WO2009051109A1 - 異種タンパク質を高生産する細胞の作製方法 - Google Patents

異種タンパク質を高生産する細胞の作製方法 Download PDF

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Publication number
WO2009051109A1
WO2009051109A1 PCT/JP2008/068589 JP2008068589W WO2009051109A1 WO 2009051109 A1 WO2009051109 A1 WO 2009051109A1 JP 2008068589 W JP2008068589 W JP 2008068589W WO 2009051109 A1 WO2009051109 A1 WO 2009051109A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
high productivity
constructing
foreign protein
productivity
Prior art date
Application number
PCT/JP2008/068589
Other languages
English (en)
French (fr)
Inventor
Hisahiro Tabuchi
Tomoya Sugiyama
Original Assignee
Chugai Seiyaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Priority to US12/733,815 priority Critical patent/US9802993B2/en
Priority to BRPI0818039-3A2A priority patent/BRPI0818039A2/pt
Priority to JP2009538094A priority patent/JP5337043B2/ja
Priority to EP08838749.3A priority patent/EP2194126B1/en
Priority to RU2010119558/10A priority patent/RU2486245C2/ru
Publication of WO2009051109A1 publication Critical patent/WO2009051109A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/303—Liver or Pancreas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Abstract

　ポリペプチドを高生産する細胞及びその作製方法を提供する。 　タウリントランスポーターを強発現し、且つ所望のポリペプチドをコードするDNAが導入された細胞を高濃度メトトレキセートの存在下で培養し、生存する細胞から所望のポリペプチドを高産生する細胞を選択することを含む、所望のポリペプチドを高産生する細胞の作製方法。  
PCT/JP2008/068589 2007-10-15 2008-10-14 異種タンパク質を高生産する細胞の作製方法 WO2009051109A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/733,815 US9802993B2 (en) 2007-10-15 2008-10-14 Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate
BRPI0818039-3A2A BRPI0818039A2 (pt) 2007-10-15 2008-10-14 Método para produzir uma célula capaz de produção de alto rendimento de heteroproteínas.
JP2009538094A JP5337043B2 (ja) 2007-10-15 2008-10-14 異種タンパク質を高生産する細胞の作製方法
EP08838749.3A EP2194126B1 (en) 2007-10-15 2008-10-14 Method of constructing cells with high productivity of foreign protein
RU2010119558/10A RU2486245C2 (ru) 2007-10-15 2008-10-14 Способ получения клетки, способной продуцировать гетеропротеины с высоким выходом

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-267384 2007-10-15
JP2007267384 2007-10-15

Publications (1)

Publication Number Publication Date
WO2009051109A1 true WO2009051109A1 (ja) 2009-04-23

Family

ID=40567373

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068589 WO2009051109A1 (ja) 2007-10-15 2008-10-14 異種タンパク質を高生産する細胞の作製方法

Country Status (6)

Country Link
US (1) US9802993B2 (ja)
EP (1) EP2194126B1 (ja)
JP (1) JP5337043B2 (ja)
BR (1) BRPI0818039A2 (ja)
RU (1) RU2486245C2 (ja)
WO (1) WO2009051109A1 (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010123014A1 (ja) * 2009-04-22 2010-10-28 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
WO2012137683A1 (ja) * 2011-04-01 2012-10-11 中外製薬株式会社 組換えポリペプチドの製造方法
US8697397B2 (en) 2007-08-07 2014-04-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
WO2014058025A1 (ja) 2012-10-10 2014-04-17 中外製薬株式会社 改変宿主細胞の樹立方法
US8796007B2 (en) 2007-03-15 2014-08-05 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9068212B2 (en) 2007-10-24 2015-06-30 Chugai Seiyaku Kabushiki Kaisha Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter
US9238687B2 (en) 2006-04-13 2016-01-19 Chugai Seiyaku Kabushiki Kaisha Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide
US9802993B2 (en) 2007-10-15 2017-10-31 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate

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KR102467730B1 (ko) * 2014-08-11 2022-11-15 에프. 호프만-라 로슈 아게 진핵생물 세포의 비생산률 증가를 위한 방법
GB2596803A (en) * 2020-07-06 2022-01-12 Petmedix Ltd Expression vector production & high-throughput cell screening

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9238687B2 (en) 2006-04-13 2016-01-19 Chugai Seiyaku Kabushiki Kaisha Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide
US8796007B2 (en) 2007-03-15 2014-08-05 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9181540B2 (en) 2007-03-15 2015-11-10 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9499618B2 (en) 2007-08-07 2016-11-22 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US8697397B2 (en) 2007-08-07 2014-04-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US9284374B2 (en) 2007-08-07 2016-03-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US9802993B2 (en) 2007-10-15 2017-10-31 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate
US9068212B2 (en) 2007-10-24 2015-06-30 Chugai Seiyaku Kabushiki Kaisha Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter
US8741601B2 (en) 2009-04-22 2014-06-03 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell capable of high-yield production of heteroproteins
WO2010123014A1 (ja) * 2009-04-22 2010-10-28 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
US11905325B2 (en) 2011-04-01 2024-02-20 Chugai Seiyaku Kabushiki Kaisha Recombinant polypeptide production method
US11028149B2 (en) 2011-04-01 2021-06-08 Chugai Seiyaku Kabushiki Kaisha Recombinant polypeptide production method
JPWO2012137683A1 (ja) * 2011-04-01 2014-07-28 中外製薬株式会社 組換えポリペプチドの製造方法
CN103459608A (zh) * 2011-04-01 2013-12-18 中外制药株式会社 重组多肽的制造方法
WO2012137683A1 (ja) * 2011-04-01 2012-10-11 中外製薬株式会社 組換えポリペプチドの製造方法
WO2014058025A1 (ja) 2012-10-10 2014-04-17 中外製薬株式会社 改変宿主細胞の樹立方法
KR20210014223A (ko) 2012-10-10 2021-02-08 추가이 세이야쿠 가부시키가이샤 개변 숙주 세포의 수립 방법
KR20150065812A (ko) 2012-10-10 2015-06-15 추가이 세이야쿠 가부시키가이샤 개변 숙주 세포의 수립 방법

Also Published As

Publication number Publication date
US20110003334A1 (en) 2011-01-06
BRPI0818039A2 (pt) 2014-10-14
JP5337043B2 (ja) 2013-11-06
EP2194126A4 (en) 2011-01-19
US9802993B2 (en) 2017-10-31
RU2010119558A (ru) 2011-11-27
JPWO2009051109A1 (ja) 2011-03-03
EP2194126A1 (en) 2010-06-09
EP2194126B1 (en) 2014-01-15
RU2486245C2 (ru) 2013-06-27

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