WO2009088189A3 - 형질전환된 복제개의 생산방법 - Google Patents

형질전환된 복제개의 생산방법 Download PDF

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Publication number
WO2009088189A3
WO2009088189A3 PCT/KR2009/000028 KR2009000028W WO2009088189A3 WO 2009088189 A3 WO2009088189 A3 WO 2009088189A3 KR 2009000028 W KR2009000028 W KR 2009000028W WO 2009088189 A3 WO2009088189 A3 WO 2009088189A3
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WIPO (PCT)
Prior art keywords
ovum
transplanted
nucleus
canidae
denucleated
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PCT/KR2009/000028
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English (en)
French (fr)
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WO2009088189A2 (ko
Inventor
이병천
김민규
장구
오현주
홍소군
박정은
강정택
라정찬
김태완
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재단법인 서울대학교산학협력재단
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Priority to JP2010541404A priority Critical patent/JP2011508600A/ja
Priority to EP09700180A priority patent/EP2232985A4/en
Publication of WO2009088189A2 publication Critical patent/WO2009088189A2/ko
Publication of WO2009088189A3 publication Critical patent/WO2009088189A3/ko

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 형질전환 복제개의 생산방법에 관한 것으로, 보다 구체적으로는 개의 난자로부터 핵을 제거하여 탈핵 난자를 제조하고 상기 탈핵 난자에 목적 유전자로 형질전환시킨 세포를 이식하여 핵 이식란을 제조한 다음, 이를 대리모에 이식하는 것을 특징으로 하는, 목적 유전자가 도입된 복제개의 생산방법에 관한 것이다. 본 발명은 외래성 유전자의 성공적 도입에 의해 질환모델 동물의 대량 생산가능성을 확인하였으며, 이는 우량 품종의 번식, 이종이식, 질환 모델동물 등 수의학, 인류학 및 의학연구 분야에 유용하다.
PCT/KR2009/000028 2008-01-04 2009-01-05 형질전환된 복제개의 생산방법 WO2009088189A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2010541404A JP2011508600A (ja) 2008-01-04 2009-01-05 形質転換された複製犬の生産方法
EP09700180A EP2232985A4 (en) 2008-01-04 2009-01-05 PROCESS FOR PREPARING CLONED TRANSGENIC CANIDAE

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0001106 2008-01-04
KR20080001106 2008-01-04

Publications (2)

Publication Number Publication Date
WO2009088189A2 WO2009088189A2 (ko) 2009-07-16
WO2009088189A3 true WO2009088189A3 (ko) 2009-10-29

Family

ID=40853577

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/000028 WO2009088189A2 (ko) 2008-01-04 2009-01-05 형질전환된 복제개의 생산방법

Country Status (4)

Country Link
EP (1) EP2232985A4 (ko)
JP (1) JP2011508600A (ko)
KR (1) KR101105248B1 (ko)
WO (1) WO2009088189A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101939570B1 (ko) * 2018-05-08 2019-01-17 주식회사 엠케이바이오텍 복제 개의 생산방법 및 그에 따른 복제 개
CN108977408B (zh) * 2018-08-14 2022-06-14 北京希诺谷生物科技有限公司 利用体外成熟犬卵母细胞制备体外受精犬的方法
WO2020091471A1 (ko) * 2018-10-31 2020-05-07 서울대학교산학협력단 근육 특이적 pck1 과발현 형질전환 개 생산

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040064845A1 (en) * 2000-12-05 2004-04-01 Smith Lawrence Charles Method of cloning animals
US6906238B2 (en) * 2000-03-15 2005-06-14 University Of Georgia Research Foundation, Inc. Effective nuclear reprogramming in mammals using CDK2 inhibitors
KR20070013432A (ko) * 2005-07-26 2007-01-31 재단법인서울대학교산학협력재단 복제된 개과 동물 및 이의 생산 방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
KR100342437B1 (ko) 1999-06-30 2002-07-04 황우석 체세포 복제동물의 생산을 위한 난자의 탈핵방법
US20030217378A1 (en) * 2002-04-05 2003-11-20 The University Of Georgia Research Foundation, Inc Cloning using rapidly matured oocytes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6906238B2 (en) * 2000-03-15 2005-06-14 University Of Georgia Research Foundation, Inc. Effective nuclear reprogramming in mammals using CDK2 inhibitors
US20040064845A1 (en) * 2000-12-05 2004-04-01 Smith Lawrence Charles Method of cloning animals
KR20070013432A (ko) * 2005-07-26 2007-01-31 재단법인서울대학교산학협력재단 복제된 개과 동물 및 이의 생산 방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GIBBOONS, J. ET AL.: "Enhanced survivability of cloned calves derived from roscovitine- treated adult somatic cells.", BIOLOGY OF REPRODUCTION., vol. 66, 2002, pages 895 - 900, XP008003993 *
HINRICHS, K. ET AL.: "Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin.", REPRODUCTION, vol. 134, 2007, pages 319 - 325, XP008129245 *
See also references of EP2232985A4 *

Also Published As

Publication number Publication date
KR101105248B1 (ko) 2012-01-17
EP2232985A2 (en) 2010-09-29
JP2011508600A (ja) 2011-03-17
WO2009088189A2 (ko) 2009-07-16
EP2232985A4 (en) 2011-02-16
KR20090075637A (ko) 2009-07-08

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