WO2008136014A1 - Clonage et expression de l'entérokinase et son procédé de production - Google Patents

Clonage et expression de l'entérokinase et son procédé de production Download PDF

Info

Publication number
WO2008136014A1
WO2008136014A1 PCT/IN2007/000277 IN2007000277W WO2008136014A1 WO 2008136014 A1 WO2008136014 A1 WO 2008136014A1 IN 2007000277 W IN2007000277 W IN 2007000277W WO 2008136014 A1 WO2008136014 A1 WO 2008136014A1
Authority
WO
WIPO (PCT)
Prior art keywords
enterokinase
seq
set forth
expression
amino acid
Prior art date
Application number
PCT/IN2007/000277
Other languages
English (en)
Inventor
Dipanwita Maiti
Anjali Chutke
Priti Khatri
Monsur Ahmed
Praveen Muneshwar
Niren Thakar
K. Meera Sankarankutty
Shrikant Mishra
Original Assignee
Usv Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Usv Limited filed Critical Usv Limited
Publication of WO2008136014A1 publication Critical patent/WO2008136014A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention porte sur le clonage et l'expression d'une nouvelle séquence d'acide nucléique codant pour l'entérokinase, l'expression ayant été effectuée dans un environnement positif de protéases. L'invention porte également sur un processus amélioré de production d'entérokinases sous la forme de corps d'inclusion de forme active. L'activité spécifique de l'entérokinase produite par la méthode de l'invention est au moins comparable à celle de l'entérokinase disponible dans le commerce.
PCT/IN2007/000277 2007-05-07 2007-07-06 Clonage et expression de l'entérokinase et son procédé de production WO2008136014A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN869MU2007 2007-05-07
IN869/MUM/2007 2007-05-07

Publications (1)

Publication Number Publication Date
WO2008136014A1 true WO2008136014A1 (fr) 2008-11-13

Family

ID=39242791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2007/000277 WO2008136014A1 (fr) 2007-05-07 2007-07-06 Clonage et expression de l'entérokinase et son procédé de production

Country Status (1)

Country Link
WO (1) WO2008136014A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012071257A1 (fr) * 2010-11-23 2012-05-31 Allergan, Inc. Compositions et procédés de production d'entérokinase dans des levures
US9611466B2 (en) 2011-12-23 2017-04-04 Novo Nordisk A/S Modified enterokinase light chain

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665566A (en) * 1993-01-15 1997-09-09 Genetics Institute, Inc. Cloning of enterokinase and method of use
CN1332248A (zh) * 2001-07-10 2002-01-23 南京大学 黄牛肠激酶催化亚基基因及其基因工程生产方法
US6342362B1 (en) * 1999-08-05 2002-01-29 Daniel S. Mytelka Methods and compositions for the purification of proteins or other macromolecules
WO2006001027A1 (fr) * 2004-06-23 2006-01-05 Usv Limited Hormone de croissance humaine chimerique derivee de placenta et d'isoformes hypophysaires et processus permettant d'obtenir cette chimere

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665566A (en) * 1993-01-15 1997-09-09 Genetics Institute, Inc. Cloning of enterokinase and method of use
US6342362B1 (en) * 1999-08-05 2002-01-29 Daniel S. Mytelka Methods and compositions for the purification of proteins or other macromolecules
CN1332248A (zh) * 2001-07-10 2002-01-23 南京大学 黄牛肠激酶催化亚基基因及其基因工程生产方法
WO2006001027A1 (fr) * 2004-06-23 2006-01-05 Usv Limited Hormone de croissance humaine chimerique derivee de placenta et d'isoformes hypophysaires et processus permettant d'obtenir cette chimere

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOI ET AL.: "Recombinant enterokinase light chain with affinity tag: Expression from Saccharomyces cerevisae and its utilities in fusion protein technology", BIOTECHNOLOGY AND BIOENGINEERING, vol. 75, no. 6, 20 December 2001 (2001-12-20), pages 719 - 724, XP002475276 *
Y. KITAMOTO ET AL.: "Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of distinctive assortment of domains", P.N.A.S., vol. 91, August 1994 (1994-08-01), pages 7588 - 7592, XP002475277 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012071257A1 (fr) * 2010-11-23 2012-05-31 Allergan, Inc. Compositions et procédés de production d'entérokinase dans des levures
RU2560577C2 (ru) * 2010-11-23 2015-08-20 Аллерган, Инк. Композиции и способы получения энтерокиназы в дрожжах
KR101920094B1 (ko) 2010-11-23 2018-11-19 알러간, 인코포레이티드 효모에서 엔테로키나아제를 생산하는 조성물과 방법
US9611466B2 (en) 2011-12-23 2017-04-04 Novo Nordisk A/S Modified enterokinase light chain

Similar Documents

Publication Publication Date Title
Panda Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli
Marston et al. [20] Solubilization of protein aggregates
Patra et al. Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli
US9738692B2 (en) Process for production of recombinant proteins as a soluble form
AU607973B2 (en) Process for production of physiologically active peptide containing cysteine residue
JPH11503002A (ja) 酵素的に活性な組換えカルボキシペプチダーゼｂの製造
EP2507258B1 (fr) Nouvelles peptidyl a-hydroxyglycine a-amide lyases
WO2010062279A1 (fr) Procédé de production d’insuline humaine recombinante
JP4239412B2 (ja) トランスグルタミナーゼの製造方法
JPH11514530A (ja) （２．５―ｄｋｇ）リダクターゼの各種改良型変異体
CN106701813A (zh) 表达载体及其构建方法与应用
WO2008136014A1 (fr) Clonage et expression de l'entérokinase et son procédé de production
JP5347255B2 (ja) 組換え蛋白質の可溶性蛋白質としての製造方法
CA2467142C (fr) Methode amelioree pour la production recombinante de polypeptides
US7189811B2 (en) Process for solubilization of recombinant proteins expressed as inclusion body
JP2000504561A (ja) Ｉｌ―１６活性のあるポリペプチド類、その製造方法およびその使用
GB2438952A (en) Production of proteins using secretory signal peptides
JP2020522999A (ja) グルコースオキシダーゼＣｎＧＯＤＡ及びその遺伝子ならびに使用
CA3086618A1 (fr) Gene precurseur a codon optimise et gene de peptide signal de l'analogue de l'insuline humaine
CN116478969A (zh) 胶原酶突变体、促进重组胶原酶分泌表达的方法及其应用
WO2007068053A1 (fr) Production de protéines par voie recombinante
FI103132B (fi) Menetelmä rekombinantti-IgA-proteaasin valmistamiseksi
WO1988005816A1 (fr) Polypeptide
WO1990007578A1 (fr) Proteines hormonales de poisson de recombinaison
US20060035316A1 (en) Plasmids expressing human insulin and the preparation method for human insuling thereby

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07849677

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07849677

Country of ref document: EP

Kind code of ref document: A1