WO2008047658A1 - Additif pour aliments pour animaux et aliments pour animaux - Google Patents
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- WO2008047658A1 WO2008047658A1 PCT/JP2007/069807 JP2007069807W WO2008047658A1 WO 2008047658 A1 WO2008047658 A1 WO 2008047658A1 JP 2007069807 W JP2007069807 W JP 2007069807W WO 2008047658 A1 WO2008047658 A1 WO 2008047658A1
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- Prior art keywords
- feed additive
- feed
- mel
- rhamnolipid
- additive according
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7032—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a polyol, i.e. compounds having two or more free or esterified hydroxy groups, including the hydroxy group involved in the glycosidic linkage, e.g. monoglucosyldiacylglycerides, lactobionic acid, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Definitions
- the present invention relates to a feed additive containing glycolipid, a feed, and a method for breeding birds and mammals using the same.
- Staphylococcus aureus is a causative agent of urchin, hidge, goat mastitis, subcutaneous tumor, abscess, equine rash, pig, chicken arthritis, dermatitis, sepsis is there.
- Streptococcus suis (Strept ococcus suis) is a causative agent of meningitis, sepsis, endocarditis and arthritis in swine
- Streptococcus bovis is a causative agent of pulmonary tympanosis. It is.
- MRSA methicillin-resistant Staphylococcus aureus
- MRSA methicillin-resistant Staphylococcus aureus
- polypeptides such as nisin produced by lactic acid bacteria and iturin produced by Bacillus bacteria are used instead of antibiotics.
- sucrose esters which are glycolipids added to canned coffee and the like as an emulsifier, are added in anticipation of antibacterial action against Bacillus bacteria.
- ruminant livestock such as cattle and sheep are digested and fermented with microorganisms in the lumen to live using the fermented products. Therefore, methane generation from the lumen results in a loss of feed energy efficiency. Furthermore, since methane is a warming gas that affects global warming, it is important to reduce methane production in ruminant rumen.
- Methanogens in the rumen use hydrogen to reduce carbon dioxide and produce methane.
- the contribution rate of methane to global warming is estimated to account for 15 to 20% of methane released from ruminant livestock out of the total amount of methane released next to carbon dioxide.
- Ionophores such as monensin, which are antibiotics, are widely used in ruminant feeds.
- Monensin has a selective inhibitory effect on rumen microorganisms, and as a result, has a function of reducing methane production and promoting propionic acid production.
- Propionic acid has a higher ATP production efficiency than other volatile fatty acids! /, Thus improving the feed efficiency by promoting the production of propionic acid.
- Non-patent document 1 plant extract oil
- Non-patent document 2 anti-lactic acid-producing bacteria vaccine
- Non-patent document 3 anti-lactic acid-producing bacteria egg antibody
- glycolipids such as mannosyl erythritol lipid (MEU and rhamnolipid (RU) have various properties including surface-active action, and can be developed for various uses as described below.
- MEU mannosyl erythritol lipid
- RU rhamnolipid
- Patent Document 1 a technology that improves the efficiency of gene introduction using ribosomes containing MEL (Patent Document 1), and inhibits the formation of ribosomes containing genes such as drug resistance using MEL, thereby Methods for reducing the occurrence of fungi, etc.
- Patent Document 2 technology using MEL as an active ingredient of anti-inflammatory agents and anti-allergic agents
- Patent Document 3 also known are natural fibers using rhamnolipids.
- Patent Document 4 Technology for improving water absorption
- Patent Document 5 non-treatment containing rhamnolipids and harmful organic compounds
- Patent Document 5 a technology for preventing ice agglomeration and coalescence by preparing ice slurry for high-density cold heat storage transport using rhamnolipid
- Patent Document 5 a technology for preventing ice agglomeration and coalescence by preparing ice slurry for high-density cold heat storage transport using rhamnolipid
- Patent Document 5 Although some antibacterial properties of MEL and rhamnolipid have been reported (Non-patent document 4, Non-patent document 5), antibacterial activity against bacteria causing infectious diseases in livestock has not yet been studied. There are no examples of applying MEL or rhamnolipid in the field of livestock.
- Patent Document 1 Japanese Patent Application Laid-Open No. 2006-174727
- Patent Document 2 JP 2006-158387 A
- Patent Document 3 Japanese Patent Laid-Open No. 2005-68015
- Patent Document 4 JP-A-2002-105854
- Patent Document 5 Japanese Patent Laid-Open No. 2001-327803
- Patent Document 6 Japanese Patent Laid-Open No. 2001_131538
- Non-patent literature l Benchaar et al., Can. J. Anim. Sci. 86, 91-96 (2006)
- Non-Patent Document 2 Shu et al., FEMS Immunology & Medical Microbiology, 26 (2), 153-15
- Non-Patent Document 3 DiLorenzo et al., J. Anim. Sci., 84, 2178-2185 (2006)
- Non-Patent Document 4 Fat. Sci. Technol., 91, 363-366, 1989
- Non-Patent Document 5 Biotechnol., 29, 91-96, 1993
- An object of the present invention is to provide a safe and simple means for preventing or treating diseases of birds and mammals, particularly livestock.
- the subject of the present invention is in particular to provide a means for preventing or treating livestock infections caused by gram-positive bacteria.
- Another object of the present invention is to improve rumen fermentation of ruminants, contribute to suppression of generation of greenhouse gases, and further improve feed efficiency.
- glycolipids such as mannosyl erythritol lipid (MEL) and rhamnolipid (RL) cause infectious diseases in livestock.
- MEL mannosyl erythritol lipid
- RL rhamnolipid
- the present invention is as follows.
- a feed additive for birds or mammals containing mannosylerythritol lipid and / or rhamnolipid containing mannosylerythritol lipid and / or rhamnolipid.
- Mannosyl erythritol lipid strength The feed additive according to any one of (1) to (4), which is obtained from yeast strength belonging to the genus Pseudozyma.
- Gram-positive bacteria are Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus suis, or Streptococcus vis. The feed additive as described in 9).
- a feed comprising the feed additive according to any one of (1) to (; 10).
- Fig. 1 shows the effect of RL and MEL lumens on gas production and composition. Indicates.
- FIG. 2 shows the effect on the concentration and ratio of volatile fatty acids in RL and MEL lumens.
- the feed additive of the present invention is characterized by containing mannosyl erythritol lipid (MEU and / or rhamno lipid (RL)).
- MEU mannosyl erythritol lipid
- RL rhamno lipid
- MEL is a kind of glycolipid type biosurfactant, and has a structure in which mannose, erythritol, and fatty acid are bound, and is represented by the following general formula (1).
- R 1 and R 2 are each independently an aliphatic acyl group having 3 to 25 carbon atoms.
- R 1 and R 2 are preferably each independently an aliphatic acyl group having 5 to 14 carbon atoms.
- R 1 and R 2 may each independently be an aliphatic acyl group having 5 to 13 carbon atoms.
- These aliphatic acyl groups may be linear or branched, saturated or unsaturated.
- One of R 3 and R 4 is a acetyl group and the other is hydrogen, or both are acetyl groups.
- MEL-A In the case where R 3 and R 4 are both acetyl groups, MEL-A, R 3 is hydrogen, R 4 is acetyl group, MEL-B, R 3 is acetyl group, R 4 is M is hydrogen Called EL—C.
- the MEL in the feed additive of the present invention may be one kind or a mixture of plural kinds.
- the MEL used in the present invention can be obtained by culturing microorganisms such as fungi, particularly yeasts.
- yeasts belonging to the genera Pseudozyma, Candida, and Kurtzmanomyces can be used. Shizonell a melanogramma can also be used.
- Pseudozyma aphidis, Pseudozyma antarctica, etc. are mentioned as yeast which belongs to the genus Syuduzaima.
- Pseudozyma aphidis NBRC dish 82 strain Peudozyma Antarctica NBRC 10260 strain, Peudozyma Antarctica NBRC 10736 strain can be used.
- NBRC dish 82 strain The NBRC dish 82 strain, NBRC 10260 strain, and NBRC 10736 strain are registered in the Biological Genetic Resource Department (NBRC) of the National Institute of Technology and Evaluation. In addition, a synthesized or commercially available MEL can be used.
- Rhamnolipid is a type of glycolipid biosurfactant and has a structure in which rhamnose and fatty acid are combined.
- the rhamnolipid used in the present invention is not particularly limited, but for example, a compound having a structure represented by the following general formula (2) or general formula (3) can be used.
- R is a hydrogen atom, —CH— [CH (OH)] —CH (OH), 1 (
- the alkyl group and the alkenyl group may be linear or branched.
- the aliphatic acyl group may be linear, branched or saturated. It may be saturated.
- M is an integer of 0 to 8
- X is at least one of ethylene, propylene and butylene
- n is an integer of 1 to 1000.
- R 6 is a hydrogen atom or 2-dece group. R 5 and R 6 are independent.
- R 7 is a hydrogen atom, CH— [CH (OH)] —CH (OH), 1 (
- XO XO
- H or an alkyl group, alkenyl group or aliphatic acyl group having 1 to 36 carbon atoms Show.
- the alkyl group and the alkenyl group may be linear or branched.
- the aliphatic acyl group may be linear, branched or saturated. It may be saturated.
- M is an integer of 0 to 8
- X is at least one of ethylene, propylene and butylene
- n is an integer of 1 to 1000.
- R 8 is a hydrogen atom or a 2-decenoyl group.
- R 7 and R 8 are independent.
- the rhamnolipid in the feed additive of the present invention may be only one kind or a mixture of plural kinds.
- the rhamnolipid used in the present invention can be obtained by culturing bacteria.
- bacteria belonging to the genus Pseudomonas and Burkholderia can be used.
- bacteria belonging to the genus Pseudomonas Pseudomonas aeruginosa ⁇ Pseudomonas chlororaphis isobaric, Pseudomonas sp., Pseudomonas sp. Can be used as a shoot moth.
- Examples of the bacteria belonging to the genus Nordhorderia include Burkholderia pseudomalle.
- Pseudomonas aeruginosa it is particularly preferable to use Pseudomonas aeruginosa.
- Pseudomonas aeruginosa NBRC 3924 strain, Pseudomonas sp. DSM 2874 strain and the like can be used.
- the NBRC 3924 strain is a strain registered in the Biogenetic Resources Department (NBRC) of the National Institute of Product Evaluation Technology.
- the DSM 2874 strain is a strain registered with Deutsche Sammlung von Mikroorganismen una Zellkulturen GmbH (DSMZ)!
- the rhamnolipid can also use what was synthesize
- raw materials such as natural fats and oils, fatty acids, alcohols, ketones, hydrocarbons, n-alkanes, etc.
- raw materials such as natural fats and oils, fatty acids, alcohols, ketones, hydrocarbons, n-alkanes, etc.
- Is natural oils and fats such as soybean oil, sunflower oil, coconut oil, cottonseed oil, corn oil, palm oil, etc.
- soybean oil is particularly preferred. It is done.
- a raw material suitable for the microorganism to be used is selected from raw materials such as natural fats and oils, fatty acids, alcohols, ketons, hydrocarbons, n-alkanes and sugars, and the microorganisms are selected. What is necessary is just to culture
- the culture method is not particularly limited, and it is possible to use a liquid culture method or a solid culture method such as a stationary culture, a reciprocating shake culture, a rotary shake culture, or a jar mentor culture. I'll do it.
- the culture may be purified, and a purified product of MEL and / or rhamnolipid may be used, or the culture may be centrifuged, A fraction containing rhamnolipid may also be used.
- the culture may be used as it is. For example, a culture solution or a solid culture obtained by pulverizing for 4 minutes can be used.
- the feed additive of the present invention may contain either MEL or rhamnolipid, or both of them.
- the content of MEL and / or rhamnolipid is not particularly limited, but is preferably 10 ppm by mass or more, more preferably 1% by mass or more, from the viewpoint of obtaining sufficient effects.
- the feed additive of the present invention is an ingredient effective for preventing or treating avian or mammal diseases, an ingredient effective for promoting the growth of ruminants, and nutrition. It may further contain optional components such as auxiliary components and components that enhance storage stability.
- Such optional components include enterococcus, bacillus, bifido Viable agents such as fungi; enzymes such as amylase and lipase; vitamins such as L-ascorbic acid, choline chloride, inositol, and folic acid; minerals such as potassium chloride, iron citrate, magnesium oxide, phosphates, DL-alanine, Amino acids such as DL-methionine and L-lysine hydrochloride; organic acids such as fumaric acid, butyric acid, lactic acid, acetic acid and salts thereof; antioxidants such as ethoxyquin and dibutylhydroxytoluene; antifungal agents such as calcium propionate; Binders such as CMC, sodium caseinate and sodium polyacrylate; emulsifiers such as glycerin fatty acid ester and sorbitan fatty acid ester; pigments such as fastaxanthin and canthaxanthin; flavors such as various esters,
- the dosage form of the feed additive of the present invention is not particularly limited, and can be any form such as powder, liquid, tablet, and the like.
- the feed additive of the present invention can be produced by mixing and formulating MEL and / or rhamnolipid and optional components as necessary.
- the feed additive of the present invention can prevent diseases of birds or mammals caused by these bacteria. Can be used to prevent or treat.
- the feed additive of the present invention can be suitably used particularly for the prevention or treatment of infectious diseases caused by Gram-positive bacteria.
- Examples of such gram-positive bacteria include Micrococcus, Staphylococcus, Strepto coccus, Planococcus, ⁇ tomatococcus ⁇ terococcus, Peptococcus, Peptostreptococ cus, Ruminococcus, Leuconostoc ⁇ Psdiococcus, Asrococcus, Gemella ⁇ Coprococtris L, ⁇ Bacteria belonging to genera such as Erysipelothrix, Corynebacterium, Rhodococcus, Propionibacterium ⁇ Eubacterium, Actinomyces ⁇ Bifidobacterium, Mycobacterium, Nocardia, Dermatophilus.
- the feed additive of the present invention is particularly suitable for the prevention or treatment of diseases caused by bacteria belonging to the genus Staphylococcus or Streptococcus, specifically (such as Staphylococcus aureus staphylococcus epidermidis, ⁇ treptococcus cus suis, Streptococcus bovis, etc. Use with power S.
- the feed additive of the present invention is mixed with other feed ingredients used for avian or mammalian feed, pet food, pet supplements (hereinafter referred to as feed), and is used for birds or mammals. Can be used as feed. There are no particular restrictions on the type and ingredients of the feed. Further, the feed of the present invention may be prepared by adding the above-mentioned optional components that can be added to the feed additive. Further, the feed of the present invention can be used as a feed for preventing or treating diseases of birds or mammals.
- the content of MEL and / or rhamnolipid in the feed of the present invention is appropriately adjusted depending on the type of animal to be given, health condition, type of feed, feed ingredient, age, sex, body weight, etc., and is not particularly limited.
- Per mass preferably 1 to; 10000 ppm by mass, more preferably ⁇ 10 to; 10000 ppm by mass, more preferably ⁇ 10 to 1000 ppm.
- the feed of the present invention can be produced by adding a feed additive to a feed component as it is and mixing them.
- the feed additive may be in a liquid or gel form for easy mixing.
- water, vegetable oils such as soybean oil, rapeseed oil and corn oil, liquid animal oils, water-soluble polymer compounds such as polybulal alcohol, polybulpyridone and polyacrylic acid can be used as the liquid carrier.
- a water-soluble polysaccharide such as alginic acid, sodium alginate, xanthan gum, sodium caseinate, gum arabic, guar gum, tamarind seed polysaccharide.
- the kind of animal to be fed the feed of the present invention is a bird or a mammal.
- it can be used for pets such as livestock, dogs and cats.
- the feed of the present invention is suitable for raising livestock, particularly chickens, pigs and cows. It is also suitable for breeding ruminants.
- the feed of the present invention is suitable for breeding cows, goats, sheep and the like.
- the amount of feed to be ingested can be adjusted as appropriate according to the type of animal, body weight, age, sex, health status, ingredients of the feed, and the like.
- a commonly used method can be used depending on the kind of animal.
- a crude product diluted to an appropriate concentration with methanol was placed in a test tube, and the solvent was distilled off. Thereto was added 5 ml of anthrone reagent (0.2% anthrone 75% sulfuric acid solution), reacted in boiling water for 10 minutes, and the absorption at 620 nm was measured. The purity of the crude product was calculated by comparison with the standard sample.
- a bouillon medium (Nissui) for sensitivity measurement.
- the bacterial concentration of the culture solution was adjusted to about 1.
- OX 10 5 to 10 6 CFU / ml with physiological saline and then various bacteria were inoculated into the measurement medium.
- the sensitivity measurement medium (Nissui) was used for Staphylococcus aureus, Stap hylococcus epidermidis, and Bacillus subtilis, and the Jki night agar medium (Nountoinfusion medium: Nissui, for Streptococcus suis and Streptococcus bovis). Aseptic defibrinated blood of sheep: Kojin Bio).
- Culturing was carried out preferably for Stap nylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and 5% carbon dioxide for Streptococcus suis and Streptococcus bovis, both at 37 ° C. for about 20 hours. After completion of the culture, MIC was measured.
- MEL the crude product of MEL obtained in ⁇ 1> (purity 69%) was used, and for rhamnolipid, the crude product of rhamnolipid obtained in ⁇ 2> (purity 55%) was used.
- MICs were also measured for sucrose monodecanoate (Sucrose monodecanoate), mannose (Rokko Pure Chemical Industries), and rhamnose (Sigma Aldrich Japan). Is shown in Table 1.
- MEL and rhamnolipid exhibited antibacterial activity several to several tens of times higher against bacteria of the genus Staphylococcus, Streptococcus, and Bacillus, compared to Sucrose monodecanoate, which is the same glycolipid.
- mannose and rhamnose which are glycolipid constituents, did not show antibacterial activity.
- MEL and rhamnolipid are expected to have an effect of preventing or treating diseases caused by the above-mentioned various bacteria by ingesting and raising birds and mammals.
- MEL Mannosyl erythritol lipid
- the crude product diluted to an appropriate concentration with ethyl acetate was placed in a test tube, and the solvent was distilled off. Thereto was added 5 ml of anthrone reagent (0.2% anthrone 75% sulfuric acid solution) and reacted in boiling water for 10 minutes, and the absorption at 620 kg was measured. The purity of the crude product was calculated by comparison with the standard sample.
- the rhamnolipid (RU and mannosyl erythritol lipid (MEU) were used.
- the cultured inoculum was a Holstein heifer (with a lumen cannula) owned by the Hokkaido University Northern Biosphere Field Science Center Biological Production Research Farm)
- the inoculum was diluted 2-fold with McDougal's artificial saliva (pH 6.8).
- the test medium concentration was 500 ⁇ g / ml for RL and 500 ⁇ g / ml for MEL.
- 0.05 g each of RL and MEL was dissolved in 1 ml of ethanol, and 100 1 each was added to the Hangate tube. The ethanol was volatilized by leaving it for several hours.
- 0-15 g of corn starch, 0.025 g of mixed feed powder and 0.025 g of orchardgrass hay powder were added as culture substrates. 10 ml of the above diluted lumen solution was added, a butyl rubber cap and a plastic screw cap were applied while blowing nitrogen gas into the head space, and anaerobic culture was performed in a water bath (37 ° C, 18 hours).
- the treatment was not added (ethanol only: control group), RL added (RLE) and MEL added (MEL group), and each of them was cultured in 5 series.
- Methane, hydrogen and carbon dioxide were analyzed by TCD gas chromatography.
- Total volatile fatty acid (VFA) concentration and composition were determined by FID gas chromatography.
- the disease can be prevented or treated by mixing the feed additive of the present invention with the feed and ingesting it to birds or mammals. Specifically, infections caused by gram-positive bacteria can be prevented or treated.
- the feed containing the feed additive of the present invention can be suitably used for raising livestock such as chickens, pigs and cows.
- the feed additive of the present invention is highly biodegradable and highly safe for living bodies and the environment.
- the feed additive of the present invention by mixing the feed additive of the present invention with the feed and ingesting the ruminant animal, it is possible to suppress methan production and promote propionic acid production, and as a result, the growth of ruminant animals can be promoted. Promote and improve feed efficiency.
- the feed containing the feed additive of the present invention can be suitably used for breeding ruminants such as cattle, goats and sheep.
- the feed additive of the present invention is highly biodegradable and highly safe for living bodies and the environment.
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Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2007313580A AU2007313580B8 (en) | 2006-10-17 | 2007-10-11 | Animal feed additive and animal feed |
US12/444,034 US20100249058A1 (en) | 2006-10-17 | 2007-10-11 | Feed additive and feed |
CA2666674A CA2666674C (en) | 2006-10-17 | 2007-10-11 | Feed additive and feed |
EP07829545.8A EP2074889A4 (en) | 2006-10-17 | 2007-10-11 | ANIMAL FEED ADDITIVE AND ANIMAL FEED |
NZ575806A NZ575806A (en) | 2006-10-17 | 2007-10-11 | Animal feed comprising mannosylerythritol and rhamnolipds |
BRPI0717628-7A2A BRPI0717628A2 (pt) | 2006-10-17 | 2007-10-11 | Aditivo de ração para aves e mamíferos, ração, e, método para criar aves ou mamíferos |
MX2009003416A MX2009003416A (es) | 2006-10-17 | 2007-10-11 | Aditivo de alimento de animales y alimento de animales. |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006-282697 | 2006-10-17 | ||
JP2006282697 | 2006-10-17 | ||
JP2007144393 | 2007-05-31 | ||
JP2007-144393 | 2007-05-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2008047658A1 true WO2008047658A1 (fr) | 2008-04-24 |
Family
ID=39313896
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2007/069807 WO2008047658A1 (fr) | 2006-10-17 | 2007-10-11 | Additif pour aliments pour animaux et aliments pour animaux |
Country Status (12)
Country | Link |
---|---|
US (1) | US20100249058A1 (ja) |
EP (1) | EP2074889A4 (ja) |
KR (1) | KR20090079238A (ja) |
AR (1) | AR063475A1 (ja) |
AU (1) | AU2007313580B8 (ja) |
BR (1) | BRPI0717628A2 (ja) |
CA (1) | CA2666674C (ja) |
MX (1) | MX2009003416A (ja) |
MY (1) | MY142794A (ja) |
NZ (1) | NZ575806A (ja) |
TW (1) | TW200824577A (ja) |
WO (1) | WO2008047658A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2011056871A2 (en) * | 2009-11-03 | 2011-05-12 | Keith Desanto | Rhamnolipid mechanism |
JP2013094153A (ja) * | 2011-11-04 | 2013-05-20 | Hiroshima Prefecture | 水素生産用の添加剤及びその添加剤を用いた水素生産方法 |
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WO2015143169A1 (en) * | 2014-03-20 | 2015-09-24 | The University Of Akron | Novel materials derived from fermentation-produced rhamnolipids |
ES2966909T3 (es) | 2014-08-13 | 2024-04-25 | Akeso Biomedical Inc | Compuestos y composiciones antimicrobianos, y usos de los mismos |
US10653658B2 (en) | 2015-08-11 | 2020-05-19 | Akeso Biomedical, Inc. | Biofilm inhibiting compositions enhancing weight gain in livestock |
PL3334440T3 (pl) | 2015-08-11 | 2021-11-02 | Akeso Biomedical, Inc. | Kompozycje hamujące tworzenie biofilmu wspierające przyrost masy ciała u zwierząt gospodarskich |
WO2018123680A1 (ja) | 2016-12-27 | 2018-07-05 | 国立大学法人広島大学 | バイオサーファクタントの利用 |
EP3612021A4 (en) * | 2017-04-20 | 2021-04-14 | Locus IP Company, LLC | COST-EFFECTIVE COMPOSITIONS AND PROCESSES TO IMPROVE AQUACULTURE AND AQUARIUM FISH BREEDING |
AU2019356502A1 (en) * | 2018-10-09 | 2021-04-08 | Locus Ip Company, Llc | Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases |
EP3639675A1 (en) | 2018-10-19 | 2020-04-22 | EW Nutrition GmbH | Rhamnolipid compositions, an animal feed or additive comprising the compositions, a method of feeding monogastric animals and the use of the composition for digestibility and/or absorption enhancement |
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- 2007-10-11 EP EP07829545.8A patent/EP2074889A4/en not_active Withdrawn
- 2007-10-11 AU AU2007313580A patent/AU2007313580B8/en not_active Ceased
- 2007-10-11 MY MYPI20091554A patent/MY142794A/en unknown
- 2007-10-11 US US12/444,034 patent/US20100249058A1/en not_active Abandoned
- 2007-10-11 CA CA2666674A patent/CA2666674C/en not_active Expired - Fee Related
- 2007-10-11 BR BRPI0717628-7A2A patent/BRPI0717628A2/pt not_active IP Right Cessation
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011056871A2 (en) * | 2009-11-03 | 2011-05-12 | Keith Desanto | Rhamnolipid mechanism |
WO2011056871A3 (en) * | 2009-11-03 | 2011-10-20 | Keith Desanto | Rhamnolipid mechanism |
JP2013094153A (ja) * | 2011-11-04 | 2013-05-20 | Hiroshima Prefecture | 水素生産用の添加剤及びその添加剤を用いた水素生産方法 |
Also Published As
Publication number | Publication date |
---|---|
EP2074889A1 (en) | 2009-07-01 |
EP2074889A4 (en) | 2013-10-30 |
MY142794A (en) | 2010-12-31 |
KR20090079238A (ko) | 2009-07-21 |
AR063475A1 (es) | 2009-01-28 |
AU2007313580B8 (en) | 2010-08-26 |
NZ575806A (en) | 2011-09-30 |
US20100249058A1 (en) | 2010-09-30 |
MX2009003416A (es) | 2009-04-09 |
AU2007313580A1 (en) | 2008-04-24 |
CA2666674A1 (en) | 2008-04-24 |
TW200824577A (en) | 2008-06-16 |
CA2666674C (en) | 2011-05-31 |
BRPI0717628A2 (pt) | 2013-10-29 |
AU2007313580B2 (en) | 2010-07-29 |
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