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  • A61K31/50—Pyridazines; Hydrogenated pyridazines
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  • A61P9/00—Drugs for disorders of the cardiovascular system
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• • A—HUMAN NECESSITIES
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  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
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  • C07D403/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
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  • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
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  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
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  • C07D471/12—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains three hetero rings
  • C07D471/14—Ortho-condensed systems

Definitions

• This invention is directed to polycyclic heteroaryl substituted triazoles and pharmaceutical compositions thereof which are useful as inhibitors of the receptor protein tyrosine kinase known as AxI.
• This invention is also directed to methods of using the compounds and compositions in treating diseases and conditions associated with AxI activity, particularly in treating diseases and conditions associated with angiogenesis and/or cell proliferation.
• AxI (also known as UFO, ARK, and Tyro7; nucleotide accession numbers NM_021913 and NM_001699; protein accession numbers NP_068713 and NP_001690) is a receptor protein tyrosine kinase (RTK) that comprises a C-terminal extracellular ligand-binding domain and N-terminal cytoplasmic region containing the catalytic domain.
• RTK receptor protein tyrosine kinase
• the extracellular domain of AxI has a unique structure that juxtaposes immunoglobulin and fibronectin Type III repeats and is reminiscent of the structure of neural cell adhesion molecules.
• Gas6 growth arrest specific-6
• Protein S Protein S.
• the AxI extracellular domain has been shown to undergo homophilic interactions that mediate cell aggregation, suggesting that one important function of AxI may be to mediate cell-cell adhesion.
• Ax! is predominantly expressed in the vasculature in both endothelial cells (EC's) and vascular smooth muscle cells (VSMC's) and in cells of the myeloid lineage and is also detected in breast epithelial cells, chondrocytes, Sertoli cells and neurons.
• EC's endothelial cells
• VSMC's vascular smooth muscle cells
• chondrocytes chondrocytes
• Sertoli cells and neurons Several functions including protection from apoptosis induced by serum starvation, TNF- ⁇ or the viral protein E1 A, as well as migration and cell differentiation have been ascribed to AxI signaling in cell culture.
• AxI-/- mice exhibit no overt developmental phenotype and the physiological function of AxI in vivo is not clearly established in the literature.
• Angiogenesis (the formation of new blood vessels) is limited to functions such as wound healing and the female reproductive cycle in healthy adults. This physiological process has been co-opted by tumors, thus securing an adequate blood supply that feeds tumor growth and facilitates metastasis.
• Deregulated angiogenesis also a feature of many other diseases (for example, psoriasis, rheumatoid arthritis, endometriosis and blindness due to age-related macular degeneration (AMD), retinopathy of prematurity and diabetes) and often contributes to the progression or pathology of the condition.
• AxI and/or its ligand has also been reported in a wide variety of solid tumor types including, but not limited to, breast, renal, endometrial, ovarian, thyroid, non-small cell lung carcinoma, and uveal melanoma as well as in myeloid leukemia's. Furthermore, it possesses transforming activity in NIH3T3 and 32D cells. It has been demonstrated that loss of AxI expression in tumor cells blocks the growth of solid human neoplasms in an in vivo MDA-MB-231 breast carcinoma xenograft model. Taken together, these data suggest AxI signaling can independently regulate EC angiogenesis and tumor growth and thus represents a novel target class for tumor therapeutic development.
• AxI and Gas6 proteins are upregulated in a variety of other disease states including endometriosis, vascular injury and kidney disease and AxI signaling is functionally implicated in the latter two indications.
• AxI - Gas6 signaling amplifies platelet responses and is implicated in thrombus formation.
• AxI may thus potentially represent a therapeutic target for a number of diverse pathological conditions including solid tumors, including, but not limited to, breast, renal, endometrial, ovarian, thyroid, non-small cell lung carcinoma and uveal melanoma; liquid tumors, including but not limited to, leukemias (particularly myeloid leukemias) and lymphomas; endometriosis, vascular disease / injury (including but not limited to restenosis, atherosclerosis and thrombosis), psoriasis; visual impairment due to macular degeneration; diabetic retinopathy and retinopathy of prematurity; kidney disease (including but not limited to glomerulonephritis, diabetic nephropathy and renal transplant rejection), rheumatoid arthritis; osteoporosis, osteoarthritis and cataracts.
• solid tumors including, but not limited to, breast, renal, endometrial, ovarian, thyroid, non-small cell lung carcinoma and uveal mel
• This invention is directed to certain polycyclic heteroaryl substituted triazoles which are useful as AxI inhibitors, methods of using such compounds in treating diseases and conditions associated with AxI activity and pharmaceutical compositions comprising such compounds.
• this invention is directed to compounds of formula (I):
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 or -N(R 2 )-R 4 -C(O)OR 3 ; or R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2 ; each R 2 and R 3 is hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and each R 4 is independently selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain; as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof; provided that the compound of formula (I) is not a compound selected from the group consisting of: 1-(6,7-dihydro-5/-/
• this invention is directed to compounds of formula (II):
• R 5 is ⁇ /-heterocyclyl optionally substituted by ⁇ /-heterocyclyl; and R 6 is h3lo;
• this invention is directed to pharmaceutical compositions comprising a pharmaceutically acceptable excipient and a compound of formula (I), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof, or a compound of formula (II), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• this invention is directed to methods of treating a disease or condition associated with AxI activity in a mammal, wherein the methods comprise administering to the mammal a therapeutically effective amount of a compound of formula (I), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof, or a therapeutically effective amount of a pharmaceutical composition comprising a pharmaceutically acceptable excipient and a compound of formula (I), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof, or a compound of formula (II), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof, or a therapeutically effective amount of a pharmaceutical composition comprising a pharmaceutically acceptable excipient and a compound of formula (II), as described above, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• this invention provides methods of preparing an (S)-enantiomer of the following formula:
• n and m are the same and are 0, 1 or 2;
• R 7 is nitro, halo or -C(O)OR 2 ;
• R 2 is hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroaryl; wherein the method comprises treating a compound of formula (i):
• n and m are the same and are 0, 1 or 2;
• R 7 is nitro, halo or -C(O)OR 2 ;
• R 2 is hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroaryl; with an amino donor molecule in the presence of a catalytic amount of a (S)-specific transaminase under suitable conditions to form the (S)-enantiomer.
• this invention provides assays to determine a compound of the invention effectiveness in inhibiting AxI activity in a cell-based assay.
• Carboxy refers to the -C(O)OH radical.
• Niro refers to the -NO 2 radical.
• Oxa refers to the -O- radical.
• Alkyl refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to twelve carbon atoms, preferably one to eight carbon atoms or one to six carbon atoms (“lower alkyl”), and which is attached to the rest of the molecule by a single bond, for example, methyl, ethyl, n-propyl, 1-methylethyl (/so-propyl), n-butyl, n-pentyl, 1 ,1-dimethylethyl (f-butyl), 3-methylhexyl, 2-methylhexyl, and the like.
• Alkenyl refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing at least one double bond, having from two to twelve carbon atoms, preferably one to eight carbon atoms and which is attached to the rest of the molecule by a single bond, for example, ethenyl, prop-1-enyl, but-1-enyl, pent-1-enyi, penta-1 ,4-dienyl, and the like.
• Alkylene or "alkylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing no unsaturation and having from one to twelve carbon atoms, for example, methylene, ethylene, propylene, n-butylene, and the like.
• the alkylene chain is attached to the rest of the molecule through a single bond and to the radical group through a single bond.
• the points of attachment of the alkylene chain to the rest of the molecule and to the radical group can be through one carbon in the alkylene chain or through any two carbons within the chain.
• amino-donor molecule refers to a organic molecule comprising a primary amine (-NH 2 ) group and which is suitable for the processes disclosed herein.
• amino-donor molecules include, but are not limited to, ⁇ -amino acids, such as alanine and phenylalanine, isopropylamine, 1-ethylpropylamine, 1 ,1 ,3,3-tetramethylbutylamine, 1 ,2-dimethylbutylamine, sec-butylamine, 1-phenylethylamine and the like.
• Aryl refers to a hydrocarbon ring system radical comprising hydrogen, 6 to 14 carbon atoms and at least one aromatic ring.
• the aryl radical may be a monocyclic, bicyclic, or tricyclic system and which may include spiro ring systems.
• An aryl radical is commonly, but not necessarily, attached to the parent molecule via an aromatic ring of the aryl radical.
• Alkyl refers to a radical of the formula -R b -R 0 where R b is an alkylene chain as defined above and R c is one or more aryl radicals as defined above, for example, benzyl, diphenylmethyl and the like.
• Cycloalkyl refers to a stable non-aromatic monocyclic or polycyclic hydrocarbon radical consisting solely of carbon and hydrogen atoms, which may include spiro or bridged ring systems, having from three to fifteen carbon atoms, preferably having from three to ten carbon atoms, more preferably from five to seven carbons and which is saturated or unsaturated and attached to the rest of the molecule by a single bond.
• a bridged ring system is a system wherein two non-adjacent ring atoms thereof are connected through an atom or a group of atoms.
• Monocyclic cycloalkyl radicals include non-bridged cycloalkyl radicals, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
• Polycyclic radicals include fused, spiro or bridged cycloalkyl radicals, for example, C 10 radicals such as adamantanyl (bridged) and decalinyl (fused), and C 7 radicals such as bicyclo[3.2.0]heptanyl (fused), norbornanyl and norbomenyl (bridged), as well as substituted polycyclic radicals, for example, substituted C 7 radicals such as bicyclo[2.2.1]heptenyl (bridged) and 7,7-dimethylbicyclo[2.2.1]heptanyl (bridged), and the like.
• Cycloalkylalkyl refers to a radical of the formula -R b R 9 where R b is an alkylene chain as defined above and R 9 is a cycloalkyl radical as defined above.
• Enantiomeric excess refers to a product wherein one enantiomer is present in excess of the other, and is defined as the absolute difference in the mole fraction of each enantiomer. Enantiomeric excess is typically expressed as a percentage of an enantiomer present in a mixture relative to the other enantiomer.
• the (S)-enantiomer of the invention is considered to be substantially free of the (R)-enantiomer when the (S)-enantiomer is present in enantiomeric excess of greater than 80%, preferably greater than 90%, more preferably greater than 95% and most preferably greater than 99%.
• Halo refers to bromo, chloro, fluoro or iodo.
• Haloalkyl refers to an alkyl radical, as defined above, that is substituted by one or more halo radicals, as defined above, for example, trifluoromethyl, difluoromethyl, trichloromethyl, 2,2,2-trifluoroethyl, 1-fluoromethyl-2-fluoroethyl, 3-bromo-2-fluoropropyl, 1-bromomethyl-2-bromoethyl, and the like.
• Heterocyclyl refers to a stable 3- to 18-membered non-aromatic ring radical which comprises one to twelve carbon atoms and from one to six heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur.
• the heterocyclyl radical may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include spiro or bridged ring systems; and the nitrogen, carbon or sulfur atoms in the heterocyclyl radical may be optionally oxidized; the nitrogen atom may be optionally quatemized; and the heterocyclyl radical may be partially or fully saturated.
• heterocyclyl radicals include, but are not limited to, dioxolanyl, 1 ,4-diazepanyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, octahydro-1 H-pyrroIo[3,2-c]pyridinyl, octahydro-1 /-/-pyrrolo[2,3- cjpyridinyl, octahydro-1 /-/-pyrrolo[2,3-jb]pyridinyl, octahydro-1 H-pyrrolo[3,4- ⁇ ]pyridinyl, octahydropyrrolo[3,4-c]pyrrolyl, octahydro-1 H-pyr
• ⁇ /-heterocyclyl refers to a heterocyclyl radical as defined above containing at least one nitrogen and where the point of attachment of the heterocyclyl radical to the rest of the molecule is through a nitrogen atom in the heterocyclyl radical.
• An ⁇ /-heterocyclyl radical may be optionally substituted as described above for heterocyclyl radicals.
• Heteroaryl refers to a 5- to 14-membered ring system radical comprising hydrogen atoms, one to thirteen carbon atoms, one to six heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur, and at least one aromatic ring.
• a heteroaryl radical is commonly, but not necessarily, attached to the parent molecule via an aromatic ring of the heteroaryl radical.
• the heteroaryl radical may be a monocyclic, bicyclic or tricyclic ring system, which may include spiro ring systems; and the nitrogen, carbon or sulfur atoms in the heteroaryl radical may be optionally oxidized; the nitrogen atom may be optionally quaternized.
• the aromatic ring of the heteroaryl radical need not contain a heteroatom, as long as one ring of the heteroaryl radical contains a heteroatom.
• 1 ,2,3,4-tetrahydroisoquinolin-7-yl is considered a "heteroaryl" for the purposes of this invention.
• heteroaryl radical as defined herein can not contain rings having more than 7 members and cannot contain rings wherein two non-adjacent members thereof are connected through an atom or a group of atoms (i.e., a bridged ring system).
• heteroaryl radicals include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1 ,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzothiazolyl, benzothiadiazolyl, benzo[£>][1 ,4]dioxepinyl, benzo[jb][1 ,4]oxazinyl, 1 ,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl), benzothieno[3,2-c(]pyrimidinyl, benzotriazolyl, benzo[4,6]imi
• Keto acid refers to a carboxylic acid additionally containing a ketone functional group.
• An “2-keto acid” refers to a carboxylic acid wherein the ketone functional group is adjacent to the carboxylic acid (-C(O)OH) group.
• C 7 -C 12 alkyl describes an alkyl group, as defined below, having a total of 7 to 12 carbon atoms
• C 4 -C 12 cycloalkylalkyl describes a cycloalkylalkyl group, as defined below, having a total of 4 to 12 carbon atoms.
• the total number of carbons in the shorthand notation does not include carbons that may exist in substituents of the group described.
• Solid compound and “stable structure” are meant to indicate a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.
• “Mammal” includes humans and domestic animals, such as cats, dogs, swine, cattle, sheep, goats, horses, rabbits, and the like. Preferably, for purposes of this invention, the mammal is a human.
• Optional or “optionally” means that the subsequently described event of circumstances may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not.
• optionally substituted aryl means that the aryl radical may or may not be substituted and that the description includes both substituted aryl radicals and aryl radicals having no substitution.
• “Pharmaceutically acceptable excipient” includes without limitation any adjuvant, carrier, excipient, glidant, sweetening agent, diluent, preservative, dye/colorant, flavor enhancer, surfactant, wetting agent, dispersing agent, suspending agent, stabilizer, isotonic agent, solvent, or emulsifier which has been approved by the United States Food and Drug Administration as being acceptable for use in humans or domestic animals.
• “Pharmaceutically acceptable salt” includes both acid and base addition salts.
• “Pharmaceutically acceptable acid addition salt” refers to those salts which retain the biological effectiveness and properties of the free bases, which are not biologically or otherwise undesirable, and which are formed with inorganic acids such as, but not limited to, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and organic acids such as, but not limited to, acetic acid, 2,2-dichloroacetic acid, adipic acid, alginic acid, ascorbic acid, aspartic acid, benzenesulfonic acid, benzoic acid, 4-acetamidobenzoic acid, camphoric acid, camphor-10-sulfonic acid, capric acid, caproic acid, caprylic acid, carbonic acid, cinnamic acid, citric acid, cyclamic acid, dodecylsulfonic acid, ethane-1 ,2-disulfonic acid
• “Pharmaceutically acceptable base addition salt” refers to those salts which retain the biological effectiveness and properties of the free acids, which are not biologically or otherwise undesirable. These salts are prepared from addition of an inorganic base or an organic base to the free acid. Salts derived from inorganic bases include, but are not limited to, the sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like. Preferred inorganic salts are the ammonium, sodium, potassium, calcium, and magnesium salts.
• Salts derived from organic bases include, but are not limited to, salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as ammonia, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, diethanolamine, ethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, benethamine, benzathine, ethylenediamine, glucosamine, methylglucamine, theobromine, triethanolamine, tromethamine, purines, piperazine, piperidine, ⁇ /-ethylpiperidine, polyamine resins and the like.
• Particularly preferred organic bases are isopropylamine
• a “pharmaceutical composition” refers to a formulation of a compound of the invention and a medium generally accepted in the art for the delivery of the biologically active compound to mammals, for example, humans.
• a medium includes all pharmaceutically acceptable carriers, diluents or excipients therefor.
• “Therapeutically effective amount” refers to that amount of a compound of the invention which, when administered to a mammal, preferably a human, is sufficient to effect treatment, as defined below, of a disease or condition of interest in the mammal, preferably a human.
• the amount of a compound of the invention which constitutes a “therapeutically effective amount” will vary depending on the compound, the disease or condition and its severity, and the age of the mammal to be treated, but can be determined routinely by one of ordinary skill in the art having regard to his own knowledge and to this disclosure.
• “Treating” or “treatment” as used herein covers the treatment of the disease or condition of interest in a mammal, preferably a human, having the disease or condition of interest, and includes:
• Transaminases refers to naturally occurring or non-natural enzymes which catalyze the transfer of an amino group from an amino donor molecule to a ketone-containing molecule, preferably to a cyclic ketone fused to an aromatic ring, to produce an optically active molecule.
• Transaminases, or aminotransferases have high stereoselectivity for a given enantiomer.
• the process of transamination utilizing a transaminase is a chiral synthesis, not a resolution.
• the terms “disease” and “condition” may be used interchangeably or may be different in that the particular malady or condition may not have a known causative agent (so that etiology has not yet been worked out) and it is therefore not yet recognized as a disease but only as an undesirable condition or syndrome, wherein a more or less specific set of symptoms have been identified by clinicians.
• the compounds of the invention, or their pharmaceutically acceptable salts may contain one or more asymmetric centres and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)- or, as (D)- or (L)- for amino acids.
• the present invention is meant to include all such possible isomers, as well as their racemic and optically pure forms.
• Optically active (+) and (-), (R)- and (S)-, or (D)- and (L)- isomers may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques, such as HPLC using a chiral column.
• the compounds described herein contain olefinic double bonds or other centres of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers. Likewise, all tautomeric forms are also intended to be included.
• stereoisomer refers to a compound made up of the same atoms bonded by the same bonds but having different three-dimensional structures, which are not interchangeable.
• the present invention contemplates various stereoisomers and mixtures thereof and includes “enantiomers”, which refers to two stereoisomers whose molecules are nonsuperimposeable mirror images of one another.
• a "tautomer” refers to a proton shift from one atom of a molecule to another atom of the same molecule.
• the present invention includes tautomers of any said compounds.
• the chemical naming protocol and structure diagrams used herein are a modified form of the I.U.P.A.C. nomenclature system wherein the compounds of the invention are named herein as derivatives of the central core structure, i.e., the triazole structure.
• a substituent group is named before the group to which it attaches.
• cyclopropylethyl comprises an ethyl backbone with cyclopropyl substituent.
• all bonds are identified, except for some carbon atoms, which are assumed to be bonded to sufficient hydrogen atoms to complete the valency.
• a compound of formula (II), as set forth above in the Summary of the Invention, where R 5 is 4-(4-(pyrrolidin-1-yl)piperidin-1-yl) and R 6 is 3-fluoro is named herein as 1-(6,7-dihydro-5/-/-pyrido[2',3':6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -(3-fluoro- 4-(4-(pyrrolidin-1-yl)piperidin-1-yl)phenyl)-1/-/-1 ,2,4-triazole-3,5-diamine.
• One embodiment of the invention is a compound of formula (I), as set forth above in the Summary of the Invention, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 or -N(R 2 )-R 4 -C(O)OR 3 ; or
• R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2 ;
• each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl;
• each R 4 is independently selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• a compound of formula (Ia), as set forth above is a compound of formula (Ia1 )
• R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl.
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, wherein R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl and alkenyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, selected from the group consisting of: 1-(6,7-dihydro-5H-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -((7S)-7-amino-6,7,8,9- tetrahydro-5H-benzo[7]annulene-2-yi)-1H-1 ,2,4-triazole-3,5-diamine; 1-(6,7-dihydro-5/-/-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -((7S)-7-((2- methylpropyl)amino)-6,7,8,9-tetrahydro-5/-/-benzo[7]annulene-2-yl)-1/-/-1 ,2,4- triazole-3,5-d
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, wherein R 2 and R 3 are each independently selected from the group consisting of hydrogen, optionally substituted cycloalkyl and optionally substituted cycloalkylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, selected from the group consisting of: 1-(6,7-dihydro-5H-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -((7s)-7-
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, wherein R 2 and R 3 are each independently selected from the group consisting of hydrogen, optionally substituted aralkyl and optionally substituted heteroarylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia1 ), as set forth above, selected from the group consisting of:
• R 1 is -N(R 2 )C(O)R 3 ; and R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• a compound of formula (Ia) is a compound of formula (Ia2):
• R 2 and R 3 are each independently selected from the group consisting of hydrogen and alkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia2), as set forth above, which is 1-(6,7-dihydro-5H-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -(7-
• R 1 is -N(R 2 )-R 4 -C(O)OR 3 ;
• R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and R 4 is selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is where a compound of formula (Ia), as set forth above, is a compound of formula (Ia3):
• R 2 and R 3 are each independently selected from the group consisting of hydrogen and alkyl
• R 4 is selected from the group consisting of a direct bond and a methylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia3), as set forth above, selected from the group consisting of: 1-(6,7-dihydro-5H-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)-A/ 3 -(7- ((methoxycarbonylmethyl)(methyl)amino)-6,7,8,9-tetrahydro-5H- benzo[7Jannulene-2-yl)-1 H- 1 ,2,4-triazole-3,5-diamine; 1-(6,7-dihydro-5/-/-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -(7-
• R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2 ;
• R 2 is selected from the group consisting of hydrogen, alky], alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 2 is selected from the group consisting of hydrogen and alkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia), as set forth above, selected from the group consisting of: 1-(6,7-dihydro-5H-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -(7-((2/?)-2-
• R 1 is -N(R 2 )-R 4 -C(O)OR 3 , each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and each R 4 is independently selected from the group consisting of a direct bond or an optionally substituted straight or branched alkylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is where a compound of formula (Ia), as set forth above, is a compound of formula (Ia4):
• R 2 and R 3 are each independently selected from the group consisting of hydrogen and alkyl
• R 4 is a direct bond or a methylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia3), as set forth above, which is 1-(6,7-dihydro-5H-pyrido[2' I 3 l :6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 5 - ((7S)-7-(f-butoxycarbonylamino)-6,7,8,9-tetrahydro-5/-/-benzo[7]annulene-2-yl)-1/-/-1 ,2,4- triazole-3,5-diamine, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is where a compound of formula (Ia), as set forth above, is a compound of formula (Ia5):
• R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl, cycloalkyl and cycloalkylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ia5), as set forth above, selected from the group consisting of: 1-(6,7-dihydro-5H-pyrido[2',3':6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 3 -((7S)-7-amino-
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 or -N(R 2 )-R 4 -C(O)OR 3 ; or R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2 ; each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and each R 4 is independently selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain; and as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 or -N(R 2 )-R 4 -C(O)OR 3 ; or R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2 ; each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and each R 4 is independently selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 1 is -N(R 2 )-R 4 -C(O)OR 3 ;
• R 2 and R 3 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl; and R 4 is selected from the group consisting of a direct bond and an optionally substituted straight or branched alkylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is where a compound of formula (Ib), as set forth above, is a compound of formula (Ib1 ): wherein:
• R 2 and R 3 are each independently selected from the group consisting of hydrogen and alkyl; and R 4 is selected from the group consisting of a direct bond and a methylene chain, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ib1 ), as set forth above, which is 1-(6,7-dihydro-5/-/-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 5 -((7S)-7- (Nbutoxycarbonylamino)-6,7,8,9-tetrahydro-5/-/-benzo[7]annulene-2-yl)-1 /-/-1 ,2,4-triazole- 3,5-diamine, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 2 is selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroarylalkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• R 2 is selected from the group consisting of hydrogen and alkyl, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (Ib), as set forth above, which is 1-(6,7-dihydro-5/-/-benzo[6,7]cyclohepta[1 ,2-c]pyridazin-3-yl)- ⁇ / 5 -(7-
• Another embodiment of the invention is a compound of formula (II), as set forth above in the Summary of the Invention, wherein: R 5 is piperidinyl substituted by pyrrolidinyl; and
• R 6 is halo, as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Another embodiment of the invention is a compound of formula (II), as set forth above in the Summary of the Invention, which is 1-(6,7-dihydro-5H-
• Another embodiment of the invention is a method of preparing an (S)-enantiomer of the following formula:
• Another embodiment of the invention is a method of preparing an (S)-enantiomer of the following formula: er . as set forth above in the Summary of the Invention, wherein the compound of formula (i) is a compound of formula (Cb-1 ):
• Another embodiment of the invention is a method of preparing an (S)-enantiomer of the following formula:
• compositions of the invention comprising a pharmaceutically acceptable excipient and a compound of the invention, as set forth above in the Summary of the Invention, certain embodiments are preferred.
• One embodiment of these pharmaceutical compositions is wherein the compound of the invention therein is selected from any one embodiment of the compounds of the invention, as set forth above, or from any combination of embodiments of the compounds of the inventioin, as set forth above.
• methods of treating a disease or condition associated with AxI activity in a mammal wherein the method comprises administering to a mammal in need thereof a therapeutically effective amount of a compound the invention, certain embodiments are preferred.
• One embodiment of these methods is the method wherein the disease or condition is selected from the group consisting of rheumatoid arthritis, vascular disease, vascular injury, psoriasis, visual impairment due to macular degeneration, diabetic retinopathy, retinopathy of prematurity, kidney disease, osteoarthritis and cataracts.
• One embodiment of these methods is the method wherein a manifestation of the disease or condition is solid tumor formation in said mammal.
• a manifestation of the disease or condition is solid tumor formation in said mammal.
• the disease or condition is selected from the group consisting of breast carcinoma, renal carcinoma, endometrial carcinoma, ovarian carcinoma, thyroid carcinoma, non-small cell lung carcinoma, and uveal melanoma.
• One embodiment of these methods is the method wherein a manifestation of the disease or condition is liquid tumor formation in said mammal.
• One embodiment of these methods is the method wherein the disease or condition is myeloid leukemia or lymphoma.
• One embodiment of these methods is the method wherein the disease or condition is endometriosis.
• One embodiment of these methods is the method wherein the disease or condition is metastasis to the liver.
• One embodiment of these methods is the method wherein the compounds of invention utilized therein is selected from any one embodiment of the compounds of the invention, as set forth above, or from any combination of embodiments of the compounds of the invention, as set forth above.
• Another embodiment of the invention are those methods of treating a disease or condition associated with AxI activity by administering to the mammal a therapeutically effective amount of a pharmaceutical composition of the invention, as set forth above in the Summary of the Invention, wherein the disease or condition is selected from the group consisting of rheumatoid arthritis, vascular disease / injury (including but not limited to restenosis, atherosclerosis and thrombosis), psoriasis, visual impairment due to macular degeneration, diabetic retinopathy or retinopathy of prematurity, kidney disease (including but not limited to glomerulonephritis, diabetic nephropathy and renal transplant rejection), osteoarthritis and cataracts.
• vascular disease / injury including but not limited to restenosis, atherosclerosis and thrombosis
• psoriasis psoriasis
• visual impairment due to macular degeneration diabetic retinopathy or retinopathy of prematurity
• kidney disease including but not
• Another embodiment of the invention are those methods of treating a disease or condition associated with AxI activity by administering to the mammal a therapeutically effective amount of a pharmaceutical composition of the invention, as set forth above in the Summary of the Invention, wherein the disease or condition is selected from the group consisting of breast carcinoma, renal carcinoma, endometrial carcinoma, ovarian carcinoma, thyroid carcinoma, non-small cell lung carcinoma, uveal melanoma, myeloid leukemia and lymphoma.
• Another embodiment of the invention are those methods of treating a disease or condition associated with AxI activity by administering to the mammal of therapeutically effective amount of a pharmaceutical composition of the invention, as set forth above in the Summary of the Invention, wherein the disease or condition is endometriosis. Specific embodiments of the invention are described in more detail in the following sections.
• the oncogenic RTK, AxI was recently identified, using a retroviral-based functional genetic screening protocol, as a regulator of haptotactic migration, which is a key event in angiogenesis.
• AxI inhibition by RNAi-mediated silencing blocked endothelial cell migration, proliferation and in vitro tube formation.
• AxI signaling therefore, impacts multiple functions required for neovascularization in vitro, and regulates angiogenesis in vivo. Regulation of these pro-angiogenic processes required the catalytic activity of AxI. Thus, Axl-mediated angiogenic stimulation would be amenable to modulation by a small molecule inhibitor of AxI catalytic activity.
• the compounds of the invention are small molecule inhibtiors of AxI catalytic activity, and are therefore useful in treating diseases and conditions which are associated with AxI catalytic activity including those diseases and conditions which are characterized by angiogenesis and/or cell proliferation.
• the compounds of the invention and pharmaceutical compositions of the invention are useful in treating diseases and conditions which are alleviated by the modulation of AxI activity.
• modulation of AxI activity includes diseases and conditions which are alleviated by a decrease in AxI activity and diseases and conditions which are alleviated by an increase in AxI activity. Preferably such diseases and conditions are alleviated by a decrease in AxI activity.
• Diseases and conditions which are alleviated by the modulation of AxI activity include, but are not limited to, solid tumors, including, but not limited to, breast, renal, endometrial, ovarian, thyroid, and non-small cell lung carcinoma, melanoma, prostate carcinoma, sarcoma, gastric cancer and uveal melanoma; liquid tumors, including but not limited to, leukemias (particularly myeloid leukemias) and lymphomas; endometriosis, vascular disease / injury (including but not limited to restenosis, atherosclerosis and thrombosis), psoriasis; visual impairment due to macular degeneration; diabetic retinopathy and retinopathy of prematurity; kidney disease (including but not limited to glomerulonephritis, diabetic nephropathy and renal transplant rejection), rheumatoid arthritis; osteoarthritis, osteoporosis and cataracts.
• solid tumors including, but not limited to, breast, renal
• the compounds of the invention are useful in treating diseases and conditions which are affected by the following biological processes:
• the compounds of the invention may also be used to modulate inflammatory processes by treating sepsis, acting as vaccine adjuvants, and/or potentiating the immune response in immuno-compromised patients. In one embodiment, the compounds of the invention are effective in treating metastasis to the liver.
• treatment with compounds of the invention can result in pronounced reduction in the development of liver micrometastases.
• One method of the invention is treatment of a patient with compounds of the invention to reduce metastasis to the liver. This method can be done with compounds of the invention alone or in combination with other agents to produce the desired therapeutic benefit.
• a cell proliferative disorder refers to a disorder characterized by abnormal proliferation of ceils.
• a proliferative disorder does not imply any limitation with respect to the rate of cell growth, but merely indicates loss of normal controls that affect growth and cell division. Thus, in some embodiments, cells of a proliferative disorder can have the same cell division rates as normal cells but do not respond to signals that limit such growth.
• neoplasm or tumor which is an abnormal growth of tissue. Cancer refers to any of various malignant neoplasms characterized by the proliferation of cells that have the capability to invade surrounding tissue and/or metastasize to new colonization sites.
• cell proliferative disorders treatable with the compounds of the invention relate to any disorder characterized by aberrant cell proliferation. These include various tumors and cancers, benign or malignant, metastatic or non-metastatic. Specific properties of cancers, such as tissue invasiveness or metastasis, can be targeted using the methods described herein.
• Cell proliferative disorders include a variety of cancers, including, among others, breast cancer, ovarian cancer, renal cancer, gastrointestinal cancer, kidney cancer, bladder cancer, pancreatic cancer, lung squamous carcinoma, and adenocarcinoma.
• the compounds of the invention are useful in treating renal cell carcinoma, clear cell carcinoma of kidney, and renal cell adenocarcinoma; invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma, lobular carcinoma in situ, and metastatic breast cancer; basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and Karposi's sarcoma; small cell and non-small cell lung carcinoma, bronchial adema, pleuropulmonary blastoma, and malignant mesothelioma; brain stem and hyptothalamic glioma, cerebellar and cerebral astrocytoma, medullablastoma, ependymal tumors, oligodendroglial, meningiomas, and neuroectodermal and pineal tumors; prostate cancer, testicular cancer, and penile cancer; uterine cancer (endometrial), cervical, ovarian, vaginal, vulval cancers
• the compounds of the invention are useful in treating precursor B-lymphoblastic leukemia/lymphoma (precursor B-cell acute lymphoblastic leukemia), B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma, B-cell prolymphocytic leukemia, lymphoplasmacytic lymphoma, splenic marginal zone B-cell lymphoma, hairy cell leukemia, plasma cell myeloma/plasmacytoma, extranodal marginal zone B-cell lymphoma of MALT type, nodal marginal zone B-cell lymphoma, follicular lymphoma, mantle-cell lymphoma, diffuse large B-cell lymphoma, mediastinal large B-cell lymphoma, primary effusion lymphoma, and Burkitt's lymphoma/Burkitt cell leukemia
• the compounds of the invention are useful in treating precursor T-lymphoblastic lymphoma/leukemia (precursor T-cell acute lymphoblastic leukemia), T-cell prolymphocytic leukemia T-cell granular lymphocytic leukemia, aggressive NK-cell leukemia, adult T-cell lymphoma/leukemia (HTLV-1 ), extranodal NK/T-cell lymphoma, nasal type, enteropathy-type T-cell lymphoma, hepatosplenic gamma-delta T-cell lymphoma, subcutaneous panniculitis-like T-cell lymphoma, mycosis fungoides/Sezary syndrome, anaplastic large-cell lymphoma, T/null cell, primary cutaneous type, peripheral T-cell lymphoma not otherwise characterized, angioimmunoblastic T-cell lymphoma, anaplastic large-cell lymphoma, T
• the compounds of the invention are useful in treating nodular lymphocyte-predominant Hodgkin's lymphoma, nodular sclerosis Hodgkin's lymphoma (grades 1 and 2), lymphocyte-rich classical Hodgkin's lymphoma, mixed cellularity Hodgkin's lymphoma, and lymphocyte depletion Hodgkin's lymphoma.
• the compounds of the invention are also useful in treating myelogenous leukemia (e.g., Philadelphia chromosome positive (t(9;22)(qq34;q11 )), chronic neutrophilic leukemia, chronic eosinophilic leukemia/hypereosinophilic syndrome, chronic idiopathic myelofibrosis, polycythemia vera, and essential thrombocythemia, chronic myelomonocytic leukemia, atypical chronic myelogenous leukemia, and juvenile myelomonocytic leukemia, refractory anemia (with ringed sideroblasts and without ringed sideroblasts), refractory cytopenia (myelodysplastic syndrome) with multilineage dysplasia, refractory anemia (myelodysplastic syndrome) with excess blasts, 5q-syndrome, and myelodysplastic syndrome with t(9;12)(q22;p12
• the compounds of the invention are also useful in treating acute myelogenous leukemia with t(8;21 )(q22;q22), AML1 (CBF-alpha)/ETO, acute promyelocytic leukemia (AML with t(15;17)(q22;q11-12) and variants, PML/RAR-alpha), acute myelogenous leukemia with abnormal bone marrow eosinophils (inv(16)(p13q22) or t(16;16)(p13;q11 ), CBFb/MYH11X), and acute myelogenous leukemia with 11q23 (MLL) abnormalities, acute myelogenous leukemia minimally differentiated, acute myelogenous leukemia without maturation, acute myelogenous leukemia with maturation, acute myelomonocytic leukemia, acute monocytic leukemia, acute erythroid leukemia, acute megakaryocytic leuk
• the compounds of the invention may be tested for their use in treating leukemias and lymphomas by testing the compounds in the xenograft in SCID mouse model using human Axl-expresing cancer cell lines including, but not limited to, HeLa, MDA-MB-231 , SK-OV-3, OVCAR-8, DU145, H1299, ACHN, A498 and Caki-1.
• the compounds of the invention may be tested for their use in treating leukemias in the xenograft in SCID or nu/nu mouse model using human Axl-expressing AML and CML leukemia cell lines.
• the compounds of the invention may be tested for their use in treating endometriosis by using the syngenic mouse model of endometriosis (see Somigiiana, E. et al., "Endometrial ability to implant in ectopic sites can be prevented by inter!eukin-12 in a murine model of endometriosis", Hum. Reprod. (1999), Vol. 14, NO. 12, pp. 2944-50).
• the compounds may also be tested for their use in treating endometriosis by using the rat model of endometriosis (see Lebovic, D.I.
• the compounds of the invention may be tested for their use in treating restenosis by using the balloon-injured rate carotid artery model (see Kim, D.W. et al., "Novel oral formulation of paclitaxel inhibits neointimal hyperplasia in a rat carotid artery injury model", Circulation (2004), Vol. 109, No. 12, pp. 1558-63, Epub 2004 Mar 8).
• the compounds of the invention may also be tested for their use in treating restenosis by using the percutaneous transluminal coronary angioplasty in apoE deficient mouse model (see von der Thusen, J. H. et al., "Adenoviral transfer of endothelial nitric oxide synthase attenuates lesion formation in a novel murine model of postangioplasty restenosis", Arterioscler. Thromb. Vase. Biol. (2004), Vol. 24, No. 2, pp. 357-62).
• the compounds of the invention may be tested for their use in treating atherosclerosis/thrombosis in the ApoE deficient mouse model (see Nakashima, Y. et al., "ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree", Arterioscler. Thromb. (1994), Vol. 14, No. 1 , pp. 133-40).
• the compounds of the invention may also be tested for their use in treating thrombosis using the collagen-epinephrin-induced pulmonary thromboembolism model and the stasis induced venous thrombosis model (see Angelillo-Scherrer A. et al., "Role of Gas6 receptors in platelet signaling during thrombus stabilization and implications for antithrombotic therapy", J Clin Invest. (2005) VoI 115 pp237-46).
• the compounds of the invention may be tested for their use in treating psoriasis by using the SCID mouse model or the human skin model of psoriasis (see Nickoloff, B.J. et ai, "Severe combined immunodeficiency mouse and human psoriatic skin chimeras. Validation of a new animal model", Am. J. Pathol. (1995), Vol. 146, No. 3, pp. 580-8).
• the compounds of the invention may be tested for their use in treating age- related macular degeneration or diabetic retinopathy by using the rat corneal angiogenesis model (see Sarayba MA, Li L, Tungsiripat T, Liu NH, Sweet PM, Patel AJ, Osann KE, Chittiboyina A, Benson SC, Pershadsingh HA, Chuck RS. Inhibition of corneal neovascularization by a peroxisome proliferator-activated receptor-gamma ligand. Exp Eye Res. 2005 Mar;80(3):435-42) or the laser-induced mouse choroidal neovasculation model (see Bora, P.
• the compounds of the invention may be tested for their use in treating retinopathy of prematurity in the mouse retinopathy of prematurity model (see Smith, L. E. et al., "Oxygen-induced retinopathy in the mouse", Invest. Ophthalmol. Vis. Sci. (1994), Vol. 35, No. 1 , pp. 101-11 ).
• the compounds of the invention may be tested for their use in treating glomerulonephritis or diabetic nephropathy in the rat anti-Thy1.1 -induced experimental mesengial proliferative glomerulonephritis model (see Smith, L.E. et al. cited above).
• the compounds of the invention may be tested for their use in treating renal tranplant rejection by using a rat model of chronic renal transplant rejection (see Yin, J. L. et al., "Expression of growth arrest-specific gene 6 and its receptors in a rat model of chronic renal transplant rejection", Transplantation (2002), Vol. 73, No. 4, pp. 657-60).
• the compounds of the invention may be tested for their use in treating rheumatoid arthritis by using the CAIA mouse model (see Phadke, K. et al., "Evaluation of the effects of various anti-arthritic drugs on type Il collagen-induced mouse arthritis model", lmmunopharmacology (1985),Vol. 10, No. 1 , pp. 51-60).
• the compounds of the invention may be tested for their use in treating osteoarthritis by using the STR/ORT mouse model (see Brewster, M. et ai, "Ro 32-3555, an orally active collagenase selective inhibitor, prevents structural damage in the STR/ORT mouse model of osteoarthritis", Arthritis. Rheum. (1998), Vol. 41 , No. 9, pp. 1639-44).
• the compounds of the invention may be tested for their use in treating osteoporosis by using the ovariectomized rat model (see Wronski, T.J. et al., "Endocrine and pharmacological suppressors of bone turnover protect against osteopenia in ovariectomized rats", Endocrinology (1989), Vol. 125, no. 2, pp 810-6) or the ovariectomized mouse model (see Alexander, J. M. et al., "Human parathyroid hormone 1-34 reverses bone loss in ovariectomized mice", J Bone Miner Res. (2001 ), Vol. 16, no. 9, pp 1665-73; Fujioka, M. et al., "Equol, a metabolite of daidzein, inhibits bone loss in ovariectomized mice", J Nut. (2004), Vol. 134, no. 10, pp 2623-7).
• the compounds of the invention may be tested for their use in treating cataracts by using the H 2 O 2 -induced model (see Kadoya, K. et al., "Role of calpain in hydrogen peroxide induced cataract", Curr. Eye Res. (1993), Vol. 12, No. 4, pp. 341-6) or the Emory mouse model (see Sheets, N. L. et al., "Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in Emory mouse cataract", Invest. Ophthalmol. Vis. Sci. (2002), Vol. 43, No. 6, pp. 1870-5).
• compositions of the invention can be prepared by combining a compound of the invention with an appropriate pharmaceutically acceptable carrier, diluent or excipient, and may be formulated into preparations in solid, semi-solid, liquid or gaseous forms, such as tablets, capsules, powders, granules, ointments, solutions, suppositories, injections, inhalants, gels, microspheres, and aerosols.
• compositions of the invention are formulated so as to allow the active ingredients contained therein to be bioavailable upon administration of the composition to a patient.
• Compositions that will be administered to a subject or patient take the form of one or more dosage units, where for example, a tablet may be a single dosage unit, and a container of a compound of the invention in aerosol form may hold a plurality of dosage units.
• compositions to be administered will, in any event, contain a therapeutically effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, for treatment of a disease or condition of interest in accordance with the teachings of this invention.
• a pharmaceutical composition of the invention may be in the form of a solid or liquid.
• the carrier(s) are particulate, so that the compositions are, for example, in tablet or powder form.
• the carrier(s) may be liquid, with the compositions being, for example, an oral oil, injectable liquid or an aerosol, which is useful in, for example, inhalatory administration.
• the pharmaceutical composition is preferably in either solid or liquid form, where semi-solid, semi-liquid, suspension and gel forms are included within the forms considered herein as either solid or liquid.
• the pharmaceutical composition may be formulated into a powder, granule, compressed tablet, pill, capsule, chewing gum, wafer or the like form.
• a solid composition will typically contain one or more inert diluents or edible carriers.
• binders such as carboxymethylcellulose, ethyl cellulose, microcrystalline cellulose, gum tragacanth or gelatin; excipients such as starch, lactose or dextrins, disintegrating agents such as alginic acid, sodium alginate, Primogel, corn starch and the like; lubricants such as magnesium stearate or Sterotex; glidants such as colloidal silicon dioxide; sweetening agents such as sucrose or saccharin; a flavoring agent such as peppermint, methyl salicylate or orange flavoring; and a coloring agent.
• excipients such as starch, lactose or dextrins, disintegrating agents such as alginic acid, sodium alginate, Primogel, corn starch and the like
• lubricants such as magnesium stearate or Sterotex
• glidants such as colloidal silicon dioxide
• sweetening agents such as sucrose or saccharin
• a flavoring agent such as peppermint, methyl sal
• the pharmaceutical composition when in the form of a capsule, for example, a gelatin capsule, it may contain, in addition to materials of the above type, a liquid carrier such as polyethylene glycol or oil.
• a liquid carrier such as polyethylene glycol or oil.
• the pharmaceutical composition may be in the form of a liquid, for example, an elixir, syrup, solution, emulsion or suspension.
• the liquid may be for oral administration or for delivery by injection, as two examples.
• preferred composition contain, in addition to the present compounds, one or more of a sweetening agent, preservatives, dye/colorant and flavor enhancer.
• a surfactant, preservative, wetting agent, dispersing agent, suspending agent, buffer, stabilizer and isotonic agent may be included.
• the liquid pharmaceutical compositions of the invention may include one or more of the following adjuvants: sterile diluents such as water for injection, saline solution, preferably physiological saline, Ringer's solution, isotonic sodium chloride, fixed oils such as synthetic mono or diglycerides which may serve as the solvent or suspending medium, polyethylene glycols, glycerin, propylene glycol or other solvents; antibacterial agents such as benzyl alcohol or methyl paraben; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose.
• the parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
• Physiological saline is a preferred adjuvant.
• a liquid pharmaceutical composition of the invention intended for either parenteral or oral administration should contain an amount of a compound of the invention such that a suitable dosage will be obtained. Typically, this amount is at least 0.01 % of a compound of the invention in the composition. When intended for oral administration, this amount may be varied to be between 0.1 and about 70% of the weight of the composition.
• Preferred oral pharmaceutical compositions contain between about 4% and about 75% of the compound of the invention.
• Preferred pharmaceutical compositions and preparations according to the present invention are prepared so that a parenteral dosage unit contains between 0.01 to 10% by weight of the compound prior to dilution of the invention.
• the pharmaceutical composition of the invention may be intended for topical administration, in which case the carrier may suitably comprise a solution, emulsion, ointment or gel base.
• the base for example, may comprise one or more of the following: petrolatum, lanolin, polyethylene glycols, bee wax, mineral oil, diluents such as water and alcohol, and emulsifiers and stabilizers.
• Thickening agents may be present in a pharmaceutical composition for topical administration.
• the composition may include a transdermal patch or iontophoresis device.
• Topical formulations may contain a concentration of the compound of the invention from about 0.1 to about 10% w/v (weight per unit volume).
• the pharmaceutical composition of the invention may be intended for rectal administration, in the form, for example, of a suppository, which will melt in the rectum and release the drug.
• the composition for rectal administration may contain an oleaginous base as a suitable nonirritating excipient.
• bases include, without limitation, lanolin, cocoa butter and polyethylene glycol.
• the pharmaceutical composition of the invention may include various materials, which modify the physical form of a solid or liquid dosage unit.
• the composition may include materials that form a coating shell around the active ingredients.
• the materials that form the coating shell are typically inert, and may be selected from, for example, sugar, shellac, and other enteric coating agents.
• the active ingredients may be encased in a gelatin capsule.
• the pharmaceutical composition of the invention in solid or liquid form may include an agent that binds to the compound of the invention and thereby assists in the delivery of the compound. Suitable agents that may act in this capacity include a monoclonal or polyclonal antibody, a protein or a liposome.
• the pharmaceutical composition of the invention may consist of dosage units that can be administered as an aerosol.
• aerosol is used to denote a variety of systems ranging from those of colloidal nature to systems consisting of pressurized packages. Delivery may be by a liquefied or compressed gas or by a suitable pump system that dispenses the active ingredients. Aerosols of compounds of the invention may be delivered in single phase, bi-phasic, or tri-phasic systems in order to deliver the active ingredient(s).
• the pharmaceutical compositions of the invention may be prepared by methodology well known in the pharmaceutical art.
• a pharmaceutical composition intended to be administered by injection can be prepared by combining a compound of the invention with sterile, distilled water so as to form a solution.
• a surfactant may be added to facilitate the formation of a homogeneous solution or suspension.
• Surfactants are compounds that non-covalently interact with the compound of the invention so as to facilitate dissolution or homogeneous suspension of the compound in the aqueous delivery system.
• the compounds of the invention are administered in a therapeutically effective amount, which will vary depending upon a variety of factors including the activity of the specific compound employed; the metabolic stability and length of action of the compound; the age, body weight, general health, sex, and diet of the patient; the mode and time of administration; the rate of excretion; the drug combination; the severity of the particular disorder or condition; and the subject undergoing therapy.
• a therapeutically effective daily dose is (for a 70 kg mammal) from about 0.001 mg/kg (i.e., 0.07 mg) to about 100 mg/kg (i.e., 7.0 gm); preferaby a therapeutically effective dose is (for a 70 kg mammal) from about 0.01 mg/kg (i.e., 0.7 mg) to about 50 mg/kg (i.e., 3.5 gm); more preferably a therapeutically effective dose is (for a 70 kg mammal) from about 1 mg/kg (i.e., 70 mg) to about 25 mg/kg (i.e., 1.75 gm).
• Compounds of the invention, or pharmaceutically acceptable salts thereof, may also be administered simultaneously with, prior to, or after administration of one or more other therapeutic agents.
• Such combination therapy includes administration of a single pharmaceutical dosage formulation which contains a compound of the invention and one or more additional active agents, as well as administration of the compound of the invention and each active agent in its own separate pharmaceutical dosage formulation.
• a compound of the invention and the other active agent can be administered to the patient together in a single oral dosage composition such as a tablet or capsule, or each agent administered in separate oral dosage formulations.
• the compounds of the invention and one or more additional active agents can be administered at essentially the same time, i.e., concurrently, or at separately staggered times, i.e., sequentially; combination therapy is understood to include all these regimens.
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 , -N(R 2 )-R 4 -C(O)OR 3 , or R 1 is an ⁇ /-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2
• each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroaryl;
• each R 4 is independently selected from the group consisting of a direct bond or an optionally substituted straight or branched alkylene chain; and as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof.
• Suitable protecting groups for amino, amidino and guanidino include benzyl, f-butoxycarbonyl, benzyloxycarbonyl, and the like.
• Suitable protecting groups for mercapto include -C(O)-R" (where R" is alkyl, aryl or arylalkyl), p-methoxybenzyl, trityl and the like.
• Suitable protecting groups for carboxylic acids include alkyl, aryl or arylalkyl esters. Protecting groups may be added or removed in accordance with standard techniques, which are known to one of ordinary skill in the art and as described herein.
• protecting groups are described in detail in Green, T.W. and P. G. M. Wuts, Protective Groups in Organic Synthesis (1999), 3rd Ed., Wiley.
• the protecting group may also be a polymer resin such as a Wang resin, Rink resin or a 2-chlorotrityl-chloride resin.
• protected derivatives of compounds of this invention may not possess pharmacological activity as such, they may be administered to a mammal and thereafter metabolized in the body to form compounds of the invention which are pharmacologically active. Such derivatives may therefore be described as "prodrugs".
• starting components may be obtained from sources such as Sigma Aldrich, Lancaster Synthesis, Inc., Maybridge, Matrix Scientific, TCI, and Fluorochem USA, etc. or synthesized according to sources known to those skilled in the art (see, for example, Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, 5th edition (Wiley, December 2000)) or prepared as described in this invention.
• 1 H NMR spectra were recorded in CDCI 3 , DMSOd 6 , CD 3 OD, Acetone-d 6 with trimethylsilane (TMS) as internal reference using Gemini 300 MHz instrument. Reagents and solvents were purchased from commercial sources and used without further purification.
• Flash column chromatography was conducted using silica gel (230-400 mesh) under a positive pressure of nitrogen.
• LCMS spectra for purity and mass were recorded using Waters LCMS instruments. Deionized water was used to dilute the reactions and wash the products. Brine used was prepared by dissolving sodium chloride into deionized water to saturation point.
• compounds of formula (Ia) are prepared, as set forth by Reaction Scheme 1 , by first treating a compound of formula (A) (where the phenyl groups therein may be replaced with other suitable groups or suitably substituted groups known to one skilled in the art) (1.1 equiv) with an equivalent amount of an aniline of formula (B) in an polar solvent, including, but not limited to, isopropyl alcohol, at ambient temperatures overnight.
• a polar solvent including, but not limited to, isopropyl alcohol
• the diarylisourea product of formula (C) generally precipitates and isolation can be accomplished via filtration, washing with an appropriate solvent, and drying. Hydrazine hydrate of formula (D) (2 equivalents) is added to a slurry of the compound of formula (C) in an alcohol or other appropriate solvent.
• the ring formation reaction occurs at ambient temperature and the product triazole of formula (Ia) can be isolated by standard isolation techniques.
• Compounds of formula (Ia) can be subsequently treated with an appropriately substituted alkylating or acylating agent under standard conditions to form compounds of formula (I), as set forth in the Summary of the Invention, wherein R 2 and R 3 in the compounds so prepared are as described above in the Summary of the Invention for compounds of formula (I).
• R 1 is -N(R 2 )R 3 , -N(R 2 )C(O)R 3 , -N(R 2 )-R 4 -C(O)OR 3 , or R 1 is an A/-heterocyclyl optionally substituted by one or more substituents selected from the group consisting of halo and -R 4 -C(O)OR 2
• each R 2 and R 3 is independently selected from the group consisting of hydrogen, alkyl, alkenyl, optionally substituted aralkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl and optionally substituted heteroaryl;
• each R 4 is independently selected from the group consisting of a direct bond or an optionally substituted straight or branched alkylene chain; and as an isolated stereoisomer or mixture thereof, or a pharmaceutically acceptable salt thereof; can be prepared using the synthetic route outlined in Reaction Scheme 1 in
• compounds of formula (Ib) are isolated as minor isomers along with compounds of formula (Ia) as major isomers, e.g., during column chromatography as described herein.
• Compounds of formula (C-1 ) are compounds of formula (C), as set forth above in
• Reaction Scheme 1 where R 1 is pyrrolidin-1-yl. They can be prepared according to the method described below in relation to Reaction Scheme 2: REACTION SCHEME 2
• Compounds of formula (Ca) and formula (A) are commercially available or can be prepared according to methods described herein or known to one skilled in the art.
• Compounds of formula (Ba) are compounds of formula (B), as set forth above in Reaction Scheme 1.
• compounds of formula (C-1 ) are prepared, for example, as set forth above in Reaction Scheme 2, by nitration of the benzo[7]annulene of formula (Ca) to form the nitro compound of formula (Cb). Reductive amination of the keto group in the ketone of formula (Cb) yields the pyrrolidine-substituted compound of formula (Cc).
• transaminases are enzymes that catalyze a transamination reaction between an amino-donor molecule (such as an amine or amino acid) and an amino-acceptor molecule (such as a ketone or an ⁇ -keto acid).
• enzymatic transamination involves removing the amino group from the amino-donor molecule (leaving behind a carbonyl group) and transferring the amino group to the amino-acceptor molecule (or ⁇ -keto acid) by converting the ketone moiety therein to an amine (or an amino acid).
• a description of transaminases and their use in stereoselective synthesis can be found in "Transminations. Enzyme Catalysis in Organic Synthesis (2 nd Edition) (2002)", by J. David Rozzell and Andreas S. Bommarius, pp. 873- 893, which is incorporated in full by reference herein.
• Transaminases are particularly suitable for the enzymatic synthesis of chiral amines from the corresponding ketone precursors. Commercially available transaminases can be used to achieve a chiral enzymatic amination of a desired starting material in the preparation of the compounds of the invention.
• a ketone of the following formula (i) where n and m are the same and are 0, 1 or 2 and R 7 is nitro, halo or -C(O)OR 2 (where R 2 is as described above in the Summary of the Invention) can be converted under suitable conditions to the corresponding (S)-enantiomer and (R)-enantiomer wherein the carbon to which the amino group is attached is either in the (S) or the (R) configuration, respectively, by utilizing a (S)-specific tranasminase and an amino donor molecule, such as L-alanine, or a (ft)-specific transaminase and an amino donor molecule, such as L-alanine, as shown below:
• the appropriate transaminase to convert the cyclic ketone of formula (i) into the appropriate enantiomer
• the appropriate enantiomer can be isolated in greater than 80% ee and preferably greater than 90% ee.
• Reaction 3 where the compound of formula (Cb-1 ) is a compound of formula (i) as described above and PG represents a nitrogen protecting group, illustrates a method of preparing a chiral compound of the invention utilizing a transaminase as described above:
• Compounds of formula (Cb-1 ) are commercially available, or can be prepared by methods known to one skilled in the art.
• Compounds of formula (D-1 ) can be prepared according to methods known to one skilled in the art or by methods disclosed herein.
• the (S)-specific transaminase is commercially available from Codexis.
• the (S)-specific transaminase is ATA-103 from Codexis.
• compounds of formula (la-1 ) are prepared by the method disclosed above in Reaction Scheme 3 by first converting the ketone of formula (Cb-1 ) into the chiral compound of formula (Cb-2) wherein the amino group from an amino donor molecule, preferably L-alanine, is transferred to the ketone of formula (Cb-1 ) through an enzymatic transamination reaction under suitable conditions.
• the ketone of formula (Cb-1 ) is treated with a excess molar amount of an amino donor molecule in the the presence of a catalytic amount of a transaminase, preferably a (S)-specific transaminase, and a stoichiometric or excess stoichiometric amount of a pyruvate reductase mixture that reduces (deactivates) the 2-keto acid side product, thereby driving the reaction into the desired direction.
• a catalytic amount of a transaminase preferably a (S)-specific transaminase
• a stoichiometric or excess stoichiometric amount of a pyruvate reductase mixture that reduces (deactivates) the 2-keto acid side product, thereby driving the reaction into the desired direction.
• the pyruvate reductase mixture is PRM-102 from Codexix.
• the reaction is conducted at ambient temperature, at a pH of between about 7.5 and about 8.0, and for a period of time of between about 24 hours and about 6 days, preferably for about 4 days.
• the chiral compound of formula (Cb-2) is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the transamination reaction can be driven to completion by coupling the reaction to a second reaction that consumes the 2-keto acid by-product in an essentially irreversible step, as described in more detail in "Transminations. Enzyme Catalysis in Organic Synthesis (2 nd Edition) (2002)", by J. David Rozzell and Andreas S. Bommarius, pp. 873-893.
• the amino group of the chiral compound of formula (Cb-2) is then protected by standard nitrogen protecting procedures to yield the compound of formula (Cb-3), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the compound of formula (Cb-3) is then treated to standard reducing conditions, such as treatment with H 2 /Pd, to produce the corresponding aniline compound of formula (Cb-4), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the compound of formula (Cb-4) is then treated with diphenyl cyanocarbonimidate of formula (A) to produce the compound of formula (Cb-5), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the compound of (Cb-5) is then treated with a compound of formula (D-1 ) in the presence of an aprotic solvent, preferably toluene, at a temperature of between about 80 °C and about 100 0 C for a period of time of between about 12 hours and about 36 hours, preferably for about 24 hours, to yield a compound of formula (la-1 ), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• Compound of formula (la-1) is a compound of formula (Ia), as set forth above.
• the protecting group on the compound of formula (la-1 ) can be removed under standard deprotecting conditions known to one skilled in the art, such as acid hydrolysis, to produce a compound of formula (la-2), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the compound of formula (la-2) can be further treated with the appropriate aldehyde or ketone under standard reductive amination conditions to yield a compound of formula (la-3), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• compounds of formula (D-1 ) are prepared, as set forth above in Reaction Scheme 3, by first dissolving the compound of formula (Da) (1.0 equiv) in an anhydrous aprotic polar solvent or mixture of such solvents, for example, tetrahydrofuran with hexamethylphosphoramide (HMPA) (1.2 equiv). The resulting solution is stirred at ambient temperature for about 10 minutes and then cooled to a temperature of between about -10 0 C and about 5 0 C, preferably at 0 0 C.
• HMPA hexamethylphosphoramide
• Ethyl bromoacetate (2.5 equiv) is then added to the resulting anion of (Da) and the resulting mixture is stirred for additional period of time of between about 5 minutes and 15 minutes, preferably for about 10 minutes, and then allowed to warm to ambient temperature and stirred at ambient temperature for a period of time of between about 30 minutes and 3 hours, preferably for about 2 hours.
• the compound of formula (Db) is then isolated from the reaction mixture by standard isolation techniques known to one skilled in the art, such as solvent evaporation and purification by flash column chromatography.
• the compound of formula (Db) is then treated under basic hydrolysis conditions to form the compound of formula (Dc), which is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• the compound of formula (Dc) (1.0 equiv) is then treated with hydrazine hydrate
• a mixture of the compound of formula (De) and phosphoryl chloride is refluxed for a period of time of between about 1 hour and 3 hours, preferably for about 2 hours to aromatize and chlorinate the ring containing the N-N linkage.
• the compound of formula (Df) is isolated from the reaction mixture by standard isolation techniques known to one skilled in the art.
• a protic solvent such as ethanol
• All compounds of the invention which exist in free base or acid form can be converted to their pharmaceutically acceptable salts by treatment with the appropriate inorganic or organic base or acid by methods known to one of ordinary skill in the art. Salts of the compounds of the invention can be converted to their free base or acid form by standard techniques known to one skilled in the art.
• a compound of formula (Cc) Pyrrolidine (0.85 g, 12 mmol) and 2-nitro-7-oxo-6,7,8,9-tetrahydro-5/-/- benzo[7]annulene (2.05 g, 10 mmol) were mixed in 1 ,2-dchloroethane (35 mL) and then treated with NaBH(OAc) 3 (3.18 g, 15 mmo! and AcOH (0.60 g, 10 mmol). The mixture was stirred at ambient temperature under a N 2 atmosphere overnight. The reaction mixture was quenched with saturated NaHCO 3 , and the product was extracted with EtOAc (3 x 30 mL). The organic layers were combined and dried over NaSO 4 .
• the crude solid was then heated at 100 0 C for 1 h with a mixture of acetic acid (60 mL), water (30 mL) and zinc dust (6 g).
• the reaction mixture was cooled to ambient temperature and filtered.
• the filtrate was extracted with ethyl acetate.
• the organic layer was washed three times with saturated sodium chloride solution, then dried over anhydrous sodium sulfate and concentrated under vacuum.
• the crude residue was heated with ethanol (25 mL) and hydrazine monohydrate (10 mL) under reflux for 3 h.
• the compounds of the invention were tested in the following assay for their ability to inhibit AxI activity.
• REAGENTS AND BUFFERS Cell culture plate: 96 well assay plate (Coming 3610), white, clear bottom, tissue- culture treated. Cells: HeIa cells. Starvation medium: For AxI stimulation: 0.5% FCS (fetal calf serum) in DMEM, plus Axl/Fc (extracellular domain of AXL fused to imunoglobulin Fc region) (R&D,
• Fixing buffer 4% formaldehyde in PBS.
• Wash buffer 0.1% TritonX-100 in PBS.
• Quenching buffer 3% H 2 O 2 , 0.1% Azide in wash buffer, Azide and hydrogen peroxide
• Blocking buffer 5% BSA in TBST (tris buffered saline plus 0.1 % Tween 20).
• Chemiluminescent working solution (Pierce, 37030): SuperSignal ELISA (enzyme linked immunosorbant assay) Pico Chemiluminescent substrate.
• Crystal Violet solution Stock : 2.5% Crystal violet in methanol, filtered and kept at ambient temperature. The working solution: dilute the stock 1 :20 with PBS immediately before use. 10% SDS: working solution: 5% SDS (sodium dodecylsulfate), diluted in PBS
• Dav 1 A 96 well TC (tissue culture treated) plate was coated with 10 ⁇ g/mL poly-L-
• the cells were pre-treated with test compounds by adding 100 ⁇ L of 2X test compound to the starvation medium on the cells. The cells were incubated at 37 0 C for 1 hr before stimulation. The cells were stimulated by Axl-antibody cross-linking as follows: A 5X i st /2 nd
• AxI antibody mixture was made (37.5 ⁇ g/ml_ 1 st / 100 ⁇ g/ml_ 2 nd ) in starvation medium and nutated at 4°C with thorough mixing for 1-2 hours for clustering. The resulting mix was warmed to 37°C. 50 ⁇ L of 5X AxI 1 st /2 nd of antibody cluster was added to the cells and the ceils were incubated at 37°C for 5 min. After 5 minutes stimulation, the plate was flicked to remove medium and the plate was tapped onto paper towels. Formaldehyde (4.0% in PBS, 100 ⁇ L) was added to fix the cells and the cells were incubated at ambient temperature for 20 min without shaking.
• the cells were washed with a plate washer buffer to remove the formaldehyde solution.
• the plate was flicked to removed excess wash buffer and tapped onto paper towels.
• Quenching buffer 100 ⁇ L was added to each well and the cells were incubated at ambient temperature for 20 minutes without shaking.
• the cells were washed with a plate washer buffer to remove the quenching buffer.
• Blocking buffer 100 ⁇ L was added and the cells were incubated at ambient temperature for at least an hour with gentle shaking.
• the cells were washed with a plate washer buffer and diluted primary antibody (50 ⁇ L) was added to each well (blocking buffer was added to the negative control wells instead). The plates were incubated overnight at 4° C with gentle shaking. Day 3: The wash buffer was removed, diluted secondary antibody (100 ⁇ L) was added, and the cells were incubated at ambient temperature for 1 hour with gentle shaking. During the incubation, the chemiluminescent reagent was brought to ambient temperature.
• the secondary antibody was removed by washing the cells 1X with wash buffer, 1 X with PBS by plate washer.
• the PBS was removed from the plate and the chemiluminescent reagent (80 ⁇ L: 40 ⁇ L A and 40 ⁇ L B) was added to each well at ambient temperature.
• the resulting chemiluminescence was read with a Luminomitor within 10 minutes to minimize changes in signal intensity.
• the cells were washed 1X with wash buffer and 1X with PBS by plate washer. The plate was tapped onto paper towels to remove excess liquid from wells and air-dried at ambient temperature for 5 minutes.
• Crystal Violet working solution (60 ⁇ l_) was added to each well and the cells were incubated at ambient temperature for 30 min. The crystal violet solution was removed, and the wells were rinsed with PBS, then washed 3X with PBS (200 ⁇ l_) for 5 minutes each.

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