CN109438079A - A kind of solubility bio-organic fertilizer and its production method - Google Patents

A kind of solubility bio-organic fertilizer and its production method Download PDF

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Publication number
CN109438079A
CN109438079A CN201811568546.1A CN201811568546A CN109438079A CN 109438079 A CN109438079 A CN 109438079A CN 201811568546 A CN201811568546 A CN 201811568546A CN 109438079 A CN109438079 A CN 109438079A
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parts
soluble
organic
organic fertilizer
bio
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CN109438079B (en
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丁方军
宋以玲
于建
陈士更
马学文
苏本玉
李巧玉
杨诚
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Shandong Nongda Feiye Technology Co ltd
Shandong Nongda Humic Acid High Efficiency Utilization Engineering Technology Research And Development Co ltd
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Shandong Nongda Humic Acid Efficient Utilization Engineering Technology Research And Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a kind of soluble bio-organic fertilizer and its production methods, the solubility bio-organic fertilizer includes: at least one beneficial microbe, it is acted on by the beneficial microbe application to discharge the SOLID ORGANIC nutrient source of soluble organic nutrient substance, and soluble absorption carrier.The ability that soluble bio-organic fertilizer of the invention has improvement soil, promotes plant growth, improves plant resistance to environment stress and yield, and the solubility bio-organic fertilizer has both easy to produce and is easily applied to integrated excellent results.

Description

A kind of solubility bio-organic fertilizer and its production method
Technical field
The present invention relates to bio-organic fertilizers and water-soluble fertilizer production field, and in particular to a kind of solubility biological organic fertilizer Material and its production method.
Background technique
The rhizosphere bacteria (PGPR) for promoting plant growth is a kind of beneficial root colonization bacterium, and plant can be promoted raw Long, mitigation Plant disease damage.The biological disease of plant growth, realization can be promoted by being inoculated with seed or plant body with beneficial microbe Prevention and treatment, root come from bacterium metabolite such as: amino acid, plant growth regulator, antibiotic substance, or by changing plant Rhizosphere environment and class of antibiotic enhance disease resistance of plant.In addition, some PGPR can also pass through induction disease resistance Mode changes the physiological function of host plant.
Early stage, PGPR was mainly used as biocontrol agent, using less in terms of promoting plant growth.In recent years, with the whole world To reducing fertilizer loss and mitigating fertilizer to the growing interest of surface water, underground water pollution, people promote plant growth to PGPR Research produce new interest again.New target is to promote the PGPR of plant root growth to improve plant to fertilizer by screening The absorption and utilization rate of material, to reduce rate of fertilizer.This method for increasing nutrient uptake and utilization using PGPR is referred to as Integrated management is the joint development target of current many countries.
Fertilizer containing PGPR is successfully used for promoting plant growth, enhancing nutrient absorption and controls biological disease.Example Such as, certain biological products have been demonstrated that plant growth can be promoted, and reduce absorption of the plant to salt under condition of salt stress, reduce line Worm is parasitic, prevents and treats the morbidity of wilt disease, final to improve nutrient uptake and utilization rate, improves yield.
Biological organic fertilizer is primarily referred to as microorganisms with specific functions and mainly with plant and animal residues (such as feces of livestock and poultry, crops Stalk etc. |) it is source and one kind for being combined through harmless treatment, decomposed organic materials has both microbial manure and organic The fertilizer that fertilizer efficiency is answered.Biological organic fertilizer majority used in existing market is powdery and graininess, is primarily adapted for use in ditch spread and cave is applied Fertilizer application mode, and when using solid biologic organic fertilizer, nutrient source in fertilizer must after microorganism is decomposed for a long time ability It is absorbed by plants, utilizes, therefore there are slows, long-acting feature for biological organic fertilizer fertilizer efficiency.With the propulsion of water-fertilizer integral, still Quick-acting dissolved fertilizers are needed, the mode other than equigranular application can be applied by ditch spread, cave and is applied, such as by being sprayed, The application for realizing fertilizer the methods of is applied in drip irrigation and spray irrigation, punching.Presently, there are soluble organic solid Water soluble fertilizer and there is liquid compound micro- Bio-feritlizer, soluble solids organic fertilizer mainly contain the nutriments such as machine potassium, organic matter, other nutrient contents are few, do not contain Beneficial viable bacteria;Liquid composite microorganism fertilizer there are the characteristics that it is volatile, oxidizable, be easy to cause fertilizer efficiency to reduce, thus be considered It is relatively unstable.In addition, liquid fertilizer transportation cost is higher compared with the dissolved fertilizer of concentration.
Summary of the invention
For the above-mentioned prior art, the object of the present invention is to provide a kind of soluble bio-organic fertilizer and its producers Method.Soluble bio-organic fertilizer of the invention has the characteristics that complete water-soluble, easy dispersion, quick-acting, can be adapted for trickle irrigation, punching It the fertilization modes such as applies, spray, being advantageously implemented water-fertilizer integral;And gemma is rich in soluble biological organic fertilizer of the invention The beneficial bacteriums such as Bacillus, trichoderma and azotobacter and soluble saccharide absorb carrier and vitamin, carotenoid and ammonia The additives such as base acid can improve soil, promote plant growth, improve plant resistance to environment stress and yield.
To achieve the above object, the present invention adopts the following technical scheme:
The first aspect of the present invention provides a kind of soluble bio-organic fertilizer, it includes: it is at least one beneficial to micro- life Object is acted on by the beneficial microbe application to discharge the SOLID ORGANIC nutrient source of soluble organic nutrient substance and solvable Property absorb carrier.
Preferably, the beneficial microbe is selected from one of bacillus, azotobacter and trichoderma or a variety of.
Specifically, the beneficial microbe is selected from bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, consolidates One of nitrogen bacterium, Trichoderma viride and Trichoderma harzianum are a variety of.
Preferably, the SOLID ORGANIC nutrient source selects the substances such as corn gluten, soybean protein, potassium fulvate and fermentation molasses One of or it is a variety of.
Preferably, the soluble carrier that absorbs is selected from one of glucose, sucrose and fructose or a variety of.
Further, also contain additive in the soluble organic fertilizer, the additive be amino acid, vitamin and One of carotenoid is a variety of.
Preferably, the soluble bio-organic fertilizer, it includes:
20-45 parts of SOLID ORGANIC nutrient source, 12-25 parts of beneficial microbe, soluble absorption carrier 10-20 parts, additive 0-0.5 parts.
It is furthermore preferred that the solubility bio-organic fertilizer is made of the material combination any in following (1)-(4):
(1) corn gluten 15-25 parts, 5-15 parts of soybean protein, 4-6 parts of potassium fulvate, 12-16 parts of beneficial microbe, can Dissolubility absorbs carrier 10-20 parts, 0.1-0.5 parts of additive;
(2) potassium fulvate 5-15 parts, fermentation molasses 4-6 parts, 15-25 parts of soybean protein, 15-25 parts of beneficial microbe, can Dissolubility absorbs carrier 10-20 parts, 0.1-0.5 parts of additive;
(3) corn gluten 15-25 parts, 5-15 parts of soybean protein, 5-15 parts of potassium fulvate, 12-16 parts of beneficial microbe, can Dissolubility absorbs carrier 10-20 parts, 0.1-0.5 parts of additive;
(4) soybean protein 5-15 parts, 5-15 parts of potassium fulvate, 12-16 parts of beneficial microbe, soluble absorption carrier 10- 20 parts.
The second aspect of the present invention provides the production method of above-mentioned soluble bio-organic fertilizer, comprising the following steps:
SOLID ORGANIC nutrient source is cultivated together at least one beneficial microbe, obtains liquefaction organic nutrition source;From liquid Change and extract soluble organic nutrient substance in organic nutrition source, and soluble organic nutrient substance is adsorbed onto soluble absorb and is carried On body.
Preferably, breeding condition are as follows: 35-40 DEG C, aerobic fermentation 10-20 days.
Preferably, soluble organic nutrient substance is extracted from fluidized solid organic nutrition source by the method for filtering.
Preferably, by mixing, spraying and dry method soluble organic nutrient substance is adsorbed onto soluble absorption On carrier.
Beneficial effects of the present invention:
(1) soluble bio-organic fertilizer of the invention have improvement soil, promote plant growth, improve plant resistance to environment stress and The ability of yield, and the solubility organic fertilizer has both easy to produce and is easily applied to integrated excellent results.
(2) compared with common biological organic fertilizer, soluble biological organic fertilizer of the invention has full water-soluble, easily divides Dissipate, fertilizer efficiency is fast, nutrient formula is easy to the advantages that adjusting, it is slow to solve the problems, such as that existing biological organic fertilizer works.
(3) compared with common water soluble organic fertilizer, soluble biological organic fertilizer nutrient content of the invention is high, rich in having Beneficial viable bacteria can improve rhizosphere soil environment and enhancing plant resistance to environment stress, promote plant growth and the absorption to nutriment.
Specific embodiment
Following detailed description is all exemplary, it is intended to provide further instruction to the application.Unless otherwise indicated, herein All technical and scientific terms used have and the normally understood meaning phase of the application technical field those of ordinary skill Together.
The present invention provides a kind of soluble biological organic fertilizers, which can be dissolved in water and/or liquid is organic In fertilizer.In some embodiments of the present invention, the soluble bio-organic fertilizer is solid or gel form.
SOLID ORGANIC nutrient source refers to the substance containing organic nutrient, organic nutrition source may be from part animal and plant body, The mineral of animal byproduct and exploitation.In certain embodiments, the organic nutrient substance in SOLID ORGANIC nutrient source is insoluble.
Term " liquefaction " means to decompose, and in certain embodiments, SOLID ORGANIC nutrient source needs are liquefied.Certain In embodiment, SOLID ORGANIC nutrient source is by Microorganism Liquefaction.SOLID ORGANIC nutrient source can also be by known in the art Other methods liquefaction.
" soluble organic nutrient substance " refers to the organic nutrient substance of dissolution.In certain embodiments, soluble Organic nutrient substance can be dissolved in water and/or liquid organic fertilizer.
Soluble organic fertilizer can nitrogenous (N), phosphorus (P) and potassium (K) a variety of macronutrients, the NPK value of fertilizer is fertilizer The ratio of nitrogen, phosphorus and potassium in material.There may also be measure nutrient and boron, manganese, zinc, copper, iron, molybdenum in such as calcium, magnesium and sulphur etc. Equal microelements.
In certain embodiments, soluble biological organic fertilizer includes one or more beneficial microbes.
Term " microorganism " includes microorganism and microorganism consortium.Beneficial microbe is to be applied to seed, soil Or plant growth can be promoted when plant and/or improve utilization of nutrients.In certain embodiments, beneficial microbe makes Organic nutrient substance mineralising in fertilizer, thus improve be applied to seed, in soil or plant fertilizer utilization rate.Beneficial to micro- Biology can also be by inside and outside control seed, inside and outside plant and one of soil or multiple pathogens improve plant resistance to environment stress. In certain embodiments, beneficial microorganism also has the function of improving soil quality.Beneficial microbe include but It is not limited to bacillus, fusobacterium, Rhodopseudomonas, rhizobium, azotobacter, pseudomonas, Arthrobacter, Huang The floras such as Bacillus, saccharomyces, streptomyces, Penicillium and trichoderma.All mentioned microorganisms all can be from CICC resources bank It obtains.In certain embodiments, the beneficial microbe in soluble bio-organic fertilizer is with every gram or every milliliter about 1 × 102To 1 ×1015A bacterium colony body exists.In the present invention, beneficial microbe can control inside and outside seed, inside and outside plant or in soil The amount of one or more pathogen.
Therefore, soluble biological organic fertilizer of the invention is in addition to containing nutritional ingredient possessed by soluble organic fertilizer, also Contain one in bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, nitrogen-fixing bacteria, Trichoderma viride and Trichoderma harzianum Kind or multiple beneficial microorganism.
In certain embodiments, carrier also is absorbed containing soluble in soluble biological organic fertilizer of the invention.It is described Solubility absorbs carrier and is selected from one of glucose, sucrose and fructose or a variety of.On the one hand it is micro- life that solubility, which absorbs carrier, Nutrition needed for object provides;On the other hand it needs through the soluble concentration class for absorbing carrier and improving soluble nutrient and microorganism, Reduce the loss late of spray-drying process microorganism.
In order to further enhance the function of soluble biological organic fertilizer of the invention, in the solubility biological organic fertilizer Also contain the components such as vitamin, carotenoid and amino acid as additive.
The step of preparing soluble biological organic fertilizer includes that soluble organic nutrient substance is extracted from SOLID ORGANIC nutrient source Matter.SOLID ORGANIC nutrient source usually contains main and secondary nutriment.
Soluble organic nutrient substance is extracted from SOLID ORGANIC nutrient source, comprising: by SOLID ORGANIC nutrient source and at least A kind of beneficial microbe is cultivated together, obtains liquefaction organic nutrition source;Soluble organic nutrition is extracted from liquefaction organic nutrition source Substance, and soluble organic nutrient substance is made to be adsorbed onto soluble absorb on carrier.
Wherein, beneficial microbe can come from bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, fixed nitrogen The floras such as bacterium, Trichoderma viride, Trichoderma harzianum.
It include being removed not from liquefaction organic nutrition source from soluble organic nutrient substance is extracted in liquefaction organic nutrition source Molten substance, insoluble matter mainly include threadiness residual organic materials, can by adjust pH, filtering or it is known in the art its Its method will be precipitated to be removed from liquefaction organic nutrition source.
Remove obtained soluble organic nutrient substance after insoluble substance can be immersed in it is soluble absorb in carrier or On carrier.In certain embodiments, by by soluble organic nutrient substance with solubility absorb carrier mix, it is spraying and dry Dry method obtains soluble biological organic fertilizer.The typical soluble carrier that absorbs includes glucose, sucrose and fructose, certain In embodiment, drying is completed by reducing heat levels.
In certain embodiments, the soluble bio-organic fertilizer of different formulations is prepared to meet seed, soil or plant The particular demands of object.In certain case study on implementation, the soluble bio-organic fertilizer for applying a certain special formulation can promote to plant Strain growth, the absorption for improving Plant To Nutrient substance, and reduce one or more in inside and outside seed, plant inside and outside or soil Pathogen there are quantity.
The invention also includes enhancing plant growth when soluble biological organic fertilizer is applied to seed, soil or plant and/ Or the method for crop yield, such as: fertilizer is applied to by plant by the method for foliage-spray, can also be applied, be planted by trickle irrigation, punching Fertile and spraying form is applied on seed, soil and plant, and can be applied in seed, soil in the different stages of growth of plant On earth and plant.
It is a kind of to be liquefied comprising a large amount of and Meso- and micro-nutrients SOLID ORGANIC nutrient sources, this SOLID ORGANIC nutrient source Mainly include: corn gluten, soybean protein, potassium fulvate and fermentation molasses.SOLID ORGANIC nutrient source is ground into very first Small particle, then these molecules with bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, nitrogen-fixing bacteria, One or more beneficial microbe colonies such as Trichoderma viride, Trichoderma harzianum are inoculated with.By connecing containing powder particle and microorganism Kind object solution aerobic fermentation about 15 days, then filters solution.Soluble absorption carrier is added into filtrate (such as: glucose, sugarcane Sugar and fructose), then soluble bio-organic fertilizer is made by the method for cold nebulization, drying.
To sum up, soluble biological organic fertilizer of the invention ferments to SOLID ORGANIC nutrient source using beneficial microbe, Nutrient quick-actingization in organic materials;And the beneficial microbe being added is existed in the form of viable bacteria, and soil can be improved Earth rhizospheric environment and enhancing plant resistance to environment stress, promote plant growth and the absorption to nutriment.
SOLID ORGANIC nutrient source used in the present invention is mainly corn gluten, soybean protein, potassium fulvate and sugar fermentation Honey, it is from a wealth of sources, be rich in macronutrient, middle amount nutrient and micronutrient element, and nutrient formula be easy into Row adjustment.Beneficial microbe used in the present invention is selected from one of bacillus, azotobacter and trichoderma or a variety of, Without antagonism between above-mentioned beneficial microbe, be used in conjunction with and also help fermentation, further improve rhizosphere soil environment and Enhance plant resistance to environment stress, promotes plant growth and the absorption to nutriment.The selection of beneficial microbe type is very crucial, this hair It is bright that multiple-microorganism is selected during the test, for example, by using bacillus subtilis, Pediococcus pentosaceus, aspergillus niger, The composite flora that bacillusmusilaginosiengineering, Rhizopus oryzae, pichia farinose, Sphingobacterium multivorum and ferment bacterium form, as a result It was found that it is inferior to beneficial microbe used in the present invention to the ferment effect of SOLID ORGANIC nutrient source.
Currently marketed biological organic fertilizer is mainly powdery and column, is mainly used for ditch spread, cave is applied based on feces of livestock and poultry Want biological organic fertilizer made of raw material.And its good water solubility of soluble biological organic fertilizer prepared by the present invention, good dispersion can fit It for trickle irrigation, rushes and the fertilization modes such as applies, sprays, be advantageously implemented water-fertilizer integral.
The water-soluble biological organic fertilizer of existing report is that selection is water-soluble to improve the method that the water solubility of fertilizer mainly uses Property good organic material, be then inoculated with strain and carry out compost fermentation, then the material after fermentation is ground.Although in this way can be with The water solubility of biological organic fertilizer is improved to a certain extent, but on the one hand, the type selection of organic material is limited;Another party Face, organic fertilizer mainly kill original pest, weed seed in material, hippuric acid, allantoic acid etc. during compost maturity Hot fermentation is completed in the decomposition of noxious material, is removed foul smell, while adjusting the carbon-nitrogen ratio of organic materials, is formed by organic Fertilizer mainly contains machine matter, also has slow, thus compost containing nutriments, these nutrients itself such as the organic N P and Ks in part It is not after utilizing microbial fermentation nutrient quick-actingization in organic materials, therefore crop also needs micro- life when being absorbed and utilized The decomposition and inversion of object and enzyme is finally absorbed by crops utilization.Unlike existing water-soluble biological organic fertilizer, to solve life The water-soluble problem of organic fertilizer, the present invention promote a large amount of in organic materials using the aerobic metabolism fermentation by microorganism Decomposition with middle microelement discharges, and forms small soluble molecules substance, and has and filter this important step, ensure that last The dissolubility of gained dissolved fertilizer, and the nutriment in present invention gained fertilizer all derives from Institute of Micro-biology's decomposition Natural material does not add any other synthetic, and crop is not necessarily to further decomposition and inversion when being absorbed and utilized, can be fast Effect absorbs, and it is slow to solve the problems, such as that the water-soluble biological organic fertilizer of existing report works.More it is essential that with existing water-soluble Property biological organic fertilizer is compared, and beneficial microorganism additional amount is significantly increased in soluble biological organic fertilizer of the invention, these Beneficial microbe group is captured by nutrient competition, space, and it is antibacterial to generate antibiotic, bacteriocin, thermophilic iron element, extracellular lytic enzyme etc. Substance can form protective barrier around crop root, with bacterium gram bacterium, inhibit harmful fungoid, the growth of bacterium, breeding, Yi Jizhi Object pathogen is infected, purification soil environment, mitigates plant disease incidence to the maximum extent.Bacterial population and actinomyces in soil Several increases is conducive to Soil Nutrient Transformation again, and then improves soil nutrient availability, makes between soil nutrient and edaphon Good win-win progress relationship is formed, provides an excellent soil environment for plant growth.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention is the test material of this field routine, can pass through commercial channel It is commercially available.
Embodiment 1: the preparation of soluble biological organic fertilizer
1. raw material forms:
20 parts of corn gluten, 10 parts of soybean protein, 5 parts of potassium fulvate, 10 parts of bacillus, nitrogen-fixing bacteria (CICC 20603) 5 parts, 5 parts of Trichoderma harzianum, soluble absorption 10 parts of carrier, 0.3 part of additive, 40 parts of water;
Wherein, the bacillus includes 5 parts of bacillus subtilises (CICC 10732), 5 parts of bacillus amyloliquefaciens (CICC 10036);The soluble carrier that absorbs includes 5 parts of glucose and 5 parts of fructose;The additive includes 0.1 part of amino Sour 0.2 part of vitamin C.
2. preparation method:
First corn gluten, soybean protein, potassium fulvate mixing are ground and be sufficiently mixed with water, the good gemma of inoculated and cultured Bacillus, nitrogen-fixing bacteria and Trichoderma harzianum, add additive, are made into liquid product mix.This liquid product mix is at 37 DEG C Left and right carries out aerobic fermentation about 15 days, then by filtering fermentation liquor, then soluble absorption carrier, last low temperature is added into filtrate It is spraying, dry that soluble bio-organic fertilizer is made.Water content≤5% of gained solubility bio-organic fertilizer.
Embodiment 2: the preparation of soluble biological organic fertilizer
1. raw material forms:
10 parts of potassium fulvate, 5 parts of molasses of fermentation, 20 parts of soybean protein, 10 parts of bacillus, 10 parts of Trichoderma, solubility Absorb 15 parts of carrier, 0.4 part of additive, 30 parts of water;
The bacillus includes 5 parts of bacillus amyloliquefaciens (CICC 10036) and 5 parts of bacillus licheniformis;The wood Mould includes 5 parts of Trichoderma virides (CICC 13029) and 5 parts of Trichoderma harzianums (CICC 41290);The soluble absorption carrier packet Include 10 glucose, 5 portions of sucrose;The additive includes 0.2 part of amino acid and 0.2 part of carotenoid.
2. preparation method:
First potassium fulvate, soybean protein mixing are ground and be sufficiently mixed with fermentation molasses and water, the good bud of inoculated and cultured Spore bacillus and Trichoderma are added additive, are made into liquid product mix.This liquid product mix is carried out at 37 DEG C or so Aerobe fermentation about 15 days, then by filtering fermentation liquor, then be added into filtrate it is soluble absorb carrier cold nebulization, dry be made can Dissolubility bio-organic fertilizer.Water content≤5% of gained solubility bio-organic fertilizer.
Embodiment 3: the preparation of soluble biological organic fertilizer
1. raw material forms:
20 parts of corn gluten, 10 parts of soybean protein, 10 parts of potassium fulvate, 5 parts of bacillus, 5 parts of Trichoderma, nitrogen-fixing bacteria 5 Part, soluble absorption 15 parts of carrier, 0.5 part of additive, 40 parts of water;
The bacillus is bacillus subtilis (CICC 10732), and the Trichoderma is Trichoderma viride (CICC 13029);The soluble carrier that absorbs includes 10 glucose, 5 portions of sucrose;The additive includes 0.2 part of vitamin C, 0.2 Part amino acid and 0.1 part of carotenoid.
2. preparation method:
First by corn gluten, soybean protein, potassium fulvate, mixing grinds and is sufficiently mixed with water, the good bud of inoculated and cultured Spore bacillus, Trichoderma, are made into liquid product mix.This liquid product mix is in 37 DEG C or so progress aerobic fermentations about 15 It, then by filtering fermentation liquor, then soluble absorption carrier and additive is added into filtrate, last cold nebulization, dry system Obtain soluble bio-organic fertilizer.Water content≤5% of gained solubility bio-organic fertilizer.
Embodiment 4: the preparation of soluble biological organic fertilizer
1. raw material forms:
10 parts of soybean protein, 10 parts of potassium fulvate, 15 parts of bacillus, soluble absorption 15 parts of carrier, 40 parts of water;
The bacillus includes 10 parts of bacillus subtilises (CICC 10732) and 5 parts of bacillus amyloliquefaciens (CICC 10036);The soluble carrier that absorbs includes 10 parts of glucose and 5 portions of sucrose.
2. preparation method:
First soybean protein, potassium fulvate mixing are ground and are sufficiently mixed with water, the good bacillus of inoculated and cultured is made into Liquid product mix.This mixture 37 DEG C or so progress aerobic fermentation about 15 days, then by filtering fermentation liquor, then to filtrate 10-20 parts of middle addition soluble absorption carriers, last cold nebulization, dry obtained soluble bio-organic fertilizer.Gained is solvable Property bio-organic fertilizer water content≤5%.
Test example 1: dissolubility measurement
The soluble organic fertilizer solubility property in water of embodiment 1-4 preparation is measured, is prepared by embodiment 1-4 Soluble biological organic fertilizer dissolved respectively with the water of different multiples, when record fertilizer is completely dissolved required in water Between, it the results are shown in Table 1.
Table 1: the time required to fertilizer prepared by different embodiments is completely dissolved in water
Test example 2: fertilizer nutrient assay
The nutrient content of the soluble organic fertilizer of embodiment 1-4 preparation is measured, the results are shown in Table 2.
Table 2: fertilizer nutrient content table prepared by each embodiment (in terms of butt)
Test example 3: the application in radish planting
In order to verify the fertilizer efficiency of bio-organic fertilizer of the invention soluble, using can made from the embodiment of the present invention 1,2,3 Dissolubility bio-organic fertilizer is tested in radish planting field.
1. test material and method
1.1 test periods and place
Test site: Tai'an Shandong NongDa Fertilizer Science Co., Ltd radish cultivates base, and kind is fruit sand nest trailing plants Foretell.
Test period: in September, 2017-November.
1.2 experimental design
Test sets 5 processing, handles 1 blank, the 2 commercially available common organic water-soluble fertilizer of application of processing, 3 application embodiment of processing Solubility biological organic fertilizer made from embodiment 2 is applied in solubility biological organic fertilizer made from 1, processing 4, and 5 application of processing is implemented Solubility biological organic fertilizer made from example 3,3 repetitions of each processing, the total dosage 120kg/667m of the fertilizer of every group of processing2, general Logical organic fertilizer whole base is applied, and soluble organic fertilizer base applies 50kg/667m2, a fertilizer is imposed in conjunction with watering within every 15 days after field planting, Each dosage 10kg/667m2, continuous top dressing 5 times, every group of experimental plot area 67m2
2. experimental result
Influence of the soluble bio-organic fertilizer of 2.1 present invention to radish growth and yield
Crop leaf area, Weight per plant, dry matter content and biological yield can reflect to a certain degree plant growth and development and Yield situation, as can be seen from Table 3: present invention solubility biological organic fertilizer 1,2,3 is promoting radish growth, is improving radish yield Aspect has remarkable result, and compared with common commercially available organic water-soluble fertilizer, leaf area has been respectively increased 24.72%, 28.04%, 32.06%, biological yield has been respectively increased 2.69%, 4.67%, 6.58%, has apparent growth-promoting and volume increase effect It answers.
Influence of 3. different disposal of table to radish growth and yield
Influence of the soluble bio-organic fertilizer of 2.2 present invention to radish quality
As shown in Table 4, it applies commercially available organic water-soluble fertilizer and improves VC in radish, soluble protein and soluble sugar content, Compared with processing 2, applies the VC content that soluble biological organic fertilizer 1,2,3 is handled and has been respectively increased 4.93%, 6.34%, 4.23%, soluble protein content has been respectively increased 10.65%, 13.02%, 23.08%, and soluble sugar content is respectively increased 5.65%, 3.22%, 11.78%, therefore soluble bio-organic fertilizer has very big development latent in terms of improving crop quality Power.
Influence of 4. different disposal of table to radish quality
Influence of the soluble bio-organic fertilizer of 2.3 present invention to soil microbe quantity
Soil microbial biomass directly affects soil biologic characteristic, soil nutrient composition and conversion and stable soil granule The function of structure.As shown in Table 5, it compared with processing 1, applies commercially available organic water-soluble fertilizer and improves rhizosphere soil bacterium and actinomyces Number reduces rhizosphere soil fungi number, apply soluble bio-organic fertilizer 1,2,3 further improve rhizosphere soil bacterium and Quantity of actinomyces, while fungi number is reduced, wherein 24.92% and 49.43% has been respectively increased in bacterial population and quantity of actinomyces, 28.43% and 65.40% and 35.78% and 63.12%, and fungi number reduces 3.05%, 5.08%, 12.40%, most " fungi type " soil is converted to " bacteria type " soil at last.These beneficial microbe groups are captured by nutrient competition, space, are produced The antibacterial substances such as raw antibiotic, bacteriocin, thermophilic iron element, extracellular lytic enzyme can form protective barrier around crop root, with bacterium Gram bacterium inhibits infecting for harmful fungoid, the growth of bacterium, breeding and phytopathogen, purification soil environment, to greatest extent Ground mitigates plant disease incidence.The increase of bacterial population and quantity of actinomyces is conducive to Soil Nutrient Transformation, Jin Erti again in soil High soil nutrient availability makes to form good win-win progress relationship between soil nutrient and edaphon, mention for plant growth For an excellent soil environment.
Influence of 5. different disposal of table to rhizosphere soil microorganism quantity
Influence of the soluble bio-organic fertilizer of 2.4 present invention to soil nutrient
Influence for the soluble bio-organic fertilizer of the verifying present invention to Content of available nutrient in soils, plantation took soil after 90 days Nutrient content in soil is surveyed, as shown in Table 6, applies the soil available nitrogen, effectively of the soluble bio-organic fertilizer 1,2,3 of the present invention Phosphorus, quick-acting potassium content are improved largely compared with applying commercially available soluble organic fertilizer, it has been respectively increased 5.47%~ 10.84%, 11.48%~11.97%, 13.54%~17.46%.Soil organic matter content is improved simultaneously, thus is improved The water conservation of soil, nutrient preserving capability promote absorption and utilization of the plant to nutriment in soil, reduce leaching loss of nutrient, improve Utilization rate of fertilizer.
Influence of 6. different disposal of table to the readily available nutrient of soil and the content of organic matter
Influence of the soluble biological organic fertilizer of 2.5 present invention to soil enzyme activities
Soil enzyme is to decompose the one kind being released in soil by edaphon, the secretion of animals and plants living body and animals and plants remnant Bioactive substance with catalytic capability, therefore its activity is closely related with the metabolic activity of microorganism.As shown in Table 7, with Commercially available organic water-soluble fertilizer is compared, and applying soluble bio-organic fertilizer 1,2,3 reduces soil urease liveness, hence it is evident that improves Soil fixed NH_4+, Soil neutral phosphatase and sucrase active, thus the leaching loss of soil available nitrogen element is reduced, it accelerates organic The release of the Threshing injury and soil phosphorus of phosphorus, improves soil rotten degree, accelerates organotrophic decomposition and release, improves Nutrient availability;Optimize the quantity and structure of soil microbial community, improves absorption and utilization, increasing of the crop to nutriment Strong crop resistance.
Influence of 7. different disposal of table to soil enzyme activities
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field For art personnel, various changes and changes are possible in this application.It is all within the application scope and principle, done any repair Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.

Claims (10)

1. a kind of solubility bio-organic fertilizer, which is characterized in that it includes: at least one beneficial microbe, by described beneficial Microorganism application is acted on to discharge the SOLID ORGANIC nutrient source of soluble organic nutrient substance, and soluble absorption carrier.
2. solubility bio-organic fertilizer according to claim 1, which is characterized in that the beneficial microbe is selected from gemma One of Bacillus, azotobacter and trichoderma are a variety of.
3. solubility bio-organic fertilizer according to claim 1, which is characterized in that the SOLID ORGANIC nutrient source is selected from One of corn gluten, soybean protein, potassium fulvate and fermentation molasses are a variety of.
4. solubility bio-organic fertilizer according to claim 1, which is characterized in that the soluble carrier that absorbs is selected from One of glucose, sucrose and fructose are a variety of.
5. solubility bio-organic fertilizer according to claim 1, which is characterized in that the solubility bio-organic fertilizer In also contain additive;Preferably, the additive is one of amino acid, vitamin C and carotenoid or a variety of.
6. solubility bio-organic fertilizer according to claim 1-5, which is characterized in that the soluble biology Organic fertilizer, it includes:
20-45 parts of SOLID ORGANIC nutrient source, 12-25 parts of beneficial microbe, soluble absorption carrier 10-20 parts, additive 0-0.5 Part;
Preferably, the soluble bio-organic fertilizer is made of the material combination any in following (1)-(4):
(1) corn gluten 15-25 parts, 5-15 parts of soybean protein, 4-6 parts of potassium fulvate, 12-16 parts of beneficial microbe, solubility Absorb carrier 10-20 parts, 0.1-0.5 parts of additive;
(2) potassium fulvate 5-15 parts, molasses 4-6 parts of fermentation, 15-25 parts of soybean protein, 15-25 parts of beneficial microbe, solubility Absorb carrier 10-20 parts, 0.1-0.5 parts of additive;
(3) corn gluten 15-25 parts, 5-15 parts of soybean protein, 5-15 parts of potassium fulvate, 12-16 parts of beneficial microbe, solubility Absorb carrier 10-20 parts, 0.1-0.5 parts of additive;
(4) soybean protein 5-15 parts, 5-15 parts of potassium fulvate, 12-16 parts of beneficial microbe, soluble carrier 10-20 parts of absorption.
7. the production method of solubility bio-organic fertilizer described in any one of claims 1-6, which is characterized in that including following Step:
SOLID ORGANIC nutrient source is cultivated together at least one beneficial microbe, obtains liquefaction organic nutrition source;Have from liquefaction Soluble organic nutrient substance is extracted in machine nutrient source, and soluble organic nutrient substance is adsorbed onto soluble absorption carrier On.
8. production method according to claim 7, which is characterized in that breeding condition are as follows: 35-40 DEG C, aerobic fermentation 10-20 It.
9. production method according to claim 7, which is characterized in that by the method for filtering from fluidized solid organic nutrition Soluble organic nutrient substance is extracted in source.
10. production method according to claim 7, which is characterized in that by mixing, spraying and dry method will be solvable Property organic nutrient substance be adsorbed onto soluble absorb on carrier.
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