WO2007132740A1 - 反応容器キット - Google Patents
反応容器キット Download PDFInfo
- Publication number
- WO2007132740A1 WO2007132740A1 PCT/JP2007/059687 JP2007059687W WO2007132740A1 WO 2007132740 A1 WO2007132740 A1 WO 2007132740A1 JP 2007059687 W JP2007059687 W JP 2007059687W WO 2007132740 A1 WO2007132740 A1 WO 2007132740A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- reaction
- sample
- reaction container
- container
- cover
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/54—Labware with identification means
- B01L3/545—Labware with identification means for laboratory containers
- B01L3/5457—Labware with identification means for laboratory containers for container closures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/021—Identification, e.g. bar codes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N2001/002—Devices for supplying or distributing samples to an analysing apparatus
- G01N2001/005—Packages for mailing or similar transport of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00861—Identification of carriers, materials or components in automatic analysers printing and sticking of identifiers
Definitions
- the present invention relates to a reaction container kit suitable for performing various analyzes and analyzes in the field of medical treatment or chemistry in the fields of biological analysis, biochemical analysis, or chemical analysis in general. .
- a micro multi-chamber apparatus is used as a small reaction apparatus used for biochemical analysis and normal chemical analysis.
- a microwell reaction plate such as a microtiter plate in which a plurality of wells are formed on a flat substrate surface is used as a reaction vessel.
- reaction container a reaction container including a reaction part that causes a sample to react and a reagent container that contains a reagent used for the reaction of the sample has been proposed as a reagent kit.
- Reagents corresponding to sample inspection items are selected in advance and stored in the reagent container! Speak.
- reaction vessel it is difficult to distinguish whether it is a reaction vessel in which a sample is not injected or a reaction vessel in which a sample is injected.
- An object of the present invention is to prevent a mistaken reaction container into which a sample is to be injected and to easily determine whether the sample is before or after sample injection. .
- the reaction container kit of the present invention comprises a reaction part for causing a sample to react and a reagent container containing a reagent used for the reaction of the sample, and before dispensing the sample into the reaction container.
- a first barcode label for reading and a second barcode label for reading after dispensing the sample into the reaction vessel is affixed to the reaction container in advance, and the second bar code label is arranged so that it can be affixed to the reaction container.
- the first bar code label and the second bar code label Different data is recorded on the label, and the first bar code label may contain at least data indicating information specific to the reaction vessel.
- the first barcode label is read by a barcode reader, and it is automatically determined whether or not the reaction vessel is a reaction vessel for the inspection item requested for the sample to be injected. To do.
- the first barcode label still has a sample injected into its reaction vessel.
- Data indicating V, etc. can be recorded, and data indicating that a sample has already been injected into the reaction vessel can be recorded on the second barcode label. If the barcode label affixed to the reaction container is the first barcode label, the sample is still injected into the reaction container by reading the barcode label with a barcode reader. If the barcode label affixed to the reaction vessel is the second barcode label, the sample is already injected into the reaction vessel by reading the barcode label with a barcode reader. It can be determined that
- a preferred form for preventing the first barcode label from being left on the reaction container after the sample has been injected is that the first barcode label is completely or after being read. Some are peeled off.
- a portion of the first barcode label that remains attached to the reaction container without being peeled off is, for example, Record data indicating information specific to the reaction vessel, such as the items to be inspected in the reaction vessel, and that no sample has been injected into the reaction vessel yet to be removed with the first barcode label. Record the data indicating the The bell can be recorded with data indicating that the sample has already been injected into the reaction vessel.
- the reaction container has an opening serving as a sample introduction part
- the first barcode label is attached to the sample introduction part so that the opening cannot be opened unless the part to be peeled off of the barcode label is peeled off.
- the second barcode label also serves as a sealing member for sealing the opening after the sample is injected.
- reaction container kit of the present invention is designed to prevent the entry of foreign matter with external force and environmental pollution to the outside.
- reaction container kit is a reaction plate having a reaction part and a reagent container on the surface side, a dispensing chip arranged above the surface side of the reaction plate, and a reaction plate A cover that covers the upper space on the surface side, and supports the dispensing tip so that the dispensing tip is movably supported with the tip end inside the space and the base end outside.
- the part is provided in a part of the cover, and the sample introduction part is configured to inject the sample into the space from the outside through the opening.
- a preferred embodiment of the reaction plate has a reagent container on the surface side, and the reagent container is sealed with a film.
- the film that covers the reagent container and seals the reagent can be penetrated with a dispensing tip.
- reaction container is disposable.
- the dispensing tip may be attached to the tip of the dispensing nozzle. In that case Requires a nozzle mechanism for dispensing operation. Therefore, in order to eliminate the need for such a nozzle mechanism, in a preferred embodiment of the present invention, the dispensing tip is provided with a syringe operated from the outside of the cover, and the dispensing operation is performed by operating the syringe. It can be.
- the dispensing tip includes a syringe, since the syringe seals the passage of the dispensing tip, the inside and outside of the space covered with the cover will not be communicated via the passage of the dispensing tip.
- the dispensing tip is provided with a syringe, it can be sealed by a nozzle mechanism during dispensing operation, but the dispensing tip is used during reaction or detection. Sometimes, it communicates with the external space through the dispensing tip. Even in such a case, as a preferable form for preventing foreign matter from entering from the outside and preventing the sample and its reaction product from coming out, the dispensing tip is provided with a filter inside the tip. It can be done as a sword.
- the reaction plate is preferably provided with a gene amplification section for performing a gene amplification reaction on the surface side thereof.
- the gene amplification section preferably has a shape suitable for temperature control at a predetermined temperature cycle, and the reaction section can be formed into such a shape as a gene amplification section.
- An amplification container may be provided.
- Gene amplification reactions include PCR and LAMP.
- the analysis of the reaction product in the reaction vessel can be performed in the reaction section, or the reaction section force can be moved to another place on the reaction plate.
- the reaction part is preferably made of a light-transmitting material so that it can be optically measured from the bottom.
- the reaction plate In a reaction vessel in which the reaction product is analyzed by moving it from the reaction part to another place, the reaction plate has an analysis part for analyzing the reaction product in the reaction part on the surface side. It has more.
- An example of such an analysis unit is an electrophoresis unit that performs electrophoretic separation of reaction products.
- Another example of such an analysis unit is a region where a probe that reacts with a gene is arranged when a reaction product contains a gene. Examples of such a probe arrangement region are a DNA chip and a hybrid region.
- An example of a structure that holds and displaces the dispensing tip is a structure that holds and displaces the dispensing tip with an airtight and flexible material such as a diaphragm film.
- the cover is a rigid cover body integrated with the reaction plate, and is attached to the top of the reaction plate on the surface side of the cover plate.
- An opening in which the sample introduction part is disposed is provided in the cover body, and a sealing member for sealing the opening is attached to the cover body.
- Other examples of the structure that holds the dispensing tip and supports it in a movable manner include a cover body in which the cover is integrated with the reaction plate, and an upper portion on the surface side of the reaction plate that is sealed against the cover body.
- the cover plate is kept airtight by the material and slidably held in the horizontal plane, and the dispensing tip is slidable in the vertical direction while being airtight by the other sealing material on the cover plate. It is a held structure.
- the opening in which the sample introduction part is disposed is provided in the cover body, and the sealing member for sealing the opening is attached to the cover body.
- the reaction container kit of the present invention is used for measurement of various reactions including chemical reactions and biochemical reactions.
- Examples of the sample measured using the reaction container kit of the present invention include various substances such as chemical substances, biological samples, and biological samples, and are not particularly limited.
- a first barcode label for reading before sample dispensing is affixed to the reaction container in advance, and the first barcode label has information unique to the reaction container. Therefore, by reading the first bar code label with a bar code reader before sample injection, the reaction vessel force for the sample to be injected for the test item requested. Automatically determines whether or not the reaction vessel is power This can prevent the human error of selecting the reaction vessel by mistake.
- the reaction container By reading the barcode label affixed to the sample with a barcode reader, it is possible to determine whether or not the reaction container has already been injected with the sample. Inadvertent mistakes can be prevented by injecting the sample again into the reaction vessel before it is processed.
- the first barcode label remains attached to the reaction container even after the sample is injected.
- the bar code label will make it easier to determine whether or not a sample has been injected.
- the reaction vessel has an opening that serves as a sample introduction part, and the first barcode label is introduced so that the opening cannot be opened unless the part to be peeled off of the first barcode label is removed. If it is attached to the part, it can be surely prevented that the first barcode label remains attached to the reaction container even after the sample is injected.
- the second barcode label also serves as a sealing member that seals the opening after sample injection, the inside of the reaction vessel may be sealed with the second barcode label. As a result, no other sealing member for sealing the opening is required, which contributes to cost reduction.
- a reaction part and a reagent container are provided on the reaction plate surface side, the upper space on the surface side on the reaction plate is covered with a cover, and a sample introduction part opening is formed in a part of the cover. If the sample is injected into the space covered by the external force cover through the opening, the opening is opened when the sample is injected into the space covered by the cover. Sealing can prevent foreign substances from entering the sample from the outside, and can also prevent reaction products from contaminating the external environment.
- a dispensing tip that is movably supported by a cover that covers the upper surface side of the reaction plate is provided, and the dispensing tip includes a syringe that also operates the outer force of the cover, a nozzle is provided. There is no need to provide a separate mechanism.
- the reaction plate is further equipped with a gene amplification part, it contains only a very small amount of the gene to be measured! Even if it is a sample, the gene is amplified and analyzed by a gene amplification reaction such as PCR or LAMP. The accuracy can be increased.
- the dispensing tip is provided with a filter inside the tip, the dispensing tip should be provided with a syringe, and even if this is the case, an external force foreign matter can be prevented from entering through the dispensing tip. In addition, the reaction product can be prevented from contaminating the external environment through the dispensing tip.
- the gene amplification reaction is also performed in a closed space, and after the analysis is completed, it is disposed of in the closed space, so that it is possible to prevent contamination by external force and to contaminate other samples. Disappear.
- reaction products in the reaction vessel should be performed in the reaction unit, in an electrophoresis unit provided at a different location from the reaction unit, or in a probe placement region that reacts with a gene. If so, the types of samples to be handled can be expanded.
- the structure that holds and displaces the dispensing tip is realized by an airtight and flexible material, or the dispensing tip is attached to the cover body by using the cover as a force between the cover body and the cover plate. If the cover plate is slidably supported by sliding the cover plate and the dispensing chip with respect to the cover plate, the structure for holding the dispensing tip and supporting it movably can be realized with a simple configuration. it can.
- FIG. 1 Fig. 1 (A) to Fig. 1 (C) are external perspective views of a reaction container kit of one embodiment, and Fig. 1 (A) is a state before a sample is injected, Fig. 1 (B) shows the state where the first bar code label has been removed to inject the sample, and FIG. 1 (C) shows the state where the second bar code label has been applied after the sample has been injected.
- FIG. 2 Fig. 2 (A) to Fig. 2 (C) show the internal structure of the same example, Fig. 2 (A) is a vertical cross-sectional view, and Fig. 2 (B) is a reaction plate and a separation plate.
- FIG. 2C is a schematic cross-sectional view showing another example of a dispensing tip.
- FIG. 3 is a vertical sectional view showing a state where the sample is introduced in the embodiment.
- FIG. 4 is a vertical sectional view showing a state in which the syringe drive unit of the drive unit is engaged with the plunger of the syringe in the same embodiment.
- FIG. 5 is a vertical sectional view showing a state where the tip holding portion of the drive unit is engaged with the dispensing tip in the same example.
- FIG. 6 is a vertical cross-sectional view showing a state in which the dispensing tip is also removed from the dispensing tip in the same embodiment.
- FIG. 7 is a vertical sectional view showing a first example of a detection unit used for detecting a reaction product in the reaction container kit of the present invention.
- FIG. 8 is a vertical sectional view showing a second example of a detection unit used for detecting a reaction product in the reaction container kit of the present invention.
- Fig. 9 is a vertical sectional view showing a third example of a detection unit used for detecting a reaction product in the reaction container kit of the present invention.
- FIGS. 10 (A) to 10 (B) are diagrams showing other embodiments of the reaction container kit, FIG. 10 (A) is a vertical sectional view, and FIG. It is a top view which shows an injection tip.
- FIG. 11 is a vertical sectional view showing an example of a detection unit used for detecting a reaction product in the reaction container kit of the same example together with the reaction container.
- FIGS. 12 (A) to 12 (B) are views showing still another embodiment of the reaction vessel kit, FIG. 12 (A) is a vertical sectional view, and FIG. 12 (B) is a reaction plate. It is a top view which shows a dispensing tip.
- FIG. 13 is a vertical sectional view showing an example of a detection unit used for detecting a reaction product in the reaction container kit of the same example together with the reaction container.
- FIG. 14 is a vertical sectional view showing still another embodiment of the reaction container kit together with an example of a detection unit used for detection of reaction products.
- FIG. 15 is a vertical sectional view showing another embodiment of a reaction container kit.
- FIGS. 16 (A) to 16 (C) are views showing still another embodiment of the reaction container kit, FIG. 16 (A) is a vertical sectional view, and FIG. 16 (B) is a reaction plate.
- FIG. 16C is a plan view showing a dispensing tip, and FIG. 16C is an external perspective view.
- FIG. 17 (A) to FIG. 17 (C) are views showing still another embodiment of the reaction vessel kit, FIG. 17 (A) is a vertical sectional view, and FIG. 17 (B) is a reaction plate.
- FIG. 17C is a plan view showing a dispensing tip, and FIG. 17C is an external perspective view.
- FIGS. 18 (A) to 18 (C) are views showing still another embodiment of the reaction vessel kit, FIG. 18 (A) is a vertical sectional view, and FIG. 18 (B) is a reaction plate.
- FIG. 18C is a plan view showing a dispensing tip, and FIG. 18C is an external perspective view.
- FIGS. 19 (A) to 19 (C) are views showing still another embodiment of the reaction vessel kit, FIG. 19 (A) is a vertical sectional view, and FIG. 19 (B) is a reaction plate.
- FIG. 19C is a plan view showing a dispensing tip, and FIG. 19C is an external perspective view.
- FIG. 20 is an internal schematic perspective view showing an example of a reaction vessel treatment apparatus.
- FIG. 21 is a block diagram showing a control system in the reaction vessel processing apparatus.
- Fig. 1 (A) to Fig. 1 (C) are perspective views showing a reaction container kit of one embodiment
- Fig. 1 (A) is a state before a sample is injected
- Fig. 1 (B) is a diagram
- Fig. 1 (C) shows the state where the first bar code label is peeled off to inject the sample
- Fig. 1 (C) shows the state where the second bar code label is applied after the sample is injected.
- Fig. 2 (A) to Fig. 2 (C) specifically show the internal structure of this example.
- Fig. 2 (A) is a vertical sectional view and Fig. 2 (B) is a reaction plate and a separation plate.
- FIG. 2C is a schematic cross-sectional view showing another example of the dispensing tip.
- the reaction plate 2 contains a reaction part 4 for causing the sample to react on the surface side of the substrate 3 and a reagent used for the sample reaction.
- a reagent container 12 sealed with 4 is provided.
- the reaction part 4 is provided as a recess on the surface of the substrate 3. If the temperature of the reaction part 4 is controlled from the outside during the reaction, it is preferable that the thickness of the reaction part 4 in that part is thin in order to improve the thermal conductivity.
- the reagent container 12 is composed of a plurality of recesses formed in the substrate 3, and the necessary reagents are accommodated in these recesses and covered with a film 14 that can be penetrated by a dispensing tip 20 described later.
- the film 14 is, for example, an aluminum foil, a laminated film of a resin film such as aluminum and PET (polyethylene terephthalate) film, and is attached by fusion or adhesion so that it does not easily peel off.
- a mixing portion for mixing the sample and the reagent as needed is also provided with a recess.
- Such a mixing portion that can be formed by the process can be covered with the film 14 in an empty state.
- the reaction unit 4 itself can be used as a detection unit by irradiating the reaction unit 4 with external force.
- the detection unit can be provided independently of the reaction unit 4.
- the reaction solution after the reaction between the sample and the reagent is dispensed by the dispensing tip 20, and each reagent for detecting the state after the reaction is previously detected.
- Such a detection part may also have its surface covered with a film that can be penetrated by the dispensing tip 20. Similar to film 14, such a film can also be made of aluminum foil, a laminated film of aluminum and a resin film such as PET film, etc., and fused and adhered by adhesion so that it does not easily peel off. be able to.
- the material of the substrate 3 including the reaction section 4 is not particularly limited, but it is preferable that there is a material that can be obtained at low cost because the reaction vessel can be used.
- a resin material such as polypropylene and polycarbonate is preferable.
- a light-transmitting resin is used to enable optical detection from the bottom side. Preferably it is formed.
- the substrate 3 when performing fluorescence detection, is made of a material such as a low autofluorescence material (low emission of fluorescence from itself !, a property) and a light transmissive resin such as polycarbonate. Preferably it is.
- the thickness of the substrate 2 is 0.3 to 4 mm, preferably 1 to 2 mm. From the viewpoint of low autofluorescence for fluorescence detection, the thickness of the substrate 3 is preferably thin.
- a dispensing tip 20 is disposed on the upper surface side of the reaction plate 2.
- the dispensing chip 20 further dispenses the reaction solution after the reaction to the detection unit.
- the dispensing tip 20 includes a syringe 22, and the external force of the cover 24 performs a dispensing operation by driving the syringe 22.
- the dispensing tip 20 may be provided with an internal internal filter 23 instead of the syringe 22.
- the filter absorbs foreign matter entering from the outside and covers it. It is more effective in preventing foreign matter from entering the space covered with 24 and preventing reactants and reaction products from being released to the outside from the space covered with cover 24. .
- the cover 24 is provided so as to cover the upper space on the surface side of the reaction plate 2.
- the cover 24 is composed of a cover body 26 that covers the periphery and a bellows film 28 that covers the upper part, and blocks the space on the surface side of the reaction plate 2 from external force.
- the cover body 26 is assembled integrally with the reaction plate 2 through a force with the lower end portion fixed to the reaction plate 2 or a seal material, and maintains the shape of the cover 24 with rigidity.
- the bellows film 28 also has a flexible diaphragm and a flexible film force.
- the tip 20 of the dispensing tip 20 is inside the space covered with the cover 24, and the base end is outside the space covered with the cover 24. Hold it so you can move!
- the material of the cover 24 is not particularly limited as long as it can cover the upper space on the surface side of the reaction plate 2 in an airtight manner, but the reaction vessel is disposable. Therefore, it is preferable that there is a material that can be obtained at a low cost.
- the cover body 26 is preferably made of a resin such as polypropylene or polycarbonate
- the bellows film 28 is preferably made of nylon (registered trademark), polyvinyl chloride, vinyl rubber, silicone rubber or other rubber materials. .
- a part of the cover body 26 or the substrate 3 is provided with a holding member 30 for holding the dispensing tips 20 before and after use. It is removed from the upper part of the reaction plate 2 so that it can move freely on the upper surface.
- an opening 31 force is provided in a part of the cover body 26, and a sample container 32 is attached to the opening 31 so as to be openable and closable.
- the reaction container is a first barcode that covers the sample container 32 outside the cover body 26 as shown in FIG. 1 (A).
- Label 1 30 is pre-applied.
- the first bar code label 130 is for reading before dispensing the sample into the reaction vessel, and it shows data specific to the reaction vessel and that no sample has been injected into the reaction vessel. Is indicated by barcode 132. Is recorded.
- the barcode 132 of the first barcode label is read by the barcode reader, and the reaction container is a reaction container for the test item requested for the sample to be injected. Automatically determine whether or not the reaction vessel is still a sample that has been injected.
- the opening 31 cannot be opened unless the first barcode label 130 is peeled off.
- the reaction vessel is further provided with a second barcode label 134 for reading after dispensing of the sample.
- a part of the second barcode label 134 is attached to the reaction container so that it can be attached to the reaction container, and the adhesive surface is covered with a release paper. By removing the release paper, the bar code label 134 can be attached to the reaction container, the sample container 32 can be covered, and the opening 31 can be sealed.
- the bar code label 134 data indicating that a sample has already been injected into the reaction container is recorded by the bar code 136 (see FIG. 1 (C)).
- the back surface of the bar code labels 130, 134 (the surface on which the bar code is printed is the front surface.
- a specific example of the bar code labels 130 and 134 is one in which an adhesive is applied to a base material.
- the substrate polyethylene film, polypropylene film, polystyrene film, synthetic paper, polyimide film, variable information film and the like can be used.
- the adhesive applied to the substrate PVA emulsion, SBR emulsion, acrylic emulsion, synthetic rubber emulsion, pressure sensitive adhesive, heat sensitive adhesive, and the like can be used. Since the barcode label 130 is peeled off when the sample is injected, it is preferable that the adhesive applied to the substrate is an adhesive that can be easily peeled off.
- the sample container 32 is formed with a recess opened upward for injecting the sample.
- the plate 34 holding the sample container 32 closes the opening 31.
- the release paper on the adhesive surface of the bar code label 134 is peeled off, and the bar code label 134 is attached to the cover body 26 so as to cover the plate 34 with the bar code label 134. This will cause the bar code label 134 As a result, the opening 31 is sealed.
- This reaction vessel is disposable, and after the analysis of one sample, the entire reaction vessel is discarded with the reaction plate 2 covered with the cover 24.
- the reaction vessel before use is supplied in the state shown in Fig. 1 (A). Read the barcode 132 of the first barcode label with a barcode reader before sample injection, and whether the reaction container is the reaction container for the test item requested for the sample to be injected. Is automatically determined. When the reaction container is correct, the sample container 32 appears as shown in FIG. 1 (B) when the first bar code label 130 is removed. Pull out the sample container 32, inject the sample into it, and return the sample container 32 to the reaction container again.
- the release paper of the second barcode label 134 is peeled off, and the second barcode label 134 is affixed on the sample container 32.
- the opening 31 is sealed by the second bar code label 134, and the sample force is shut off from the outside while being introduced into the space covered by the cover 24 of the reaction vessel.
- the barcode 136 Since data indicating that the sample has already been injected into the reaction vessel is recorded on the second barcode label 134 by the barcode 136, the barcode 136 is recorded with the barcode reader. By reading, it can be automatically determined that a sample has already been injected into the reaction vessel!
- a bar code label 138 indicated by a chain line in Fig. 1 (A) is a part of the first bar code label in another embodiment.
- the first barcode label is peeled off! /, Part 130 and part 138 that is not peeled off during sample dispensing, and is affixed to the reaction container without being peeled off.
- data indicating information specific to the reaction container such as items to be inspected in the reaction container is recorded by the barcode 140, and in the part 130 to be removed, the reaction container is recorded.
- the bar code 132 records data indicating that the sample has not been injected yet.
- the sample injection method is the same as in the case of a reaction vessel without this part 138. Portion 138 remains attached to the reaction vessel without being peeled after sample injection.
- the bar code label is not shown. However, as shown in the embodiment of FIG.
- the first barcode label 130 covering the sample container is attached in advance, and a part of the first barcode label 134 is attached to the reaction container so that the second barcode label 134 can be attached to the reaction container. Further, a bar code label portion 138 that remains attached to the reaction container may be provided.
- FIG. 3 shows a state in which the drive unit 36 starts engaging the dispensing tip 20 and the syringe 22 with the sample introduced.
- the plunger holder 36 b that is a syringe drive unit is lowered and engaged with the plunger of the syringe 22.
- the tip holder 36 a is also lowered and press-fitted into the dispensing tip 20 to hold the dispensing tip 20.
- the dispensing tip 20 is removed from the holding unit 30.
- the dispensing tip 20 can move freely while being blocked from the outside by the bellows film 28.
- the dispensing tip 20 is moved to the sample in the sample container 32, and the sample is injected and dispensed to the reaction unit 4.
- the dispensing tip 20 is moved to the reagent container 12, penetrates the film 14, dispenses the reagent from the reagent container 12 to the reaction unit 4, and is used for the reaction.
- the reaction unit 4 is brought into contact with an external heat source as necessary, and is controlled to a predetermined temperature.
- reaction product is detected.
- the external force of the reaction plate 2 is optically detected while the reaction product is in the reaction section 4.
- a detection unit is disposed below the reaction unit 4 and detection is performed by optical or other means.
- the reaction plate 2 includes the reagent container 12, but the reaction plate 2 may not include the reagent container 12. In that case, the reagent is supported with the sample. It can be used by being injected into the sample container 32 and introduced into the reaction container, or introduced into another reaction container (not shown) and introduced into the reaction container.
- FIG. 7 to FIG. 9 show examples of detection units used for detecting reaction products in the reaction container in the reaction container kit of the present invention.
- Figure 7 shows an example of a detection unit that also has absorbance detector power.
- the reaction section 4 includes a pair of planes parallel to each other as an entrance surface and an exit surface of the measurement light.
- the detection unit 38a includes a light source 40a as an irradiation optical system, a pair of lenses 42a for condensing the light from the light source 40a, converging the light once into parallel light, and then condensing and irradiating the reaction unit 4;
- the light force from the light source 40a which is arranged in the part of the parallel light between the pair of lenses 42a, selects the light with a predetermined wavelength to be the measurement light 44a, and guides the measurement light to the incident surface of the reaction unit 4.
- Mira 1 46 is located on the optical path.
- the light source 40a in addition to a lamp light source such as a tungsten lamp that generates light having a wavelength in the ultraviolet region to the visible region, a light emitting diode (LED) or a laser diode (LD) is used.
- a lamp light source such as a tungsten lamp that generates light having a wavelength in the ultraviolet region to the visible region
- a light emitting diode (LED) or a laser diode (LD) is used as a light-receiving optical system.
- the photodetector 48a, the mirror 50 that guides the light exiting the exit surface of the reaction unit 4 to the photodetector 48a, and the light is collimated and then collected and detected.
- a pair of lenses 52 to be incident on the detector 48a and a filter 54a for selecting a predetermined wavelength suitable for measurement by being arranged in a portion where the light is collimated between the pair of lenses 52 are arranged on the optical path! RU
- the reason why the respective lights are made parallel light by the lenses 42a and 52a is to improve the accuracy of wavelength selection in the filters 44a and 54a.
- the light power from the light source 40a is also selected by a filter 44a, 54a for a wavelength suitable for detecting the reaction product, and the absorbance at that wavelength is measured to detect the reaction product.
- FIG. 8 shows an example of a detection unit including a fluorescence detector.
- This detection unit 38b is a light source 40b as an excitation optical system, a pair of lenses 42b for collecting and irradiating the light from the light source 40b, collecting the light from the light source 40b, and then collimating and irradiating the reaction unit 4 and the lens 42b.
- a filter 44b that is arranged in the optical path of the light beam and has a light source power that selects a predetermined excitation light wavelength is provided.
- the photo detector 48b as a light receiving optical system and the fluorescence generated from the reaction section 4 are received and once converted into parallel light, then condensed and detected.
- the 48b is provided with a pair of lenses 52b for incident light, and a filter 54b for selecting a predetermined fluorescence wavelength, which is disposed in the optical path of fluorescence converted into parallel light by the lens 52b.
- the reason why the lenses 42b and 52b make the respective lights collimated is to improve the accuracy of wavelength selection in the filters 44b and 54b.
- the wavelength of the excitation light for exciting the reaction product by the filter 44b is selected from the light from the light source 40b, and the reaction product in the reaction section 4 is irradiated to the reaction product. Fluorescence generated from the light is received by the light receiving optical system, a predetermined fluorescence wavelength is selected by the filter 54b, and the fluorescence is detected by the photodetector 48b.
- FIG. 9 is an example of a detection unit for detecting chemiluminescence or bioluminescence from a reaction product.
- This detection unit 38c includes a light detector 48c for detecting light emitted from the reaction unit 4, a lens 52c for receiving light emitted from the reaction unit 4 and guiding it to the light detector 48c, and the collected light.
- a filter 54c for selecting a predetermined emission wavelength is also provided.
- chemiluminescence or bioluminescence light from the reaction product in the reaction section 4 is collected by the lens 52c, the wavelength is selected by the filter 54c, and detected by the photodetector 48c.
- FIGS. 10 to 14 show other examples in which the structure of the reaction plate is different.
- the reaction product is detected by the reaction unit 4.
- the reaction plate further includes an analysis unit for analyzing the reaction product. I have.
- the reaction plate 2a in the embodiment of Fig. 10 includes an electrophoresis section as an analysis section.
- An example of the electrophoresis part is an electrophoresis chip 100, which includes a reaction product injection part 103, an electrophoresis separation channel 102, and electrophoresis voltage application electrodes 106a to 106d.
- the sample separation channel 104 intersects with the electrophoresis separation channel 102 and introduces the sample into the electrophoresis separation channel 102.
- the electrophoresis chip 100 has a low autofluorescence and light-transmitting resin, for example, glass such as polycarbonate. Or it is made of a material such as quartz.
- the reaction plate 2a also includes a separation buffer solution container 15 that contains a separation buffer solution injected into the flow paths 102 and 104 and is sealed with a film that can be inserted at the tip of the dispensing tip 20 on the surface side thereof. I have.
- Electrophoresis voltage application electrodes 106a to 106d are connected to the ends of the flow paths 102 and 104, respectively, and are guided to the outside of the cover 24 so that they can be connected to a power supply device provided outside the reaction vessel. Yes.
- Reservoirs are provided at the ends of the flow paths 102 and 104, and the separation buffer solution stored in the separation buffer solution container 15 is placed in these reservoirs.
- the reagent container 12 contains a PCR reaction reagent.
- Reaction part 4 becomes the PCR reaction part.
- the sample is introduced from the sample container 32, and the reaction container is attached to the processing apparatus.
- the dispensing tip 20 dispenses from the sample container 32 to the reaction section 4, and the dispensing tip 20 dispenses the PCR reaction reagent from the reagent container 12 to the reaction section 4 and further onto it.
- the reaction solution in the reaction section 4 is controlled to a predetermined temperature cycle to cause a PCR reaction.
- the separation buffer liquid is supplied from the separation buffer liquid container 15 to the flow paths 102 and 104 via the reservoir of the electrophoresis chip 100 by the dispensing chip 20.
- the reaction solution after completion of the PCR reaction is injected as a sample from the reaction section 4 into the injection section 103 of the electrophoresis chip 100 in the separation buffer liquid supply section by the dispensing chip 20. Thereafter, a voltage is applied to the channels 102 and 104 by the electrodes 106a to 106d from the power supply device 101 (see FIG. 11) provided in the processing apparatus, and the sample is introduced into the electrophoresis separation channel 102, and thereafter Electrophoretic separation flow path 102 is migrated and separated.
- the processing apparatus is provided with a detection unit 38d.
- a PCR reaction unit may be provided separately from the force reaction unit 4 using the reaction unit 4 as a PCR reaction unit.
- the detection unit 38d is shown in FIG.
- the detection unit 38d includes an excitation optical system and a fluorescence receiving optical system, and performs fluorescence detection of a sample component passing through a predetermined position of the electrophoresis separation channel 102. Since the detection unit 38d detects the fluorescence of the sample component passing through the fixed position, the detection unit 38d does not need to be moved.
- the excitation optical system includes a light source 40c, a lens 42c that collects light from the light source 40c to make parallel light, and is arranged in the optical path of the light beam that has been made parallel light by the lens 42c. And a filter 44c for selecting the excitation light wavelength.
- a dichroic mirror 53 and an objective lens 55 are provided.
- the dichroic mirror 53 is configured to reflect light having an excitation light wavelength used in this embodiment and transmit light having a fluorescence wavelength.
- the fluorescence receiving optical system is arranged at a position for receiving the fluorescence that has been made parallel light by the objective lens 55 and transmitted through the dichroic mirror 53, and the fluorescence power transmitted through the dichroic mirror 53 has a predetermined fluorescence wavelength.
- a filter 54c to be selected and a lens 52c that collects the fluorescence selected by the filter 54c and enters the detector 48c are provided. Again, the reason why the lenses 42 C 55 make the respective lights collimated is to improve the accuracy of wavelength selection in the filters 44c and 54c.
- the light force from the light source 40c also selects the wavelength of the excitation light for exciting the reaction product by the filter 44c, and passes through a predetermined position in the electrophoresis separation channel 102.
- the product is irradiated, the fluorescence generated from the reaction product is received by the light receiving optical system, a predetermined fluorescence wavelength is selected by the filter 54c, and the fluorescence is detected by the photodetector 48c.
- the reaction plate 2b in the example of FIG. 12 includes a DNA chip 110 as an analysis unit.
- a probe that reacts with a gene when the reaction product contains a gene is fixed to the DNA chip 110.
- the DNA chip 110 is formed of a low autofluorescence and light-transmitting resin such as polycarbonate or glass in order to detect fluorescence on the back side.
- reaction plate 2a was bonded to the probe on the DNA chip 110 on the surface side thereof.
- a cleaning liquid container 17 is also provided, which stores a cleaning liquid for separating and removing the reaction product that has not been bound from the reaction product and is sealed with a film that can be inserted at the tip of the dispensing tip 20.
- the reagent container 12 contains a PCR reaction reagent.
- Reaction part 4 becomes the PCR reaction part.
- the sample is introduced from the sample container 32, and the reaction container is attached to the processing apparatus.
- the dispensing tip 20 dispenses from the sample container 32 to the reaction section 4, and the dispensing tip 20 dispenses the PCR reaction reagent from the reagent container 12 to the reaction section 4 and further onto it.
- the reaction solution in the reaction section 4 is controlled to a predetermined temperature cycle to cause a PCR reaction.
- reaction solution after completion of the PCR reaction is injected as a sample from the reaction section 4 into the DNA chip 110 by the dispensing chip 20.
- washing solution is injected from the washing solution container 17 into the DNA chip 110 by the dispensing tip 20, and the reaction product not bound to the probe is sucked together with the washing solution by the dispensing tip 20 and removed.
- the reaction product bound to the probe can be detected by fluorescence. Thereby, it is detected that the gene corresponding to the probe at the position where the fluorescence was detected was included in the sample.
- the processing apparatus is provided with a detection unit 38e!
- the detection unit 38e is shown in FIG.
- the configuration of the optical system of the detection unit 38e is the same as that of the detection unit 38d shown in FIG.
- This detection unit 38e must move over the position of the probe arranged on the DNA chip 110! Therefore, the detection unit 38e is supported so as to be movable, and is different from the detection unit 38d shown in FIG.
- the movement can be realized by the movement of the table 82 in the X direction and the movement of the detection unit 38e in the Y direction as shown in FIG.
- the reaction plate 2c in the example of FIG. 14 includes a DNA chip 120 as an analysis unit.
- the DNA chip 120 is implemented as shown in FIG. Different from the DNA chip 110 in the example. A phenomenon is used in which the current value of the probe changes depending on whether or not the sample gene is bound to the probe. Since the DNA chip 120 does not perform optical detection, it does not have to be made of a light-transmitting material.
- a probe that reacts with the gene is immobilized on the DNA chip 120. From each of these probes, an electrode is taken out on the back side, and the current value of each flow is measured. In this example, it is not necessary to label the sample with a fluorescent substance.
- the electrodes from which the probe forces are also extracted on the back side are connected to a detector 122 provided in the processing apparatus, and the current values of the probes are measured.
- the reaction plate 2c also contains a cleaning solution on its surface side for separating and removing the reaction product that has not been bound from the reaction product bound to the probe in the DNA chip 120 at the tip of the dispensing tip 20.
- a cleaning liquid container 17 sealed with an insertable film is provided.
- Reagent container 12 contains a PCR reaction reagent. Reaction part 4 becomes the PCR reaction part.
- the sample is introduced from the sample container 32, and the reaction container is attached to the processing apparatus.
- the dispensing tip 20 dispenses from the sample container 32 to the reaction section 4, and the dispensing tip 20 dispenses the PCR reaction reagent from the reagent container 12 to the reaction section 4 and further onto it.
- the reaction solution in the reaction section 4 is controlled to a predetermined temperature cycle to cause a PCR reaction.
- reaction solution after completion of the PCR reaction is injected as a sample from the reaction section 4 into the DNA chip 120 by the dispensing chip 20. Thereafter, the washing solution is injected from the washing solution container 17 into the DNA chip 120 by the dispensing tip 20, and the reaction product not bound to the probe is sucked and removed together with the washing solution by the dispensing tip 20.
- the processing apparatus is provided with a detector 122, which removes the reaction product that does not bind to the probe, and detects the detector. The current value of each probe is measured by 122.
- the DNA chips 110 and 120 are placed in the area for noise visualization.
- the gene can be measured in the same manner even if the area is changed.
- FIG. 15 shows another embodiment having a different cover structure.
- the partial force of the cover to support the dispensing tip 20 movably and cover the top of the reaction plate 2 was the bellows film 28 in the example of Fig. 1, whereas it was flexible in the example of Fig. 15. It differs in that it is a deformed film material 28a.
- As the film-like material 28a nylon (registered trademark), polyvinyl chloride vinyl, silicone rubber, and other rubber materials are preferred, as with the bellows film 28.
- one side of the sample container is rotatably supported by the cover body 26, whereas the sample container 32a in the embodiment of FIG. It differs in that it is slidably mounted. Even in such a sample container 32a, the sample container 32a can be dispensed to the sample container 32a by being pulled out from the cover body 26 to the outside.
- a bar code label 134 (see FIG. 1) is provided that is attached to the cover so as to seal the opening 31 in a state where the sample is injected into the space where the sample container 32a is covered with the cover. The method for sealing the opening 31 by the bar code label 1 34 is the same as that in the embodiment of FIG.
- These detection units 38a, 38b, and 38c are arranged so as to be below the reaction plate 2 in the processing apparatus for processing the reaction container, with the reaction container mounted on the processing apparatus. ing.
- FIG. 16 shows still another embodiment of the reaction container kit.
- (A) is a vertical sectional view
- (B) is a horizontal sectional view
- (C) is an external perspective view.
- the cover that movably supports the dispensing tip 20 is made of a rigid material.
- the cover body 60 of the cover 24a has an opening 62 above the reaction plate 2, and the opening 62 is provided with a cover plate 64 for movably supporting the dispensing tip 20 within the range of the opening 62.
- the cover body 60 has a double structure with a gap around the opening 62, and the cover plate 64 has a sealing material 66 around the opening 62, and the sealing material 66 has two parts around the opening 62 in the cover body 60.
- the cover plate 64 can move in the X direction in the horizontal plane by moving in the X direction between the heavy structure gaps. Dispensing tips 20 are placed on the cover plate 64 in the vertical direction through other sealing materials 68 ( It is slidably supported in the z direction).
- the cover plate 64 moves in a horizontal plane while being kept airtight by the sealing structure between the sealing material 66 and the double structure gap above the cover body 60, and the dispensing chip 20 is sealed.
- the dispensing chip 20 can freely move in the upper space of the reaction plate 2 in both the vertical and horizontal directions.
- FIG. 17 shows still another embodiment.
- the cover plate 64 can move in both X and Y directions, and the number of reagent containers 12 in the reaction plate 2 is increased. Are the same.
- FIG. 18 shows still another embodiment.
- the cover plate 64a constituting the upper member of the cover in order to move the dispensing tip 20 in the in-plane direction, the cover plate 64a constituting the upper member of the cover is supported so as to be rotatable in the in-plane direction.
- the cover plate 64a has a disc shape, and a sealing material 66 is attached around the cover plate 64a.
- the sealing material 66 is supported by a double-structured gap provided in the upper part of the cover body 60, and supports the cover plate 64a so as to be rotatable while maintaining airtightness.
- the dispensing tip 20 is supported on the cover plate 64a by a sealant 68 so as to be movable in the vertical direction, and the position where the dispensing tip 20 is supported is a position deviated from the rotational center force of the cover plate 64a.
- the cover plate 64a rotates, the position of the dispensing tip 20 moves on the circumference around the rotation center of the cover plate 64a.
- the arrangement is determined so that the reaction unit 4, the reagent container 12, and the sample container 32 are positioned on the movement trajectory of the dispensing tip 20.
- FIG. 19 shows still another embodiment.
- the cover plate 64a also has an opening 70, and the periphery of the opening 70 has a double structure, and the other cover plate 71 is interposed in the gap of the double structure through a sealant 72. Is supported movably.
- the dispensing tip 20 is supported on the cover plate 71 by another sealing material 68 so as to be movable in the vertical direction.
- the dispensing tip 20 can be moved in the in-plane direction by the sealing material 72. Therefore, the moving range of the dispensing tip 20 depends on the rotation of the cover plate 64a.
- the donut-shaped range around the rotation center of the cover plate 64 a can be moved by both the circumference and the range of movement in the horizontal plane in which the small cover plate 71 can be moved by the sealant 72. In this way, the movement range of the dispensing tip 20 is widened, so that the number of reaction units 4 and reagent containers 12 arranged in the movement range can be increased, and the arrangement of these containers including the sample container 32 can be increased.
- the degree of freedom for is increased.
- FIG. 20 is a perspective view schematically showing the inside of an example of a processing apparatus for processing the reaction container kit according to the present invention.
- the reaction container 80 represents the reaction vessel kit shown in the above-mentioned Examples.
- the reaction container 80 is mounted on a table 82 which is a reaction container mounting portion.
- the table 82 has an opening on the lower surface side of the reaction vessel 80, and a detection unit 38 for optically detecting the reaction product in the reaction section 4 of the reaction vessel 82 is disposed below the table 82.
- a temperature control unit 83 for controlling the temperature of the reaction vessel 82 is also arranged on the table 82.
- the temperature control unit 83 controls the temperature for the gene amplification reaction.
- the temperature control unit 83 performs temperature control of the analysis unit.
- the temperature control unit 83 includes those having both functions.
- the detection unit 38 is the one shown in FIGS.
- the table 82 moves in the front-rear direction (X direction), while the detection unit 38 is supported so as to move in the lateral direction (Y direction) orthogonal thereto.
- a drive unit 36 for driving the dispensing tip 20 is mounted so as to be movable in the Y direction and the Z direction. As shown in FIG. 3, the drive unit 36 is engaged with the base end of the dispensing tip 20 to hold the dispensing tip 20, and a syringe provided on the dispensing tip 20.
- a syringe drive unit 36b that engages with the plunger 22 and drives the syringe is provided on the same axis so that both the movement of the dispensing tip 20 and the drive of the syringe 22 can be performed.
- FIG. 21 is a block diagram showing a control system in an example of the reaction vessel processing apparatus.
- a control unit 84 composed of a dedicated computer (CPU) or a general-purpose personal computer is provided. Yes.
- the control unit 84 moves and dispenses the dispensing tip 20 by the drive unit 36 engaged with the proximal end of the dispensing tip 20, controls the temperature by the temperature control unit 83, and measures the reaction unit 4 of the reaction vessel 80.
- the detection operation by the detection unit 38 that optically detects the reaction product by irradiating light or excitation light is controlled.
- the bar code label 134 is not shown.
- the sample was injected into the space where the sample container was covered with the cover outside the cover body.
- a seal member that covers the outside of the sample container and is attached to the cover body is provided in common.
- control unit 84 In order to use the control unit 84 as an input unit for operating an external force or as a monitor for displaying an inspection result, the control unit 84 is provided with an external computer such as a personal computer (PC) 86. You may connect.
- PC personal computer
- the present invention can be used for measurement of various chemical reactions and biochemical reactions.
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- Life Sciences & Earth Sciences (AREA)
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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JP2008515517A JP4985646B2 (ja) | 2006-05-11 | 2007-05-10 | 反応容器キット |
US12/298,029 US20090098025A1 (en) | 2006-05-11 | 2007-05-10 | Reaction container kit |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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JP2006-132055 | 2006-05-11 | ||
JP2006132055 | 2006-05-11 |
Publications (1)
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WO2007132740A1 true WO2007132740A1 (ja) | 2007-11-22 |
Family
ID=38693834
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PCT/JP2007/059687 WO2007132740A1 (ja) | 2006-05-11 | 2007-05-10 | 反応容器キット |
Country Status (4)
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US (1) | US20090098025A1 (ja) |
JP (1) | JP4985646B2 (ja) |
CN (1) | CN101443441A (ja) |
WO (1) | WO2007132740A1 (ja) |
Cited By (6)
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JP2011209124A (ja) * | 2010-03-30 | 2011-10-20 | Toppan Printing Co Ltd | 前処理器具 |
JP2014103866A (ja) * | 2012-11-26 | 2014-06-09 | Dainippon Printing Co Ltd | 微生物培養具、微生物培養具に検体情報を印刷する印刷システムおよび印刷方法、コンピュータを印刷システムの制御装置として機能させるためのプログラム、および、プログラムを格納した記録媒体 |
JP2014103865A (ja) * | 2012-11-26 | 2014-06-09 | Dainippon Printing Co Ltd | 微生物培養具 |
JP2015010836A (ja) * | 2013-06-26 | 2015-01-19 | 栗田工業株式会社 | 溶存成分の濃度測定装置 |
JP2017074076A (ja) * | 2017-02-03 | 2017-04-20 | 大日本印刷株式会社 | 微生物培養具 |
JP2017079793A (ja) * | 2017-02-03 | 2017-05-18 | 大日本印刷株式会社 | 微生物培養具、微生物培養具に検体情報を印刷する印刷システムおよび印刷方法、コンピュータを印刷システムの制御装置として機能させるためのプログラム、および、プログラムを格納した記録媒体 |
Families Citing this family (1)
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US10885412B2 (en) * | 2018-08-28 | 2021-01-05 | Trimble Inc. | Systems and methods for tracking produce |
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JP2011209124A (ja) * | 2010-03-30 | 2011-10-20 | Toppan Printing Co Ltd | 前処理器具 |
JP2014103866A (ja) * | 2012-11-26 | 2014-06-09 | Dainippon Printing Co Ltd | 微生物培養具、微生物培養具に検体情報を印刷する印刷システムおよび印刷方法、コンピュータを印刷システムの制御装置として機能させるためのプログラム、および、プログラムを格納した記録媒体 |
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JPWO2007132740A1 (ja) | 2009-09-24 |
JP4985646B2 (ja) | 2012-07-25 |
CN101443441A (zh) | 2009-05-27 |
US20090098025A1 (en) | 2009-04-16 |
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