WO2009017087A1 - 固定化リパーゼおよびその製造方法 - Google Patents

固定化リパーゼおよびその製造方法 Download PDF

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Publication number
WO2009017087A1
WO2009017087A1 PCT/JP2008/063509 JP2008063509W WO2009017087A1 WO 2009017087 A1 WO2009017087 A1 WO 2009017087A1 JP 2008063509 W JP2008063509 W JP 2008063509W WO 2009017087 A1 WO2009017087 A1 WO 2009017087A1
Authority
WO
WIPO (PCT)
Prior art keywords
immobilized lipase
producing
same
lipase
immobilized
Prior art date
Application number
PCT/JP2008/063509
Other languages
English (en)
French (fr)
Inventor
Eiji Iwaoka
Toshiharu Arishima
Original Assignee
Fuji Oil Company, Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Oil Company, Limited filed Critical Fuji Oil Company, Limited
Priority to US12/452,947 priority Critical patent/US20100216209A1/en
Priority to EP08791744.9A priority patent/EP2177608B1/en
Priority to JP2009525392A priority patent/JP5177143B2/ja
Priority to DK08791744.9T priority patent/DK2177608T3/en
Publication of WO2009017087A1 publication Critical patent/WO2009017087A1/ja

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

 従来よりエステル交換活性が向上した、固定化リパーゼを提供することを目的とした。リパーゼ活性を有する酵素,高分子乳化剤および油脂を含む水中油型乳化物を担体に固定化することで、エステル交換活性が格段に向上した固定化リパーゼを得ることができた。
PCT/JP2008/063509 2007-07-31 2008-07-28 固定化リパーゼおよびその製造方法 WO2009017087A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/452,947 US20100216209A1 (en) 2007-07-31 2008-07-28 Immobilized lipase and method for producing the same
EP08791744.9A EP2177608B1 (en) 2007-07-31 2008-07-28 Immobilized lipase and method for producing the same
JP2009525392A JP5177143B2 (ja) 2007-07-31 2008-07-28 固定化リパーゼおよびその製造方法
DK08791744.9T DK2177608T3 (en) 2007-07-31 2008-07-28 Immobilized lipase and process for producing the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007199240 2007-07-31
JP2007-199240 2007-07-31

Publications (1)

Publication Number Publication Date
WO2009017087A1 true WO2009017087A1 (ja) 2009-02-05

Family

ID=40304317

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063509 WO2009017087A1 (ja) 2007-07-31 2008-07-28 固定化リパーゼおよびその製造方法

Country Status (6)

Country Link
US (1) US20100216209A1 (ja)
EP (1) EP2177608B1 (ja)
JP (1) JP5177143B2 (ja)
DK (1) DK2177608T3 (ja)
MY (1) MY150945A (ja)
WO (1) WO2009017087A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5585454B2 (ja) * 2009-02-04 2014-09-10 不二製油株式会社 乳化粉末リパーゼ製剤の製造方法
JP2021058180A (ja) * 2019-10-09 2021-04-15 アサヒビール株式会社 容器詰飲料及びその製造方法
WO2022085419A1 (ja) * 2020-10-19 2022-04-28 不二製油グループ本社株式会社 酵素固定化用担体及びそれを用いた固定化酵素

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022003478A (es) * 2019-09-23 2022-04-25 Unilever Ip Holdings B V Emulsion de aceite en agua con proteinas vegetales.

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6098984A (ja) 1983-09-05 1985-06-01 ノボ ノルディスク アクティーゼルスカブ 固定化リパ−ゼ調製品の製造方法
JPS61202688A (ja) 1985-02-27 1986-09-08 ノボ ノルディスク アクティーゼルスカブ 固定化リパーゼ調製品の製造方法
JPS62134090A (ja) 1985-12-07 1987-06-17 Fuji Oil Co Ltd 酵素剤の製造法
JPH02138986A (ja) * 1987-12-22 1990-05-28 Unilever Nv 脂肪酸エステルの製造方法
JPH0361485A (ja) 1989-07-31 1991-03-18 Ajinomoto Co Inc 固定化リパーゼ酵素剤
JPH07265073A (ja) 1994-03-31 1995-10-17 Fuji Oil Co Ltd 高エステル交換活性酵素剤およびその製造方法
JPH11500319A (ja) * 1995-06-27 1999-01-12 ユニリーバー・ナームローゼ・ベンノートシャープ 固定された酵素及びトリグリセリド油の加工への使用
JP2000062795A (ja) 1998-08-14 2000-02-29 Totani Giken Kogyo Kk プラスチック袋およびその製造方法
JP2001506499A (ja) * 1996-12-19 2001-05-22 ユニリーバー・ナームローゼ・ベンノートシャープ 固定化酵素及びトリグリセリド油の処理のためのその使用
JP2009038986A (ja) 2007-08-06 2009-02-26 Kubota Corp 歩行型草刈機

Family Cites Families (14)

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US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
DK73592D0 (da) * 1992-06-03 1992-06-03 Novo Nordisk As Nyt enzym
EP0750848B1 (en) * 1994-12-22 2003-04-23 Kao Corporation Modifier and modifier composition for protein-containing substance
DE69732775T2 (de) * 1996-04-12 2006-01-26 Novozymes A/S Enzymhaltige granulatkörner sowie verfahren zu deren herstellung
JP3589904B2 (ja) * 1999-06-17 2004-11-17 花王株式会社 酸性水中油型乳化組成物
JP3779505B2 (ja) * 1999-08-24 2006-05-31 花王株式会社 油脂組成物
JP4116844B2 (ja) * 2001-09-13 2008-07-09 花王株式会社 油脂組成物
IL152290A0 (en) * 2002-10-14 2003-05-29 Enzymotec Ltd Immobilization of compounds on polymeric matrix
JP4381037B2 (ja) * 2003-06-04 2009-12-09 花王株式会社 油脂組成物
DE10337451A1 (de) * 2003-08-14 2005-03-17 Cognis Deutschland Gmbh & Co. Kg Verwendung von PIT-Emulsionen in enzymatischen Reaktionen
EP1771554A4 (en) * 2003-12-19 2008-03-05 Syngenta Participations Ag MICROBIAL EXPRESSED XYLANASES, THEIR USE AS FOOD ADDITIVES AND OTHER APPLICATIONS
WO2006006521A1 (ja) * 2004-07-13 2006-01-19 Fuji Oil Company, Limited 多糖類と蛋白質との複合体並びにこれを含む乳化剤及び乳化物
DE102005002700A1 (de) * 2005-01-19 2006-07-27 Cognis Deutschland Gmbh & Co. Kg Zusammensetzungen verwendbar als Biotreibstoff
US8067052B2 (en) * 2005-02-25 2011-11-29 Siddharth Shastri Nutritional composition and a container for the convenient transport and storage of the nutritional composition

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6098984A (ja) 1983-09-05 1985-06-01 ノボ ノルディスク アクティーゼルスカブ 固定化リパ−ゼ調製品の製造方法
JPS61202688A (ja) 1985-02-27 1986-09-08 ノボ ノルディスク アクティーゼルスカブ 固定化リパーゼ調製品の製造方法
JPS62134090A (ja) 1985-12-07 1987-06-17 Fuji Oil Co Ltd 酵素剤の製造法
JPH02138986A (ja) * 1987-12-22 1990-05-28 Unilever Nv 脂肪酸エステルの製造方法
JPH0361485A (ja) 1989-07-31 1991-03-18 Ajinomoto Co Inc 固定化リパーゼ酵素剤
JPH07265073A (ja) 1994-03-31 1995-10-17 Fuji Oil Co Ltd 高エステル交換活性酵素剤およびその製造方法
JPH11500319A (ja) * 1995-06-27 1999-01-12 ユニリーバー・ナームローゼ・ベンノートシャープ 固定された酵素及びトリグリセリド油の加工への使用
JP2001506499A (ja) * 1996-12-19 2001-05-22 ユニリーバー・ナームローゼ・ベンノートシャープ 固定化酵素及びトリグリセリド油の処理のためのその使用
JP2000062795A (ja) 1998-08-14 2000-02-29 Totani Giken Kogyo Kk プラスチック袋およびその製造方法
JP2009038986A (ja) 2007-08-06 2009-02-26 Kubota Corp 歩行型草刈機

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BUTHE A. ET AL.: "Generation of lipase-containing static emulsions in silicone spheres for synthesis in organic media", J. MOL. CATAL. B: ENZYMATIC, vol. 35, no. 4-6, 2005, pages 93 - 99, XP005027013 *
LAMB S.B. ET AL.: "Enzyme immobilisation on colloidal liquid aphrons (CLAs): influence of protein properties", ENZYME MICROB. TECHNOL., vol. 24, 1999, pages 541 - 548, XP008129481 *
See also references of EP2177608A4 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5585454B2 (ja) * 2009-02-04 2014-09-10 不二製油株式会社 乳化粉末リパーゼ製剤の製造方法
JP2021058180A (ja) * 2019-10-09 2021-04-15 アサヒビール株式会社 容器詰飲料及びその製造方法
WO2022085419A1 (ja) * 2020-10-19 2022-04-28 不二製油グループ本社株式会社 酵素固定化用担体及びそれを用いた固定化酵素

Also Published As

Publication number Publication date
JPWO2009017087A1 (ja) 2010-10-21
MY150945A (en) 2014-03-14
EP2177608B1 (en) 2015-10-14
DK2177608T3 (en) 2015-12-14
EP2177608A1 (en) 2010-04-21
EP2177608A4 (en) 2010-11-24
JP5177143B2 (ja) 2013-04-03
US20100216209A1 (en) 2010-08-26

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