WO2019159148A1 - Process for the preparation of a stimulating composition for vegetal surfaces and soils - Google Patents

Process for the preparation of a stimulating composition for vegetal surfaces and soils Download PDF

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Publication number
WO2019159148A1
WO2019159148A1 PCT/IB2019/051297 IB2019051297W WO2019159148A1 WO 2019159148 A1 WO2019159148 A1 WO 2019159148A1 IB 2019051297 W IB2019051297 W IB 2019051297W WO 2019159148 A1 WO2019159148 A1 WO 2019159148A1
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WIPO (PCT)
Prior art keywords
composition
fact
process according
microorganisms
mixture
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Application number
PCT/IB2019/051297
Other languages
French (fr)
Inventor
Domenico PRISA
Original Assignee
BAL-CO S.p.A.
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Publication of WO2019159148A1 publication Critical patent/WO2019159148A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
    • A01N25/28—Microcapsules or nanocapsules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces

Definitions

  • the present invention relates to a process for the preparation of a stimulating composition for vegetal surfaces and soils.
  • stimulating compositions applied to vegetal surfaces such as plants, seeds and growth substrates is well known, in order to modify their physiological growth processes, promoting the latter and improving their development and/or response to stress.
  • the known stimulating compositions specifically consist of substances and/or microorganisms applied to these surfaces and adapted to promote natural metabolic processes, improving the efficiency of absorption and assimilation of nutrients, tolerance to abiotic stress and product quality.
  • the expression “stimulating composition” refers indiscriminately to a fertilizer, corroborating, or bio stimulating composition.
  • bio-stimulants With particular reference to bio-stimulants, the latter are generally classified into three categories consisting of humic acids, products containing hormones and products containing amino acids.
  • Humic bio- stimulants consist of products containing substances derived from the decomposition of organic material and from the metabolic activity of predefined microbial consortia.
  • products containing hormones or amino acids consist of mixtures of soluble amino acids and peptides, generally obtained by chemical, enzymatic or animal or vegetal hydrolysis.
  • stimulating compositions consisting of microorganisms, directly applied on the surfaces of interest.
  • the dosage of the aforementioned compositions consisting of microorganisms is extremely complex and imprecise compared to real needs, resulting in a huge waste of material.
  • known stimulating compositions have high production and sales costs determined by the components of the same, the presence of which in the formulation is closely linked to the individual function that each product performs on the vegetal surface.
  • each stimulating composition is manufactured and used to perform a single function on the vegetal surface of application, without however achieving synergistic effects with the environment surrounding the surface itself, often resulting ineffective.
  • compositions and related preparation processes are known from patent documents n. WO2009/027544, EP0223662.
  • compositions and related preparation processes are known from the documents Jasna Hrenovic et ah:“Zeolited tuff as a carrier of bacteria” and In Hwa Kim et ah:“Development of a Microbe-Zeolite Carrier for the Effective Elimination of Heavy Metals from Seawater”.
  • the main aim of the present invention is to devise a process for the preparation of a stimulating composition for vegetal surfaces and soils which allows significantly increasing effectiveness compared to known stimulating compositions, performing synergistic effects with the environment surrounding the vegetal surface of application.
  • one object of the present invention is to create a process for the preparation of a stimulating composition for vegetal surfaces and soils that allows significantly reducing the costs of production and sale compared to known compositions.
  • the process comprises one step of supplying a basic composition comprising zeolite.
  • the expression“basic composition” refers to a starting composition comprising zeolite.
  • the basic composition consists of zeolite.
  • the zeolite is chabasite zeolite.
  • the basic composition comprises zeolitites.
  • the basic composition consists of zeolitites.
  • the basic composition comprises kaolin.
  • kaolin is present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, higher than 30%.
  • the kaolin is present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, substantially equal to 50%.
  • the basic composition consists of a mixture comprising zeolite and kaolin respectively present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, substantially equal to 50%.
  • the process comprises one drying step of the basic composition at a work temperature higher than 80°C to obtain a sterili ed composition.
  • the work temperature is lower than l40°C.
  • the work temperature is comprised between 90°C and l70°C.
  • the drying step is adapted to determine the killing of the autochthonous microorganisms present in the basic composition.
  • the drying step is carried out by means of a rotary dryer of a type known to the expert in the field.
  • the process comprises a splitting step of the sterilized composition into a first fraction and into a second fraction.
  • the process comprises a preparation step for a mixture of microorganisms.
  • the mixture of microorganisms comprises at least two microorganisms belonging to the kind chosen from the list comprising: Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria.
  • Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria can comprise two, three, four, etc. microorganisms belonging respectively to a kind chosen from the list comprising: Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria.
  • the mixture of microorganisms can comprise one microorganism belonging to the kind Bacillus, one microorganism belonging to the kind Pseudomonas, one microorganism belonging to the kind Lactobacillus, one microorganism belonging to the kind Streptomyches, one microorganism belonging to the kind Aspergillus, one microorganism belonging to the kind Trichoderma and one microorganism belonging to the kind Cyanobacteria.
  • the mixture of microorganisms consists of one microorganism belonging to the kind Bacillus, one microorganism belonging to the kind Pseudomonas, one microorganism belonging to the kind Lactobacillus, one microorganism belonging to the kind Streptomyches, one microorganism belonging to the kind Aspergillus, one microorganism belonging to the kind Trichoderma, one microorganism belonging to the kind Cyanobacteria, and one microorganism belonging to the kind Rhodobacter.
  • the microorganisms of the kind Bacillus comprise the species Bacillus subtilis and Bacillus amyloliquefaciens
  • the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum.
  • each microorganism is present in a concentration equal to 10 2 - 10 8 UFC/ml.
  • each microorganism is present in a concentration substantially equal to 10 6 UFC/ml.
  • the mixture of microorganisms consists of a microorganism belonging to the kind Pseudomonas sp., of two microorganisms belonging to the kind Bacillus.
  • the two microorganisms of the kind Bacillus belong to the species Bacillus subtilis and to the species Bacillus amyloliquefaciens respectively.
  • This mixture of microorganisms has the following formulation:
  • the mixture of microorganisms consists of a microorganism belonging to the kind Pseudomonas sp., of one microorganism belonging to the kind Bacillus, and of one microorganism belonging to the kind Trichoderma.
  • the microorganism of the kind Bacillus belongs to the species Bacillus subtilis
  • the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum sp.
  • This mixture of microorganisms has the following formulation: Trichoderma harzianum 10 6 UFC/ml
  • the mixture of microorganisms consists of a microorganism belonging to the kind Trichoderma and of two microorganisms belonging to the kind Bacillus.
  • the microorganisms of the kind Bacillus belong to the species Bacillus subtilis and to the species Bacillus amyloliquefaciens respectively
  • the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum sp.
  • This mixture of microorganisms has the following formulation:
  • the process comprises one fermentation step of the mixture of microorganisms at an operating temperature higher than 25°C to obtain a fermented mixture of microorganisms.
  • the aforementioned fermentation step is carried out by means of a fermenter of the type known to those skilled in the art.
  • the fermentation step takes place at an operating temperature comprised between 30°C and 40°C.
  • the fermentation step lasts between 7 and 10 days.
  • the process comprises a first incubation step of the fermented mixture of microorganisms at a temperature comprised between l5°C and 45°C.
  • the first incubation step takes place at a temperature comprised between 20°C and 40°C.
  • the first incubation step consists in diluting 1 L of the fermented mixture of microorganisms in 50 L of water to obtain a final solution.
  • the process comprises an inoculation step of the mixture of microorganisms, specifically of the fermented mixture of microorganisms, with the first fraction of the sterilized composition to obtain a colonized composition.
  • the inoculation step comprises a dispersion step of the fermented mixture of microorganisms in an aqueous solvent to obtain a suspension.
  • the process comprises an application step of the suspension onto the first fraction.
  • this suspension is applied onto the first fraction of the sterilized composition by simple spreading of the same, or alternatively by means of a spray dispenser to obtain the colonized composition.
  • the process continues comprising a mixing step of the colonized composition in a percentage by weight comprised between 0.1% and 4% with the second fraction of the sterilized composition to obtain a final composition.
  • the mixing step consists in joining together a percentage by weight substantially equal to 1% of the colonized composition with a predefined quantity of the sterilized composition, in detail the second fraction.
  • the colonized composition combined with the sterili ed composition determines the colonization of the latter too, creating a photo-protective film evenly distributed on the final composition.
  • the process comprises a second incubation step of the final composition for an operating time substantially equal to 5 days and at an operating temperature comprised between 20°C and 30°C.
  • the operating temperature is preferably comprised between 22°C and 26°C.
  • the process comprises an auxiliary drying step adapted to reduce the moisture content present in the final composition.
  • the presence of microorganisms applied on the surface of the sterilized composition creates a film with thermo-protective action ensuring that the chemical-physical conditions of reproduction of the microorganisms themselves remain unchanged over time.
  • the fact of providing the basic composition consisting of sterili ed zeolite eliminates the phenomena of microbial competition between the autochthonous microorganisms physiologically present in the zeolite and the mixture of microorganisms applied.
  • the process comprises a fragmentation step of the final composition to obtain a predefined particle size depending on the use of the latter, i.e. of the vegetal surface, or of the type of soil on which it is applied. It is specified that in the present treatise the expression“fragmentation step” means all the operations aimed at determining a dimensional reduction of the final composition, i.e. aimed at reducing the latter to a minimum until it reaches parts of the order of micrometric size.
  • fragmentation step also refers to the micronization of the final composition.
  • the particle size of the final composition is less than 10 mm.
  • the particle size of the final composition is comprised between 3 and 6 mm.
  • the above-mentioned particle sizes are used for the application of the composition on soils, potted plants, in open fields, or vegetable plants such as e.g. hazel, olive, vines.
  • the particle size of the final composition is less than 20 pm.
  • the particle size of the final composition is less than 10 pm.
  • the final composition having the aforementioned particle size is used on vegetal surfaces of the type of leaves, seeds, or roots.
  • the present invention also relates to the stimulating composition obtained from the previously described process.
  • the term“soil” relates to the surface layer covering the earth's crust. It has in practice been ascertained that the described invention achieves the intended objects.
  • the fact of applying the mixture of microorganisms on the sterilized composition allows creating a protective film against light, temperature variations and the action of bacteria and fungi.
  • crystalline structure of zeolites and zeolitites characterized by cavities and structural channels in their natural state occupied by compensating cations and water molecules allows, in accordance with the present invention, use for inoculation and growth of the mixture of microorganisms.
  • the crystalline structure of the zeolite allows maintaining the chemical-physical conditions for the growth of the mixture of microorganisms, ensuring their effectiveness over time.
  • the fact of providing for the fragmentation of the colonized composition increases the surface/volume ratio of the stimulating composition with respect to the surface of application, significantly increasing its effectiveness.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Organic Chemistry (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The process for the preparation of a stimulating composition for vegetal surfaces and soils comprises the steps of: supplying at least one basic composition comprising zeolite; inoculating at least one mixture of microorganisms with a first fraction of the basic composition to obtain a colonized composition.

Description

PROCESS FOR THE PREPARATION OF A STIMULATING COMPOSITION FOR VEGETAL SURFACES AND SOILS
Technical Field
The present invention relates to a process for the preparation of a stimulating composition for vegetal surfaces and soils.
Background Art
The use of stimulating compositions applied to vegetal surfaces such as plants, seeds and growth substrates is well known, in order to modify their physiological growth processes, promoting the latter and improving their development and/or response to stress.
The known stimulating compositions specifically consist of substances and/or microorganisms applied to these surfaces and adapted to promote natural metabolic processes, improving the efficiency of absorption and assimilation of nutrients, tolerance to abiotic stress and product quality.
It is specified that in the present treatise, the expression “stimulating composition” refers indiscriminately to a fertilizer, corroborating, or bio stimulating composition.
With particular reference to bio-stimulants, the latter are generally classified into three categories consisting of humic acids, products containing hormones and products containing amino acids.
Humic bio- stimulants consist of products containing substances derived from the decomposition of organic material and from the metabolic activity of predefined microbial consortia.
At the same time, products containing hormones or amino acids consist of mixtures of soluble amino acids and peptides, generally obtained by chemical, enzymatic or animal or vegetal hydrolysis.
Alternatively, the use is known of stimulating compositions consisting of microorganisms, directly applied on the surfaces of interest.
Known stimulating compositions, and in particular bio-stimulants, do however have numerous drawbacks linked to the difficulty of dosing the latter and their consequences in environmental terms.
In fact, the dosage of the aforementioned compositions consisting of microorganisms is extremely complex and imprecise compared to real needs, resulting in a huge waste of material.
In addition, the direct application of microorganisms to the vegetal surface of interest triggers uncontrolled microbial competition phenomena between autochthonous and applied microorganisms, significantly reducing the effectiveness of the bio -stimulants themselves.
At the same time, the prolonged use of stimulating compositions consisting of hormones and amino acids considerably increases the risk of environmental and, consequently, of plant product poisoning.
Furthermore, known stimulating compositions have high production and sales costs determined by the components of the same, the presence of which in the formulation is closely linked to the individual function that each product performs on the vegetal surface.
In fact, each stimulating composition is manufactured and used to perform a single function on the vegetal surface of application, without however achieving synergistic effects with the environment surrounding the surface itself, often resulting ineffective.
Other compositions and related preparation processes are known from patent documents n. WO2009/027544, EP0223662.
Again, further compositions and related preparation processes are known from the documents Jasna Hrenovic et ah:“Zeolited tuff as a carrier of bacteria” and In Hwa Kim et ah:“Development of a Microbe-Zeolite Carrier for the Effective Elimination of Heavy Metals from Seawater”.
Description of the Invention
The main aim of the present invention is to devise a process for the preparation of a stimulating composition for vegetal surfaces and soils which allows significantly increasing effectiveness compared to known stimulating compositions, performing synergistic effects with the environment surrounding the vegetal surface of application.
Within the illustrated aim, one object of the present invention is to create a process for the preparation of a stimulating composition for vegetal surfaces and soils that allows significantly reducing the costs of production and sale compared to known compositions.
The aforementioned objects are achieved by the process for the preparation of a stimulating composition for vegetal surfaces and soils, having the characteristics of claim 1.
Other characteristics and advantages of the present invention will be more evident from the description of a preferred, but not exclusive, embodiment of a process for the preparation of a stimulating composition for vegetal surfaces and soils.
Embodiments of the Invention
In the present description reference is made to a process for the preparation of a stimulating composition for vegetal surfaces and soils.
The process comprises one step of supplying a basic composition comprising zeolite.
It is specified that, in the present treatise, the expression“basic composition” refers to a starting composition comprising zeolite.
Alternatively, the basic composition consists of zeolite.
Advantageously, the zeolite is chabasite zeolite.
Preferably, the basic composition comprises zeolitites.
Alternatively, the basic composition consists of zeolitites.
According to a preferred embodiment, the basic composition comprises kaolin. Preferably, kaolin is present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, higher than 30%.
Specifically, the kaolin is present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, substantially equal to 50%.
Even more specifically, the basic composition consists of a mixture comprising zeolite and kaolin respectively present in a concentration by weight, evaluated with respect to the overall weight of the basic composition, substantially equal to 50%.
It should be noticed that the presence of kaolin considerably increases the functional value of the composition obtained by the process in accordance with the present invention, in particular due to its peculiar photo -protective properties.
According to a preferred embodiment, the process comprises one drying step of the basic composition at a work temperature higher than 80°C to obtain a sterili ed composition.
Advantageously, the work temperature is lower than l40°C.
Preferably, the work temperature is comprised between 90°C and l70°C.
The drying step is adapted to determine the killing of the autochthonous microorganisms present in the basic composition.
More in detail, the drying step is carried out by means of a rotary dryer of a type known to the expert in the field.
An alternative embodiment of the process according to the invention cannot however be ruled out, wherein the latter does not provide for the drying step. Subsequently, the process comprises a splitting step of the sterilized composition into a first fraction and into a second fraction.
At the same time, the process comprises a preparation step for a mixture of microorganisms.
The mixture of microorganisms comprises at least two microorganisms belonging to the kind chosen from the list comprising: Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria. This means that the mixture of microorganisms can comprise two, three, four, etc. microorganisms belonging respectively to a kind chosen from the list comprising: Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria.
For example, the mixture of microorganisms can comprise one microorganism belonging to the kind Bacillus, one microorganism belonging to the kind Pseudomonas, one microorganism belonging to the kind Lactobacillus, one microorganism belonging to the kind Streptomyches, one microorganism belonging to the kind Aspergillus, one microorganism belonging to the kind Trichoderma and one microorganism belonging to the kind Cyanobacteria.
It cannot be ruled out that the mixture of microorganisms consists of one microorganism belonging to the kind Bacillus, one microorganism belonging to the kind Pseudomonas, one microorganism belonging to the kind Lactobacillus, one microorganism belonging to the kind Streptomyches, one microorganism belonging to the kind Aspergillus, one microorganism belonging to the kind Trichoderma, one microorganism belonging to the kind Cyanobacteria, and one microorganism belonging to the kind Rhodobacter.
For example, the microorganisms of the kind Bacillus comprise the species Bacillus subtilis and Bacillus amyloliquefaciens, the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum.
Advantageously, each microorganism is present in a concentration equal to 102- 108 UFC/ml.
Preferably, each microorganism is present in a concentration substantially equal to 106 UFC/ml.
Below are some examples of the mixture of microorganisms made in accordance with the present invention.
EXAMPLE 1
The mixture of microorganisms consists of a microorganism belonging to the kind Pseudomonas sp., of two microorganisms belonging to the kind Bacillus. Specifically, the two microorganisms of the kind Bacillus belong to the species Bacillus subtilis and to the species Bacillus amyloliquefaciens respectively.
This mixture of microorganisms has the following formulation:
Pseudomonas sp. 106 UFC/ml
Bacillus subtilis 104 UFC/ml
Bacillus amyloliquefaciens 105 UFC/ml
EXAMPLE 2
The mixture of microorganisms consists of a microorganism belonging to the kind Pseudomonas sp., of one microorganism belonging to the kind Bacillus, and of one microorganism belonging to the kind Trichoderma.
Specifically, the microorganism of the kind Bacillus belongs to the species Bacillus subtilis, and the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum sp.
This mixture of microorganisms has the following formulation: Trichoderma harzianum 106 UFC/ml
Pseudomonas sp. 106 UFC/ml
Bacillus subtilis 106 UFC/ml EXAMPLE 3
The mixture of microorganisms consists of a microorganism belonging to the kind Trichoderma and of two microorganisms belonging to the kind Bacillus. Specifically, the microorganisms of the kind Bacillus belong to the species Bacillus subtilis and to the species Bacillus amyloliquefaciens respectively, and the microorganism of the kind Trichoderma belongs to the species Trichoderma harzianum sp.
This mixture of microorganisms has the following formulation:
Bacillus subtilis 106 UFC/ml
Bacillus amyloliquefaciens 105 UFC/ml
- Trichoderma harzianum sp 104 UFC/ml
Furthermore, the process comprises one fermentation step of the mixture of microorganisms at an operating temperature higher than 25°C to obtain a fermented mixture of microorganisms.
The aforementioned fermentation step is carried out by means of a fermenter of the type known to those skilled in the art.
Preferably, the fermentation step takes place at an operating temperature comprised between 30°C and 40°C.
Preferably, the fermentation step lasts between 7 and 10 days.
At the end of the fermentation step, the process comprises a first incubation step of the fermented mixture of microorganisms at a temperature comprised between l5°C and 45°C.
Preferably, the first incubation step takes place at a temperature comprised between 20°C and 40°C.
For example, the first incubation step consists in diluting 1 L of the fermented mixture of microorganisms in 50 L of water to obtain a final solution.
At this point, the process comprises an inoculation step of the mixture of microorganisms, specifically of the fermented mixture of microorganisms, with the first fraction of the sterilized composition to obtain a colonized composition. More in detail, the inoculation step comprises a dispersion step of the fermented mixture of microorganisms in an aqueous solvent to obtain a suspension.
In this regard, the process comprises an application step of the suspension onto the first fraction.
More specifically, this suspension is applied onto the first fraction of the sterilized composition by simple spreading of the same, or alternatively by means of a spray dispenser to obtain the colonized composition.
At this point, the process continues comprising a mixing step of the colonized composition in a percentage by weight comprised between 0.1% and 4% with the second fraction of the sterilized composition to obtain a final composition.
In other words, the mixing step consists in joining together a percentage by weight substantially equal to 1% of the colonized composition with a predefined quantity of the sterilized composition, in detail the second fraction.
This means that the colonized composition combined with the sterili ed composition, in this case the second fraction, determines the colonization of the latter too, creating a photo-protective film evenly distributed on the final composition.
Conveniently, the process comprises a second incubation step of the final composition for an operating time substantially equal to 5 days and at an operating temperature comprised between 20°C and 30°C.
The operating temperature is preferably comprised between 22°C and 26°C.
It cannot be ruled out that the process comprises an auxiliary drying step adapted to reduce the moisture content present in the final composition.
In this regard, in accordance with an alternative embodiment, when the process according to the invention is performed with the exclusion of the drying step, it cannot be ruled out from the scope of the present invention that the process itself comprises the auxiliary drying step.
In addition, the presence of microorganisms applied on the surface of the sterilized composition creates a film with thermo-protective action ensuring that the chemical-physical conditions of reproduction of the microorganisms themselves remain unchanged over time. In fact, the fact of providing the basic composition consisting of sterili ed zeolite, eliminates the phenomena of microbial competition between the autochthonous microorganisms physiologically present in the zeolite and the mixture of microorganisms applied.
It cannot be ruled out that the process comprises a fragmentation step of the final composition to obtain a predefined particle size depending on the use of the latter, i.e. of the vegetal surface, or of the type of soil on which it is applied. It is specified that in the present treatise the expression“fragmentation step” means all the operations aimed at determining a dimensional reduction of the final composition, i.e. aimed at reducing the latter to a minimum until it reaches parts of the order of micrometric size.
This means that the expression “fragmentation step” also refers to the micronization of the final composition.
Advantageously, the particle size of the final composition is less than 10 mm. Preferably, the particle size of the final composition is comprised between 3 and 6 mm.
The above-mentioned particle sizes are used for the application of the composition on soils, potted plants, in open fields, or vegetable plants such as e.g. hazel, olive, vines.
At the same time, according to an alternative embodiment, the particle size of the final composition is less than 20 pm.
Preferably, the particle size of the final composition is less than 10 pm.
Specifically, the final composition having the aforementioned particle size is used on vegetal surfaces of the type of leaves, seeds, or roots.
The present invention also relates to the stimulating composition obtained from the previously described process.
Furthermore, the use of the stimulating composition on a vegetal surface cannot be ruled out from the scope of the present discussion.
It is specified that in this discussion the expression“vegetal surface” relates to plants, seeds or growth substrates without distinction.
At the same time, the term“soil” relates to the surface layer covering the earth's crust. It has in practice been ascertained that the described invention achieves the intended objects.
The fact is underlined that the particular solution of applying a mixture of microorganisms onto a sterilized composition comprising zeolites makes it possible to achieve a synergistic effect with the environment surrounding the surface of application of the bio- stimulant.
More specifically, the fact of applying the mixture of microorganisms on the sterilized composition allows creating a protective film against light, temperature variations and the action of bacteria and fungi.
In fact, the crystalline structure of zeolites and zeolitites characterized by cavities and structural channels in their natural state occupied by compensating cations and water molecules allows, in accordance with the present invention, use for inoculation and growth of the mixture of microorganisms.
To this must be added the fact that the crystalline structure of the zeolite allows maintaining the chemical-physical conditions for the growth of the mixture of microorganisms, ensuring their effectiveness over time.
Furthermore, the fact of providing for the fragmentation of the colonized composition increases the surface/volume ratio of the stimulating composition with respect to the surface of application, significantly increasing its effectiveness.

Claims

1) Process for the preparation of a stimulating composition for vegetal surfaces and soils, characterized by the fact that it comprises the steps of:
supplying at least one basic composition comprising zeolite;
inoculating at least one mixture of microorganisms with a first fraction of said basic composition to obtain a colonized composition.
2) Process according to claim 1, characterized by the fact that said mixture of microorganisms comprises at least two microorganisms belonging to the kind chosen from the list comprising: Bacillus, Pseudomonas, Lactobacillus, Streptomyches, Aspergillus, Trichoderma and Cyanobacteria.
3) Process according to one or more of the preceding claims, characterized by the fact that each of said microorganisms has a concentration equal to 102- l08UFC/ml.
4) Process according to one or more of the preceding claims, characterized by the fact that it comprises at least one drying step of said basic composition at a work temperature higher than 80°C to obtain a sterilized composition.
5) Process according to one or more of the preceding claims, characterized by the fact that said work temperature is comprised between 90°C and l70°C.
6) Process according to one or more of the preceding claims, characterized by the fact that it comprises at least one fermentation step of said mixture of microorganisms at an operating temperature higher than 25°C, said fermentation step being prior to said inoculation step.
7) Process according to one or more of the preceding claims, characterized by the fact that it comprises a splitting step of said sterilized composition into said first fraction and into at least a second fraction, said splitting step being subsequent to said drying step.
8) Process according to one or more of the preceding claims, characterized by the fact that it comprises a mixing step of said colonized composition in a percentage by weight comprised between 0.1% and 4% with said second fraction of said sterilized composition to obtain a final composition.
9) Process according to one or more of the preceding claims, characterized by the fact that it comprises a fragmentation step of said final composition to obtain a particle size of less than 10 mm, said fragmentation step being subsequent to said inoculation step.
10) Process according to one or more of the preceding claims, characterized by the fact that said inoculation step comprises a dispersion step of said fermented mixture of microorganisms in an aqueous solvent to obtain a suspension.
11) Process according to one or more of the preceding claims, characterized by the fact that it comprises an application step of said suspension onto said first fraction, said application step being subsequent to said dispersion step.
12) Process according to one or more of the preceding claims, characterized by the fact that said basic composition comprises kaolin.
13) Stimulating composition obtainable from the process according to one or more of claims from 1 to 12.
14) Use of the composition according to claim 13, onto at least one vegetal surface or a soil.
15) Use of the composition according to claim 14, having a particle size of less than 10 mm on soils, plants in the open field and potted plants.
16) Use of the composition according to claim 14, having a particle size of less than 20 pm on vegetal surfaces comprising at least one of leaves, roots, and seeds.
PCT/IB2019/051297 2018-02-19 2019-02-18 Process for the preparation of a stimulating composition for vegetal surfaces and soils WO2019159148A1 (en)

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Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0223662A1 (en) * 1985-10-17 1987-05-27 Fertil France Diffusion Process for making a stable powder of microorganisms and a bacterial inoculum
WO2009027544A1 (en) * 2007-08-27 2009-03-05 Probelte, S.A. Novel biological fertiliser, method for obtaining same and use thereof as a plant growth stimulator

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0223662A1 (en) * 1985-10-17 1987-05-27 Fertil France Diffusion Process for making a stable powder of microorganisms and a bacterial inoculum
WO2009027544A1 (en) * 2007-08-27 2009-03-05 Probelte, S.A. Novel biological fertiliser, method for obtaining same and use thereof as a plant growth stimulator

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
IN HWA KIM ET AL: "Development of a Microbe-Zeolite Carrier for the Effective Elimination of Heavy Metals from Seawater", JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY., vol. 25, no. 9, 28 September 2015 (2015-09-28), KR, pages 1542 - 1546, XP055484544, ISSN: 1017-7825, DOI: 10.4014/jmb.1504.04067 *
JASNA HRENOVIC ET AL: "ZEOLITIZED TUFF AS A CARRIER OF BACTERIA", PROCEEDINGS OF THE 2. SLOVENIAN-CROATIAN SYMPOSIUM ON ZEOLITES / BRONIC, JOSIP ; NOVAK TUSAR, NATASA (UR.). - ZAGREB : CROATIAN ZEOLITE ASSOCIATION , 2009. 71-74, 2 October 2009 (2009-10-02), pages 1 - 4, XP055484534, ISBN: 978-953-55-3731-1, Retrieved from the Internet <URL:https://bib.irb.hr/datoteka/429159.Hrenovic_et_al.pdf> [retrieved on 20180614] *

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