been clinically associated with ARDS progression, see N. Hirani, The regulation of interleukin-8 by hypoxia in human macrophages— a potential role in the pathogenesis of the acute respiratory distress syndrome (ARDS). MoI Med. 2001 , 7(10):685-97.
  • HIF-1 has been associated with atherosclerosis; see N. Adhikari et al., Transcription factor and kinase-mediated signaling in atherosclerosis and vascular injury. Curr Atheroscler Rep. 2006, 8(3):252-60 and J. C. Sluimer and MJ. Daemen, Novel concepts in atherogenesis: angiogenesis and hypoxia in atherosclerosis. J Pathol. 2009, 218(1 ):7-29. HIF-1 expression has been associated with hernangiobiastorna, see D. Zagzag et ai., Expression of hypoxia-inducible factor 1 alpha in brain tumors: association with angiogenesis, invasion, and progression. Cancer.
  • HIF-1 alpha and HIF-2alpha Up-regulation of hypoxia-inducible factors HIF-1 alpha and HIF-2alpha under normoxic conditions in renal carcinoma cells by von Hippel-Lindau tumor suppressor gene loss of function. Oncogene. 2000, 19(48):5435-43.
  • HIF-1 expression has been described a contributing factor for a metastatic phenotype of tumor cells, see N. Simiantonaki et al., Hypoxia-inducible factor 1 alpha expression increases during colorectal carcinogenesis and tumor progression. BMC Cancer. 2008, 8:320.
  • angiogenesis inhibitors there are known angiogenesis inhibitors already in used as pharmaceuticals in humans.
  • Sorafenib (Nexavar®, Trademark by Bayer Healthcare) has been described in the art as kinase inhibitor that significantly reduces angiogenesis (e.g. tumor vascularisation) by inhibiting the vascular endothelial growth factor (VEGF) receptor, amongst others.
  • VEGF vascular endothelial growth factor
  • Sunitinib (Sutent®, Trademark by Ffizer; formerly distributed as SU 11248) has been described in the art as anti-angiogenic effector that exhibits direct antitumor and anti- angiogenic activity via inhibition of the receptor tyrosine kinases platelet-derived growth factor receptor, vascular endothelial growth factor receptor, KIT, and FLT3, see P. Marzola P et al., Early anti-angiogenic activity of SU11248 evaluated in vivo by dynamic contrast- enhanced magnetic resonance imaging in an experimental model of colon carcinoma. Clin Cancer Res 2005, 11(16): 5827-32 and S.
  • VEGF receptor inhibitors are Vandetanib (Zactima®, Trademark by AstraZeneca; formerly distributed as ZD6474), AZD2171 (Recentin®, Trademark by AstraZeneca) and the anti-body Bevacizumab (Avastin®, Trademark by Genentech/Roche).
  • HIF inhibitors used as HIF inhibitors are not satisfactory in every respect and thus, there is a demand for further HIF inhibitors, especially for HIF-1 inhibitors being useful for the treatment of hypoxia and angiogenesis (neo-vascularisation) related disorders. It is an object of the invention to provide compounds that have advantages over the compounds of the prior art. The compounds should effectively inhibit HIF at comparatively low doses and should be useful for the treatment and/or prophylaxis of angiogenesis-related disorders.
  • cyclopentabenzofuranes exhibit HIF inhibitory activity.
  • These cyclopentabenzofuranes can be i.e. derived from a class of natural products which are referred to as rocaglaols or rocaglamides which can be i.e. extracted from various species of the Aglaia plant.
  • cyclopentabenzofurane derivatives A number of cyclopentabenzofurane derivatives is known from the prior art that i.e. exhibit an inhibitory activity against the NF-KB transcription factor which occupies a central role in inflammatory processes and carcinogenesis.
  • cyclopentabenzofuranes are known as potent anticancer agents (King, M. A. et al., J. Chem. Soc, Chem. Commun. 1982: 1150-1151 ), i.e. as anti-leukaemia agents (Lee, S. K. et al., Chem. Biol. Interaet. 1998, 115: 215-228; US 4,539,414).
  • cyclopentabenzofurane derivatives are also known to be useful for the treatment of pain (WO 2008/014066) and for the treatment of inflammatory and/or autoimmune diseases (EP 1 693 059; WO 2005/113529; WO 2006/129318).
  • WO 01/12592 discloses hydroxamic acid compounds useful as matrix metalloproteinase inhibitors.
  • EP 1 016 408 relates to the use of certain C-C chemokine production inhibitors for maufacture of a medicament for certain disorders including chronic intractable inflammation and chronic rheumatoid arthritis.
  • HIF-1 dependent luciferase transcription has demonstrated very potent (low nM) inhibition of HIF-1 dependent luciferase transcription and single digit nanomolar inhibition of HIF-I dependent target gene transcription (e.g. PDK1). It was demonstrated that these effects were not mediated on the transcriptional (mRNA) level and can be reached in a therapeutic manner (compound application after signal induction). Furthermore it was demonstrated that the HIF- dependent effects were triggered by a potent inhibition of HIF-1 ⁇ protein itself. This effect was shown to be HiMa protein specific and not a result of un-specific inhibition of translational processes. In vitro the compounds of this invention did show a potent inhibition of HUVEC sprouting (angiogenesis; vascular modeling) at two-digit nanomolar IC 50 S.
  • VEGF receptor inhibitors such as e.g. Sunitinib (Sutent®) and Sorafenib (Nexavar®).
  • VEGF / Receptor Tyrosine Kinase inhibitors or other upstream Modulators of HIF-1 signaling such as e.g. diarrhea, eczema, hair loss, hemorrhage, hypertension, hypothyroidism, nausea, emesis, erythema, itchiness, fatigue, pain and increased amylase and lipase activity.
  • VEGF / Receptor Tyrosine Kinase inhibitors or other upstream Modulators of HIF-1 signaling such as e.g. diarrhea, eczema, hair loss, hemorrhage, hypertension, hypothyroidism, nausea, emesis, erythema, itchiness, fatigue, pain and increased amylase and lipase activity.
  • a first aspect of the invention relates to compounds of general formula (I)
  • R 5 and R 6 are phenyl
  • R 8 is -H; -OH; -O-C 1-8 -alkyl; -O-phenyl; -NH 2 ; -NH-C 1-8 -alkyl; -N(C 1-8 -alkyl) 2 ;
  • R 10 and R 11 are -H
  • R 15 is -C 1-8 -alkyl; -OH; -O-C 1-8 -alkyl; or -O-phenyl;
  • the dotted line is a single or a double bond, wherein in case of a double bond R 12 is not existing; R 12 is -H or -Ci- 3 -alkyl;
  • R 13 is -H; -C ⁇ -alkyl; -OH; -O-C 1-8 -alkyl; or -O-phenyl;
  • R 14 is -H, -C 1- ⁇ -alkyl
  • angiogenesis-related disorders preferably selected from the group consisting of diseases of the urogenital tract, eye diseases, lung diseases, kidney diseases, osteoarthritis and rheumatic disorders; particularly preferably pulmonary hypertension.
  • alkyl or “Ci- 8 -alkyl” refers to a saturated or unsaturated, linear or branched and/or cyclic hydrocarbon.
  • alkyl encompasses “alkyl”, “alkenyl” and “alkynyl” as well as “cycloalkyl”, “cycloalkenyl” and “cycloalkynyr.
  • Examples of preferred aikyi residues are methyl, ethyl, n- ⁇ ro ⁇ yi, i-propyl, n- butyl, sec-butyl, iso.-butyl, tert.-butyl, n-pentyl, n-hexyl, n-heptyl and n-octyl.
  • Examples of preferred alkenyl residues include vinyl, allyl and butadienyl.
  • Examples of preferred alkynyl residues include ethynyl and propargyl.
  • a skilled person recognizes that a cyclic hydrocarbon requires the presence of at least 3 ring atoms.
  • Examples of preferred cycloalkyl residues are cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
  • -NH(Ci ⁇ -alkyl) is selected from the group consisting of -NH(CH 3 ), -NH(CH 2 CH 3 ) and -NH(CH(CH 3 J 2 ).
  • -N(C 1-8 -alkyl) 2 is selected from the group consisting of -N(CH 3 J 2 , -N(CH 2 CH 3 ) 2 , N(CH(CH 3 ) 2 ) 2 and -N(C 3 H 7 J 2 .
  • Examples of preferred substituted alkyl residues are -CF 3 , -CH 2 CH 2 -OCH 3 , -CH 2 CH 2 NH 2 , -CH 2 CH 2 - NH(CH 3 ), -CH 2 CH 2 -N(CH 3 J 2 , -CH 2 CH 2 -NH(CH 2 CH 3 ), -CH 2 CH 2 -N (CH 2 CH 3 ) 2 , -CH 2 -CH 2 -pyrrolidinyl, -CH 2 CH 2 CH 2 -pyrrolidinyl, -CH 2 CH 2 CH 2 -azetidinyl and -CH 2 CH 2 CH 2 -aziridinyl.
  • preferred heterocyclyls or heterocycles are aziridinyl, azetidin
  • carbohydrate or “Cs- ⁇ -carbohydrate” refer to a mono or disaccharide consisting of one or two pentoses (C 5 -carbohydrate or C 10 -carbohydrate) and/or one or two hexoses (C 6 -carbohydrate or C 12 -carbohydrate), each optionally in their desoxy forms, the disaccharides in each form connected to each other via a glycosidic bond, unsubstituted or substituted with one, two, three, four or five substituents independently selected from the group consisting of methyl, ethyl, acetyl, benzoyl or 3,4,5-trihydroxy- benzoyl.
  • Examples of preferred pentoses are xylose, arabinose, each in the pyranosidic or furanosidic form.
  • Examples of preferred hexoses are glucose, 6-deoxyglucose, rhamnose, each in the pyranosidic of furanosidic form.
  • Examples of preferred glycosidic connections are 1 ⁇ 4 and 1—>6.
  • "Carbohydrate” or "C 5 -i 2 -carbohydrate” residues are bound to the higher general formula via one of its oxygen atoms.
  • Physiologically acceptable salts of the compounds of general formula (I) include salts with physiologically acceptable acids as well as salts with physiologically acceptable bases.
  • Physiologically acceptable acids include inorganic acids such as HCI, HBr, H 2 SO 4 , H 3 PO 4 and the like; and organic acids such as formic acid, acetic acid, propionic acid, citric acid, maleic acid, malic acid, lactic acid, fumaric acid, and the like.
  • Physiologically acceptable bases include ammonia, and organic amines.
  • the invention also relates to the stereoisomers of the compounds of general formula (I), such as enantiomers or diastereomers, tautomeric forms, salts, solvates such as hydrates, polymorphs, and the like.
  • R 1 and R 2 or R 2 and R 3 or R 3 and R 4 together with the two carbon atoms they are bound to form a five-membered ring with -0-CH 2 -O- or a six-membered ring with -0-CH 2 -CH 2 -O-, while the other radicals R 1 to R 4 are independently selected from those mentioned above;
  • R 8 is H; -OH; -O-C 1-8 -alkyl; -O-phenyl; -NH 2 ; -NH-C 1-8 -alkyl; -N(C ⁇ -alkyl) 2 ;
  • R 10 and R 11 are -H
  • R 15 is -C 1-8 -alkyl; -OH; -O-d- ⁇ -alkyl; or -O-phenyl.
  • Preferred compounds of general formula (I) are of general formulae (Ia), (Ib), (Ic) or (Id):
  • R 5 and R 6 are phenyl
  • R 7 and R 8 are independently of each other -OH, or -O-C 1-8 -alkyl
  • R 39 a , D R1 l 0 u ⁇ a n ndA DR1"1 are -H.
  • R 7 is -OH, or -O-C 1-8 -alkyl
  • R 8 is OH
  • R 9 , R 10 and R 11 are -H.
  • R 5 and R 6 are phenyl
  • R 7 and R 8 are OH
  • R 9 , R 10 and R 11 are -H.
  • R 2 and R 4 are H
  • R 5 and R 6 are phenyl
  • R 7 and R 8 are independently of each other -OH 1 or -O-C ⁇ -alkyl
  • R 9 , R 10 and R 11 are -H.
  • R 2 and R 4 are -H
  • R 5 and R 6 are phenyl
  • R 7 and R 8 denote -OH
  • R 9 , R 1 ⁇ and R 11 are -H.
  • R 1 and R 3 independently of each other denote -H; -OH; -O-C 1-8 -alkyl; O-phenyl; -O-C 1-8 -alkyl- phenyl;
  • R ⁇ and R 4 are -H
  • R 5 and R 6 are phenyl
  • R 7 and R 8 are -OH
  • Another preferred compound of genera! formula (! is of genera! formula (Ie).
  • R 1 denotes H; -O-Ci- ⁇ -alkyI, unsubstituted or substituted with one substituent selected from the group consisting Of -OCH 3 , -OCH 2 CH 3 , -NH 2 , -NH(CH 3 ), -NH(CH 2 CH 3 ), -N(CH 3 J 2 ,
  • R 3 denotes -OH; -O-phenyl, unsubstituted; -O-Ci ⁇ -alkyl, unsubstituted or substituted with one substituent selected from the group consisting of -F, -Cl, -OCH 3 , -OCH 2 CH 3 , -NH 2 , - NH(CH 3 ), -NH(CH 2 CH 3 ), -NH(CH(CH 3 ) 2 ), -NH(C(CH 3 J 3 ), -NH(CH 2 -phenyl) or -NH(phenyl), wherein phenyl in each case is unsubstituted, -N(CH 3 ) 2 , -N(CH 2 CH 3 J 2 , ⁇ -o CH 2 -phenyl, unsubstituted;
  • R 5 and R 6 are phenyl, unsubstituted, or substituted with one, two or three substituents independently of each other selected from the group consisting of -F, -Cl 1 -Br, -I, -OH, -OCH 3 , -NH 2 , -CH 3 and -CF 3 .
  • R 1 is selected from -H and -OCH 3 ;
  • R 31 is selected from -H; -CH 2 NHCH 3 ; -CH 2 N(CH 3 ) 2 ; and -CH 2 - substituted with ⁇ N J; R a is -F, -Cl or -OCH 3 ; and R d -H, -F or -Cl.
  • Particularly preferred compounds of one of the general formulae (I), (Ia), (Ib), (Ic), (Id), (Ie), (If) or (Ig) are selected from the group consisting of (1 ,3,3a,8b)-3-(3-fluorophenyl)-6,8-dimethoxy-3a-(4-methoxyphenyl)-2,3,3a,8b-tetrahydro-1H- benzo[d]cyclopenta[b]furan-1 ,8b-diol (for the purpose of the specification also referred to as • ⁇ MD-019064”):
  • a further aspect of the invention relates to the use of at least one compound of general formula (I), preferably of general formula (1a), (1 b), (1c), (1e), (1f) or (1g), according to the invention for the manufacture of a medicament for the treatment and/or prophylaxis of angiogenesis-related disorders, preferably pulmonary hypertension. All preferred embodiments of the compound of general formula (I) also apply to the medicament according to the invention and thus, are not repeated here below.
  • the medicament is a pharmaceutical composition comprising at least one compound of general formula (I) according to the invention as described above and a physiologically acceptable carrier.
  • a further aspect of the invention relates to the use of at least one compound of general formula (I) according to the invention for the manufacture of a pharmaceutical composition as described above for the treatment and/or prophylaxis of angiogenesis-related disorders preferably pulmonary hypertension. All preferred embodiments of the compound of general formula (I) also apply to the pharmaceutical composition according to the invention and thus, are not repeated here below.
  • the pharmaceutical composition according to the invention may be liquid, e.g. a solution, dispersion, suspension or emulsion; or solid, e.g. a powder, paste, gel, and the like.
  • Suitable physiologically acceptable carriers are known to the person skilled in the art.
  • Suitable liquid carriers include water, ethanol and the like.
  • Suitable solid carriers include typical pharmaceutical excipients, such as fillers, binders, glidants, disintegrants, and the like. In this regard it can be referred to, e.g., D. E. Bugay et al., Pharmaceutical Excipients, lnforma Healthcare; 1 edition (December 1 , 1998).
  • the pharmaceutical composition according to the invention may contain inert non-toxic pharmaceutically suitable auxiliaries, such as for example excipients, solvents, vehicles, emulsifiers and/or dispersants.
  • auxiliaries can be mentioned as examples: water, solid excipients such as ground natural or synthetic minerals (e.g. talcum or silicates), sugar (e.g. lactose), non-toxic organic solvents such as paraffins, vegetable oils (e.g. sesame oil), alcohols (e.g. ethanol, glycerol), glycols (e.g. polyethylene glycol), emulsifying agents, dispersants (e.g. polyvinylpyrrolidone) and lubricants (e.g. magnesium sulphate).
  • ground natural or synthetic minerals e.g. talcum or silicates
  • sugar e.g. lactose
  • non-toxic organic solvents such as paraffins, vegetable oils (e.g. sesame oil), alcohols (e.g. ethanol, glycerol), glycols (e.g. polyethylene glycol), emulsifying agents, dispersants (e.g. polyvinylpyrrolidone) and
  • the relative weight ratio of the compound of general formula (I) and the physiologically acceptable carrier is preferably within the ratio of from 99.9 : 0.1 to 0.1 : 99.9.
  • a further aspect of the invention relates to the use of at least one compound of general formula (I) according to the invention for the manufacture of a pharmaceutical dosage form containing the pharmaceutical composition as described above for the treatment and/or prophylaxis of angiogenesis-related disorders. All preferred embodiments of the compound of general formula (I) also apply to the pharmaceutical dosage form according to the invention and thus, are not repeated here below.
  • the pharmaceutical dosage forms according to the invention may be adapted, e.g., for systemic, local or topical administration.
  • Systemic administration includes, e.g., intraveneous, inhalative or oral administration.
  • the compounds according to the invention can exhibit non-systemic or systemic activity, wherein the latter is preferred.
  • systemic activity the pharmaceutical dosage forms containing the active compounds can be administered, among other things, orally, parenterally, or inhalatory, wherein oral administration is preferred.
  • non-systemic activity the pharmaceutical dosage forms containing the active compounds can be administered, among other things, topically.
  • compositions for administration to the mucous membranes i.e. buccal, lingual, sublingual, rectal, nasal, pulmonary, conjunctival or intravaginal
  • Administration can be carried out by avoiding absorption (i.e. intracardiac, intra-arterial, intravenous, intraspinal or intralumbar administration) or by including absorption (i.e. intracutaneous, subcutaneous, percutaneous, intramuscular or intraperitoneal administration).
  • the pharmaceutical dosage forms containing the active compounds can be administered per se or in pharmaceutical dosage forms (administration forms).
  • Suitable pharmaceutical dosage forms for oral administration are, inter alia, normal and enteric-coated tablets, capsules, coated tablets, pi ⁇ s, granules, pellets, powders, solid and liquid aerosols, syrups, emulsions, suspensions and solutions.
  • Suitable pharmaceutical dosage forms for parenteral administration are injection and infusion solutions.
  • the active compound can be present in the pharmaceutical dosage forms in concentrations of from 0.001-100% by weight; preferably the concentration of the active compound should be 0.5-90% by weight, i.e. quantities which are sufficient to allow the specified range of dosage.
  • tablets can of course also contain additives such as sodium citrate as well as additives such as starch, gelatin and the like.
  • Flavour enhancers or colorants can also be added to aqueous preparations for oral administration.
  • the quantity is about 0.001 to 100 mg/kg, preferably about 0.1 to 50 mg/kg of body weight.
  • the pharmaceutical dosage forms may exhibit, e.g., an immediate or a sustained release profile of the active compound contained therein.
  • the compounds of the invention are inhibitors of HIF- 1 protein accumulation, and can therefore be used for the manufacture of a medicament intended to inhibit HIF-1 protein accumulation.
  • the compounds according to the invention exhibit an unforeseeable, useful pharmacological and pharmacokinetic activity spectrum. They are therefore suitable for use as medicaments for the treatment and/or prophylaxis of disorders in humans and animals.
  • the compounds of the general formula (I) are HIF inhibitors and therefore suitable for the treatment and/or prophylaxis of a variety of angiogenesis-related disorders or are useful to prepare a medicament for the treatment and/or prophylaxis of angiogenesis-related disorders.
  • the compounds of general formula (I) according to the invention can be used for the treatment and/or prophylaxis of angiogenesis-related disorders or are useful to prepare a medicament for the treatment and/or prophylaxis of angiogenesis-related disorders.
  • Angiogenesis-related disorders are preferably selected from the group consisting of diseases of the urogenital tract, preferably non-inflammatory diseases of the female genital tract, endometriosis of the uterus, endometriosis of the ovary, endometriosis of tuba uterine, endometriosis of the intestine, endometriosis of scars, endometriosis of septum recto- vaginale, endometriosis of the vagina, and endometriosis of the pelvis peritoneum; eye diseases, preferably macular degeneration, vitelliform dystrophy (Best disease), retinopathies, diabetic retinopathy, glaucoma, neuroscular glaucoma, choroidal neovascularisation, occult choroidal neovascularisation, neovascularisation of the cornea, retrolental fibroplasias, and rubeosis irridis; lung diseases, preferably airway remodelling
  • the compounds are for treatment or prevention of pulmonary disorders selected from the group consisting of pulmonary hypertension, pulmonary sarcoidosis, and idiopathic pulmonary fibrosis.
  • pulmonary disorders selected from the group consisting of pulmonary hypertension, pulmonary sarcoidosis, and idiopathic pulmonary fibrosis.
  • pulmonary hypertension refers to any pulmonary hypertension, pulmonary sarcoidosis, and idiopathic pulmonary fibrosis, respectively, irrespective of whether they are angiogenesis- related or not, if any.
  • angiogenesis-related disorders are selected from the group consisting of diseases of the urogenital tract, preferably non-inflammatory diseases of the female genital tract, endometriosis of the uterus, endometriosis of the ovary, endometriosis of tuba uterine, endometriosis of the intestine, endometriosis of scars, endometriosis of septum rectovaginale, endometriosis of the vagina, and endometriosis of the peritoneum.
  • diseases of the urogenital tract preferably non-inflammatory diseases of the female genital tract, endometriosis of the uterus, endometriosis of the ovary, endometriosis of tuba uterine, endometriosis of the intestine, endometriosis of scars, endometriosis of septum rectovaginale, endometriosis of the vagina, and endometriosis of the
  • angiogenesis-related disorders are selected from the group consisting of eye diseases, preferably selected from the group consisting of macular degeneration, vitelliform dystrophy (Best disease), retinopathies, diabetic retinopathy, glaucoma, neuroscular glaucoma, choroidal neovascularisation, occult choroidal neovascularisation, neovascularisation of the cornea, retrolental fibroplasias and rubeosis irridis.
  • eye diseases preferably selected from the group consisting of macular degeneration, vitelliform dystrophy (Best disease), retinopathies, diabetic retinopathy, glaucoma, neuroscular glaucoma, choroidal neovascularisation, occult choroidal neovascularisation, neovascularisation of the cornea, retrolental fibroplasias and rubeosis irridis.
  • angiogenesis-related disorders are selected from the group consisting of lung diseases, preferably selected from the group consisting of airway remodelling, COPD (chronic obstructive respiratory disorder), ARDS (acute respiratory distress syndrome), infant respiratory distress syndrome, pulmonary hypertension, pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
  • COPD chronic obstructive respiratory disorder
  • ARDS acute respiratory distress syndrome
  • infant respiratory distress syndrome pulmonary hypertension
  • pulmonary sarcoidosis idiopathic pulmonary fibrosis.
  • angiogenesis-related disorders are selected from the group consisting of kidney diseases, preferably nephropathies, chronic hypoxia induced diseases, ESRD, renal fibrosis, renal artery stenosis, and glomerulonephritis.
  • angiogenesis-related disorders are selected from the group consisting of osteoarthritis, preferably gonarthrosis, coxarthrosis, polyarthrosis, rhizarthrosis, and further arthroses.
  • angiogenesis-related disorders are selected from the group consisting of rheumatic disorders, preferably rheumatoide arthritis.
  • the compounds according to general formula (I) of the invention can be synthesized by various routes.
  • the compounds can be prepared fully synthetically, starting from building blocks that are commercially available.
  • the compounds according can be isolated from plants, preferably from various species of the Aglaia plant or the precursor products isolated from said plants can be used as starting materials in the synthesis (semi-synthetic route).
  • compounds of general formula (I) can be obtained by isolation, by semi-synthetic derivatization of the compounds obtained by isolation or by synthesis following previously published or new synthetic route.
  • the compounds according to the invention can be, e.g., natural products, derivatives of these natural products or total synthetic analogs.
  • HIF-1 does describe the protein while “Hif-1” describes the gene / mRNA.
  • a HIF-1 -dependent luciferase reporter gene was transfected into 293T cells by transfection with RotifectTM (Trademarkd by Carl Roth GmbH; Düsseldorf, Germany) or alternatively into Jurkat T cells by electroporation. Both cell lines were transfected with a HIF-1 -dependent reporter gene. The next day, cells were preincubated for 1 h with the indicated, submicromolar concentrations of the test comounds and then incubated for further 8 h under normoxic or hypoxic (1% O 2 ) conditions. After that, cells were harvested followed by the analysis of iuciferase activity in a iuminometer.
  • Example 2 IC 50 value determination of compounds with HIF-1 luciferase inhibitory activity.
  • a HIF-1-dependent luciferase reporter gene was transfected into 293T cells. The next morning, cells were pretreated for 1 h with the indicated concentrations of compounds, followed by induction of hypoxia (hypoxia chamber) for 8 h. Subsequently cells were harvested and lyzed. Luciferase activity in cell extracts was measured in a luminometer (Duo Lumat LB 9507, Berthold) by injecting 20 microliter of assay buffer to 20 microliter of extract. Light emission was measured for 10 s, relative light units are given. The results are presented in Fig. 3 and show the IC 50 values of 19, 18, and 23 nM which were found for IMD- 026259, IMD-026260 and IMD-019064, respectively.
  • IMD-019064; IMD-026259 and IMD-026260 were shown to be able to inhibit HIF-1- dependent luciferase expression even when added 4 hours after hypoxic induction. These results demonstrate that the compounds of the invention do not only prevent the induction of the HIF-1 response but also interfere with ongoing HIF-1-dependent transcription when the protein is already stabilized (Fig. 4).
  • Example 4 Effect of compounds on Hif-1 mRNA expression.
  • HiMa The expression of HiMa was quantified and results were normalized to Hprti and ⁇ -Actin ("housekeeping-gene") expression. The relative abundance of the different genes was calculated by the comparative CT method.
  • HIF-1 ⁇ transcription of normoxic control cells was arbitrarily set to 1.
  • a representative experiment is displayed in Fig. 5. While hypoxia moderately increased HIF-1 ⁇ transcription by a factor of -3 under the conditions employed, the quantification of HIF-1 ⁇ mRNA expression by qPCR revealed no significant effect of the tested compounds.
  • the tested substances (I M D-019064; IMD-026259 and IMD-026260) did not show any effects on HiMa mRNA production - neither under normoxic nor under hypoxic conditions. Therefore an effect of IMD-compounds on HIF-1-dependent gene expression seems not to be mediated on HIF-1 ⁇ transcription (Fig. 5).
  • Example 5 Effect of IMD compounds on HIF-I target gene expression.
  • Human 293T cells were cultivated either under normoxic conditions or under hypoxic conditions (1% oxygen) for 4 and 8 hs respectively. Cells were harvested, followed by the isolation of RNA and the generation of cDNAs from Oligo (dT)20 primers using a Reverse Transcriptase kit. Then gene expression was measured by real-time PCR for the following two selected HIF-1 ⁇ target genes: LDH-A (lactate dehydrogenase isoform A), and PDK1 (pyruvate dehydrogenase kinasel ). Real-time PCR was performed with cDNA using specific primers and the SYBR Green I detection chemistry system (Applied Biosystems), utilizing an ABI Prism 7300 system.
  • LDH-A lactate dehydrogenase isoform A
  • PDK1 pyruvate dehydrogenase kinasel
  • Actin was also measured as an internal control.
  • the relative abundance of the different genes was calculated by the comparative CT method.
  • gene expression under normoxic conditions was arbitrarily set to 1 for each of the three genes.
  • Two independent experiments confirmed PDK1 as the gene which was induced most prominently compared to LDH. The results are shown in Figure 6a.
  • the IC50 values were determined to range from 8 to 12 nM (IMD 026259, 9 nM; IMD 026260, 8 nM; and IMD 019064, 12 nM), indicating that endogenous genes (e.g. PDK1 ) are even more potently suppressed than the reporter genes (e.g. luciferase).
  • endogenous genes e.g. PDK1
  • reporter genes e.g. luciferase
  • IMD-019064, IMD-026259 and IMD-026260 were demonstrated.
  • Example 6 Suppression of hypoxia induced HIF-1 ⁇ protein accumulation.
  • IMD-026259, IMD-026260 and IMD-019064 on the stabilisation/accumulation of HIF-1 ⁇ protein were investigated.
  • 293T cells were preincubated with the indicated concentrations of all three compounds. After 1 h of pre-incubation, 293T cells were preincubated with the indicated concentrations of compounds and then further incubated under normoxic or hypoxic (4 h, 1% O 2 ) conditions respectively. After lysis of cells in 1 x SDS sample buffer and sonication, the samples were analyzed for HIF-1 ⁇ abundance by immunoblotting. Equal amounts of protein contained in lysates were separated by reducing SDS-PAGE and then further analyzed by immunoblotting with antibodies specifically recognizing HIF-1 ⁇ and the loading control Actin (demonstrating loading of equal protein amounts).
  • HIF-1 ⁇ protein As depicted in Fig. 7, the accumulation of HIF-1 ⁇ protein was not detectable at normoxic conditions (-) while being induced by hypoxia (+). The HIF-1 ⁇ protein accumulation was significantly and specifically prevented at compound concentrations of ⁇ 50 nM. The concentration of 250 nM of IMD-019064 and IMD-026260 did almost completely block HIF-1 ⁇ protein accumulation.
  • Example 7 Inhibition of cell-free protein translation.
  • IMD-019064 on cell-free protein translation in vitro was investigated (see also assay principle in Fig. ⁇ ).
  • Brome mosaic virus (BMV) mRNA encoding for 4 different viral proteins was incubated with ribosomes in the presence and absence of IMD-019064 (Fig. 9a), IMD-026259, IMD-026260 (Fig. 9b), or cycloheximide (Chx, 10 ⁇ M) (positive control) and the formed proteins analyzed by SDS-PAGE.
  • BMV Brome mosaic virus
  • IMD-019064 did not affect protein synthesis in vitro whereas cycloheximide potently suppressed formation of newly synthesized proteins (Fig. 9a, lane: Chx).
  • Panels show representative SDS- PAGE protein gels.
  • Vehicle-containing translation reactions (Veh) represent 100% translation efficacy, while Cycloheximide (Chx; 10 ⁇ g/ml) a known inhibitor of protein translation that was used as a positive control does show significant protein reduction.
  • Example 8 Inhibition of cell-free protein translation.
  • the TNT ® Coupled Reticulocyte Lysate Systems (cell free protein expression) was used to test any potential effects on translation.
  • Flag-tagged NF- ⁇ B p50 protein was produced in vitro.
  • the reticulocyte system was used including 100 nM of the respective compounds (I M D-019064; IMD-026259 and IMD-026260) and DMSO as a solvent control.
  • Example 9 Specific inhibition of signal-dependent amino acid incorporation in cells.
  • IMD-019064 a rocaglaol derivative
  • IMD-019064 a direct inhibitor of protein synthesis
  • Cellular assays incorpororation of radiolabeled amino acids into unstimulated and IL-1b-stimulated endothelial cells
  • activated signaling cascades e.g. IL-1 signalling pathway
  • 293T cells were grown in the presence of the known translation inhibitor cycloheximide (10 ⁇ g/ml) and 100 nM of IMD-019064, IMD-026259 and IMD-026260 respectively.
  • the latter dose was chosen as it represents a concentration that is well above the IC50 values (approx. 5 to 10-fold) from gene expression studies (compare e.g. Examples 2 and 5) but also below concentrations that may cause non-specific effects.
  • Cells were harvested after 10 h and 24 h and cell lysates were produced. Equal amounts of protein contained in the cell lysates were used for immunoblotting with anti-IKK ⁇ /NEMO antibodies as shown in Fig. 12. IKK ⁇ /NEMO is a constitutively expressed relatively labile protein.
  • Example 11 Suppression of angiogenic sprouting in HUVEC cells after hypoxic stimulus.
  • spheroids were prepared as described by Korff and Augustin: J Cell Biol 143: 1341-52, 1998) by pipetting 500 endothelial cells (EC) in a hanging drop on plastic dishes to allow overnight spheroid aggregation. 50 EC spheroids were then embedded in 0.9 ml of a 3D collagen matrix and pipetted into individual wells of a 24 well plate to allow polymerization.
  • the test compound, for HUVEC in combination with Deferoxamine [100 ⁇ M; induction of chemically induced hypoxia] was added after 30 min by pipetting 100 ⁇ l of a 10-fold concentrated working dilution on top of the polymerized gel (see Table 2 for final compound concentrations):
  • Table 2 Final compound concentrations [M]. Plates were incubated at 37°C for 24 hours and fixed by adding 4 % paraformaldehyde. The cumulative sprout length of 10 randomly selected spheroids per data point was analyzed and the relative inhibition by the test compound determined. Fitting of IC 5O curves and calculation of IC 50 values was performed with GraphPad Prism 5.01. Sprouting intensity of EC and fibroblast spheroids was quantitated by an image analysis system using an inverted microscope and the digital imaging software Analysis 3.2 (Soft imaging system, M ⁇ nster, Germany). In parallel NHDF (fibroblast) spheroids were embedded in a 3D collagen gel and treated for 24h with different concentrations of IMD-019064. The cumulative sprout length of 10 randomly selected spheroids per data point was analyzed according to the procedure used for the HUVEC experiment.
  • the test compound IMD-019064 inhibits human umbilical vein endothelial cell (EC) sprouting and fibroblast scattering stimulated by chemically [Deferoxamine; 100 ⁇ M] induced hypoxia in a dose-dependent manner in the spheroid-based angiogenesis assay using a collagen matrix. Deferoxamine induced sprouting of HUVEC spheroids was significantly inhibited by IMD-019064 treatment. An IC 50 value of 30 nM could be determined (Fig. 13). NHDF (fibroblast) spheroid sprouting was found to be inhibited by IMD-019064 by an IC 50 value of 180 nM (Fig. 13). The findings did show a difference in HUVEC and fibroblast sensitivity against IMD-019064 by factor six. This demonstrates that inhibition of hypoxia induced angiogenic effects (HUVEC sprouting) is independent from unspecific cytotoxicity.
  • EC umbilical vein endothelial cell
  • Table 3 Final compound concentrations [M] as used in hypoxia (Deferoxamine)-induced HUVEC sprouting assays.
  • Results are presented in figure 13, respectively, indicating an IC 50 value of 13 nM (Fig. 13: Hypoxia induced EC sprouting) and an IC 50 value of 180 nM (Fig. 13: NHDF fibroblast scattering).
  • Fig. 13 Hypoxia induced EC sprouting
  • Fig. 13 NHDF fibroblast scattering
  • Example 12 Suppression of angiogenic sprouting in HUVEC cells after VEGF-A stimulus.
  • HUVEC spheroids were generated, cultivated and embedded in a 3D collagen gel and stimulated with Vascular Endothelial Growth Factor alpha [VEGF-A; 25 ng/ml] instead of deferoxamine.
  • Cell spheroids were treated for 24h with different concentrations of IMD-026259; IMD-026260; Sutent® (Sunitinib) and Sorafenib (Nexavar®) (in Molar [M], as indicated in Table 4; see also Fig. 14A).
  • Table 4 Final compound concentrations [M] as used in VEGF-induced HUVEC sprouting assays.
  • IMD-019064; IMd-026259 and IMD-026260 act as potent inhibitors of angiogenic sprouting in vitro. Furthermore it was demonstrated that the compounds of the invention have a potent inhibitory effect on HUVEC sprouting (neo- vascularisation) in vitro is superior to that of Sutent® (Sunitinib) and Sorafenib (Nexavar®).
  • Example 13 Evaluation of the anti-angiogenic efficacy of anti-angiogenic test items in the spheroid-based in vivo angiogenesis assay.
  • ECs Human endothelial cells
  • HUVECs Human endothelial cells
  • SMCs smooth muscle cells
  • NHDFs fibroblasts
  • the transplanted human ECs EC spheroids in combination with ECs in suspension
  • form a complex and perfused three dimensional network of capillaries of human origin that is anastomosed (connected with) with the mouse vasculature and host (mouse) pericyte-covered.
  • the quality of the newly formed vasculature is monitored by micro vessel density counting.
  • HUVEC spheroids were prepared as described (Korff and Augustin: J Cell Biol 143: 1341-52, 1998) by pipetting 100 ECs in a hanging drop on plastic dishes to allow overnight spheroid formation. The following day EC spheroids were harvested and mixed in a Matrigel/fibrin solution with suspended HUVECs, SMCs and NHDFs. The final mixture to be used as a plug contained 100.000 spheroid ECs and 200.000 single suspended ECs 1 300.000 SMCs and 100.000 NHDFs. SCID mice were subcutaneously injected with 500 ⁇ l of the cell/matrix suspension.
  • the first perfused vessels are usually detected at day 4 to day 6. After 20 days of in vivo growth a well established vasculature with around 50 - 60 % pericyte-covered and perfused vessels is usually observed.
  • the SCID mice were treated with vehicle or with IMD-026260 (0,3 mg/ml; group 3) respectively, applied p.o. every 3 rd day.
  • mice were sacrificed by cervical dislocation and the Matrigel® (Trademark by BD Biosciences) plugs were removed.
  • the Matrigel® plugs were photographed and fixed in 4% Roti-Histofix (Roth, Düsseldorf, Germany) at room temperature for 4-12h. Thereafter the plugs were paraffin embedded using the semi-enclosed tissue processor Leica TP1020.
  • paraffin sections were prepared from all plugs. Blood vessel formation was detected by staining the sections for human CD34 (NCL-END, Menarini, Berlin, Germany). Three sections per plug were analysed and three images were taken from each section at a magnification of 20Ox using the Eclipse TE2000-U microscope (Nikon, Kanagawa, Japan). The area analyzed per section (three images) was 0.44 mm 2 . The vessel number (CD34 positive) was manually determined using the NIS-elements basic research software (Nikon, Kanagawa, Japan).
  • Example 14 Reduction of neurological damage after ischemic injury (STROKE model).
  • Rats (Male Rj: Sprague-Dawley rats, weighing 250 - 350 g) are placed under isoflurane anaesthesia (5% for induction and 2% for maintenance, under 30% O2). Body temperature is monitored with a rectal temperature probe and maintained with a heating pad at 37°C ⁇ 1 0 C throughout the experiment.
  • Cerebral blood flow is continuously recorded by laser Doppler flowmetry (Moor Instruments MoorLAB) during a period covering induction of cerebral ischemia (from 10-15 minutes before and 5 minutes after MCAo). Under an operating microscope, a skin incision is made between the orbit and the ear and the temporal muscle is dissected. The laser Doppler probe is placed on the right lateral face of the skull.
  • the right common carotid artery (CCA), the external carotid artery (ECA) and the internal carotid artery (ICA) are isolated from adjacent veins and nerves.
  • the CCA is then ligatured and the ECA is electro-coagulated at 6 ⁇ 2 mm from its bifurcation from CCA.
  • a nylon thread (0.18 mm diameter) with the extremity coated with translucent hot melt adhesive constitutes the embolus (3 mm length, 0.36-0.38 mm diameter).
  • the embolus is inserted through a small incision into the ECA and is gently advanced into the ICA, until the cerebral blood flow decreases by 30 - 50% or a slight resistance is observed.
  • the neck incision is sutured.
  • the laser Doppler probe and the rectal temperature probe are removed.
  • the rats recover from anaesthesia and are placed back in their home cages.
  • Rats receive an intraperitoneal (i.p.) administration of physiological saline (1 ml/day), during 5 days to prevent dehydration.
  • IMD-026259 will be evaluated at three doses (1 , 10 and 100 ⁇ g/kg), Lv., 0, 2, 4 and 24 hours after reperfusion and compared to a vehicle control.
  • the experiment will include a sham control group administered with the vehicle under the same experimental conditions. The experiment will therefore include 5 groups. 12 rats are studied per group. The test is performed blind.
  • the Neurological Score is assessed according to a modified version of the method of Bederson and al. (Stroke, 1986, 17(3): 472-476).
  • test consists of 14 subsets as described below (table 1 ):
  • the test is performed 24 h and 48h after surgery.
  • the sections are scanned and infarct volumes are determined using ImageJ software (http://rsb.info.nih.gov/ij/).
  • the volumes of infarct are corrected relative to the volume of the whole brain and of the oedema (difference between the volume of the ipsi-and contralateral hemispheres).
  • Quantitative data obtained in the neurological score will be analyzed by a 2 way ANOVA (time x treatment) followed by 1 way ANOVA (treatment) and post-hoc comparisons using unpaired Student's t test.
  • data will be analyzed by comparing treated groups with an appropriate control group using unpaired Student's t tests.
  • Quantitative data obtained after infarct assessment will be analyzed by comparing treated groups with an appropriate control group using unpaired Student's t tests.
  • Example 15 Pulmonary hypertension (ex vivo) (prophetic).
  • Lungs from anasthesized mice are extracted from thorax by surgery.
  • the lungs are perfused und ventilated under isolation.
  • a Krebs-Henseleit buffer is used for perfusion.
  • the lungs are ventilated with a specific gas mixture (21%O2, 5,3% CO2).
  • Hypoxic vasoconstriction is induced by repeated hypoxic ventilation (1% O2, 5,3% CO2).
  • the periods of hypoxic ventilation last for 10 min each, while iterated with phases of normoxic ventilation for 15 min each.
  • Inhibitors (test items) are introduced into the perfusion media 5 min after the end of normoxic ventilation. Their effect on the strength of the HPV is quantified.
  • the HPV strength can be measured and indicated as an increase of pulmonary pressure (PAP; see figure 16 from Weissmann et al., Respir Physiol. 1995).
  • PAP pulmonary pressure
  • the PAP is directly proportional to the vascular resistance, since the lungs are perfused with constant volume (Roth et al. Am J Respir Crit Care Med 2009, in press; Weissmann et al., Proc Natl Acad Sci U S A. 2006 103:19093)
  • Example 16 Pulmonary hypertension (in vivo) (prophetic). To determine the effect of IMD-026259 on hypoxia-induced pulmonary hypertension mice are kept under chronical hypoxia (10% O 2 , normobar). Thereby, a pulmonary hypertension is developed within 3 weeks (Mittal et al., Circ Res. 2007 101 :258; Circulation. 2008 118:1183).
  • the pulmonary hypertension within this model is quantified by determination of cardiac hypertrophy, by quantification of right-ventricular systolic pressure and vascular morphometrie.
  • IMD-026259 is performed during hypoxia for three weeks twice daily via oral gavage at two different doses (1 und 3 mg/kg respectively).
  • IMD-026259 is capable to reduce or inhibit pulmonary hypertension, in particular whether by inhibition of HIF-1 the development of hypoxia-induced pulmonary hypertension can be suppressed.

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  • Pain & Pain Management (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Furan Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne des dérivés de cyclopentabenzofurane destinés au traitement et/ou à la prophylaxie d’affections liées à l’angiogenèse.
PCT/EP2009/008632 2008-12-05 2009-12-03 Inhibiteurs de l’accumulation de la protéine hif-1 WO2010063471A1 (fr)

Priority Applications (10)

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JP2011538897A JP2012510964A (ja) 2008-12-05 2009-12-03 Ｈｉｆ−１蛋白蓄積の阻害剤
CA2744391A CA2744391A1 (fr) 2008-12-05 2009-12-03 Inhibiteurs de l'accumulation de la proteine hif-1
AU2009321723A AU2009321723A1 (en) 2008-12-05 2009-12-03 Inhibitors of HIF-1 protein accumulation
MX2011005482A MX2011005482A (es) 2008-12-05 2009-12-03 Inhibidores de acumulacion de la proteina del factor 1 inducible por hipoxia.
BRPI0922938A BRPI0922938A2 (pt) 2008-12-05 2009-12-03 inibidores de acúmulo de proteína hif-1
CN2009801557467A CN102300568A (zh) 2008-12-05 2009-12-03 Hif-1蛋白积聚的抑制剂
EP09763878A EP2373311A1 (fr) 2008-12-05 2009-12-03 Inhibiteurs de l accumulation de la protéine hif-1
SG2011036688A SG171779A1 (en) 2008-12-05 2009-12-03 Inhibitors of hif-1 protein accumulation
IL213007A IL213007A0 (en) 2008-12-05 2011-05-19 Inhibitors of hif-1 protein accumulation
US13/152,365 US20120040956A1 (en) 2008-12-05 2011-06-03 Inhibitors of hif-1 protein accumulation

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US12010208P 2008-12-05 2008-12-05
US61/120,102 2008-12-05
EP08021162 2008-12-05
EP08021162.6 2008-12-05

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JP (1) JP2012510964A (fr)
KR (1) KR20110096566A (fr)
CN (1) CN102300568A (fr)
AU (1) AU2009321723A1 (fr)
BR (1) BRPI0922938A2 (fr)
CA (1) CA2744391A1 (fr)
IL (1) IL213007A0 (fr)
MX (1) MX2011005482A (fr)
SG (1) SG171779A1 (fr)
WO (1) WO2010063471A1 (fr)

Cited By (3)

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WO2012082765A2 (fr) 2010-12-16 2012-06-21 The United State Of America. As Represented By The Secretary Department Of Health And Human Services Méthodes pour réduire le poids corporel et traiter le diabète
WO2018160772A1 (fr) 2017-02-28 2018-09-07 The United State Of America, As Represented By The Secretary, Department Of Health & Human Services Procédé de traitement de l'obésité, de la résistance à l'insuline, d'une stéatose hépatique non alcoolique comprenant une stéatohépatite non alcoolique
US10519125B2 (en) 2014-07-04 2019-12-31 Pierre Fabre Medicament Flavagline derivatives

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JP6179904B2 (ja) * 2012-11-05 2017-08-16 国立大学法人山口大学 ソラフェニブの副作用低減剤
WO2014124006A1 (fr) 2013-02-05 2014-08-14 The Johns Hopkins University Nanoparticules pour le suivi de l'imagerie par résonance magnétique et procédés de fabrication et d'utilisation associés
WO2015120350A2 (fr) 2014-02-07 2015-08-13 Effector Therapeutics, Inc. Compositions et méthodes pour traiter des maladies fibrosantes
JP2018503736A (ja) * 2015-01-20 2018-02-08 ザ ジョーンズ ホプキンズ ユニバーシティＴｈｅ Ｊｏｈｎｓ Ｈｏｐｋｉｎｓ Ｕｎｉｖｅｒｓｉｔｙ 眼内圧を抑える抗緑内障剤の持続放出のための組成物
JP6846351B2 (ja) 2015-01-27 2021-03-24 ザ・ジョンズ・ホプキンス・ユニバーシティー 粘膜表面における活性薬剤の増強された輸送のための低張ヒドロゲル製剤
CN107427500A (zh) * 2015-03-20 2017-12-01 佐治亚州立大学研究基金会公司 治疗copd和其它炎症性病况的组合物和方法
CN105085450A (zh) * 2015-09-14 2015-11-25 中国药科大学 苯并呋喃类衍生物、其制备方法及其治疗作用

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WO2001012592A2 (fr) * 1999-08-18 2001-02-22 Warner-Lambert Company Composes d'acide hydroxamique utiles comme inhibiteurs de metalloproteinases matricielles
EP1693059A1 (fr) * 2005-02-22 2006-08-23 Deutsches Krebsforschungszentrum Utilisation des dérivés de rocaglamide en tant qu'inhibiteurs NF-À-spécifiques pour le traitement de certaines maladies inflammatoires
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012082765A2 (fr) 2010-12-16 2012-06-21 The United State Of America. As Represented By The Secretary Department Of Health And Human Services Méthodes pour réduire le poids corporel et traiter le diabète
US10519125B2 (en) 2014-07-04 2019-12-31 Pierre Fabre Medicament Flavagline derivatives
WO2018160772A1 (fr) 2017-02-28 2018-09-07 The United State Of America, As Represented By The Secretary, Department Of Health & Human Services Procédé de traitement de l'obésité, de la résistance à l'insuline, d'une stéatose hépatique non alcoolique comprenant une stéatohépatite non alcoolique

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MX2011005482A (es) 2011-06-16
CA2744391A1 (fr) 2010-06-10
US20120040956A1 (en) 2012-02-16
KR20110096566A (ko) 2011-08-30
CN102300568A (zh) 2011-12-28
AU2009321723A1 (en) 2010-06-10
JP2012510964A (ja) 2012-05-17
BRPI0922938A2 (pt) 2017-06-06
SG171779A1 (en) 2011-07-28
IL213007A0 (en) 2011-07-31
EP2373311A1 (fr) 2011-10-12

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