WO2009027544A1 - Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal. - Google Patents
Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal. Download PDFInfo
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- WO2009027544A1 WO2009027544A1 PCT/ES2007/000497 ES2007000497W WO2009027544A1 WO 2009027544 A1 WO2009027544 A1 WO 2009027544A1 ES 2007000497 W ES2007000497 W ES 2007000497W WO 2009027544 A1 WO2009027544 A1 WO 2009027544A1
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- biological fertilizer
- strains
- indole
- acetic acid
- fertilizer according
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Definitions
- New biological fertilizer obtaining procedure and its use as a plant growth stimulator.
- the present invention concerns a product for biological fertilization consisting of a granulated formulation comprising two strains of bacteria of the Azospirillum and Pantoea genera, with the ability to fix atmospheric nitrogen, solubilize phosphates as well as other mineral nutrients from the soil and produce high amounts of plant growth stimulating substances.
- Said microorganisms have been immobilized by means of the adsorption technique to a solid support, which acts as a slow release system, which also guarantees a high stability in the cell viability, as well as organic nutrients and sufficient salts to facilitate the colonization of the roots of the plants.
- the isolated microorganisms, the cell immobilization process, and the use of the biological fertilizer thus obtained as a plant growth stimulator are also objects of this invention.
- fertilizers are essential for the maintenance of high yields in crops.
- chemical fertilization significant amounts of nitrogen, phosphorus and potassium are added to the soil, as well as other mineral elements, however, their availability is very low, since it is well known that a fraction is immobilized in the soil forming compounds insoluble not assimilable by the plants and another one is washed by a leaching process, which in addition to economic losses, generates a major problem of environmental pollution.
- a group of microorganisms that have a remarkable importance in this phenomenon is one that participates in the solubilization of phosphorus from sources that would otherwise be inaccessible to plants. (Kucey et al, 1989).
- Phosphate solubilizing microorganisms have been isolated in virtually all tested soils, although the number and proportion of these varies according to the type of soil, climate and other factors such as the historical evolution of the soil. Many microorganisms are capable of assimilating the insoluble phosphorus of the soil, releasing a part of it in the form of soluble phosphates that in turn can be used by plants, thus contributing to plant nutrition (Chabot et al, 1993).
- This factor has two determining aspects, the durability of the product and its ability to colonize the roots of the crops to be stimulated or protected, once applied in the field. Some may arise problems when it comes to cells that do not have the capacity to form resistance structures, since the crops are losing viability over time and also their ability to survive in the soil is very low. For this reason, it is essential that the product is capable of preserving the cell viability in adverse conditions for long periods of time and guaranteeing as far as possible the ability to colonize the roots once applied in the field.
- One of the most important criteria to consider for this is to achieve preparations that consistently release an appreciable number of viable cells. For this purpose, cell immobilization techniques offer a series of advantages over free cells, which make them very attractive for application in practice and particularly for agricultural and environmental biotechnology.
- immobilized cells can enhance the effects of microorganisms without creating contamination problems, leading to very active and innovative products.
- a technique widely used in practice is adsorption. Numerous have been the supports used for this purpose. In agriculture in particular, the use of clay, vermiculite, perlite, sepiolite, kaolin, diatomaceous earth, natural zeolite and others is commonly used.
- Zeolites are aluminum silicates, whose networks are formed by tetrahedra of
- This trellis has a negative charge that is compensated with interchangeable cations that occupy specific sites in the channels and cavities of the zeolite.
- This salt has two very important properties; adsorption capacity and ion exchange, which are very advantageous for possible use as immobilization support.
- the properties of these products could be very attractive for obtaining a dry product for biofertilization, especially if they are microorganisms that are not capable of forming resistance structures. For this purpose, it would be essential to ensure that cell mortality is very low or zero during the production process and particularly in the drying stage of the product. Achieving this objective would allow to obtain very effective products whenever a production process is achieved.
- ES 2 234 417 describes a biological fertilizer consisting of a granular formulation containing the C 3 strain of the dispersed Pantoea species deposited in the Spanish Type Culture Collection (CECT) with the number CECT-5801 and the M 3 strain of the Azospirillum species Brazilian presented in the Spanish Type Culture Collection (CECT) with the number CECT-5802, both immobilized on a solid support and a process for its preparation.
- Said biological fertilizer is capable of fixing atmospheric nitrogen, as well as solubilizing phosphates and other mineral nutrients, resulting in good activity. fertilizer.
- This activity is due to the fact that, for example, the C 3 strain is capable of producing large amounts of organic acids, mainly gluconic acid, useful for the solubilization of the insoluble phosphate of the soil, while for example the M 3 strain is capable of fixing nitrogen and produce indole-3-acetic acid, a very important factor involved in the growth of plants.
- organic acids mainly gluconic acid
- M 3 strain is capable of fixing nitrogen and produce indole-3-acetic acid, a very important factor involved in the growth of plants.
- strains are immobilized on a solid support, for example zeolite, to obtain a final product characterized by good stability as well as satisfactory cell viability.
- the solid support is additionally supplied with salts, to facilitate the colonization of the roots of the plants.
- the fertilizer according to ES 2 234 417 when it contacts the plant through the ground needs some time to carry out its action, since for the strains to begin their activity they must colonize the roots of the plants and establish with them a relationship that allows them to perform their conversion action on a useful product.
- the fertilizer activity cannot be carried out immediately when the fertilizer contacts the plant through the soil, but it can be observed after a certain time, which depends on the characteristics of the soil, climatic conditions and many others. factors.
- This aspect can cause a delay of the biofertilizing action and some growth retardation, at least initially, in the treated plants. In fact, certain weather conditions such as rain, hail, etc. they may affect part or all of the soil fertilizer thus disabling its activity.
- the process for the preparation of a biological fertilizer includes the adsorption of the strains as well as the nutrients and salts in the solid support, thus obtaining a wet product, which needs to be dried to obtain a high cell stability In fact, a high level of moisture in the final product can cause rapid degradation of the strains and lower viability.
- the drying step is carried out at a temperature between 60-80 0 C, Ia which is relatively high for preserving the viability of the strains.
- the humidity level obtained in this way is between 3-6%.
- An object of the present invention is to provide a biological fertilizer that exerts a fertilizing and / or stimulating action of the immediate growth of the plants as soon as the fertilizer contacts the plant through the soil.
- Another object of the present invention is to provide a biological fertilizer that exerts an enhanced fertilizer and / or growth stimulation activity.
- Another object of the present invention is to provide a biological fertilizer whose activity is maintained, that is, it is kept stable under storage conditions, for a long period of time, at least two years.
- the present invention also aims to provide a process for the preparation of a biological fertilizer comprising a reduced number of stages compared to the process according to the state of the art and presenting various industrial advantages.
- a fertilizer comprising: a.- a pure culture of strain C 3 of the dispersed Pantoea species deposited in the Spanish Type Culture Collection (CECT) with the number CECT 5801 , b.- a pure culture of strain M 3 of the species Azospirillum brasilense deposited in Ia
- the new biological fertilizer according to the present invention contains cells of Azospirillum brasilense strain M 3 fixing atmospheric nitrogen and having high ability to produce substances regulating plant growth type indole-3-acetic acid (IAA), and Pantoea dispersed, strain C 3 that has a high efficiency in the production of organic acids, mainly gluconic acid, for the solubilization of phosphates and other nutrients of the soil, as well as the ability to produce for example siderophores and substances regulating plant growth. Said microorganisms have been deposited in the Spanish Type Culture Collection
- Said biological fertilizer consists of a product consisting of a solid support, in which the bacteria have been immobilized and which also contains the necessary nutrients to guarantee their survival, once the plants are inoculated, acting as a slow release system.
- the microorganism Azospirillum brasilense M 3 CECT 5802 was obtained by a procedure that combines the isolation in semi-solid NFb medium and the selection through its ability to stimulate plant growth and produce indole-3 acetic acid and other phytohormones.
- the ability to stimulate plant growth was verified by laboratory and greenhouse bioassays, according to the methods described by Bashan et al 1986, Fernández 1995 and Bashan 1998. It was proved, by these bioassays, that the strain M 3 was the one with the greatest stimulatory effect of the Growth produced more than 50 isolates of nitrogen fixing bacteria tested.
- AIA indole-3-acetic acid
- the Pantoea dispersed C 3 CECT 5801 microorganism was obtained using a procedure that combines the isolation in agarized media with Tris_HCI 1N buffer pH 8, by agar clearance zones (Gyaneshwar et al 1999) and selection by determination of solubilized PO 4 3 " in agitated liquid media (Nautiyal 1999), using in both cases insoluble Ca 3 PO 4 as the sole source of phosphorus, the characterization of the organic acids produced was carried out and it was proven that it produces mostly gluconic acid. through its ability to stimulate plant growth and produce auxins.
- the new biological fertilizer comprises, in addition to strains C 3 and M 3 , indole-3-acetic acid (AIA).
- AIA indole-3-acetic acid
- This third component represents significant progress with respect to known biological fertilizers.
- the presence of AIA from the beginning together with the two strains C 3 and M 3 gives the resulting product the possibility of being active immediately when the fertilizer contacts the plant through the soil, since the AIA is able to exert its action as a stimulator of plant growth, while strains C 3 and M 3 are able to start the production of AIA after inoculation in the soil and after the conversion of the necessary substrates.
- AIA acts as a stimulator of plant growth as soon as the fertilizer contacts the plant through the soil, thus allowing the C 3 and M 3 strains to colonize the roots of the plants and establish with them the relationship that allows them to perform their growth stimulating action.
- AIA indole-3-acetic acid
- both strains C 3 and M 3 are capable of producing ndol-3-acetic acid (AIA), thus giving the product a substantially improved activity as fertilizer and stimulator of the Plant growth compared to known biological fertilizers acting in similar amounts.
- AIA ndol-3-acetic acid
- the new biological fertilizer according to the present invention in comparison with the same amount of a fertilizer according to the state of the art, is capable of producing a greater amount of AIA.
- the AIA can be advantageously produced during the fermentation stage for the preparation of the strains, or added directly at the end of the fermentation process thereof before adsorption in the solid support Alternatively, the AIA can be added to the nutrients before their adsorption on the solid support.
- the formation of AIA can be induced through the addition of tryptophan to the culture medium during the fermentation stage for the preparation of the strains, allowing the production of AIA by them.
- the resulting culture broth includes strains (C 3 or M 3 ) and AIA that has been produced directly by the strains.
- the broth can be adsorbed directly on the solid support including the AIA produced during fermentation.
- the present invention provides a process for the production of biological fertilizers, said process comprises the following steps: a) Cultivate, C 3 and M 3 microorganisms. b) Immobilization in solid matrices. c) Drying.
- step c) consists of drying the solid matrix.
- This stage is the main method improved.
- the drying temperatures are 8O 0 C and 20O 0 C respectively.
- a high temperature is required to reduce the degree of moisture of the solid matrix, for example, zeolite.
- the acceptable degree of humidity should be between 4 and 8%, although a lower degree of humidity would be desirable.
- the limits and conditions mentioned above according to the state of the art are surprisingly exceeded by the method described in accordance with the present invention.
- the improvement in the drying is based fundamentally on the fact that in the present invention the drying is carried out in a fluidized bed dryer that allows the temperature of the drying air to be significantly reduced since the contact area increases markedly.
- the drying stage is thus characterized in that it comprises the dehydration of the solid support, adsorbed with the microorganisms and / or nutrients and salts, carried out in a fluidized bed dryer allowing to significantly reduce the residual moisture of the product operating at a temperature of only 35 0 C.
- Figure 1 shows the results of fertilization treatments in plants, obtained with the biological fertilizer according to the present invention.
- figures 2, 3 and 4 show a summary of the results obtained in the different tests carried out, regarding the average weight of the lettuce and the nitrate content in the soils and leaves.
- the fluidized bed dryer is fed with the mass necessary for its operation and it is worked at a maximum air temperature of 35 0 C. In this way the product will dry until it reaches a final humidity that will be between 2-5%, preferably 2-3%, more preferably 2-2.5% which is the moisture value of the finished product.
- the introduction of L-tryptophan in the culture medium causes high amounts of auxins to be produced during fermentation, which then pass to the final product, giving it an additional rooting effect from its application.
- the culture medium is very economical and what is more important, high cell counts of the order of 10 9 -10 10 cells x ml_ "1 are reached in both cases. This medium has It has also been tested with similar results in the cultivation of ⁇ e strains and other bacteria and fungi isolated by the authors.
- L-tryptophan in the growth medium of the fermentation step provides an immediate source for the production of AIA by the microorganisms C 3 and M 3 .
- stage b) has another advantage that consists in the immobilization in the same solid matrix of cells, salts and nutrients.
- This process is carried out simultaneously and by atomization of the solid, with the fermentation broths and nutrient solutions, following a sequential scheme.
- a solid mixer is used, which has an accessory that allows to spray liquids as it mixes and homogenizes.
- the mixer is charged with the zeolite and the phosphoric rock and the mixing process is carried out for 5-10 minutes.
- the cell broths are added in sequential order, first M 3 and then C 3 .
- the process of adsorption of nutrients is carried out using the following raw materials and proportions:
- each of the solutions described above is sprayed sequentially and in the same order in which they are described. Mix well for 15-30 minutes to obtain a very homogeneous moist solid.
- This new improved stage reduces the process time and allows obtaining a solid matrix containing the components of the invention.
- This advantage is very important, since the solid support can be subjected to a single drying stage, thus avoiding the need to carry out at least three different drying stages for the different solid supports with different components.
- the main advantage of the process of obtaining, in accordance with the present invention is the new drying stage.
- This new drying stage could be carried out on solid supports according to the prior art, as well as solid supports obtained according to a new aspect, that is, the possibility of immobilizing both strains C 3 and M 3 as well as nutrients and any another component in the solid matrix at the same time and in a single stage of the process.
- the biological fertilizer can be produced in accordance with any process described herein, just as the new process can be used for the production of any product of the class described above.
- Example 1 Propagation of strains Azospir ⁇ llum brasilense M 3 and Pantoea dispersa C 3
- strain C 3 followed the same fermentation scheme.
- the purity of the culture was checked in MacConkey medium (OXOID 1981) and the inoculum was incubated in an orbital shaker for 12 hours.
- the cultivation in the BIOSTAT ® D fermenter was carried out for 24-36 hours at a stirring speed of 300 rpm, and aeration of 100 L x min "1 (0.5 vvm) and a temperature of 30 0 C.
- the pH is allowed to vary freely too and in the end it had a value of 6.7.
- a concentration ⁇ e ⁇ , ⁇ ⁇ x 10 10 cel x ml_ "1 was reached and a final concentration of AIA was 50 mg x L " 1 .
- Example 2 Cellular immobilization and nutrient loading
- a plow mixer that has a sprinkler and the following raw materials are used:
- the mixer is charged with the zeolite and the phosphoric rock and the mixing process is carried out for 5-10 minutes.
- the cell broths are atomized in sequential order, first M 3 and then C 3 .
- the process of adsorption of nutrients is carried out using the following raw materials and proportions:
- each of the solutions described above is sprayed sequentially and in the same order in which they are described, passing a little water between each nutrient to clean the ducts.
- 7.2 L were added in this phase. Mix well for 15-30 minutes to obtain a very homogeneous moist solid.
- the zeolite with the phosphoric rock was humidified with 14.4 L of total liquid, which was completely absorbed without excess liquid remaining.
- the fluidized bed dryer is fed with the mass necessary for its operation and it is worked at a maximum air temperature of 35 0 C. In this way the product will dry until it reaches a final humidity that will be between 2-3% that is the moisture value of the finished product
- this figure demonstrates that the lettuce, treated with the biological fertilizer with more than two years of conservation, maintains a higher standard of natural growth compared to the control that has not received any treatment.
- the gray bar of the graph represents the fresh weight of the green part of plants (PFV) and the black bar indicates the fresh weight of the root (PFR) of lettuces resulting from a bioassay in greenhouse in pots and on peat as substrate.
- the bar chart shows that the fertilizer, obtained by this procedure, keeps higher stability and activity for more than two years. As can be observed, over two and a half years, the product still retains more than 90% of its effectiveness.
- Example 4 Evaluation of agricultural advantages. To demonstrate that the product is more active compared to chemical fertilizers, several experimental conditions have been investigated. For example, it was tested in field conditions in different crops and the results obtained have been very satisfactory. Particularly in the cultivation of lettuce, it was tested in field conditions looking for the variability between soils, varieties and irrigation systems.
- Figure 2 shows the effects of the treatment on the growth of the plant, measured as average weight in grams.
- the gray bar of the graph represents the biological fertilizer according to the present invention, and the black bar indicates the control to use. It is observed that in particular experimental conditions the weight of the lettuce treated with the fertilizer is greater than or comparable to that of the lettuce treated with chemical fertilizer. In fact, it is observed that despite not using chemical fertilization, the results obtained are similar and even superior to those of the crop in use.
- Figures 3 and 4 show a decrease in concentration ⁇ e miraros and environmental pollutants in the soil (figure 3) and in the leaves (figure 4). It is evident that the use of biological fertilizer significantly reduces the presence of nitrates and / or contaminants both in the soil and in the leaves, compared to chemical fertilizers.
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Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2007801013030A CN101842334B (zh) | 2007-08-27 | 2007-08-27 | 新型生物肥料、获得所述肥料的方法及所述肥料作为植物生长刺激剂的应用 |
US12/675,863 US8242053B2 (en) | 2007-08-27 | 2007-08-27 | Biological fertilizer, method for obtaining same and use thereof as a plant growth stimulator |
AU2007358190A AU2007358190B2 (en) | 2007-08-27 | 2007-08-27 | Novel biological fertiliser, method for obtaining same and use thereof as a plant growth stimulator |
PCT/ES2007/000497 WO2009027544A1 (es) | 2007-08-27 | 2007-08-27 | Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal. |
PT78229051T PT2194032E (pt) | 2007-08-27 | 2007-08-27 | Novo fertilizante biológico, método para obtenção do mesmo e sua utilização como estimulador do crescimento de plantas |
ES07822905.1T ES2551678T3 (es) | 2007-08-27 | 2007-08-27 | Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal |
PL07822905T PL2194032T3 (pl) | 2007-08-27 | 2007-08-27 | Nowy nawóz biologiczny, sposób jego otrzymywania i jego zastosowanie jako stymulatora wzrostu roślin |
EP07822905.1A EP2194032B1 (en) | 2007-08-27 | 2007-08-27 | Novel biological fertiliser, method for obtaining same and use thereof as a plant growth stimulator |
BRPI0722102-9A2A BRPI0722102A2 (pt) | 2007-08-27 | 2007-08-27 | Novo fertilizante biológico, método para obtenção do mesmo e utilização deste como estimulador do crescimento vegetal |
CY20151101045T CY1117255T1 (el) | 2007-08-27 | 2015-11-19 | Νεο βιολογικο λιπασμα, μεθοδος για την αποκτηση του και η χρηση του ως διεγερτη αυξησης των φυτων |
Applications Claiming Priority (1)
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PCT/ES2007/000497 WO2009027544A1 (es) | 2007-08-27 | 2007-08-27 | Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal. |
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WO2009027544A1 true WO2009027544A1 (es) | 2009-03-05 |
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PCT/ES2007/000497 WO2009027544A1 (es) | 2007-08-27 | 2007-08-27 | Nuevo fertilizante biológico, procedimiento de obtención y su uso como estimulador del crecimiento vegetal. |
Country Status (10)
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US (1) | US8242053B2 (es) |
EP (1) | EP2194032B1 (es) |
CN (1) | CN101842334B (es) |
AU (1) | AU2007358190B2 (es) |
BR (1) | BRPI0722102A2 (es) |
CY (1) | CY1117255T1 (es) |
ES (1) | ES2551678T3 (es) |
PL (1) | PL2194032T3 (es) |
PT (1) | PT2194032E (es) |
WO (1) | WO2009027544A1 (es) |
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WO2011121408A1 (en) | 2010-03-31 | 2011-10-06 | Probelte, Sa | Bacterial strains and a bionematicide and plant growth stimulator containing them |
IT201800002826A1 (it) * | 2018-02-19 | 2019-08-19 | Bal Co S P A | Procedimento per la preparazione di una composizione stimolante per superfici vegetali e terreni |
WO2020216978A1 (es) | 2019-04-26 | 2020-10-29 | Probelte S.A.U. | Biofertilizante líquido que comprende cepas de azospirillum brasilense y pantoea dispersa y método de obtención del mismo |
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US20180029945A1 (en) * | 2015-01-29 | 2018-02-01 | José Luis Miranda Valencia | Solid Granulated Fertilizer Formulated with Mineral Clays, Siderophore Chelating Agents, Secondary Nutrients and Micronutrients |
EP3880633A4 (en) | 2018-11-14 | 2022-08-17 | Anuvia Plant Nutrients Holdings Inc. | ADMINISTRATION OF BIOACTIVE MOLECULES IN COATINGS OR SURFACE LAYERS OF ORGANIC FORTIFIED INORGANIC FERTILIZER |
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CN114164140B (zh) * | 2021-10-28 | 2023-12-12 | 中国林业科学研究院华北林业实验中心 | 一株高效溶磷菌mqr6及其发酵产物与应用 |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US570813A (en) | 1896-11-03 | Friedrich jstobbe and lorenz hiltner | ||
US1212196A (en) | 1916-10-28 | 1917-01-16 | Henry King Hannah | Fertilizer composition and process of making same. |
US4670037A (en) | 1985-08-12 | 1987-06-02 | Kistner Sr Harold E | Fertilizer utilizing a chitin-producing fungus and method for its use |
US5366532A (en) | 1987-02-27 | 1994-11-22 | Lipha, Lyonnaise Industrielle | Fertilizers containing microorganisms and their production processes |
ES2093559A1 (es) | 1995-05-04 | 1996-12-16 | Consejo Superior Investigacion | Fertilizante bacteriano y procedimiento de obtencion. |
US5951978A (en) | 1998-12-10 | 1999-09-14 | Tatko Biotech, Inc. | Microorganisms for improving plant productivity |
US6596273B2 (en) | 2001-03-01 | 2003-07-22 | Ultra Biotech Limited | Biological fertilizer compositions comprising swine manure |
ES2234417A1 (es) | 2003-10-24 | 2005-06-16 | Probelte S.A. | Nuevo fertilizante biologico y procedimiento de obtencion. |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060178269A1 (en) * | 2005-02-08 | 2006-08-10 | Medina-Vega Luis R | Plant conditioning treatment for plant growth and health enhancement |
-
2007
- 2007-08-27 WO PCT/ES2007/000497 patent/WO2009027544A1/es active Application Filing
- 2007-08-27 PL PL07822905T patent/PL2194032T3/pl unknown
- 2007-08-27 US US12/675,863 patent/US8242053B2/en not_active Expired - Fee Related
- 2007-08-27 BR BRPI0722102-9A2A patent/BRPI0722102A2/pt not_active Application Discontinuation
- 2007-08-27 PT PT78229051T patent/PT2194032E/pt unknown
- 2007-08-27 EP EP07822905.1A patent/EP2194032B1/en active Active
- 2007-08-27 AU AU2007358190A patent/AU2007358190B2/en not_active Ceased
- 2007-08-27 CN CN2007801013030A patent/CN101842334B/zh not_active Expired - Fee Related
- 2007-08-27 ES ES07822905.1T patent/ES2551678T3/es active Active
-
2015
- 2015-11-19 CY CY20151101045T patent/CY1117255T1/el unknown
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US570813A (en) | 1896-11-03 | Friedrich jstobbe and lorenz hiltner | ||
US1212196A (en) | 1916-10-28 | 1917-01-16 | Henry King Hannah | Fertilizer composition and process of making same. |
US4670037A (en) | 1985-08-12 | 1987-06-02 | Kistner Sr Harold E | Fertilizer utilizing a chitin-producing fungus and method for its use |
US5366532A (en) | 1987-02-27 | 1994-11-22 | Lipha, Lyonnaise Industrielle | Fertilizers containing microorganisms and their production processes |
ES2093559A1 (es) | 1995-05-04 | 1996-12-16 | Consejo Superior Investigacion | Fertilizante bacteriano y procedimiento de obtencion. |
US5951978A (en) | 1998-12-10 | 1999-09-14 | Tatko Biotech, Inc. | Microorganisms for improving plant productivity |
US6596273B2 (en) | 2001-03-01 | 2003-07-22 | Ultra Biotech Limited | Biological fertilizer compositions comprising swine manure |
ES2234417A1 (es) | 2003-10-24 | 2005-06-16 | Probelte S.A. | Nuevo fertilizante biologico y procedimiento de obtencion. |
Non-Patent Citations (2)
Title |
---|
BRILL, W.F., ATLAS AND BARTHA, 1977 |
See also references of EP2194032A4 |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011121408A1 (en) | 2010-03-31 | 2011-10-06 | Probelte, Sa | Bacterial strains and a bionematicide and plant growth stimulator containing them |
ES2378040A1 (es) * | 2010-03-31 | 2012-04-04 | Probelte, S.A | Un preparado biológico bionematicida y estimulador del crecimiento vegetal y cultivos puros de las cepas denominadas n11, sr11 y alo1, contenidas en el mismo. |
CN101967461A (zh) * | 2010-10-22 | 2011-02-09 | 秦皇岛领先科技发展有限公司 | 一种固氮螺菌的发酵方法与由该方法得到的发酵液及其用途 |
CN101967461B (zh) * | 2010-10-22 | 2012-06-27 | 领先生物农业股份有限公司 | 一种固氮螺菌的发酵方法与由该方法得到的发酵液及其用途 |
IT201800002826A1 (it) * | 2018-02-19 | 2019-08-19 | Bal Co S P A | Procedimento per la preparazione di una composizione stimolante per superfici vegetali e terreni |
WO2019159148A1 (en) * | 2018-02-19 | 2019-08-22 | BAL-CO S.p.A. | Process for the preparation of a stimulating composition for vegetal surfaces and soils |
WO2020216978A1 (es) | 2019-04-26 | 2020-10-29 | Probelte S.A.U. | Biofertilizante líquido que comprende cepas de azospirillum brasilense y pantoea dispersa y método de obtención del mismo |
Also Published As
Publication number | Publication date |
---|---|
CY1117255T1 (el) | 2017-04-26 |
PT2194032E (pt) | 2015-11-25 |
CN101842334A (zh) | 2010-09-22 |
EP2194032A4 (en) | 2013-02-27 |
AU2007358190B2 (en) | 2013-09-12 |
PL2194032T3 (pl) | 2016-04-29 |
EP2194032A1 (en) | 2010-06-09 |
EP2194032B1 (en) | 2015-08-19 |
BRPI0722102A2 (pt) | 2014-04-08 |
ES2551678T3 (es) | 2015-11-23 |
AU2007358190A1 (en) | 2009-03-05 |
US20110045976A1 (en) | 2011-02-24 |
CN101842334B (zh) | 2013-11-20 |
US8242053B2 (en) | 2012-08-14 |
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