WO2009034790A1 - 蛍光発生分子 - Google Patents

蛍光発生分子 Download PDF

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Publication number
WO2009034790A1
WO2009034790A1 PCT/JP2008/063871 JP2008063871W WO2009034790A1 WO 2009034790 A1 WO2009034790 A1 WO 2009034790A1 JP 2008063871 W JP2008063871 W JP 2008063871W WO 2009034790 A1 WO2009034790 A1 WO 2009034790A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent
molecule
skeleton
signals
fluorescent molecule
Prior art date
Application number
PCT/JP2008/063871
Other languages
English (en)
French (fr)
Inventor
Hiroshi Abe
Yoshihiro Ito
Kazuhiro Furukawa
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Priority to JP2009532110A priority Critical patent/JP5419278B2/ja
Priority to US12/677,014 priority patent/US8084201B2/en
Priority to CN200880115960.5A priority patent/CN101896491B/zh
Priority to EP08792084A priority patent/EP2204371B1/en
Publication of WO2009034790A1 publication Critical patent/WO2009034790A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

　本発明の目的は、安定性が高く（即ち、長期間において活性である）、かつ高感度であり、微量の遺伝子シグナルを増幅し、観察することを可能とする、遺伝子解析用の蛍光on/off型蛍光化合物（蛍光発生分子システム）を提供することである。本発明によれば、フルオレセイン骨格などの蛍光物質骨格を有し、かつ分子内に－Ｏ－Ｃ（Ｙ１）（Ｙ２）―Ｎ３で示される基（式中、Ｙ１及びＹ２は、水素原子、炭素数１から６のアルキル基、炭素数１から６のアルコキシ基、炭素数６から１０のアリール基、又はシアノ基を示す）を有する無蛍光性分子が提供される。  
PCT/JP2008/063871 2007-09-14 2008-08-01 蛍光発生分子 WO2009034790A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2009532110A JP5419278B2 (ja) 2007-09-14 2008-08-01 蛍光発生分子
US12/677,014 US8084201B2 (en) 2007-09-14 2008-08-01 Fluorescent molecule
CN200880115960.5A CN101896491B (zh) 2007-09-14 2008-08-01 荧光发生分子
EP08792084A EP2204371B1 (en) 2007-09-14 2008-08-01 Fluorescent molecule from a profluorophore

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007239234 2007-09-14
JP2007-239234 2007-09-14

Publications (1)

Publication Number Publication Date
WO2009034790A1 true WO2009034790A1 (ja) 2009-03-19

Family

ID=40451802

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063871 WO2009034790A1 (ja) 2007-09-14 2008-08-01 蛍光発生分子

Country Status (5)

Country Link
US (1) US8084201B2 (ja)
EP (1) EP2204371B1 (ja)
JP (1) JP5419278B2 (ja)
CN (1) CN101896491B (ja)
WO (1) WO2009034790A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011040612A1 (ja) 2009-10-01 2011-04-07 独立行政法人理化学研究所 蛍光発生分子および標的核酸検出方法
JP2012058169A (ja) * 2010-09-13 2012-03-22 Konica Minolta Holdings Inc プラズモン励起センサを用いるアッセイ方法及びプラズモン励起センサ
WO2012121396A1 (ja) * 2011-03-10 2012-09-13 独立行政法人理化学研究所 蛍光プローブ及びその利用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5773405B2 (ja) * 2010-07-09 2015-09-02 国立大学法人高知大学 細胞膜上分子と相互作用する化合物の検出方法
CN103937490B (zh) * 2014-04-24 2015-07-29 辽宁大学 一种检测线粒体中硫离子的荧光探针及其应用
EP3001197A1 (en) * 2014-09-23 2016-03-30 Universität Heidelberg Fluorescent protein stains
CN107337681B (zh) * 2017-07-28 2019-05-28 厦门大学 一种超氧阴离子探针及其制备方法和应用
CN112625049A (zh) * 2020-11-06 2021-04-09 上海应用技术大学 一种叠氮基团修饰荧光素类化合物及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001289851A (ja) * 2000-04-03 2001-10-19 Tokyo Inst Of Technol Ｄｎａの蛍光標識プローブ、蛍光標識プラスミド
US20040063918A1 (en) * 2002-07-16 2004-04-01 Young Travis G. Methods and materials for the synthesis of modified peptides
JP2005047898A (ja) * 2003-07-11 2005-02-24 Osaka Industrial Promotion Organization スルホン酸エステル化合物およびそれを用いた蛍光プローブ
JP2006500065A (ja) * 2002-09-25 2006-01-05 アマシャム・バイオサイエンス・コーポレイション エネルギー移動色素、ダイターミネーター及びその使用
WO2008075718A1 (ja) * 2006-12-19 2008-06-26 Riken 蛍光発生分子

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1260510B1 (en) * 2000-02-28 2006-10-18 Daiichi Pure Chemicals Co., Ltd. Fluorescent probes for the quantitation of zinc
CN1441792A (zh) * 2000-07-12 2003-09-10 Sk株式会社 油产品用静标记及其检测方法
US20050260593A1 (en) * 2002-09-25 2005-11-24 Shiv Kumar Fluorescent labeling reagents with multiple donors and acceptors
WO2005005401A1 (ja) * 2003-07-11 2005-01-20 Osaka Industrial Promotion Organization スルホン酸エステル化合物およびそれを用いた蛍光プローブ

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001289851A (ja) * 2000-04-03 2001-10-19 Tokyo Inst Of Technol Ｄｎａの蛍光標識プローブ、蛍光標識プラスミド
US20040063918A1 (en) * 2002-07-16 2004-04-01 Young Travis G. Methods and materials for the synthesis of modified peptides
JP2006500065A (ja) * 2002-09-25 2006-01-05 アマシャム・バイオサイエンス・コーポレイション エネルギー移動色素、ダイターミネーター及びその使用
JP2005047898A (ja) * 2003-07-11 2005-02-24 Osaka Industrial Promotion Organization スルホン酸エステル化合物およびそれを用いた蛍光プローブ
WO2008075718A1 (ja) * 2006-12-19 2008-06-26 Riken 蛍光発生分子

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011040612A1 (ja) 2009-10-01 2011-04-07 独立行政法人理化学研究所 蛍光発生分子および標的核酸検出方法
JP2012058169A (ja) * 2010-09-13 2012-03-22 Konica Minolta Holdings Inc プラズモン励起センサを用いるアッセイ方法及びプラズモン励起センサ
WO2012121396A1 (ja) * 2011-03-10 2012-09-13 独立行政法人理化学研究所 蛍光プローブ及びその利用

Also Published As

Publication number Publication date
CN101896491A (zh) 2010-11-24
US8084201B2 (en) 2011-12-27
JP5419278B2 (ja) 2014-02-19
US20110076680A1 (en) 2011-03-31
EP2204371A1 (en) 2010-07-07
JPWO2009034790A1 (ja) 2010-12-24
EP2204371A4 (en) 2010-11-24
EP2204371B1 (en) 2012-12-12
CN101896491B (zh) 2014-02-26

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